BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,927,443:1	+C	5.2%	coding (290/993 nt)	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,927,443	1	.	C	5.2%	637.4 / 27.8	154	coding (290/993 nt)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand): ref base . (76/70); new base C (6/2); total (82/72)
Fisher's exact test for biased strand distribution p-value = 2.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.39e-01

Only 100 of 154 total aligned reads displayed.

CTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACT  >  NC_000913/3927212‑3927674

ctcGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCtt                                                                                                                                                                                                                                   <  2:84820/239‑1 (MQ=255)

  cGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTc                                                                                                                                                                                                                                  >  1:86646/1‑238 (MQ=255)

  cGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCg                                                                                                                                                                                                                                 <  2:15278/239‑1 (MQ=255)

    tCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGc                                                                                                                                                                                                                                >  1:87720/1‑238 (MQ=255)

     cAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGcc                                                                                                                                                                                                                               <  2:1015019/238‑1 (MQ=255)

     cAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGcc                                                                                                                                                                                                                               <  1:1136560/238‑1 (MQ=255)

                 cGTCTGGGGCTGATCGAAGTCCATGCGCCGATGCTTAGCCTTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAGGCGGTTCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCg                                                                                                                                                                                                                 <  1:817562/239‑1 (MQ=255)

                 cGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCCGCGCTGAAgtcg                                                                                                                                                                                                                  >  2:480869/1‑236 (MQ=255)

                   tCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGGCC‑CCGTGAAGGCCg                                                                                                                                                                                                                 >  1:941431/1‑237 (MQ=255)

                   tCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCg                                                                                                                                                                                                                 >  1:697316/1‑237 (MQ=255)

                            gATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGGCTTTCTCCGt                                                                                                                                                                                                      >  1:397256/1‑239 (MQ=255)

                                gAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCGTGATGCCCAGTGCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCa                                                                                                                                                                                                  <  2:695915/239‑1 (MQ=255)

                                  gccaggccaaactcttcgctaaccgcagcttcggttgctttaattcccgcccagatcgcctctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTG‑TACAG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACt                                                                                                                                                                                                >  2:1050856‑M1/172‑239 (MQ=255)

                                  aGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                                 <  2:1109524/238‑1 (MQ=255)

                                  aGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                                 <  2:931176/238‑1 (MQ=255)

                                  aGTCCAGGCGCCGAGTCTGAGCCGGGTGGGGGATGGCACGCAGGATAACTTGGCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACGGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCCGCGCTGAAgtcgtgttgccctaaggtct                                                                                                                                                                                                  <  1:480869/238‑20 (MQ=255)

                                      ggccaaactcttcgctaaccgcagcttcggttgctttaattcccgcccagatcgcctctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTG‑TACAG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCgg                                                                                                                                                                                             <  2:376550‑M1/71‑1 (MQ=255)

                                      cAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGt                                                                                                                                                                                            <  1:1127232/239‑1 (MQ=255)

                                      cAGGCGCCGATTCTTAGCCGTGTGGCGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGt                                                                                                                                                                                            <  1:271932/239‑1 (MQ=255)

                                      cAGGCGCCGATTCTGAGCCGTGGGGGGGATGGCACGCAGGATACCTTGCCGGGTTGTAAAAAAGCGGTGCAGGTAAAAGTGAAACCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATCCACGGGCGAAGTGAAAACGTCAGACCTTAGGGCAACACGCCTTCAGCGCGGGCGAAGGGCTGTCCACGCACCTG‑AA‑AG‑CCTTTCGCCC‑CAATAAAGCCCGTCTTTCTCCGTTGCACCCGGt                                                                                                                                                                                            <  1:80508/239‑1 (MQ=255)

                                      cAGGCGCCGATGCTTAGCCGTGTGGGGTATGGCACGCAGGATAACGTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGt                                                                                                                                                                                            <  1:835952/239‑1 (MQ=255)

                                         gcgcCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTa                                                                                                                                                                                         >  1:1201662/1‑239 (MQ=255)

                                           aactcttcgctaaccgcagcttcggttgctttaattcccgcccagatcgcctctaccgtgctttccagagtcgagaattgacgctcaccgtcgcccattacgcgttcccaggcccactggtcaacatagaccgagtgcaacggagaaagacggtctctctcgGGTCGAAGGGCTTT‑CATGTGCGTG‑TA‑CGGCCCTTCGCCC‑CGATGAAGACCGTCTTGCTCCGTTGCACTCGGTCTATg                                                                                                                                                                                       >  1:897147‑M1/163‑239 (MQ=255)

                                            ccGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGGAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGCAGTGGAAACGTCAGACCTTAGGTCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AC‑AG‑CCCTTCGCCC‑CGCTGAAGACCGTCTTTCTCCGTTGCACTAGGCCTCTGt                                                                                                                                                                                      >  1:889627/1‑239 (MQ=255)

                                             cGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCT‑TTCATGTGCGTGTAC‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGtt                                                                                                                                                                                     <  2:357591/239‑1 (MQ=255)

                                              gATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                                 <  1:94217/226‑1 (MQ=255)

                                                  cgctaaccgcagcttcggttgctttaattcccgcccagatcgcctctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTG‑TACAG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCa                                                                                                                                                                                >  2:396370‑M1/156‑239 (MQ=255)

                                                  cTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCa                                                                                                                                                                                <  2:86646/239‑1 (MQ=255)

                                                    tAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGt                                                                                                                                                                              <  1:329779/239‑1 (MQ=255)

                                                        cGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGa                                                                                                                                                                          <  2:941431/239‑1 (MQ=255)

                                                          tgtgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                                 >  2:209742/1‑214 (MQ=255)

                                                          tgtgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTggg                                                                                                                                                                           >  1:1153624/1‑236 (MQ=255)

                                                            tgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGACGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTgg                                                                                                                                                                      >  2:463911/1‑239 (MQ=255)

                                                              ggggATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTggg                                                                                                                                                                     <  1:368755/238‑1 (MQ=255)

                                                               ttcggttgctttaattcccgcccagatcgcctctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTG‑TACAG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGaa                                                                                                                                                                   >  2:881‑M1/143‑239 (MQ=255)

                                                                  aTGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGGAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAAcgc                                                                                                                                                                >  2:435096/1‑239 (MQ=255)

                                                                         gtaatgcccgcccaaatcgcctctaccgatcgtttcagagtccagaaatgacgatcaccgaagcccatcacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTG‑TACAG‑CCCTGCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATgg                                                                                                                                                         >  1:861785‑M1/133‑239 (MQ=255)

                                                                               tAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTCCACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACg                                                                                                                                                   >  2:1223080/1‑239 (MQ=255)

                                                                                     gTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACCGTGAGc                                                                                                                                             >  1:66109/1‑239 (MQ=255)

                                                                                       cGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCg                                                                                                                                            >  2:1162133/1‑238 (MQ=255)

                                                                                             gtgAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACCCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAAt                                                                                                                                       >  1:54929/1‑237 (MQ=255)

                                                                                               ctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTG‑TACAG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTg                                                                                                                                                                                    <  2:878830‑M1/80‑1 (MQ=255)

                                                                                               ctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTG‑TACAG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTg                                                                                                                                                                                    >  1:878830‑M1/111‑190 (MQ=255)

                                                                                                   aaGCGGTGCAGGTAAAAGAGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGAct                                                                                                                               <  2:834195/239‑1 (MQ=255)

                                                                                                        gTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGACGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGCGGGTTCGCGTCAATTCGCGACTCTGaa                                                                                                                          >  1:763952/1‑239 (MQ=255)

                                                                                                        gTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATtct                                                                                                                                    >  1:485312/1‑229 (MQ=255)

                                                                                                        gTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATtct                                                                                                                                    <  2:485312/229‑1 (MQ=255)

                                                                                                        gTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGCCTCTGaa                                                                                                                          >  2:742752/1‑239 (MQ=255)

                                                                                                          gCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGaaaa                                                                                                                        >  2:1104071/1‑239 (MQ=255)

                                                                                                            gtgTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAg                                                                                                                       <  2:1018317/236‑1 (MQ=255)

                                                                                                            aGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGTAACCCGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCG‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAg                                                                                                                       <  1:134377/238‑1 (MQ=255)

                                                                                                            aGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAg                                                                                                                       <  2:536298/238‑1 (MQ=255)

                                                                                                               tAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCa                                                                                                                     <  2:1153624/237‑1 (MQ=255)

                                                                                                                aaacgggAAAGCTCTGCCTGATGTCCAGTTCGAAGTGGTTCATTGACTGCCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACGTCAGCGCTGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACgg                                                                                                                  <  1:152057/233‑1 (MQ=255)

                                                                                                                  aaGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTa                                                                                                                <  2:599574/239‑1 (MQ=255)

                                                                                                                   aGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCAACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTag                                                                                                               >  2:302987/1‑239 (MQ=255)

                                                                                                                        aaGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCg                                                                                                          >  2:657320/1‑239 (MQ=255)

                                                                                                                                             tCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTaaa                                                                                        <  1:1035495/236‑1 (MQ=255)

                                                                                                                                               gAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAAc                                                                                   <  2:675118/239‑1 (MQ=255)

                                                                                                                                                               tGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGc                                                                   >  2:633031/1‑239 (MQ=255)

                                                                                                                                                               tGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGc                                                                   >  2:106545/1‑239 (MQ=255)

                                                                                                                                                                   gAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGaa                                                                <  2:245590/238‑1 (MQ=255)

                                                                                                                                                                        ggAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGTCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGtt                                                           <  1:435096/238‑1 (MQ=255)

                                                                                                                                                                        ggAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGtt                                                           <  1:1075721/238‑1 (MQ=255)

                                                                                                                                                                                 aGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGc                                                  >  2:201681/1‑238 (MQ=255)

                                                                                                                                                                                 aGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACACGGTCTGTGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTGTCGACTCTGAAAAGCACGGTAGAGGCTATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGc                                                  >  1:962059/1‑238 (MQ=255)

                                                                                                                                                                                        aGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGtt                                            <  1:876056/237‑1 (MQ=255)

                                                                                                                                                                                        aGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGtt                                            >  2:876056/1‑237 (MQ=255)

                                                                                                                                                                                            cAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGc                                       <  2:833056/238‑1 (MQ=255)

                                                                                                                                                                                            cAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGc                                       <  1:181175/238‑1 (MQ=255)

                                                                                                                                                                                             aaCACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCg                                     >  2:1121896/1‑239 (MQ=255)

                                                                                                                                                                                               cacGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGa                                   >  1:275264/1‑239 (MQ=255)

                                                                                                                                                                                                acGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCAACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGAt                                  <  1:302987/239‑1 (MQ=255)

                                                                                                                                                                                                  gACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCa                                <  2:697316/239‑1 (MQ=255)

                                                                                                                                                                                                    tcttcatcgGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGa                              <  1:462654‑M1/230‑1 (MQ=255)

                                                                                                                                                                                                    tcttcatcgGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGAAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGa                              <  2:186300‑M1/230‑1 (MQ=255)

                                                                                                                                                                                                       ccagcgGGGCGAGGGGCGGTCCCCGAACATG‑AA‑CG‑CCCTTCGCCC‑CGATGAGGCCGGTCTTTCTCGGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGAAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCACCCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATcc                           >  1:396370‑M1/7‑239 (MQ=255)

                                                                                                                                                                                                       cAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATcc                           <  2:1121666/239‑1 (MQ=255)

                                                                                                                                                                                                           gcgGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACt                        <  2:59310/238‑1 (MQ=255)

                                                                                                                                                                                                              GGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGt                    <  1:1066400‑M1/239‑1 (MQ=255)

                                                                                                                                                                                                                  AAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTaca                 >  1:1112793‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                       CTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGcc            >  2:944447‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                       CTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGcc            >  1:800738‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                        TGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCa           >  2:405231‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                        TGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGCTTACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGACAAGCACGGTCGAGGCGATCTGGGCGGGAATTACAGCAACCGAATCTGCGGTTAGCGAAGAGTTTAGCCTTGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAg          <  1:741512‑M1/239‑1 (MQ=255)

                                                                                                                                                                                                                         gTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAgg         >  2:629654/1‑239 (MQ=255)

                                                                                                                                                                                                                          tACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGCGAGCGTCAATTCTCGACTCTGAACAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGACGCTGCGGTTAGCGGAGGGTGTGGCCTGGCACCGTTCCTGCCGGACAAGAGCAACGTCGTACACAGCCAGGa        >  1:282910/1‑239 (MQ=255)

                                                                                                                                                                                                                               gCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGtt     >  2:507206/1‑237 (MQ=255)

                                                                                                                                                                                                                               gCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGtt     >  1:575696/1‑237 (MQ=255)

                                                                                                                                                                                                                                 acaTG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGa                                                                 <  2:744126/175‑1 (MQ=255)

                                                                                                                                                                                                                                 acaTG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGa                                                                 >  1:744126/1‑175 (MQ=255)

                                                                                                                                                                                                                                 acaTG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGtt     >  1:857210/1‑235 (MQ=255)

                                                                                                                                                                                                                                 acaTG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACt  <  2:54929/238‑1 (MQ=255)

                                                                                                                                                                                                                                    tG‑AG‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGCGGGACTGGGAACGCGTAATGGGCGCCGGTGTTCGTCCATTCTCTTCGCTGCAAAGCACGGTATAGCCGATCTGGGCGGGAATTAAATCCACCGAAGTTTCGGTTAGCGCAGGGTTAGGCCTGGCCCCGTTCCTCCCGGCTCAGCTCCACTTCTTACACATCCAgctgtt     >  1:260660/1‑227 (MQ=255)

                                                                                                                                                                                                                                    tG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGCCGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGtt     >  1:764352/1‑232 (MQ=255)

                                                                                                                                                                                                                                    tG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAACAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTac                  >  1:588988/1‑219 (MQ=255)

                                                                                                                                                                                                                                    tG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTACAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCCGATCCACTTCGTACACAGCCAGGCGtt     >  1:1055344/1‑232 (MQ=255)

                                                                                                                                                                                                                                    tG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGtt     >  2:840092/1‑232 (MQ=255)

                                                                                                                                                                                                                                    tG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGtt     >  1:816582/1‑232 (MQ=255)

                                                                                                                                                                                                                                    tG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGGAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGATCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGtt     >  2:747484/1‑232 (MQ=255)

CTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACT  >  NC_000913/3927212‑3927674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`