Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	989,945	A→G	19.3%	S138P (TCA→CCA)	pncB ←	nicotinate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	989,945	0	A	G	19.3%	144.8 / 7.5	85	S138P (TCA→CCA)	pncB	nicotinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (33/32);  new base G (8/10);  total (41/44)
Fisher's exact test for biased strand distribution p-value = 7.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACC‑AGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGAC‑TAATTTGCTTTC‑CAGCGTGTC‑GAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTGA  >  NC_000913/989709‑990183                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 tCCCATCGGCGTGAGGGAAAGCCGACGCGACAGATCGTAGTTGCTGGTGCCCACAAACC‑AGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGAC‑TAATTTGCTTTC‑CAGCGTGTC‑GAGGGCTTGCGCAACGTCGGCCTGCGGTGag                                                                                                                                                                                                                                                 <  2:1413102/238‑1 (MQ=255)   ccATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACC‑AGGATTCCTGTTGCAGACGCGTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGAC‑TAATTTGCTTTC‑CAGCGTGTC‑GAGGGCTTGCGCAACGTCGGCCTGCGGTGagcga                                                                                                                                                                                                                                              <  1:255405/239‑1 (MQ=255)    cATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACC‑AGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACGCCGAGGAGTCGAC‑TAATTTGCTTTC‑CAGCGTGTC‑GAGGGCTTGCGCAACGTCGGCCTGCGGTGagcgat                                                                                                                                                                                                                                             <  2:505927/239‑1 (MQ=255)      tCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACC‑AGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGAC‑TAATTTGCTTTC‑CAGCGTGTC‑GAGGGCTTGCGCAACGTCGGCCTGCGGTGagcgatag                                                                                                                                                                                                                                           <  2:1553078/239‑1 (MQ=255)        ggCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACC‑AGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACTCCGCGAAGACGGA‑CAATTTTCTTTC‑CCGCGCGTG‑CGGGGCGTTCGCCACGCCGGCACGCGGGGggtgatcgcg                                                                                                                                                                                                                                         >  1:1350585/1‑239 (MQ=255)        ggCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACC‑AGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGAC‑TAATTTGCTTTC‑CAGCGTGTC‑GAGGGCTTGCGCAACGTCGGCCTGCGGTGagcgatagcg                                                                                                                                                                                                                                         <  1:662487/239‑1 (MQ=255)        ggCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACC‑AGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGGTAACACCCAGAAGGCGAA‑TAATTTGCTTTT‑CAGCGTGTCAGGGGGTTTCGGCACCGGCGGCC‑GCGGGGagcggtagcc                                                                                                                                                                                                                                         >  1:635591/1‑238 (MQ=255)        ggCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACC‑AGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAAGCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGAC‑TAATTTGCTTTC‑CAGCGTGTC‑GAGGGCTTGCGCAACGTCGGCCTGCGGTGagcgatagcg                                                                                                                                                                                                                                         <  1:1712364/239‑1 (MQ=255)          cGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACC‑AGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGAC‑TAATTTGCTTTC‑CAGCGTGTC‑GAGGGCTTGCGCAACGTCGGCCTGCGGTGagcgatagcga                                                                                                                                                                                                                                        <  1:626044/238‑1 (MQ=255)          cGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACC‑AGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGGTAAAGCCCAGAAGTCGGC‑TAATTTTCGTTC‑CAGCGCGTC‑GCGGGCTTGCGCCACGTCGGGCTGCGGGGgtcgatagcgat                                                                                                                                                                                                                                       >  1:618772/1‑239 (MQ=255)           gTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACC‑AGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGAC‑TAATTTGCTTTC‑CAGCGTGTC‑GAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATg                                                                                                                                                                                                                                      <  1:540331/239‑1 (MQ=255)           gTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACC‑AGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGAC‑TAATTTGCTTTC‑CAGCGTGTC‑GAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATg                                                                                                                                                                                                                                      <  2:609224/239‑1 (MQ=255)              aGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACC‑AGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGAC‑TAATTTGCTTTC‑CAGCGTGTC‑GAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTa                                                                                                                                                                                                                                    <  2:618772/238‑1 (MQ=255)                         aCGCGCCAGATCGTAGTTGCTGGTGCCCACAAACC‑AGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACGCCCAGAAGTCGAC‑TAATTTTCTTTT‑CAGCGTGTC‑GAGGGCTTGCGCAACGACGGCCCTCGGGGAGCGATAGCGCTGTACCCTTTCACTGa                                                                                                                                                                                                                        >  2:1071719/1‑239 (MQ=255)                                       ggtggtgggTGCCTACAAACA‑GTGATTCCTGTTGCCGTCGCTACGCGATGTTTTCTTTTCCTACGCAAGAAAAAG‑GGCGACGGGTGACAAAACCCATCAGATGGAATCTCT‑ACATAGCAAGCCCGGCGGTTACCGCCGAGAAGTCGAC‑TAATTTGCTTTC‑CGGCGTGTC‑GGGGGCTTGCGCAACGTCGGCCTGCGGTGAGTTTTAGCGATGTACCATTCCTCTGATAACCGCCAGCaa                                                                                                                                                                                                           <  1:332295/231‑1 (MQ=255)                                         ttGCTGGTGCCCACAAACC‑AGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAAAGCCGAGAAAACGAC‑CAATTTGCTTTT‑CAGCGGGTC‑GAGGGCTTGCGCAACGTCGGGCTGCGGTGAGCGGTAGCGATGTACCATTTTCCTGGTAAACGCCCGCcaaag                                                                                                                                                                                                        >  1:1303941/1‑237 (MQ=255)                                          ttgtgggggccACAAACCAAGGATACCTGTTGCAGCCGATTAACGATGTTTGCTGGGAGGTCGCGAAAAAAAG‑GGAGCCGGGTGCCAAAATCCAACAGAGGAAAGCGCG‑AAATATCAAGACCGGCGGTTAACGCCGAGAAGTCAAC‑TAATTTGCTTCC‑CAGCGGGTG‑GGGGGTTTGCGCACCGTCGGCCGGCGGTGACCGATAGCAATGTACCATTTCACTAATAACCGCCAGCAAAg                                                                                                                                                                                                         <  1:1346567/229‑1 (MQ=255)                                              ggTGCCCACAAACC‑AGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAAACCCGCGAAGACGAA‑TAATTTGCTTTC‑CAGCGTGTC‑GAGGGCTTGCGCAACGCCGGCCCGCGGTGAGGGGTAACGATGGTCCAATTCACTGGTAACCGCCCCCAAAGGAACt                                                                                                                                                                                                    >  1:29977/1‑238 (MQ=255)                                                tGCCCACAAACC‑AGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACACCGCGAAGTCGAC‑TAATTTGCTTTC‑CCGCGCGTC‑GAGGGCTTGCGCCACCGCGGCCTGCGGGGGGCGGTAGGGATGTACCCTTTTCCCGATAAACGCCCGCAAAGGGACTTc                                                                                                                                                                                                  >  1:985742/1‑238 (MQ=255)                                                 gCCCAAAACCC‑AGGATTCCTGTTGCAGACGCTTAACAATGGTTTCTTGTACTTCGCGAAAAAACC‑GGCGACGGGTGCCAAAATCCATCAGAGGGAAGCGCG‑ACATATCAAGCCCGGCGGTTAACGCCGAGAAGTCGAC‑TAATTTGCTTTC‑CAGCGTGTC‑GAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTCCCATTTCACTGATAACCGCCAGCAAAGGAACTTcc                                                                                                                                                                                                 <  2:486438/238‑1 (MQ=255)                                                 ccccACAAACC‑AGGATTCCTGTGGCACACGCTGAACGATGGTTTCGTGTAGTGCGCGAGAAAAAC‑GGCGACGGGTGCCAAAACCCAACAGATGGAAGCGCG‑ACATATCAAGCCCGGCGGTTAACGCCGAGAGGTCGAC‑TAATTTGCTTCC‑CAGCTTGTC‑GAGGGCTTGCGCACCGGCGGCCTGCGGTGCGCGATAGCGATGTACCATTTCACTGATAGCCGCCAGCAAAGGAACTTcc                                                                                                                                                                                                 <  1:351736/237‑1 (MQ=255)                                                  cccACAAACC‑AGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAAAGCCCGGAAGTCGAC‑TAATTTTCTTTC‑CAGCCTGGC‑GGGGGCTTGCGCAACGTCGGCCCGCGGGGAGCGGTAGCGGTGGACCATTTTCCTGGTAAACGCCCGCCAAGGAACTTCCCa                                                                                                                                                                                               >  1:1403591/1‑239 (MQ=255)                                                  cccAAAACCA‑AGGATCCCTGTTGGAGAGGTTAAACGAGGGTTTTTTGAACGTGGGGAGAAAAAC‑GGCGAGGGGTGCAAAAATCCATCAGATGGAGGCGCG‑ACATATCAAGCCCGGCGTTTAACGCGGAGAAGTCGAC‑TAATTTGCTTCC‑CGGCGTGTG‑GAGGGCTTGCGCAACGTCGGCCTGCGGGGAGCGATAGCGATGTACCTTTTCACTGATAACCGCCAGCAAAGGAACTTCCCa                                                                                                                                                                                               <  1:560384/239‑1 (MQ=255)                                                       aaaGC‑AGGATTGCGGTGGCAGACGCTTAACGGTGGTTTCTTGAAGTCGGAGAAAAAAAC‑GGCGACGGGTCCAAAAACCCATCAGGTGGAGGCGCG‑ACAGATCAAGACCGGCGGTGAACGCCGAGAGGGCGCC‑TAATTTGCTTTC‑CAGCGTGTC‑GGGGGTTGGCGAAGCGCCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGa                                                                                                                                                                                          <  1:528765/239‑1 (MQ=255)                                                         aCC‑AGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGAC‑TAATTTGCTTTC‑CAGCGTGTC‑GAGGGCTTGCGCAACGTCGGCCTGCGGTGagcgatag                                                                                                                                                                                                                                           <  2:527155/188‑1 (MQ=255)                                                          cc‑AGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACGCCGAGAAATCGAC‑TAATTTGCTTTC‑CAGCGTGTC‑GAGGGCTTGGGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCCTTTCACTGATAACCGCCAGCAAAGGAACTTCCCCGAGGAT‑Ga                                                                                                                                                                                      >  2:1193117/1‑239 (MQ=255)                                                                    cTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAATGCCGAGAAAGCGCT‑CAATTTTGTTTCTAACCGGGTC‑GGGGGC‑TGCCCAACGTCCGCCCGCGGTGGGCGCTACAGATTTAACCTTTCACTTCTAACAGCCAACCACGGGACTTCCCCAAGCAT‑GGCATCAccg                                                                                                                                                                              >  1:1065006/1‑236 (MQ=255)                                                                    cTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACGCCGCGAAGGCGAC‑TAATTTTCTTTC‑CAACGTGTC‑GGGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGCTAGCGATGGACCATTTCACTGCTAACCGCCAGCAAAGGAACTTCCCCGAGGAA‑GACGTCAccc                                                                                                                                                                              >  1:804138/1‑236 (MQ=255)                                                                    cTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGAC‑TAATTTGCTTTC‑CAGCGTGTC‑GAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCCGCAAAGGAACTTCCCAGAGGGT‑GACtttccgc                                                                                                                                                                              >  2:440769/1‑233 (MQ=255)                                                                    cTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAAACACGAGAAGTCGGA‑TAATTTTTTTTC‑CCGCGTGTT‑GAGGGCCTTCGCAAAACCGGGCCGCCGTGAGAGATATGGATGTACCAATTTACTAGACACCCCCCGCAAAAGAACTTCCCCGAGGAT‑GACTTCAAGc                                                                                                                                                                              >  1:260474/1‑238 (MQ=255)                                                                          cAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAAACCCGGGCAGTCGGC‑TAATTTTCTTTC‑CAGCGGGGC‑GGGGGGCTGCGCAACGTCGGCCTGCCGTGGGCGGTAGCGATGTACCATTTTCCTGATAACCGCCCGCAAAGGAACCTCCCCGAGGGG‑GACTTTACGCCACagg                                                                                                                                                                        >  1:1631363/1‑238 (MQ=255)                                                                                         ggTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAAAGCCGAGAAGTCGAC‑TAATTTGCTTTT‑CAGCGGGTC‑GGGGGCTTGCGCAACGTCGGCCTGCGGGGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GGCTTCACGCCACGGGCCGC‑TTTAACGAata                                                                                                                                                        >  2:1219904/1‑238 (MQ=255)                                                                                           tttCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGAC‑TAATTTGCTTTT‑CAGGGGGTC‑GAGGGCCTGCGCAACGTCGGCCTGCGGCGGGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCagag                                                                                                                                                                                            >  2:926235/1‑202 (MQ=255)                                                                                             ttttGTACTTCGGGAGAAAAAC‑GGCGACGGGTGCCAAAACCCAACAGATGGAAGCGCG‑ACATATAAAGACGGGCGGTTAACGCCGAGAAGTCGAC‑TAATTTGCTGTC‑CGGCGTGTG‑AAGGGCTTGCGCAACGCCGCCCGGGGGTGAGCGATAGCGATGTACCATTTATCTGAAAACCGCCAGCAAAGGAACTTCCCa                                                                                                                                                                                               <  1:84394/195‑1 (MQ=255)                                                                                             tCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGAC‑TAATTTGCTTTC‑CAGCGTGTC‑GAGGGCTTGCGCAACGTCGGCCTGCGGTGagcga                                                                                                                                                                                                                                              <  2:65129/150‑1 (MQ=255)                                                                                               ttGTACTTCGCGAGAAAACC‑GGGTCCGGGTCCAAAAACCCATCGGA‑GGAAGCCCGAACATATCAAGCCCGGCGTTTAACGCAGAGAGTGAGAT‑TAATTTGTTTCC‑CGGCGGGTG‑GAGGGCTTGCAAAAGTGGGGTTTGCGGTGGGATATGGGGTTTCACCTTTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCt                                                                                                                                                 <  1:1672768/239‑1 (MQ=255)                                                                                                    cTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGGTAACGCCGAGAAGTCGAC‑TAATTTGCTTTC‑CAGCGTGTC‑GAGGGCTTGCGCAACGTCGGCCTGCGGGGGGCGATAGCGATGTTCCATTTCACTGATAACCGCCCGCCAAAGAACTTCCCAGAGGAT‑GAATTCCAGCCACGGGCCGC‑TTAAACGAAAATCCCGCTTGcta                                                                                                                                            >  1:1569463/1‑237 (MQ=255)                                                                                                    cTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGATAACGCCGAGAAGTCGAC‑CAATTTGCTTTC‑CAACGGGTC‑GGAGGCTTGCGCAACGTCCGCCCGCGGTGAGCGATAGCGATGTACCCTTTTACTGATAAACGCCCGCAAAGGAACTCTCCACAGGAT‑GGCTTCACGCCACGGGGCGC‑ATAAAAGAATATCCCGCCTTCCa                                                                                                                                            >  1:200225/1‑239 (MQ=255)                                                                                                      tCGGGAAAAAAACGGGCGACGGGTGCCAAAACCCATCAGATGGAGGCGCG‑AAATATCAAGCCGGGCGTTTAACGCCGAGAAGTCGAC‑TAATTTGTTTCC‑CAGCGTGTC‑GAGGGTTTGCGAAACGTCGGCCTGGGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCAt                                                                                                                                           <  2:1336836/239‑1 (MQ=255)                                                                                                       cgcgAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACGCCGGGAAGTCGAC‑TAATTTGCTTTC‑CCGCGGGTC‑GAGGGCGTGCGCAACGTCGGCCCGCGGGGAGCGATAGCGATGTACCATTTTACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAA‑GACTTCACGCCACGCGACGC‑TTTAACGAATATCCAACTAGccct                                                                                                                                           >  1:881798/1‑235 (MQ=255)                                                                                                       cgcgAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACGCCGAGAAGTCCAC‑TAATTTGCTTTT‑CAGCCTGTC‑GGGGGCCTGCGCAACGTCGGCCTGCGGTGAGCGATAACGGTTGTCCATTTCCATGATAACCGCCCCCCAAGGAACTTCACCGAGGAA‑GACTTTACGCCACAGGGCGCTTTAAACAAattccccctttcccatt                                                                                                                                          >  1:575960/1‑224 (MQ=255)                                                                                                       cgcgAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACGCCGAGAAGGCGAC‑TTATTTGCTTTT‑CCGCGGGTC‑GGGGGGTTGCGCAACGTCGGGCCGCGGTGAGCGATAGCGGTGTACCATTTCACTGTTAACCCCCCGCAAAGGAACTTccccaagggaggcc                                                                                                                                                                                     >  2:84394/1‑186 (MQ=255)                                                                                                       cgcgAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACGCCGAGAAGGCGAC‑TAATTTGCTTTC‑CAGCGGGTC‑GAGGGCTTGCGCAACGTCGGCCTGCGGGGAGCGATAGCGATGTAACATTTCACTGATAACCGCCAGCCAAAGAAATTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAACTTGCCATTa                                                                                                                                         >  2:1572607/1‑239 (MQ=255)                                                                                                       cgcgAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACGCCCAGAAGTCGAC‑TAATTTGCTTTC‑CAGCGCGGC‑GAGGGCTTGCGCAACGTCGGCCTGCGGGGAGCGATAGCGGTGTa                                                                                                                                                                                                                                    >  1:65129/1‑150 (MQ=255)                                                                                                       cgcgAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACAGCGAAAAAACGAA‑TAAATTTCTTTC‑CAGAGGGTC‑GAGGGGTTGCCCAAAGACCGCCCCCGGGGGGCCGTAAGGAGGTACCATTTCTACGATAAACGCCCGCAAAAGAAATTCCCAGAGGATGGACTT‑ACACCCCCGGGCGC‑TTTAAAGAAAAATCACCTTGCCtttt                                                                                                                                         >  1:1517267/1‑238 (MQ=255)                                                                                                         cgAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAAAGCCGAGAAGTCGAC‑TAATTTGCTTTC‑CAGCGTGTC‑GAGGGCTTGCGCAACGTCGGCCTGCGGGGAGGGATAGCGATGTACCCTTTTACTTATAAACGCCCGCCAAGGAACTTCCCAGAGGAT‑GACTTCACGCCCCCGGGCGC‑TTAAAAGAAAATCCAGCTTTCCATTATc                                                                                                                                       >  2:1616491/1‑239 (MQ=255)                                                                                                              aaaaC‑GGCGAGGGGTCACAAAACCCAACAGAGGGAAGCGGA‑AAATAACAAGCCGGGGGTTTAACGCCGAGAGGGCGAC‑TAATTTGTTTTC‑CGGCGTGGG‑GGGGGTTTGCGAACCGTCGGCCTGGGGGGGGCGATAGCGATGTACATTTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTgg                                                                                                                                  <  1:901097/239‑1 (MQ=255)                                                                                                                                aaaaaTCCATCAGATGGAGGCGCT‑ACATACCAAGACCGGGGGTTAACCCCGAAAAGTCGAC‑TAATTTGCTTTC‑CAGCGTGTC‑GAGGGCTTGCGCAACGTCGGCCTGCGGTGGGCGATAGCGATGTCCCTTTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTc                                                                                                                  <  2:1631363/237‑1 (MQ=255)                                                                                                                                    atcaaccgtaTGGAGGCGCG‑AAATACCAGGCCCGGGGGTAAACCCGGGAAAGTCGAC‑AAATTTGTTTTC‑CAGCGTGTC‑GAGGTCTTCCCCAACTGCGGCCGGGGGGGGGCGATAGCGATGTACCTTTTCCCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTTAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGt                                                                                                             <  1:1219904/230‑1 (MQ=255)                                                                                                                                                  aGCGCG‑ACATATCAAGACCGGCGGTTAAAGCCGCGAAGTCGAC‑TAAATTGCTTTC‑CAGCGTGTC‑GAGGGCGTTCGCAACGGCGGCCTGCGGTGAGAGATAGCGATGTACCATTTCACTGTTAACCGCCCGCCAAGGAAATTTCCCGAGGAT‑GGCTTCCCCCCCCGGGGCGC‑TTAAAAGCATATCCACATTGCCCTTATCTTTTGACACGGTGACTTGTTTCGGGGTAAAACgggaatc                                                                                                >  1:1041501/1‑233 (MQ=255)                                                                                                                                                       cgacaatacacgACGGGCGTTTAACGCCAGGAAGTCGAC‑TAATTGGCTTTC‑CGGGTGGTG‑GAGGGCTTGCAAAACGTCGGCCGGCGGTGAGAGATGGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGt                                                                                            <  1:1083482/227‑1 (MQ=255)                                                                                                                                                         aaatatCAAGCCGGGCGGTTAACGCCGAGAAGCCGAC‑TAATTTCCTTCC‑CGGCGTGTC‑GGGGGCTTCCGCAAGGTCGGCCGGGGGTGAGCGATAGCGATGTACCATTTCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                          <  1:1705436/236‑1 (MQ=255)                                                                                                                                                         aaaagtCAAGCCCGGGGGTTAACGCCGAAAGGTCGCC‑TAATTTGCTTCC‑CGGCGTGTC‑GAGGGCTTGCGCAACGTCGGCCTGCGGGGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                          <  1:1388069/233‑1 (MQ=255)                                                                                                                                                         aCATAACAAGCCGGGCGGTTAACGCCGAGAAGTCGAC‑TAATTGGCTTCC‑CAGGGTGGC‑GAGGGCTTGCGCAACGTCGGCCTGGGGGGAGCGATGGCGATGTCCCTTTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                          <  2:555342/238‑1 (MQ=255)                                                                                                                                                          cATATCAAGCCCGGCGGTTAACGCCGAGAAGTCGAC‑TAATTTGCTTTC‑CAGCGTGTC‑GAGGGCTTGCGAAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAAcc                                                                                        <  2:1446869/239‑1 (MQ=255)                                                                                                                                                            tatCAAGCCCGGCGGTTAACGCCGAGAAGTCGAC‑TAATTTGCTTTC‑CGGCGTGTC‑GGGGGCTTGCGAAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAg                                                                                      <  2:1214360/239‑1 (MQ=255)                                                                                                                                                             taagacgcgggggggtAACGCGGAGATGGCGTC‑TATTTTGTTTTC‑CGGTGTTTC‑GAGGGCTTCCGCACCGTGGGCCGCTGGGGGGCGATAGCGATGTACGATTTCACTGAAACCCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGt                                                                                     <  1:403938/224‑1 (MQ=255)                                                                                                                                                             atCAAGACCGGCGGTTAACGGCGAGAAGCCGAC‑TAATTTGCTTCC‑CGGCGTGTC‑GAGGGCTTGCACAACGTCGGCCTGCGGTGAGCAATAGCGATGTACCATTTCACTAATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGt                                                                                     <  2:1053913/239‑1 (MQ=255)                                                                                                                                                                       ggggTAAACGCCGAGAAGGGGAC‑TAATTGTCTTTC‑CAGCGTGTG‑GGGGGCTGGCGCAACGTCGGCTGGCGGTGCGCAATACCGATGTCCCTTTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                                                                            <  1:1071719/236‑1 (MQ=255)                                                                                                                                                                       gcggTTTACGCCCGGAAGTCGAC‑TAATTTTCTTTC‑CCGCGTGTC‑GAGGGCTTGCGCAACGTCGGCCTGCGGTGGGCGGTAGCGATGTACCATTTTACCGGTAACCCCCCGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAAAATTCCGCTTGCCCTTATCGTTGGACACCGGGACTTGTTCCGGGGTAAAGCGGGACTCGCCGAAACAGTTAAGATAGTc                                                                           >  1:814385/1‑239 (MQ=255)                                                                                                                                                                       gcggTTAACGCCGAGAAGTCGAC‑TAATTTGCTTTC‑CAGCGGGTC‑GCGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGGTGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCCTTATCGTTGGACACGGTGACTTGTTCCGGGGTTAAGCGGGACTCGCGGAACCAGTTAAAATAGTc                                                                           >  1:313201/1‑239 (MQ=255)                                                                                                                                                                       gcggTGAACGCCGAGAAGTCGAC‑TAATTTGCTTTG‑CAGCGTGTC‑GAGGGCTTGCGCAACGCCGGCCTGCGGTGGGCGATAGCGATGTACCATTTAACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                                                                            <  2:647043/238‑1 (MQ=255)                                                                                                                                                                        cggTTAACGCCGAGAAGTCGAC‑TAATTTGCTTTC‑CAGCGTGTC‑GGGGGGTTGCGCAAAGTCGGCCTGCGGTGGGCGCTAGCGATGTACCATTTTACTGGTAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCCCGGGCCGC‑CTAAACGAATATCCCGCTTGCCATTATTGTTGGACACGGGGGCTTGTTCCGGGGTAAAGCGGAACTCGCGTAACCAGCTAAGATAGtgc                                                                          >  1:1723530/1‑237 (MQ=255)                                                                                                                                                                            taccGCCGAGAA‑TCGACTTAATTTGCTTCC‑CGTGGTGTC‑GAGGGCTGGCGCAACGTCGGCCTGCGGTGAGCGATGGCGATGTCCCATTCCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                                                                      <  2:1350585/236‑1 (MQ=255)                                                                                                                                                                                  ggagaAGCCGAC‑AAAGTTGGTGCC‑AAGGGTTGC‑GAGGGCGTGCGAAGCGTCGGACGGGGGTGAGCGATAGGGATGTCCCATTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTaaag                                                                 <  2:1539117/237‑1 (MQ=255)                                                                                                                                                                                      aaGTCGTC‑TATTTTGCTTTC‑CGGCGTTTC‑GGGGGCTTGCGCAACTCCGGCCGGCGGTGGGCGATACCAAGTTACCATTCCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAgg                                                            <  2:635591/239‑1 (MQ=255)                                                                                                                                                                                       aGTCGAC‑TAATTTGTTTTC‑CAGTGTGTG‑GAGTGTTTGCGCAACGCCGGCCGGCGGTGAGGGATAGCGATGTCCCATTTCCTTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAgg                                                            <  2:603759/238‑1 (MQ=255)                                                                                                                                                                                                 aTTTGCTTTC‑CAGCGTGTC‑GAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGCTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCCGCCTTAAAGAAAGGCAGGGCAGaa                                                  >  2:329497/1‑239 (MQ=255)                                                                                                                                                                                                       tttC‑CAGCGTGTC‑GAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTCCCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCa                                             <  2:1403591/238‑1 (MQ=255)                                                                                                                                                                                                       ttcc‑cAGCGTGTC‑GAGGTCTTGCGCACCGTCGGCTGGCGGTGAGCGATAGCGATGTACCATTTCACGAATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCa                                             <  2:1260684/235‑1 (MQ=255)                                                                                                                                                                                                       attccccgcgttgc‑GAGGGGTTGCAGAGCGCCGGGCTGCGGTGAGCGATAGCAGTGTACCATTTAACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCa                                             <  1:195852/226‑1 (MQ=255)                                                                                                                                                                                                          tcaggtgtggC‑GGGGCCTCGCGCAACGCGGTCCTGAGGTGGGCGATAGCTAGTTACCATTTCCCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTg                                          <  1:1193117/233‑1 (MQ=255)                                                                                                                                                                                                            gtaaaagagacgggcgtggcccAAGGTCGGCCGGCGGTGGGAAATGGCGTTGTCCCATTCCATTGATACCCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTg                                          <  1:1639484/217‑1 (MQ=255)                                                                                                                                                                                                                gtgtG‑GAGGGCTTGGGCAACGTCGGCCGGGGGTGAGCGAAAGCAATGTACCATTACCTTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATAtt                                     <  2:295244/238‑1 (MQ=255)                                                                                                                                                                                                                 tgtC‑GAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATtca                                   >  1:973079/1‑239 (MQ=255)                                                                                                                                                                                                                 tgtC‑GAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATtca                                   >  2:1671242/1‑239 (MQ=255)                                                                                                                                                                                                                  gtC‑GAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATtca                                   >  2:638056/1‑238 (MQ=255)                                                                                                                                                                                                                     cgggggCTTGCGCAACGTCGGCCTGGGGGGAGCGATAGCGATGTACCATTTCACGAATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTCGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATtcatca                                <  2:575960/236‑1 (MQ=255)                                                                                                                                                                                                                         ggCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCACAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTcatcat                               >  2:1630027/1‑236 (MQ=255)                                                                                                                                                                                                                           cTTGCGCAACTGCGGCCTGGGGTGAGCGATGGCGATATCCCATTTCACAGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTg                           <  2:979593/238‑1 (MQ=255)                                                                                                                                                                                                                           cTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGc                          >  2:1373169/1‑239 (MQ=255)                                                                                                                                                                                                                            ttGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCTGCCTTAAAGAAAGACAGGGCAGAAAGCCACTGATATTCATCATCCTga                          >  1:1165731/1‑237 (MQ=255)                                                                                                                                                                                                                            ttGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCa                         >  1:916604/1‑239 (MQ=255)                                                                                                                                                                                                                             tGCGCAACGTCGGACTGCGGTGAGCGATAGCGTTGTACCATTTCCCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGGCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATCGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAg                        >  1:194000/1‑239 (MQ=255)                                                                                                                                                                                                                              gcgcAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAg                        >  2:890947/1‑238 (MQ=255)                                                                                                                                                                                                                                 cAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGgcgc                    <  2:916604/239‑1 (MQ=255)                                                                                                                                                                                                                                  aaCGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTCCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGgcgc                    <  2:1723530/238‑1 (MQ=255)                                                                                                                                                                                                                                    cGTCGGCCTGCGGTGAGCGATAGCGATGTCCCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACggg                                                                                                                                                                        <  2:1357864/89‑1 (MQ=255)                                                                                                                                                                                                                                    cGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACggg                                                                                                                                                                        >  1:1357864/1‑89 (MQ=255)                                                                                                                                                                                                                                    cGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATAtt                                     >  2:1357041/1‑219 (MQ=255)                                                                                                                                                                                                                                    cGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATtcatca                                >  2:1639484/1‑224 (MQ=255)                                                                                                                                                                                                                                    cGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAgg                 >  2:742233/1‑239 (MQ=255)                                                                                                                                                                                                                                     gcgggCCTGGGGTGGGCGATAGCGATGTCCCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGt                <  1:1373169/236‑1 (MQ=255)                                                                                                                                                                                                                                           cggCGGTGAGCAATAGCGATGTACCATTTCCCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCa          <  2:985742/237‑1 (MQ=255)                                                                                                                                                                                                                                           cTGGGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCa          <  2:200225/239‑1 (MQ=255)                                                                                                                                                                                                                                           cTGGGGGGAGCGATAGCGAGGTACCTTTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCa          <  1:1079168/239‑1 (MQ=255)                                                                                                                                                                                                                                              cGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGCGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATc        >  1:752984/1‑238 (MQ=255)                                                                                                                                                                                                                                                   agcgatagcgatGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTGa  >  2:233957/1‑239 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 TCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACC‑AGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAAC‑GGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCG‑ACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGAC‑TAATTTGCTTTC‑CAGCGTGTC‑GAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGAT‑GACTTCACGCCACGGGCCGC‑TTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTGA  >  NC_000913/989709‑990183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑