BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,206,481	G→A	22.6%	K7K (AAG→AAA)	ttdA →	L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,481	0	G	A	22.6%	180.3 / 26.6	119	K7K (AAG→AAA)	ttdA	L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand): ref base G (17/73); new base A (23/3); total (40/79)
Fisher's exact test for biased strand distribution p-value = 1.13e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

ATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCC‑AGACAAAT‑ACAAAAACT‑GGAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCC  >  NC_000913/3206246‑3206716

aTTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCC‑AGACAAAT‑ACAAAAACT‑GGAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCaa                                                                                                                                                                                                                                            <  2:975910/239‑1 (MQ=255)

aTTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCC‑AGACAAAT‑ACAAAAACT‑GGAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCaa                                                                                                                                                                                                                                            <  2:62281/239‑1 (MQ=255)

 ttCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCC‑AGACAAAT‑ACAAAAACT‑GGAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCAAc                                                                                                                                                                                                                                           <  2:1068312/239‑1 (MQ=255)

 ttCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCC‑AGACAAAT‑ACAAAAACT‑GGAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCAAc                                                                                                                                                                                                                                           <  2:367731/239‑1 (MQ=255)

       aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCC‑AGACAAAT‑ACAAAAACT‑GGAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCAACAgg                                                                                                                                                                                                                                        <  2:275370/236‑1 (MQ=255)

       aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCC‑AGACAAAT‑ACAAAAACT‑GGAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCAACAGGc                                                                                                                                                                                                                                       <  2:218379/237‑1 (MQ=255)

       aTCGTTAATTATCCGCACGTGTGATAAGCGAAGTGTATTTCGCAAAACATAGCCATTCCCTGTTCTGGCGCAATATTCATTCTTATTTTCGCGTTGAGTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACAGTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCC‑AGACAAAT‑ACAAAAACT‑GGAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCAACAGGc                                                                                                                                                                                                                                       <  1:990895/237‑1 (MQ=255)

         cGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTC‑AGACAAAC‑ACAAAAAAG‑TGAGATGGCATGATT‑A‑GGGAAAGGAAAAAAAAAAAGGcggca                                                                                                                                                                                                                                   >  2:530927/1‑235 (MQ=255)

              aTTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCC‑AGACAAAT‑ACAAAAACT‑GGAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCAACAGGCAGTGAATaa                                                                                                                                                                                                                              <  2:466571/239‑1 (MQ=255)

              aTTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCC‑AGACAAAT‑ACAAAAACT‑GGAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCAACAGGCAGTGAATaa                                                                                                                                                                                                                              <  2:1012099/239‑1 (MQ=255)

                    cGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTC‑AGAAAAAT‑AAAAAAACA‑TGAGGTGCCATTATGAA‑GCG‑AAGAAATAAACCAAAGGCAATGAAAAAATTGaa                                                                                                                                                                                                                        >  2:877394/1‑238 (MQ=255)

                         ttGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGA‑TTTTTTC‑CAACAAATAAAAAAAACT‑GG‑GTTGTCCTTGTG‑G‑GGGGAAGTAATAAGCAACAAGCCGGGAAAAAATTGGCAggga                                                                                                                                                                                                                   >  2:513028/1‑239 (MQ=255)

                                 aGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGA‑TTTTTTC‑TTACCAAA‑AAAAAAAAT‑GGAGGTGCCCTTGTG‑G‑GCGAAAAAAATAAACAACAAGGAGAGAAAAAGTTGACAGAGAGTGTTGCt                                                                                                                                                                                                           >  2:803893/1‑239 (MQ=255)

                                 aGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCC‑AGACAAAT‑ACAAAAACT‑GGAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                                                                                                                                                                           <  2:763793/239‑1 (MQ=255)

                                 aGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCC‑AGACAAAT‑ACAAAAACT‑GGAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                                                                                                                                                                           <  1:1055200/239‑1 (MQ=255)

                                   cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTC‑AAAACACA‑ACAAAAAAT‑GGAGTTGGCATGATG‑A‑GCGAAAATAATAAAGAACAAACAAAGAAAAAATTGAAAAAGAATGTCGGTaa                                                                                                                                                                                                         >  1:763793/1‑239 (MQ=255)

                                        tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGA‑TTTTTTC‑AGACAAAT‑ACAAAAACT‑GGAGTTGGCATGATG‑A‑GCGAAAGAAATAAACAACAGGCAGAGAAAAAATTGACAGAGAATGTCGCTAAAtttt                                                                                                                                                                                                    >  2:944415/1‑238 (MQ=255)

                                        tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAA‑ATTTTTA‑AAACAAAA‑AAAAAAAAT‑GGAGTTTGCATTGAG‑G‑GAGGAATGAATAAGCAAAAAAAAGGGAAAAAGATGACAGCAAATGTCACTAACtttt                                                                                                                                                                                                    >  1:660806/1‑238 (MQ=255)

                                             ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCC‑GAAAAAAA‑AAAAAAATT‑GGAGTTG‑CATGATG‑AGGCGAAA‑TAATAAACAAAAAGAAAGGGATAAAGTGAAAGAAAATGTCGATAACTTT‑aacac                                                                                                                                                                                               >  1:475308/1‑234 (MQ=255)

                                             ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTC‑AGACAAAA‑AAAAAAACA‑GGAGTTTCCATGTTG‑G‑GCGAAAGTAATAAACAACACGCAGGGAATAAAATGACAGAGATTGTCGCTAACTTT‑Acatcc                                                                                                                                                                                              >  2:1158443/1‑235 (MQ=255)

                                               cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTC‑CGACAAAT‑ACAAAAAAT‑GGGGTTGGCATTGTT‑G‑GGGAAAGGAATAAAAAACAAGCAGGGGATAAGTTTTCAGAGAATTTCGCCAAATTT‑TCccccca                                                                                                                                                                                            >  2:834286/1‑237 (MQ=255)

                                                  aaaaCATTGCGCTCCCCTTCTGGTGCAAAAAATTTATTCTTATTTTCGCGTGGATTCACAAACTTTAAATGAAATATTCACGGGGGAAAAAAATATAAAACCACATTTTTGGGGGTGTGTGGGGGCGGGAAA‑TTTTTCC‑AAAAAAAT‑AAAAAAACG‑GGAGTTGCCATGATG‑A‑GCAAAAGTAAAAAGCAACAGGAGGTGAATAAGTTGACAGAGATTGTCGCTAAATTC‑ACCGCCATGAt                                                                                                                                                                                         <  1:785710/239‑1 (MQ=255)

                                                  aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTC‑AGACAAAT‑ACAAAAACT‑GGGGTTGCCATGATG‑A‑GGGAAAGTAATAAGAAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGAt                                                                                                                                                                                         >  2:618666/1‑239 (MQ=255)

                                                    aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTC‑AGAAAAAA‑AAAAAAAAA‑GGGGGTGCCATGATG‑A‑GGGAAAGAAATAAAAAAAAAGGAGTGAATAAGTTGACAGAGATTTTTGCTAAATTT‑ACCGCCATGAttt                                                                                                                                                                                       >  2:190459/1‑239 (MQ=255)

                                                    aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTC‑AAACAAAA‑AAAAAAAAT‑GGAGTTTCCCTTATT‑A‑GGGGAAGTAATAAACAAAAAGCAGGGAATAAATTGTAAAAGAATTGCGCTAAAttttaccccctagttt                                                                                                                                                                                        >  2:647802/1‑226 (MQ=255)

                                                      cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTC‑AGACAAAA‑AAAAAAAAT‑TGAGTTGCCATGATG‑A‑GGGAAAGTAATAAACAACAGGGAGTGAATAAATTGACAGAGATTGTTGCTAACTTT‑ACCGCCCTTAttttt                                                                                                                                                                                     >  2:847286/1‑237 (MQ=255)

                                                            cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCC‑AGACAAAT‑ACAAAAACT‑GGATTTGCCATGATG‑A‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGa                                                                                                                                                                               <  2:441689/239‑1 (MQ=255)

                                                            cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTACGCTTTGATTCACAAACTGTTAATTACATATTCACGGGGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAA‑TTTTTCC‑AGAAAAAT‑ACAAAAACT‑GGAGTTGCCATGATG‑A‑GCGAAATAAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGa                                                                                                                                                                               <  1:422381/239‑1 (MQ=255)

                                                             aTTCCCTGCTCTGGCGCAATATTAATTCTTATTTTCGCGTTGATTCACAAACTGTTAATTACATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCC‑AGACAAAT‑ACAAAAACT‑GGAGTGGCCATGATG‑A‑GCGAAAGTAATAAGCAACAGTCAGTGAATAAGTTGACAGAGATTGTCGTTAACTTT‑ACCGCCATGATTTCTACCCGaa                                                                                                                                                                              <  1:707159/239‑1 (MQ=255)

                                                              ttCCCTGCTCTGGCGCAATATTCTTTTTTATTTTCGTGTAGATTCACGAACTTTTAATTAAATATCCGCGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTATTTTGTGGCGGTAAT‑TTTTTCA‑AGACAAAT‑ACAAAAACT‑GGAGTGGCCAGGATG‑A‑GCAAAAGTAATAAGAAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAAt                                                                                                                                                                             <  1:834286/239‑1 (MQ=255)

                                                               tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAA‑ATTTTTC‑AAAAACAA‑ACAAAAAAT‑GGAGTTTCGAAGAAG‑A‑GCGAAAATAATAAACAACAAGCAGTGAATAAATTTACAGAGATTTTCACTAAATTT‑ACAACCATGATTTCtcacacaat                                                                                                                                                                             >  1:1136401/1‑230 (MQ=255)

                                                                cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTC‑AGAAAAAT‑ACAAAAACT‑GGAGGTGTCATGATG‑G‑GAGAAAGGAATAAACAAAAAGCAGTGAATAAAGTGAAAGAGATTGTTGCTAAATTT‑TACGCCATGATTTTTTCCCCAATg                                                                                                                                                                            >  2:422381/1‑238 (MQ=255)

                                                                     ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGA‑TTTTTTC‑AGACAAAA‑ACAAAAACT‑TGAGTTGCCATGATG‑A‑GGGAAAGGAATAAGAAACAGACAGTGAATAAGTTGACACAGATTGTCGCTAAATTT‑TCCCCCCAGATTTCTACCCCAATGCCtgat                                                                                                                                                                      >  2:1089593/1‑239 (MQ=255)

                                                                          gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTC‑AGACAAAA‑ACAAAAAAT‑GGAGTTGCCATGATG‑A‑GCGGAAGTAATAAACAAAAAGCAGTGAATAAATTTACAAAGATTGTTGCTAACATT‑ACCGCCATGATTTTTACCCGAATGCCTGGTGACgt                                                                                                                                                                 >  2:374205/1‑239 (MQ=255)

                                                                               atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTC‑AGACAAAT‑ACAAAAAAT‑GGAGTTGCCATGATG‑A‑GCGGAAGTAATAAACAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ATCGCCATGATTTCTACCCGAATGCCTGATGACGtggggg                                                                                                                                                            >  2:553213/1‑239 (MQ=255)

                                                                                ttttaatttTTATTATCGGTTTGATTCACAAACTGTTAATTACATATTCGTGGGGGAAAAAAAAAAAAAACCACATTTTTGGGTGTAGTTTGTGGCGGTGAA‑TTTTCCC‑AGAAAAAT‑AAAAAAACG‑GGAGTTGCCATGATG‑A‑GCAAAAGTAAAAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                           <  1:1037681/234‑1 (MQ=255)

                                                                                tatttattTTTATTGTAGGTTTGATTCACGAATTGTTAATTAAATTTTCAGGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGGGGGGGGAAA‑TTTTTCA‑AAAAAAAT‑AAAAAAACG‑GGGTTTGCCATGATG‑A‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGTTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                           <  1:647802/239‑1 (MQ=255)

                                                                                tattatttcTTATTTTGACTTTAATTCACAAACTGTTAATTAAATATTCGGGGGGCAAAAAAAAAAAAAACCACATTTTTAGTGGTATTTTGTGGCGGTAAT‑TTTTTCA‑AAAAAAAT‑AAAAAAACT‑GGAGTTGCCTTGAGG‑A‑GCGAAATTAAAAAGCAACAGGCAGTGAATAAGTTGACAGATATTGTCGCTAATTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                           <  1:526835/233‑1 (MQ=255)

                                                                                        cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATGCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCC‑AAACAAAT‑AAAAAAACT‑GGAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa                                                                                                                                                   <  2:877032/239‑1 (MQ=255)

                                                                                          tATTTTCGGGTTGATTCACGAACTGTTAATTACATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTAAA‑TTTTTCC‑AGACAAAT‑ACAAAAACG‑GGAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                 <  2:1166046/239‑1 (MQ=255)

                                                                                          tATTTTCGCGTTGATTCACGAACTTTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTCCC‑AGACAAAT‑ACAAAAACT‑GGAGTTGCCATGATG‑A‑GCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                 <  2:724782/239‑1 (MQ=255)

                                                                                          tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑ATTTTTA‑AAACAAAT‑ACAAAAAAT‑GGAGGTTGCATGGTG‑G‑GAGAAAGTAAATAGCAACAAGAAGAGAATAAGATGACAGAGATTGTAGCTCAATTT‑AACGCAACGGATTTTAAACGAATGCCTGATATAGCGGTGAGGAAACTaaaa                                                                                                                                                 >  1:686417/1‑239 (MQ=255)

                                                                                          tATTTTCGCGTTGATTCACAAACTGTTAATTACATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTATTTGGTGGCGGTGAA‑TTTTTCC‑AGACAAAT‑AAAAAAACG‑GGAGTTGCAAGGATG‑A‑GCGAAAGTAATAAGCAACAGGCAGAGAATAAGTTGAAAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                 <  2:980562/239‑1 (MQ=255)

                                                                                                 gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTC‑ATACAAAA‑AAAAAAACT‑TGAGTTGCCCTGATG‑A‑GCGAAAGTAATAAACAACAAGCAGTGAATAAATTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTTTAACCCAATGCCTGATTACGTGGGGGGTAAACTACAACAACTaa                                                                                                                                          >  2:459662/1‑239 (MQ=255)

                                                                                                    ttGATTCACGAACTGTTAATTACATTTCCACGGTGGCAAAAAATAAAAACCCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCC‑AGACAAAT‑ACAAAAACT‑GAATTTGCCATGATG‑A‑GCAAAATTAATAAGCAACAGGCAGTAAATAAGTTAACAGAATTTGTCGTTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAg‑g                                                                                                                                      <  2:770039/239‑1 (MQ=255)

                                                                                                     gaattaccgactttttaattaaATTTTCCTGGTGGAAAAAAAAAAAAAACACCTTTTTAAGGGGTAGTTGTGGGCGGGAAG‑TTTTTCA‑AAACAAAA‑ACAAAAAAG‑GGTGTGGCAATTATG‑A‑GTGAATGAAATAAACAACGGGCATTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAg‑g                                                                                                                                      <  1:851108/224‑1 (MQ=255)

                                                                                                                   ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTATTTTGTGGCGGTAAA‑TTTTTCC‑AGACAAAT‑AAAAAAACT‑GGAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAAcgtcgt                                                                                                                       <  2:1046637/239‑1 (MQ=255)

                                                                                                                       ttACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCC‑AGACAAAT‑ACAAAAACT‑GGAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGAt                                                                                                                   <  2:972372/239‑1 (MQ=255)

                                                                                                                       ttAAATATTCACGGTGAAAAAAAATAAAAAACCACATTTTTAGGGGTAGTTTGGGGCGGTGAA‑TTTTTCC‑AGAAAAAA‑ACAAAAACT‑GGAGTTGCCAGGATG‑A‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGAt                                                                                                                   <  1:994363/239‑1 (MQ=255)

                                                                                                                           atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑ATTTTTT‑AAACAAAA‑ACAAAAAAT‑TGAGTTTCCCTGATT‑A‑GCGAAAGTAATAAACAACAAGCAATGAATAAAATGAAAGAGAATGTTGCCAACTTT‑ACCCCCCACATTTTTTACCCCATGCCTGATGACGTGGTGGGTAAACTAAAAAAAACAAAA‑GATGCCCGAACGCCGTCGGTgggg                                                                                                               >  1:9497/1‑239 (MQ=255)

                                                                                                                            ttttaagggTGGAAAAAAATAAAAAACCAAATTTTTAGTGGTAGTTTGTGGAGGTGAA‑TTTTTCA‑AGAAAAAA‑AAAAAAACG‑GGAGTTGCAATGATG‑A‑GCAAAAGTAATAAGCAACAGGCAGTGAATAGGTTGACAGAGATTGTGGTTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGa                                                                                                              <  1:618666/232‑1 (MQ=255)

                                                                                                                            tatTCCCGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAA‑TTTTTCC‑AGACAAAT‑ACAAAAACT‑GGAGTTGCCATGATG‑A‑GCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGa                                                                                                              <  2:593341/239‑1 (MQ=255)

                                                                                                                            tatTCACGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCC‑AGAAAAAT‑AAAAAAACT‑GGAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCAACAGGAAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGa                                                                                                              <  2:1193615/239‑1 (MQ=255)

                                                                                                                             atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGA‑TTTTTTC‑AAACAAAT‑ACAAAAAAT‑TGAGTTGCCATGATG‑A‑GCGGAAGTAATAAGCAACAAGCAGGGAATAAATTGACAGAGATTGTCGCTAACTTT‑TCCCCCACGATTTTTACCCGAATGCCTGATGAAGTGGGGGGTAAAAATAAACAACTAAAGAGAGGCCCAAAccaggcgggtgggga                                                                                                              >  2:834004/1‑225 (MQ=255)

                                                                                                                             atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTC‑CGACAAAA‑ACAAAAACT‑GGAGTTGTCAAGATG‑G‑GCGGAAGGAATAAAAAAAAAGCAGAGAATAAATTGACAGAGATTTTTGCTAACTTT‑ACCGCCATGATTTTTACCCGAATGCCTGATGACGGGGGGGGTAAACTAAAAAAGCTAAAA‑GATGCCCaaaaagcggcgcaggggga                                                                                                             >  2:414315/1‑223 (MQ=255)

                                                                                                                             atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTC‑AGACAAAT‑ACAAAAAATGGGAGTTG‑CATGGTG‑G‑GCGGAAGGAATAAGCAAAAAGCAGTGAAAAAATTGACAAAGATTGTCGCTAAATTT‑TACGCCCTGAATTCTAACCCAATGCCTTATGACGTGGGGGGTAAAATAAAACAACTAAAA‑GAAGCCCAAAAGTACTCGATggggga                                                                                                             >  2:789931/1‑237 (MQ=255)

                                                                                                                               tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGA‑TTTTTTC‑AGACAAAT‑ACAAAAAAA‑GGAGTTGGCATTATT‑G‑GCGAAAGAAAAAAGCAACAAGCAGTGAATAAATTTACAGAAAATGTCGCTAACTTT‑ACCCCCACGATTTTTACCCGAATGCCTGATGACGTGGTGGGTAAACTAAAACAACCAAAA‑GATGCCGAAACACCGTCGCTGGGGgaaa                                                                                                           >  2:1128967/1‑239 (MQ=255)

                                                                                                                               tCACGGGGAAAAAAAATAAAAAACCAAATTTTTAGTGGTAGTTTGGGGCGGTGAA‑TTTTTCC‑TGAAAAAA‑AAAAAAACG‑GGAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTCGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGaaaa                                                                                                           <  1:803893/239‑1 (MQ=255)

                                                                                                                                 aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑ATTTTTC‑AGACAAAA‑AAAAAAACT‑TGAGGTGTCCTGGTG‑G‑GCGAAAGTAAAAAGAAACAGACAGAGAATAAGTTGACAGAGAGTGTCGGTAAATTT‑ACCGCCCTGATTTCTACCCGAATACCTGATTAAGTGGTGGTTAAACTAAAACAACTAAAG‑AATGACGAAACGTCGTCGGGGGGGgaaaat                                                                                                         >  1:162693/1‑237 (MQ=255)

                                                                                                                                 aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑ATTTTTC‑AAAAAAAA‑AAAAAAAAA‑GGGGGTGGCATGAGG‑A‑GCGGAAGAAAAAAAAAAAAGAAAGAGAAAAAAATGAAAGAGATTGTTGCTAACTTT‑ACAGCCAGGATTTCTTCCAGAATGACGGGTGAGGGGGGGGGTAAAATAAAAAAACTAAAA‑GATGCCGAAAAACGGTCGGTGGGGgaaaat                                                                                                         >  1:947587/1‑237 (MQ=255)

                                                                                                                                    gtgaaaaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTAAA‑TTTTCCC‑AGACAAAT‑ACAAAAACG‑GGAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGGCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAAtt                                                                                                         <  2:697045/231‑1 (MQ=255)

                                                                                                                                    gTGGAAAAAAAAATAAAACCACATTTTTAGTGGTAGTTGGTGGCGGTGAA‑TTTTTCA‑AAAAAAAT‑ACAAAAACG‑GGAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCAACAGGTAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                       <  1:1210753/238‑1 (MQ=255)

                                                                                                                                    gTGGAAAAAAAAAAAAACCCACATTTTAGGTGGGAGTTGGTGGGGGAAAA‑TTTTCAC‑AGACAAAT‑AAAAAAAGT‑GGAGTTGCCATGATG‑A‑GCAAAATAAATAAGCAACAGGCAGTGAATAAGTTAACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                       <  1:355353/238‑1 (MQ=255)

                                                                                                                                       gaaaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTCCC‑AGACAAAT‑ACAAAAACT‑GGAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                           <  2:462259/182‑1 (MQ=255)

                                                                                                                                       gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTC‑AGACAAAT‑ACAAAAACT‑GGAGTTGCCCTGATG‑A‑GCGAAAGGAATAAGCAAAACGCAGGGAATAAAGTGGCAGAGATTGTGGCTAAATTT‑TCCCCCACGATTTCTACCCCAATGGCTGATGACGGGGTGGGTAAACTAAAAAAGCTAAAA‑GATGCCGAAAAGCCGTCGGTGGGGGAAATTATTTa                                                                                                    >  2:844952/1‑238 (MQ=255)

                                                                                                                                           aaaaTTTAAACCCACATTTTTTGT‑GTTGTTTGTTGGGGGGAA‑TTTTTCCAAAAAAAAT‑AAAAAAACT‑GGAGTTGCCATGATG‑G‑TCGAAAGATATAAGCAACAGCCGTTGAAAAAGTAGACAGGTATTGTAGTTAATTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATa                                                                                               <  1:965279/239‑1 (MQ=255)

                                                                                                                                             aaTATAAAACCAGAGTAATAGTGTTGTTTTTGGGCGATGAA‑TTTTTCC‑AGACAAAT‑ACAAAAACG‑GAAGTAGCAATGATG‑A‑CGAAAAGTAATAGGAAACGGGCAGTCAATAATTTGACGGAGTTTGTGTAAAATTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                             <  2:48150/239‑1 (MQ=255)

                                                                                                                                             aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAA‑TTTTTCC‑AGACAAAT‑ACAAAAACT‑GGAGTTGCCATGATG‑A‑GCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                             <  2:162693/239‑1 (MQ=255)

                                                                                                                                             aaTATAAAACCACAGTTTTGTTGGTAGTTTGTGG‑GGTGAA‑ATTTTCCAAGACAAAT‑AAAAAACCT‑GGGGTTGCTATGATG‑A‑GCGAAAGAAATACGCAACAGGGGGTAAATAAGTTGACAGGGATTGTCTATAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                             <  1:1158443/239‑1 (MQ=255)

                                                                                                                                             aaTAAAAACCCAAATTTTTAGTGGTAGTTTGGGGCGGTGAA‑TTTTTCA‑AAACAAAA‑ACAAAAACT‑GGAGTTGCAATGATG‑A‑GCGAAAGAAAAAAGAAACAGGCAGTAAATAAGTTAACAGAGTTTGTCGCTAATTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                             <  1:1223418/239‑1 (MQ=255)

                                                                                                                                                      ccACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTCCC‑AGACAAAT‑ACAAAAACG‑GGAGTTGCCATGAGG‑A‑GCAAAAATAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGATAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGac                                                                                    <  2:7546/239‑1 (MQ=255)

                                                                                                                                                                      atttgttgGGGGTGAA‑TTGTTCC‑AGACAATT‑ACAAAAACA‑GGAGTTGCCATAATG‑A‑GGTAAAGTAATAAGCAACAGGCAGGAAATAAGTTGACAGGTATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                    <  2:452729/233‑1 (MQ=255)

                                                                                                                                                                      aGTTTGTGGCGGTGAA‑TTTTTCC‑AGACAAAT‑ACAAAAACT‑GGAGTTGCCATGATG‑A‑GCGAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                    <  2:660806/239‑1 (MQ=255)

                                                                                                                                                                       gTTTGTGGGGGTGAA‑TTTCACG‑AAACAAAT‑ACAAAAACT‑GGAGTTGCCAAGATG‑A‑GCAAAAGAAATAAACAAGGGGCGTTGAAAAAGTGAACAGATATTGTCGATACTTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                    <  1:14921/238‑1 (MQ=255)

                                                                                                                                                                       gTTTGTGGCTGTAAA‑TTTTTCA‑AAAAAAAA‑AAAAAACCT‑GGAGTGGCCATGAGA‑T‑GCAAAAGAAAAAAGCAACAGGCAGTAAATAAGTTGACAGAGTTTGTCGTTAATTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                    <  1:1089593/238‑1 (MQ=255)

                                                                                                                                                                           gtgGCGGTGAA‑TTTTTCC‑AGACAAAT‑ACAAAAACT‑GGAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAc                                                               <  2:1037511/239‑1 (MQ=255)

                                                                                                                                                                            tgGCGTTAAA‑TTTTTCA‑AAAAAAAT‑ACAAAAACT‑GGAGTTGCCAAGATG‑A‑GAGAAAGAAATAAGCAACGGGCAGTGAATAAGTGGAAGGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAc                                                               <  1:459662/238‑1 (MQ=255)

                                                                                                                                                                            tgGCGGAAAA‑TTTTGAC‑AGAAAAAT‑ACAAAAACT‑GAAGGGGCAATGATG‑A‑GCGAAAAAAATAAGCAAAAGGCGTTGAATAAGTGGAAAGAAAGTGTCGAAACCTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGGCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAc                                                               <  1:712962/238‑1 (MQ=255)

                                                                                                                                                                             ggggggtaattttttCC‑AACAAAAA‑ACAAAAACG‑GGAGTGGCCATGATG‑G‑GCGAAAGAAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAc                                                               <  2:389799/228‑1 (MQ=255)

                                                                                                                                                                                       tttttCC‑AGAAAAAA‑AAAAAAACT‑GGAGTTGCCATGATG‑A‑GCGAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGAAAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcc                                                    <  2:376748/239‑1 (MQ=255)

                                                                                                                                                                                         ttccc‑AGACAAAT‑ACAAAAACT‑GGAGTGGCCATGATG‑A‑GCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAAATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                  <  2:1136401/236‑1 (MQ=255)

                                                                                                                                                                                           ttacaaaaaaaa‑acaaaaacG‑GGGTTTGCCATGGAA‑G‑GCAAAAGAAAAAAACAAAAGGCAGTGAATAAGTTGAAAGAGATTGGCGCTAATTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgc                                                 <  1:847286/231‑1 (MQ=255)

                                                                                                                                                                                           tCC‑AGAAAAAT‑AAAAAAACT‑GGATTTCCAATGATG‑A‑GCGAAAGTAAAAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGATAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcc                                                <  2:244049/239‑1 (MQ=255)

                                                                                                                                                                                               aGAAAAAA‑ACAAAAATT‑GGAGTTGCCAAGATG‑A‑GCGAAAGTAAAAAGCAACAGGAAGTGAAAAAGTTGACAGAGATTTGCGCTAATTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTTCCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGt                                             <  2:817651/239‑1 (MQ=255)

                                                                                                                                                                                                     aaaaaaaaaaCT‑GGAGTTGCCAGGAGG‑G‑GCAAAAGAAAAAAGAAAGAGGCAGAAAATAAGTTGAAAGAGATTGTCGTAAATTTA‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGa                                        <  1:414315/234‑1 (MQ=255)

                                                                                                                                                                                                        aCAAAAACG‑GGAGTGGCCATTATG‑A‑GCGAAAGTAAAAAGCACGAGTGAGTGAATAATTTGACAGATATTGTCGCTAATTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGacac                                     <  1:1000215/239‑1 (MQ=255)

                                                                                                                                                                                                          aaaaaGT‑GGAGTTTCCATGAGG‑A‑GAGAAAGTAAAAACCAAAAGTGAGTAAATAAGTTGACAAAGGTTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCg                                   <  1:1150314/239‑1 (MQ=255)

                                                                                                                                                                                                           aaaaCT‑GGAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgg                                  <  2:9497/239‑1 (MQ=255)

                                                                                                                                                                                                            aaaCT‑GGAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg                                 >  2:850849/1‑239 (MQ=255)

                                                                                                                                                                                                             aaCT‑GGGGTGGCAATGATG‑A‑GCAAAAGAAAAAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAATTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                <  1:513028/239‑1 (MQ=255)

                                                                                                                                                                                                             aaCT‑GGATTTGCCATAATG‑A‑GCAAAAGCAAAAAGCAACAGCATTTCAATAAGTTAAAAGAGATTGTCGTTAGTTTT‑ACCGCCATGATTTCTACCCGAATGCCTTATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGTAACGTCGTCGATGGGGAAAATTATCTCCCAGCCGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                <  2:732324/239‑1 (MQ=255)

                                                                                                                                                                                                              actgggAGTTGCCAGGAAG‑A‑GCGAAAGTAATAAGCAAAAGGGAGTAAATAAGTGGACAGAGATTGCCCTTATCTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                <  1:553213/235‑1 (MQ=255)

                                                                                                                                                                                                                  ggAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAt                            >  1:1115850/1‑239 (MQ=255)

                                                                                                                                                                                                                    aGTTGCCATGATG‑A‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTa                          >  1:992747/1‑239 (MQ=255)

                                                                                                                                                                                                                        gCCATGATG‑A‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTATCTTT‑ACCGCCATGACTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGTAAAATTATCTAACATACGATGTTCTACAACATGCAAAAAGCGATTGAACTGACTCGTCATGCCTGTCAGGACACCGGGGAGATTATGtt                      >  1:1054914/1‑239 (MQ=255)

                                                                                                                                                                                                                             gatg‑a‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCg                 >  1:354023/1‑239 (MQ=255)

                                                                                                                                                                                                                                     cGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg           >  1:777400/1‑239 (MQ=255)

                                                                                                                                                                                                                                       aaaGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGTTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGt          <  2:411796/238‑1 (MQ=255)

                                                                                                                                                                                                                                       aaaGTAATAACCAAAAGCAAGGAAGTAGGTTG‑CAGGGATTGTTGGTAAATTTCGCCGCCCTGATTTCTACCCGAAGGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTCCCATACGATGTTCGACAACATGCAAAAAGCGATTGCCCTGAATCGTCCTGCCTTTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg           <  1:903565/237‑1 (MQ=255)

                                                                                                                                                                                                                                                aGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTccc  >  1:212044/1‑237 (MQ=255)

ATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCC‑AGACAAAT‑ACAAAAACT‑GGAGTTGCCATGATG‑A‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCC  >  NC_000913/3206246‑3206716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`