Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,862,669 T→C 18.5% pseudogene (934/1617 nt) glvC ← pseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,862,6690TC18.5% 85.0 / 8.6 73pseudogene (934/1617 nt)glvCpseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component
Reads supporting (aligned to +/- strand):  ref base T (28/23);  new base C (2/10);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 2.41e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.92e-01

GATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGTTGCGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAAGTGG  >  NC_000913/3862433‑3862907
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                           
gATTTGGGGTAGCATCATATCCGCGTGGTTGCTGAACAACGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCGCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGTTGCGGGAATCa                                                                                                                                                                                                                                                          <  1:480124/239‑1 (MQ=255)
 aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGGATCa                                                                                                                                                                                                                                                          >  2:10248/1‑238 (MQ=255)
 aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGGATCa                                                                                                                                                                                                                                                          >  2:584744/1‑238 (MQ=255)
 aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAGGGTTCGGGCATCa                                                                                                                                                                                                                                                          >  1:1099592/1‑238 (MQ=255)
  tttGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTCAAGGTGCGGGAAACAgg                                                                                                                                                                                                                                                        >  2:671438/1‑238 (MQ=255)
  tttGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGTTGCGGGAATCAGc                                                                                                                                                                                                                                                        <  2:893081/239‑1 (MQ=255)
          aGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGAGTACCGCAAACAGCAACGGGGAAATGAAAAGGAAGGCAAATTCCAGAGGTTCGGTAATTCCCACCAGCATGGCGGTTAAAGGTGGTGGGATTAGCAAGtccc                                                                                                                                                                                                                                               >  1:29728/1‑237 (MQ=255)
                 tatCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGGTCGGGAATTCCCACCAGCATGGCGGTGTGGGTTGCGGGCATCAACCAGccccgacccttt                                                                                                                                                                                                                                       >  1:929592/1‑230 (MQ=255)
                       cgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAGGTTTCGGGAATCAGAAAGCCCGCCCCCTTTAccc                                                                                                                                                                                                                                >  2:143025/1‑237 (MQ=255)
                       cgTGGTTGCTGAACATCGGGATACAGTTTTGCGGCAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGAGTACCGCAAACAGCAACGGTGAAATGAAACGGAAGGTAAATTCCAGCGGTGCGGTAATTCCAACAAGCATGGCGGTTGAAGTTGTGGGAATAAGCAAGCCACGTCACTTTCAccgg                                                                                                                                                                                                                               >  1:326370/1‑237 (MQ=255)
                           gTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGGGTCAGCAAACCCCCCCCCTTTccccccgttt                                                                                                                                                                                                                          >  1:672052/1‑233 (MQ=255)
                              gCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATCCCCACCAGCATGGCGGTCACTAGTGAGGGAGCCCGTCACCCCCCCCCTCTTACCCGATTTTct                                                                                                                                                                                                                        >  1:779348/1‑237 (MQ=255)
                                 gAACAGCGGGACCCAGTTTTGCGGTAAAACCTAGTCAATCAGCCCCCCGCCCATGTTGCCCACCACACCAAAGAGATACATTAGGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGAAAACAGCAACGGTGAAATGAACAGGAAGGAAAATCCCAGCGGTTCGGTAATCCCCACCAGCATGGGGGTTAAGGTTGCGGGAATCAGAAAGCCCGCCACTTTTACCCGATTTTCCGGTg                                                                                                                                                                                                                    <  2:1024951/239‑1 (MQ=255)
                                              cAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGGATCAGCAAGACCCCCCCTTTTTCCCGATTTTTCCGTGGGGCCGGGAAGTa                                                                                                                                                                                                       >  2:414044/1‑239 (MQ=255)
                                                   ttGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTTAGGGTGGGGGGATCAGCAAGCCCCCCACTTTTAACCGATTTTCCGGTGGGGCGGGGAAGGACacc                                                                                                                                                                                                   >  2:1183473/1‑236 (MQ=255)
                                                      cGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGGAACAGCAAACCCCCCCATTTTACCCGATTTTTCGGGGCGGGAGGGAAGGACATCGCtc                                                                                                                                                                                              >  2:725604/1‑238 (MQ=255)
                                                           aaaCCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGAATCAGCCAGCCCGCCACATTTACCCGGTTTTTCGGGGCGGCAGTGAAAGAAAACGCCAAAGaa                                                                                                                                                                                        >  2:1179920/1‑239 (MQ=255)
                                                           aaaCCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAAGGTTCGGGGATAAGCAAACCCCCCCCCTTTTACCCATTTTTCCGGGCGGCACTGGAggaaaatgcctaagaa                                                                                                                                                                                          >  2:897927/1‑224 (MQ=255)
                                                                       tCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGGGGGAATCAACAAACCCCCCCCCTTTTACCCATTTTTCGGGGCGGCAGGGAAGGACAACCCTCAAAAAAAACCCACCGc                                                                                                                                                                           >  2:804903/1‑238 (MQ=255)
                                                                       tCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAAGTTGCGGGGATCAGCAAAACCGCCCCTTTTACCCGATTTTTCGGTGGGGCAGGGAAGGAAATCGCTAAAAAAAAGCCCACCGc                                                                                                                                                                           >  2:1100845/1‑238 (MQ=255)
                                                                        cAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGATTAAGGTGCGGGGATCAGCAAGGCCGCCACCTTTATCCGATTTTTCGGTGCGGCCGGGGAGGACAATCCTAAAGAAATGCCCACGGCa                                                                                                                                                                          >  2:730278/1‑238 (MQ=255)
                                                                                cGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGGGGGGAACAACAAAACCCCCCCTTTTAACCCCTTTTTCCGGGCGGCAGGGAAGGACATCCCCAAAAAAAACCCCCCGCGCACCAAAgtt                                                                                                                                                                  >  1:578228/1‑237 (MQ=255)
                                                                                         tGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTTAGGGTTGGGGGAACAGCAAGGCCCCCCCCTTTTCCCGGTTTTCCCGTGCGGGCGTGAAATACCTCCCTAAAGAAAAGCCCCCAGCACCCAAGATTTTTTAGtt                                                                                                                                                         >  2:992772/1‑238 (MQ=255)
                                                                                         tGCCCACAACACAAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTAAAATGAACAGGAAGGAAAATTCCAGCGGTTCGGTAATCCCCACCAGCAGGGCGGTTAAGGTTGCGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTa                                                                                                                                                       <  2:672052/239‑1 (MQ=255)
                                                                                          gCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTTAAGGTTCGGGAATCAGCAAACCCCCCCCTTTTACCCCATTTTTCCGGGCGGCCGTGAAATAAAACGCGAAAGAAAAACCCCCCGCCCCAAAGATTTTTGAGttt                                                                                                                                                        >  2:1133954/1‑237 (MQ=255)
                                                                                          gCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGGAATAGCCAACCCCCCCCCTTTTCCCCGTTTTTCGGTGGGGCGGGGAAGGACATCGCTAAAAAAAAGCCCACCGCCCCCAAAATTTTTTGGTTa                                                                                                                                                       >  1:494058/1‑238 (MQ=255)
                                                                                                          agaTACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTTAGGGTGCGGGGATCAGCAAGCCCCCCCCCTTTACCCCATTTTCCGGTGCGGCAGGGTAGAAAAACGCTAAAAAAAAGCCCCCCGCACCCAAGAGTTTTTAGTTTCCGTGCCGGGGaaaaa                                                                                                                                      >  1:1118046/1‑237 (MQ=255)
                                                                                                            aTACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTTAAGTTGCGGGGATCAGCAAAGCCCCCACCTTTTCCCCATTTTTCCGGGCGGCAGGTGAGTAAAACGCCCAAAAAAAACCCCACGCACCCAAAAATTTTTTGGTACCCTTGCAGGGAAAAAcc                                                                                                                                     >  2:692032/1‑238 (MQ=255)
                                                                                                             tACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGGATCAGCAAACCCGCCACTTTTTCCCGCTTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAAAAATGCCCCCGGCACCAAAGATTTTTTAGTTACCGTGCAGGGCAAAACCGc                                                                                                                                  >  2:1083222/1‑238 (MQ=255)
                                                                                                                  tACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTTAAGGTGTGGGGACCAGCCAAACCGCCACCTTTATCCGATTTTTCCGGGCGGCAGGGAAGTAAAACCCGCCAGAAAAACCCACGGCCCCCAAGGATTTTTGGGTTTCGGGCGGGGCAAAAcaccctctc                                                                                                                              >  1:1164301/1‑230 (MQ=255)
                                                                                                                    cGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAGGATGCGGGAATCAGCCAAACCGCCCCTTTTACCCCGATTTTCGGGGGGGCCGTGGAGGACAACGCTCAAAAAAAACCCACGGCCACAAAGGATTTTTAGTTTACGCGCCGGGCAAAAAAGCCCTCCcgg                                                                                                                          >  1:768516/1‑239 (MQ=255)
                                                                                                                     ggTAGACATGGAGGCCGCCAGCACGGCGTGTCCCGAAAACAGCAACGGTAAAATGAACGGGAAGGTAAATCCCAGCGGTTCGGTAATCCCCCCCGGCGTGGGGGTTAGGGTGGCGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCggg                                                                                                                          <  1:1100845/238‑1 (MQ=255)
                                                                                                                      gTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAAGTTGCGGGGAACAGCCAACCCCCCACCTTTTCCCCCTTTTTCCGTGCGGCAGGGGAGAACACACCTCAAAAAAAGCCCCCGGCACCAAAACATTTTGAGTTAACGTGCAGGGCAAAAACCCCTTCCcggt                                                                                                                         >  1:424778/1‑237 (MQ=255)
                                                                                                                         gACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAGGGTTCGGGAATCAGCAAACCCCCCCCTTTTTCCCGATTTTTCGGTGCGGCAGGGAAATACATCGCTAAAGAAAAGCCCACGGCCCCAAAGATTTTTTAGTTACCGTGCAGGGGAAAAACCCCCTCCGGGaaaaa                                                                                                                     >  2:65011/1‑239 (MQ=255)
                                                                                                                                     gccAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTTAGGGTGGGGGAAGCAACCAGCCCCCCACCTTTTACCCATTTTTCGGGGCGGCGGGGAAGTACACCGCCAAAGAAATTACCCCGGGCCCAAAGAATTTTTTTGTATCGGGCCGGGCGAAAACGACCTTCCGGGACAAAGAttttcaagg                                                                                                         >  1:15665/1‑233 (MQ=255)
                                                                                                                                     gccAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAAGGTTCGGGGATCAAGAAAGCCCCCCCTTTTTACCGATTTTTCGGTGCGGCGGGGAAGTAAATAGCTTAAAAAAATCCCCCGGCCCCCCAGAATTTTTAGTTAACCGGCCCGGGAAAAACGCCCTCCCGGGaaaaaggtctctacg                                                                                                           >  1:573412/1‑228 (MQ=255)
                                                                                                                                     gccAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAAGGTGCGGGGAACAAGAAGGCCCCCACCTTTTCCCGACTTTTCTGGGTGGCAGTGGAGGACAACGACTAAAAAAAACCCCCCGCCCCCAAGAAGTTTTTGTTACCGCGTCGGGGACAAACGCCCTCCCGGAACAACAACTTCAGCg                                                                                                          >  1:21287/1‑238 (MQ=255)
                                                                                                                                               cgggtcccGAAAACAGAAACGGTGAAATGAACAGGAGGGTAAATCCCAGCGGTGCGGAAATCCCCACCAGCAGGGCGGTTAGGGTGGGGGGAATAAGCAGGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCt                                                                                                <  2:1168160/232‑1 (MQ=255)
                                                                                                                                               cGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAGGGTGGGGGAAACACGAAACCCCCCCCTTTTTCCCCGTTTTTCCGTGCGGCAGTGAAGTAACACGCTAAAAAAAAGGCCCCCGCCCCCAAAGTTTTTTAGTTACCGGGCAGGGGAAAAACGCCCTTCCGGGACAACGATTTTCGCGCGTCGGGGCt                                                                                                >  1:640789/1‑238 (MQ=255)
                                                                                                                                                     cccaaaaCAGCAACGGTAAAAGGAACAGAAAGGAAAATTCCAGCGGTCCGTTAATCCCCACCAGCATGGCGGTTAGGGTGGCGGAAACCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTg                                                                                         <  2:599170/235‑1 (MQ=255)
                                                                                                                                                     ccGCAAACACACAGGGTAAAATAAACAGAAAGGAAAAGTCGAGCGGTTCGGTAACTCCCCCCGGCCGGGCGGTAAGGGGTGGGGAAACCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTg                                                                                         <  1:1168003/239‑1 (MQ=255)
                                                                                                                                                          aacaagaaagggtaaaataaaaGGAAAGTAAAATCCCAGCGGTTCGTAAATCCCCCCAGGCATGGGGGTTAAGGTGGCGGAAACCAGCAACCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAAct                                                                                     <  2:578228/218‑1 (MQ=255)
                                                                                                                                                               cAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTTCGGGAATCAAAGAGCCCGGCACTTTTACCCGGTTTTCCGGTGCGGCAGGGGAGGACAACCCTCAAGAAATGCCCCCGGCCCCCAAGAATTTTGTGGTACCGTGCCGGGCCAAAACGCCCTCCCGGGACAAAGATTTTAGCGGGTCGGCGCGCACACtagaccacctg                                                                                >  1:615608/1‑228 (MQ=255)
                                                                                                                                                                aggggTAAAATAAAGAGAAAGGAAAATTCCAGCGGTCGGTTAATCCCCACCAGCATGGCGGTTAAGGTGGGGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAg                                                                              <  2:853302/236‑1 (MQ=255)
                                                                                                                                                                    gTGAAATGAACAGAAAGGAAAATTCCAGCGTTTCGGTAATCCCCACCAGCAGGGCGGTTAAGGTTGCGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGAtg                                                                           <  2:1118046/238‑1 (MQ=255)
                                                                                                                                                                      aaaaTGAACAGGAAGGAAAATTCCGGCGTGCCGGAAATCCCCACCAGCATGGTGTTTAGGGTGGCGGGAACCAACAACCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGAtgct                                                                         <  1:804903/237‑1 (MQ=255)
                                                                                                                                                                        aaagAACAGGAAGGTAAATCCCGGCGGTCCGATAATCCCCACCAACAGGGCTGTTAAGGTTGGGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTgcg                                                                      <  2:15665/236‑1 (MQ=255)
                                                                                                                                                                        aaTGAAGAGAAGGGTAAATCCCGGGGGTTGGGTAATCCCCCCCGGCATGCGGTGTAAGTGGGCGGAAACCAGAAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTgcg                                                                      <  2:745465/239‑1 (MQ=255)
                                                                                                                                                                        aaTGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCCCCAGCAGGGCGGTTAAGGTGGCGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTgcg                                                                      <  2:471051/239‑1 (MQ=255)
                                                                                                                                                                          taaaCAGGAAGGAAAATCCCAGCGGTTCGATAACCCCCACCAGCATGGCGTTTAAGGTGGGGGGAACCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc                                                                    <  2:1099592/237‑1 (MQ=255)
                                                                                                                                                                          tGAACAGCAAGGTAAATACCAGCGGTTCGTTAATTCCCACCAGCATGGCGGTTTAGGTTACGGGAATCAACAAGCCCGCCACCTTTACCCGATTTTCCGGTGCGGCAGTGAAGTGCATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc                                                                    <  2:876063/239‑1 (MQ=255)
                                                                                                                                                                          tGAACAGAAAGGAAAATCCCAGCGTGGCGTTAATCCCCCCCAGCTTGTGGTTTAGGGTGGCGGGAATCAGCAAGGCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc                                                                    <  2:929592/239‑1 (MQ=255)
                                                                                                                                                                          agAACAGTAAGTTAAACCCCAGTGGTGTGATATCCCCCCCCAGCATGGCGTTTAGGGGTGCGGAAACCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc                                                                    <  1:725604/238‑1 (MQ=255)
                                                                                                                                                                           tgaaaaggagggaaatgtcgggCGGTTGGGAAATTCCCACCAGCATGGCGGTTAAGGGGGCGGGAAACAGCAGGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc                                                                    <  2:655189/218‑1 (MQ=255)
                                                                                                                                                                               aGGAAGTTAAATTCCGGCGGTTTGGTAATCCCCACCAGGATGGCGTTTGTGGTTGCGGAAATCATCATGACCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGTGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTa                                                               <  2:144653/239‑1 (MQ=255)
                                                                                                                                                                                gaaaGGTAAATCCCGGCGGTCCGTAAACCCCCCCCAGCGTGGGGTTTAGGGTGGCGGAAACCAAAAACCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCGCGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTa                                                               <  1:16444/236‑1 (MQ=255)
                                                                                                                                                                                            tccgggggtcggttAATCCCCCCCAGGTGGGGGGTTAAGGTGGGGGGAATCAGAACGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATgcc                                                   <  1:1083222/226‑1 (MQ=255)
                                                                                                                                                                                            tCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGTTGCGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGGGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCGCGGCACCAAAGATTTTTTAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGGACAAAGATTTTAGCGGCTCGGGGCCCAAAATGAACTCTTGCAGATGCTGCGCCCAGGACATCTGAATgc                                                    >  2:16444/1‑238 (MQ=255)
                                                                                                                                                                                                gCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAGGGTGCGGGGATCAGCAAGCCCCCCCCCTTTACCCGGTTTTCCGGTGCGGCAGGGAAGTACATCGCTTAAAAAAATCCCCCGGCCCCAAAGATTTTTTAGTTTCCGTGGAAGGCAAAACCGCCTTCCGGGAACAAAGATTTCAGCGGATCGGGGCTCCAAACGGACTCTTTCCCAAGCTGCcccccacacacacggcaggccg                                                 >  1:951874/1‑219 (MQ=255)
                                                                                                                                                                                                           atccccACCAGCATGGCGGTTAAGGTTGCGGGAACAAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGc                                    <  2:615608/236‑1 (MQ=255)
                                                                                                                                                                                                                  gcacagcaggtgggTTAAGGTGGCGGAACAAAGCAACCCCGCCACCTTTACCCGATTGTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGAc                               <  1:803234/227‑1 (MQ=255)
                                                                                                                                                                                                                  ccaGCTGGGCGGTTAGGGTGGCGGGAACCAGCACGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCa                             <  1:992772/239‑1 (MQ=255)
                                                                                                                                                                                                                   cgcgaaTGGCGTTAAGGGTCGCGGAAATAAGAACGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCa                             <  2:327359/234‑1 (MQ=255)
                                                                                                                                                                                                                   ccacaagggCGGTAAGGGGTGCGGAAATCAGAAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCa                             <  2:549684/232‑1 (MQ=255)
                                                                                                                                                                                                                       cATGGCGGTTAAGGTTGCGGGAATCAGCAAGCCCGCCCCCTTTTCCCGATTTTCCGGTGCGGCAGGGAAGGACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTTAGTTACCGTGCAGGGCAAAAACCCCCTCCGGGAAAAACGATTTCAGCGGCTCGGCGCGCCAACCGAAACCTTTACGATTGTGCGCCCAGTGCACCCGAAAgccccccttcaccccttgcgggcccaaag                         >  1:68902/1‑216 (MQ=255)
                                                                                                                                                                                                                            cGGTTAAGGTTGGGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATaaa                     <  2:1216924/238‑1 (MQ=255)
                                                                                                                                                                                                                               cgttatggtgggCGGTAATCAGCCACCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATaaa                     <  1:1179920/227‑1 (MQ=255)
                                                                                                                                                                                                                                 gtaaggtgggCGGAAATAACCAACCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACt                   <  2:687554/229‑1 (MQ=255)
                                                                                                                                                                                                                                   tAAGGTTGGGGAATTCACAAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCATTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTc                <  2:520416/239‑1 (MQ=255)
                                                                                                                                                                                                                                    agggTTGCGGAAATCAGCAGGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCAGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTcc               <  2:743909/237‑1 (MQ=255)
                                                                                                                                                                                                                                    agggTTGCGGAAATCAGCAACCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTcc               <  2:640789/237‑1 (MQ=255)
                                                                                                                                                                                                                                       gTTCCGGAAATCAGCAAGCCCGCCACCTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGt             <  2:664855/238‑1 (MQ=255)
                                                                                                                                                                                                                                           cGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGGACATATGAATGCCGCCTTCAACCGCTGCCGGACCAAAGATAACCTGTCCGTAgtcg        >  1:735657/1‑236 (MQ=255)
                                                                                                                                                                                                                                                  tCAGCAAGACCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGTGCTCAAACTGAACTCTTGCAGATGATGCGCCCAGTACATTTGAAAGCCCCCTTCCACAGCTGCCGGACCAAAGCTACATTGGCCGTAGagtaagggg  >  1:775094/1‑231 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                           
GATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGTTGCGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAAGTGG  >  NC_000913/3862433‑3862907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: