Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 989,901 G→T 21.2% S152R (AGC→AGA)  pncB ← nicotinate phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913989,9010GT21.2% 143.4 / 9.1 90S152R (AGC→AGA) pncBnicotinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (24/45);  new base T (17/3);  total (42/48)
Fisher's exact test for biased strand distribution p-value = 8.45e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-01

GGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGC  >  NC_000913/989665‑990138
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 
gggCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑t                                                                                                                                                                                                                                                <  1:397332/239‑1 (MQ=255)
gggCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑t                                                                                                                                                                                                                                                <  2:166196/239‑1 (MQ=255)
      aTTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGGTT‑AAGGCGGAAAGGTGGAC‑AA‑TTTGC‑TTTTCA‑g                                                                                                                                                                                                                                         >  2:139380/1‑239 (MQ=255)
      aTTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AAAGACAAGAACTGGACTCA‑TTTAC‑TTTccc                                                                                                                                                                                                                                           >  1:91303/1‑237 (MQ=255)
      aTTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGGT‑AACGCCGAGGAGTCGCCTAA‑TTTGC‑TTTCCa                                                                                                                                                                                                                                           >  2:618671/1‑238 (MQ=255)
      aTTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGGTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGCAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGGT‑AAAGGCCAGAAGACGACCAA‑TATGC‑TCTCCa                                                                                                                                                                                                                                           >  1:975352/1‑238 (MQ=255)
       tttGGTGATGTGCCTGGAACCAGTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑Gc                                                                                                                                                                                                                                        <  1:331387/239‑1 (MQ=255)
             gATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCg                                                                                                                                                                                                                                  <  2:820805/239‑1 (MQ=255)
                gtgCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGCAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAGGCCCAAAAAAT‑‑AAGACCGGCGGGTT‑AAACCCGCGAAGTCGACTAC‑TTTGC‑TTTCAC‑CCGTGTCgg‑gg                                                                                                                                                                                                                              >  2:373052/1‑239 (MQ=255)
                gtgCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGGT‑AAAGCCGAAGAGGCGAACAA‑TTTTC‑TTTTCA‑GCggggcgggg                                                                                                                                                                                                                               >  2:1223854/1‑231 (MQ=255)
                gtgCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGAACTATC‑AAGACCGGC‑GGTTAAACACCG‑GAAGGCGACTTA‑TTTTG‑CTTCCA‑GGGTGGTGA‑gg                                                                                                                                                                                                                              >  2:652504/1‑239 (MQ=255)
                   gcTGGACCCGTTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTGCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGc                                                                                                                                                                                                                            <  1:673824/237‑1 (MQ=255)
                     tGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑TAGCCCCAGAAGGCTGCTAA‑TATGC‑TTTcccccgtcgcggtggtttg                                                                                                                                                                                                                           >  1:292231/1‑222 (MQ=255)
                            gttCGGGTGCCGGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGACAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACg                                                                                                                                                                                                                  <  1:604956/238‑1 (MQ=255)
                            aTTCGTGTGCCTGGGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACg                                                                                                                                                                                                                  <  1:649394/239‑1 (MQ=255)
                             ttCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAACAACGGCGAC‑GGGCGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AAAGCCGAAAAAGCGACCAA‑ATTTA‑TTTACA‑ACCTGTCGA‑GGGCtgtgcccacg                                                                                                                                                                                                                  >  1:644759/1‑229 (MQ=255)
                             ttCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGAGGGAAGCGCGACATATC‑AAGACCGGC‑GGCT‑AAAGCCGAGACGGCGACTAG‑TTTGA‑TTTTCA‑CAGGGGCGA‑GGGGTTgcgccaag                                                                                                                                                                                                                  >  1:1080338/1‑234 (MQ=255)
                             ttCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATAAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGAT‑AAAACCGAGAAGACGTCTAA‑AATTC‑TTTCCA‑CAGTCTCGA‑gagggattgcaccg                                                                                                                                                                                                                  >  1:959685/1‑225 (MQ=255)
                                       tgtgTTCCCTGCGGCGTGGGGGAAATCCGACCGGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTAGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCgg                                                                                                                                                                                                       <  1:634334/239‑1 (MQ=255)
                                          gtTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTg                                                                                                                                                                                                     <  1:348975/238‑1 (MQ=255)
                                          gtTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTg                                                                                                                                                                                                     <  1:666187/238‑1 (MQ=255)
                                            tCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGag                                                                                                                                                                                                   <  1:116440/238‑1 (MQ=255)
                                                 tCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGagcgatag                                                                                                                                                                                             <  2:900339/239‑1 (MQ=255)
                                                   ggCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGagcgatagcg                                                                                                                                                                                           <  2:251466/239‑1 (MQ=255)
                                                     cGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGGT‑AAAGCCGAGAAAGCGGATAA‑ATTGC‑TTTCCA‑GCGGGTCGA‑GGGGTTGCGCAAAGTCGGCCTGCGGGGggcgagagcggt                                                                                                                                                                                         >  2:890454/1‑237 (MQ=255)
                                                      gTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATg                                                                                                                                                                                        <  1:444842/239‑1 (MQ=255)
                                                      gTGAGGGAAAGCCGACGCGCCAGATCGTAGTGGCTGGTGCCCACAAACCGGGATTCCTGTTGCAGACGCTTAACGATGGCTTCTTGTACCTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGAAACGTCGGCCTGCGGTGAGCGATAGCGATg                                                                                                                                                                                        <  1:420003/239‑1 (MQ=255)
                                                       tGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATg                                                                                                                                                                                        <  1:789900/238‑1 (MQ=255)
                                                               aGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATT‑CTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AAAGCCCGGAAGGAGAATAATTTTGT‑TTTCCA‑‑CGTGGCGG‑GGGGTTGTGCAAAGTCGGCCTGGGGGGAGCGTAAGCGGTGGACCC‑tttt                                                                                                                                                                              >  2:1118632/1‑237 (MQ=255)
                                                                    aCGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGCGAAGTCGAACAA‑TTTGC‑TTTCCA‑GCGGGTGGA‑GGGCGTGCGCAACGTCCGCCTGCGGGGGGGGCTAGCCGGGTGCCA‑TTTCCCttg                                                                                                                                                                         >  1:1025507/1‑237 (MQ=255)
                                                                     cgcgCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGAGGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGa                                                                                                                                                                         <  2:91303/238‑1 (MQ=255)
                                                                       cgcCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGGGAAGTCGGCTCA‑TTTTC‑TTTTCA‑GCGTGTCGG‑GGGGTTGCGGAAACGCGGGCTGCCGGGGGCGGCAGCGGTGTACCC‑TTTCTCTGATa                                                                                                                                                                       >  1:1126734/1‑238 (MQ=255)
                                                                            acgcGTAGTTGCGGGGCCCCACAAACCAGGATACCGTTGGCAGCCGTTTAACGATGGTTTTTTGTACTTCGCGAGAAAAACGGCGCC‑GGGGGCCAAAACCCATCAGATGGAAGCGCGACATACC‑AAGCCCGGC‑GGTT‑ACCGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGGCGA‑GGGCTTGCGCAACTCCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGcc                                                                                                                                                                 <  1:1036234/236‑1 (MQ=255)
                                                                                  aGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAACCGGCGAC‑GGGTGCCAAAACCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCaa                                                                                                                                                            <  2:952787/238‑1 (MQ=255)
                                                                                  aGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGGCGGCTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGGTTGCGCCACGTCGGCCCGCGGGGAGCGATAGCGATGTACCA‑TTTCACTGATAAACGCCCGCaaa                                                                                                                                                           >  2:1005922/1‑239 (MQ=255)
                                                                                         ggTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGGGAGCGGTAGCGATGTACCC‑TTTCACTGATAACCGCCCGCAAAGGAACt                                                                                                                                                     >  2:477396/1‑238 (MQ=255)
                                                                                         ggTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AAAGCCGAGAAGTCGACTAA‑TTTGC‑TTTTCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGGTAGCGATGTAACA‑TTTCACTGATAAACCCCCGCCAAGGaaat                                                                                                                                                     >  1:1083384/1‑236 (MQ=255)
                                                                                                              cTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCC‑GCGTGTCGG‑GGGCGTGCGCAACGGCGGCCTGCGGTGGGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCCGCAAAGGAACATCCCAGAGGGTGAATTCACGc                                                                                                                                >  2:1147389/1‑238 (MQ=255)
                                                                                                                gTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGGT‑AAAGCCGCGAAGTCGACTAA‑ATTGC‑TTTTCA‑GCGGGGCGG‑GGGCCTGCGCAAAGTCGGCCTTCGGTGTGCGGTAGCGGAGGACCA‑GTTTAATGATAAACCCCCGGCAAGGAACTTTCCCGGGGATGGATTTACGccc                                                                                                                              >  1:563338/1‑237 (MQ=255)
                                                                                                                  tgaaGACGGTTACGGAGGGTTTTTTGTACTGCGCGAAAAAACGGGCGAG‑GGGTGCAAAAATCCATCAGAGGGAAGCGCGACATACC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTCCCA‑GCGTGTGGA‑GGGCTTGCGCAACGTCGGCCTGGGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACg                                                                                                                            <  1:696236/235‑1 (MQ=255)
                                                                                                                   gCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTTCA‑GCGTGTCGG‑GGGGTTGCGCAACGACCGCCTGCCGGGGGCGGTAGCGCTGGAACA‑TTTCACTCATAACCGCCCGCAAAGGCACTTCCCCGCGGGTGACCTctcccccccg                                                                                                                           >  1:373855/1‑229 (MQ=255)
                                                                                                                      gACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCCGGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGGGTCGG‑GGGGTTGCGCCAAGGCGGCCCGCGGGGAGCGCAAGCGATGTAACC‑TTTCTCTGATAAACCCCAGCAAAGGGAATTCCCCGGGGATGACTTCACGCCCCGGGcc                                                                                                                        >  1:383357/1‑238 (MQ=255)
                                                                                                                      gACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCCAGAAGTCGACTAA‑TTTTC‑TTTCCA‑GCGCGTCGG‑GGGCTTGCGCCACGTCGGCCCGCGGGGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCCGCAAAGGAACTTCCCCGAGGATGACTTCACGCCACGGGcc                                                                                                                        >  2:590856/1‑238 (MQ=255)
                                                                                                                                       tCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AAAGCCGCGAAATCGCCTAA‑TTTTC‑TTTCCA‑GCGTGTCGG‑GGGCTTGCGCCACCGCGGCCTGCGGGGAGCGGTAGCGGTGTAACA‑TTTCACTGGTAACCCCCCCCCAAAGAACTTTCCCGAGGGTGAATTTTAGCCCCGGGCCCCTTTAAAGAatttaca                                                                                                       >  1:378376/1‑233 (MQ=255)
                                                                                                                                         tttgaCTGCGCGAAAAAAGCGGCTAC‑GGGTCCCAATATCCAACAGAGGGAAGCGCGACAAATA‑GAGACCGGC‑GGTT‑AACGCCGAGAGGTCGACTAA‑TTTGC‑TTCCCA‑GCGGGTCGA‑GGGTTTGCGCAATTTCGGCCTGCGGTGAGCGATGAAGTTGTACAA‑TTTCCCTGAAACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCt                                                                                                    <  1:800021/235‑1 (MQ=255)
                                                                                                                                               ttCGCGAGAAAAACGGCGAG‑GGGTGCCAAAATCCATCAGAGGGAAGTG‑GAAATACCAAAGACCCGG‑GGTT‑AGCGCAGGAAGGTCCA‑TTA‑TTTTT‑TTTCCACGGTTCTCGA‑GGGCTTGCGAAACTTCGGCTTGGGGGGGGGGATGGGAATGTACCA‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCAt                                                                                              <  1:418862/239‑1 (MQ=255)
                                                                                                                                               tgcgcgAGAAAACCGGGGGC‑GGGGGAAAAAACCAAACGAATGGAAGCGGGACATATC‑AGGCCGGGC‑GGTT‑ACCGCCGAGAAGCCGACTAA‑TTTGC‑TTTCCA‑GCGTGTGGA‑GGGTTTGCGCAACGTCGGCCGGGGGTGAGCGAGAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCAt                                                                                              <  1:259729/237‑1 (MQ=255)
                                                                                                                                                 ggcgAG‑AAAACGGCGGCGGGGGGCCAAAAGCCATCAGACGAAAGCGCGACATTTC‑AAGCCCGGC‑GGTA‑AACGCCGAGAAGCCGCTTAT‑TTTGC‑TTCCAA‑GCGTGTGGA‑GGCCTTGCGCAACGTCGGCGTGCGGTGAGCGATAGGGATGTACCT‑TTTCCCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTa                                                                                            <  1:606028/238‑1 (MQ=255)
                                                                                                                                                 cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGCGAAGTCGACTAA‑TTTTC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGGTAGCGATGTACCA‑TTTCACTGATAACCCCCCGCAAAGGAACTTCCCAGAGGGTGACTTCACGCCACGGGGCGCCTAAAAGGAAATCCAACTTGCCAttt                                                                                            >  2:1103764/1‑238 (MQ=255)
                                                                                                                                                 cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCCAGAAGTCGACTAA‑TTTTC‑TTTTCA‑GCCGGTCGA‑GGGCTTGCGCAACCACGGCCTGCGGGGAGCGATAGCGGTGTACCA‑TTTTACTTATAAACCCCCGCAAAAGAAATTCCCACAGGAAGACTTCACCCCACCGGCCGACTAAAAGAATATCCAACCTGTCATTa                                                                                            >  1:737493/1‑239 (MQ=255)
                                                                                                                                                 cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AAAGCCGGGAAGTCGAATAA‑TTTTG‑TTTTCA‑GCGGGTCGG‑GGGGTTGGGCAACGGCGGGCTGCGGGGAGCGGTAGCGAAGGAACA‑TTTTACTGGTAAACGCCAGGAAAAGAACTTTCCAACAGATGAATTCCCGCCCACGGCCCCCTAAAAGAattaccacattgaccatt                                                                                            >  1:493135/1‑223 (MQ=255)
                                                                                                                                                 cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AAAGCCGAGAAGTCGACTAA‑TTTGC‑TTTTCA‑GCGTGTCCA‑GGGCTTGGGAAACATCGGCCTGCGGTGAGAGCTAAGGCTGTTCCA‑TTTCACTTAATAACGCCACACAAGAAACATCCCCGCGGGTGACCTCAACCCCCGCGGCCCTTAAACGGAAATCCaacctcacctta                                                                                            >  1:742550/1‑228 (MQ=255)
                                                                                                                                                 cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AAAGCCCAGAAACCGAAGAA‑TTTTG‑TTTTCA‑ACGGGGCGG‑GGGCTTGCCGCACGTCGGCCCGCCGTGGGCGACAGGGATGGACCA‑ATTCACAGATCACAGCCCGCAAAGGAAATTCCCACAGGAGGAATTCACCCCCCCGGCCCGTTAAACGaaatactcagctggcatta                                                                                            >  1:1043360/1‑222 (MQ=255)
                                                                                                                                                 cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGGT‑AACGCCCCGCAATCCACCAA‑TTTTT‑GTTCCC‑GCGTGTCGG‑GGGCTTGCGCCACGTCGGCCTGCGGTGAGCGGTAACGCTGGACCC‑TTTCTCTGATAAACGCCAGCAAAAGAACATCCCCGAGGCGGACTTACCCCCACGGGCCGCCTTAAcacaaatccccccctccctca                                                                                            >  1:903308/1‑219 (MQ=255)
                                                                                                                                                 cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGGT‑AACACCGAAAAGTAGACCAA‑TTTGC‑TTTTCA‑ACGTGGTGA‑AGGCTTTCGCAAAGTCGGGCTGCGGTTCGGGGTAGCGCGGAACCA‑GCTCATCGATTACCGCCACCAAACGAAGTTCCCCGAGGATGACGTCACTCCACGGCACCCTTAAAAGAATATCCAGCTTGGCAtgt                                                                                            >  1:699412/1‑237 (MQ=255)
                                                                                                                                                 cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGCT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCCTGTCGA‑GGGGTTTCGCAAAGTCGGCCTGCGGGGGGCGCTAGCGATGTAACA‑TTTCACTGATAACCCCCAGCAAAGGAACTTCCCAGAGGATGACTTTACCCCCCGGGCCGCCTAAAAGAATATCCAGCTTGGCATTa                                                                                            >  1:125872/1‑239 (MQ=255)
                                                                                                                                                   cgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCCGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGTTAACCGCCCGCAAAGGAACTTCCCAGAGGGTGACTTCACCCCACGGGCCGCCTAAAAGAAAAATCAGCTTTCCATTATc                                                                                          >  2:105888/1‑239 (MQ=255)
                                                                                                                                                       aaaaaCGGCGGG‑GGGTCGCAAACCCCACAAGATGGAACGGCGACATACA‑AAAGCGGGC‑GGTT‑AACGCAGAGAAGTCG‑CTTA‑TTTGCTTTTCCA‑GCGTGTGGA‑GGATTTGGCCAAGGTCGGCCTGGGGTGGGCGAAAGGGATGTCCCA‑TTTCATGGAGAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTg                                                                                      <  1:136731/239‑1 (MQ=255)
                                                                                                                                                                                     agagGGAAGGGGGACATAAC‑AAGACGGGG‑GGTA‑ACCGCGGAGAAGCCGACTAT‑TTTGC‑TTCCAG‑GGGGGGCGG‑GGGCTGGCGAAACGTCGGCCGGCGGGGGGCGATGGCGATGTCCCA‑TTCCACTGATACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAg                                                           <  1:590856/237‑1 (MQ=255)
                                                                                                                                                                                         ggAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGGCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCGTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAAACACCCGCAAAAGAACTTCCCAAAGGGAGACTTCACGCCCCCGGGCGCCTAAAAGAAAATCCACCTCGCCATTATCGTTTGTCACGGTGACTTGTTTCGGGTTTAAGCGGaa                                                      >  1:79422/1‑238 (MQ=255)
                                                                                                                                                                                           aaGCGCGACATATC‑AAGACCGGC‑GGTT‑AAAGCCGAGAAGTCGACTAA‑TTTTC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAAAGTCGGCCCGCGGGGGGCGAAAGCGATGTACCA‑TTTCACTGATAACCGCCAACAAAGGAACTTCCCAGAGGGTGACTTCACGCCCCCGGCCGCTTCAACGAAAATCCCACTTTCCATTTTCGTTTGACACGGTGACTTGGTCCGGGGTAAAGCGGaaacc                                                   >  1:48225/1‑236 (MQ=255)
                                                                                                                                                                                                cgACATATC‑AAGACCGGC‑GGTT‑AAAGCCGAGAAGTCGACTAA‑TTTTC‑TTTTCC‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGGGAGCGATAGCGATGTACCA‑TTTTACTGATAACCGCCCGCAAAAGAAATTCCCAGAGGGTGACTTCCCGCCACGGGCCCCTTAAAAGAATATCCAGCTTTCCATTATCGTTGGACACGGTGACTTGTTCCGGGGTTAAGCGGAACTCGCGTa                                              >  2:822188/1‑239 (MQ=255)
                                                                                                                                                                                                 gccacatacaaagccggggtgTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGAAACGTCGGCCTGCGGTGAGCGATAGCGATGTCCCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTcgc                                                 <  1:620542/217‑1 (MQ=255)
                                                                                                                                                                                                 gacaagtC‑AAGACCGGG‑GGAT‑AACGCCGAGAAGTCGACGAT‑TTTGC‑TTTCCG‑GGGGGTCGA‑GGGCTTGCGCAACGCCGGCCTGCGGTGGGTGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                             <  1:671591/233‑1 (MQ=255)
                                                                                                                                                                                                 gACATTAC‑AAGCCGGGG‑GGTT‑ATCGCCGAGAGGTCGGCTAA‑TTGTC‑TTGCCG‑TCTGTTCGG‑GGGCTGGCAAAACGCCGCCGGGGGGGGGGCGATAGCGTGGTACCA‑TTCCTCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                             <  1:1118632/239‑1 (MQ=255)
                                                                                                                                                                                                 gACATATC‑AGGCCCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTGGCGCAACGTCGGCCTGCGGTGGGCGATAGCGATGTCCCT‑TTTCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                             <  2:48225/239‑1 (MQ=255)
                                                                                                                                                                                                  atatatA‑AAGACCGGC‑GCTC‑ACGGGCGAGAAGTCGCCTAA‑TTTGC‑TCTCCA‑GCGTTTCGA‑GGGCTCGCGCAAGGTGGGCCGGCGGTGAGCCAAGGCGATGTACTA‑TTTCACTGAGCGCCGCCAGCAAAGGAACTTCCCAGAGGATGACTGCGCGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                             <  1:608915/236‑1 (MQ=255)
                                                                                                                                                                                                   acagtaaaggcccGGG‑GTTT‑CACGCCGAGAAGCCACTTAT‑TTTTC‑TTCCCT‑GCGTGGCGT‑GGGTTTGCGAAACGGCGGCCTGGGGTGAGTGAATGCGTGTTACCA‑TTTAACTGAGACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                             <  1:128173/227‑1 (MQ=255)
                                                                                                                                                                                                   aaatacgaaGACGGGC‑GGTT‑AGCGCCGAGAAGTCGGCTAT‑TTTGC‑TTCCCG‑GCGTGGCGA‑GGTCTTGCGAAACGCCGGCCTGGGGTGAGCGATAGCGAGTTACCA‑TTTCACTAATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                             <  1:1147389/231‑1 (MQ=255)
                                                                                                                                                                                                    aatagcagacccGGA‑GTTT‑AACGCCGAAAAGTCGTCTAT‑TTTGC‑TTTCCG‑GCGTGTCGA‑GGGCTGGCGAAGCGCCGGCCGGGGGTGAGGGATAGGGATGTACCA‑TTCCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAAc                                            <  1:105888/228‑1 (MQ=255)
                                                                                                                                                                                                       tC‑AAGACCGGC‑GTTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCG‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTCCCA‑TTTCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGtt                                       <  2:506583/239‑1 (MQ=255)
                                                                                                                                                                                                        c‑AAGGCCGGC‑GGTA‑AACGCCGAGAAGCCGTCTAA‑TTTGT‑TTCCCA‑GGGTGTCGA‑GGGCTTGCGCAACCTCGGCGGGGGGGGGGCGAAAGCGATGGCCCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTa                                      <  1:101964/239‑1 (MQ=255)
                                                                                                                                                                                                               cggc‑ggTT‑AACGCCGAGAAGTCGACTAA‑TTTTC‑TTTCCA‑GAGTGGCGA‑GGGCTTGCGCAACGTCGGGCCGCGGGGAGCGCTAGCGATGGACCA‑TTTCACTGATAACCGCCCGCAAAGGAAATTCCCAGAGGATGACTTCACGCCACCGGCCGCTTAAACGAATATCCAGCTTGCCCTTATCGTTGGCCACGGGGACCTGTTCCGGGGTAAAACGGGACTCGCGGAAACAGTTAAGATaa                                >  1:770658/1‑238 (MQ=255)
                                                                                                                                                                                                                ggc‑ggTT‑CACGCCGAGAAGTCGACTAA‑TTTTC‑TTTCCA‑GCGTGTCGG‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCTCTGATAAACCCCCGCAAAGGAACTTCCCCGAGGATGACTTCACGCCCCGGGGCGCTTAAACGAATATCCAGCTTGCCATTATTGTTGGACCCGGTGACTTTTTCCGCGTTAAAGCGGCCCACGCTGAACCAGCTAAAATat                                >  1:865326/1‑237 (MQ=255)
                                                                                                                                                                                                                 gc‑ggTT‑AACCCCGAGAAATCGACTAA‑TTTTC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGGATCC‑TTTCACTGATAACCGCCAGCCAAGGAACTTCCCAGAGGGTGACTTCACGCCCCCGGCCGCCTAAACGAAAATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTc                              >  1:162345/1‑239 (MQ=255)
                                                                                                                                                                                                                 gc‑ggTG‑ACCGGCGAGAAGTGGATAAT‑TTTGC‑TTTCCG‑GGGTGTCGA‑GGGTTGGCGAACCGCCGGCCTGGGGAGGGCGATAGCGATGACCCA‑TTTCACGGATAGCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                               <  1:508199/238‑1 (MQ=255)
                                                                                                                                                                                                                  ggggTT‑ACCGCCGAGAAGCCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                               <  2:1083384/236‑1 (MQ=255)
                                                                                                                                                                                                                       t‑AACGCCGAGAAGTCGTCTAT‑TTTGC‑TTTCCG‑GCGTGTCGG‑GGGTTTGCGAAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCT‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                         <  2:383357/239‑1 (MQ=255)
                                                                                                                                                                                                                       t‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCG‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                         <  2:770658/239‑1 (MQ=255)
                                                                                                                                                                                                                       t‑AACGCCAAGAAGCCGACTAA‑TTTGC‑TTTCCG‑GCGTGTCGA‑GGCCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                         <  2:860154/239‑1 (MQ=255)
                                                                                                                                                                                                                        tagcgacGAGAAGTCTACTAA‑TTTGC‑TTTCCA‑GCGGTTCGA‑GGTCTTACGCACCGTCGGCCGGCGGTGAGGGATAGCGATGTACCA‑TTCCACTATTAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                         <  2:956524/233‑1 (MQ=255)
                                                                                                                                                                                                                        taaggcgaagagGTCGACTAA‑GTTGC‑TTTCCT‑GCGTGTCGG‑GGGCTGGCGCAGCGGCTGCCGGGGGTGAGCAATAGGGATG‑ACCATTTTCACTAAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                         <  1:373052/231‑1 (MQ=255)
                                                                                                                                                                                                                             ccGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTaaag                    >  1:261482/1‑239 (MQ=255)
                                                                                                                                                                                                                                     ccGACTAT‑TTTGC‑TTCCAA‑GCGTGTGGA‑GGGCTGCCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAata                                                                                                           <  1:1051242/143‑1 (MQ=255)
                                                                                                                                                                                                                                      cGACTAA‑TTTGC‑TTTCCA‑‑CGGGTCGACGTGCGGGGCAAACGTCGGCCGGCGGTGCGGCATAGCGTTGGCCCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAggca             <  1:553477/237‑1 (MQ=255)
                                                                                                                                                                                                                                            a‑TTTGC‑TTCCCG‑GGGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGGGATAGCGATGTACCA‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGaa     <  2:756915/239‑1 (MQ=255)
                                                                                                                                                                                                                                            a‑TTTGC‑TTCCCA‑GCGTGTCGG‑GGGCTTGCGCAACGTCGGCCTGCGGGGAGCGATAGCGATGTCCCA‑TTTCACTGATACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGaa     <  2:31666/239‑1 (MQ=255)
                                                                                                                                                                                                                                                tGC‑TTTCCG‑GCGTGTGGG‑GGGCTTGCGCAACGTCGGCCTGGGGTGGCAGATAGCGATGTCCCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGc  <  2:368407/239‑1 (MQ=255)
                                                                                                                                                                                                                                                tGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGc                                                                                                 >  2:1051242/1‑144 (MQ=255)
                                                                                                                                                                                                                                                tGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGACCTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAg   >  1:458893/1‑238 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 
GGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGC‑GGTT‑AACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGC  >  NC_000913/989665‑990138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑