Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,485 C→A 16.1% Q9K (CAG→AAG)  ttdA → L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4850CA16.1% 222.0 / 9.2 108Q9K (CAG→AAG) ttdAL‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (34/54);  new base A (14/5);  total (48/60)
Fisher's exact test for biased strand distribution p-value = 9.63e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

CGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCC  >  NC_000913/3206255‑3206723
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 
cGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑GATTTTTTCAGA‑CAAATAC‑AAAAAACGGG‑GTTGGCCTGATGTGC‑GAAAGAAATAAAAAAA‑AGGCAggg                                                                                                                                                                                                                                          >  2:550144/1‑237 (MQ=255)
cGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAAA‑CAAATAC‑AAAAAATGGA‑GATGTCATGATTAGA‑GAAAAGAAAAAACAAC‑AAGCAATg                                                                                                                                                                                                                                          >  2:1580551/1‑239 (MQ=255)
  ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTTACA‑ACAAAAC‑AAAAAATGAA‑GTTTTCATGAGGAGC‑GAAAGGAATAAACAAA‑AGGCAGTGaa                                                                                                                                                                                                                                        >  2:792084/1‑239 (MQ=255)
   tAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGaa                                                                                                                                                                                                                                        <  1:158652/238‑1 (MQ=255)
      ttATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAg                                                                                                                                                                                                                                    <  1:542581/239‑1 (MQ=255)
           cGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGGTTG‑AATTTTT‑CTGA‑CAAAAAA‑AAAAAATCGG‑GGTGCCCTTTTGTGG‑GATAGTAATAAACAAC‑ACGCAGTGAaaaaagtta                                                                                                                                                                                                                                >  2:778295/1‑233 (MQ=255)
           cGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑GG‑AATTTTTTCAGACCAAAAAA‑AAAAAATGAG‑GTTG‑CATTGTGAGG‑GAAAATAAAAAACAAA‑AAGGAAGGAATAAGATGaa                                                                                                                                                                                                                               >  1:693594/1‑238 (MQ=255)
                 tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAgag‑a                                                                                                                                                                                                                         <  1:1603904/238‑1 (MQ=255)
                        aGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AAATTTTTCAGA‑AAAATAA‑AAAAAATGGA‑GGTGGCATGATGAGC‑GAAAATAATAAAAAAC‑AAGACGTGAAAAAGATGACAGAG‑AATGACGC‑t                                                                                                                                                                                                                >  2:1556180/1‑239 (MQ=255)
                          cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑TATTTTTTCAGA‑CAAATAC‑AAAAACAGGG‑GTTTGCACGATGAGC‑GAAAGTAATTAGCAAA‑AAACACAGAATAAATTTGCAGAG‑GTTGTCGC‑Taa                                                                                                                                                                                                              >  2:1273840/1‑239 (MQ=255)
                          cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTTAGA‑CAAATAA‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGAGAATAAATTGACAGAG‑ATTGTCGC‑Taa                                                                                                                                                                                                              >  2:1152676/1‑239 (MQ=255)
                          cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑GG‑AATTTTTTCTTA‑CAAATAA‑AAAAAAATGG‑GTTGCCCTGATGTGG‑GAGAGGAATAAACAAC‑AGCGAGGGTATAAGTTGAGAGAG‑GTTGTTGC‑Ta                                                                                                                                                                                                               >  2:704777/1‑238 (MQ=255)
                          cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑AG‑AAATTTTTCAGG‑CCAATAC‑AAAAAATGGA‑GGTTCCATTATGGGG‑GGAAGAAATAAACAAC‑AGGCAGTGACAAAGATGACAGAA‑ATTGtgtccaa                                                                                                                                                                                                               >  2:1185310/1‑233 (MQ=255)
                             agtttttttCGCAAAACATTGCCATCCCCTGTTCTGGCGAAATATTTATTCTTATTTTCGGTTTGATTCACGAACTGTTAATAACATATTCGGGGTGAAAAAAAATAAAAAACCAC‑ATTTTTA‑GTGGTAGTTTGGGGGGG‑TA‑AATTTTTCCAGA‑CAAATAC‑AAAAACGGGA‑GTTGCCATGATGAGC‑AAAATAAATAAGCAAC‑AGGCAGTAAATAAGTTGACAGAG‑ATTGTCGC‑TAACtt                                                                                                                                                                                                           <  1:550144/233‑1 (MQ=255)
                                    ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCCGA‑CAAAAAC‑AAAAACTGGA‑GGTGGCATGATTAGC‑GGAAGAAATAAACAAC‑AAGCAGTGAATAAAGTGACAGAG‑ATTGTTGC‑TAACTTTACCGc                                                                                                                                                                                                     >  2:1069861/1‑238 (MQ=255)
                                         aaaaCATTGCCATTCCCTGCTCTGGCGAAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑AAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGAAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGAt                                                                                                                                                                                               <  2:1430263/239‑1 (MQ=255)
                                         aaaaCATGGCCATTCCCTTCCCTGGCGAAATTTTCATTCTTATTTCCACGTTGATTCACGAATTGTTAATTACATATCCAGGGTGGCAAAAAATATAAACCCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAAAAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑GGGAAGTGAATAAGTTGACAGAG‑ATTGTCGT‑TAACTTTACCGCCATGAt                                                                                                                                                                                               <  2:573091/239‑1 (MQ=255)
                                           aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCCCA‑AAAAAAA‑AAAAAATGGA‑GTTGCCATTATGAGC‑GGAAGTAATAAACAAA‑AAGCAGTGAATAAGATGACAGAG‑ATTGTCGC‑TAACTTTTCCGCCCTGAttt                                                                                                                                                                                             >  2:860709/1‑239 (MQ=255)
                                           aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAGA‑CAAATAA‑AAAAAATGGG‑GTTTGCCTGGTGAGG‑GAAAGTAAAAAAAAAA‑CAGCAGTGAAAAAAGTGAAAGAG‑AGTGTGTC‑TAACttttaccccataaatt                                                                                                                                                                                             >  1:881903/1‑226 (MQ=255)
                                                tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTTCGA‑CAAAAAC‑AAAAACATGA‑GTTGCCATGATGAGA‑GGAAAGAATAAGCAAA‑AGGCAATGAATAAGATGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCttc                                                                                                                                                                                         >  2:652316/1‑236 (MQ=255)
                                                tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCCGA‑CAAAAAC‑AAAAAAATGG‑GTTGGCCTTGTTAGG‑GAAAGGAATAAAAAAA‑AGGCAGTGAATAAATTGACAGAG‑ATTGTTGC‑TAACTTTACCCCCCTGATTTTTAc                                                                                                                                                                                         >  2:1082752/1‑238 (MQ=255)
                                                   cATTCCCTGCTCTGACTAAATATTCTTTCTTTTTTTCGAGTTGATTCACGAACTTTTAATTACATTTTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCACA‑AAAATAC‑AAAAACGGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAATTTAACAGAG‑ATTGTCTT‑TAACTTTACCGCCATGATTTCTACCCGa                                                                                                                                                                                     <  1:1350825/239‑1 (MQ=255)
                                                    tttCCCTTCTTTGGCTAAATGTTCATTCTTATTTCGGCGTTGATTCACGAACGGTTAATTAAATATTCACGGTGGAAAAAAATATAAAACCAC‑ATTTTGA‑GTGGTAGTTTGTGGGGT‑TG‑AATTTTACCAGA‑CAAATAC‑AAAAACTGGA‑GGGGCCATGAGGAGC‑GAAAGTAATAGGCAAC‑AGGCGGTGAATAAGTTGAAAGAG‑ATTGTCCC‑TAACTTTACCGCCATGATTTCTACCCGaa                                                                                                                                                                                    <  1:1412054/238‑1 (MQ=255)
                                                    aTTCCCTGCTCTGGCGAAATATTCATTCTTATTTTCGCGTTGATTCCCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGTTAGTTTGTGGCGT‑TA‑AATTTTTCCAGA‑CAAATAC‑AAAAACTGGA‑GTTGCCATGAAGAGC‑GAAAGTAATAAGCAAC‑GGGCAGTGAATAAGTTAACAGAG‑ATTGTCCC‑TAACTTTACCGCCATGATTTCTACCCGaa                                                                                                                                                                                    <  2:1501440/239‑1 (MQ=255)
                                                    aTTCCCTGCTCTGGAGAAATATTCATTCTTATTTGCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGAAAAAAATAAAAAACCAA‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TA‑AATTTTTCAAGA‑AAAATAA‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGAAGTAAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGaa                                                                                                                                                                                    <  1:292034/239‑1 (MQ=255)
                                                       cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAAA‑CAAAAAA‑CAAAAATGGA‑GTTTCCCTGAGTAGC‑GAAAAAAATAAACAAC‑AGAAAGTGAATAAGTTGGAAGAG‑ATAGTCGA‑GAACTTTTCAGCCCAGCTTTATATCCGaaag                                                                                                                                                                                  >  1:838519/1‑236 (MQ=255)
                                                       cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑t                                                                                                                                                                                                                <  2:529707/208‑1 (MQ=255)
                                                          tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AACTTTTTTCGA‑CACATAC‑AAAAACTCGA‑GTTGCCATGGTGAGC‑GGAAGTAAAAAAAAAC‑ACGAACTGAAAAATATGAAAGAG‑ATTGTTGA‑TAAATTTACCGCCAttagtttcatccctaagcctt                                                                                                                                                                              >  1:800609/1‑219 (MQ=255)
                                                            ctctGGCGAAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAACCCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑AAAATAA‑AAAAACGGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCa                                                                                                                                                                                                   <  2:577569/216‑1 (MQ=255)
                                                             tcgggCGCAATATTCATTCTTATTTTCGAGTGAATTCACGAACTGTTAATTACATATCCAGGGTGGCAAAAAAAATAAACCCAC‑ATTTTTA‑GTGGTAGTTTGGGGGGG‑TA‑AATTTTTCCAGA‑CAAATAC‑AAAAACGGGA‑GTTGCCTGGAGGGGG‑GAAAGTAATAAGCAAC‑AGGCAGTGAAAAAGTTGACGGAG‑ATTGTCGT‑TAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg                                                                                                                                                                           <  1:584548/236‑1 (MQ=255)
                                                               tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAACCCAC‑ATTTTTA‑GTGGTATTTTGGGGCGG‑TG‑AATTTTTCCAAA‑CAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑AAAATTAATAAGCAAC‑AGGAAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGAc                                                                                                                                                                         <  2:422094/239‑1 (MQ=255)
                                                                ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCTAA‑CAAATAC‑AAAAAATGGA‑GTTGTCATGATTAGT‑GAGAGTAAAAAAAAAA‑CGACAATGAATAAATTGAAAGAG‑AATGTCGC‑TAACTTTAACGCCCTGATTTTTATCCGAATGCCtgaggagg                                                                                                                                                                        >  1:1584633/1‑237 (MQ=255)
                                                                 gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTTATA‑AAAAAAA‑AAAAAATGGA‑GGTGGCCTGGTGAGC‑GGAAGTAATAAACAAC‑AGGGAAGGAATAAATTGACAAGG‑ATTGTCGC‑TAACTTTTCCCCCCTGATTTCTTCCCCAATGTCttgtggcgt                                                                                                                                                                       >  1:164378/1‑231 (MQ=255)
                                                                 gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAGA‑CAAAAAC‑AAAAAATGGAGGTTGCC‑TGATGAGC‑GAAAGTAATAAGCAAC‑AAGCAGAGAATAAGTTGACAGAG‑GTTGTCGC‑CAAATTTACCGCCCTGATTTCTACCCGAATGCCTGATGACgt                                                                                                                                                                       >  1:308395/1‑239 (MQ=255)
                                                                  cgcAATATTTATTTTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATTCACGGGGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGGGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACGGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAT‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgt                                                                                                                                                                       <  1:1612242/238‑1 (MQ=255)
                                                                      atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑GATTTTTTCAAA‑CAAATAC‑AAAAAATGGA‑GTTTCCCTGATGAGG‑GAAAGTAATAAACAAC‑AAGCAGAGAATAAATTGACAGAG‑ATTGTCGC‑TAAATTTACAGCCCTGATTTCTaa                                                                                                                                                                                         >  1:577569/1‑215 (MQ=255)
                                                                                 tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTTTGA‑CAAAAAC‑AAAAAAAGGA‑GTTGCCATGATGAGC‑GAAAGTAAAAAACAAC‑ACGCCGTGGATAAGGTTACCGAG‑ATTTTCGC‑TAACTTTACCCCCACGCTTTTTACCCCAAAGTCCGCTTGCGTGGTGGGTATACTaaaa                                                                                                                                                       >  1:1313315/1‑239 (MQ=255)
                                                                                  aaagatgtgtaaaagagaaaggtGTTAATTACATATTCACGGTGGAAAAAAATAAAAAACCAC‑ATTTTTA‑GTGGTAGTTGGTGGCGG‑TG‑AATTTTTCCAGA‑AAAATAC‑AAAAACGGGA‑TTTGCCATGATGAGC‑GAAAGAAATAAGAAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                       <  1:985178/216‑1 (MQ=255)
                                                                                            ggATTCACGAACTTTAAATTACATATTCAGGGTGGAAAAAAATAAAAAACCAC‑ATTTTGA‑GTGGTAGTTGGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACGGGA‑TTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTGGACAGAG‑ATTGTCGC‑TAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGa                                                                                                                                            <  1:1302414/238‑1 (MQ=255)
                                                                                             gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAGA‑ACAATAA‑AAAAACTGGT‑GTTTCCATTATGAGG‑GGAAGTAATAAAGCAC‑AGGCAGTGAATAAGTTTGCAGAG‑AATGTGGC‑TAACTTTAACGCCATGATTTCTACCCGCAAGCCTTATGAATTGGGGGAGAAAATAAAACATCTAAAGTAt                                                                                                                                           >  2:442683/1‑239 (MQ=255)
                                                                                                  aCGAATTTTAAATTACATTATCACGGTGCCAAAAAAAAGAAACCCAC‑ATTTTTA‑GTGGGAGTTTGTGGCGG‑AG‑AATTTTTCCAGA‑AAAATTG‑AAAAACGGTA‑GTTGCCTTGAGGAGC‑GAAAGTAA‑AAGCCACTGGGCAGTAAATAAGTTGATAGTG‑TTTGTCGC‑TACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGa                                                                                                                                      <  2:1224552/239‑1 (MQ=255)
                                                                                                        tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TGAAATTTTTCCA‑A‑CAAAAAA‑AAAAAATGGA‑GGTGGCATGGTTGAC‑GAAAATAAAAAACAAA‑AAGCAGGGGAAAAATTGATAGAG‑ATTGTCGCTTACCTTTAAC‑CCATAATTTTTTCCCGAACAGCTGATGACGGGGGGGGTTAAATAAAAAAAATAAAGGAAAGCGAAAcca                                                                                                                                 >  1:1021539/1‑236 (MQ=255)
                                                                                                        tGTTAATTAAATATTCACGGTGGCAAAAAATAAAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                       <  2:389060/216‑1 (MQ=255)
                                                                                                        tGTTAATGACATATTCACGGTGGAAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGGAGGGAATAAGTTGACAGAG‑ATGGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtgg                                                                                                                                                                     <  2:371281/202‑1 (MQ=255)
                                                                                                          ttAATTACATATTCGCGGTGGAAAAAAATAAAAACCCAA‑ATTTTAA‑TGGGTATTTTGGGGGGG‑GA‑AATTTTTCAAAA‑AAAAAAC‑AAAAACGGGA‑GTTGCAATGATGAGC‑AAAAGAAATAAGCAAC‑AGGCAGTGAAAAAGTTGAAAGAG‑TTGGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAcgtcgt                                                                                                                              <  1:1082752/239‑1 (MQ=255)
                                                                                                            aaTTATAAATTAGCGGTGG‑AAAAAAAAAAAAACCACAATTGTAA‑GGGGTATTTTGTGGCGG‑TA‑AATTTGTCCAGA‑AAAATAA‑AAAAGTAGAA‑GTGGCCAGTATGAGA‑GAAAGAAAAAAACAAC‑AGCCTGTAAAAACGTTAACAGCG‑ATCGTCTC‑AACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGtcgtcg                                                                                                                            <  1:1273840/239‑1 (MQ=255)
                                                                                                                  atatTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCCGA‑CAAAAAC‑AAAAACTGGA‑GTTTCCCTTTTGTGC‑GGAAGTAATAAACCAC‑CGGCAGTGAAAAAATTGGCAGAG‑ATTGTTGC‑TAAATTTACCGCCCTGATTTTTTCCCGAAATCCTTGTTACGCGGTGGATAAAATAAAAAAACTAAAAGactgtctcttataaccctctccc                                                                                                                      >  2:985178/1‑217 (MQ=255)
                                                                                                                  atatTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAGA‑CAAAAAA‑AAAAAATGGG‑GTTGGCATGATGAGC‑GAAAATAATAAAGAAC‑AAGCAGGGAATAAATTGACAGAGAATTGT‑GC‑CAAATTTACCGCCCTGATTTTTAACCGGATGCCTGATGACGGGGGGGGTAAACTAAAAAAGCTAAAAGAAGCCGAAACCTCGCCGATgggg                                                                                                                      >  2:1707341/1‑239 (MQ=255)
                                                                                                                   ttttagaggtgcaaaaaaaGAAAAACCCAC‑ATTTTTA‑GTTGTTGTTGGGGGCGG‑TA‑AATTTTCCCAGA‑AAAATAC‑AAAAAATGAA‑TTTGCATTGATGAGA‑GAAATTAATAAGCAAC‑AGGCGGTGAATAAGTTGACAGAG‑ATTGTCGT‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                     <  2:1406998/226‑1 (MQ=255)
                                                                                                                   tatgcgcGGTGGCAAAAAATAAAAAACCAA‑ATTGTTA‑GTGGTAGGTTGGGGGGG‑TG‑AATTTTCCAAGA‑CAAAAAC‑AAAAACTGGG‑GTGGCAATGAGGAGC‑AAAAAAAATAAGCAAC‑AGGCAGTGAATAATTGAACAGAG‑ATTGTGGT‑AAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                     <  1:77868/233‑1 (MQ=255)
                                                                                                                   tatTCACGGTGGAAAAAAATAAAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAAA‑AAAATAA‑AAAAAGTGGA‑GTGGCCATGATGAGC‑AAAATGAAAAAGCAAC‑AGGCAGTGAATAAGTTGAAAGGT‑ATTGGCGC‑TAATTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                     <  2:1589960/239‑1 (MQ=255)
                                                                                                                   atgtctgtggggaaaaaaaaaaaaaACCCC‑GTTTGTG‑GTGGTAGGTTGGGGCGG‑TG‑AATTTTTCCAGA‑CAAATAA‑AAAAACTGGA‑GTGCCCAGCAAGGGA‑AAAAGAAAGAAGCAAC‑AGGCAGTGAAAAAGTTGAGAGAG‑ATTGCCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                     <  1:499646/226‑1 (MQ=255)
                                                                                                                    atTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCTGA‑CAAAAACAAAAAACTGG‑‑GTTGGCCTGATGTGC‑GAAAGTAAAAAACAAC‑AAGCAAGGAATAAATTGACAGAG‑ATTGTCGC‑TAAATTTAACGCCATGAATTTTTCCCGAATGCCTGTTGACGTGGTGGGTAAAATAAAACAGCTAAAGGATGCCCGAACGTCGTCGATggggga                                                                                                                    >  2:1625485/1‑237 (MQ=255)
                                                                                                                    atTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAGA‑AAAAAAA‑AAAAAATGGA‑GGTGTCATGTTGAGC‑GAAAAGAATAAAAAAA‑AGGCAAGGAAAAAGTTGACAGAG‑AGTTTTGC‑TAAATTTTACGCCCTGATTTCTACCCGAATGCCTGTTAACGTGGGGGATAAAATAAAAAAGCAAAAAGAAAACCAAAACACGTCGATGGGGaa                                                                                                                    >  1:1027860/1‑239 (MQ=255)
                                                                                                                      tCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCCGA‑CAAATAC‑AAAAAATTGA‑GTTTCCCTGATGAGC‑GGAAAAAATAAACAAC‑AAGCAGTGAATAAGTTGACAGAG‑ATTGTTGC‑TCACCTTACCGCCATGATTTTTTCCCCAATGCCTGATGAAGTGGGGGGTAAACTAAAACAGCTAAAAGAAGCCCAAACATCGTCGATGGGGgaaa                                                                                                                  >  2:1420966/1‑239 (MQ=255)
                                                                                                                        aCGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTCAGA‑CAAAAAA‑AAAAAAATGA‑GTTGCCCTGATGAGC‑GAAAGTAATAAACAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTTCCCGAATGCCTGATGACGTGGTGGATAAACTAAAAAAACTAAAgg                                                                                                                                             >  2:965403/1‑210 (MQ=255)
                                                                                                                          ggTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑AAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                              <  2:164378/239‑1 (MQ=255)
                                                                                                                           ggggCAAAAAAGAAAAAACCAT‑ATGTTTG‑GGGGTAGTTTGTGGGGG‑TG‑ATTTTTGCAAGA‑AAAATAC‑AAAAACTGGT‑GTTGCAATGATGAGC‑AAAAGTAATAAGCAAC‑GTGGAGTTAATAAGTGGACAGAG‑ATTGCCGT‑TACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGAAAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                              <  1:1063215/236‑1 (MQ=255)
                                                                                                                           gTGGAAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACGGGA‑GTGGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                              <  2:838519/238‑1 (MQ=255)
                                                                                                                           gTGGAAAAAAAAATAAAACCA‑‑ATTTTTAGGTGGTAGTTGGGGGCGG‑TA‑AATTTTTCCAGA‑CAAATAC‑AAAAATTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAACAAC‑AGGCATTAAATAATTTAAAAGAT‑TTTGTCGC‑TATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                              <  1:458088/238‑1 (MQ=255)
                                                                                                                            tgtaaaaaaaTAAAAACCTAA‑ATTTTTA‑GTGGTGTTTTGTGGGGG‑AA‑AATTTTCCAAAA‑AAAATAC‑AAAAACGAGA‑GTGCCTATGA‑GAGCAAAAAGTAATAAGCAAA‑AGACAGAGAATAAGTTGACGGAG‑ATTGTCGC‑AAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACGAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATc                                                                                                             <  1:1313660/234‑1 (MQ=255)
                                                                                                                               aaaaaaaTATAAAACCAC‑ATTTTTA‑GTGGTGGTTTGTGGGGG‑TG‑AATTTTTCCAGA‑AAAATAC‑AAAAACGGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGT‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTAc                                                                                                          <  2:693594/237‑1 (MQ=255)
                                                                                                                                    aaaataAAACCTC‑ATTTTAC‑TTGTAAGTTTGGGGCGG‑TG‑ATTTTTTCAAAA‑AAAAAAA‑AAAACCTGGA‑GTTGACTTAAGTGGA‑GAACATAATAAACACC‑AGGAAGTGAATAAGTTGAAAGAA‑TTTGTCCT‑TAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                                    <  2:519224/236‑1 (MQ=255)
                                                                                                                                           aaCCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTTTAAA‑CAAAAAA‑AAAAAATGGA‑GTTTGCATTATGGGG‑GGAAGAAATAAAAAAC‑AAACAGAGAAAAAATTTACAAAG‑ATTGTCGC‑TAAACTTACCCCCACGATTTTTTCCCCAAAGCCCCGTGACGGGGGGGGTAAAACAAAAAAACCAAAAGATGTCCAAACATCGCGGGTGGGGGAAAATATCTAACaacagcagttcg                                                                                             >  1:139719/1‑228 (MQ=255)
                                                                                                                                            cccAC‑ATTTTTA‑GTGGTATTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGa                                                                                            <  2:1609537/238‑1 (MQ=255)
                                                                                                                                             ccAA‑ATTTGTA‑GTGGTTGTTTGTGGCGG‑TG‑AATTTTTACAGA‑CAAAAAA‑AAAAACGGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGac                                                                                           <  1:1185310/239‑1 (MQ=255)
                                                                                                                                               ac‑aTATTGA‑GTGGTAGGTGAGGGGGG‑TG‑AATTTTTAAAGA‑AAAAAAA‑AAAAACGGGT‑GTTCCCATGATGAGC‑GAAATAAATAAGAAAG‑AGCCAGTGAAAAAGTTGAAAGAG‑ATTGTGGG‑AAACTTTCCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGacaa                                                                                         <  1:792084/239‑1 (MQ=255)
                                                                                                                                                      ttA‑GTGGTAGTTTGGGGCGG‑TA‑AATTTTTCCAGA‑CAAATAA‑AAAAACTGGA‑GTTGCCATGATGAGC‑AAAAGTAAAAAGCAAC‑GGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCa                                                                                   <  2:850490/239‑1 (MQ=255)
                                                                                                                                                             gTAGTTTGTGGCGG‑TG‑AATTTTTTCCGA‑CAAATAC‑AAAAACTTGA‑GTTGGCCTGATGAGC‑GGAAGTAATAAACAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTTCCGCCCTGATTTTTACCCCAAAGCCTGATGACGTGGGGGGTAAACCAAAACAGCTAAAAGATGCCGCAACCTCGTCGATGGGGGAAAATATATACCATACGATTTTTGACAAAATGCaaaaaac                                                                             >  2:732356/1‑237 (MQ=255)
                                                                                                                                                               attttagggCTG‑TA‑AATTTTAACAGA‑AAAATAC‑AAAATCGTGA‑GTTGCAGTGATGAGG‑AAAGGTAGTAATCAAC‑AGCAAGTGAATAAGTTGCCAGAT‑TTTGTAGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGGGGTGGATAAACTAAAACAGCTAAAGGATGCCTAACCGGCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                           <  2:1229731/232‑1 (MQ=255)
                                                                                                                                                               aGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAACCTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                           <  2:396519/239‑1 (MQ=255)
                                                                                                                                                               aGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACGGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                           <  2:308395/239‑1 (MQ=255)
                                                                                                                                                               aGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAA‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGAAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                           <  2:139719/239‑1 (MQ=255)
                                                                                                                                                                 agttgggggggggaaaTTTTCACAGA‑AAAAAAA‑AAAAAGTGGA‑GTTGCAAGGAGGAAG‑AAAAGAAAAAAGCAAA‑AGGCAGTAAAAAAGTGGAAAGAG‑ATGGTCGA‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                           <  2:540806/225‑1 (MQ=255)
                                                                                                                                                                 agttggggggggaaaaTTTTTCCAGA‑AAAAAAC‑AAAAACGGGA‑GTTGCGATGATGATG‑AAAAGTAAAAAGCAAC‑AGGAAGTGAAAAATTGGACAGAG‑TGTGTCGC‑AAACTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                           <  1:1644899/225‑1 (MQ=255)
                                                                                                                                                                  tggtgGCGT‑AG‑AATATTACCGGA‑AAAAAAA‑AAAACGTGGA‑GTTGCAATGATGAAA‑AAAAGTAAAAACAAAC‑AGGCAGTGAAGAAGTGAACAAAG‑TTTGTGCT‑TAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGAtt                                                                         <  1:642651/236‑1 (MQ=255)
                                                                                                                                                                    gtgGCTG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACTGGA‑GTTGCAAGGATGATC‑AAAAGAAATAAGAAAC‑AGGCATTAAAAAATTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAc                                                                      <  2:1236633/239‑1 (MQ=255)
                                                                                                                                                                      tgCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGa                                                                       >  2:195466/2‑236 (MQ=255)
                                                                                                                                                                                    ttaccaaaaaaaaac‑aaaaacTGGA‑GTGGCCATGATGAGG‑GAAAGAAATAAGCAAC‑AGGCAGTAAATAAGTTGACAGAG‑ATTGTCGC‑TACTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                         <  2:1007273/230‑1 (MQ=255)
                                                                                                                                                                                     ttacaaaaaaaaaaaaaaagCGGGA‑TTTGCGATGATGACC‑GAAAGAAAAAACAAAA‑AGGCAGTAAAAAAGTGGACAGAG‑ATGGTCGC‑AAATTTTCCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCGGAATCGTcctg                                                         <  2:1105139/224‑1 (MQ=255)
                                                                                                                                                                                           aaaaaTAA‑AAAAACGGGA‑GTTGCGAGTATGAAC‑AAAAGAAATAAGCAAC‑AGGCAGTAAATAAGTTGACAGAG‑TTTGCCGC‑TATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCa                                                  <  1:195466/237‑1 (MQ=255)
                                                                                                                                                                                                       aaCTGGA‑TTTGCAAGGATGGGC‑GAAAGAAAAAAGCAAC‑AGGCAGTAAATAAGTGGACAGAA‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                       <  2:417638/239‑1 (MQ=255)
                                                                                                                                                                                                        aCTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGga                                      >  2:1480569/1‑239 (MQ=255)
                                                                                                                                                                                                           ggA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAt                                   >  2:536291/1‑239 (MQ=255)
                                                                                                                                                                                                           ggA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAt                                   >  1:849426/1‑239 (MQ=255)
                                                                                                                                                                                                            gA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAACCTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACACCATGCAAAAAGAGATTGAACTGAATCGTCCTGCCTGTCAGGACACCTGGGCGAtt                                  >  1:842566/1‑239 (MQ=255)
                                                                                                                                                                                                                  gCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGtt                             >  1:1455343/1‑239 (MQ=255)
                                                                                                                                                                                                                            gagaaaaGTAAAAAGCAAA‑AGGCAGTAAATAAGTTGACAGAG‑ATTGCGGC‑AACCTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGtt                      <  1:1556180/233‑1 (MQ=255)
                                                                                                                                                                                                                             c‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGa                                                                                                   >  2:1076207/1‑158 (MQ=255)
                                                                                                                                                                                                                             c‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg                  >  2:738429/1‑239 (MQ=255)
                                                                                                                                                                                                                             c‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGACGC‑TCACTTTACCGCCATGATTTCTACCCGAATGCCTGATGAAGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGCTTGACCTGAATCGTCCTGCCGGTCAGGACAGCGGGGAGATTATGTACTTCGTTAAAg                  >  2:978475/1‑239 (MQ=255)
                                                                                                                                                                                                                               aaaaGTAATAAGCAAC‑AGGAAGTAAATAAGGTGACAGAG‑TTTGTCGC‑TAACTTTGCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTaaa                   <  2:68886/236‑1 (MQ=255)
                                                                                                                                                                                                                                     aataaGCAAC‑AGGCAGTAAATAAGTTAAAGGGG‑ATTGTCAC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTc           <  1:1480569/239‑1 (MQ=255)
                                                                                                                                                                                                                                     aaaaaGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTc           <  1:1069861/236‑1 (MQ=255)
                                                                                                                                                                                                                                        aaGAAAC‑AGAAAGTGAAGAAGTAAGAAGAG‑ATTGTCGA‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCg        <  1:1152676/239‑1 (MQ=255)
                                                                                                                                                                                                                                              c‑AGGCAGTGAATACGTTGACAGCG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGATACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGAAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTca   >  1:1679296/1‑237 (MQ=255)
                                                                                                                                                                                                                                              c‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                                    <  2:1543781/141‑1 (MQ=255)
                                                                                                                                                                                                                                              c‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                                    >  1:1543781/1‑141 (MQ=255)
                                                                                                                                                                                                                                              c‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTccc  >  2:190426/1‑239 (MQ=255)
                                                                                                                                                                                                                                              c‑AGGCAGTGAATAAGTTAACAGAG‑ATTGTCGC‑TAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                              <  1:1127176/131‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 
CGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTTGTGGCGG‑TG‑AATTTTTCCAGA‑CAAATAC‑AAAAACTGGA‑GTTGCCATGATGAGC‑GAAAGTAATAAGCAAC‑AGGCAGTGAATAAGTTGACAGAG‑ATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCC  >  NC_000913/3206255‑3206723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑