BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,206,474	G→A	11.2%	S5N (AGT→AAT) ‡	ttdA →	L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,474	0	G	A	11.2%	223.8 / 4.7	110	S5N (AGT→AAT) ‡	ttdA	L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand): ref base G (40/52); new base A (10/3); total (50/60)
Fisher's exact test for biased strand distribution p-value = 3.60e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

CAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAG  >  NC_000913/3206241‑3206707

caacTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCAATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGtaat                                                                                                                                                                                                                                          <  1:1662194/235‑1 (MQ=255)

cAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGtaat                                                                                                                                                                                                                                          <  2:1373669/238‑1 (MQ=255)

  gCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑GG‑AATTTTTTCCGA‑CAAATAA‑AAAAAATGGA‑GTTGTCATGTTGGGT‑GAAAGAAAAAAg                                                                                                                                                                                                                                       >  2:1519060/1‑239 (MQ=255)

              cGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑GATTTTTTCAGA‑CAAATAC‑AAAAAACGGG‑GTTGGCCTGATGTGC‑GAAAGAAATAAAAAAAAGGCAggg                                                                                                                                                                                                                           >  2:550144/1‑237 (MQ=255)

              cGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAAA‑CAAATAC‑AAAAAATGGA‑GATGTCATGATTAGA‑GAAAAGAAAAAACAACAAGCAATg                                                                                                                                                                                                                           >  2:1580551/1‑239 (MQ=255)

                ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTTACA‑ACAAAAC‑AAAAAATGAA‑GTTTTCATGAGGAGC‑GAAAGGAATAAACAAAAGGCAGTGaa                                                                                                                                                                                                                         >  2:792084/1‑239 (MQ=255)

                ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AAATTTTTCTGA‑CAAAATA‑AAAAACAGGA‑GTTTGCATGGTTGGC‑GGAAGgaataaagaaaagggaaggaa                                                                                                                                                                                                                         >  1:417638/1‑224 (MQ=255)

                 tAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAACAGGCAGTGaa                                                                                                                                                                                                                         <  1:158652/238‑1 (MQ=255)

                    ttATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAACAGGCAGTGAATAAg                                                                                                                                                                                                                     <  1:542581/239‑1 (MQ=255)

                         cGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGGTTG‑AATTTTT‑CTGA‑CAAAAAA‑AAAAAATCGG‑GGTGCCCTTTTGTGG‑GATAGTAATAAACAACACGCAGTGAaaaaagtta                                                                                                                                                                                                                 >  2:778295/1‑233 (MQ=255)

                         cGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑GG‑AATTTTTTCAGACCAAAAAA‑AAAAAATGAG‑GTTG‑CATTGTGAGG‑GAAAATAAAAAACAAAAAGGAAGGAATAAGATGaa                                                                                                                                                                                                                >  1:693594/1‑238 (MQ=255)

                               tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgag‑a                                                                                                                                                                                                          <  1:1603904/238‑1 (MQ=255)

                                      aGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AAATTTTTCAGA‑AAAATAA‑AAAAAATGGA‑GGTGGCATGATGAGC‑GAAAATAATAAAAAACAAGACGTGAAAAAGATGACAGAG‑AATGACGC‑t                                                                                                                                                                                                 >  2:1556180/1‑239 (MQ=255)

                                        cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑TATTTTTTCAGA‑CAAATAC‑AAAAACAGGG‑GTTTGCACGATGAGC‑GAAAGTAATTAGCAAAAAACACAGAATAAATTTGCAGAG‑GTTGTCGC‑Taa                                                                                                                                                                                               >  2:1273840/1‑239 (MQ=255)

                                        cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTTAGA‑CAAATAA‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAACAGGCAGAGAATAAATTGACAGAG‑ATTGTCGC‑Taa                                                                                                                                                                                               >  2:1152676/1‑239 (MQ=255)

                                        cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑GG‑AATTTTTTCTTA‑CAAATAA‑AAAAAAATGG‑GTTGCCCTGATGTGG‑GAGAGGAATAAACAACAGCGAGGGTATAAGTTGAGAGAG‑GTTGTTGC‑Ta                                                                                                                                                                                                >  2:704777/1‑238 (MQ=255)

                                        cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑AG‑AAATTTTTCAGG‑CCAATAC‑AAAAAATGGA‑GGTTCCATTATGGGG‑GGAAGAAATAAACAACAGGCAGTGACAAAGATGACAGAA‑ATTGtgtccaa                                                                                                                                                                                                >  2:1185310/1‑233 (MQ=255)

                                                  ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCCGA‑CAAAAAC‑AAAAACTGGA‑GGTGGCATGATTAGC‑GGAAGAAATAAACAACAAGCAGTGAATAAAGTGACAGAG‑ATTGTTGC‑TAACTTTACCGc                                                                                                                                                                                      >  2:1069861/1‑238 (MQ=255)

                                                       aaaaCATTGCCATTCCCTGCTCTGGCGAAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑AAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGAAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑At                                                                                                                                                                               <  2:1430263/239‑1 (MQ=255)

                                                       aaaaCATGGCCATTCCCTTCCCTGGCGAAATTTTCATTCTTATTTCCACGTTGATTCACGAATTGTTAATTACATATCCAGGGTGGCAAAAAATATAAACCCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAAAAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAACGGGAAGTGAATAAGTTGACAGAG‑ATTGTCGT‑TAACTTTACCGCCATG‑At                                                                                                                                                                               <  2:573091/239‑1 (MQ=255)

                                                         aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTTCGT‑CAAAAAC‑AAAAAATGTA‑GTTGTCATGGTGAGC‑GAAAGTAATAAGAAAAAGGCAGTGAAAAAAATGGTAGAA‑ATTTTCTC‑CAACTTTTCCCCCCTG‑Agtt                                                                                                                                                                             >  1:850490/1‑239 (MQ=255)

                                                         aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCCCA‑AAAAAAA‑AAAAAATGGA‑GTTGCCATTATGAGC‑GGAAGTAATAAACAAAAAGCAGTGAATAAGATGACAGAG‑ATTGTCGC‑TAACTTTTCCGCCCTG‑Attt                                                                                                                                                                             >  2:860709/1‑239 (MQ=255)

                                                         aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAGA‑CAAATAA‑AAAAAATGGG‑GTTTGCCTGGTGAGG‑GAAAGTAAAAAAAAAACAGCAGTGAAAAAAGTGAAAGAG‑AGTGTGTC‑TAACttttaccccataaatt                                                                                                                                                                              >  1:881903/1‑226 (MQ=255)

                                                              tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTTCGA‑CAAAAAC‑AAAAACATGA‑GTTGCCATGATGAGA‑GGAAAGAATAAGCAAAAGGCAATGAATAAGATGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCttc                                                                                                                                                                         >  2:652316/1‑236 (MQ=255)

                                                              tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCCGA‑CAAAAAC‑AAAAAAATGG‑GTTGGCCTTGTTAGG‑GAAAGGAATAAAAAAAAGGCAGTGAATAAATTGACAGAG‑ATTGTTGC‑TAACTTTACCCCCCTG‑ATTTTTAc                                                                                                                                                                         >  2:1082752/1‑238 (MQ=255)

                                                                  tttCCCTTCTTTGGCTAAATGTTCATTCTTATTTCGGCGTTGATTCACGAACGGTTAATTAAATATTCACGGTGGAAAAAAATATAAAACCAC‑ATTTTGA‑GTGGTAGTTTGTGGGGT‑TG‑AATTTTACCAGA‑CAAATAC‑AAAAACTGGA‑GGGGCCATGAGGAGC‑GAAAGTAATAGGCAACAGGCGGTGAATAAGTTGAAAGAG‑ATTGTCCC‑TAACTTTACCGCCATG‑ATTTCTACCCGaa                                                                                                                                                                    <  1:1412054/238‑1 (MQ=255)

                                                                  aTTCCCTGCTCTGGAGAAATATTCATTCTTATTTGCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGAAAAAAATAAAAAACCAA‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TA‑AATTTTTCAAGA‑AAAATAA‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAACAGGAAGTAAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGaa                                                                                                                                                                    <  1:292034/239‑1 (MQ=255)

                                                                     cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAAA‑CAAAAAA‑CAAAAATGGA‑GTTTCCCTGAGTAGC‑GAAAAAAATAAACAACAGAAAGTGAATAAGTTGGAAGAG‑ATAGTCGA‑GAACTTTTCAGCCCAG‑CTTTATATCCGaaag                                                                                                                                                                  >  1:838519/1‑236 (MQ=255)

                                                                        tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AACTTTTTTCGA‑CACATAC‑AAAAACTCGA‑GTTGCCATGGTGAGC‑GGAAGTAAAAAAAAACACGAACTGAAAAATATGAAAGAG‑ATTGTTGA‑TAAATTTACCGCCAttagtttcatccctaagcctt                                                                                                                                                               >  1:800609/1‑219 (MQ=255)

                                                                          ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAGA‑CAAAAAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAACAAGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAAATTTTTCCCCCTG‑ATTTTTACCCCAATGCCtgat                                                                                                                                                            >  2:292034/1‑239 (MQ=255)

                                                                          ctctGGCGAAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAACCCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑AAAATAA‑AAAAACGGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCa                                                                                                                                                                                    <  2:577569/216‑1 (MQ=255)

                                                                           tcgggCGCAATATTCATTCTTATTTTCGAGTGAATTCACGAACTGTTAATTACATATCCAGGGTGGCAAAAAAAATAAACCCAC‑ATTTTTA‑GTGGTAGTTTGGGGGGG‑TA‑AATTTTTCCAGA‑CAAATAC‑AAAAACGGGA‑GTTGCCTGGAGGGGG‑GAAAGTAATAAGCAACAGGCAGTGAAAAAGTTGACGGAG‑ATTGTCGT‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCtgatg                                                                                                                                                           <  1:584548/236‑1 (MQ=255)

                                                                             tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAACCCAC‑ATTTTTA‑GTGGTATTTTGGGGCGG‑TG‑AATTTTTCCAAA‑CAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑AAAATTAATAAGCAACAGGAAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGAc                                                                                                                                                         <  2:422094/239‑1 (MQ=255)

                                                                              ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCTAA‑CAAATAC‑AAAAAATGGA‑GTTGTCATGATTAGT‑GAGAGTAAAAAAAAAACGACAATGAATAAATTGAAAGAG‑AATGTCGC‑TAACTTTAACGCCCTG‑ATTTTTATCCGAATGCCtgaggagg                                                                                                                                                        >  1:1584633/1‑237 (MQ=255)

                                                                               gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAGA‑CAAAAAC‑AAAAAATGGAGGTTGCC‑TGATGAGC‑GAAAGTAATAAGCAACAAGCAGAGAATAAGTTGACAGAG‑GTTGTCGC‑CAAATTTACCGCCCTG‑ATTTCTACCCGAATGCCTGATGACgt                                                                                                                                                       >  1:308395/1‑239 (MQ=255)

                                                                                cgcAATATTTATTTTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATTCACGGGGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGGGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACGGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAT‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACgt                                                                                                                                                       <  1:1612242/238‑1 (MQ=255)

                                                                                    atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑GATTTTTTCAAA‑CAAATAC‑AAAAAATGGA‑GTTTCCCTGATGAGG‑GAAAGTAATAAACAACAAGCAGAGAATAAATTGACAGAG‑ATTGTCGC‑TAAATTTACAGCCCTG‑ATTTCTaa                                                                                                                                                                         >  1:577569/1‑215 (MQ=255)

                                                                                             cTTATTTTCGTTTTGATTCACGACCTGTTAATTACATATTCACGGTGGAAAAAAAAATAAAACCAC‑ATTTTTA‑GTGGTGGTTTGGGGCGG‑TG‑AATTTTTCAAGA‑CAAATAA‑AAAAACTGGA‑GTTCCCATGATGAGC‑GAAAGTAATAAGCACCAGGCAGTAAATAATTTGCCAGAG‑ATTGTCTC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa                                                                                                                                         <  2:621385/239‑1 (MQ=255)

                                                                                               tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTTTGA‑CAAAAAC‑AAAAAAAGGA‑GTTGCCATGATGAGC‑GAAAGTAAAAAACAACACGCCGTGGATAAGGTTACCGAG‑ATTTTCGC‑TAACTTTACCCCCACG‑CTTTTTACCCCAAAGTCCGCTTGCGTGGTGGGTATACTaaaa                                                                                                                                       >  1:1313315/1‑239 (MQ=255)

                                                                                                aaagatgtgtaaaagagaaaggtGTTAATTACATATTCACGGTGGAAAAAAATAAAAAACCAC‑ATTTTTA‑GTGGTAGTTGGTGGCGG‑TG‑AATTTTTCCAGA‑AAAATAC‑AAAAACGGGA‑TTTGCCATGATGAGC‑GAAAGAAATAAGAAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                       <  1:985178/216‑1 (MQ=255)

                                                                                                     cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑AAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTa                                                                                                                                 <  2:448102/239‑1 (MQ=255)

                                                                                                          ggATTCACGAACTTTAAATTACATATTCAGGGTGGAAAAAAATAAAAAACCAC‑ATTTTGA‑GTGGTAGTTGGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACGGGA‑TTTGCCATGATGAGC‑GAAAGTAATAAGCAACAGGCAGTGAATAAGTGGACAGAG‑ATTGTCGC‑TAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGa                                                                                                                            <  1:1302414/238‑1 (MQ=255)

                                                                                                           gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAGA‑ACAATAA‑AAAAACTGGT‑GTTTCCATTATGAGG‑GGAAGTAATAAAGCACAGGCAGTGAATAAGTTTGCAGAG‑AATGTGGC‑TAACTTTAACGCCATG‑ATTTCTACCCGCAAGCCTTATGAATTGGGGGAGAAAATAAAACATCTAAAGTAt                                                                                                                           >  2:442683/1‑239 (MQ=255)

                                                                                                                      tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TGAAATTTTTCCA‑A‑CAAAAAA‑AAAAAATGGA‑GGTGGCATGGTTGAC‑GAAAATAAAAAACAAAAAGCAGGGGAAAAATTGATAGAG‑ATTGTCGCTTACCTTTAAC‑CCATA‑ATTTTTTCCCGAACAGCTGATGACGGGGGGGGTTAAATAAAAAAAATAAAGGAAAGCGAAAcca                                                                                                                 >  1:1021539/1‑236 (MQ=255)

                                                                                                                      tGTTAATTAAATATTCACGGTGGCAAAAAATAAAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                       <  2:389060/216‑1 (MQ=255)

                                                                                                                      tGTTAATGACATATTCACGGTGGAAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAACAGGGAGGGAATAAGTTGACAGAG‑ATGGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACgtgg                                                                                                                                                     <  2:371281/202‑1 (MQ=255)

                                                                                                                        ttAATTACATATTCGCGGTGGAAAAAAATAAAAACCCAA‑ATTTTAA‑TGGGTATTTTGGGGGGG‑GA‑AATTTTTCAAAA‑AAAAAAC‑AAAAACGGGA‑GTTGCAATGATGAGC‑AAAAGAAATAAGCAACAGGCAGTGAAAAAGTTGAAAGAG‑TTGGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAcgtcgt                                                                                                              <  1:1082752/239‑1 (MQ=255)

                                                                                                                          aaTTATAAATTAGCGGTGG‑AAAAAAAAAAAAACCACAATTGTAA‑GGGGTATTTTGTGGCGG‑TA‑AATTTGTCCAGA‑AAAATAA‑AAAAGTAGAA‑GTGGCCAGTATGAGA‑GAAAGAAAAAAACAACAGCCTGTAAAAACGTTAACAGCG‑ATCGTCTC‑AACCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGtcgtcg                                                                                                            <  1:1273840/239‑1 (MQ=255)

                                                                                                                                atatTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCCGA‑CAAAAAC‑AAAAACTGGA‑GTTTCCCTTTTGTGC‑GGAAGTAATAAACCACCGGCAGTGAAAAAATTGGCAGAG‑ATTGTTGC‑TAAATTTACCGCCCTG‑ATTTTTTCCCGAAATCCTTGTTACGCGGTGGATAAAATAAAAAAACTAAAAGactgtctcttataaccctctccc                                                                                                      >  2:985178/1‑217 (MQ=255)

                                                                                                                                atatTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAGA‑CAAAAAA‑AAAAAATGGG‑GTTGGCATGATGAGC‑GAAAATAATAAAGAACAAGCAGGGAATAAATTGACAGAGAATTGT‑GC‑CAAATTTACCGCCCTG‑ATTTTTAACCGGATGCCTGATGACGGGGGGGGTAAACTAAAAAAGCTAAAAGAAGCCGAAACCTCGCCGATgggg                                                                                                      >  2:1707341/1‑239 (MQ=255)

                                                                                                                                 ttttagaggtgcaaaaaaaGAAAAACCCAC‑ATTTTTA‑GTTGTTGTTGGGGGCGG‑TA‑AATTTTCCCAGA‑AAAATAC‑AAAAAATGAA‑TTTGCATTGATGAGA‑GAAATTAATAAGCAACAGGCGGTGAATAAGTTGACAGAG‑ATTGTCGT‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                     <  2:1406998/226‑1 (MQ=255)

                                                                                                                                 tatgcgcGGTGGCAAAAAATAAAAAACCAA‑ATTGTTA‑GTGGTAGGTTGGGGGGG‑TG‑AATTTTCCAAGA‑CAAAAAC‑AAAAACTGGG‑GTGGCAATGAGGAGC‑AAAAAAAATAAGCAACAGGCAGTGAATAATTGAACAGAG‑ATTGTGGT‑AAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                     <  1:77868/233‑1 (MQ=255)

                                                                                                                                 tatTCACGGTGGAAAAAAATAAAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAAA‑AAAATAA‑AAAAAGTGGA‑GTGGCCATGATGAGC‑AAAATGAAAAAGCAACAGGCAGTGAATAAGTTGAAAGGT‑ATTGGCGC‑TAATTTAACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                     <  2:1589960/239‑1 (MQ=255)

                                                                                                                                 atgtctgtggggaaaaaaaaaaaaaACCCC‑GTTTGTG‑GTGGTAGGTTGGGGCGG‑TG‑AATTTTTCCAGA‑CAAATAA‑AAAAACTGGA‑GTGCCCAGCAAGGGA‑AAAAGAAAGAAGCAACAGGCAGTGAAAAAGTTGAGAGAG‑ATTGCCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                     <  1:499646/226‑1 (MQ=255)

                                                                                                                                  atTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCTGA‑CAAAAACAAAAAACTGG‑‑GTTGGCCTGATGTGC‑GAAAGTAAAAAACAACAAGCAAGGAATAAATTGACAGAG‑ATTGTCGC‑TAAATTTAACGCCATG‑AATTTTTCCCGAATGCCTGTTGACGTGGTGGGTAAAATAAAACAGCTAAAGGATGCCCGAACGTCGTCGATggggga                                                                                                    >  2:1625485/1‑237 (MQ=255)

                                                                                                                                  atTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAGA‑AAAAAAA‑AAAAAATGGA‑GGTGTCATGTTGAGC‑GAAAAGAATAAAAAAAAGGCAAGGAAAAAGTTGACAGAG‑AGTTTTGC‑TAAATTTTACGCCCTG‑ATTTCTACCCGAATGCCTGTTAACGTGGGGGATAAAATAAAAAAGCAAAAAGAAAACCAAAACACGTCGATGGGGaa                                                                                                    >  1:1027860/1‑239 (MQ=255)

                                                                                                                                  atTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑CG‑AAATTTTTCAAA‑CAAAAAC‑AAAAAAAGGA‑GTTGCCACGGTGGGG‑GGAAAGAAAAAACAAAAAGCAAAGAAAAAATTGACAGAG‑AATGTCGC‑TAAATTTTCCCCCCTGAATTTTTAACC‑AAAGCCTGGTGACGTGGGGGGTAAACTTAAAAAAATCAAAGATACCGCAAagtcgtgcattgggga                                                                                                    >  1:1236633/1‑229 (MQ=255)

                                                                                                                                    tCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCCGA‑CAAATAC‑AAAAAATTGA‑GTTTCCCTGATGAGC‑GGAAAAAATAAACAACAAGCAGTGAATAAGTTGACAGAG‑ATTGTTGC‑TCACCTTACCGCCATG‑ATTTTTTCCCCAATGCCTGATGAAGTGGGGGGTAAACTAAAACAGCTAAAAGAAGCCCAAACATCGTCGATGGGGgaaa                                                                                                  >  2:1420966/1‑239 (MQ=255)

                                                                                                                                      aCGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAGA‑CAAAAAA‑AAAAAAATGA‑GTTGCCCTGATGAGC‑GAAAGTAATAAACAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTTCCCGAATGCCTGATGACGTGGTGGATAAACTAAAAAAACTAAAgg                                                                                                                             >  2:965403/1‑210 (MQ=255)

                                                                                                                                        ggTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑AAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                              <  2:164378/239‑1 (MQ=255)

                                                                                                                                         ggggCAAAAAAGAAAAAACCAT‑ATGTTTG‑GGGGTAGTTTGTGGGGG‑TG‑ATTTTTGCAAGA‑AAAATAC‑AAAAACTGGT‑GTTGCAATGATGAGC‑AAAAGTAATAAGCAACGTGGAGTTAATAAGTGGACAGAG‑ATTGCCGT‑TACCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGAAAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                              <  1:1063215/236‑1 (MQ=255)

                                                                                                                                         gTGGAAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACGGGA‑GTGGCCATGATGAGC‑GAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                              <  2:838519/238‑1 (MQ=255)

                                                                                                                                         gTGGAAAAAAAAATAAAACCA‑‑ATTTTTAGGTGGTAGTTGGGGGCGG‑TA‑AATTTTTCCAGA‑CAAATAC‑AAAAATTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAACAACAGGCATTAAATAATTTAAAAGAT‑TTTGTCGC‑TATCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                              <  1:458088/238‑1 (MQ=255)

                                                                                                                                          tgtaaaaaaaTAAAAACCTAA‑ATTTTTA‑GTGGTGTTTTGTGGGGG‑AA‑AATTTTCCAAAA‑AAAATAC‑AAAAACGAGA‑GTGCCTATGA‑GAGCAAAAAGTAATAAGCAAAAGACAGAGAATAAGTTGACGGAG‑ATTGTCGC‑AAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACGAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATc                                                                                             <  1:1313660/234‑1 (MQ=255)

                                                                                                                                             aaaaaaaTATAAAACCAC‑ATTTTTA‑GTGGTGGTTTGTGGGGG‑TG‑AATTTTTCCAGA‑AAAATAC‑AAAAACGGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGT‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTAc                                                                                          <  2:693594/237‑1 (MQ=255)

                                                                                                                                               aaaaaTATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAGA‑CAAAAAA‑AAAAAATGGA‑GTTGCCATGATGAGG‑GAAAGTAATAAACAACAGACAGTGAATAAGATGACAGAG‑ATTGTCGC‑TAAATTTTTCGCCCCG‑ATTTCTACCCGAATGCCTGCTGAGGTGGGGGGTAAACTAAAAAAGCTAAAGGATGCCGAAACGTCGTCGCTGGGGGAAATTATCTAccct                                                                                       >  2:78551/1‑237 (MQ=255)

                                                                                                                                                  aaaataAAACCTC‑ATTTTAC‑TTGTAAGTTTGGGGCGG‑TG‑ATTTTTTCAAAA‑AAAAAAA‑AAAACCTGGA‑GTTGACTTAAGTGGA‑GAACATAATAAACACCAGGAAGTGAATAAGTTGAAAGAA‑TTTGTCCT‑TAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                    <  2:519224/236‑1 (MQ=255)

                                                                                                                                                         aaCCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTTAAA‑CAAAAAA‑AAAAAATGGA‑GTTTGCATTATGGGG‑GGAAGAAATAAAAAACAAACAGAGAAAAAATTTACAAAG‑ATTGTCGC‑TAAACTTACCCCCACG‑ATTTTTTCCCCAAAGCCCCGTGACGGGGGGGGTAAAACAAAAAAACCAAAAGATGTCCAAACATCGCGGGTGGGGGAAAATATCTAACaacagcagttcg                                                                             >  1:139719/1‑228 (MQ=255)

                                                                                                                                                          cccAC‑ATTTTTA‑GTGGTATTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGa                                                                            <  2:1609537/238‑1 (MQ=255)

                                                                                                                                                           ccAA‑ATTTGTA‑GTGGTTGTTTGTGGCGG‑TG‑AATTTTTACAGA‑CAAAAAA‑AAAAACGGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGac                                                                           <  1:1185310/239‑1 (MQ=255)

                                                                                                                                                             ac‑aTATTGA‑GTGGTAGGTGAGGGGGG‑TG‑AATTTTTAAAGA‑AAAAAAA‑AAAAACGGGT‑GTTCCCATGATGAGC‑GAAATAAATAAGAAAGAGCCAGTGAAAAAGTTGAAAGAG‑ATTGTGGG‑AAACTTTCCCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGacaa                                                                         <  1:792084/239‑1 (MQ=255)

                                                                                                                                                              c‑aTTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AAATTTTTCAAA‑CAAAAAA‑AAAAAATGGA‑GTTGGCATGATGAGC‑GAAAGTAAAAAACAAAAAGCAGGGAATAAATTGACAGAG‑ATTGTCGC‑TAACTTTAAAGCCCTA‑ATTTCTACCCGAATGCCTGATGACGGGGGGGGTAAACTAAAAAAGCTAAAGGATGCCGAAAAGTCGTCGATGGGGAGAAATATCTACCCTAAGATGTTCGacaaa                                                                        >  1:1609537/1‑238 (MQ=255)

                                                                                                                                                                    ttA‑GTGGTAGTTTGGGGCGG‑TA‑AATTTTTCCAGA‑CAAATAA‑AAAAACTGGA‑GTTGCCATGATGAGC‑AAAAGTAAAAAGCAACGGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCa                                                                   <  2:850490/239‑1 (MQ=255)

                                                                                                                                                                             attttagggCTG‑TA‑AATTTTAACAGA‑AAAATAC‑AAAATCGTGA‑GTTGCAGTGATGAGG‑AAAGGTAGTAATCAACAGCAAGTGAATAAGTTGCCAGAT‑TTTGTAGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGGGGTGGATAAACTAAAACAGCTAAAGGATGCCTAACCGGCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                           <  2:1229731/232‑1 (MQ=255)

                                                                                                                                                                             aGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAACCTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                           <  2:396519/239‑1 (MQ=255)

                                                                                                                                                                             aGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACGGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                           <  2:308395/239‑1 (MQ=255)

                                                                                                                                                                             aGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAA‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAACAGGAAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                           <  2:139719/239‑1 (MQ=255)

                                                                                                                                                                              gTTTGTGGCGG‑AG‑AATTTTCCCAAA‑AAAATAA‑AAAAACTGGA‑GTTGCCATGATGAGA‑GAAAGTAATAAGCACCAGGAAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                           <  1:1420966/238‑1 (MQ=255)

                                                                                                                                                                               agttgggggggggaaaTTTTCACAGA‑AAAAAAA‑AAAAAGTGGA‑GTTGCAAGGAGGAAG‑AAAAGAAAAAAGCAAAAGGCAGTAAAAAAGTGGAAAGAG‑ATGGTCGA‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                           <  2:540806/225‑1 (MQ=255)

                                                                                                                                                                               agttggggggggaaaaTTTTTCCAGA‑AAAAAAC‑AAAAACGGGA‑GTTGCGATGATGATG‑AAAAGTAAAAAGCAACAGGAAGTGAAAAATTGGACAGAG‑TGTGTCGC‑AAACTTAACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                           <  1:1644899/225‑1 (MQ=255)

                                                                                                                                                                                tggtgGCGT‑AG‑AATATTACCGGA‑AAAAAAA‑AAAACGTGGA‑GTTGCAATGATGAAA‑AAAAGTAAAAACAAACAGGCAGTGAAGAAGTGAACAAAG‑TTTGTGCT‑TAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGAtt                                                         <  1:642651/236‑1 (MQ=255)

                                                                                                                                                                                  gtgGCTG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACTGGA‑GTTGCAAGGATGATC‑AAAAGAAATAAGAAACAGGCATTAAAAAATTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAc                                                      <  2:1236633/239‑1 (MQ=255)

                                                                                                                                                                                    tgCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGa                                                       >  2:195466/2‑236 (MQ=255)

                                                                                                                                                                                                  ttaccaaaaaaaaac‑aaaaacTGGA‑GTGGCCATGATGAGG‑GAAAGAAATAAGCAACAGGCAGTAAATAAGTTGACAGAG‑ATTGTCGC‑TACTTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                         <  2:1007273/230‑1 (MQ=255)

                                                                                                                                                                                                   ttacaaaaaaaaaaaaaaagCGGGA‑TTTGCGATGATGACC‑GAAAGAAAAAACAAAAAGGCAGTAAAAAAGTGGACAGAG‑ATGGTCGC‑AAATTTTCCCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCGGAATCGTcctg                                         <  2:1105139/224‑1 (MQ=255)

                                                                                                                                                                                                         aaaaaTAA‑AAAAACGGGA‑GTTGCGAGTATGAAC‑AAAAGAAATAAGCAACAGGCAGTAAATAAGTTGACAGAG‑TTTGCCGC‑TATCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCa                                  <  1:195466/237‑1 (MQ=255)

                                                                                                                                                                                                                     aagtGGA‑GTTGCCATGAGGTGA‑GAAAATAATGGCCCACAGGGAGTGAAAAAGTTGACGGTA‑TTTGATCA‑GAGCTTTACCGCCATG‑ATTTCTCCCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAGCATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                       <  2:1418311/236‑1 (MQ=255)

                                                                                                                                                                                                                     aaCTGGA‑TTTGCAAGGATGGGC‑GAAAGAAAAAAGCAACAGGCAGTAAATAAGTGGACAGAA‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                       <  2:417638/239‑1 (MQ=255)

                                                                                                                                                                                                                     aaCGGGA‑GTTCCCATGAGGAGC‑AAAAGTAAAAAGAACAAGGAAGTAAATAGGTGAACAGAG‑ATTGCCGT‑AAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                       <  1:652316/239‑1 (MQ=255)

                                                                                                                                                                                                                      aCTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGga                      >  2:1480569/1‑239 (MQ=255)

                                                                                                                                                                                                                         ggA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAt                   >  2:536291/1‑239 (MQ=255)

                                                                                                                                                                                                                         ggA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAt                   >  1:312214/1‑239 (MQ=255)

                                                                                                                                                                                                                          gA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAACCTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACACCATGCAAAAAGAGATTGAACTGAATCGTCCTGCCTGTCAGGACACCTGGGCGAtt                  >  1:842566/1‑239 (MQ=255)

                                                                                                                                                                                                                                gCCATGATGAGC‑GAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGtt             >  1:1455343/1‑239 (MQ=255)

                                                                                                                                                                                                                                gCCATGATGAGC‑GAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGtt             <  2:1584633/239‑1 (MQ=255)

                                                                                                                                                                                                                                          gagaaaaGTAAAAAGCAAAAGGCAGTAAATAAGTTGACAGAG‑ATTGCGGC‑AACCTTAACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGtt      <  1:1556180/233‑1 (MQ=255)

                                                                                                                                                                                                                                           c‑GAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGa                                                                                   >  2:1076207/1‑158 (MQ=255)

                                                                                                                                                                                                                                           c‑GAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg  >  2:738429/1‑239 (MQ=255)

                                                                                                                                                                                                                                           c‑GAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGACGC‑TCACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGAAGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGCTTGACCTGAATCGTCCTGCCGGTCAGGACAGCGGGGAGATTATGTACTTCGTTAAAg  >  2:978475/1‑239 (MQ=255)

                                                                                                                                                                                                                                             aaaaGTAATAAGCAACAGGAAGTAAATAAGGTGACAGAG‑TTTGTCGC‑TAACTTTGCCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTaaa   <  2:68886/236‑1 (MQ=255)

CAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAG  >  NC_000913/3206241‑3206707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`