Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,478,418	G→T	12.9%	A65S (GCG→TCG)	ynbB →	putative CDP‑diglyceride synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,478,418	0	G	T	12.9%	296.6 / 6.8	94	A65S (GCG→TCG)	ynbB	putative CDP‑diglyceride synthase
Reads supporting (aligned to +/- strand):  ref base G (26/55);  new base T (11/2);  total (37/57)
Fisher's exact test for biased strand distribution p-value = 4.89e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGTTTTCTGCATACCGTGAGCGCCATTTTTTGGGG  >  NC_000913/1478194‑1478647                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                       cGGAGTGTGCTTCTTATGAGCTCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGtttt                                                                                                                                                                                                                          <  2:1054135/239‑1 (MQ=255)        tgCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTATGGGGGG‑GAAAAGGGGG‑GGGGGTATAATACGTATTTGTATCTGGTGGGGGATCGTGCTTTGTTTTTTTTTTGTTATTTTAGGTGCACGCTGGGTGACGTTTACGCTTTTTTTGTTGTTTAGGTTTCT‑gtcgt                                                                                                                                                                                                                  >  1:202443/1‑235 (MQ=255)        tgCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCAt                                                                                                                                                                                                                  <  2:1210420/239‑1 (MQ=255)        tgCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑GTACCGGGGC‑GGAAAAGGGGGGAGGGTACATTACGTATTCGTTACTGGTGGGGCGTCGTCATTTGTTTTTTATTGGTGTTTTTAGGTGCATCCTGGCTTAGGCTATGGTTTTTTGCGCTGGTAACttttttcgtct                                                                                                                                                                                                                   >  1:995483/1‑232 (MQ=255)         tcttcttATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCAt                                                                                                                                                                                                                  <  1:1099413/237‑1 (MQ=255)         gCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCAt                                                                                                                                                                                                                  <  2:109902/238‑1 (MQ=255)           ttctgATGAGCGCTGAAAGAGAAATCTGGAAAAATCTCTGGCATCACTTTTCGCGTTGTTAAGTTTAGCAACGCTGATAAACCGTTTTCTGCGATGGCGG‑TAACCGGAGA‑GAAAAGGGGGGAAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGGCATTTGTTTTTCAATGGTGAATTCAGGTCCACGCTGGATGACGTTGACGTTATTTGCGCTGATAAGTTTGCT‑GGCATTgt                                                                                                                                                                                                               <  1:328174/239‑2 (MQ=255)            tcttATGATCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACCCTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGa                                                                                                                                                                                                               <  1:417609/238‑1 (MQ=255)              ttATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGG‑GAAAAGGGGGGGAGGTTACATTTCGTATTCGTACCTAGTGGGGGATCGGCATTTGTTTTTTTTTTGTGATTTTATGTCCACGGTGGCTGGCGCTTAGGTTTTTTTTGCTTGTATGTTTTTT‑TGCATTGAAAg                                                                                                                                                                                                            >  1:38646/1‑239 (MQ=255)               tATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑GTACTGGGGAGGAAAAGGGGGGGGGGGTTCATTTATTGTT‑GTATCTTGTTGGGCAACGGCTTTTGTTTTTTTATGGGGTTTTTCGGACCCCGCTGGGTGGCGGTTTCGGTTTTTTTGCTGATGTGGTTTTT‑TGCATTGtaaaa                                                                                                                                                                                                           >  1:573757/1‑237 (MQ=255)                  gAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAata                                                                                                                                                                                                        <  2:346535/239‑1 (MQ=255)                     cgcTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTAACGGGGA‑GAAAAGGGGGGGGGGGTTAATTATGTATTCGGTACTGGGGGGGGCTCGTCATTTGTTTTTTAATTGGTATTTTTGGTGCCCGCTGGGCGATGTTGAGGTTTTTTTTGCGTGTAAGTTTTTT‑GGCATTGTAAAGATAttt                                                                                                                                                                                                     >  1:731212/1‑238 (MQ=255)                      gcTGAAATATAATGCTGGAAAAATCTCTGGCAACACTGTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTg                                                                                                                                                                                                     <  2:409793/238‑1 (MQ=255)                           aaTATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCTATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGt                                                                                                                                                                                                    <  2:26064/234‑1 (MQ=255)                                 aTGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATc                                                                                                                                                                                         <  2:816/239‑1 (MQ=255)                                  tGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTTCCAGGGG‑GAAAAGGGGGGGGGGGTTCATTAAGTATTCGTCTCTTGTGGGGGGTCGTCATTTTTTTTTTAATGGTGTTTTTTTGTTCACGCTGGGTGAGGTTTACGTTTTTTTTGCTGATTAGGTTTTT‑TGCCTTGATAGAATATTTTTCGCtttttttt                                                                                                                                                                                        >  1:998259/1‑233 (MQ=255)                                  tGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGGGA‑GAAAAGGGGGGGTGGGTACATTACGAATTCGTACCTTGTGGGGCGACGTCATTTGTTTTTTATAGGTGATTTGAGTTCAACGCTGGGGTACGGTTTCGTTTTTTTTGTTGCTAACTTTTCT‑TGCCTTGTAGGAATAttttttcgtttttttt                                                                                                                                                                                        >  1:923687/1‑225 (MQ=255)                                     tGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTTCTCCCGG‑GAAAAAGGGGGGAGGCTACATTTCGTTCCCTGTCCTGCTGGGGCATCCCCACTTTTTGTTCTCTTGGGCTTTTAGCTCGACCCTCGGCCACCTTGACGTTTTTTTCGGCGATAACTTTTCT‑TTCGTTGAAAGAATAtttgttgctttttttcttg                                                                                                                                                                                     >  1:26064/1‑222 (MQ=255)                                               tctGGCAACACTTTTCGAGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGGCATTTGTTTTGCAATGGTGATTTCAGTTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGGATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCcgg                                                                                                                                                                           <  2:151055/239‑2 (MQ=255)                                               tctGGCAACACGTCTCGCGTTGTTAATTTTAGCAACGGTGATAAACCGTTTTCTGGTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGAGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTCTTAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCcgc                                                                                                                                                                           <  1:484365/239‑1 (MQ=255)                                                ctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACTTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCcgcg                                                                                                                                                                          <  2:661123/239‑1 (MQ=255)                                                ctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCcgcg                                                                                                                                                                          <  2:301005/239‑1 (MQ=255)                                                ctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCcgcg                                                                                                                                                                          <  2:1083403/239‑1 (MQ=255)                                                ctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCcgcg                                                                                                                                                                          <  2:301500/239‑1 (MQ=255)                                                ctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCcgcg                                                                                                                                                                          <  2:387149/239‑1 (MQ=255)                                                ctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCcgcg                                                                                                                                                                          <  1:752229/239‑1 (MQ=255)                                                ctGGCAACACGTTTCGCGTTGTTAAGTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTCGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCcgcg                                                                                                                                                                          <  2:799656/239‑1 (MQ=255)                                                ctGGCAACAATTTTCGCGGTGTTTATTTTATCAACGCTGATAAACCGTGTTCGTGTATTGCGG‑TTTCCCGATA‑GAAAAAGGGGTGAGGTTACATTGCGTATTCGTACCTGGTGCGTCATCGTCATGTGTGTTTCAATGGTAATTTCAGTTCCACGATGGATGACGTTCGCGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTGCcgcg                                                                                                                                                                          <  1:497225/239‑1 (MQ=255)                                                 tGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCcgcg                                                                                                                                                                          <  1:1007019/238‑1 (MQ=255)                                                 tGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGt                                                                                                                                                                         <  2:779471/239‑1 (MQ=255)                                                         cTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTtata                                                                                                                                                                 <  1:1032872/239‑1 (MQ=255)                                                           tttCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGGGG‑GAAAAGGGGGGGAGGGTTAATTACGTTTTTGTACCTGGTGGGGGAGCGTCATTTGTTTTTTTATGGTGGTTTTTTGTCCCCGCTGGGTGGAGTTGTCGTTTTTTTTGCTGTTAAGTTTTTT‑GGGCTTTTAAAAATATTGTTAGCTTTTTTTTGTTCATTTTTTGCGCTGGttttttt                                                                                                                                                               >  1:898805/1‑234 (MQ=255)                                                            ttCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTg                                                                                                                                                              <  1:1205835/239‑1 (MQ=255)                                                                 gttgttAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTa                                                                                                                                                         <  2:573096/239‑1 (MQ=255)                                                                   tgttAATTTTAGCAACGGTTATAAACCGGTTTTTTCTATGGGTG‑TTACCGGACA‑GACACGGGGGTGTGGTTAGCTTAGTTATTCCTTCCTTGTGGGTCTTGGTCGTTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTAGCGTTTTTTTCGCTGATAATTTTTCT‑GGCATTGAAATAATATTGTACTCTTATATCTGTACATTTTCCGCTTTGGTTATATTGGGGTAtt                                                                                                                                                       <  1:25546/239‑1 (MQ=255)                                                                   tgttAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTAtt                                                                                                                                                       <  2:373739/239‑1 (MQ=255)                                                                         ttttAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGTTGAGGTTTCATTACGTATTCGTACCTGGTGTGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCtt                                                                                                                                                 <  2:779193/239‑1 (MQ=255)                                                                         ttgtAGGAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTAGCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATTGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTTTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCtt                                                                                                                                                 <  1:153451/236‑1 (MQ=255)                                                                          tttAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTTCGTATTCGTACCTGGGGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTGTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTa                                                                                                                                                <  2:614883/239‑1 (MQ=255)                                                                             aGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GGAAAGGGGGGGGGGGTACATTACGTATTTGTACCCGGTGGGGCCTCGTCATTTTTTTTTTTATGGTGGTTTTAGGGCCCCGCTGGATGGCGTTGACGGTTTTTTTGCTGATAAGTTTTTTGGGCTTTGAAAAATAATTTTACG‑TTTTATTTGTACCTTTTTCGCGTTTGTTATTTTTGGGTATTTCTCTTTACt                                                                                                                                             >  2:931624/1‑239 (MQ=255)                                                                             aGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAAGGGGGGGGGGTTCATTACGTATTCGTACCTGGGGGGGCATCGTCCTTTTTTTTTTTATGGGGGTTTTTGGTCCACGCTGGGTGACGTTTTCGTTTTTTTTGCTGGTAAGGTTTTT‑TGCATTTGAAGAATATTTTACGCTTTTATCTTTACATTTTTCGCGTTGGTTATATTTGGGGTTTCCTCTTAACt                                                                                                                                             >  2:259193/1‑239 (MQ=255)                                                                                  cGCTGATAAACCGTTTTCTGCTATGGCGG‑TTAACGGAGA‑GAAAAAGGGGGGAGGGTAATTTTGGTGTTTTTTCCGCGGGGGGGAACGTCCTTTGTTTTTCACAGGTGATTTTAGTGCCAAGCTGTGTGAAGTTTAAGGTTTTTGCGCTGATATGTTTTTT‑TGTATTGTTAGGATATTGTAAGCTTTTATCTGTGCACTTTCCGCGTTGGGGATATTTTGGTAttttccttgatcttttt                                                                                                                                        >  1:637113/1‑224 (MQ=255)                                                                                               ttttCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGGGGGGGTAAATTACGTATTCGTACCTGGGGGGGGATCGTCATTTTTTTTTTAATGGGGGTTTTAGGGCCACGCTGGATGACGTTGTCGTTTTTTTCGCTGGTAAGTTTTTT‑GGGATTTTAAGAATATTTTACGCTTATATTTGTACATTTTTTGCGTTGGGTATATTGGGGTATTTTTCTTTACTATTTTCTTATTGGttttt                                                                                                                           >  2:322248/1‑238 (MQ=255)                                                                                                tttCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTaa                                                                                                                          <  2:546735/239‑1 (MQ=255)                                                                                                     gCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCt                                                                                                                     <  1:171712/239‑1 (MQ=255)                                                                                                     gCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCt                                                                                                                     <  1:1112833/239‑1 (MQ=255)                                                                                                            cGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTTCACGCTGGATGACGTTGACGTTTTTTTCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGc                                                                                                               <  2:637113/238‑1 (MQ=255)                                                                                                                    cGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTg                                                                                                       <  1:420874/239‑1 (MQ=255)                                                                                                                    cGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTg                                                                                                       <  2:998259/239‑1 (MQ=255)                                                                                                                                 ggggTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTa                                                                                                                                    >  1:1106750/1‑198 (MQ=255)                                                                                                                                 ggggTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTa                                                                                                                                    <  2:1106750/198‑1 (MQ=255)                                                                                                                                      gatgTTACATTACGTATTCTTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGc                                                                                      <  1:852616/236‑1 (MQ=255)                                                                                                                                      gAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTg                                                                                        >  1:340178/1‑237 (MQ=255)                                                                                                                                      gAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTg                                                                                        >  1:441735/1‑237 (MQ=255)                                                                                                                                          ttACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTg                                                                                    <  1:55546/237‑1 (MQ=255)                                                                                                                                           tACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGt                                                                                                            >  2:166552/1‑212 (MQ=255)                                                                                                                                           tACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGt                                                                                                            <  1:166552/212‑1 (MQ=255)                                                                                                                                              aTTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTc                                                                              <  2:202443/239‑1 (MQ=255)                                                                                                                                              aTTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTc                                                                              <  2:38646/239‑1 (MQ=255)                                                                                                                                                  cGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGat                                                                          >  1:942658/1‑239 (MQ=255)                                                                                                                                                        cGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCGGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGaaa                                                                                                                                                                                                             >  1:434584/1‑102 (MQ=255)                                                                                                                                                        cGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCGGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGaaa                                                                                                                                                                                                             <  2:434584/102‑1 (MQ=255)                                                                                                                                                        cGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGc                                                                    <  2:244585/239‑1 (MQ=255)                                                                                                                                                           aCCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCa                                                                  <  1:1191673/238‑1 (MQ=255)                                                                                                                                                            ccTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCAcc                                                                >  2:824398/1‑239 (MQ=255)                                                                                                                                                              tggtggGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCgg                                                              >  2:1014059/1‑239 (MQ=255)                                                                                                                                                               ggtggGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCgg                                                              >  2:186255/1‑238 (MQ=255)                                                                                                                                                                 tggGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCa                                                           >  2:299102/1‑239 (MQ=255)                                                                                                                                                                 tggGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATAGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGTCa                                                           >  2:1198410/1‑239 (MQ=255)                                                                                                                                                                   gggCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCa                                                                                                                                                                                                                   <  2:539304/85‑1 (MQ=255)                                                                                                                                                                   gggCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGCTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTATTTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAg                                                         >  1:905908/1‑239 (MQ=255)                                                                                                                                                                   gggCATCGTCATTTGTATTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCa                                                                                                                                                                                                                   >  1:539304/1‑85 (MQ=255)                                                                                                                                                                     gCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGCTATTAGCCACCGGGCAAGtt                                                       >  1:555142/1‑239 (MQ=255)                                                                                                                                                                        tCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTa                                                    <  2:731212/239‑1 (MQ=255)                                                                                                                                                                         cGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAg                                                   <  2:544578/239‑1 (MQ=255)                                                                                                                                                                          gtgaTTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGATATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGt                                                  <  1:54124/236‑1 (MQ=255)                                                                                                                                                                                tGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTg                                             >  2:920745/1‑238 (MQ=255)                                                                                                                                                                                   ttttCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGAccc                                         <  2:441735/239‑1 (MQ=255)                                                                                                                                                                                      tCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTTTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCctc                                      <  2:923687/239‑1 (MQ=255)                                                                                                                                                                                      tCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCctc                                      <  1:322248/239‑1 (MQ=255)                                                                                                                                                                                          tGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGt                                  <  2:299347/239‑1 (MQ=255)                                                                                                                                                                                           ggTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGtt                                 <  2:289348/239‑1 (MQ=255)                                                                                                                                                                                            gTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGttt                                >  2:97042/1‑239 (MQ=255)                                                                                                                                                                                               aTTTCAGTTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGTGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGTTGACCCCTCTGGttt                                >  1:1158923/1‑236 (MQ=255)                                                                                                                                                                                               aTTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGttt                                >  2:608078/1‑236 (MQ=255)                                                                                                                                                                                                   cAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGTTTTCTGCAt                         <  2:898805/239‑1 (MQ=255)                                                                                                                                                                                                      gTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGTTTTCTGCATAcc                      >  2:1012569/1‑239 (MQ=255)                                                                                                                                                                                                         cACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGTTTTCTGCATACCGTg                   >  1:473897/1‑239 (MQ=255)                                                                                                                                                                                                           cGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGTTTTCTGCATACCGTGa                  >  2:644411/1‑238 (MQ=255)                                                                                                                                                                                                                 aTGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGTTTTCTGCATACCGTGAGCGCCAt           >  2:908882/1‑239 (MQ=255)                                                                                                                                                                                                                     cgttgacgttTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGTTTTCTGCATACCGTGAGCGCCAttttt       >  2:1035274/1‑239 (MQ=255)                                                                                                                                                                                                                          acgttTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCctc                                      <  2:459144/203‑1 (MQ=255)                                                                                                                                                                                                                          acgttTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCctc                                      >  1:459144/1‑203 (MQ=255)                                                                                                                                                                                                                           cgttTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGTTTTCTGCATACCGTGAGCGCCATTTTTTgggg  <  1:259193/238‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                       CGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGTTTTCTGCATACCGTGAGCGCCATTTTTTGGGG  >  NC_000913/1478194‑1478647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑