Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,862,685 T→C 17.4% pseudogene (918/1617 nt) glvC ← pseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,862,6850TC17.4% 166.5 / 6.7 74pseudogene (918/1617 nt)glvCpseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component
Reads supporting (aligned to +/- strand):  ref base T (25/34);  new base C (11/3);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.86e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGTTGCGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAAGTGGTGTAAACCGGTT  >  NC_000913/3862456‑3862919
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   
cgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAGGTTTCGGGAATCAGAAAGCCCGCCCCCTTTAccc                                                                                                                                                                                                                                            >  2:143025/1‑237 (MQ=255)
cgTGGTTGCTGAACATCGGGATACAGTTTTGCGGCAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGAGTACCGCAAACAGCAACGGTGAAATGAAACGGAAGGTAAATTCCAGCGGTGCGGTAATTCCAACAAGCATGGCGGTTGAAGTTGTGGGAATAAGCAAGCCACGTCACTTTCAccgg                                                                                                                                                                                                                                           >  1:326370/1‑237 (MQ=255)
    gTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGGGTCAGCAAACCCCCCCCCTTTccccccgttt                                                                                                                                                                                                                                      >  1:672052/1‑233 (MQ=255)
       gCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATCCCCACCAGCATGGCGGTCACTAGTGAGGGAGCCCGTCACCCCCCCCCTCTTACCCGATTTTct                                                                                                                                                                                                                                    >  1:779348/1‑237 (MQ=255)
          gAACAGCGGGACCCAGTTTTGCGGTAAAACCTAGTCAATCAGCCCCCCGCCCATGTTGCCCACCACACCAAAGAGATACATTAGGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGAAAACAGCAACGGTGAAATGAACAGGAAGGAAAATCCCAGCGGTTCGGTAATCCCCACCAGCATGGGGGTTAAGGTTGCGGGAATCAGAAAGCCCGCCACTTTTACCCGATTTTCCGGTg                                                                                                                                                                                                                                <  2:1024951/239‑1 (MQ=255)
                       cAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGGATCAGCAAGACCCCCCCTTTTTCCCGATTTTTCCGTGGGGCCGGGAAGTa                                                                                                                                                                                                                   >  2:414044/1‑239 (MQ=255)
                            ttGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTTAGGGTGGGGGGATCAGCAAGCCCCCCACTTTTAACCGATTTTCCGGTGGGGCGGGGAAGGACacc                                                                                                                                                                                                               >  2:1183473/1‑236 (MQ=255)
                               cGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGGAACAGCAAACCCCCCCATTTTACCCGATTTTTCGGGGCGGGAGGGAAGGACATCGCtc                                                                                                                                                                                                          >  2:725604/1‑238 (MQ=255)
                                    aaaCCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGAATCAGCCAGCCCGCCACATTTACCCGGTTTTTCGGGGCGGCAGTGAAAGAAAACGCCAAAGaa                                                                                                                                                                                                    >  2:1179920/1‑239 (MQ=255)
                                    aaaCCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAAGGTTCGGGGATAAGCAAACCCCCCCCCTTTTACCCATTTTTCCGGGCGGCACTGGAggaaaatgcctaagaa                                                                                                                                                                                                      >  2:897927/1‑224 (MQ=255)
                                                tCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGGGGGAATCAACAAACCCCCCCCCTTTTACCCATTTTTCGGGGCGGCAGGGAAGGACAACCCTCAAAAAAAACCCACCGc                                                                                                                                                                                       >  2:804903/1‑238 (MQ=255)
                                                tCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAAGTTGCGGGGATCAGCAAAACCGCCCCTTTTACCCGATTTTTCGGTGGGGCAGGGAAGGAAATCGCTAAAAAAAAGCCCACCGc                                                                                                                                                                                       >  2:1100845/1‑238 (MQ=255)
                                                 cAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGATTAAGGTGCGGGGATCAGCAAGGCCGCCACCTTTATCCGATTTTTCGGTGCGGCCGGGGAGGACAATCCTAAAGAAATGCCCACGGCa                                                                                                                                                                                      >  2:730278/1‑238 (MQ=255)
                                                         cGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGGGGGGAACAACAAAACCCCCCCTTTTAACCCCTTTTTCCGGGCGGCAGGGAAGGACATCCCCAAAAAAAACCCCCCGCGCACCAAAgtt                                                                                                                                                                              >  1:578228/1‑237 (MQ=255)
                                                                  tGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTTAGGGTTGGGGGAACAGCAAGGCCCCCCCCTTTTCCCGGTTTTCCCGTGCGGGCGTGAAATACCTCCCTAAAGAAAAGCCCCCAGCACCCAAGATTTTTTAGtt                                                                                                                                                                     >  2:992772/1‑238 (MQ=255)
                                                                  tGCCCACAACACAAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTAAAATGAACAGGAAGGAAAATTCCAGCGGTTCGGTAATCCCCACCAGCAGGGCGGTTAAGGTTGCGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTa                                                                                                                                                                   <  2:672052/239‑1 (MQ=255)
                                                                   gCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTTAAGGTTCGGGAATCAGCAAACCCCCCCCTTTTACCCCATTTTTCCGGGCGGCCGTGAAATAAAACGCGAAAGAAAAACCCCCCGCCCCAAAGATTTTTGAGttt                                                                                                                                                                    >  2:1133954/1‑237 (MQ=255)
                                                                   gCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGGAATAGCCAACCCCCCCCCTTTTCCCCGTTTTTCGGTGGGGCGGGGAAGGACATCGCTAAAAAAAAGCCCACCGCCCCCAAAATTTTTTGGTTa                                                                                                                                                                   >  1:494058/1‑238 (MQ=255)
                                                                                   agaTACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTTAGGGTGCGGGGATCAGCAAGCCCCCCCCCTTTACCCCATTTTCCGGTGCGGCAGGGTAGAAAAACGCTAAAAAAAAGCCCCCCGCACCCAAGAGTTTTTAGTTTCCGTGCCGGGGaaaaa                                                                                                                                                  >  1:1118046/1‑237 (MQ=255)
                                                                                     aTACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTTAAGTTGCGGGGATCAGCAAAGCCCCCACCTTTTCCCCATTTTTCCGGGCGGCAGGTGAGTAAAACGCCCAAAAAAAACCCCACGCACCCAAAAATTTTTTGGTACCCTTGCAGGGAAAAAcc                                                                                                                                                 >  2:692032/1‑238 (MQ=255)
                                                                                      tACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTGCGGGGATCAGCAAACCCGCCACTTTTTCCCGCTTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAAAAATGCCCCCGGCACCAAAGATTTTTTAGTTACCGTGCAGGGCAAAACCGc                                                                                                                                              >  2:1083222/1‑238 (MQ=255)
                                                                                           tACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTTAAGGTGTGGGGACCAGCCAAACCGCCACCTTTATCCGATTTTTCCGGGCGGCAGGGAAGTAAAACCCGCCAGAAAAACCCACGGCCCCCAAGGATTTTTGGGTTTCGGGCGGGGCAAAAcaccctctc                                                                                                                                          >  1:1164301/1‑230 (MQ=255)
                                                                                             cGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAGGATGCGGGAATCAGCCAAACCGCCCCTTTTACCCCGATTTTCGGGGGGGCCGTGGAGGACAACGCTCAAAAAAAACCCACGGCCACAAAGGATTTTTAGTTTACGCGCCGGGCAAAAAAGCCCTCCcgg                                                                                                                                      >  1:768516/1‑239 (MQ=255)
                                                                                              ggTAGACATGGAGGCCGCCAGCACGGCGTGTCCCGAAAACAGCAACGGTAAAATGAACGGGAAGGTAAATCCCAGCGGTTCGGTAATCCCCCCCGGCGTGGGGGTTAGGGTGGCGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCggg                                                                                                                                      <  1:1100845/238‑1 (MQ=255)
                                                                                               gTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAAGTTGCGGGGAACAGCCAACCCCCCACCTTTTCCCCCTTTTTCCGTGCGGCAGGGGAGAACACACCTCAAAAAAAGCCCCCGGCACCAAAACATTTTGAGTTAACGTGCAGGGCAAAAACCCCTTCCcggt                                                                                                                                     >  1:424778/1‑237 (MQ=255)
                                                                                                  gACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAGGGTTCGGGAATCAGCAAACCCCCCCCTTTTTCCCGATTTTTCGGTGCGGCAGGGAAATACATCGCTAAAGAAAAGCCCACGGCCCCAAAGATTTTTTAGTTACCGTGCAGGGGAAAAACCCCCTCCGGGaaaaa                                                                                                                                 >  2:65011/1‑239 (MQ=255)
                                                                                                              gccAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTTAGGGTGGGGGAAGCAACCAGCCCCCCACCTTTTACCCATTTTTCGGGGCGGCGGGGAAGTACACCGCCAAAGAAATTACCCCGGGCCCAAAGAATTTTTTTGTATCGGGCCGGGCGAAAACGACCTTCCGGGACAAAGAttttcaagg                                                                                                                     >  1:15665/1‑233 (MQ=255)
                                                                                                              gccAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAAGGTTCGGGGATCAAGAAAGCCCCCCCTTTTTACCGATTTTTCGGTGCGGCGGGGAAGTAAATAGCTTAAAAAAATCCCCCGGCCCCCCAGAATTTTTAGTTAACCGGCCCGGGAAAAACGCCCTCCCGGGaaaaaggtctctacg                                                                                                                       >  1:573412/1‑228 (MQ=255)
                                                                                                              gccAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAAGGTGCGGGGAACAAGAAGGCCCCCACCTTTTCCCGACTTTTCTGGGTGGCAGTGGAGGACAACGACTAAAAAAAACCCCCCGCCCCCAAGAAGTTTTTGTTACCGCGTCGGGGACAAACGCCCTCCCGGAACAACAACTTCAGCg                                                                                                                      >  1:21287/1‑238 (MQ=255)
                                                                                                                        cgggtcccGAAAACAGAAACGGTGAAATGAACAGGAGGGTAAATCCCAGCGGTGCGGAAATCCCCACCAGCAGGGCGGTTAGGGTGGGGGGAATAAGCAGGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCt                                                                                                            <  2:1168160/232‑1 (MQ=255)
                                                                                                                        cGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAGGGTGGGGGAAACACGAAACCCCCCCCTTTTTCCCCGTTTTTCCGTGCGGCAGTGAAGTAACACGCTAAAAAAAAGGCCCCCGCCCCCAAAGTTTTTTAGTTACCGGGCAGGGGAAAAACGCCCTTCCGGGACAACGATTTTCGCGCGTCGGGGCt                                                                                                            >  1:640789/1‑238 (MQ=255)
                                                                                                                              cccaaaaCAGCAACGGTAAAAGGAACAGAAAGGAAAATTCCAGCGGTCCGTTAATCCCCACCAGCATGGCGGTTAGGGTGGCGGAAACCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTg                                                                                                     <  2:599170/235‑1 (MQ=255)
                                                                                                                              ccGCAAACACACAGGGTAAAATAAACAGAAAGGAAAAGTCGAGCGGTTCGGTAACTCCCCCCGGCCGGGCGGTAAGGGGTGGGGAAACCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTg                                                                                                     <  1:1168003/239‑1 (MQ=255)
                                                                                                                                   aacaagaaagggtaaaataaaaGGAAAGTAAAATCCCAGCGGTTCGTAAATCCCCCCAGGCATGGGGGTTAAGGTGGCGGAAACCAGCAACCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAAct                                                                                                 <  2:578228/218‑1 (MQ=255)
                                                                                                                                        cAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGGTTCGGGAATCAAAGAGCCCGGCACTTTTACCCGGTTTTCCGGTGCGGCAGGGGAGGACAACCCTCAAGAAATGCCCCCGGCCCCCAAGAATTTTGTGGTACCGTGCCGGGCCAAAACGCCCTCCCGGGACAAAGATTTTAGCGGGTCGGCGCGCACACtagaccacctg                                                                                            >  1:615608/1‑228 (MQ=255)
                                                                                                                                         aggggTAAAATAAAGAGAAAGGAAAATTCCAGCGGTCGGTTAATCCCCACCAGCATGGCGGTTAAGGTGGGGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAg                                                                                          <  2:853302/236‑1 (MQ=255)
                                                                                                                                             gTGAAATGAACAGAAAGGAAAATTCCAGCGTTTCGGTAATCCCCACCAGCAGGGCGGTTAAGGTTGCGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGAtg                                                                                       <  2:1118046/238‑1 (MQ=255)
                                                                                                                                               aaaaTGAACAGGAAGGAAAATTCCGGCGTGCCGGAAATCCCCACCAGCATGGTGTTTAGGGTGGCGGGAACCAACAACCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGAtgct                                                                                     <  1:804903/237‑1 (MQ=255)
                                                                                                                                                 aaagAACAGGAAGGTAAATCCCGGCGGTCCGATAATCCCCACCAACAGGGCTGTTAAGGTTGGGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTgcg                                                                                  <  2:15665/236‑1 (MQ=255)
                                                                                                                                                 aaTGAAGAGAAGGGTAAATCCCGGGGGTTGGGTAATCCCCCCCGGCATGCGGTGTAAGTGGGCGGAAACCAGAAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTgcg                                                                                  <  2:745465/239‑1 (MQ=255)
                                                                                                                                                 aaTGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCCCCAGCAGGGCGGTTAAGGTGGCGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTgcg                                                                                  <  2:471051/239‑1 (MQ=255)
                                                                                                                                                   taaaCAGGAAGGAAAATCCCAGCGGTTCGATAACCCCCACCAGCATGGCGTTTAAGGTGGGGGGAACCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc                                                                                <  2:1099592/237‑1 (MQ=255)
                                                                                                                                                   tGAACAGCAAGGTAAATACCAGCGGTTCGTTAATTCCCACCAGCATGGCGGTTTAGGTTACGGGAATCAACAAGCCCGCCACCTTTACCCGATTTTCCGGTGCGGCAGTGAAGTGCATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc                                                                                <  2:876063/239‑1 (MQ=255)
                                                                                                                                                   tGAACAGAAAGGAAAATCCCAGCGTGGCGTTAATCCCCCCCAGCTTGTGGTTTAGGGTGGCGGGAATCAGCAAGGCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc                                                                                <  2:929592/239‑1 (MQ=255)
                                                                                                                                                   agAACAGTAAGTTAAACCCCAGTGGTGTGATATCCCCCCCCAGCATGGCGTTTAGGGGTGCGGAAACCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc                                                                                <  1:725604/238‑1 (MQ=255)
                                                                                                                                                    tgaaaaggagggaaatgtcgggCGGTTGGGAAATTCCCACCAGCATGGCGGTTAAGGGGGCGGGAAACAGCAGGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc                                                                                <  2:655189/218‑1 (MQ=255)
                                                                                                                                                        aGGAAGTTAAATTCCGGCGGTTTGGTAATCCCCACCAGGATGGCGTTTGTGGTTGCGGAAATCATCATGACCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGTGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTa                                                                           <  2:144653/239‑1 (MQ=255)
                                                                                                                                                         gaaaGGTAAATCCCGGCGGTCCGTAAACCCCCCCCAGCGTGGGGTTTAGGGTGGCGGAAACCAAAAACCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCGCGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTa                                                                           <  1:16444/236‑1 (MQ=255)
                                                                                                                                                                     tccgggggtcggttAATCCCCCCCAGGTGGGGGGTTAAGGTGGGGGGAATCAGAACGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATgcc                                                               <  1:1083222/226‑1 (MQ=255)
                                                                                                                                                                     tCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGTTGCGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGGGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCGCGGCACCAAAGATTTTTTAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGGACAAAGATTTTAGCGGCTCGGGGCCCAAAATGAACTCTTGCAGATGCTGCGCCCAGGACATCTGAATgc                                                                >  2:16444/1‑238 (MQ=255)
                                                                                                                                                                         gCGGTTCGGTAATTCCCACCAGCATGGCGGGTAAGGGTGCGGGGATCAGCAAGCCCCCCCCCTTTACCCGGTTTTCCGGTGCGGCAGGGAAGTACATCGCTTAAAAAAATCCCCCGGCCCCAAAGATTTTTTAGTTTCCGTGGAAGGCAAAACCGCCTTCCGGGAACAAAGATTTCAGCGGATCGGGGCTCCAAACGGACTCTTTCCCAAGCTGCcccccacacacacggcaggccg                                                             >  1:951874/1‑219 (MQ=255)
                                                                                                                                                                                    atccccACCAGCATGGCGGTTAAGGTTGCGGGAACAAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGc                                                <  2:615608/236‑1 (MQ=255)
                                                                                                                                                                                           gcacagcaggtgggTTAAGGTGGCGGAACAAAGCAACCCCGCCACCTTTACCCGATTGTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGAc                                           <  1:803234/227‑1 (MQ=255)
                                                                                                                                                                                           ccaGCTGGGCGGTTAGGGTGGCGGGAACCAGCACGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCa                                         <  1:992772/239‑1 (MQ=255)
                                                                                                                                                                                            cgcgaaTGGCGTTAAGGGTCGCGGAAATAAGAACGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCa                                         <  2:327359/234‑1 (MQ=255)
                                                                                                                                                                                            ccacaagggCGGTAAGGGGTGCGGAAATCAGAAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCa                                         <  2:549684/232‑1 (MQ=255)
                                                                                                                                                                                                cATGGCGGTTAAGGTTGCGGGAATCAGCAAGCCCGCCCCCTTTTCCCGATTTTCCGGTGCGGCAGGGAAGGACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTTAGTTACCGTGCAGGGCAAAAACCCCCTCCGGGAAAAACGATTTCAGCGGCTCGGCGCGCCAACCGAAACCTTTACGATTGTGCGCCCAGTGCACCCGAAAgccccccttcaccccttgcgggcccaaag                                     >  1:68902/1‑216 (MQ=255)
                                                                                                                                                                                                     cGGTTAAGGTTGGGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATaaa                                 <  2:1216924/238‑1 (MQ=255)
                                                                                                                                                                                                        cgttatggtgggCGGTAATCAGCCACCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATaaa                                 <  1:1179920/227‑1 (MQ=255)
                                                                                                                                                                                                          gtaaggtgggCGGAAATAACCAACCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACt                               <  2:687554/229‑1 (MQ=255)
                                                                                                                                                                                                            tAAGGTTGGGGAATTCACAAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCATTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTc                            <  2:520416/239‑1 (MQ=255)
                                                                                                                                                                                                             agggTTGCGGAAATCAGCAGGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCAGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTcc                           <  2:743909/237‑1 (MQ=255)
                                                                                                                                                                                                             agggTTGCGGAAATCAGCAACCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTcc                           <  2:640789/237‑1 (MQ=255)
                                                                                                                                                                                                                gTTCCGGAAATCAGCAAGCCCGCCACCTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGt                         <  2:664855/238‑1 (MQ=255)
                                                                                                                                                                                                                    cGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGGACATATGAATGCCGCCTTCAACCGCTGCCGGACCAAAGATAACCTGTCCGTAgtcg                    >  1:735657/1‑236 (MQ=255)
                                                                                                                                                                                                                           tCAGCAAGACCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGTGCTCAAACTGAACTCTTGCAGATGATGCGCCCAGTACATTTGAAAGCCCCCTTCCACAGCTGCCGGACCAAAGCTACATTGGCCGTAGagtaagggg              >  1:775094/1‑231 (MQ=255)
                                                                                                                                                                                                                           aaccacaaaccccGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACAGCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAAg                 <  2:661418/228‑1 (MQ=255)
                                                                                                                                                                                                                            cAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCACTACATCTGAATGCCGCATTCAACAGCTGCCAGTCCAAAGATAAACTGGCCGTCGATGaatcggt             >  1:689575/1‑234 (MQ=255)
                                                                                                                                                                                                                                    gCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAAGTGGTGTaaa        >  2:1081743/1‑238 (MQ=255)
                                                                                                                                                                                                                                         gCCCCTTTTTCCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAAGTGGTGTAAACCGGt   <  1:897927/239‑1 (MQ=255)
                                                                                                                                                                                                                                          ccACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAAg                 >  2:1002676/1‑224 (MQ=255)
                                                                                                                                                                                                                                          ccACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAAg                 <  1:1002676/224‑1 (MQ=255)
                                                                                                                                                                                                                                          ccACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAAGTGGTGTAAACCGGtt  >  2:277966/1‑239 (MQ=255)
                                                                                                                                                                                                                                           cACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAAGTGGTGTAAACCGGtt  <  1:414044/238‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   
CGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGTTAAGGTTGCGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAAGTGGTGTAAACCGGTT  >  NC_000913/3862456‑3862919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: