Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	766,690	T→A	14.9%	L236H (CTC→CAC)	mngA →	fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzymes: IIA component/IIB component/IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	766,690	0	T	A	14.9%	98.3 / 2.3	55	L236H (CTC→CAC)	mngA	fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzymes: IIA component/IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base T (32/12);  new base A (4/4);  total (39/16)
Fisher's exact test for biased strand distribution p-value = 2.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAA‑CGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTC‑AATAGTGCAA‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATGGCATTCCCG‑CGCTTTCAGTGCCTGT‑TGCCGAGCCGATTCGCCATGCAGAAG‑CGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAAT  >  NC_000913/766454‑766924                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    ggCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAA‑CGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTC‑a                                                                                                                                                                                                                                                  >  2:300617/1‑239 (MQ=255) ggCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAA‑CGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTC‑a                                                                                                                                                                                                                                                  >  2:13414/1‑239 (MQ=255)   cgacgaCGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAA‑CGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTC‑aa                                                                                                                                                                                                                                                 <  1:703542/238‑1 (MQ=255)   cgacgaCGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAA‑CGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTC‑aa                                                                                                                                                                                                                                                 <  1:987087/238‑1 (MQ=255)              gatgaGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAA‑CGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTC‑AATAATGCAA‑ACg                                                                                                                                                                                                                                     >  2:760445/1‑238 (MQ=255)                                       acaggtgggccaaccccccTTCTGCCTTTTTTCCCAC‑CGCGCAATCTTGCGTCGGCGACAGGCCCTGCGCGGGGGTTGTGCCCCACTCTTATAGGGCGGCGAAATATGGAGAAAAGGCGGGACGAAAACTGGGCGTAAA‑TTTTTACTTAAAAAAACAAGCCCCTACGGGTTTTGAGGGGGTTTAAACGGCGGAC‑C‑AACAA‑AAGAGTGAAA‑CCGCCTGTATTTTTGCGGCTGAA‑GTCg                                                                                                                                                                                                            <  1:352192/220‑1 (MQ=255)                                             gAGGCACGCAACCTTCTGCCTCTTTTTCCAA‑CGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAA‑C‑AAATC‑AAAAAAGCAA‑CCGCCTGGATTTTTTCGGGTGGA‑GTTGCCaaccaa                                                                                                                                                                                                    >  2:1126602/1‑234 (MQ=255)                                               cgcctcccaccgttctccTCTTTTCCCAACCCCCCCAAGTTTCGCCTGCGTAACGGCCTGCCGGGGGGTTTTTCCCCACACCTATATGGCGTGGAAATATCGGAAAAAAGCCGGACGCAAAAGGGGAGAAAT‑TTTTTACGTTAAAAAAAAAGGCGCCAGCGGAATTGAGGGGGTTTAAACGGGGGAC‑C‑ACATA‑AATAGTGCAC‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAg                                                                                                                                                                                                   <  1:1126602/222‑1 (MQ=255)                                                      aaCCTTCTGCCTCTTTTTCCAA‑CGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑G‑CAAAC‑AATAGAGCCA‑ACCCCCGTCTTTTTTCTGCTGAG‑Gttccgcccaatgggcggagg                                                                                                                                                                                           >  1:736650/1‑220 (MQ=255)                                                                  ctTTTTCCAA‑CGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTC‑AATAGGGCAA‑ACGCCTGTATTTTTGCGGCTGGA‑AGCGCCA‑TCAAGGAGAGTGAG‑G‑GGTTTTATGGc                                                                                                                                                                            >  2:812423/1‑239 (MQ=255)                                                                        ccAA‑CGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAA‑C‑AACTC‑AATAGTGCAA‑CCGCCTGTATTTTTGCGGCTGGA‑GTCGCCA‑TCAAGGGGAGGGGG‑C‑GGTTTTATGGCAtttc                                                                                                                                                                       >  2:551514/1‑236 (MQ=255)                                                                              gcgcCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTC‑AATAATGCAA‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCC‑TCAAGGAGAGGGGG‑C‑GTTTTAATGGCATTCCCG‑CGCt                                                                                                                                                                >  2:1213775/1‑239 (MQ=255)                                                                                                  aaCGGCCTGTCCGGCGGGGATTGCTCACACCTATATGGGTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑G‑AAACT‑ACAAATGCGA‑ACCCCCGGATTTTTTCGGCTGGA‑GTCGCCACTCATGG‑GAGTGAGAC‑GTTTTAAT‑GCATTACCG‑CGCTGTTAGTGCCCGT‑Ttcccag                                                                                                                                            >  1:78121/1‑238 (MQ=255)                                                                                                         ggcccGGCGGTTTTTGCCCACCCCAATAGGGCTGCGAAATATAAGAAAAAGGCCGGACGAAACCTGGGCGAAAT‑TTTTTAC‑TTAAAAAAACAGGGGCTAACGGGAATAAAGGGGCTTTAACGGGGGAA‑CAAACTA‑AATGGTCAAA‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATGGCATTCCCG‑CGCTTTCAGTGCCTGT‑TGCCGAGCCGATTCg                                                                                                                                    <  2:291866/236‑1 (MQ=255)                                                                                                           tCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑T‑CAAGC‑CATACTACAA‑ACCCCTGTATTTTTTTGGCCTGA‑GTCGCCA‑TCAAGGGGAGGGAG‑CGGTTTTATTGAATTTCCCG‑CG‑TTTTAGTGCCTGT‑TGGCGAGGCGATTTGc                                                                                                                                   >  1:370240/1‑238 (MQ=255)                                                                                                                 ggttATTGCTAACACTAATAGGGCGGGGGAATATCTGAAAAAACCGGGACGAAAACTCGGTGAAAA‑TTTTTACGTAAAAAAACAAGGCGCTAACGGATTTGAGGGGCTTTTAAGGGCGGAA‑C‑AACTC‑AATAGTGAAA‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATGGCATTCCCG‑CGCTTTCAGTGCCTGT‑TGCCGAGCCGATTCGCCATGCa                                                                                                                             <  2:1144935/235‑1 (MQ=255)                                                                                                                     aTTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTC‑AATAGTGCAA‑CCGCCTGTATTTTTTCGGCTGGA‑GTCGCCA‑TCAAAGAGAGTGAG‑C‑GTTTTTATGGCATTCCCG‑CGCTTTCAGTGCCTGT‑TGCCGAGCCGATTCGCCATGCAGAAG‑c                                                                                                                       >  2:1179962/1‑239 (MQ=255)                                                                                                                     aTTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAAAAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGGA‑T‑AAAAC‑AATAGTGCAA‑AACCCTGTATTTTTTCGGCCGAAGGGTGCCT‑AACAGAGGGGGGGG‑G‑GTTTTAAT‑GCATTTCCG‑CGCCTTCAGAGCCCGT‑TGCCGCGCCGATTAGCCAttgcgaagc                                                                                                                        >  1:276644/1‑231 (MQ=255)                                                                                                                             cacCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTC‑AATAGTGCAA‑CCGCCTGTATTTTTTCGGCTGAA‑GTCGCCA‑TCAAGGGGAGAGAG‑C‑GGTTTAATGGCATTCCCG‑CGCCTTCAGTGGCTGT‑TGCCGCGCCGACTCGcccctcaggaacgttggtc                                                                                                                 >  1:188993/1‑221 (MQ=255)                                                                                                                              acCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTC‑AATAGTGAAA‑CCGCCTGTATTTTTGTGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATTGCATTTCCG‑CGCGTTCTGTGCCTGT‑TGCCGAGCCGATTTGCCATGGAGAAG‑GGTTgtcaca                                                                                                              >  1:1108417/1‑234 (MQ=255)                                                                                                                                 ttatgggcggggaaaTATCGGAAAAAAGCCGAACGAAAAC‑GGGGGTAAATTGTTAACGTTAAAAAACAGGCCGCAAAGGGCATTGAGGGGCTTTAAAGGGGGGAT‑C‑AACTA‑AATGGTGCAA‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATGGCATTCCCG‑CGCTTTCAGTGCCTGT‑TGCCGAGCCGATTCGCCATGCAGAAG‑CGTTGATCcaaca                                                                                                           <  2:401600/226‑1 (MQ=255)                                                                                                                                   tatGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTC‑AATAGTGCCA‑CCGCCTGTATTTTTGCGGCTGAA‑GGCGGCA‑TCCAGGGGAGGGAG‑C‑GTTTTAATGGCATTTCCG‑CGCTTTTCGTGCCTGT‑TTCCCGGCCGATTTGCCATTCAGCAA‑CCTTGATCcaacaa                                                                                                          >  1:456592/1‑238 (MQ=255)                                                                                                                                        ccgCGGCATATCTGGAAAAAGCCGGTCGCAAACTCGGCGTAAA‑TGCTTACGTTGAAACACTCGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑T‑AACTC‑CAAAATGCAA‑CCGCCTGCATTTTTTCGGCTGAA‑GTCGCCC‑TTAATGAGAGTGGG‑C‑GCTTTAATGGCTTTTCCG‑CTCCTTCAGTGCCTGT‑TGCCGAGCCGATTCGCCATGCAGAAG‑CGTTTATCCAACCAGCGAtt                                                                                                    >  1:557255/3‑239 (MQ=255)                                                                                                                                        cTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGCT‑C‑AACAC‑AATAGTGCAA‑CCGCCTGTATTTTTTCGGCTTCA‑GGCGCCA‑CCAAAGAGAGGGAG‑C‑GGTTTTTTTGCATTCCCG‑CTCTTTTAGTGCCTGT‑TGCCGAGACGATTCGCCATGCAGAAG‑CGGTGTTCCAACAAGAGCtt                                                                                                    >  1:525186/1‑239 (MQ=255)                                                                                                                                        cTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTC‑AATAGTGCAA‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATGGCATTCCCG‑CGCTTTCAGTGCCTGT‑TGCCGAGCCGATTCGCCATGCAGAAG‑CGGTGATCCAACAAGCGCtt                                                                                                    >  2:1188598/1‑239 (MQ=255)                                                                                                                                        cTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AAACC‑AATAGTGCAA‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGGGGGAG‑G‑GGTTTAATGGGATTCCCG‑CGCTTTCATTGCCTGT‑TGCCGAGCCGGTTCGCCATGCCGGAG‑CGGTGGTCCAACCAGCGCt                                                                                                     >  1:539590/1‑238 (MQ=255)                                                                                                                                        cTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGAGGAT‑C‑AACTC‑AATAGTGCAA‑CCGCCTGTATTTTTGTGGCTGGA‑GTCGCCA‑TCCAGGAGAGTGAG‑C‑GGTTTAATTGCATTCCCT‑CGCTTTCCGTGCCTGT‑TGCCGGGCCAATTCGCCATCTCGAAG‑CGTTGATTcacccacggct                                                                                                     >  1:952940/1‑229 (MQ=255)                                                                                                                                          gCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑T‑AACTC‑AATAGTGCAA‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATGGCATTTCCG‑CGCTTTCAGTGCCTCT‑TGTCGCGCCGATTCGCCATGCCGAAG‑CGTTGATCCAACAAACGCTTa                                                                                                   >  2:1059215/1‑238 (MQ=255)                                                                                                                                          gCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTC‑CATAGTGCAA‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTTATTGCATTCCCC‑CGCTTTCCGTGCCCGT‑TGCCGAGCCGGTTCGCCCTGCCGAAG‑CGGTGATCCAACCAGCGCCTa                                                                                                   >  1:619485/1‑238 (MQ=255)                                                                                                                                          gCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTC‑AATAGTGCAA‑CCGCCTGTATTTTTTCGGCTGAA‑GTCGCCA‑TCAAGGAGAGGGAG‑C‑GTTTTAATGGCATTTCCG‑CGCTTTCAGTGCCTGT‑TGCCGAGCCGCTTCGGCATGCAGAAA‑CGTTGTTCCAACAAGCGCTTa                                                                                                   >  1:373444/1‑238 (MQ=255)                                                                                                                                          gCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTC‑AATAGTGCAA‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GGTTTAATGGCATTCCCG‑CGCTTTCAGTGCCTGT‑TGCCGAGCCGCTTCGCCATGCAGAAA‑CGTTGATTCAACAAGCGCttt                                                                                                   >  2:685431/1‑237 (MQ=255)                                                                                                                                          gCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTC‑AATAATGCAA‑CCGCCCGTATTTTTTCGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATTGCATTCCCG‑CGCTTTCAGTGCCCTT‑TGCCGCGCCGATTCGCCCTGCCGAAG‑CGTTTGTCCAACCACCGCCTa                                                                                                   >  1:1071377/1‑238 (MQ=255)                                                                                                                                          gCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAA‑C‑AACAC‑AATAATGCAA‑ACGCCTGTATTTTTGCGGCTGAA‑GGCGCCA‑TCAAGGGGAGGGGG‑C‑GTTTTAATGGCGTTCCCG‑CGCTTTCAGGGCCTGG‑TGCCGGGCCCGTTTGCCCCGCCGAAG‑CAGTGGTCCAACCAACGCCTa                                                                                                   >  1:497443/1‑238 (MQ=255)                                                                                                                                           cGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGCT‑C‑AACTA‑CATAGTGGAC‑CCCGCCGGCGTTTTGCCGCTGAA‑GACACCC‑CCAAGGGGAGTGAG‑G‑GCTTTACAAGCATTTCCGCCGCTTTAGGTG‑CTGTCTGCCG‑GGCGATTCACCCAGCAAAAG‑CGtttgttcacaaaaccgttaa                                                                                                  >  1:366274/1‑220 (MQ=255)                                                                                                                                           cGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTC‑AATAGTGCAA‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATGGCATTCCCG‑CGCTTTCAGTGCCTGT‑Tg                                                                                                                                                 >  2:457505/1‑192 (MQ=255)                                                                                                                                            ggAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTC‑AATAGTGCAA‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATGGCATTCCCG‑CGCTTTCAGTGCCTGT‑TGCCGAGCCGATTCGCCATGCAGAAG‑CGTTGATCCAACAAGCGCTTAcc                                                                                                 >  2:719635/1‑238 (MQ=255)                                                                                                                                             gaaataacgaaaaaaGCCGGGAGCAAACTGGGGGGAAATTTTTTATGTGAAAAAACAGGCCGCAGAGGGAATGGAGGGGGGTTTAGGGGAGGAT‑A‑AATAA‑AAGAGTGAAA‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATGGCATTCCCG‑CGCTTTCAGTGCCTGT‑TGCCGAGCCGATTCGCCATGCAGAAG‑CGTTGATCCAACAAGCGCTTAcc                                                                                                 <  1:13414/228‑1 (MQ=255)                                                                                                                                                          aaGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTC‑AATAGTGCAA‑CCGCCTGTATTTTTTCGGCTGCA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATGGCATTCCCG‑CGCTTTCAGTGCCTGT‑TGCCGAGCCGATTCGCCATGCAGAAG‑CGTTTATCCAACAAGCGCTTACCCTCAAGAGTAGCGAt                                                                                  >  2:143975/1‑239 (MQ=255)                                                                                                                                                                gACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTC‑AATAGTGCAA‑CCGCCTGTATTTTTTTGGCTGAA‑GGCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATGGCATTTCCG‑CGCTTTCCGTGCCTGT‑TGCCGAGGCGGATCGCCATGCAGAAG‑CGTTGTTCCAACAAGCGCTTACCCTCAAGCGTACCGGTgtaga                                                                             >  1:374061/1‑234 (MQ=255)                                                                                                                                                                           ggCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTC‑AATAGTGCAA‑CCGCCTGTATTTTTGCGGCTGAA‑GGCGCCC‑TCAAGGGGAGTGAG‑C‑GGTTTAATGGGATTCCCC‑CGCTTTCTCTGCCTGT‑TGCCGAGCCGATTCGCCATGCCGAAGCCGTTG‑TCCCCCAAGCGCCTCCCCTCCCGCGTAGCGCTGCGACGcgtaccgccc                                                                  >  1:423576/1‑235 (MQ=255)                                                                                                                                                                             cGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTC‑AATAGTGCAA‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATGGCATTCCCG‑CGCTTTCAGTGCCTGT‑TGCCGAGCCGATTCGCCATGCAGAAG‑CGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGc                                                               >  2:675702/1‑239 (MQ=255)                                                                                                                                                                                                aaaaCAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTC‑AATAGTGCAA‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATGGCATTCCCG‑CGCCTTTAGTGCCTGT‑TGCCGAGCCGATTCGCCATGCAGAAG‑CGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGCTGAGACGCGGACCGTACAGCAAGATACGCAACCGGTGa                                             >  2:99575/1‑239 (MQ=255)                                                                                                                                                                                                       ggCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTT‑AATTGCGAAA‑CCGCCTGTCTTTTTGGCGACGAA‑GACGCCC‑TCAAGGAGGGTGAG‑C‑GTTTTTATTCTCTTCCCC‑CGCTTCTACCGCCTGT‑TTCCGAGCCTGTTCGCCTTGGAGACC‑GGTTTATCAATCAAGGGACTACCCTCACGCCTAGCGATGCGAAGCGTTCCGTACCCAAAGATaggaacaccgcgcaacgtga                                      >  1:581239/1‑220 (MQ=255)                                                                                                                                                                                                            aatgggttgtGAAGGGGTTTAAACGGCGATA‑C‑AACTA‑AATAG‑GCAAGCCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATGGCATTCCCG‑CGCTTTCAGTGCCTGT‑TGCCGAGCCGATTCGCCATGCAGAAG‑CGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCaaaa                                 <  2:1095038/230‑1 (MQ=255)                                                                                                                                                                                                              cgggaaTTGAGGGGCGTTTAAGGGCGGAT‑C‑GACAA‑AATAGTGCAA‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATGGCATTCCCG‑CGCTTTCAGTGCCTGT‑TGCCGAGCCGATTCGCCATGCAGAAG‑CGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAAc                                <  2:430342/233‑1 (MQ=255)                                                                                                                                                                                                                  cATTGAAGGGCGTTTAACGGCGGAT‑A‑AACTC‑AAGAGTGCAA‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATGGCATTCCCG‑CGCTTTCAGTGCCTGT‑TGCCGAGCCGATTCGCCATGCAGAAG‑CGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAg                            <  2:736650/238‑1 (MQ=255)                                                                                                                                                                                                                          ggttttaagcgggggAT‑C‑ACCTA‑AATGGTGCAG‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATGGCATTCCCG‑CGCTTTCAGTGCCTGT‑TGCCGAGCCGATTCGCCATGCAGAAG‑CGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAAc                     <  2:497443/228‑1 (MQ=255)                                                                                                                                                                                                                            cGTTTAACGGCGGAT‑C‑AACTC‑AATAGTGCCA‑CCGCCTGTATTTTTTCGGCTGCA‑GTCGCCC‑TCAAGGAGAGTGAG‑C‑GTTTTAATGGCATTCCCG‑CGCTTTCCGTGCCTTT‑TGCCGAGCCGATTCGCCATGCAGAAG‑CGTTGATCCAACAAGCGCTTACCCTCAACCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAAACCGTGaa                                            >  2:785735/1‑212 (MQ=255)                                                                                                                                                                                                                            cGTTTAACGGCGGAT‑C‑AACTC‑AATAGGGCAA‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGGGTGAG‑C‑GTTTTAATGGCATTCCCG‑CGCTTTCAGTGCCTGT‑TGCCGAGCCGATTTGCCATGCAGAAG‑CGTTGATCCAACAAGCGCTTTCCCTCACGCGTCGCGCTGAGACGCGTACCCTACACCACAATACGCCACCGGGGAAAAACGGCAAAACGGAGCGGAAACACGc                 >  1:542571/1‑239 (MQ=255)                                                                                                                                                                                                                             ctttAACGGGGGATCC‑AACTA‑AATAGTGAAA‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATGGCATTCCCG‑CGCTTTCAGTGCCTGT‑TGCCGAGCCGATTCGCCATGCAGAAG‑CGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAgg                  <  2:1164672/237‑1 (MQ=255)                                                                                                                                                                                                                             ctgtAACGGGGGGA‑C‑AACACAAATAGGGAAC‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATGGCATTCCCG‑CGCTTTCAGTGCCTGT‑TGCCGAGCCGATTCGCCATGCAGAAG‑CGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAgg                  <  1:480008/235‑1 (MQ=255)                                                                                                                                                                                                                                   cggcggAT‑C‑AACTC‑AATAGTGCAA‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATGGCATTCCCG‑CGCTTTCAGTGCCTGT‑TGCCGAGCCGATTCGCCATGCAGAAG‑CGTTGATCCCACAAGCGCTTACCCTCAAGCGTAGCGCTGAGACGCGTACCGTACAGCAAGATACGCAAACGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTg          >  2:534735/1‑239 (MQ=255)                                                                                                                                                                                                                                      cggAT‑C‑AACTC‑AATAGTGCAA‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATGGCATTCCCG‑CGCTTTCAGTGCCTGT‑TGCCGAGCCGATTCGCCATGCAGAAG‑CGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGc       >  2:348181/1‑239 (MQ=255)                                                                                                                                                                                                                                            c‑AACTC‑AATGTGGCAG‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATGGCATTCCCG‑CGCTTTCAGTGCCTGT‑TGCCGAGCCGATTCGCCATGCAGAAG‑CGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAAt  <  2:188993/239‑1 (MQ=255)                                                                                                                                                                                                                                            c‑AACTA‑AATAGTGCAA‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATGGCATTCCCG‑CGCTTTCAGTGCCTGT‑TGCCGAGCCGATTCGCCATGCAGAAG‑CGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAAt  <  2:1071377/239‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    GGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAA‑CGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAA‑TGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAT‑C‑AACTC‑AATAGTGCAA‑CCGCCTGTATTTTTGCGGCTGAA‑GTCGCCA‑TCAAGGAGAGTGAG‑C‑GTTTTAATGGCATTCCCG‑CGCTTTCAGTGCCTGT‑TGCCGAGCCGATTCGCCATGCAGAAG‑CGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAAT  >  NC_000913/766454‑766924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑