Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,367,366	G→T	20.5%	D100Y (GAT→TAT)	arnC →	undecaprenyl phosphate‑L‑Ara4FN transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,367,366	0	G	T	20.5%	137.1 / 8.3	65	D100Y (GAT→TAT)	arnC	undecaprenyl phosphate‑L‑Ara4FN transferase
Reads supporting (aligned to +/- strand):  ref base G (12/39);  new base T (12/2);  total (24/41)
Fisher's exact test for biased strand distribution p-value = 3.81e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

AAGCTTACCGGAATTAATCAGGCGCACCACCACAGCCTGTGAATCGTTGGGGAAAGAGTATGAGATCCTGCTGATTGATGACGGCAG‑TAGCGATAATTCC‑GCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGATTCAGT‑CACGTTACTGGCGACTTAATTATTACCCTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGA  >  NC_000913/2367131‑2367604                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     aaGCTTACCGGAATAAACCGGGCGCACACCCACAGCCTGGGAATCGTTGGGGAAAGAGTCTGAGAGCCTGCTGATTGATGACGCCAG‑TAGCGATAATTCC‑GCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGATTCAGT‑CACGTTACTGGCGACTTAATTATTACCCTT‑GATg                                                                                                                                                                                                                                                   <  1:1158672/239‑1 (MQ=255)         cGGAATTAATCAGGCGCACCACCACAGCCTGTGAATCGTTGGGGAAAGAGTATGAGATCCTGCTGATTGATGACGGCAG‑TAGCGATAATTCC‑GCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGGTTGTGA‑CACGTTACTGGAGACTTTATTATTACACTT‑GATGCCggtgt                                                                                                                                                                                                                                            >  1:845536/1‑234 (MQ=255)                   cAGGCGCACCACCACAGCCTGTGAATCGTTGGGGAAAGAGGAGGAGACCCTGCTGATTGATGACGGCAG‑TAGCGATAATTCC‑GCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGATTCAGT‑CACGTTACTGGCGACTTAATTATTACCCTT‑GATGCCGAT‑CTCCAGAATCCGc                                                                                                                                                                                                                                <  1:533077/239‑1 (MQ=255)                       cgcACCGCCACAGCCTGTGAATCGATGGGGAAAGAGTAGGAGATCCTGCTGATTGATGACGGCAG‑TAGCGATAATTCC‑GCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGATTCAGT‑CACGTTACTGGCGACTTAATTATTACCCTT‑GATGCCGAT‑CTCCAGAATCCGcc                                                                                                                                                                                                                               <  1:672769/236‑1 (MQ=255)                              gtataggaaagagttCGTGGGGAAAGGAGTATGAGTTCCGGCTGATGGAGGACGGCAG‑GGGCGATAATTCC‑GCGAATATGCAGGTCGAAGCCCACCAGGGGGAAAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCAATTAT‑GGCGGGATTCAGT‑CACGTTCCTGGCGACTTAATTATTCCCCTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATc                                                                                                                                                                                                                    <  1:530158/225‑1 (MQ=255)                                       gtgAATCGTTGGGGAAAGAGTATGAGATCCTGCTGATTGATGACGGCAG‑TAGCGATAATTCC‑GCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGATTCAGT‑CACGTTACTGGCGACTTAATTATTACCCTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCtg                                                                                                                                                                                                          <  2:1010417/239‑1 (MQ=255)                                         gAATCGTTGGGGAAAGAGTATGAGATCCTGCTGATTGATGACGGCAG‑TAGCGATAATTCC‑GCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGATTTAGT‑CACCGTTTTTGCGGCTTAATTATTTTCCTT‑TATGCCCCT‑CTCCAGAATTCCCCCG‑AAAAAATC‑CCCCCCCcggt                                                                                                                                                                                                        >  2:444266/1‑239 (MQ=255)                                             cGTTGGGGAAAGAGTATGAGATCCTGCTGATTGATGACGGCAG‑TAGCGATAATTCC‑GCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGATTCAGT‑CACGTTACTGGCGACTTAATTATTACCCTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCa                                                                                                                                                                                                    <  2:75532/239‑1 (MQ=255)                                                      aaGAGTATGAGTCCCTGCTGTTTGTTGCCGCAAG‑TAGCGATAATCCC‑GCCCATAGGCTGGTCGAAGCCTCACAGGGGGAGACC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGATTCAGC‑CACGTTACTGGCGACTTAATTATTCCCCTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGAt                                                                                                                                                                                           <  1:47417/239‑1 (MQ=255)                                                      aaGAGTATGAGATCCTGCTGATTGATGACGGCAG‑TAGCGATAATTCC‑GCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGGTTTAGT‑CACGTTACTGGCGACTTAATTTTTTCCCCT‑GATGCCGTT‑CTCCAAAATCCCCCAG‑AAAAAATT‑CCCCCCCcgggctgtttcttcta                                                                                                                                                                                            >  2:530158/1‑225 (MQ=255)                                                         ggTATGAGAGCCGGCTTATTGACGCCGGCAGTTAGC‑AAAATTCCGGCGCATATGTTGTCCAAACCCCCAAAGGGGAAAAAC‑AGCCATTTTGTTTCTATTTTGTTAAACCGAAATTCCGGGCAACATTCAGGGATT‑T‑GGGGGGATTCAGT‑CACGTTACTGGCGACTTAATTATTACCCTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATg                                                                                                                                                                                          <  1:11831/236‑1 (MQ=255)                                                         agTATGAGATCCTGCTGATTGATGACGGCAG‑TAGCGATAATTCC‑GCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGATTTATT‑CACGTTACTGGGGACCTTATTATTATCCTT‑TGTGCCGCT‑CCTCCCAATCCGCCCA‑AAGAAAAC‑CCCCCCCCGGTGGCTAAAAcccctgga                                                                                                                                                                                        >  1:1139454/1‑233 (MQ=255)                                                                tacccgcttgattaatGCCCGCAG‑GTGAGATATTCCC‑GCGAATATCCTGTGCTAAGCCTCTAAAGCGGAGACC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGAAACATTCAGCGATTAT‑GGGGGGATTCAGT‑AACGTTACTGGCGACTTAATTATTACCCTG‑GATGCCGAG‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacg                                                                                                                                                                                 <  1:43185/231‑1 (MQ=255)                                                                  tcttgctgATTGAGACCGGCAG‑TGGCGATAATCCC‑GCGCATATGCTGGTCAAAGCCCCACAAGCGGAGAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGATTCAGT‑CACGTTACTGGCGACTTAATTATTACCCTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacgac                                                                                                                                                                               <  1:439051/236‑1 (MQ=255)                                                                  tCCTGCTGATTGAGGACGGCAG‑TAGCAAAAATCCC‑GCGAATATCCTGGTGGAAGCCCCACAAGCGGAGACC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGATTCAGT‑CACGTTACTGGCGACTTAATTATTACCCTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacgac                                                                                                                                                                               <  1:306655/239‑1 (MQ=255)                                                                  tCCTGCGTATGGAGGACGGCGG‑TGGCGATAATTCC‑GCGCATATGCTGGTCGAAGCCTCACGAGCGGAGAAC‑AGCCATATTGTGTTTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGATTCAGT‑AACGTTACTGGCGACTTAATTATTGCCCTT‑GATGCCGAT‑CTCCAGAAACCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacgac                                                                                                                                                                               <  1:589613/239‑1 (MQ=255)                                                                  gccTGCTGATTGATGACGGCAG‑TAGCAATAATTCC‑GCGCATATGCTGGTCAAAGCCCCACAAGCGGAGAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGATTCAGT‑CACGTTACTGGCGACTTAATTATTACCCTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacgac                                                                                                                                                                               <  1:778735/238‑1 (MQ=255)                                                                   ccTGCTGATTGATGACGGCAG‑TAGCGATAATTCC‑GCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGTTTCGTT‑CCCGTTCTGGGGGCCTTATTTTTTACCCTT‑G‑TGCCGGTCCTCCGAAATCCCCCGC‑AGAAAATC‑CCCCCCCCGGGGGCaaaaccgcagaagggttagac                                                                                                                                                                                >  2:11831/1‑220 (MQ=255)                                                                   ccTGCTGATTGATGACGGCAG‑TAGCGATAATTCC‑GCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGCTTTAGT‑CACGTTATTGGCGGCTTAATTATTAACACT‑TATGCCGCT‑CTCCAGAAACAGCCCA‑AAGAAAAA‑CCCCCCCCGTGGGGAAAAACCCACGAAAGTTacgccg                                                                                                                                                                              >  2:1140137/1‑239 (MQ=255)                                                                     tgctgATTGATGACGGCAG‑TAGCGATAATTCC‑GCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGTGTTTAAT‑CACGTTACTTGCGACCTAATTTTTTCCCTT‑TTTGCCGGT‑CTCCCGAAACCACCCGAAAAAAAAC‑CCCCCCCTGGGGGCAAAA‑CCGATGAAAGGTacgacggg                                                                                                                                                                            >  1:918768/1‑237 (MQ=255)                                                                     tgctgATTGATGACGGCAG‑TAGCGATAATTCC‑GCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGATTTATT‑CACGTTACTGGCGGCTTTATTATTATCCTT‑TTTTCCGAT‑CTTCCGAATCCCCCCA‑AAGAAATC‑CCCCCCCTGGGGGCAAAAAACGATGaaaggtaacaagtg                                                                                                                                                                            >  2:538376/1‑227 (MQ=255)                                                                     tgctgATTGATGACGGCAG‑TAGCGATAATTCC‑GCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGATTCAGT‑CACGGTACTGGCGACTTCATTATTACCCCT‑GATTGCCAC‑CTCCAGGATCCCCCAC‑AAGAAATC‑CCCCCCCTGGTGGCGAAAGCCGACGAAGGGTacaacggc                                                                                                                                                                            >  2:255111/1‑237 (MQ=255)                                                                        tgattgatGACGGCAG‑TAGCGATAATTCC‑GCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGATTCAGT‑CACGTTACTGGCGACTTAATTATTACCCTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGt                                                                                                                                                                          <  2:82400/238‑1 (MQ=255)                                                                          attgatGACGGCAG‑TAGCGATAATTCC‑GCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGATTTAGT‑CACGTTACTGGCGACTTAATTTTTAACCTT‑TTTGCCGAT‑CTCCCGAATCCGCCAG‑AAGAAAAC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGGTacccactgggagg                                                                                                                                                                       >  2:59281/1‑228 (MQ=255)                                                                           ttgatGACGGCAG‑TAGCGATAATTCC‑GCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGATTCAGT‑CACGTTACTGGCGACTTAATTATTCCCCTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAgg                                                                                                                                                                       <  2:94127/238‑1 (MQ=255)                                                                            tgatgaCGGCAG‑TAGCGATAATTCC‑GCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGATTTAGT‑CACGTTTCTGGCGGCTTTATTTTTAACCTT‑TATGGCGAT‑CTTCAGAAAACGCCCG‑AAGAAATC‑CACCCCCTGGTGGCGAAAAACGATGAAGGTTACGACGGGGTAgggg                                                                                                                                                                     >  2:399787/1‑238 (MQ=255)                                                                            tgatgaCGGCAG‑TAGCGATAATTCC‑GCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGATTCAGT‑CACGTTACTGGCGACTTAATTATTACCCTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGa                                                                                                                                                                     <  2:512218/239‑1 (MQ=255)                                                                            tgaggaCGGCAG‑TGGCGATAATTCC‑GCGCATATGCTGGTCGAGGCCTCACAAGCGGAGAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGATTCAGT‑CACGTTACTGGCGACTTAATTATTACCCTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGa                                                                                                                                                                     <  1:618158/239‑1 (MQ=255)                                                                                   ggCAG‑TAGCGATAATCCC‑GCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGATTCAGT‑CACGTTACTGGCGACTTAATTATTACCCTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACg                                                                                                                                                              <  2:641864/239‑1 (MQ=255)                                                                                                                                aaGGGGAGAACAAGCATTTTTGTGTCTATTTGGCTTAACCCAAATTACGTGCAAGATTCAGCGATTAT‑GGCGGGTTTCAGC‑CACGTTGCTGGCAACTTAATTTTTCCCCTT‑GATGCCGTA‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGaa                                                                                                                  <  1:930752/239‑1 (MQ=255)                                                                                                                                        acc‑AGCAATTTTGTTTCTATTTTGCTTAACCGCAATTCCGGGAAACATTCAGCGATTAG‑GGGGGGATTCAGC‑CACGTTACTGGCGATTTAATTATTCCCCTG‑GATGCCAAT‑CTCCAGAATCCGCCAG‑AAGAAACC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTaa                                                                                                         <  1:59281/237‑1 (MQ=255)                                                                                                                                         accatctattttGTGTCTATTTTGCTTAACCGAAATTCCGGGCAACATTCAGCGATTAT‑GGGGGGATTCAGT‑CACGTTACTGGCGACTTAATTATTCCCCTT‑GATGCCGAT‑CCCCAGAATCCGCCAG‑AAGAAACC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTaa                                                                                                         <  2:918768/229‑1 (MQ=255)                                                                                                                                         accagccttttgtggtCTATTTTGTTAAACCGAAATTACGGGAAACATTAAGCGTTTAT‑GG‑GGGATTTCGTGCCCGTACCGGGCAATTTAATTATTCCCTTT‑GTGGCGGAT‑CTCCAGAACCCCCGAG‑AAGAAACC‑CCCGGCCGGTGGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTaa                                                                                                         <  1:903107/225‑1 (MQ=255)                                                                                                                                              cctttttGGGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGATTCAGC‑CCCGTTACTGGCAACTTAATAATTCCCCTT‑GATGCCAAT‑CCCAAGAACCCGCCAA‑AAGAAA‑C‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGc                                                                                                    <  1:508671/233‑1 (MQ=255)                                                                                                                                                 ttttGTGTGTTTTTTGCTTAACCCAAATACCGGGCACCATTCAGCGATTAG‑GGCGGAATTCAGT‑CACGTTCCTGGCGACTTAATTATCCCCCTG‑GAGGCCGAT‑CCCCAGAATCCCCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTGGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTa                                                                                                 <  1:399787/237‑1 (MQ=255)                                                                                                                                                   ttGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGGTTTAGT‑CACGGTATAAGGGAATCAATTATTTATCTT‑TAAGCCGAG‑CTCCACAAACCCCCAG‑AAGGAATCACCCCCCCCGGGGGTAAAA‑ACGAAGAAGGGTAAGCAGGGGGATGGACTGCACGCCAG‑AAACGCCAGGAAAGCGGGTTTCGTAACAACGCATTTGAAgaggataatcggcgttc                                                                                                 >  1:185516/1‑227 (MQ=255)                                                                                                                                                        tCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGGTTCAGT‑CACGTTACTTGCGGCTTTATTATTACCCTT‑TATGCCGAT‑CTCCAGAATCCCCCAG‑AAGAAATC‑CCCCCCCTGGTGGCAAAAACCCATGAAAGTTACGACGTGGGAGGGACTGTACGCCCG‑AACCGCCCGGACAGCCGGTTTCGTAAAACCCC‑TTCGAAGATGATTAACCGGCTTATTCatc                                                                                           >  2:589919/1‑236 (MQ=255)                                                                                                                                                        tCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGGGTTAGT‑CACGGTACCGGGGGCTTTATTTTTACTCTT‑TTTGCCGCT‑CCCCCGAATCCCCCAC‑CAAAAAAC‑CCCCCCCCGGTGGGAAAAACCGATCAAGGTTATGACGAGGGAGGGGGTGGACGCCAG‑AACACCCACGCCAACTCGTTTTGTTAAAACCC‑CTCGTAgaaggttcacacgcctcttctac                                                                                           >  1:1149930/1‑217 (MQ=255)                                                                                                                                                        tCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGATTTAAT‑CACCATTCTGGCGACTTAATTATTTCCCCT‑TATGCCGAT‑GTCCAAAAACCCCCAC‑AAAAAAAC‑CCCCCCCTCGTGGCTAAAACCCAAGAAAGGTACCCCGTGGGAGGGGCAGTACGCCCG‑AAACACCAGGACAGGTGGGTTGTGAAAAAAGC‑ATCGGAGAGGAATAAcacggttaattaga                                                                                           >  1:36779/1‑225 (MQ=255)                                                                                                                                                        tCTATTTTCCTAACCCCCAATCGC‑GGCAACAATTAGCGGTTTTGGGAGGAATTCAGT‑CCCGTTTGGGTCGCCTTACTTCTTACCCTG‑TATCCCGTC‑CCCAAAAACCCCCCAG‑AAGAAACC‑CCCCCCCTGTTGGCAAAAGCAGATGAAGTTAACGACGTGTTAGGACCTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑gcg                                                                                         <  1:298486/239‑1 (MQ=255)                                                                                                                                                        gtgatttgacTTACCCGCAATAACGGGCAAGATACAGCATTTAG‑GGCGGGATTCAGT‑CACGTTACGGACGACTAATTGTTTCCCCTT‑GAGGCCAAG‑CCCCAGATTCCGCAAG‑AAGAAACC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTGCGACGTGGAAGGGACTGTACGCCAG‑ATCCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑gcg                                                                                         <  1:255111/230‑1 (MQ=255)                                                                                                                                                                  ttAAACGAAAATACGGGCAACATACATCGATTAT‑GCGGGGATTCGTT‑CCCTTTGCTGGTGCTTTAATTTTTCCCGTG‑GAGTCCGAT‑CTCCAGAACCCGCGAA‑AAAAAAGC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGTTTACGATGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGc                                                                               <  1:444266/239‑1 (MQ=255)                                                                                                                                                                    aaCCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGTGTTTAGT‑CCCGTTACTTGCGACCTAATTATTATCCTT‑TTTGCCGAT‑CTCCCGAATCCGCCAG‑CAGAAAAC‑CCCCCCCTGGTGGCCCAAGCCGGCGAAAGTTACGACGTGGGTGGGACGGTTCGGCAGAAACCCCCAGG‑CCGCCGGGTTCCTTAAACCCC‑TCCCGAGAAGAGTAAACCGCTTATTCATGCGcacaacggcac                                                                              >  1:333737/1‑234 (MQ=255)                                                                                                                                                                        gCAATTCCGGGCACATTTCAGGGATTAT‑GGCGGGATCCAGC‑AACGTTCCTGGCAACTAAATTATTCCCCTT‑GAGGCCGAT‑CCCCAAAACCCGCCAG‑AAAAATCC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTAGGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGCAAAGCg                                                                         <  1:522021/239‑1 (MQ=255)                                                                                                                                                                            ttACGGGCAACATTCAGCGATTAT‑GGCGGGATTCAGT‑CACGTTACTGGCGACTTAATTATTACCCTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGCAAAGCGATgg                                                                     <  2:583008/239‑1 (MQ=255)                                                                                                                                                                                  caacaagtCAGCGTTTAG‑GGGGGGATCCAGT‑CAGTTTACTGGCGACTTAATTATTCCCCTG‑GAGGCCGAT‑CCCCAGAACCCCCCAG‑AAAAAACC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGCAAAGCGATGGGTGAt                                                                <  1:538376/231‑1 (MQ=255)                                                                                                                                                                                   aaaCAGTCAGGGATTAG‑GGGGGAAGCCAGC‑CGCGTTGTGGGCGACATAATTATTCCCCTG‑GTTGCCGAG‑CTCAGGAACCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGAAAAAGCCGAGGAAGGTTACGACGTGGTAGGGCCGGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGCAAAGCGATGGGTGATTa                                                              <  1:987187/238‑1 (MQ=255)                                                                                                                                                                                     aaaTTCAGCGTTTAT‑GGGGGGTTTCAGC‑AACGTTCCTGGGGATTAATTTTTTCCCCTG‑GAGGCCGAT‑CCCAAGAATCCCCAGG‑AAGAATCC‑CCCCGCCGGGGGGCAAAACCCGATGAGGGTTACGAGGTGGTAGGGACTGTCCGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGCAAAGCGATGGGTGATTACg                                                            <  1:589919/237‑1 (MQ=255)                                                                                                                                                                                              ggttAG‑GGCGGAATTCAGC‑CGTGTTCGT‑GCGGCTTAAATTATACCCTTAGAGGCCGAT‑CTCCAAAACCCTCCAA‑AAGAAATC‑CCCCGCCGGGTGGAAAAAGCCGATGAAGGTTACGACGTGGTGGGGCCTGTACGCCAG‑ACCCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTa                                                      <  1:1206403/234‑1 (MQ=255)                                                                                                                                                                                                 tAT‑GGCGGGATTCAGT‑CACGTTCCTGGCGACTTAATTTTTCCCCTT‑GATCCCGAT‑CTCCAGAAACCGCCAG‑AAGAAACC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTGGGGATTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTAtgctgc                                                <  2:928314/239‑1 (MQ=255)                                                                                                                                                                                                           aTTCAGT‑CACGTGACGGGAGATTTAATTATGCCCCTG‑TGTGCAGAT‑GTCCAGAACCCGCCAG‑AAGAAATC‑CCCCGCCTGGGGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTAt                                        <  1:1070730/238‑1 (MQ=255)                                                                                                                                                                                                                     cGTTACTGGCGACTTAATTATTACCCTT‑GATGCCGAT‑CTCCAGAACCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCata                              <  2:1139454/239‑1 (MQ=255)                                                                                                                                                                                                                      gTTACTGGCGACTTAATTATTACCCTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGCAAAGCGATGGGTGATTa                                                              >  1:720222/1‑206 (MQ=255)                                                                                                                                                                                                                      gTTACTGGCGACTTAATTATTACCCTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGCAAAGCGATGGGTGATTa                                                              <  2:720222/206‑1 (MQ=255)                                                                                                                                                                                                                          cTGGCGACTTAATTATTACCCTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTc                         >  2:297502/1‑239 (MQ=255)                                                                                                                                                                                                                           tGGCGACTTAATTATTACCCTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTcg                        >  1:460712/1‑239 (MQ=255)                                                                                                                                                                                                                            ggCGACTTAATTATTACCCTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AGGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGGAGGGACGGTACGCCAG‑AACCGCCAGGACAGGTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCTCACCACTGGCAAAGCGATGGGTGATTACGGTGGTATTCTTCGCGCCTCTCGCCGTCATATTGTcgt                       >  1:236727/1‑238 (MQ=255)                                                                                                                                                                                                                               gACTTAATTATTCCCCTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTcgatg                     <  2:845536/238‑1 (MQ=255)                                                                                                                                                                                                                                aCTTATTTATTCCCCTG‑GATGCCGAT‑CCCCAAAAGCGGCAGA‑AAGAAACC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGTTTACGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTcgatgcg                   <  1:297560/239‑1 (MQ=255)                                                                                                                                                                                                                                aCTTAATTATTACCCTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCCTATTCA‑GCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTcgatgcg                   >  2:872031/1‑239 (MQ=255)                                                                                                                                                                                                                                  ttAATTATTACCCTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTcgatgcgat                 >  2:475296/1‑239 (MQ=255)                                                                                                                                                                                                                                        attcCCCTT‑GATGCCGAT‑CTCCAAAATCCCCGAG‑AAGAAATC‑CCCCGCCTGGTGCCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACGGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCa           <  2:185516/239‑1 (MQ=255)                                                                                                                                                                                                                                          tccccGT‑GATGCCAAT‑CGCCGAAACCCGCCAA‑AAAAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACt         <  2:518576/237‑1 (MQ=255)                                                                                                                                                                                                                                           aCCCTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACt         >  2:158310/1‑238 (MQ=255)                                                                                                                                                                                                                                              cTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCa     >  2:896004/1‑239 (MQ=255)                                                                                                                                                                                                                                                  gATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGa  >  2:651384/1‑239 (MQ=255)                                                                                                                                                                                                                                                  gATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGa  >  2:651234/1‑239 (MQ=255)                                                                                                                                                                                                                                                  gATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGa  >  1:1038544/1‑239 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     AAGCTTACCGGAATTAATCAGGCGCACCACCACAGCCTGTGAATCGTTGGGGAAAGAGTATGAGATCCTGCTGATTGATGACGGCAG‑TAGCGATAATTCC‑GCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAAC‑AGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTAT‑GGCGGGATTCAGT‑CACGTTACTGGCGACTTAATTATTACCCTT‑GATGCCGAT‑CTCCAGAATCCGCCAG‑AAGAAATC‑CCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAG‑AACCGCCAGGACAGCTGGTTTCGTAAAACCGC‑TTCGAAGATGATTAACCGGCTTATTCA‑GCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGA  >  NC_000913/2367131‑2367604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑