Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,826,442 A→G 12.0% E18G (GAG→GGG)  srlE → glucitol/sorbitol‑specific enzyme IIB component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,826,4420AG12.0% 278.5 / 2.0 104E18G (GAG→GGG) srlEglucitol/sorbitol‑specific enzyme IIB component of PTS
Reads supporting (aligned to +/- strand):  ref base A (64/24);  new base G (2/13);  total (66/38)
Fisher's exact test for biased strand distribution p-value = 1.97e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

TCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCT‑GCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCC  >  NC_000913/2826204‑2826677
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             
tCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCT‑GCCACTTGGCCCACTGGCGGTGAGGTATCTGCTGGGTGGTCTGGGCACCAAATTATTCCGCGGCTGGGGGACCGATCTGAACACCGCCACTTTTG‑AGAAAAAGGTGGGCCTTCAACTTGGACAAAAA‑AT‑TCACCTGGCAGGAA‑CAACATCA‑TGAAG‑CATATTCGGATCGAAAAAGGAAA‑GGGTGGCT‑GGGGCGGCCC‑GCTTGa                                                                                                                                                                                                                                               >  1:588910/1‑239 (MQ=255)
 cTTGGCATTGCCAGCGGTCTGACAACGCTGAACCT‑GCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAg                                                                                                                                                                                                                                              <  2:1175302/239‑1 (MQ=255)
  ttGGCATTGCCAGCGGTCTGACAACGCTGAACCT‑GCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCG‑TCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGa                                                                                                                                                                                                                                               >  2:647352/1‑236 (MQ=255)
    ggCATTGCCAGCGGTCTGACAACGCTGAACCT‑GCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTTACCAATTTCTTCCGCGGCTGGGGGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCCTTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATAGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑g                                                                                                                                                                                                                                          >  1:1377968/1‑239 (MQ=255)
     gCATTGCCAGCGGTCTGACAACGCTGAACCT‑GCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGGTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CCACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAA‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑Ga                                                                                                                                                                                                                                         >  2:1464146/1‑239 (MQ=255)
     gCATGGCCGGGGTTCTGCCAACGCGGAACCT‑GCCACTGGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCCCCAATTTCTTCCGCGGCTGGGGGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑g                                                                                                                                                                                                                                          <  1:1464146/238‑1 (MQ=255)
           ccAGCGGTCTGACAACGCTGAACCT‑GCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGGTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGGGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGGCC‑GCTTGAGCT‑GAAAGcc                                                                                                                                                                                                                                    >  2:143768/1‑238 (MQ=255)
               cGGTCTGACAACGCTGAACCT‑GCCACTTGGCCCACTGGCGGTGAGTTTTCTGCCGGGTGGTCTGGTCACCAATTTCTTCCCCGGCTGGGTGACCGATCTGACCACCGCCCTTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑AT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTTCGATCGACAAAAGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCAccc                                                                                                                                                                                                                                >  1:294312/1‑236 (MQ=255)
                     gACAACGCTGAACCT‑GCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGaaa                                                                                                                                                                                                                         >  2:1493061/1‑239 (MQ=255)
                      aCAACGCTGAACCT‑GCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGGCACCAATTTCTTCCGCCGCTGGGTGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TTACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGGAC‑GGGGGGCT‑GGGGCGGCCC‑GCTTTGGCT‑GAAAGCCACGCCGGGaaaa                                                                                                                                                                                                                        >  2:863399/1‑239 (MQ=255)
                         agggagacccT‑CCCACTGGCCCCACGGGGGGTGATTTATTTGCTGGTTGGTCTGGTCACCATTTTCTCCCCCGGCTGGGTACCCAATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGaaaaaaa                                                                                                                                                                                                                     <  2:858754/231‑1 (MQ=255)
                         aCGCTGACCCT‑GCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGaaaaaaa                                                                                                                                                                                                                     <  2:1492089/239‑1 (MQ=255)
                          cGCTGAACCT‑GCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGCCCGCCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGaaaaaaaa                                                                                                                                                                                                                    <  2:893403/239‑1 (MQ=255)
                           ccTGAACCCGGCCACTTGGCCACCGGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTCCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGaaaaaaaa                                                                                                                                                                                                                    <  2:243252/238‑1 (MQ=255)
                            cTGAACCT‑GCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGGTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTG‑TGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCTGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGaaaaaaaaa                                                                                                                                                                                                                   >  2:857793/1‑237 (MQ=255)
                             ctcacccggCCACTTGGCCCACGGGCGGGGATTTATCTGCTGGTTGGTCTGGCCACCAATTTCTTCCGCGGCGGGGTGACCGATCTGCCCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATc                                                                                                                                                                                                                  <  1:308183/231‑1 (MQ=255)
                              gAACCT‑GCCACTTGGCCCACTGGCGGTGAGGTATCTGCTGGTTGGTCTGGGCACCAATTTCTTCCGCGGCCGGGGGACCGATCTGACCACCGCCCTTTTTG‑AGAAAAAGATGGGCATTTAACTTTCACAAAAA‑GG‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CCTATTCGGATAGAAAAAGGAAA‑GGGTGGCT‑GGGGCGGCCC‑GCCTGAGCT‑GAAAACCACGTCGGGAAAAAAAATC‑g                                                                                                                                                                                                                >  1:1679626/1‑238 (MQ=255)
                               acccT‑GCCCCTGGGCCCCTGGGGGGTGAGTTATGTGCGGGTGGGTTTGGTCACAAATTTCTCCCGGGGCGGGGTGACCGTTCGGACCACCGCCATTTTTG‑AGAAAAAGATGGGAATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑Gt                                                                                                                                                                                                               <  1:857793/236‑1 (MQ=255)
                               aaCCT‑GCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GGAAACCACGCCCGGAAAAAAAATC‑g                                                                                                                                                                                                                >  2:1064771/1‑237 (MQ=255)
                                     gCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGGGACCGATCTGACCACCGCCCTTTTTG‑AGAAAAAAATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGGGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAAAC‑GTCtata                                                                                                                                                                                                          >  2:863067/1‑238 (MQ=255)
                                                               aTCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGAttg                                                                                                                                                                               >  1:234389/1‑239 (MQ=255)
                                                                tCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGAttgt                                                                                                                                                                              >  2:1337998/1‑239 (MQ=255)
                                                                     tggttggtCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACa                                                                                                                                                                         >  2:1594710/1‑239 (MQ=255)
                                                                     tggttggtCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGACGGGAAAAAACATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGACTGTTGACa                                                                                                                                                                         >  1:1601214/1‑239 (MQ=255)
                                                                       gttggtCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATc                                                                                                                                                                                                                  >  1:182026/1‑197 (MQ=255)
                                                                       gttggtCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATc                                                                                                                                                                                                                  <  2:182026/197‑1 (MQ=255)
                                                                       gttggtCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACaa                                                                                                                                                                        >  2:603286/1‑238 (MQ=255)
                                                                       gttggtCTGGTCACCAATTTCTTACGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACaa                                                                                                                                                                        >  2:542125/1‑238 (MQ=255)
                                                                          ggtctggtcACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTg                                                                                                                                                                    >  2:156956/1‑239 (MQ=255)
                                                                            tctggtcACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGc                                                                                                                                                                  >  2:553865/1‑239 (MQ=255)
                                                                              tggtcACCAATTTCTTCCGCGGCTGGGTGACCGAGCTGACCACCGCCATTTTTG‑AGTAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACATGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCGACGCCGGGAAAAAAAATC‑GGCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGaac                                                                                                                                                                >  1:245485/1‑239 (MQ=255)
                                                                                           cttcCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTActggctg                                                                                                                                                   >  2:1605452/1‑239 (MQ=255)
                                                                                             tcccgcggggggagacacaatcgACCACCCGCATTTTTGAAAAAAAAGAGGGGAATAAAACTAGAAAAAAAA‑GT‑CACCCTGGAGGGAG‑CAACATCA‑GCACG‑AATTTTCGGATGAAAAAGGGAAG‑GGGGGGGG‑GGGGGGGCCC‑GCTGGAGCG‑GAAAGCCACGCCGGAAAAAAAAATC‑GTCTATACCGCCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTActggctgg                                                                                                                                                  <  1:603286/217‑1 (MQ=255)
                                                                                               cgcgGCTGGGTGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAg                                                                                                                                               >  2:385302/1‑239 (MQ=255)
                                                                                               cgcgGCTGGGTGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAg                                                                                                                                               >  2:1445473/1‑239 (MQ=255)
                                                                                                      gggTGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTg                                                                                                                                        >  2:451533/1‑239 (MQ=255)
                                                                                                        gtcccctctccaccccccccctgtttgG‑AAAAAAAGATGGAC‑TTACACTTAAAAAAAAAGGT‑CCACGGGGCAGGAA‑CAGCATCA‑TGACA‑AATATTGGGACGAAAAAAGGAAG‑GGGGGGCG‑GGGGCGGCCC‑GCTTGAGAG‑AAAAGCCACGCCGAAAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGa                                                                                                                                       <  1:470575/215‑1 (MQ=255)
                                                                                                        gcgaccattacaacccccGCTATTTTTG‑AAAAAAAGTTGACCA‑TCCACTTGTAAAAAAA‑GTGCCGCCTGGAAGGAG‑C‑ACGTCACTGTCT‑AATTTTTGGTCCGAAAAAGGAAC‑GGGGGCTT‑GGGGCGGCCC‑GCTGAACTT‑GAAACCCACGCCGGAAAAAAAAATC‑TTTTATACCACCGCCGTTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGa                                                                                                                                       <  1:1285625/226‑1 (MQ=255)
                                                                                                         aaaagaatcagccacccGCAATTTGTG‑AAAAAAAGATGGAATTTCAACTAAAACAAAAA‑GT‑GCCCCGGGGAGGAG‑AAACATAA‑TCCCC‑TATATTGGGATCG‑AAAAGGAACGGGGGGGCG‑GGGGCGGCCC‑CCTTAAGTT‑AAAGGCCACGCGGGAAAAAAAAATC‑TTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGa                                                                                                                                       <  1:815849/222‑1 (MQ=255)
                                                                                                              gATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGAttt                                                                                                                                >  2:1401585/1‑239 (MQ=255)
                                                                                                              gATCTAACCACCGCCATCTTTG‑AGAAAAAGATGGGCATTCAAATTGTACAAAAA‑GA‑TCACCTGGCAGTAG‑CAACATCT‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGGGGCT‑GGGGCGGCAC‑GGGTGGGCT‑GAGAGCCACGCCCGGAAATAAACTC‑GTTTACATCTCCGCCGGTACCCGGGCTGCGATGGTTGACAAACTGGCACACGTTACTGGCAGGCAGGCTATTGAAggtatt                                                                                                                                >  1:934053/1‑235 (MQ=255)
                                                                                                               aTCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTa                                                                                                                               >  2:59068/1‑239 (MQ=255)
                                                                                                                 cTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTaaa                                                                                                                             >  2:808023/1‑239 (MQ=255)
                                                                                                                         cGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCCGGCTATTGACGGGTTTAAAGAAGGGGa                                                                                                                     >  1:1357978/1‑239 (MQ=255)
                                                                                                                         cGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTg                                                                                                                                        >  2:1416824/1‑220 (MQ=255)
                                                                                                                         cGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGAGGGATTTaacacaggaga                                                                                                                     >  1:518550/1‑230 (MQ=255)
                                                                                                                         cGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTaaa                                                                                                                             >  2:815849/1‑231 (MQ=255)
                                                                                                                         cGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGa                                                                                                                     >  2:1509798/1‑239 (MQ=255)
                                                                                                                              tttttG‑AGAAAAAGATGGGCATTCAACTTGCACAAAAA‑GA‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGCAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGCAGGTGAcccc                                                                                                                 >  1:1083733/1‑238 (MQ=255)
                                                                                                                              tttttG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGGTTTAAAGAAGGTGAAccc                                                                                                                 >  2:1580126/1‑238 (MQ=255)
                                                                                                                              tttttG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAAccc                                                                                                                 >  2:1093130/1‑238 (MQ=255)
                                                                                                                              tttttG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAAccc                                                                                                                 >  2:846168/1‑238 (MQ=255)
                                                                                                                                                ggCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGgt                                                                                               >  2:1726929/1‑239 (MQ=255)
                                                                                                                                                    ttaaatatGAAAAAAAG‑GT‑TCACCGGGCAGGAG‑AAACATAG‑TGCGG‑CAGTTGGGGCAGAAAAAAGGACG‑GGGTGGCG‑GGGGCGCCCC‑GCTGGAGAG‑AAAACCCCCGCGGGAAAAAAAAATC‑GTCTATCCCCCCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTcg                                                                                           <  1:863399/232‑1 (MQ=255)
                                                                                                                                                    ttaaaCTTAAAAAAAAA‑GT‑TCACCGGGCAGGAG‑CAAAATCA‑TGACG‑CATATTCGGATCGAAAAAGGAGG‑GGGGGGCG‑GGGGCGGCCC‑GTTTGAGCA‑GAACGCCACGCCGGAAAAAAAAACC‑GTTTATACCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTcg                                                                                           <  1:1600494/236‑1 (MQ=255)
                                                                                                                                                    tgcaccTTAAACAAAAA‑GT‑CAACCGGGCAGGAG‑AAATGAC‑‑TGAAGACATTATCGGAGAAAAAAAGGAGC‑GGGTGGAT‑GGGGGGGCCG‑GGTGGAGCT‑AAAACCCACCCCGGGAAAAAAAATC‑CGCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTcg                                                                                           <  1:1488417/234‑1 (MQ=255)
                                                                                                                                                    tacAACTTGAAAAAAAA‑GT‑GCACCGGGCAGGGG‑CACCCGCA‑TGACC‑CATATTCGGAAAGAAAAAGGACG‑GGGGGGGG‑GGGGCGGCCC‑GTTGGAGCT‑GAAAGCCCCGCGGGAAAAAAAAATC‑GTCTATACCCCCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTcg                                                                                           <  1:1445473/237‑1 (MQ=255)
                                                                                                                                                     ttaaactGAAAAAAAA‑AG‑TCCCCCGGGAGGAGAAAACATGA‑TGACG‑CATTTTGGGAACAAAAAAGAAAG‑GGGGGGTG‑GGGGGGGCCC‑GCTGGGGCG‑GAAAGCCACGCGGGAAAAAAAAACC‑GTCTATACCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTcg                                                                                           <  1:1493061/233‑1 (MQ=255)
                                                                                                                                                      cAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCCATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCAGCGGGCGCGGAAATTGGGGTcgcg                                                                                         >  2:1606136/1‑239 (MQ=255)
                                                                                                                                                       aaCTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCgg                                                                                        >  2:1641207/1‑239 (MQ=255)
                                                                                                                                                                         tCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTggcgg                                                                        >  2:620232/1‑239 (MQ=255)
                                                                                                                                                                         tCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTggcg                                                                         >  2:1699710/1‑238 (MQ=255)
                                                                                                                                                                         gcACCGGGCAGAGG‑CAACATCA‑GGACA‑CATATGGGGA‑CAAAAAAGGGACGGGGTGGGG‑GGGGCGGCCC‑GCTTGGGCG‑GAAAGCCACGCGGGAAAAAAAAATC‑GTCTATACCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTggcg                                                                         <  2:1357978/237‑1 (MQ=255)
                                                                                                                                                                           ccaaggCAGGAA‑CAAGAACG‑TCACG‑GATTTGGGGAACGAAAAAGGAAC‑GGGTGGCG‑GGGGCGCCCC‑CTTTGGGCG‑TAAACCCAGGGAGGGAAAAAAAATC‑GTTCAAACCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGTAAATTGGTGTCGCGGTAATCGACTGTGGCGGca                                                                      <  1:1509798/235‑1 (MQ=255)
                                                                                                                                                                                       acAACACCA‑TGACG‑AATATGCGAAACGAAAAAGGAGC‑GGGGGGGT‑GGGGAGCCCC‑GCTTGACAG‑AAAACCCACCCGGGAAAAAAAAACC‑GTCTATATCGCCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGc                                                           <  2:682325/238‑1 (MQ=255)
                                                                                                                                                                                        cAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACCCTggg                                                          >  2:673439/1‑237 (MQ=255)
                                                                                                                                                                                           catca‑tGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCa                                                       >  2:235205/1‑239 (MQ=255)
                                                                                                                                                                                              agcatgccgaatgtggggacaaaaaagGGAGG‑GGGGGGCG‑GGGGCGGCCC‑GTTTGGGCT‑GAAAGCCACGCGGGAAAAAAAAACC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCAt                                                      <  1:1331489/217‑1 (MQ=255)
                                                                                                                                                                                                 tGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTa                                                   >  2:1578144/1‑238 (MQ=255)
                                                                                                                                                                                                 tGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTa                                                   >  2:1331489/1‑238 (MQ=255)
                                                                                                                                                                                                 tGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTa                                                   >  2:176628/1‑238 (MQ=255)
                                                                                                                                                                                                   aCG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCGGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATcc                                                >  1:572947/1‑239 (MQ=255)
                                                                                                                                                                                                     acggttattgaaacaaaaaaaGAAG‑CGGTGGCTGGGGGGGGCCC‑GTTGGGGTT‑GAAAGCCCCGCGGGAAAAAAAAATC‑CTGTATATCCCCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATc                                                 <  2:1275230/223‑1 (MQ=255)
                                                                                                                                                                                                      ggagatTCAGAGCAAAAAAGGAAG‑GGGTGGCG‑GGGGGGGCCC‑GTTTGACCA‑GAAACCCACGCCGGAAAAAAAAATC‑GTCAAAATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGa                                              <  2:1565733/235‑1 (MQ=255)
                                                                                                                                                                                                       cATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGaa                                             >  1:805461/1‑239 (MQ=255)
                                                                                                                                                                                                        atttggggacaaaaaagGGAAG‑GGGGGGTG‑GGGGCGGCCC‑CCTTGGCCG‑GAAAGCCACGCCGGAAAAAAAAATC‑GTCTAAACCCCCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGaaa                                            <  1:1726929/227‑1 (MQ=255)
                                                                                                                                                                                                         tatTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAac                                           >  2:139449/1‑239 (MQ=255)
                                                                                                                                                                                                          atTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAacg                                          >  2:1575493/1‑239 (MQ=255)
                                                                                                                                                                                                          atTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAacg                                          >  1:1616049/1‑239 (MQ=255)
                                                                                                                                                                                                                   aaaaaaGGAGC‑GGGTGGCG‑GGGGCAGCCC‑GCTGGGGCA‑AAAAGCC‑CGGCGGAAAAAAAAAACGGTCTATACCCCCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTcc                                 <  1:1699710/238‑1 (MQ=255)
                                                                                                                                                                                                                   aaaaaaGGAGC‑GGGGGGTG‑GGGGGCGCCC‑ACGGGGGCT‑GAACGCCACGCCGGAAAAAAAAACC‑GTTTATACCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTcc                                 <  1:1070385/238‑1 (MQ=255)
                                                                                                                                                                                                                   aaaaaaGGAAG‑GGGGGGCG‑GGGGCGGCCC‑GCTGGCGCG‑AAACCCCCCGAGGGAAAAAAAAAGC‑GTTAACACCGCCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTAtt                                   <  1:863067/236‑1 (MQ=255)
                                                                                                                                                                                                                   aaaaaaGAACC‑GGGGGGCG‑GGGGGGGCCC‑‑CTTGGGGTGGAAACCCACGCCAGAAAAAAAAATC‑GTCAATACCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTcc                                 <  2:44505/238‑1 (MQ=255)
                                                                                                                                                                                                                    aaaaaGGGAG‑GGGTGGGTCGGGGCGGCCC‑GCGGGGGAG‑GAAGGCCCTCCCGGAAAAAAAAATA‑GAGTAAGCAACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCGTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGGGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTcc                                 <  1:620232/239‑1 (MQ=255)
                                                                                                                                                                                                                    aaaaaGGAAG‑GGGTGGCG‑GGGGCGGCCC‑GTTTGAGCT‑GAACCCCCCGCGGGAAAAAAAAACC‑GTCTATACCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTcc                                 <  2:294312/238‑1 (MQ=255)
                                                                                                                                                                                                                    aaaaaGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTccc                                >  2:889780/1‑239 (MQ=255)
                                                                                                                                                                                                                    aaaaaGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTcc                                 >  2:620560/1‑238 (MQ=255)
                                                                                                                                                                                                                     aaaaGGAGG‑GTGGGGCG‑GGGGCGGCCC‑GTTTGAGTG‑AAAACCCGCGCGGGAAAAAAAAACC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCcca                               <  2:1573267/239‑1 (MQ=255)
                                                                                                                                                                                                                     aaaaGGAGC‑GGGGGGCG‑GGCGCGTCCC‑GTTTGGGCT‑GAACCCCCCGCCGAAAAAAAAAATC‑TTTTATCCCGCCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTcc                                 <  1:1093130/237‑1 (MQ=255)
                                                                                                                                                                                                                     aaaaGGAAG‑GGGGGGCG‑GGGGCCGCCCGGCTTGGGCG‑AAGAGCGCCGCCGGAAAAAAAAAGC‑GTTAATATGCCCGCCGGGACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGGGAACCCGCGGAGGCGGAAAGTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTcc                                 <  1:602398/238‑1 (MQ=255)
                                                                                                                                                                                                                     aaaaGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACCTTACGATGCGGCATCTAACCGaacagaggtgttgcc                                >  1:44505/1‑225 (MQ=255)
                                                                                                                                                                                                                     aaaaGGAAC‑GGGTGGCG‑GGGGCGCCCG‑GCTTGAGCA‑GAAAGCCACGCGGGAAAAAAAAATC‑TTCTAAATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCcca                               <  2:200058/239‑1 (MQ=255)
                                                                                                                                                                                                                               ggtggtgt‑GGGGCGGCCG‑GATGGAGCT‑GAAAGACACACGGGGAAAAAAAACC‑GTTAATATACCCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAat                       <  2:1286991/231‑1 (MQ=255)
                                                                                                                                                                                                                               gggTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAata                      >  1:203811/1‑239 (MQ=255)
                                                                                                                                                                                                                                ggTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAata                      >  2:494663/1‑238 (MQ=255)
                                                                                                                                                                                                                                       gggggCGCCCC‑GCTGGAGCA‑GAAAGCCACGCGGAAAAAAAAAACC‑GTCTATACCCCCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACt                <  1:620560/237‑1 (MQ=255)
                                                                                                                                                                                                                                            cggcCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACgg          >  2:1194981/1‑239 (MQ=255)
                                                                                                                                                                                                                                               ccc‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCa       >  2:918002/1‑239 (MQ=255)
                                                                                                                                                                                                                                               ccc‑GCTGGGGCT‑GAAAGCAGCCCCGGAAAAAAAAAGC‑TTTTATACCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGc        <  2:529285/238‑1 (MQ=255)
                                                                                                                                                                                                                                                     ttGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCCGCGGCATCTATCCGAAACGACGTATTCCAACCCTTAATATACACTTGACGGGCAAgccc  >  1:529285/1‑239 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             
TCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCT‑GCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTG‑AGAAAAAGATGGGCATTCAACTTGAACAAAAA‑GT‑TCACCTGGCAGGAG‑CAACATCA‑TGACG‑CATATTCGGATCGAAAAAGGAAC‑GGGTGGCT‑GGGGCGGCCC‑GCTTGAGCT‑GAAAGCCACGCCGGGAAAAAAAATC‑GTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCC  >  NC_000913/2826204‑2826677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑