BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,978,164	T→A	14.5%	Y245F (TAT→TTT)	lysA ←	diaminopimelate decarboxylase, PLP‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,978,164	0	T	A	14.5%	251.1 / 6.6	113	Y245F (TAT→TTT)	lysA	diaminopimelate decarboxylase, PLP‑binding
Reads supporting (aligned to +/- strand): ref base T (47/46); new base A (2/15); total (51/62)
Fisher's exact test for biased strand distribution p-value = 3.13e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

CTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGGTGTACCAG  >  NC_000913/2977945‑2978400

cTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCACCTGAGGAATTAATACGCCAGACTGCGCTACCCGGAAGCGACCCGGGTCAATTTCCAGTTTCCCAGGGTGG‑CCCAAAT‑GGCGGGGGATTTGACCACCCGCGGCCGTCCCCCGA‑CCATAATAATTTTCGGT‑AG‑Caa                                                                                                                                                                                                                            >  1:1002320/1‑238 (MQ=255)

 tGATATGGTGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTTTTTGACGCTCCGCACCTGTGTAATTAATACGCCAGAATGCGCTACCAGGAAGGGACCCCGTTCAATTTCCAGTTTCCAAGGGTGT‑CCCAAAT‑AGCGGGCGGGTTGCTCAACCGCGGTCTTTCCCAGA‑CCATAATAAATTTCGGT‑AT‑CAAc                                                                                                                                                                                                                           >  1:1229239/1‑238 (MQ=255)

  gATATGGGGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCCCCCCATTTTTTTGACGCTCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAG‑GGCGGGCGATTTGCTCACGCGCGGCATTCCATAGA‑CCATAATAATGTTCGGT‑AT‑CAACCg                                                                                                                                                                                                                         <  2:1229239/239‑1 (MQ=255)

       ggtggtAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTTACGCCCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCCCACGCGCCGACTTCCACCAA‑CCATCATAATTGTCGGT‑AT‑CAACCGCct                                                                                                                                                                                                                      >  1:292645/1‑237 (MQ=255)

       ggtggtAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCCCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCCATTTACAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCCCctct                                                                                                                                                                                                                    >  2:265339/1‑239 (MQ=255)

        gtggtAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCACCAACCAGCACAAAGGGGCGCCTCCCCAGTTGTTGGACGCTCCGCACCTGAGTAATTAATACGCCAGACTGCGGCACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTGCGGT‑AT‑CAACCGCctct                                                                                                                                                                                                                    <  1:1085230/238‑1 (MQ=255)

          ggtAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCa                                                                                                                                                                                                                 <  2:962426/239‑1 (MQ=255)

           ttAACTACCGTACATGGCCGGGCGCATCGTAACGTCGAACCCGGCATCAACCAGCACAAAGTGGGGGCTCCCCATTTGTTTGACGCTCCGCCCCTGAGTAATTAATACGCCAGACTGCGCTCCCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCa                                                                                                                                                                                                                 <  2:340355/237‑1 (MQ=255)

           gtAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTTACGCCCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCCGTTTCACAGGGTGG‑CCCCAAT‑GGCGGGCGGTTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAAAGTTCCGT‑AT‑CCACCCCCCCTTcc                                                                                                                                                                                                                 >  1:233067/1‑237 (MQ=255)

           gtAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCACTTGTTTGACGCTCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCCGCTTCACAGGGGGG‑CCCCAAT‑AGCGGGCCGATTGGTCACACCCGGCACTCCACAAA‑CCATAAAAATGTTCGGT‑AT‑CAACCGCCTCTTCa                                                                                                                                                                                                                 >  1:403354/1‑238 (MQ=255)

             aaCTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCACCTGAGTAATTAATACGCCAGCCTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCCACA‑TGCGGGCGGATTGCCTCCGCGCGGCCTTTCACAGA‑CCCTAATAATGTTCGGT‑AT‑CAACCGCCTCTTCAc                                                                                                                                                                                                                >  1:395460/1‑237 (MQ=255)

              aCTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGGGG‑CCCAAAT‑GGCGGGCGATTTGCCTACTCGCGGCATTCCCCAGA‑CCCACATAATGTTCGGT‑AT‑CAACCGCCTCTTccacc                                                                                                                                                                                                              >  1:60369/1‑234 (MQ=255)

                   cGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCACCTGAGTAATTAATACCCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCCAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACATA‑CCCTAATAATTTTCGGG‑AT‑CAACCGCCTCTTTACCCTGTTGa                                                                                                                                                                                                        >  2:1116193/1‑239 (MQ=255)

                    gTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAGGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGAt                                                                                                                                                                                                       <  1:173550/239‑1 (MQ=255)

                          tGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTTACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTTCTCACCCCCGGCATTCCCCAGA‑CCATAAAAATTTTCGGT‑TT‑CAAACGCCTCTTCACCCTGTTGATAAGGaa                                                                                                                                                                                                 >  2:800347/1‑239 (MQ=255)

                          tGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTTCTTACGCGCGGCCTTCCCCAGA‑ACACAATAATGTTCGGT‑AT‑CAACCGCCTCTTCCCCCCGTTGGTAAGGaa                                                                                                                                                                                                 >  1:1534500/1‑239 (MQ=255)

                          tGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGaa                                                                                                                                                                                                 >  2:792809/1‑239 (MQ=255)

                          tGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCCCACCCGCGGCATTTCCCAGA‑CCAAAATAATGTTCGGG‑AT‑CAACCCCCTCTTCCCCCCGTTGATAAGGa                                                                                                                                                                                                  >  1:760155/1‑238 (MQ=255)

                          tGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTCTTTGACCCTCCGCACCCGAGTAATTAATACGCCAGACTGCCCTACCAGGAAGCGACCCGGCTCCATTTCCAGTTTCAAAGGGGGG‑CCCACAT‑GGCGGGGGATTTGCTTAACCCCCGCACTCCACAGC‑CCCTAAAAAAGCTCGGG‑AT‑CAACCCCcttcttaccccggtgctcaagaa                                                                                                                                                                                                 >  1:588533/1‑218 (MQ=255)

                            ccGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGGTTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCCCCTCCTCACCCTTTTGATAAGGAACa                                                                                                                                                                                               >  1:1288971/1‑239 (MQ=255)

                               ggCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGaa                                                                                                                                                                                            >  2:913386/1‑239 (MQ=255)

                                          tCGTTGACCCCGGCATCAACAAGCACAAAGTGGCGGCTCCCCATTGGTTTGGCGCTCCGCACCTGAGTAATTAATCCGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGC‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCAccg                                                                                                                                                                                   <  2:335734/237‑1 (MQ=255)

                                           cGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCCATTTCCAGTTTCACAGGGTGG‑CCCACAT‑GGCGGGCGATTTGCTCACGCCCGGCATTCCCCAGA‑CCATAATAATGTTCGGT‑AT‑CAACCCCCTCTTCACCCCGTTGATAAGGGACAGAAAACCCCccgcc                                                                                                                                                                                 >  1:1086405/1‑238 (MQ=255)

                                            gTTGAGCCCGGAATCAACCAGCCCAAGGGGGCGGCTCCCCATTTGTTTGCCGCTCCGCACCTGAGTAATTAATACGCCAGACTGGGCTACCAGGAAGCGACCCGGTTCAATTCCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCAccgcccg                                                                                                                                                                               <  2:911911/239‑1 (MQ=255)

                                            gTTGACCCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCAccgcccg                                                                                                                                                                               <  2:39086/239‑1 (MQ=255)

                                              tGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTTACGCTCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACCGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCCTTTGATAAGGAACAAAAAGCCCACCGCCCGCa                                                                                                                                                                             >  1:355482/1‑239 (MQ=255)

                                              tGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGAAGCTCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCCAAT‑GGCGGGCGCATTTCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CCACCGCCCCTTCCCCCTGGTGATAAGGAACAGAAAACCCACCGCCCCCa                                                                                                                                                                             >  2:160439/1‑239 (MQ=255)

                                                  cccgccaacacccgtcccaAATTGGGGGCTCCCCATGTTTGTGACGCTCCGACCCGGAGTAATTAATCCTCCAGACTGCCATCCCAGGGAGCACCCCGGTTCAAGTCCCGGTCTCCCGGGGTGG‑CCAAAAT‑GGCGGGCGATTTGCTCCCGCGCGGCATTCCACAGA‑CCATAAAAATGTGCGTT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGaaa                                                                                                                                                                         <  1:1734316/222‑1 (MQ=255)

                                                                           cggcTCCCCATTTGTTTGACGCTCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTc                                                                                                                                                >  2:252145/1‑239 (MQ=255)

                                                                           cggcTCCCCATTTGTTTGACGCTCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTc                                                                                                                                                >  2:177686/1‑239 (MQ=255)

                                                                           cggcTCCCCATTTGTTTGACGCTCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACCCGCGGCATTCCACAGA‑CCATAATAATGTTCGGG‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTc                                                                                                                                                >  2:1679644/1‑239 (MQ=255)

                                                                                          gggaccatcccgacttGAGTAATTAATACGCGCGCCTGCGCTACCGGGAAGGGCCCGTGTTAAATTCCCAGTTTCCCAGGGTGT‑CACAAAG‑GGCGGGCGTGTGGCCCCCGCGCGCCATCCCA‑AGACCCATAAT‑CGGTTCCGTCAC‑AAACCGACACGTCACCCTGTTGATAAGGCACAGAAAGCGCACCGACCGCAGAAATAGCCTGTAAATCCTGACCGACTTCGATGAAATGACgaaa                                                                                                                                 <  1:805705/224‑4 (MQ=255)

                                                                                           tgACGCTCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCCGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCAc                                                                                                                                 >  1:1630716/1‑238 (MQ=255)

                                                                                           tgACGCTCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCAcc                                                                                                                                >  2:624782/1‑239 (MQ=255)

                                                                                            gACGCTCCGCACCTGAGTAATGAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCAAAAT‑GGCGGGCGATTTGCTCACGCGGGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCa                                                                                                                               <  2:909418/239‑1 (MQ=255)

                                                                                               gCTCCGCACCTGAGTAATTAATCCGCCAGACTGCGCTCCCAGGAAGCGCCCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAg                                                                                                                            <  2:760155/239‑1 (MQ=255)

                                                                                               gCTCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTCCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGCCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAg                                                                                                                            <  2:233067/239‑1 (MQ=255)

                                                                                               ccTCCGCACTTGAGTAATTAACACGCAAGCCGGGGCCACCGGGAAGCGACCGTGTTAAATTACCAG‑TTCACCGGGGGGCCCCAAAT‑GGCGGGCGTTTTCCTCCCGCGCGGCATCCCAAAGA‑CCAAAAAAATGTTCGGT‑AC‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAg                                                                                                                            <  1:1199364/238‑1 (MQ=255)

                                                                                                   cGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGGTTTGATCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCGCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCACCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGTTGCCCTGACACACCATAGcccc                                                                                                                        >  1:19721/1‑239 (MQ=255)

                                                                                                   cGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGcacc                                                                                                                        >  2:1245008/1‑239 (MQ=255)

                                                                                                   cGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGcacc                                                                                                                        >  2:580224/1‑239 (MQ=255)

                                                                                                   cGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGcac                                                                                                                         >  2:1562112/1‑238 (MQ=255)

                                                                                                    gccccTGAGTAATTAATACGCAAGACTGCTCTACCAGGAAGCGACCCGGTTCAATTCCCATTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTGGCCCACGCGCGGCATTCCACAGA‑CCATAATAGTGTGCGGT‑AC‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGcacca                                                                                                                       <  1:230230/236‑1 (MQ=255)

                                                                                                    gcacaagAGTAATTAATCCGCCAGACTGCGCTACCAGGAGCGGCCCCGGTTCAATTTCAAGTTTCACAGGGTGG‑CCCAAAT‑GAGGGGCGATTTGCGCACCCGCGGCAGCCCACAGA‑CCATAAAAATGTCCGTT‑AC‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGcacca                                                                                                                       <  1:1416420/233‑1 (MQ=255)

                                                                                                    gCACCTGAGTAATAAATACGCCAGACTGCGCTCCCAGAAAGCGACCCGGTTCAATTTCCAGCTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGC‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGcacca                                                                                                                       <  2:403354/239‑1 (MQ=255)

                                                                                                    gCACCGGAGAAATTATTCCGCCAGCCGGCGCTACCAGGAAGCCACCCGGTCCAATTTCCAGTTTCCCAGGGTGG‑CCAAAAG‑GGCGGGCGATTTGCTCCCCCGCGGCATTCCACAAA‑CCATAATAATGTTCGTT‑AC‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGcacca                                                                                                                       <  1:223294/239‑1 (MQ=255)

                                                                                                       ccTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACcacac                                                                                                                    <  2:1086405/239‑1 (MQ=255)

                                                                                                         tGATTAATTAATCCGCCGACCTGCGCTCCCGGGAAGCGACCCGTTTAAATTTCCAGTTTCACAGGGGGG‑CCAAAAT‑GGCGGGCTATTTGCTCCCGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCacacac                                                                                                                  <  1:1292301/239‑1 (MQ=255)

                                                                                                           aGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACcacaca                                                                                                                   >  2:230230/1‑236 (MQ=255)

                                                                                                           aGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCt                                                                                                                >  1:980185/1‑239 (MQ=255)

                                                                                                             taataagtccGCCAGACTGCGTCACCGGGAACCGACCGGTGTCAATTTCCTGTTTCACGGGGGGC‑CCCAAAG‑GCCGGGCGTTTTCCCCCCGCGCGGCATTCCACAGA‑CCAAAAAAATGTTCGGT‑AC‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGcacca                                                                                                                       <  1:460753/221‑1 (MQ=255)

                                                                                                                 taatCCGCCAGACGGCGCTACCAGGAAGCGACCCGTTTCAATTCCCAGTTTCACAGGGTGG‑CCAAAAG‑GGCGGGCGATTTGCCCACGCGCGGCATCCCACAGA‑CAAAAATAAGGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACAcc                                                                                                                 <  1:44485/232‑1 (MQ=255)

                                                                                                                   ctacgacacacgccgcacccgggAACCGCCCCTGTTTATTTTCCAGTTTCGCAGGTTGC‑CCAAAAT‑GGGGGGCTTTTGGCTCACGCCGGGCATTCAACAGA‑CCAAAAAAATGTCGGGT‑AC‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGa                                                                                                        <  1:624782/218‑1 (MQ=255)

                                                                                                                     cccccacacgccgcTCCCAGGAAGGACCCCGGTTCAATTTCCAGTTCCCCAGGGTGC‑CCCAAAT‑GGCGGGCGATTTGCTCCCGCGCGGCATTCCACAGA‑CCATAAGAAGTTGCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGa                                                                                                        <  1:160439/226‑1 (MQ=255)

                                                                                                                       gCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGtt                                                                                                             >  2:460753/1‑230 (MQ=255)

                                                                                                                         cAGACTGCGCTCCCAGGAGGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCCCGCGCGGCATTCCACAGA‑CCATAAAAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCa                                                                                                  <  2:395460/239‑1 (MQ=255)

                                                                                                                               ggcgtACCAGGGAGCGACCCGGTGAAATTCCGTGGTACCGAGGGGGG‑ACAAAGT‑GGTGGGCGTTTGCCTCCCCCGCGGATTTCCCAGGC‑CCATAATAAGGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCGGCCGCACCATAGCACCGCACACCTGTTCCAGATGGGCATAATCa                                                                                            <  1:1017825/235‑1 (MQ=255)

                                                                                                                                 gcTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCaa                                                                                           >  2:349290/1‑238 (MQ=255)

                                                                                                                                   tACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACg                                                                                         >  2:1255362/1‑238 (MQ=255)

                                                                                                                                          aaGCGACCCGGTTCAATTTCCATTTCCACGGGGTGG‑CCAAAAT‑GGCGGGCGATTTCCTCACGCGCGGCATTCCACAGA‑CCAAAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAAc                                                                                  <  1:792809/238‑1 (MQ=255)

                                                                                                                                           aGCGACCGGGTCCAATTTCCAGTTCACCAGGGTGC‑CCCAAAT‑GCCGGGCAATTTGCCCACGCGCGGCATCCCACCGA‑CAATAATAAGTTCCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAAACCTGACCGAAGTCGATGACCTGACGCACCATAGCACCACACCCCTGTTCCAGAGGGGCATAATCAACGCCAGAACCa                                                                                <  1:1592732/239‑1 (MQ=255)

                                                                                                                                             cgccccGTTCCAATTTCCAGTTTCCCAGGGTGG‑CCCAAAG‑GGCGGGCGATTTGCCCCCGCGCGACATCCCACAGA‑CCAAAATAATGTTCGTT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCa                                                                                <  2:588533/234‑1 (MQ=255)

                                                                                                                                              gACCGGGTTCAATTCCCAGTTTCACGGGGTGG‑CCAAAAT‑GGCGGGCGATTTGCCCACGCGCGGCATTCCAAAGA‑CCAAAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAAt                                                                              <  1:1562112/238‑1 (MQ=255)

                                                                                                                                               aCCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAAtgt                                                                            >  1:1016997/1‑239 (MQ=255)

                                                                                                                                               aCCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAAtgt                                                                            >  2:1436860/1‑239 (MQ=255)

                                                                                                                                               aCCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAAtgt                                                                            >  1:375982/1‑239 (MQ=255)

                                                                                                                                               aCCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAAtgt                                                                            >  1:603663/1‑239 (MQ=255)

                                                                                                                                                   ggTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCAt                                                                        >  2:1592732/1‑239 (MQ=255)

                                                                                                                                                          tttCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGCTTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAAt                                                                  >  1:1387860/1‑238 (MQ=255)

                                                                                                                                                                     acaGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTg                                                      <  2:375982/239‑1 (MQ=255)

                                                                                                                                                                        gggTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAg                                                   >  1:1664852/1‑239 (MQ=255)

                                                                                                                                                                          gTGG‑CCCAAGT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CAATAATAATGTTCGGT‑AT‑CACCCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAga                                                  <  1:572675/238‑1 (MQ=255)

                                                                                                                                                                           tGG‑GCCAAATGGGCGGGCGTTTCGCTCCCGCGCGGCATCCCACAAC‑CAAAAAAAATTTTCGGA‑AC‑CAACCGCCTCTTCACCCTTTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAga                                                  <  1:1086141/238‑1 (MQ=255)

                                                                                                                                                                               cccAAAT‑GGCGGGCGTTTTGCCCCCGCGCGGCATTCCACAGA‑CCAAAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAgatgat                                              <  1:177686/238‑1 (MQ=255)

                                                                                                                                                                               ccaaaaG‑GGCGGGCGATTTGCTCACGCGCGGCATTCCAAAGA‑CCATAAAAATGTTCGTT‑AC‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAgatgat                                              <  1:1668374/235‑1 (MQ=255)

                                                                                                                                                                                ccAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAGCCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGatgatg                                             <  2:1373022/238‑1 (MQ=255)

                                                                                                                                                                                  aaaT‑GGCGGTCGGTTTGCCCAGGGCCGGAATTCACCAGC‑CCAAAATAA‑GGGCGGT‑TTACAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCAACACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACg                                          <  1:265339/239‑1 (MQ=255)

                                                                                                                                                                                  aaaT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGTT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACg                                          <  1:580224/239‑1 (MQ=255)

                                                                                                                                                                                    aT‑GGCGGGCGATTTGCTCACCCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGCAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGtt                                        >  1:70622/1‑239 (MQ=255)

                                                                                                                                                                                         cgggcgATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTa                                     >  2:1020397/1‑238 (MQ=255)

                                                                                                                                                                                         cgggcgATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAACACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTa                                     >  2:757054/1‑238 (MQ=255)

                                                                                                                                                                                           ggcgATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACg                                          >  1:1464285/1‑231 (MQ=255)

                                                                                                                                                                                           ggcgATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACg                                          <  2:1464285/231‑1 (MQ=255)

                                                                                                                                                                                           ggcgATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATc                                   <  2:355482/238‑1 (MQ=255)

                                                                                                                                                                                                      cACGCGCGGCATCCCACAGA‑CAAAAAAAATGTTCGGT‑AC‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTgcg                       <  1:913386/239‑1 (MQ=255)

                                                                                                                                                                                                                atcccACACA‑CAAAAATAAATTCCGGT‑AT‑AACCCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGAt             <  1:81544/236‑1 (MQ=255)

                                                                                                                                                                                                                aTTCCCCAGA‑CCATAATAAGGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGAt             <  2:60369/239‑1 (MQ=255)

                                                                                                                                                                                                                aTTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGAt             <  2:1016997/239‑1 (MQ=255)

                                                                                                                                                                                                                aTTCCACAGA‑CAATAAAAATCTGCGGC‑AC‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGAt             <  1:800347/239‑1 (MQ=255)

                                                                                                                                                                                                                  cccACAGA‑CAAAAAAAATGTTCGGT‑AT‑AAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCg           <  1:1679644/238‑1 (MQ=255)

                                                                                                                                                                                                                   ccACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCgg          >  2:716273/1‑239 (MQ=255)

                                                                                                                                                                                                                    cacaGC‑CCATAAAAATGTTCGGT‑AC‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGgt         <  1:1255362/239‑1 (MQ=255)

                                                                                                                                                                                                                    cacaGA‑CCATAATAATGTTCGTT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGgt         <  2:1624230/239‑1 (MQ=255)

                                                                                                                                                                                                                    cacaGA‑CAATAAAAACTGTCGGA‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCCCGTCCAGTGCGGCGGGCAGATCGgt         <  1:155881/239‑1 (MQ=255)

                                                                                                                                                                                                                     cacggcccAAAATAATGTCCGGT‑CT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGgt         <  2:19721/233‑1 (MQ=255)

                                                                                                                                                                                                                         a‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCCGTGCGGCGGGCAGATCGGTGTAc     >  1:59172/1‑238 (MQ=255)

                                                                                                                                                                                                                         a‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGGGGTAcc    >  2:1207665/1‑239 (MQ=255)

                                                                                                                                                                                                                         a‑CCACAATAACGTTCGGT‑AC‑AAGCCGCCGCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGca                                                                                                                          <  1:994311/121‑1 (MQ=255)

                                                                                                                                                                                                                           ccATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGGTGTAcc    <  2:969567/238‑1 (MQ=255)

                                                                                                                                                                                                                            cATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGCTCGGGGTAcccg  >  1:142790/1‑237 (MQ=255)

CTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGG‑CCCAAAT‑GGCGGGCGATTTGCTCACGCGCGGCATTCCACAGA‑CCATAATAATGTTCGGT‑AT‑CAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGGTGTACCAG  >  NC_000913/2977945‑2978400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`