Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	256,597	T→C	15.4%	V24A (GTC→GCC)	frsA →	fermentation‑respiration switch protein, PTS Enzyme IIA(Glc)‑binding protein, pNP‑butyrate esterase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	256,597	0	T	C	15.4%	250.4 / 5.1	91	V24A (GTC→GCC)	frsA	fermentation‑respiration switch protein, PTS Enzyme IIA(Glc)‑binding protein, pNP‑butyrate esterase activity
Reads supporting (aligned to +/- strand):  ref base T (63/10);  new base C (2/14);  total (66/25)
Fisher's exact test for biased strand distribution p-value = 2.36e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAAT‑CTCCGGTCGCTAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACCGACGATGATTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAG  >  NC_000913/256362‑256826                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                      tCCCGCAAGATCCCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATCCGTCCCGCCAAT‑CTCCGGTCGCTAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTccg                                                                                                                                                                                                                                    <  2:125950/239‑1 (MQ=255) tCCCACAGGAGACCTGGAGTGATCAGCAATGGGATGTGGTGTGCGGTTCCTCCCCGCCAAG‑CTCCGGTCGCTAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTG‑CC‑AGTAAGTATTCTGGAGGCTG‑CAACCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTGTAAACATCCTGAA‑A‑CCGCGACGCTAGTccg                                                                                                                                                                                                                                    <  1:887492/239‑1 (MQ=255) ccccGAAAGATCCCGGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAAT‑CTCCGGTCGCTAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTccg                                                                                                                                                                                                                                    <  1:949070/238‑1 (MQ=255)  cccGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAAT‑CTCCGGTCGCTAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTccgc                                                                                                                                                                                                                                   <  2:44563/239‑1 (MQ=255)   ccGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAAT‑CTCCGGTCGCTAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTccgcc                                                                                                                                                                                                                                  <  2:1086412/239‑1 (MQ=255)     gCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAAT‑CTCCGGTCGCTAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGTAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTccgcc                                                                                                                                                                                                                                  >  2:272689/1‑237 (MQ=255)     gCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAAT‑CTCCGGTCGCTAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTccgcc                                                                                                                                                                                                                                  >  1:339971/1‑237 (MQ=255)             ccTGTATTGAACAGCCGTGGGATATGGGCGTCGAATTCGTCCCGCCAAT‑CTCCGGTCGCTAATCTTTTCAACGCCTGT‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCa                                                                                                                                                                                                                        <  2:394533/239‑1 (MQ=255)              cTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAAT‑CTCCGGTCGCTAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCAc                                                                                                                                                                                                                       >  2:730521/1‑239 (MQ=255)              cTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAAT‑CTCCGGTCGCTAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCAc                                                                                                                                                                                                                       <  1:487626/239‑1 (MQ=255)                 gATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAAT‑CTCCGGTCGCTAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGgc                                                                                                                                                                                                                    >  2:652236/1‑239 (MQ=255)                     gaccAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAAT‑CTCCGGTCGCTAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAACCATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGcac                                                                                                                                                                                                                >  2:193735/4‑239 (MQ=255)                      aaCAGCCGTGGCATATGGGTGTCGTATTCGGCCCGCCACT‑CTCCGGTCGCTAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGCCGATAGTCCGCCGCTTTCATCACGGCGcac                                                                                                                                                                                                                >  1:370276/1‑238 (MQ=255)                           ccgaggaacgtGGGCGTCGTATTCGCCCCGTCAAT‑CTTCGGTCGCTTATCTTTTCAACGCCGGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAC‑A‑CCTCGACACTAGTCCGCCGCTTTAATCACGGCGCACAACCg                                                                                                                                                                                                           >  1:537935/11‑238 (MQ=255)                           ccGTGGGATATGGGCGTCGTATTCGTCCCGCCAAT‑CTCCGGTCGCTAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCg                                                                                                                                                                                                           >  2:611297/1‑238 (MQ=255)                           ccGTGGGATATGGGCGTCGTATTCGTCCCGCCAAT‑CTCCGGTCGCTAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCg                                                                                                                                                                                                           >  2:894668/1‑238 (MQ=255)                           ccGTGGGATATGGGCGTCGTATTCGTCCCGCCAAT‑CTCCGGTCGCTAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCg                                                                                                                                                                                                           >  2:103863/1‑238 (MQ=255)                                     tGGGCGTCGTATTCGTCCCGCCAAT‑CTCCGGTCGCTAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTc                                                                                                                                                                                                >  2:179916/1‑239 (MQ=255)                                     tGGGCGTCGTATTCGTCCCGCCAAT‑CTCCGGTCGCTAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTc                                                                                                                                                                                                >  1:842922/1‑239 (MQ=255)                                     tGGGCGTCGTATTCGTCCCGCCAAT‑CTCCGGTCGCTAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGAGCACAACCGCCTGTGCAGTc                                                                                                                                                                                                >  1:641251/1‑239 (MQ=255)                                          gtcgtATTCGTCCCGCCAAT‑CTCCGGTCGCTAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGc                                                                                                                                                                                             >  2:720986/1‑237 (MQ=255)                                           tcgtATTCGTCCCGCCAAT‑CTCCGGTCGCTAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTCAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTCGCCCGCCGCTTTAATCACGCCGCACAACCGCCTGTGCAGTCGGCCCt                                                                                                                                                                                          >  2:739985/1‑239 (MQ=255)                                           tcgtATTCGTCCCGCCAAT‑CTCCGGTCGCTAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCt                                                                                                                                                                                          >  2:948857/1‑239 (MQ=255)                                                   ctcccccccaccctcCGTTCGATATTTTTTACAACGCCGGG‑CCCCGCGGGGTG‑TGTTTTTTTTAACCTCCAGCGGGTTTAAAATATTTT‑CA‑AGAAGTTCTTCGGGAGGCCG‑CACCCATAACACAGGC‑AACCCGAGCCGAACCCTTGTACAACCCCCGTTTTAAACACCCGAAA‑C‑CCTCGCCGCAAGCCCGCCGCTTTAATCCCGCCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGt                                                                                                                                                                                   <  1:730521/227‑1 (MQ=255)                                                   ctCCCGGCAATCCCCCGGTCGCTATTTTTTACACCGCCTGG‑CACTGCCGGGTTTTGTTTTTTTAAACTTCAGGAGGT‑TTAAAATATTTC‑CC‑AGTAAGTATTCGGGAGGCTG‑CACCCATGACACAGACAAACCTGAG‑CAAACCCCTTTTCAACCCCCGTTTTAAACATCCTAAA‑C‑CCTCGACGCTAGTCCGCCTCTTTAACCCCGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGt                                                                                                                                                                                   <  2:1117476/238‑1 (MQ=255)                                                         ccAAT‑CTCCGGTCGATAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAACCATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCCCGGCGCACAACCGCCTGCGCAGCCGGCCCTTGATGGAAAAACCa                                                                                                                                                                            >  1:531520/1‑239 (MQ=255)                                                           cacgtctgcgggtcttattttttCCACGCCTGGCCTTTGCCGGGCTTTTTTTTTTTACCCTCCAGGGGGG‑TACAAATATTTG‑CA‑AGAAAGTGTTCTGGAGGCAT‑CACCCAGAACAAAGGAAACCCGGAG‑CGAACCCGTTTTC‑ACCCCCCCTTAAAAACACCTGAACC‑CCCCGCTTTTAGCCCGCCTCTTTAACCCCGCCCCACACCCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCa                                                                                                                                                                            <  1:1081180/220‑1 (MQ=255)                                                                  cGGTCGCTAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTc                                                                                                                                                                      >  2:719861/1‑237 (MQ=255)                                                                  cGGTCGCTAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCAGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTc                                                                                                                                                                      >  2:138657/1‑237 (MQ=255)                                                                     gcgcatattttgtCACCGCTTGG‑CACGGCGGGGCTTTTTTTTTTTTACCTACAGGCGGT‑TTA‑AATAATTTCCC‑GGAAAGTGTCCTGGAGCCTG‑CAACCATAACACAGGCAACCCTGAG‑CGACCCCTTGTACAAACCCCGTTTAAAACACCCAGAA‑C‑CCGCGCCGCTACCCCGCCGCTTAAACCACGGCCCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTg                                                                                                                                                                  <  1:720986/226‑1 (MQ=255)                                                                                 tCAACGCCTGT‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTGTGGAGGCTG‑CATCCATGTCACAGGCAAACCTGAG‑CGATACTCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGGCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGTCCTTAATGGTAATACCCTCCCTCACTGGTATCGCATGAtt                                                                                                                                                     >  1:141162/1‑239 (MQ=255)                                                                                       ccTGG‑CACTGCCGGGCGTTTTTCTTTTAAACTTCAGGCGGG‑TAAAAATATTTT‑CC‑AGTAAGTATTCGGGAGGCTG‑CACCCATGACACAGGCAACCCTGAG‑CAAACCCCTTTTAAACCCCCGTTTAAAACATCCTGAA‑C‑CCCCGACGCTAGTCCGCCGTTTTAATCCCGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGt                                                                                                                                               <  2:215316/239‑1 (MQ=255)                                                                                        ccgggccctcccgtgtttttttttttttAACTTGAGGGGGG‑TAAAAATATTTC‑CC‑AGAAATTTTTGGGGGGGCTG‑CAACCAGGACCCAGGAAACCAGGAG‑CAAACCCCTGTCCACCCCCCGCTTAAAACACCCGAAA‑C‑CCCCGACGCTGGCCCCCCGTTTTAACCACGGCCCACAGCCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGt                                                                                                                                               <  1:611297/216‑1 (MQ=255)                                                                                         ctgggcctcccGGGGGTTTTTTTTTTTTACCTCCGGGGGG‑TTAAAAATGTTT‑CCAAGAAGGTCTCGTGGAGGTCC‑CACCCGGCACACAGGCAACCGGGAC‑CAACCCCCTTTACAACCCCCGTTTAAAACCCCCTAAA‑C‑CCCCGCCGCTGGCCCCCCGTTTTAATCCCGGCACACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGt                                                                                                                                               <  1:652236/230‑1 (MQ=255)                                                                                                  ccGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTTATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGa                                                                                                                                     >  1:41103/1‑239 (MQ=255)                                                                                                  ccGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGa                                                                                                                                     >  2:802554/1‑239 (MQ=255)                                                                                                         ttgttTTTTTAAACTTCAGCCGGT‑TCACAATGGTTC‑CT‑AGTATGTTTGCGGGATGCGA‑CACCCATGAAACCGCAAACCCTGAG‑CAACCCCCTGTTCAACCCCCGTTTTAAACTTCCTGAA‑C‑CCCCGACGCTGGCCCGCCGCTTTAATCCGGGCCCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGgcg                                                                                                                              <  1:1220078/239‑1 (MQ=255)                                                                                                                               ggtttAAAATATTTT‑CC‑AGTAAGTATTCGGGAGTCTG‑CACCCAAGCCACAGGCAAACCTGAG‑CGAACCCCTGTCCAACCCCCGCTTAAAACATCCTGAA‑C‑CCTCGACGTTAGCCCCCCGTTTTAACCCCGGCCCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGaaa                                                                                                          <  2:339971/235‑1 (MQ=255)                                                                                                                                g‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATc                                                                                                        >  1:697492/1‑238 (MQ=255)                                                                                                                                  ttACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCa                                                                                                                                                                     >  2:1198198/1‑176 (MQ=255)                                                                                                                                  ttACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCAGCTTTAATCACGACGCACAACCGCCTGTGCAGTCGGCCCTTGATGGGAAAACCATCCCTCAATGGTATCGCAAGATTAACCGGCTGGTGTGGCTCTGGCGCGGCATTGACCCACGCGAAATCCt                                                                                                      >  1:808917/1‑239 (MQ=255)                                                                                                                                   aaaaaTAGTTC‑CA‑AAAAATTATTCTGG‑GGCTGCCACCCAAGACACAGCAAACCCTGAG‑CGAACCCCT‑TTTAAACCCCCGTTTAAACCACCAAAA‑ACCCTCGCCCTTGGCCCCCCGCTTTAACCCCGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTc                                                                                                     <  2:1078618/236‑1 (MQ=255)                                                                                                                                     aacaatgtttccaaataagtcTTCAGGGGGCTC‑CTCCCACAACACACGCAACCCGGAC‑ACAACCCCTCTTCAACCCCCTTTTAAAACCCCCTAAA‑C‑CCTCCCCCCGACCCCGCCGTTTAACTCACGCCCCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTc                                                                                                     <  1:894668/224‑1 (MQ=255)                                                                                                                                       atggTTT‑CC‑AGTGAGTATTCTGGAGACTG‑CATCCATGACACAGACAAACCTGAG‑CGAAACCCTATTCAAACCCCGCTTCAAACATCCCAAA‑A‑CCACGACGATAGTCCGCAGCTTTCATCACGGCCCACACCCGCCTCTGCAGTGGGCCCTTGATGGTAAAACCATCCCTCACCGGTACCGCATGCTTAACCGTCTGATGTGGATCTCGCGCGGCCTACACCCCCGCGAAATctacgag                                                                                                  >  1:29045/4‑232 (MQ=255)                                                                                                                                           ttt‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCa                                                                                             >  2:413234/1‑239 (MQ=255)                                                                                                                                           ttt‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCCCGGCATTGACCCACGCGAAATCCTCGACGTCCa                                                                                             >  1:112303/1‑239 (MQ=255)                                                                                                                                            tt‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGCTTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGACATCCTCGAccgcca                                                                                             >  1:573488/1‑233 (MQ=255)                                                                                                                                            tt‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAg                                                                                            >  1:477857/1‑239 (MQ=255)                                                                                                                                             t‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGc                                                                                                                                                           >  2:115657/1‑175 (MQ=255)                                                                                                                                             t‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAg                                                                                            >  2:502675/1‑238 (MQ=255)                                                                                                                                             t‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAg                                                                                            >  2:674/1‑238 (MQ=255)                                                                                                                                               cc‑ATTAAGTGTTGGGGGGGCTA‑CACCCAAGACACAGGAAACCCAGAG‑CAAAACCCTGTCCACCCCCCGTTATAAACCTCCTGAA‑C‑CCTCGCCGTTAGTCCGCCTCTTTAACCCGGCCGCAAAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGc                                                                                          <  1:502675/239‑1 (MQ=255)                                                                                                                                                     aagaagtgtGGAGTCTG‑CACCCAGGACCCAGGAAAACCCGCG‑CGAACCCCTGTACAGCCCCCTGTATAAAC‑CCCAGAA‑ACCCCCGCCGCGAGCCCGCCGTTTAAACCGGGGCACAAAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGATGTCCAGGCACGTa                                                                                     <  1:938360/231‑1 (MQ=255)                                                                                                                                                          ttCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATtgtg                                                                                >  2:600107/1‑239 (MQ=255)                                                                                                                                                                  gCTG‑CATCCATGACACAGGCAACCCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGCTGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGa                                                                        >  1:802187/1‑239 (MQ=255)                                                                                                                                                                  gCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGCCGTCCAGGCACGTATTGTGCTGAGCGa                                                                        >  1:923095/1‑239 (MQ=255)                                                                                                                                                                  gCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGa                                                                        >  2:130317/1‑239 (MQ=255)                                                                                                                                                                    tG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCCCGTATTGTGATGAGCGAt                                                                       >  1:624040/1‑238 (MQ=255)                                                                                                                                                                    tG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGAt                                                                       >  2:131171/1‑238 (MQ=255)                                                                                                                                                                    tG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGAt                                                                       >  2:1073033/1‑238 (MQ=255)                                                                                                                                                                    tG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGAt                                                                       >  2:878941/1‑238 (MQ=255)                                                                                                                                                                    tG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGCGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGCCGTCCAGGCACGTATTGGGATTAGCGAt                                                                       >  2:478905/1‑238 (MQ=255)                                                                                                                                                                       cATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGcc                                                                    >  1:459129/1‑239 (MQ=255)                                                                                                                                                                       cATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCCCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGAATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGCCTTTGACCCACGCGAAATCCTCGCCGTCCAGCCACGAATTGGGATGAGCGATGcc                                                                    >  2:1158098/1‑239 (MQ=255)                                                                                                                                                                        aTCCAGGACACAGAAAAACCTGAA‑CGAACCCCTGTTCAACCCCCGTTTAAAACATCATGAA‑C‑CCGCGACGCTGTCCCGCCGATTAAACCAGGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCg                                                                   <  2:537539/239‑1 (MQ=255)                                                                                                                                                                             tGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGt                                                              >  2:1043644/1‑239 (MQ=255)                                                                                                                                                                                    ggCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGCCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGCCTCGCATGCTTAACCGTCTGATGTGGATCTGGCGCCGCATTGACCCACGCGAAATCATCAACGTCCAGGCACGGATTGTGCTGCGCCATTCCGAACGTACcgac                                                        >  1:656594/1‑238 (MQ=255)                                                                                                                                                                                      gcaaccctgccacacccccTGTACAACCCCCTCTAAAAACACCCCAAA‑C‑CCGCGCCGTTGCCCCCCCTCTTAAACCCCGGCCCCCAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCGCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACcgacg                                                       <  1:1158098/222‑1 (MQ=255)                                                                                                                                                                                          ccTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACCGACGATGAtt                                                 >  2:1144900/1‑239 (MQ=255)                                                                                                                                                                                             gAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACCGACGATGATTtat                                              >  2:200067/1‑239 (MQ=255)                                                                                                                                                                                              aG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGAGATGCCGAACGTACCGACGATGAgttct                                              >  1:1063909/1‑236 (MQ=255)                                                                                                                                                                                               g‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACCGACGATGATTtatat                                            >  2:874006/1‑238 (MQ=255)                                                                                                                                                                                               g‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACCGACGATGATTTatac                                            >  2:317141/1‑239 (MQ=255)                                                                                                                                                                                               g‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGAGGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACCGACGATGATTTatac                                            >  2:126807/1‑239 (MQ=255)                                                                                                                                                                                                           ttCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGAc                                                                                                                    >  2:428343/1‑156 (MQ=255)                                                                                                                                                                                                           ttCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACCGACGATGATTTATACGATACGGTGAt                                 >  2:361969/1‑239 (MQ=255)                                                                                                                                                                                                                     gCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCCCGTATTGTGATGAGCGATGCCGAACGTACCGACGATGATTTATACGATACGGTGATTGGCTACCGt                       >  2:174681/1‑239 (MQ=255)                                                                                                                                                                                                                     gCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACCGACGATGCTTTATACGATACGGTGATTGGCTACCGt                       >  1:264801/1‑239 (MQ=255)                                                                                                                                                                                                                     gCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACCGACGATGATTTATACGATACGGTGATTGGCTACCGt                       >  2:708810/1‑239 (MQ=255)                                                                                                                                                                                                                     gCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACCGACGATGATTTATACGATACGGTGATTGGCTACCGt                       >  1:638392/1‑239 (MQ=255)                                                                                                                                                                                                                     gCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACCGACGATGATTTATACGATACGGTGATTGGCTACCGt                       >  1:1038144/1‑239 (MQ=255)                                                                                                                                                                                                                     gCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACCGACGATGATTTATACGATACGGTGATTGGCTACCGt                       >  2:365157/1‑239 (MQ=255)                                                                                                                                                                                                                     gCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACCGACGATGATTTATACGATACGGTGATTGGCTACCGt                       >  2:347986/1‑239 (MQ=255)                                                                                                                                                                                                                     gCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACCGACGATGATTTATACGATACGGTGATTGGCTACCGt                       >  2:130081/1‑239 (MQ=255)                                                                                                                                                                                                                     gCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGCCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACCGACGATGATTTATACGATACGGTGATTGGCTACCGt                       >  1:1029035/1‑239 (MQ=255)                                                                                                                                                                                                                        taaaaCACCCTAAC‑C‑CCCCGCCGCTGGCCCCCCGCTTAAACCAGGGCGCAAATCCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACCGACGATGATTTATACGATACGGTGATTGGCTACCGTggc                    <  2:537935/237‑1 (MQ=255)                                                                                                                                                                                                                                 tccgtaacccTCGCCGCTATGCCGCGTCTTAACCCCCGCCGCACACCCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACCGACGATGATTTATACGATACGGTGATTGGCTACCGTGGCGGCAACt             <  1:1039995/231‑1 (MQ=255)                                                                                                                                                                                                                                    aa‑a‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACCGACGATGATTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGAtt        >  2:1133145/1‑239 (MQ=255)                                                                                                                                                                                                                                      aacccTCGCCGCTAGCCCGCCCTTATAACCGCGCCCCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACCGACGATGATTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGAtt        <  1:361969/236‑1 (MQ=255)                                                                                                                                                                                                                                            cGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACCGACGATGATTTATACGATACGGGGATTGGCGACCGTGGCGGCAACTGGATTTATGAg  >  1:643775/1‑239 (MQ=255)                                                                                                                                                                                                                                                gCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACCGACGATGATTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTAt     >  2:35449/1‑232 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                      TCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAAT‑CTCCGGTCGCTAATCTTTTCAACGCCTGG‑CACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGG‑TTACAATAGTTT‑CC‑AGTAAGTATTCTGGAGGCTG‑CATCCATGACACAGGCAAACCTGAG‑CGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAA‑A‑CCTCGACGCTAGTCCGCCGCTTTAATCACGGCGCACAACCGCCTGTGCAGTCGGCCCTTGATGGTAAAACCATCCCTCACTGGTATCGCATGATTAACCGTCTGATGTGGATCTGGCGCGGCATTGACCCACGCGAAATCCTCGACGTCCAGGCACGTATTGTGATGAGCGATGCCGAACGTACCGACGATGATTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAG  >  NC_000913/256362‑256826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑