Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,367,366 G→T 20.5% D100Y (GAT→TAT)  arnC → undecaprenyl phosphate‑L‑Ara4FN transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,367,3660GT20.5% 137.1 / 8.3 65D100Y (GAT→TAT) arnCundecaprenyl phosphate‑L‑Ara4FN transferase
Reads supporting (aligned to +/- strand):  ref base G (12/39);  new base T (12/2);  total (24/41)
Fisher's exact test for biased strand distribution p-value = 3.81e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

AAGCTTACCGGAATTAATCAGGCGCACCACCACAGCCTGTGAATCGTTGGGGAAAGAGTATGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGA  >  NC_000913/2367131‑2367604
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    
aaGCTTACCGGAATAAACCGGGCGCACACCCACAGCCTGGGAATCGTTGGGGAAAGAGTCTGAGAGCCTGCTGATTGATGACGCCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATg                                                                                                                                                                                                                                                   <  1:1158672/239‑1 (MQ=255)
        cGGAATTAATCAGGCGCACCACCACAGCCTGTGAATCGTTGGGGAAAGAGTATGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTGTGACACGTTACTGGAGACTTTATTATTACACTTGATGCCggtgt                                                                                                                                                                                                                                            >  1:845536/1‑234 (MQ=255)
                  cAGGCGCACCACCACAGCCTGTGAATCGTTGGGGAAAGAGGAGGAGACCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGc                                                                                                                                                                                                                                <  1:533077/239‑1 (MQ=255)
                      cgcACCGCCACAGCCTGTGAATCGATGGGGAAAGAGTAGGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGcc                                                                                                                                                                                                                               <  1:672769/236‑1 (MQ=255)
                             gtataggaaagagttCGTGGGGAAAGGAGTATGAGTTCCGGCTGATGGAGGACGGCAGGGGCGATAATTCCGCGAATATGCAGGTCGAAGCCCACCAGGGGGAAAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCAATTATGGCGGGATTCAGTCACGTTCCTGGCGACTTAATTATTCCCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATc                                                                                                                                                                                                                    <  1:530158/225‑1 (MQ=255)
                                      gtgAATCGTTGGGGAAAGAGTATGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCtg                                                                                                                                                                                                          <  2:1010417/239‑1 (MQ=255)
                                        gAATCGTTGGGGAAAGAGTATGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTTAGTCACCGTTTTTGCGGCTTAATTATTTTCCTTTATGCCCCTCTCCAGAATTCCCCCGAAAAAATCCCCCCCCcggt                                                                                                                                                                                                        >  2:444266/1‑239 (MQ=255)
                                            cGTTGGGGAAAGAGTATGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCa                                                                                                                                                                                                    <  2:75532/239‑1 (MQ=255)
                                                     aaGAGTATGAGTCCCTGCTGTTTGTTGCCGCAAGTAGCGATAATCCCGCCCATAGGCTGGTCGAAGCCTCACAGGGGGAGACCAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGCCACGTTACTGGCGACTTAATTATTCCCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGAt                                                                                                                                                                                           <  1:47417/239‑1 (MQ=255)
                                                     aaGAGTATGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTTAGTCACGTTACTGGCGACTTAATTTTTTCCCCTGATGCCGTTCTCCAAAATCCCCCAGAAAAAATTCCCCCCCcgggctgtttcttcta                                                                                                                                                                                            >  2:530158/1‑225 (MQ=255)
                                                        ggTATGAGAGCCGGCTTATTGACGCCGGCAGTTAGCAAAATTCCGGCGCATATGTTGTCCAAACCCCCAAAGGGGAAAAACAGCCATTTTGTTTCTATTTTGTTAAACCGAAATTCCGGGCAACATTCAGGGATTTGGGGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATg                                                                                                                                                                                          <  1:11831/236‑1 (MQ=255)
                                                        agTATGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTTATTCACGTTACTGGGGACCTTATTATTATCCTTTGTGCCGCTCCTCCCAATCCGCCCAAAGAAAACCCCCCCCCGGTGGCTAAAAcccctgga                                                                                                                                                                                        >  1:1139454/1‑233 (MQ=255)
                                                               tacccgcttgattaatGCCCGCAGGTGAGATATTCCCGCGAATATCCTGTGCTAAGCCTCTAAAGCGGAGACCAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGAAACATTCAGCGATTATGGGGGGATTCAGTAACGTTACTGGCGACTTAATTATTACCCTGGATGCCGAGCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacg                                                                                                                                                                                 <  1:43185/231‑1 (MQ=255)
                                                                 tcttgctgATTGAGACCGGCAGTGGCGATAATCCCGCGCATATGCTGGTCAAAGCCCCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacgac                                                                                                                                                                               <  1:439051/236‑1 (MQ=255)
                                                                 tCCTGCTGATTGAGGACGGCAGTAGCAAAAATCCCGCGAATATCCTGGTGGAAGCCCCACAAGCGGAGACCAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacgac                                                                                                                                                                               <  1:306655/239‑1 (MQ=255)
                                                                 tCCTGCGTATGGAGGACGGCGGTGGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACGAGCGGAGAACAGCCATATTGTGTTTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTAACGTTACTGGCGACTTAATTATTGCCCTTGATGCCGATCTCCAGAAACCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacgac                                                                                                                                                                               <  1:589613/239‑1 (MQ=255)
                                                                 gccTGCTGATTGATGACGGCAGTAGCAATAATTCCGCGCATATGCTGGTCAAAGCCCCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacgac                                                                                                                                                                               <  1:778735/238‑1 (MQ=255)
                                                                  ccTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGTTTCGTTCCCGTTCTGGGGGCCTTATTTTTTACCCTTGTGCCGGTCCTCCGAAATCCCCCGCAGAAAATCCCCCCCCCGGGGGCaaaaccgcagaagggttagac                                                                                                                                                                                >  2:11831/1‑220 (MQ=255)
                                                                  ccTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGCTTTAGTCACGTTATTGGCGGCTTAATTATTAACACTTATGCCGCTCTCCAGAAACAGCCCAAAGAAAAACCCCCCCCGTGGGGAAAAACCCACGAAAGTTacgccg                                                                                                                                                                              >  2:1140137/1‑239 (MQ=255)
                                                                    tgctgATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGTGTTTAATCACGTTACTTGCGACCTAATTTTTTCCCTTTTTGCCGGTCTCCCGAAACCACCCGAAAAAAAACCCCCCCCTGGGGGCAAAACCGATGAAAGGTacgacggg                                                                                                                                                                            >  1:918768/1‑237 (MQ=255)
                                                                    tgctgATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTTATTCACGTTACTGGCGGCTTTATTATTATCCTTTTTTCCGATCTTCCGAATCCCCCCAAAGAAATCCCCCCCCTGGGGGCAAAAAACGATGaaaggtaacaagtg                                                                                                                                                                            >  2:538376/1‑227 (MQ=255)
                                                                    tgctgATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGGTACTGGCGACTTCATTATTACCCCTGATTGCCACCTCCAGGATCCCCCACAAGAAATCCCCCCCCTGGTGGCGAAAGCCGACGAAGGGTacaacggc                                                                                                                                                                            >  2:255111/1‑237 (MQ=255)
                                                                       tgattgatGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGt                                                                                                                                                                          <  2:82400/238‑1 (MQ=255)
                                                                         attgatGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTTAGTCACGTTACTGGCGACTTAATTTTTAACCTTTTTGCCGATCTCCCGAATCCGCCAGAAGAAAACCCCCGCCTGGTGGCAAAAGCCGATGAAGGGTacccactgggagg                                                                                                                                                                       >  2:59281/1‑228 (MQ=255)
                                                                          ttgatGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTCCCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAgg                                                                                                                                                                       <  2:94127/238‑1 (MQ=255)
                                                                           tgatgaCGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTTAGTCACGTTTCTGGCGGCTTTATTTTTAACCTTTATGGCGATCTTCAGAAAACGCCCGAAGAAATCCACCCCCTGGTGGCGAAAAACGATGAAGGTTACGACGGGGTAgggg                                                                                                                                                                     >  2:399787/1‑238 (MQ=255)
                                                                           tgatgaCGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGa                                                                                                                                                                     <  2:512218/239‑1 (MQ=255)
                                                                           tgaggaCGGCAGTGGCGATAATTCCGCGCATATGCTGGTCGAGGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGa                                                                                                                                                                     <  1:618158/239‑1 (MQ=255)
                                                                                  ggCAGTAGCGATAATCCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACg                                                                                                                                                              <  2:641864/239‑1 (MQ=255)
                                                                                                                               aaGGGGAGAACAAGCATTTTTGTGTCTATTTGGCTTAACCCAAATTACGTGCAAGATTCAGCGATTATGGCGGGTTTCAGCCACGTTGCTGGCAACTTAATTTTTCCCCTTGATGCCGTACTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGaa                                                                                                                  <  1:930752/239‑1 (MQ=255)
                                                                                                                                       accAGCAATTTTGTTTCTATTTTGCTTAACCGCAATTCCGGGAAACATTCAGCGATTAGGGGGGGATTCAGCCACGTTACTGGCGATTTAATTATTCCCCTGGATGCCAATCTCCAGAATCCGCCAGAAGAAACCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTaa                                                                                                         <  1:59281/237‑1 (MQ=255)
                                                                                                                                        accatctattttGTGTCTATTTTGCTTAACCGAAATTCCGGGCAACATTCAGCGATTATGGGGGGATTCAGTCACGTTACTGGCGACTTAATTATTCCCCTTGATGCCGATCCCCAGAATCCGCCAGAAGAAACCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTaa                                                                                                         <  2:918768/229‑1 (MQ=255)
                                                                                                                                        accagccttttgtggtCTATTTTGTTAAACCGAAATTACGGGAAACATTAAGCGTTTATGGGGGATTTCGTGCCCGTACCGGGCAATTTAATTATTCCCTTTGTGGCGGATCTCCAGAACCCCCGAGAAGAAACCCCCGGCCGGTGGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTaa                                                                                                         <  1:903107/225‑1 (MQ=255)
                                                                                                                                             cctttttGGGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGCCCCGTTACTGGCAACTTAATAATTCCCCTTGATGCCAATCCCAAGAACCCGCCAAAAGAAACCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGc                                                                                                    <  1:508671/233‑1 (MQ=255)
                                                                                                                                                ttttGTGTGTTTTTTGCTTAACCCAAATACCGGGCACCATTCAGCGATTAGGGCGGAATTCAGTCACGTTCCTGGCGACTTAATTATCCCCCTGGAGGCCGATCCCCAGAATCCCCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTGGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTa                                                                                                 <  1:399787/237‑1 (MQ=255)
                                                                                                                                                  ttGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTTAGTCACGGTATAAGGGAATCAATTATTTATCTTTAAGCCGAGCTCCACAAACCCCCAGAAGGAATCACCCCCCCCGGGGGTAAAAACGAAGAAGGGTAAGCAGGGGGATGGACTGCACGCCAGAAACGCCAGGAAAGCGGGTTTCGTAACAACGCATTTGAAgaggataatcggcgttc                                                                                                 >  1:185516/1‑227 (MQ=255)
                                                                                                                                                       tCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTCAGTCACGTTACTTGCGGCTTTATTATTACCCTTTATGCCGATCTCCAGAATCCCCCAGAAGAAATCCCCCCCCTGGTGGCAAAAACCCATGAAAGTTACGACGTGGGAGGGACTGTACGCCCGAACCGCCCGGACAGCCGGTTTCGTAAAACCCCTTCGAAGATGATTAACCGGCTTATTCatc                                                                                           >  2:589919/1‑236 (MQ=255)
                                                                                                                                                       tCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGGTTAGTCACGGTACCGGGGGCTTTATTTTTACTCTTTTTGCCGCTCCCCCGAATCCCCCACCAAAAAACCCCCCCCCGGTGGGAAAAACCGATCAAGGTTATGACGAGGGAGGGGGTGGACGCCAGAACACCCACGCCAACTCGTTTTGTTAAAACCCCTCGTAgaaggttcacacgcctcttctac                                                                                           >  1:1149930/1‑217 (MQ=255)
                                                                                                                                                       tCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTTAATCACCATTCTGGCGACTTAATTATTTCCCCTTATGCCGATGTCCAAAAACCCCCACAAAAAAACCCCCCCCTCGTGGCTAAAACCCAAGAAAGGTACCCCGTGGGAGGGGCAGTACGCCCGAAACACCAGGACAGGTGGGTTGTGAAAAAAGCATCGGAGAGGAATAAcacggttaattaga                                                                                           >  1:36779/1‑225 (MQ=255)
                                                                                                                                                       tCTATTTTCCTAACCCCCAATCGCGGCAACAATTAGCGGTTTTGGGAGGAATTCAGTCCCGTTTGGGTCGCCTTACTTCTTACCCTGTATCCCGTCCCCAAAAACCCCCCAGAAGAAACCCCCCCCCTGTTGGCAAAAGCAGATGAAGTTAACGACGTGTTAGGACCTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAgcg                                                                                         <  1:298486/239‑1 (MQ=255)
                                                                                                                                                       gtgatttgacTTACCCGCAATAACGGGCAAGATACAGCATTTAGGGCGGGATTCAGTCACGTTACGGACGACTAATTGTTTCCCCTTGAGGCCAAGCCCCAGATTCCGCAAGAAGAAACCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTGCGACGTGGAAGGGACTGTACGCCAGATCCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAgcg                                                                                         <  1:255111/230‑1 (MQ=255)
                                                                                                                                                                 ttAAACGAAAATACGGGCAACATACATCGATTATGCGGGGATTCGTTCCCTTTGCTGGTGCTTTAATTTTTCCCGTGGAGTCCGATCTCCAGAACCCGCGAAAAAAAAGCCCCCGCCTGGTGGCAAAAGCCGATGAAGTTTACGATGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGc                                                                               <  1:444266/239‑1 (MQ=255)
                                                                                                                                                                   aaCCGCAATTACGGGCAACATTCAGCGATTATGGCGGTGTTTAGTCCCGTTACTTGCGACCTAATTATTATCCTTTTTGCCGATCTCCCGAATCCGCCAGCAGAAAACCCCCCCCTGGTGGCCCAAGCCGGCGAAAGTTACGACGTGGGTGGGACGGTTCGGCAGAAACCCCCAGGCCGCCGGGTTCCTTAAACCCCTCCCGAGAAGAGTAAACCGCTTATTCATGCGcacaacggcac                                                                              >  1:333737/1‑234 (MQ=255)
                                                                                                                                                                       gCAATTCCGGGCACATTTCAGGGATTATGGCGGGATCCAGCAACGTTCCTGGCAACTAAATTATTCCCCTTGAGGCCGATCCCCAAAACCCGCCAGAAAAATCCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTAGGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCg                                                                         <  1:522021/239‑1 (MQ=255)
                                                                                                                                                                           ttACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATgg                                                                     <  2:583008/239‑1 (MQ=255)
                                                                                                                                                                                 caacaagtCAGCGTTTAGGGGGGGATCCAGTCAGTTTACTGGCGACTTAATTATTCCCCTGGAGGCCGATCCCCAGAACCCCCCAGAAAAAACCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGAt                                                                <  1:538376/231‑1 (MQ=255)
                                                                                                                                                                                  aaaCAGTCAGGGATTAGGGGGGAAGCCAGCCGCGTTGTGGGCGACATAATTATTCCCCTGGTTGCCGAGCTCAGGAACCCGCCAGAAGAAATCCCCCGCCTGGTGGAAAAAGCCGAGGAAGGTTACGACGTGGTAGGGCCGGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTa                                                              <  1:987187/238‑1 (MQ=255)
                                                                                                                                                                                    aaaTTCAGCGTTTATGGGGGGTTTCAGCAACGTTCCTGGGGATTAATTTTTTCCCCTGGAGGCCGATCCCAAGAATCCCCAGGAAGAATCCCCCCGCCGGGGGGCAAAACCCGATGAGGGTTACGAGGTGGTAGGGACTGTCCGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACg                                                            <  1:589919/237‑1 (MQ=255)
                                                                                                                                                                                             ggttAGGGCGGAATTCAGCCGTGTTCGTGCGGCTTAAATTATACCCTTAGAGGCCGATCTCCAAAACCCTCCAAAAGAAATCCCCCGCCGGGTGGAAAAAGCCGATGAAGGTTACGACGTGGTGGGGCCTGTACGCCAGACCCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTa                                                      <  1:1206403/234‑1 (MQ=255)
                                                                                                                                                                                                tATGGCGGGATTCAGTCACGTTCCTGGCGACTTAATTTTTCCCCTTGATCCCGATCTCCAGAAACCGCCAGAAGAAACCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTGGGGATTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTAtgctgc                                                <  2:928314/239‑1 (MQ=255)
                                                                                                                                                                                                          aTTCAGTCACGTGACGGGAGATTTAATTATGCCCCTGTGTGCAGATGTCCAGAACCCGCCAGAAGAAATCCCCCGCCTGGGGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTAt                                        <  1:1070730/238‑1 (MQ=255)
                                                                                                                                                                                                                    cGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAACCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCata                              <  2:1139454/239‑1 (MQ=255)
                                                                                                                                                                                                                     gTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTa                                                              >  1:720222/1‑206 (MQ=255)
                                                                                                                                                                                                                     gTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTa                                                              <  2:720222/206‑1 (MQ=255)
                                                                                                                                                                                                                         cTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTc                         >  2:297502/1‑239 (MQ=255)
                                                                                                                                                                                                                          tGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTcg                        >  1:460712/1‑239 (MQ=255)
                                                                                                                                                                                                                           ggCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAGGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGGAGGGACGGTACGCCAGAACCGCCAGGACAGGTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCTCACCACTGGCAAAGCGATGGGTGATTACGGTGGTATTCTTCGCGCCTCTCGCCGTCATATTGTcgt                       >  1:236727/1‑238 (MQ=255)
                                                                                                                                                                                                                              gACTTAATTATTCCCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTcgatg                     <  2:845536/238‑1 (MQ=255)
                                                                                                                                                                                                                               aCTTATTTATTCCCCTGGATGCCGATCCCCAAAAGCGGCAGAAAGAAACCCCCCGCCTGGTGGCAAAAGCCGATGAAGTTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTcgatgcg                   <  1:297560/239‑1 (MQ=255)
                                                                                                                                                                                                                               aCTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCCTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTcgatgcg                   >  2:872031/1‑239 (MQ=255)
                                                                                                                                                                                                                                 ttAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTcgatgcgat                 >  2:475296/1‑239 (MQ=255)
                                                                                                                                                                                                                                       attcCCCTTGATGCCGATCTCCAAAATCCCCGAGAAGAAATCCCCCGCCTGGTGCCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACGGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCa           <  2:185516/239‑1 (MQ=255)
                                                                                                                                                                                                                                         tccccGTGATGCCAATCGCCGAAACCCGCCAAAAAAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACt         <  2:518576/237‑1 (MQ=255)
                                                                                                                                                                                                                                          aCCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACt         >  2:158310/1‑238 (MQ=255)
                                                                                                                                                                                                                                             cTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCa     >  2:896004/1‑239 (MQ=255)
                                                                                                                                                                                                                                                 gATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGa  >  2:651384/1‑239 (MQ=255)
                                                                                                                                                                                                                                                 gATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGa  >  2:651234/1‑239 (MQ=255)
                                                                                                                                                                                                                                                 gATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGa  >  1:1038544/1‑239 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    
AAGCTTACCGGAATTAATCAGGCGCACCACCACAGCCTGTGAATCGTTGGGGAAAGAGTATGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGA  >  NC_000913/2367131‑2367604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: