Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,151,689 G→T 40.5% intergenic (+43/+22) yegJ → / ← yegK uncharacterized protein/ser/thr phosphatase‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,151,6890GT40.5% 12.4 / 13.2 40intergenic (+43/+22)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base G (14/9);  new base T (6/10);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01

TGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATG  >  NC_000913/2151496‑2151925
                                                                                                                                                                                                           |                                                                                                                                                                                                                                                
tGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTTAACATTTATTCCTTTTTTGTCACCCAATCCAAAAAAGAAAGCGACGAGCTAAAAGGAAAAATAATTTGCTTATCAACCGGTTTATTTTTTTTTTTAAATTGGGGGTTTGATTTTTTTTTTTAACACCCCGTAAAAAACACACTTTCCCcacacccagcg                                                                                                                                                                                                    >  2:477713/1‑231 (MQ=255)
              aaaaCAAAAAAGAAGATATTTCAGCCTGGATGTATACGCGGGGGGATAAAATTTACGGCGTTTATCCCATTGATCCTTTATTGGTCCCCTACCCAAAAGAAGAAGCCGACGAGCTAAGAGAAAAATTAGTTCGCTAATCACCCGTTATATTTATTGTTTAAACTGGCGTTTGATTTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcat                                                                                                                                                                                   <  2:436470/239‑1 (MQ=255)
                    aaaaaaaaGATATTTCAGCTGGGATTTATGCGGGGGGGAAAAAAATTACCGGGGTTTATACCATTACTCTTTTTTGGTTCCCCTAACAAAAAGAAAAAGCCGAGGGGAAAAGAAAAAAATTTGTTCGCTATCCGCCTTTTATATTTTTTGTTAAAACGGGGGTTTAATTTGTTGTTTCAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCg                                                                                                                                                                             <  1:1077744/237‑1 (MQ=255)
                                   cagatggtttGTATCCGCGGGGGGATAAAATTTGCGGCGTTTATACCATTTATCTTTTATTGTTCCCCTATCAAAAAGAAGAAGCCGACGAGATAAGAGAAAATTTAGTCCGTAACTCAGCCTTTATTTTTTTTTTTTAACCGGGCGTTTGATTTTTTTTCTACAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTAccg                                                                                                                                                              <  1:477713/230‑1 (MQ=255)
                                                  cgcgggGGAAAAAAATTAAGGGGGTTTATACAATTGACCTTTTATGGTCCCCCTACCAAAAAAAAGAAGCCGAGGAGCTAAGAGAAAATTTATTCCCTAATTCAGCCTTTATTTTTTTTTTTAAAACGGGGGGTTAATTTTTTGTCTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACaaaa                                                                                                                                               <  2:653900/239‑1 (MQ=255)
                                                                  ttACGGGGTTTTACCAATTGTCCTTTTATTGGCCCCCTACCAAAAAAAAGAGCCCAAGAACATAAAACAAAATTTAGTCCGTAAATCAGCTTTTTTATTTTTTTTTAAACCGGGGGGTTAATTTTTTTTCTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTc                                                                                                                               <  2:614736/239‑1 (MQ=255)
                                                                  tgaggggggtacaaCCATTGATCTTTTATGTTCCCCTAAACAAAAAGAAGAGCCGGCAGAGAAAAGAAAAAATTTGGTCCATAAACAGGCGTTTATTTTTTTTTTTAAACCGGGCGTTTGATTTGTGGTTTTCATCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTc                                                                                                                               <  1:361039/226‑1 (MQ=255)
                                                                                     tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCCTGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTTTTATCATCCGTACGCCCGGTTACCCCCGGGCTGGACAAAAACTGCTTTAACAATTCAg                                                                                                                             >  2:463056/1‑223 (MQ=255)
                                                                                     tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGGAACTCAATCTGCCCACAGAGCCAGTGCCCACGGTTTATCATCATCCGTACGGCCGTTTCACGCCGGGCTGGACAAAAACTGCTTTAACAAATCAGGTAATAAAACGAggtt                                                                                                             >  2:793499/1‑236 (MQ=255)
                                                                                     tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCAATCTTCCCACAGCGCCCGTGCCAACGGTTTTTCATCCTCCGTCCGCTCGTTTACCGCCGGGCTGGACAAAAACTGCCTTAAAAATTCAGGGAAAAAAACGAgttg                                                                                                             >  2:895467/1‑239 (MQ=255)
                                                                                      gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTTTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGGGCCAACGGTTTATCATCATCCGTACGCTCGCTTACCCCCGGGCTGGACAAAAAACGCTTTAACAATTCAGGGAATAAATCGAgttgt                                                                                                            >  1:1070438/1‑239 (MQ=255)
                                                                                         ccTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAAATGTTGTTTTCAACCGCCAAGAGAAACTCAATCTGCCCACAGCGCCAGTGCCAACGGTTTTTCATCATCCGTACGCTCGTTTAACGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAgttgtt                                                                                                           >  2:1048379/1‑237 (MQ=255)
                                                                                            ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTTTTTTCTTCCACCGCCATTAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACCGTTTTTCCTTCTCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAAACGCTTTAACAATTTAGGGAAAAAATAGAattttttccgc                                                                                                      >  1:604140/1‑234 (MQ=255)
                                                                                            ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGGTGTTCCTGc                                                                                                      >  2:801298/1‑239 (MQ=255)
                                                                                              ttttgggtcccttaccaaaaaaaAGAGGCCGAAGACAAAAGACAAAAATTGGTTCGATAACCGCCCTTTATATTTATTTTTAAACCGGGGGGTTAAATTTTTTTCTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGc                                                                                                      <  2:692305/222‑1 (MQ=255)
                                                                                                     aCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTTTTTTCAACCGCCATGAGAAACTCACTCTTCCCACAGCGCCAGTGCCACAGGTTTTTCATCATCCGCACGCTCGTTTACCGCCGGGCTGGGCCAAAACCGGTTTTACCATTCAGGTAATTAATCGAGTTGGTCCTGCGTttctgc                                                                                              >  1:1158122/1‑238 (MQ=255)
                                                                                                     aCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTtgctgc                                                                                              >  2:492121/1‑238 (MQ=255)
                                                                                                           aTCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGTATTTTTTCCTTCAACCGCCATTAGAAACTCACTCTGCCCCCAGCGCCACGTCCAACCGTTTTTTATCAACCGTACGCCCGCTTTCCGCCCGGCTGGACCAAAAACCCTTTTACAAATCAGGTAATAAATCGAGTTGTTCCTCCGTTGCTgtcgccc                                                                                         >  1:502177/1‑238 (MQ=255)
                                                                                                                      gaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTTAATTGTTGACTTTAACCGCCATGAGGAAATTACTCTGCCCACAGGGTCAGTGCCAACGTTTTATCATCATCCGTTCGCTCGTTTACCGCCGGGGTGGACAAAAAATGCTTTTACAATTCATGGACTAAATCGAGTTTTTCCTGCGGTGCTTGCTCCCGTCCATTGata                                                                              >  1:252931/1‑237 (MQ=255)
                                                                                                                       aagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTTTTTTCTTTAAACGCCATGAGAAACTCACTCTGCCCACAACGCCAGTGCCAACGTTTTATTATCATCCGCAAGCCCGTTTACCCCCGGGCTGGACACAAAATGCATTATAAATTTAGGGAATAAAACGAGTTGTTTCTGCGTTGCTGCCGCCAGTCCAtttcaaa                                                                             >  1:808528/1‑234 (MQ=255)
                                                                                                                        agCCGACGAGTAAAAAAAAAATTTAGTCCTTTAACCACCCTTTATTTTTTTTTTTTAATCGGGCGTTTAATTTTTTTCTTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGaaaaa                                                                            <  2:604140/239‑1 (MQ=255)
                                                                                                                               acgataaaagaaaaaatTTAGTTCCCTAACCACCCGTTATATTTTTTTTTAAAACGGGCGGTTAATTTGTTGTTTACAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACggg                                                                        <  2:808528/225‑1 (MQ=255)
                                                                                                                                ggcaagaaaacacaaaTTAGCTCTCTCATTCCCCTTTATATTTTTGTTGTAACTGGCGGTTTAAGTTGTTGTCTACCACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCACCCGTTCGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACgggg                                                                       <  1:895467/225‑1 (MQ=255)
                                                                                                                                     tAAAAGAAAATTTTGTTCGTTATCATCCGTTTTTTTTTATTGTTCAGACGGGCGTTTGATTTGTTGTCTCCACCCGCCATGAGAAACTCACGCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGTCAGTCCATTGAAAAACGGGGTaaaga                                                                 <  1:401509/239‑1 (MQ=255)
                                                                                                                                              aaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATTAGAAACTCACTCTGCCCACAGCGCCAGTGCCAAAGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCCGGCCATTTAAAAAAGGGGTAAAGAAAGGca                                                           >  2:858271/1‑236 (MQ=255)
                                                                                                                                                   gTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTTTTGTTTTCCACCCCCATGAGAAACTCACTCTTCCCCCAACCCCCGTGCCCACGTTTTTTCCTCATCCGTACGCTCGTTTTCCCCCCGGCCGGACAAAAAATTCCTTTACAATTTAGGTAATTAAACGAGGTGTTCCTTCGTTGCTTCCGCCCATCCCTTTAAAAAAAGGGGAAAGAAAAGCACATgaggg                                                   >  1:1073900/1‑238 (MQ=255)
                                                                                                                                                    ttCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTTCACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCACCGTTTTATCATCATCCGTACTCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCCAGTTGTTTCTGCGTTTCTGCCGCCCGTCCCTTGGAAAAAGGGGGTAAGACAGGCACCTgagggg                                                  >  1:863741/1‑237 (MQ=255)
                                                                                                                                                    tattcctctaacccccttttATTTTTTTTGTTAAAGGTGGGGTTTGATTGTTTTTTTCCAACCGCCATGAGAACCTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgag                                                     <  1:801298/221‑1 (MQ=255)
                                                                                                                                                        cctaaataGCCTTTTTTTTTTTTGTTTAAACCGGGGGTTTAATTTTTTTCTCCAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGaa                                                <  1:343433/232‑1 (MQ=255)
                                                                                                                                                             aaccagccttattattttttGTTAAACTTGGTGTTTAATTTGTTTCCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTAt                                            <  2:805712/227‑1 (MQ=255)
                                                                                                                                                                gCCTTTATATTTTTTGTTAAAACTGGGGGTTGAATTGTTGTCTACAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGc                                       <  2:1113816/239‑1 (MQ=255)
                                                                                                                                                                  tcttttttattttttGTTAAACCTGGTGTGTGATTTTTTTTTTACACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGc                                       <  2:140672/232‑1 (MQ=255)
                                                                                                                                                                   cctttATATTTTTTTTTTAAAGTGGCGGTTAATTTTTTGTCTCAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCa                                      <  2:944003/236‑1 (MQ=255)
                                                                                                                                                                           tttttttGTTTAACGTGGGGTTTGATTTGTTTTCTCAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTaa                               <  2:863741/238‑1 (MQ=255)
                                                                                                                                                                              tattGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAAATCAATCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCCTACGCCCGTTTACCGCCGGGCTGGGCAAAAAATGCTTTAACAATTTAGGTAATAAATCGAGGTTTTCCTGCGTTGCTGCCGCCCGTCCATTGAAAAACGGGGTAAAGAAAAGCACATGAGGGGAATTATCCAGCATATTTaaag                             >  1:514476/1‑236 (MQ=255)
                                                                                                                                                                               attGTTTAAACTGGCGGTTGAATTGTTGTCTTTAACCGCCATGAGAAACTCACTCTGCCCCCAGCGCCAGTGCCAACGTTTTATCATCATTCGTAAGCTCGTTTACCCCCGGGCTGGACAAAAACTGCTTTAACAATTTAGGAAATAAATAGAGTTGTTCCCGCGTTGCTGCCGCCAGTTCATTCAAAAAAGGGGTAAAGAAAGGCACATGAGGAGGACTATTCCGCATCTTTAACGc                            >  2:60681/1‑238 (MQ=255)
                                                                                                                                                                                     tAAACTGGCGGTTGAAATGTTGTCTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTTAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAAAGCCAGTc                       >  2:442125/1‑237 (MQ=255)
                                                                                                                                                                                         cggcgggTTAAATTTTTCTCTCCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCAGCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGa                 <  2:1158122/234‑1 (MQ=255)
                                                                                                                                                                                          ggggtgTTTAATTTTTTTCTTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGa                 <  2:302663/234‑1 (MQ=255)
                                                                                                                                                                                             gtggtgaattttttgtCTTAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATa               <  2:197106/226‑1 (MQ=255)
                                                                                                                                                                                             cGGGTGAATTTTTGTCTTCAAACGCCATGAGAAACTCACTCTTCCCACAGCGCCAGTGCCCACGTTTTATCATCATCCCTACGCTCGCTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTTCTGCGTTGCTGCCGCCCGTCCCTTGAAAAACGGGGTGAAACAAAGCACATAGGGGGAATTCTCCAGCATATTTAACGCCAATTGCTgggaacc             >  1:470340/1‑234 (MQ=255)
                                                                                                                                                                                                         ttgtCTACACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAtg  <  2:514476/239‑1 (MQ=255)
                                                                                                                                                                                                           gtCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATCCCATCCGTAAAt   >  1:406874/1‑236 (MQ=255)
                                                                                                                                                                                                           |                                                                                                                                                                                                                                                
TGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATG  >  NC_000913/2151496‑2151925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: