Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,862,672 G→A 14.2% pseudogene (931/1617 nt) glvC ← pseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,862,6720GA14.2% 92.7 / 3.4 70pseudogene (931/1617 nt)glvCpseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component
Reads supporting (aligned to +/- strand):  ref base G (28/28);  new base A (6/3);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.17e-01

TTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGTTGCGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAAGTGGT  >  NC_000913/3862435‑3862908
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                        
tttGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTCAAGGTGCGGGAAACAgg                                                                                                                                                                                                                                                         >  2:671438/1‑238 (MQ=255)
tttGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGTTGCGGGAATCAGc                                                                                                                                                                                                                                                         <  2:893081/239‑1 (MQ=255)
        aGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGAGTACCGCAAACAGCAACGGGGAAATGAAAAGGAAGGCAAATTCCAGAGGTTCGGTAATTCCCACCAGCATGGCGGTTAAAGGTGGTGGGATTAGCAAGtccc                                                                                                                                                                                                                                                >  1:29728/1‑237 (MQ=255)
               tatCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGGTCGGGAATTCCCACCAGCATGGCGGTGTGGGTTGCGGGCATCAACCAGccccgacccttt                                                                                                                                                                                                                                        >  1:929592/1‑230 (MQ=255)
                     cgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAGGTTTCGGGAATCAGAAAGCCCGCCCCCTTTAccc                                                                                                                                                                                                                                 >  2:143025/1‑237 (MQ=255)
                     cgTGGTTGCTGAACATCGGGATACAGTTTTGCGGCAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGAGTACCGCAAACAGCAACGGTGAAATGAAACGGAAGGTAAATTCCAGCGGTGCGGTAATTCCAACAAGCATGGCGGTTGAAGTTGTGGGAATAAGCAAGCCACGTCACTTTCAccgg                                                                                                                                                                                                                                >  1:326370/1‑237 (MQ=255)
                         gTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGGGTCAGCAAACCCCCCCCCTTTccccccgttt                                                                                                                                                                                                                           >  1:672052/1‑233 (MQ=255)
                            gCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATCCCCACCAGCATGGCGGTCACTAGTGAGGGAGCCCGTCACCCCCCCCCTCTTACCCGATTTTct                                                                                                                                                                                                                         >  1:779348/1‑237 (MQ=255)
                               gAACAGCGGGACCCAGTTTTGCGGTAAAACCTAGTCAATCAGCCCCCCGCCCATGTTGCCCACCACACCAAAGAGATACATTAGGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGAAAACAGCAACGGTGAAATGAACAGGAAGGAAAATCCCAGCGGTTCGGTAATCCCCACCAGCATGGGGGTTAAGGTTGCGGGAATCAGAAAGCCCGCCACTTTTACCCGATTTTCCGGTg                                                                                                                                                                                                                     <  2:1024951/239‑1 (MQ=255)
                                            cAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGGATCAGCAAGACCCCCCCTTTTTCCCGATTTTTCCGTGGGGCCGGGAAGTa                                                                                                                                                                                                        >  2:414044/1‑239 (MQ=255)
                                                 ttGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTTAGGGTGGGGGGATCAGCAAGCCCCCCACTTTTAACCGATTTTCCGGTGGGGCGGGGAAGGACacc                                                                                                                                                                                                    >  2:1183473/1‑236 (MQ=255)
                                                    cGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGGAACAGCAAACCCCCCCATTTTACCCGATTTTTCGGGGCGGGAGGGAAGGACATCGCtc                                                                                                                                                                                               >  2:725604/1‑238 (MQ=255)
                                                         aaaCCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGAATCAGCCAGCCCGCCACATTTACCCGGTTTTTCGGGGCGGCAGTGAAAGAAAACGCCAAAGaa                                                                                                                                                                                         >  2:1179920/1‑239 (MQ=255)
                                                         aaaCCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAAGGTTCGGGGATAAGCAAACCCCCCCCCTTTTACCCATTTTTCCGGGCGGCACTGGAggaaaatgcctaagaa                                                                                                                                                                                           >  2:897927/1‑224 (MQ=255)
                                                                     tCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGGGGGAATCAACAAACCCCCCCCCTTTTACCCATTTTTCGGGGCGGCAGGGAAGGACAACCCTCAAAAAAAACCCACCGc                                                                                                                                                                            >  2:804903/1‑238 (MQ=255)
                                                                     tCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAAGTTGCGGGGATCAGCAAAACCGCCCCTTTTACCCGATTTTTCGGTGGGGCAGGGAAGGAAATCGCTAAAAAAAAGCCCACCGc                                                                                                                                                                            >  2:1100845/1‑238 (MQ=255)
                                                                      cAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGATTAAGGTGCGGGGATCAGCAAGGCCGCCACCTTTATCCGATTTTTCGGTGCGGCCGGGGAGGACAATCCTAAAGAAATGCCCACGGCa                                                                                                                                                                           >  2:730278/1‑238 (MQ=255)
                                                                              cGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGGGGGGAACAACAAAACCCCCCCTTTTAACCCCTTTTTCCGGGCGGCAGGGAAGGACATCCCCAAAAAAAACCCCCCGCGCACCAAAgtt                                                                                                                                                                   >  1:578228/1‑237 (MQ=255)
                                                                                       tGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTTAGGGTTGGGGGAACAGCAAGGCCCCCCCCTTTTCCCGGTTTTCCCGTGCGGGCGTGAAATACCTCCCTAAAGAAAAGCCCCCAGCACCCAAGATTTTTTAGtt                                                                                                                                                          >  2:992772/1‑238 (MQ=255)
                                                                                       tGCCCACAACACAAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTAAAATGAACAGGAAGGAAAATTCCAGCGGTTCGGTAATCCCCACCAGCAGGGCGGTTAAGGTTGCGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTa                                                                                                                                                        <  2:672052/239‑1 (MQ=255)
                                                                                        gCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTTAAGGTTCGGGAATCAGCAAACCCCCCCCTTTTACCCCATTTTTCCGGGCGGCCGTGAAATAAAACGCGAAAGAAAAACCCCCCGCCCCAAAGATTTTTGAGttt                                                                                                                                                         >  2:1133954/1‑237 (MQ=255)
                                                                                        gCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGGAATAGCCAACCCCCCCCCTTTTCCCCGTTTTTCGGTGGGGCGGGGAAGGACATCGCTAAAAAAAAGCCCACCGCCCCCAAAATTTTTTGGTTa                                                                                                                                                        >  1:494058/1‑238 (MQ=255)
                                                                                                        agaTACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTTAGGGTGCGGGGATCAGCAAGCCCCCCCCCTTTACCCCATTTTCCGGTGCGGCAGGGTAGAAAAACGCTAAAAAAAAGCCCCCCGCACCCAAGAGTTTTTAGTTTCCGTGCCGGGGaaaaa                                                                                                                                       >  1:1118046/1‑237 (MQ=255)
                                                                                                          aTACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTTAAGTTGCGGGGATCAGCAAAGCCCCCACCTTTTCCCCATTTTTCCGGGCGGCAGGTGAGTAAAACGCCCAAAAAAAACCCCACGCACCCAAAAATTTTTTGGTACCCTTGCAGGGAAAAAcc                                                                                                                                      >  2:692032/1‑238 (MQ=255)
                                                                                                           tACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGGATCAGCAAACCCGCCACTTTTTCCCGCTTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAAAAATGCCCCCGGCACCAAAGATTTTTTAGTTACCGTGCAGGGCAAAACCGc                                                                                                                                   >  2:1083222/1‑238 (MQ=255)
                                                                                                                tACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTTAAGGTGTGGGGACCAGCCAAACCGCCACCTTTATCCGATTTTTCCGGGCGGCAGGGAAGTAAAACCCGCCAGAAAAACCCACGGCCCCCAAGGATTTTTGGGTTTCGGGCGGGGCAAAAcaccctctc                                                                                                                               >  1:1164301/1‑230 (MQ=255)
                                                                                                                  cGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAGGATGCGGGAATCAGCCAAACCGCCCCTTTTACCCCGATTTTCGGGGGGGCCGTGGAGGACAACGCTCAAAAAAAACCCACGGCCACAAAGGATTTTTAGTTTACGCGCCGGGCAAAAAAGCCCTCCcgg                                                                                                                           >  1:768516/1‑239 (MQ=255)
                                                                                                                   ggTAGACATGGAGGCCGCCAGCACGGCGTGTCCCGAAAACAGCAACGGTAAAATGAACGGGAAGGTAAATCCCAGCGGTTCGGTAATCCCCCCCGGCGTGGGGGTTAGGGTGGCGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCggg                                                                                                                           <  1:1100845/238‑1 (MQ=255)
                                                                                                                    gTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAAGTTGCGGGGAACAGCCAACCCCCCACCTTTTCCCCCTTTTTCCGTGCGGCAGGGGAGAACACACCTCAAAAAAAGCCCCCGGCACCAAAACATTTTGAGTTAACGTGCAGGGCAAAAACCCCTTCCcggt                                                                                                                          >  1:424778/1‑237 (MQ=255)
                                                                                                                       gACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAGGGTTCGGGAATCAGCAAACCCCCCCCTTTTTCCCGATTTTTCGGTGCGGCAGGGAAATACATCGCTAAAGAAAAGCCCACGGCCCCAAAGATTTTTTAGTTACCGTGCAGGGGAAAAACCCCCTCCGGGaaaaa                                                                                                                      >  2:65011/1‑239 (MQ=255)
                                                                                                                                   gccAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTTAGGGTGGGGGAAGCAACCAGCCCCCCACCTTTTACCCATTTTTCGGGGCGGCGGGGAAGTACACCGCCAAAGAAATTACCCCGGGCCCAAAGAATTTTTTTGTATCGGGCCGGGCGAAAACGACCTTCCGGGACAAAGAttttcaagg                                                                                                          >  1:15665/1‑233 (MQ=255)
                                                                                                                                   gccAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAAGGTTCGGGGATCAAGAAAGCCCCCCCTTTTTACCGATTTTTCGGTGCGGCGGGGAAGTAAATAGCTTAAAAAAATCCCCCGGCCCCCCAGAATTTTTAGTTAACCGGCCCGGGAAAAACGCCCTCCCGGGaaaaaggtctctacg                                                                                                            >  1:573412/1‑228 (MQ=255)
                                                                                                                                   gccAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAAGGTGCGGGGAACAAGAAGGCCCCCACCTTTTCCCGACTTTTCTGGGTGGCAGTGGAGGACAACGACTAAAAAAAACCCCCCGCCCCCAAGAAGTTTTTGTTACCGCGTCGGGGACAAACGCCCTCCCGGAACAACAACTTCAGCg                                                                                                           >  1:21287/1‑238 (MQ=255)
                                                                                                                                             cgggtcccGAAAACAGAAACGGTGAAATGAACAGGAGGGTAAATCCCAGCGGTGCGGAAATCCCCACCAGCAGGGCGGTTAGGGTGGGGGGAATAAGCAGGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCt                                                                                                 <  2:1168160/232‑1 (MQ=255)
                                                                                                                                             cGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAGGGTGGGGGAAACACGAAACCCCCCCCTTTTTCCCCGTTTTTCCGTGCGGCAGTGAAGTAACACGCTAAAAAAAAGGCCCCCGCCCCCAAAGTTTTTTAGTTACCGGGCAGGGGAAAAACGCCCTTCCGGGACAACGATTTTCGCGCGTCGGGGCt                                                                                                 >  1:640789/1‑238 (MQ=255)
                                                                                                                                                   cccaaaaCAGCAACGGTAAAAGGAACAGAAAGGAAAATTCCAGCGGTCCGTTAATCCCCACCAGCATGGCGGTTAGGGTGGCGGAAACCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTg                                                                                          <  2:599170/235‑1 (MQ=255)
                                                                                                                                                   ccGCAAACACACAGGGTAAAATAAACAGAAAGGAAAAGTCGAGCGGTTCGGTAACTCCCCCCGGCCGGGCGGTAAGGGGTGGGGAAACCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTg                                                                                          <  1:1168003/239‑1 (MQ=255)
                                                                                                                                                        aacaagaaagggtaaaataaaaGGAAAGTAAAATCCCAGCGGTTCGTAAATCCCCCCAGGCATGGGGGTTAAGGTGGCGGAAACCAGCAACCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAAct                                                                                      <  2:578228/218‑1 (MQ=255)
                                                                                                                                                             cAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTTCGGGAATCAAAGAGCCCGGCACTTTTACCCGGTTTTCCGGTGCGGCAGGGGAGGACAACCCTCAAGAAATGCCCCCGGCCCCCAAGAATTTTGTGGTACCGTGCCGGGCCAAAACGCCCTCCCGGGACAAAGATTTTAGCGGGTCGGCGCGCACACtagaccacctg                                                                                 >  1:615608/1‑228 (MQ=255)
                                                                                                                                                              aggggTAAAATAAAGAGAAAGGAAAATTCCAGCGGTCGGTTAATCCCCACCAGCATGGCGGTTAAGGTGGGGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAg                                                                               <  2:853302/236‑1 (MQ=255)
                                                                                                                                                                  gTGAAATGAACAGAAAGGAAAATTCCAGCGTTTCGGTAATCCCCACCAGCAGGGCGGTTAAGGTTGCGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGAtg                                                                            <  2:1118046/238‑1 (MQ=255)
                                                                                                                                                                    aaaaTGAACAGGAAGGAAAATTCCGGCGTGCCGGAAATCCCCACCAGCATGGTGTTTAGGGTGGCGGGAACCAACAACCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGAtgct                                                                          <  1:804903/237‑1 (MQ=255)
                                                                                                                                                                      aaagAACAGGAAGGTAAATCCCGGCGGTCCGATAATCCCCACCAACAGGGCTGTTAAGGTTGGGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTgcg                                                                       <  2:15665/236‑1 (MQ=255)
                                                                                                                                                                      aaTGAAGAGAAGGGTAAATCCCGGGGGTTGGGTAATCCCCCCCGGCATGCGGTGTAAGTGGGCGGAAACCAGAAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTgcg                                                                       <  2:745465/239‑1 (MQ=255)
                                                                                                                                                                      aaTGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCCCCAGCAGGGCGGTTAAGGTGGCGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTgcg                                                                       <  2:471051/239‑1 (MQ=255)
                                                                                                                                                                        taaaCAGGAAGGAAAATCCCAGCGGTTCGATAACCCCCACCAGCATGGCGTTTAAGGTGGGGGGAACCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc                                                                     <  2:1099592/237‑1 (MQ=255)
                                                                                                                                                                        tGAACAGCAAGGTAAATACCAGCGGTTCGTTAATTCCCACCAGCATGGCGGTTTAGGTTACGGGAATCAACAAGCCCGCCACCTTTACCCGATTTTCCGGTGCGGCAGTGAAGTGCATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc                                                                     <  2:876063/239‑1 (MQ=255)
                                                                                                                                                                        tGAACAGAAAGGAAAATCCCAGCGTGGCGTTAATCCCCCCCAGCTTGTGGTTTAGGGTGGCGGGAATCAGCAAGGCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc                                                                     <  2:929592/239‑1 (MQ=255)
                                                                                                                                                                        agAACAGTAAGTTAAACCCCAGTGGTGTGATATCCCCCCCCAGCATGGCGTTTAGGGGTGCGGAAACCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc                                                                     <  1:725604/238‑1 (MQ=255)
                                                                                                                                                                         tgaaaaggagggaaatgtcgggCGGTTGGGAAATTCCCACCAGCATGGCGGTTAAGGGGGCGGGAAACAGCAGGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc                                                                     <  2:655189/218‑1 (MQ=255)
                                                                                                                                                                             aGGAAGTTAAATTCCGGCGGTTTGGTAATCCCCACCAGGATGGCGTTTGTGGTTGCGGAAATCATCATGACCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGTGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTa                                                                <  2:144653/239‑1 (MQ=255)
                                                                                                                                                                              gaaaGGTAAATCCCGGCGGTCCGTAAACCCCCCCCAGCGTGGGGTTTAGGGTGGCGGAAACCAAAAACCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCGCGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTa                                                                <  1:16444/236‑1 (MQ=255)
                                                                                                                                                                                          tccgggggtcggttAATCCCCCCCAGGTGGGGGGTTAAGGTGGGGGGAATCAGAACGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATgcc                                                    <  1:1083222/226‑1 (MQ=255)
                                                                                                                                                                                          tCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGTTGCGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGGGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCGCGGCACCAAAGATTTTTTAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGGACAAAGATTTTAGCGGCTCGGGGCCCAAAATGAACTCTTGCAGATGCTGCGCCCAGGACATCTGAATgc                                                     >  2:16444/1‑238 (MQ=255)
                                                                                                                                                                                              gCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAGGGTGCGGGGATCAGCAAGCCCCCCCCCTTTACCCGGTTTTCCGGTGCGGCAGGGAAGTACATCGCTTAAAAAAATCCCCCGGCCCCAAAGATTTTTTAGTTTCCGTGGAAGGCAAAACCGCCTTCCGGGAACAAAGATTTCAGCGGATCGGGGCTCCAAACGGACTCTTTCCCAAGCTGCcccccacacacacggcaggccg                                                  >  1:951874/1‑219 (MQ=255)
                                                                                                                                                                                                         atccccACCAGCATGGCGGTTAAGGTTGCGGGAACAAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGc                                     <  2:615608/236‑1 (MQ=255)
                                                                                                                                                                                                                gcacagcaggtgggTTAAGGTGGCGGAACAAAGCAACCCCGCCACCTTTACCCGATTGTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGAc                                <  1:803234/227‑1 (MQ=255)
                                                                                                                                                                                                                ccaGCTGGGCGGTTAGGGTGGCGGGAACCAGCACGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCa                              <  1:992772/239‑1 (MQ=255)
                                                                                                                                                                                                                 cgcgaaTGGCGTTAAGGGTCGCGGAAATAAGAACGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCa                              <  2:327359/234‑1 (MQ=255)
                                                                                                                                                                                                                 ccacaagggCGGTAAGGGGTGCGGAAATCAGAAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCa                              <  2:549684/232‑1 (MQ=255)
                                                                                                                                                                                                                     cATGGCGGTTAAGGTTGCGGGAATCAGCAAGCCCGCCCCCTTTTCCCGATTTTCCGGTGCGGCAGGGAAGGACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTTAGTTACCGTGCAGGGCAAAAACCCCCTCCGGGAAAAACGATTTCAGCGGCTCGGCGCGCCAACCGAAACCTTTACGATTGTGCGCCCAGTGCACCCGAAAgccccccttcaccccttgcgggcccaaag                          >  1:68902/1‑216 (MQ=255)
                                                                                                                                                                                                                          cGGTTAAGGTTGGGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATaaa                      <  2:1216924/238‑1 (MQ=255)
                                                                                                                                                                                                                             cgttatggtgggCGGTAATCAGCCACCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATaaa                      <  1:1179920/227‑1 (MQ=255)
                                                                                                                                                                                                                               gtaaggtgggCGGAAATAACCAACCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACt                    <  2:687554/229‑1 (MQ=255)
                                                                                                                                                                                                                                 tAAGGTTGGGGAATTCACAAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCATTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTc                 <  2:520416/239‑1 (MQ=255)
                                                                                                                                                                                                                                  agggTTGCGGAAATCAGCAGGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCAGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTcc                <  2:743909/237‑1 (MQ=255)
                                                                                                                                                                                                                                  agggTTGCGGAAATCAGCAACCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTcc                <  2:640789/237‑1 (MQ=255)
                                                                                                                                                                                                                                     gTTCCGGAAATCAGCAAGCCCGCCACCTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGt              <  2:664855/238‑1 (MQ=255)
                                                                                                                                                                                                                                         cGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGGACATATGAATGCCGCCTTCAACCGCTGCCGGACCAAAGATAACCTGTCCGTAgtcg         >  1:735657/1‑236 (MQ=255)
                                                                                                                                                                                                                                                tCAGCAAGACCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGTGCTCAAACTGAACTCTTGCAGATGATGCGCCCAGTACATTTGAAAGCCCCCTTCCACAGCTGCCGGACCAAAGCTACATTGGCCGTAGagtaagggg   >  1:775094/1‑231 (MQ=255)
                                                                                                                                                                                                                                                 cAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCACTACATCTGAATGCCGCATTCAACAGCTGCCAGTCCAAAGATAAACTGGCCGTCGATGaatcggt  >  1:689575/1‑234 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                        
TTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGTTGCGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAAGTGGT  >  NC_000913/3862435‑3862908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: