Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,367,380 G→A 12.1% Q104Q (CAG→CAA arnC → undecaprenyl phosphate‑L‑Ara4FN transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,367,3800GA12.1% 164.7 / 3.0 75Q104Q (CAG→CAAarnCundecaprenyl phosphate‑L‑Ara4FN transferase
Reads supporting (aligned to +/- strand):  ref base G (25/39);  new base A (3/6);  total (30/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGCGCACCACCACAGCCTGTGAATCGTTGGGGAAAGAGTATGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTT  >  NC_000913/2367149‑2367616
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 
cAGGCGCACCACCACAGCCTGTGAATCGTTGGGGAAAGAGGAGGAGACCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGc                                                                                                                                                                                                                                            <  1:533077/239‑1 (MQ=255)
    cgcACCGCCACAGCCTGTGAATCGATGGGGAAAGAGTAGGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGcc                                                                                                                                                                                                                                           <  1:672769/236‑1 (MQ=255)
           gtataggaaagagttCGTGGGGAAAGGAGTATGAGTTCCGGCTGATGGAGGACGGCAGGGGCGATAATTCCGCGAATATGCAGGTCGAAGCCCACCAGGGGGAAAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCAATTATGGCGGGATTCAGTCACGTTCCTGGCGACTTAATTATTCCCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATc                                                                                                                                                                                                                                <  1:530158/225‑1 (MQ=255)
                    gtgAATCGTTGGGGAAAGAGTATGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCtg                                                                                                                                                                                                                      <  2:1010417/239‑1 (MQ=255)
                      gAATCGTTGGGGAAAGAGTATGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTTAGTCACCGTTTTTGCGGCTTAATTATTTTCCTTTATGCCCCTCTCCAGAATTCCCCCGAAAAAATCCCCCCCCcggt                                                                                                                                                                                                                    >  2:444266/1‑239 (MQ=255)
                          cGTTGGGGAAAGAGTATGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCa                                                                                                                                                                                                                <  2:75532/239‑1 (MQ=255)
                                   aaGAGTATGAGTCCCTGCTGTTTGTTGCCGCAAGTAGCGATAATCCCGCCCATAGGCTGGTCGAAGCCTCACAGGGGGAGACCAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGCCACGTTACTGGCGACTTAATTATTCCCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGAt                                                                                                                                                                                                       <  1:47417/239‑1 (MQ=255)
                                   aaGAGTATGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTTAGTCACGTTACTGGCGACTTAATTTTTTCCCCTGATGCCGTTCTCCAAAATCCCCCAGAAAAAATTCCCCCCCcgggctgtttcttcta                                                                                                                                                                                                        >  2:530158/1‑225 (MQ=255)
                                      ggTATGAGAGCCGGCTTATTGACGCCGGCAGTTAGCAAAATTCCGGCGCATATGTTGTCCAAACCCCCAAAGGGGAAAAACAGCCATTTTGTTTCTATTTTGTTAAACCGAAATTCCGGGCAACATTCAGGGATTTGGGGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATg                                                                                                                                                                                                      <  1:11831/236‑1 (MQ=255)
                                      agTATGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTTATTCACGTTACTGGGGACCTTATTATTATCCTTTGTGCCGCTCCTCCCAATCCGCCCAAAGAAAACCCCCCCCCGGTGGCTAAAAcccctgga                                                                                                                                                                                                    >  1:1139454/1‑233 (MQ=255)
                                             tacccgcttgattaatGCCCGCAGGTGAGATATTCCCGCGAATATCCTGTGCTAAGCCTCTAAAGCGGAGACCAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGAAACATTCAGCGATTATGGGGGGATTCAGTAACGTTACTGGCGACTTAATTATTACCCTGGATGCCGAGCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacg                                                                                                                                                                                             <  1:43185/231‑1 (MQ=255)
                                               tcttgctgATTGAGACCGGCAGTGGCGATAATCCCGCGCATATGCTGGTCAAAGCCCCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacgac                                                                                                                                                                                           <  1:439051/236‑1 (MQ=255)
                                               tCCTGCTGATTGAGGACGGCAGTAGCAAAAATCCCGCGAATATCCTGGTGGAAGCCCCACAAGCGGAGACCAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacgac                                                                                                                                                                                           <  1:306655/239‑1 (MQ=255)
                                               tCCTGCGTATGGAGGACGGCGGTGGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACGAGCGGAGAACAGCCATATTGTGTTTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTAACGTTACTGGCGACTTAATTATTGCCCTTGATGCCGATCTCCAGAAACCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacgac                                                                                                                                                                                           <  1:589613/239‑1 (MQ=255)
                                               gccTGCTGATTGATGACGGCAGTAGCAATAATTCCGCGCATATGCTGGTCAAAGCCCCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacgac                                                                                                                                                                                           <  1:778735/238‑1 (MQ=255)
                                                ccTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGTTTCGTTCCCGTTCTGGGGGCCTTATTTTTTACCCTTGTGCCGGTCCTCCGAAATCCCCCGCAGAAAATCCCCCCCCCGGGGGCaaaaccgcagaagggttagac                                                                                                                                                                                            >  2:11831/1‑220 (MQ=255)
                                                ccTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGCTTTAGTCACGTTATTGGCGGCTTAATTATTAACACTTATGCCGCTCTCCAGAAACAGCCCAAAGAAAAACCCCCCCCGTGGGGAAAAACCCACGAAAGTTacgccg                                                                                                                                                                                          >  2:1140137/1‑239 (MQ=255)
                                                  tgctgATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGTGTTTAATCACGTTACTTGCGACCTAATTTTTTCCCTTTTTGCCGGTCTCCCGAAACCACCCGAAAAAAAACCCCCCCCTGGGGGCAAAACCGATGAAAGGTacgacggg                                                                                                                                                                                        >  1:918768/1‑237 (MQ=255)
                                                  tgctgATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTTATTCACGTTACTGGCGGCTTTATTATTATCCTTTTTTCCGATCTTCCGAATCCCCCCAAAGAAATCCCCCCCCTGGGGGCAAAAAACGATGaaaggtaacaagtg                                                                                                                                                                                        >  2:538376/1‑227 (MQ=255)
                                                  tgctgATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGGTACTGGCGACTTCATTATTACCCCTGATTGCCACCTCCAGGATCCCCCACAAGAAATCCCCCCCCTGGTGGCGAAAGCCGACGAAGGGTacaacggc                                                                                                                                                                                        >  2:255111/1‑237 (MQ=255)
                                                     tgattgatGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGt                                                                                                                                                                                      <  2:82400/238‑1 (MQ=255)
                                                       attgatGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTTAGTCACGTTACTGGCGACTTAATTTTTAACCTTTTTGCCGATCTCCCGAATCCGCCAGAAGAAAACCCCCGCCTGGTGGCAAAAGCCGATGAAGGGTacccactgggagg                                                                                                                                                                                   >  2:59281/1‑228 (MQ=255)
                                                        ttgatGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTCCCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAgg                                                                                                                                                                                   <  2:94127/238‑1 (MQ=255)
                                                         tgatgaCGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTTAGTCACGTTTCTGGCGGCTTTATTTTTAACCTTTATGGCGATCTTCAGAAAACGCCCGAAGAAATCCACCCCCTGGTGGCGAAAAACGATGAAGGTTACGACGGGGTAgggg                                                                                                                                                                                 >  2:399787/1‑238 (MQ=255)
                                                         tgatgaCGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGa                                                                                                                                                                                 <  2:512218/239‑1 (MQ=255)
                                                         tgaggaCGGCAGTGGCGATAATTCCGCGCATATGCTGGTCGAGGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGa                                                                                                                                                                                 <  1:618158/239‑1 (MQ=255)
                                                                ggCAGTAGCGATAATCCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACg                                                                                                                                                                          <  2:641864/239‑1 (MQ=255)
                                                                                                             aaGGGGAGAACAAGCATTTTTGTGTCTATTTGGCTTAACCCAAATTACGTGCAAGATTCAGCGATTATGGCGGGTTTCAGCCACGTTGCTGGCAACTTAATTTTTCCCCTTGATGCCGTACTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGaa                                                                                                                              <  1:930752/239‑1 (MQ=255)
                                                                                                                     accAGCAATTTTGTTTCTATTTTGCTTAACCGCAATTCCGGGAAACATTCAGCGATTAGGGGGGGATTCAGCCACGTTACTGGCGATTTAATTATTCCCCTGGATGCCAATCTCCAGAATCCGCCAGAAGAAACCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTaa                                                                                                                     <  1:59281/237‑1 (MQ=255)
                                                                                                                      accatctattttGTGTCTATTTTGCTTAACCGAAATTCCGGGCAACATTCAGCGATTATGGGGGGATTCAGTCACGTTACTGGCGACTTAATTATTCCCCTTGATGCCGATCCCCAGAATCCGCCAGAAGAAACCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTaa                                                                                                                     <  2:918768/229‑1 (MQ=255)
                                                                                                                      accagccttttgtggtCTATTTTGTTAAACCGAAATTACGGGAAACATTAAGCGTTTATGGGGGATTTCGTGCCCGTACCGGGCAATTTAATTATTCCCTTTGTGGCGGATCTCCAGAACCCCCGAGAAGAAACCCCCGGCCGGTGGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTaa                                                                                                                     <  1:903107/225‑1 (MQ=255)
                                                                                                                           cctttttGGGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGCCCCGTTACTGGCAACTTAATAATTCCCCTTGATGCCAATCCCAAGAACCCGCCAAAAGAAACCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGc                                                                                                                <  1:508671/233‑1 (MQ=255)
                                                                                                                              ttttGTGTGTTTTTTGCTTAACCCAAATACCGGGCACCATTCAGCGATTAGGGCGGAATTCAGTCACGTTCCTGGCGACTTAATTATCCCCCTGGAGGCCGATCCCCAGAATCCCCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTGGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTa                                                                                                             <  1:399787/237‑1 (MQ=255)
                                                                                                                                ttGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTTAGTCACGGTATAAGGGAATCAATTATTTATCTTTAAGCCGAGCTCCACAAACCCCCAGAAGGAATCACCCCCCCCGGGGGTAAAAACGAAGAAGGGTAAGCAGGGGGATGGACTGCACGCCAGAAACGCCAGGAAAGCGGGTTTCGTAACAACGCATTTGAAgaggataatcggcgttc                                                                                                             >  1:185516/1‑227 (MQ=255)
                                                                                                                                     tCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTCAGTCACGTTACTTGCGGCTTTATTATTACCCTTTATGCCGATCTCCAGAATCCCCCAGAAGAAATCCCCCCCCTGGTGGCAAAAACCCATGAAAGTTACGACGTGGGAGGGACTGTACGCCCGAACCGCCCGGACAGCCGGTTTCGTAAAACCCCTTCGAAGATGATTAACCGGCTTATTCatc                                                                                                       >  2:589919/1‑236 (MQ=255)
                                                                                                                                     tCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGGTTAGTCACGGTACCGGGGGCTTTATTTTTACTCTTTTTGCCGCTCCCCCGAATCCCCCACCAAAAAACCCCCCCCCGGTGGGAAAAACCGATCAAGGTTATGACGAGGGAGGGGGTGGACGCCAGAACACCCACGCCAACTCGTTTTGTTAAAACCCCTCGTAgaaggttcacacgcctcttctac                                                                                                       >  1:1149930/1‑217 (MQ=255)
                                                                                                                                     tCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTTAATCACCATTCTGGCGACTTAATTATTTCCCCTTATGCCGATGTCCAAAAACCCCCACAAAAAAACCCCCCCCTCGTGGCTAAAACCCAAGAAAGGTACCCCGTGGGAGGGGCAGTACGCCCGAAACACCAGGACAGGTGGGTTGTGAAAAAAGCATCGGAGAGGAATAAcacggttaattaga                                                                                                       >  1:36779/1‑225 (MQ=255)
                                                                                                                                     tCTATTTTCCTAACCCCCAATCGCGGCAACAATTAGCGGTTTTGGGAGGAATTCAGTCCCGTTTGGGTCGCCTTACTTCTTACCCTGTATCCCGTCCCCAAAAACCCCCCAGAAGAAACCCCCCCCCTGTTGGCAAAAGCAGATGAAGTTAACGACGTGTTAGGACCTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAgcg                                                                                                     <  1:298486/239‑1 (MQ=255)
                                                                                                                                     gtgatttgacTTACCCGCAATAACGGGCAAGATACAGCATTTAGGGCGGGATTCAGTCACGTTACGGACGACTAATTGTTTCCCCTTGAGGCCAAGCCCCAGATTCCGCAAGAAGAAACCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTGCGACGTGGAAGGGACTGTACGCCAGATCCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAgcg                                                                                                     <  1:255111/230‑1 (MQ=255)
                                                                                                                                               ttAAACGAAAATACGGGCAACATACATCGATTATGCGGGGATTCGTTCCCTTTGCTGGTGCTTTAATTTTTCCCGTGGAGTCCGATCTCCAGAACCCGCGAAAAAAAAGCCCCCGCCTGGTGGCAAAAGCCGATGAAGTTTACGATGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGc                                                                                           <  1:444266/239‑1 (MQ=255)
                                                                                                                                                 aaCCGCAATTACGGGCAACATTCAGCGATTATGGCGGTGTTTAGTCCCGTTACTTGCGACCTAATTATTATCCTTTTTGCCGATCTCCCGAATCCGCCAGCAGAAAACCCCCCCCTGGTGGCCCAAGCCGGCGAAAGTTACGACGTGGGTGGGACGGTTCGGCAGAAACCCCCAGGCCGCCGGGTTCCTTAAACCCCTCCCGAGAAGAGTAAACCGCTTATTCATGCGcacaacggcac                                                                                          >  1:333737/1‑234 (MQ=255)
                                                                                                                                                     gCAATTCCGGGCACATTTCAGGGATTATGGCGGGATCCAGCAACGTTCCTGGCAACTAAATTATTCCCCTTGAGGCCGATCCCCAAAACCCGCCAGAAAAATCCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTAGGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCg                                                                                     <  1:522021/239‑1 (MQ=255)
                                                                                                                                                         ttACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATgg                                                                                 <  2:583008/239‑1 (MQ=255)
                                                                                                                                                               caacaagtCAGCGTTTAGGGGGGGATCCAGTCAGTTTACTGGCGACTTAATTATTCCCCTGGAGGCCGATCCCCAGAACCCCCCAGAAAAAACCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGAt                                                                            <  1:538376/231‑1 (MQ=255)
                                                                                                                                                                aaaCAGTCAGGGATTAGGGGGGAAGCCAGCCGCGTTGTGGGCGACATAATTATTCCCCTGGTTGCCGAGCTCAGGAACCCGCCAGAAGAAATCCCCCGCCTGGTGGAAAAAGCCGAGGAAGGTTACGACGTGGTAGGGCCGGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTa                                                                          <  1:987187/238‑1 (MQ=255)
                                                                                                                                                                  aaaTTCAGCGTTTATGGGGGGTTTCAGCAACGTTCCTGGGGATTAATTTTTTCCCCTGGAGGCCGATCCCAAGAATCCCCAGGAAGAATCCCCCCGCCGGGGGGCAAAACCCGATGAGGGTTACGAGGTGGTAGGGACTGTCCGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACg                                                                        <  1:589919/237‑1 (MQ=255)
                                                                                                                                                                           ggttAGGGCGGAATTCAGCCGTGTTCGTGCGGCTTAAATTATACCCTTAGAGGCCGATCTCCAAAACCCTCCAAAAGAAATCCCCCGCCGGGTGGAAAAAGCCGATGAAGGTTACGACGTGGTGGGGCCTGTACGCCAGACCCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTa                                                                  <  1:1206403/234‑1 (MQ=255)
                                                                                                                                                                              tATGGCGGGATTCAGTCACGTTCCTGGCGACTTAATTTTTCCCCTTGATCCCGATCTCCAGAAACCGCCAGAAGAAACCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTGGGGATTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTAtgctgc                                                            <  2:928314/239‑1 (MQ=255)
                                                                                                                                                                                        aTTCAGTCACGTGACGGGAGATTTAATTATGCCCCTGTGTGCAGATGTCCAGAACCCGCCAGAAGAAATCCCCCGCCTGGGGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTAt                                                    <  1:1070730/238‑1 (MQ=255)
                                                                                                                                                                                                  cGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAACCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCata                                          <  2:1139454/239‑1 (MQ=255)
                                                                                                                                                                                                   gTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTa                                                                          >  1:720222/1‑206 (MQ=255)
                                                                                                                                                                                                   gTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTa                                                                          <  2:720222/206‑1 (MQ=255)
                                                                                                                                                                                                       cTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTc                                     >  2:297502/1‑239 (MQ=255)
                                                                                                                                                                                                        tGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTcg                                    >  1:460712/1‑239 (MQ=255)
                                                                                                                                                                                                         ggCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAGGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGGAGGGACGGTACGCCAGAACCGCCAGGACAGGTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCTCACCACTGGCAAAGCGATGGGTGATTACGGTGGTATTCTTCGCGCCTCTCGCCGTCATATTGTcgt                                   >  1:236727/1‑238 (MQ=255)
                                                                                                                                                                                                            gACTTAATTATTCCCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTcgatg                                 <  2:845536/238‑1 (MQ=255)
                                                                                                                                                                                                             aCTTATTTATTCCCCTGGATGCCGATCCCCAAAAGCGGCAGAAAGAAACCCCCCGCCTGGTGGCAAAAGCCGATGAAGTTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTcgatgcg                               <  1:297560/239‑1 (MQ=255)
                                                                                                                                                                                                             aCTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCCTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTcgatgcg                               >  2:872031/1‑239 (MQ=255)
                                                                                                                                                                                                               ttAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTcgatgcgat                             >  2:475296/1‑239 (MQ=255)
                                                                                                                                                                                                                     attcCCCTTGATGCCGATCTCCAAAATCCCCGAGAAGAAATCCCCCGCCTGGTGCCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACGGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCa                       <  2:185516/239‑1 (MQ=255)
                                                                                                                                                                                                                       tccccGTGATGCCAATCGCCGAAACCCGCCAAAAAAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACt                     <  2:518576/237‑1 (MQ=255)
                                                                                                                                                                                                                        aCCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACt                     >  2:158310/1‑238 (MQ=255)
                                                                                                                                                                                                                           cTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCa                 >  2:896004/1‑239 (MQ=255)
                                                                                                                                                                                                                               gatccggATCTCCGGAATCGGCCAAAAGAAATCCCCCGCCGGGTGGCAAAGGCCGATGAAGGTTAGGAGGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATg               <  2:553118/232‑1 (MQ=255)
                                                                                                                                                                                                                               gATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGa              >  2:651234/1‑239 (MQ=255)
                                                                                                                                                                                                                               gATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGa              >  2:651384/1‑239 (MQ=255)
                                                                                                                                                                                                                               gATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGa              >  1:1038544/1‑239 (MQ=255)
                                                                                                                                                                                                                                    cGATCTCGAGAATCCGCCAGAGGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGAGGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACgc          <  2:642276/238‑1 (MQ=255)
                                                                                                                                                                                                                                     gATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTaa                                                                                                                     <  2:589341/131‑1 (MQ=255)
                                                                                                                                                                                                                                     gATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTaa                                                                                                                     >  1:589341/1‑131 (MQ=255)
                                                                                                                                                                                                                                     gATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGCTGCGATGTTGCACTGCCATGAACgca         >  2:840508/1‑238 (MQ=255)
                                                                                                                                                                                                                                     gATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACgca         >  2:752005/1‑238 (MQ=255)
                                                                                                                                                                                                                                      aTCTCAAGAACCGTCCAGAAGAAATCCCCCGCTTGGTGGCAAAAGCCGATGAAGGTTCCGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACgcag        <  2:481302/238‑1 (MQ=255)
                                                                                                                                                                                                                                       agcgccAGAACCCGCCGGAAAAAACCCCCCGCCGGGTGGCAAAGGCCGAGGAAGGTTACGATGTGGTAGGGACTGTAGGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACgcag        <  1:475296/234‑1 (MQ=255)
                                                                                                                                                                                                                                            cAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCtt  >  1:514367/1‑239 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 
CAGGCGCACCACCACAGCCTGTGAATCGTTGGGGAAAGAGTATGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTT  >  NC_000913/2367149‑2367616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: