BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,206,469	C→G	15.9%	S3R (AGC→AGG) ‡	ttdA →	L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,469	0	C	G	15.9%	187.5 / 12.6	106	S3R (AGC→AGG) ‡	ttdA	L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand): ref base C (32/47); new base G (11/5); total (47/59)
Fisher's exact test for biased strand distribution p-value = 5.37e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 109 total aligned reads displayed.

TAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGACAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAG  >  NC_000913/3206234‑3206707

tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGACAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑Ga                                                                                                                                                                                                                                                  <  2:1651855/238‑1 (MQ=255)

       caacTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCAATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGACAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGtaat                                                                                                                                                                                                                                           <  1:1662194/235‑1 (MQ=255)

       cAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGACAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGtaat                                                                                                                                                                                                                                           <  2:1373669/238‑1 (MQ=255)

       cAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGACAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGtaat                                                                                                                                                                                                                                           <  2:291732/238‑1 (MQ=255)

         gCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑GG‑AATTTTTTCCGACAAATAA‑AAAAAATGGA‑GTTGTCATGTTGGGT‑GAAAGAAAAAAg                                                                                                                                                                                                                                        >  2:1519060/1‑239 (MQ=255)

                     cGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑GATTTTTTCAGACAAATAC‑AAAAAACGGG‑GTTGGCCTGATGTGC‑GAAAGAAATAAAAAAA‑AGGCAggg                                                                                                                                                                                                                           >  2:550144/1‑237 (MQ=255)

                     cGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAAACAAATAC‑AAAAAATGGA‑GATGTCATGATTAGA‑GAAAAGAAAAAACAAC‑AAGCAATg                                                                                                                                                                                                                           >  2:1580551/1‑239 (MQ=255)

                       ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTTACAACAAAAC‑AAAAAATGAA‑GTTTTCATGAGGAGC‑GAAAGGAATAAACAAA‑AGGCAGTGaa                                                                                                                                                                                                                         >  2:792084/1‑239 (MQ=255)

                       ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AAATTTTTCTGACAAAATA‑AAAAACAGGA‑GTTTGCATGGTTGGC‑GGAAGgaataaagaaaagggaaggaa                                                                                                                                                                                                                          >  1:417638/1‑224 (MQ=255)

                        tAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGACAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGaa                                                                                                                                                                                                                         <  1:158652/238‑1 (MQ=255)

                           ttATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGACAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAg                                                                                                                                                                                                                     <  1:542581/239‑1 (MQ=255)

                                cGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGGTTG‑AATTTTT‑CTGACAAAAAA‑AAAAAATCGG‑GGTGCCCTTTTGTGG‑GATAGTAATAAACAAC‑ACGCAGTGAaaaaagtta                                                                                                                                                                                                                 >  2:778295/1‑233 (MQ=255)

                                      tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGACAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAgag‑a                                                                                                                                                                                                          <  1:1603904/238‑1 (MQ=255)

                                             aGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AAATTTTTCAGAAAAATAA‑AAAAAATGGA‑GGTGGCATGATGAGC‑GAAAATAATAAAAAAC‑AAGACGTGAAAAAGATGACAGAG‑AATGACGC‑t                                                                                                                                                                                                 >  2:1556180/1‑239 (MQ=255)

                                               cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑TATTTTTTCAGACAAATAC‑AAAAACAGGG‑GTTTGCACGATGAGC‑GAAAGTAATTAGCAAA‑AAACACAGAATAAATTTGCAGAG‑GTTGTCGC‑Taa                                                                                                                                                                                               >  2:1273840/1‑239 (MQ=255)

                                               cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTTAGACAAATAA‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGAGAATAAATTGACAGAG‑ATTGTCGC‑Taa                                                                                                                                                                                               >  2:1152676/1‑239 (MQ=255)

                                               cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑AG‑AAATTTTTCAGGCCAATAC‑AAAAAATGGA‑GGTTCCATTATGGGG‑GGAAGAAATAAACAAC‑AGGCAGTGACAAAGATGACAGAA‑ATTGtgtccaa                                                                                                                                                                                                >  2:1185310/1‑233 (MQ=255)

                                                  agtttttttCGCAAAACATTGCCATCCCCTGTTCTGGCGAAATATTTATTCTTATTTTCGGTTTGATTCACGAACTGTTAATAACATATTCGGGGTGAAAAAAAATAAAAAACCAC‑ATTTTTA‑GTGGTAGTTTGGGGGGG‑TA‑AATTTTTCCAGACAAATAC‑AAAAACGGGA‑GTTGCCATGATGAGC‑AAAATAAATAAGCAAC‑AGGCAGTAAATAAGTTGACAGAG‑ATTGTCGC‑TAACtt                                                                                                                                                                                            <  1:550144/233‑1 (MQ=255)

                                                         ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCCGACAAAAAC‑AAAAACTGGA‑GGTGGCATGATTAGC‑GGAAGAAATAAACAAC‑AAGCAGTGAATAAAGTGACAGAG‑ATTGTTGC‑TAACTTTACCGc                                                                                                                                                                                      >  2:1069861/1‑238 (MQ=255)

                                                              aaaaCATTGCCATTCCCTGCTCTGGCGAAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGAAAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGAAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑At                                                                                                                                                                               <  2:1430263/239‑1 (MQ=255)

                                                              aaaaCATGGCCATTCCCTTCCCTGGCGAAATTTTCATTCTTATTTCCACGTTGATTCACGAATTGTTAATTACATATCCAGGGTGGCAAAAAATATAAACCCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGACAAAAAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑GGGAAGTGAATAAGTTGACAGAG‑ATTGTCGT‑TAACTTTACCGCCATG‑At                                                                                                                                                                               <  2:573091/239‑1 (MQ=255)

                                                                aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTTCGTCAAAAAC‑AAAAAATGTA‑GTTGTCATGGTGAGC‑GAAAGTAATAAGAAAA‑AGGCAGTGAAAAAAATGGTAGAA‑ATTTTCTC‑CAACTTTTCCCCCCTG‑Agtt                                                                                                                                                                             >  1:850490/1‑239 (MQ=255)

                                                                aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCCCAAAAAAAA‑AAAAAATGGA‑GTTGCCATTATGAGC‑GGAAGTAATAAACAAA‑AAGCAGTGAATAAGATGACAGAG‑ATTGTCGC‑TAACTTTTCCGCCCTG‑Attt                                                                                                                                                                             >  2:860709/1‑239 (MQ=255)

                                                                aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAGACAAATAA‑AAAAAATGGG‑GTTTGCCTGGTGAGG‑GAAAGTAAAAAAAAAA‑CAGCAGTGAAAAAAGTGAAAGAG‑AGTGTGTC‑TAACttttaccccataaatt                                                                                                                                                                              >  1:881903/1‑226 (MQ=255)

                                                                     tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTTCGACAAAAAC‑AAAAACATGA‑GTTGCCATGATGAGA‑GGAAAGAATAAGCAAA‑AGGCAATGAATAAGATGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCttc                                                                                                                                                                         >  2:652316/1‑236 (MQ=255)

                                                                     tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCCGACAAAAAC‑AAAAAAATGG‑GTTGGCCTTGTTAGG‑GAAAGGAATAAAAAAA‑AGGCAGTGAATAAATTGACAGAG‑ATTGTTGC‑TAACTTTACCCCCCTG‑ATTTTTAc                                                                                                                                                                         >  2:1082752/1‑238 (MQ=255)

                                                                        cATTCCCTGCTCTGACTAAATATTCTTTCTTTTTTTCGAGTTGATTCACGAACTTTTAATTACATTTTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCACAAAAATAC‑AAAAACGGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAATTTAACAGAG‑ATTGTCTT‑TAACTTTACCGCCATG‑ATTTCTACCCGa                                                                                                                                                                     <  1:1350825/239‑1 (MQ=255)

                                                                         aTTCCCTGCTCTGGCGAAATATTCATTCTTATTTTCGCGTTGATTCCCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGTTAGTTTGTGGCGT‑TA‑AATTTTTCCAGACAAATAC‑AAAAACTGGA‑GTTGCCATGAAGAGC‑GAAAGTAATAAGCAAC‑GGGCAGTGAATAAGTTAACAGAG‑ATTGTCCC‑TAACTTTACCGCCATG‑ATTTCTACCCGaa                                                                                                                                                                    <  2:1501440/239‑1 (MQ=255)

                                                                            cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAAACAAAAAA‑CAAAAATGGA‑GTTTCCCTGAGTAGC‑GAAAAAAATAAACAAC‑AGAAAGTGAATAAGTTGGAAGAG‑ATAGTCGA‑GAACTTTTCAGCCCAG‑CTTTATATCCGaaag                                                                                                                                                                  >  1:838519/1‑236 (MQ=255)

                                                                            cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGACAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑t                                                                                                                                                                                                 <  2:529707/208‑1 (MQ=255)

                                                                               tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AACTTTTTTCGACACATAC‑AAAAACTCGA‑GTTGCCATGGTGAGC‑GGAAGTAAAAAAAAAC‑ACGAACTGAAAAATATGAAAGAG‑ATTGTTGA‑TAAATTTACCGCCAttagtttcatccctaagcctt                                                                                                                                                               >  1:800609/1‑219 (MQ=255)

                                                                                 ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAGACAAAAAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AAGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAAATTTTTCCCCCTG‑ATTTTTACCCCAATGCCtgat                                                                                                                                                            >  2:292034/1‑239 (MQ=255)

                                                                                 ctctGGCGAAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAACCCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGAAAAATAA‑AAAAACGGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCa                                                                                                                                                                                    <  2:577569/216‑1 (MQ=255)

                                                                                  tcgggCGCAATATTCATTCTTATTTTCGAGTGAATTCACGAACTGTTAATTACATATCCAGGGTGGCAAAAAAAATAAACCCAC‑ATTTTTA‑GTGGTAGTTTGGGGGGG‑TA‑AATTTTTCCAGACAAATAC‑AAAAACGGGA‑GTTGCCTGGAGGGGG‑GAAAGTAATAAGCAAC‑AGGCAGTGAAAAAGTTGACGGAG‑ATTGTCGT‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCtgatg                                                                                                                                                           <  1:584548/236‑1 (MQ=255)

                                                                                    tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAACCCAC‑ATTTTTA‑GTGGTATTTTGGGGCGG‑TG‑AATTTTTCCAAACAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑AAAATTAATAAGCAAC‑AGGAAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGAc                                                                                                                                                         <  2:422094/239‑1 (MQ=255)

                                                                                     ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCTAACAAATAC‑AAAAAATGGA‑GTTGTCATGATTAGT‑GAGAGTAAAAAAAAAA‑CGACAATGAATAAATTGAAAGAG‑AATGTCGC‑TAACTTTAACGCCCTG‑ATTTTTATCCGAATGCCtgaggagg                                                                                                                                                        >  1:1584633/1‑237 (MQ=255)

                                                                                      gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTTATAAAAAAAA‑AAAAAATGGA‑GGTGGCCTGGTGAGC‑GGAAGTAATAAACAAC‑AGGGAAGGAATAAATTGACAAGG‑ATTGTCGC‑TAACTTTTCCCCCCTG‑ATTTCTTCCCCAATGTCttgtggcgt                                                                                                                                                       >  1:164378/1‑231 (MQ=255)

                                                                                      gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAGACAAAAAC‑AAAAAATGGAGGTTGCC‑TGATGAGC‑GAAAGTAATAAGCAAC‑AAGCAGAGAATAAGTTGACAGAG‑GTTGTCGC‑CAAATTTACCGCCCTG‑ATTTCTACCCGAATGCCTGATGACgt                                                                                                                                                       >  1:308395/1‑239 (MQ=255)

                                                                                       cgcAATATTTATTTTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATTCACGGGGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGGGG‑TG‑AATTTTTCCAGACAAATAC‑AAAAACGGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAT‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACgt                                                                                                                                                       <  1:1612242/238‑1 (MQ=255)

                                                                                           atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑GATTTTTTCAAACAAATAC‑AAAAAATGGA‑GTTTCCCTGATGAGG‑GAAAGTAATAAACAAC‑AAGCAGAGAATAAATTGACAGAG‑ATTGTCGC‑TAAATTTACAGCCCTG‑ATTTCTaa                                                                                                                                                                         >  1:577569/1‑215 (MQ=255)

                                                                                                    cTTATTTTCGTTTTGATTCACGACCTGTTAATTACATATTCACGGTGGAAAAAAAAATAAAACCAC‑ATTTTTA‑GTGGTGGTTTGGGGCGG‑TG‑AATTTTTCAAGACAAATAA‑AAAAACTGGA‑GTTCCCATGATGAGC‑GAAAGTAATAAGCACC‑AGGCAGTAAATAATTTGCCAGAG‑ATTGTCTC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa                                                                                                                                         <  2:621385/239‑1 (MQ=255)

                                                                                                      tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTTTGACAAAAAC‑AAAAAAAGGA‑GTTGCCATGATGAGC‑GAAAGTAAAAAACAAC‑ACGCCGTGGATAAGGTTACCGAG‑ATTTTCGC‑TAACTTTACCCCCACG‑CTTTTTACCCCAAAGTCCGCTTGCGTGGTGGGTATACTaaaa                                                                                                                                       >  1:1313315/1‑239 (MQ=255)

                                                                                                       aaagatgtgtaaaagagaaaggtGTTAATTACATATTCACGGTGGAAAAAAATAAAAAACCAC‑ATTTTTA‑GTGGTAGTTGGTGGCGG‑TG‑AATTTTTCCAGAAAAATAC‑AAAAACGGGA‑TTTGCCATGATGAGC‑GAAAGAAATAAGAAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                       <  1:985178/216‑1 (MQ=255)

                                                                                                            cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGACAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑AAAAGTAAAAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTa                                                                                                                                 <  2:448102/239‑1 (MQ=255)

                                                                                                                 ggATTCACGAACTTTAAATTACATATTCAGGGTGGAAAAAAATAAAAAACCAC‑ATTTTGA‑GTGGTAGTTGGTGGCGG‑TG‑AATTTTTCCAGACAAATAC‑AAAAACGGGA‑TTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTGGACAGAG‑ATTGTCGC‑TAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGa                                                                                                                            <  1:1302414/238‑1 (MQ=255)

                                                                                                                       aCGAATTTTAAATTACATTATCACGGTGCCAAAAAAAAGAAACCCAC‑ATTTTTA‑GTGGGAGTTTGTGGCGG‑AG‑AATTTTTCCAGAAAAATTG‑AAAAACGGTA‑GTTGCCTTGAGGAGC‑GAAAGTAA‑AAGCCACTGGGCAGTAAATAAGTTGATAGTG‑TTTGTCGC‑TACCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGa                                                                                                                      <  2:1224552/239‑1 (MQ=255)

                                                                                                                             tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TGAAATTTTTCCA‑ACAAAAAA‑AAAAAATGGA‑GGTGGCATGGTTGAC‑GAAAATAAAAAACAAA‑AAGCAGGGGAAAAATTGATAGAG‑ATTGTCGCTTACCTTTAAC‑CCATA‑ATTTTTTCCCGAACAGCTGATGACGGGGGGGGTTAAATAAAAAAAATAAAGGAAAGCGAAAcca                                                                                                                 >  1:1021539/1‑236 (MQ=255)

                                                                                                                             tGTTAATTAAATATTCACGGTGGCAAAAAATAAAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGACAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                       <  2:389060/216‑1 (MQ=255)

                                                                                                                             tGTTAATGACATATTCACGGTGGAAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGACAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGGAGGGAATAAGTTGACAGAG‑ATGGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACgtgg                                                                                                                                                     <  2:371281/202‑1 (MQ=255)

                                                                                                                               ttAATTACATATTCGCGGTGGAAAAAAATAAAAACCCAA‑ATTTTAA‑TGGGTATTTTGGGGGGG‑GA‑AATTTTTCAAAAAAAAAAC‑AAAAACGGGA‑GTTGCAATGATGAGC‑AAAAGAAATAAGCAAC‑AGGCAGTGAAAAAGTTGAAAGAG‑TTGGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAcgtcgt                                                                                                              <  1:1082752/239‑1 (MQ=255)

                                                                                                                                 aaTTATAAATTAGCGGTGG‑AAAAAAAAAAAAACCACAATTGTAA‑GGGGTATTTTGTGGCGG‑TA‑AATTTGTCCAGAAAAATAA‑AAAAGTAGAA‑GTGGCCAGTATGAGA‑GAAAGAAAAAAACAAC‑AGCCTGTAAAAACGTTAACAGCG‑ATCGTCTC‑AACCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGtcgtcg                                                                                                            <  1:1273840/239‑1 (MQ=255)

                                                                                                                                       atatTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCCGACAAAAAC‑AAAAACTGGA‑GTTTCCCTTTTGTGC‑GGAAGTAATAAACCAC‑CGGCAGTGAAAAAATTGGCAGAG‑ATTGTTGC‑TAAATTTACCGCCCTG‑ATTTTTTCCCGAAATCCTTGTTACGCGGTGGATAAAATAAAAAAACTAAAAGactgtctcttataaccctctccc                                                                                                      >  2:985178/1‑217 (MQ=255)

                                                                                                                                       atatTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAGACAAAAAA‑AAAAAATGGG‑GTTGGCATGATGAGC‑GAAAATAATAAAGAAC‑AAGCAGGGAATAAATTGACAGAGAATTGT‑GC‑CAAATTTACCGCCCTG‑ATTTTTAACCGGATGCCTGATGACGGGGGGGGTAAACTAAAAAAGCTAAAAGAAGCCGAAACCTCGCCGATgggg                                                                                                      >  2:1707341/1‑239 (MQ=255)

                                                                                                                                        ttttagaggtgcaaaaaaaGAAAAACCCAC‑ATTTTTA‑GTTGTTGTTGGGGGCGG‑TA‑AATTTTCCCAGAAAAATAC‑AAAAAATGAA‑TTTGCATTGATGAGA‑GAAATTAATAAGCAAC‑AGGCGGTGAATAAGTTGACAGAG‑ATTGTCGT‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                     <  2:1406998/226‑1 (MQ=255)

                                                                                                                                        tatgcgcGGTGGCAAAAAATAAAAAACCAA‑ATTGTTA‑GTGGTAGGTTGGGGGGG‑TG‑AATTTTCCAAGACAAAAAC‑AAAAACTGGG‑GTGGCAATGAGGAGC‑AAAAAAAATAAGCAAC‑AGGCAGTGAATAATTGAACAGAG‑ATTGTGGT‑AAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                     <  1:77868/233‑1 (MQ=255)

                                                                                                                                        tatTCACGGTGGAAAAAAATAAAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAAAAAAATAA‑AAAAAGTGGA‑GTGGCCATGATGAGC‑AAAATGAAAAAGCAAC‑AGGCAGTGAATAAGTTGAAAGGT‑ATTGGCGC‑TAATTTAACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                     <  2:1589960/239‑1 (MQ=255)

                                                                                                                                        atgtctgtggggaaaaaaaaaaaaaACCCC‑GTTTGTG‑GTGGTAGGTTGGGGCGG‑TG‑AATTTTTCCAGACAAATAA‑AAAAACTGGA‑GTGCCCAGCAAGGGA‑AAAAGAAAGAAGCAAC‑AGGCAGTGAAAAAGTTGAGAGAG‑ATTGCCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                     <  1:499646/226‑1 (MQ=255)

                                                                                                                                         atTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCTGACAAAAACAAAAAACTGG‑‑GTTGGCCTGATGTGC‑GAAAGTAAAAAACAAC‑AAGCAAGGAATAAATTGACAGAG‑ATTGTCGC‑TAAATTTAACGCCATG‑AATTTTTCCCGAATGCCTGTTGACGTGGTGGGTAAAATAAAACAGCTAAAGGATGCCCGAACGTCGTCGATggggga                                                                                                    >  2:1625485/1‑237 (MQ=255)

                                                                                                                                         atTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAGAAAAAAAA‑AAAAAATGGA‑GGTGTCATGTTGAGC‑GAAAAGAATAAAAAAA‑AGGCAAGGAAAAAGTTGACAGAG‑AGTTTTGC‑TAAATTTTACGCCCTG‑ATTTCTACCCGAATGCCTGTTAACGTGGGGGATAAAATAAAAAAGCAAAAAGAAAACCAAAACACGTCGATGGGGaa                                                                                                    >  1:1027860/1‑239 (MQ=255)

                                                                                                                                         atTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑CG‑AAATTTTTCAAACAAAAAC‑AAAAAAAGGA‑GTTGCCACGGTGGGG‑GGAAAGAAAAAACAAA‑AAGCAAAGAAAAAATTGACAGAG‑AATGTCGC‑TAAATTTTCCCCCCTGAATTTTTAACC‑AAAGCCTGGTGACGTGGGGGGTAAACTTAAAAAAATCAAAGATACCGCAAagtcgtgcattgggga                                                                                                    >  1:1236633/1‑229 (MQ=255)

                                                                                                                                           tCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCCGACAAATAC‑AAAAAATTGA‑GTTTCCCTGATGAGC‑GGAAAAAATAAACAAC‑AAGCAGTGAATAAGTTGACAGAG‑ATTGTTGC‑TCACCTTACCGCCATG‑ATTTTTTCCCCAATGCCTGATGAAGTGGGGGGTAAACTAAAACAGCTAAAAGAAGCCCAAACATCGTCGATGGGGgaaa                                                                                                  >  2:1420966/1‑239 (MQ=255)

                                                                                                                                             aCGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAGACAAAAAA‑AAAAAAATGA‑GTTGCCCTGATGAGC‑GAAAGTAATAAACAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTTCCCGAATGCCTGATGACGTGGTGGATAAACTAAAAAAACTAAAgg                                                                                                                             >  2:965403/1‑210 (MQ=255)

                                                                                                                                                ggggCAAAAAAGAAAAAACCAT‑ATGTTTG‑GGGGTAGTTTGTGGGGG‑TG‑ATTTTTGCAAGAAAAATAC‑AAAAACTGGT‑GTTGCAATGATGAGC‑AAAAGTAATAAGCAAC‑GTGGAGTTAATAAGTGGACAGAG‑ATTGCCGT‑TACCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGAAAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                              <  1:1063215/236‑1 (MQ=255)

                                                                                                                                                gTGGAAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGACAAATAC‑AAAAACGGGA‑GTGGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                              <  2:838519/238‑1 (MQ=255)

                                                                                                                                                gTGGAAAAAAAAATAAAACCA‑‑ATTTTTAGGTGGTAGTTGGGGGCGG‑TA‑AATTTTTCCAGACAAATAC‑AAAAATTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAACAAC‑AGGCATTAAATAATTTAAAAGAT‑TTTGTCGC‑TATCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                              <  1:458088/238‑1 (MQ=255)

                                                                                                                                                 tgtaaaaaaaTAAAAACCTAA‑ATTTTTA‑GTGGTGTTTTGTGGGGG‑AA‑AATTTTCCAAAAAAAATAC‑AAAAACGAGA‑GTGCCTATGA‑GAGCAAAAAGTAATAAGCAAA‑AGACAGAGAATAAGTTGACGGAG‑ATTGTCGC‑AAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACGAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATc                                                                                             <  1:1313660/234‑1 (MQ=255)

                                                                                                                                                    aaaaaaaTATAAAACCAC‑ATTTTTA‑GTGGTGGTTTGTGGGGG‑TG‑AATTTTTCCAGAAAAATAC‑AAAAACGGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGT‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTAc                                                                                          <  2:693594/237‑1 (MQ=255)

                                                                                                                                                      aaaaaTATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAGACAAAAAA‑AAAAAATGGA‑GTTGCCATGATGAGG‑GAAAGTAATAAACAAC‑AGACAGTGAATAAGATGACAGAG‑ATTGTCGC‑TAAATTTTTCGCCCCG‑ATTTCTACCCGAATGCCTGCTGAGGTGGGGGGTAAACTAAAAAAGCTAAAGGATGCCGAAACGTCGTCGCTGGGGGAAATTATCTAccct                                                                                       >  2:78551/1‑237 (MQ=255)

                                                                                                                                                         aaaataAAACCTC‑ATTTTAC‑TTGTAAGTTTGGGGCGG‑TG‑ATTTTTTCAAAAAAAAAAA‑AAAACCTGGA‑GTTGACTTAAGTGGA‑GAACATAATAAACACC‑AGGAAGTGAATAAGTTGAAAGAA‑TTTGTCCT‑TAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                    <  2:519224/236‑1 (MQ=255)

                                                                                                                                                                aaCCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTTAAACAAAAAA‑AAAAAATGGA‑GTTTGCATTATGGGG‑GGAAGAAATAAAAAAC‑AAACAGAGAAAAAATTTACAAAG‑ATTGTCGC‑TAAACTTACCCCCACG‑ATTTTTTCCCCAAAGCCCCGTGACGGGGGGGGTAAAACAAAAAAACCAAAAGATGTCCAAACATCGCGGGTGGGGGAAAATATCTAACaacagcagttcg                                                                             >  1:139719/1‑228 (MQ=255)

                                                                                                                                                                 cccAC‑ATTTTTA‑GTGGTATTTTGTGGCGG‑TG‑AATTTTTCCAGACAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGa                                                                            <  2:1609537/238‑1 (MQ=255)

                                                                                                                                                                  ccAA‑ATTTGTA‑GTGGTTGTTTGTGGCGG‑TG‑AATTTTTACAGACAAAAAA‑AAAAACGGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGac                                                                           <  1:1185310/239‑1 (MQ=255)

                                                                                                                                                                    ac‑aTATTGA‑GTGGTAGGTGAGGGGGG‑TG‑AATTTTTAAAGAAAAAAAA‑AAAAACGGGT‑GTTCCCATGATGAGC‑GAAATAAATAAGAAAG‑AGCCAGTGAAAAAGTTGAAAGAG‑ATTGTGGG‑AAACTTTCCCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGacaa                                                                         <  1:792084/239‑1 (MQ=255)

                                                                                                                                                                     c‑aTTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AAATTTTTCAAACAAAAAA‑AAAAAATGGA‑GTTGGCATGATGAGC‑GAAAGTAAAAAACAAA‑AAGCAGGGAATAAATTGACAGAG‑ATTGTCGC‑TAACTTTAAAGCCCTA‑ATTTCTACCCGAATGCCTGATGACGGGGGGGGTAAACTAAAAAAGCTAAAGGATGCCGAAAAGTCGTCGATGGGGAGAAATATCTACCCTAAGATGTTCGacaaa                                                                        >  1:1609537/1‑238 (MQ=255)

                                                                                                                                                                           ttA‑GTGGTAGTTTGGGGCGG‑TA‑AATTTTTCCAGACAAATAA‑AAAAACTGGA‑GTTGCCATGATGAGC‑AAAAGTAAAAAGCAAC‑GGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCa                                                                   <  2:850490/239‑1 (MQ=255)

                                                                                                                                                                                  gTAGTTTGTGGCGG‑TG‑AATTTTTTCCGACAAATAC‑AAAAACTTGA‑GTTGGCCTGATGAGC‑GGAAGTAATAAACAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTTCCGCCCTG‑ATTTTTACCCCAAAGCCTGATGACGTGGGGGGTAAACCAAAACAGCTAAAAGATGCCGCAACCTCGTCGATGGGGGAAAATATATACCATACGATTTTTGACAAAATGCaaaaaac                                                             >  2:732356/1‑237 (MQ=255)

                                                                                                                                                                                    attttagggCTG‑TA‑AATTTTAACAGAAAAATAC‑AAAATCGTGA‑GTTGCAGTGATGAGG‑AAAGGTAGTAATCAAC‑AGCAAGTGAATAAGTTGCCAGAT‑TTTGTAGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGGGGTGGATAAACTAAAACAGCTAAAGGATGCCTAACCGGCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                           <  2:1229731/232‑1 (MQ=255)

                                                                                                                                                                                    aGTTTGTGGCGG‑TG‑AATTTTTCCAGACAAATAC‑AAAACCTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                           <  2:396519/239‑1 (MQ=255)

                                                                                                                                                                                    aGTTTGTGGCGG‑TG‑AATTTTTCCAGACAAATAC‑AAAAACGGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                           <  2:308395/239‑1 (MQ=255)

                                                                                                                                                                                    aGTTTGTGGCGG‑TG‑AATTTTTCCAGACAAATAA‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGAAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                           <  2:139719/239‑1 (MQ=255)

                                                                                                                                                                                     gTTTGTGGCGG‑AG‑AATTTTCCCAAAAAAATAA‑AAAAACTGGA‑GTTGCCATGATGAGA‑GAAAGTAATAAGCACC‑AGGAAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                           <  1:1420966/238‑1 (MQ=255)

                                                                                                                                                                                      agttgggggggggaaaTTTTCACAGAAAAAAAA‑AAAAAGTGGA‑GTTGCAAGGAGGAAG‑AAAAGAAAAAAGCAAA‑AGGCAGTAAAAAAGTGGAAAGAG‑ATGGTCGA‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                           <  2:540806/225‑1 (MQ=255)

                                                                                                                                                                                      agttggggggggaaaaTTTTTCCAGAAAAAAAC‑AAAAACGGGA‑GTTGCGATGATGATG‑AAAAGTAAAAAGCAAC‑AGGAAGTGAAAAATTGGACAGAG‑TGTGTCGC‑AAACTTAACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                           <  1:1644899/225‑1 (MQ=255)

                                                                                                                                                                                       tggtgGCGT‑AG‑AATATTACCGGAAAAAAAA‑AAAACGTGGA‑GTTGCAATGATGAAA‑AAAAGTAAAAACAAAC‑AGGCAGTGAAGAAGTGAACAAAG‑TTTGTGCT‑TAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGAtt                                                         <  1:642651/236‑1 (MQ=255)

                                                                                                                                                                                         gtgGCTG‑TG‑AATTTTTCCAGACAAATAC‑AAAAACTGGA‑GTTGCAAGGATGATC‑AAAAGAAATAAGAAAC‑AGGCATTAAAAAATTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAc                                                      <  2:1236633/239‑1 (MQ=255)

                                                                                                                                                                                           tgCGG‑TG‑AATTTTTCCAGACAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGa                                                       >  2:195466/2‑236 (MQ=255)

                                                                                                                                                                                                        ttaccaaaaaaaaac‑aaaaacTGGA‑GTGGCCATGATGAGG‑GAAAGAAATAAGCAAC‑AGGCAGTAAATAAGTTGACAGAG‑ATTGTCGC‑TACTTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                         <  2:1007273/230‑1 (MQ=255)

                                                                                                                                                                                                         ttacaaaaaaaaaaaaaaagCGGGA‑TTTGCGATGATGACC‑GAAAGAAAAAACAAAA‑AGGCAGTAAAAAAGTGGACAGAG‑ATGGTCGC‑AAATTTTCCCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCGGAATCGTcctg                                         <  2:1105139/224‑1 (MQ=255)

                                                                                                                                                                                                               aaaaaTAA‑AAAAACGGGA‑GTTGCGAGTATGAAC‑AAAAGAAATAAGCAAC‑AGGCAGTAAATAAGTTGACAGAG‑TTTGCCGC‑TATCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCa                                  <  1:195466/237‑1 (MQ=255)

                                                                                                                                                                                                                           aaCTGGA‑TTTGCAAGGATGGGC‑GAAAGAAAAAAGCAAC‑AGGCAGTAAATAAGTGGACAGAA‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                       <  2:417638/239‑1 (MQ=255)

                                                                                                                                                                                                                           aaCGGGA‑GTTCCCATGAGGAGC‑AAAAGTAAAAAGAACA‑AGGAAGTAAATAGGTGAACAGAG‑ATTGCCGT‑AAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                       <  1:652316/239‑1 (MQ=255)

                                                                                                                                                                                                                            aCTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGga                      >  2:1480569/1‑239 (MQ=255)

                                                                                                                                                                                                                               ggA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAt                   >  2:536291/1‑239 (MQ=255)

                                                                                                                                                                                                                               ggA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAt                   >  1:312214/1‑239 (MQ=255)

                                                                                                                                                                                                                               ggA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAt                   >  1:849426/1‑239 (MQ=255)

                                                                                                                                                                                                                                gA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAACCTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACACCATGCAAAAAGAGATTGAACTGAATCGTCCTGCCTGTCAGGACACCTGGGCGAtt                  >  1:842566/1‑239 (MQ=255)

                                                                                                                                                                                                                                      gCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGtt             >  1:1455343/1‑239 (MQ=255)

                                                                                                                                                                                                                                      gCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGtt             <  2:1584633/239‑1 (MQ=255)

                                                                                                                                                                                                                                                 c‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg  >  2:738429/1‑239 (MQ=255)

                                                                                                                                                                                                                                                 c‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGACGC‑TCACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGAAGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGCTTGACCTGAATCGTCCTGCCGGTCAGGACAGCGGGGAGATTATGTACTTCGTTAAAg  >  2:978475/1‑239 (MQ=255)

TAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGACAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAG  >  NC_000913/3206234‑3206707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`