BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,927,429	G→T	6.7%	L92L (CTG→CTT)	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,927,429	0	G	T	6.7%	463.3 / 18.6	139	L92L (CTG→CTT)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand): ref base G (61/68); new base T (7/2); total (68/71)
Fisher's exact test for biased strand distribution p-value = 9.43e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-02

Only 100 of 141 total aligned reads displayed.

CTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGG  >  NC_000913/3927192‑3927667

cTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTTATTCACTGGCGAAGTGGAAACGTCAGCCCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGt                                                                                                                                                                                                                                                   >  1:833056/1‑239 (MQ=255)

cTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGt                                                                                                                                                                                                                                                   >  2:1136560/1‑239 (MQ=255)

      gAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGc                                                                                                                                                                                                                                             <  2:167925/239‑1 (MQ=255)

      agtaactcctggctgtgtacgaagtggatctgatccggcaggaacggtgccaggccaaactcttcgctaaccgcagcttcggttgctttaattcccgcccagatcgcctctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTGTACACGc                                                                                                                                                                                                                                             >  1:634065‑M1/220‑239 (MQ=255)

         aactcctggctgtgtacgaagtggatctgatccggcaggaacggtgccaggccaaactcttcgctaaccgcagcttcggttgctttaattcccgcccagatcgcctctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTGTACACGc                                                                                                                                                                                                                                             >  1:1139294‑M1/217‑236 (MQ=255)

            tcgcTTTTCTCGTGAACCGGAAGAACGGCATGGGATGATCGAAGACCGGGCGCCGATTCTTAGCCGTGTGGGGGATGGTATGCAGGATATCTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑a                                                                                                                                                                                                                                      <  1:88314/236‑1 (MQ=255)

                    ctcGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCtt                                                                                                                                                                                                                            <  2:84820/239‑1 (MQ=255)

                      cGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTc                                                                                                                                                                                                                           >  1:86646/1‑238 (MQ=255)

                      cGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCg                                                                                                                                                                                                                          <  2:15278/239‑1 (MQ=255)

                        tCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGc                                                                                                                                                                                                                         >  1:87720/1‑238 (MQ=255)

                         cAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGcc                                                                                                                                                                                                                        <  1:1136560/238‑1 (MQ=255)

                         cAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGcc                                                                                                                                                                                                                        <  2:1015019/238‑1 (MQ=255)

                                    acggcgggggCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGa                                                                                                                                                                                                             <  1:304656/231‑1 (MQ=255)

                                     cGTCTGGGGCTGATCGAAGTCCATGCGCCGATGCTTAGCCTTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAGGCGGTTCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCg                                                                                                                                                                                                          <  1:817562/239‑1 (MQ=255)

                                       tCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGGCC‑CCGTGAAGGCCg                                                                                                                                                                                                          >  1:941431/1‑237 (MQ=255)

                                       tCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCg                                                                                                                                                                                                          >  1:625057/1‑237 (MQ=255)

                                                gATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGGCTTTCTCCGt                                                                                                                                                                                               >  1:397256/1‑239 (MQ=255)

                                                 aTCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGtt                                                                                                                                                                                              >  1:265910/1‑239 (MQ=255)

                                                      gccaggccaaactcttcgctaaccgcagcttcggttgctttaattcccgcccagatcgcctctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTG‑TACAG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACt                                                                                                                                                                                         >  2:1050856‑M1/172‑239 (MQ=255)

                                                      aGTCCAGGCTCCGTTTTTTAGCCGTGTGGGGGATGGCTCGCAGTATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCCAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACCCGCTCATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                          <  1:809433/238‑1 (MQ=255)

                                                      aGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                          >  2:836846/1‑238 (MQ=255)

                                                      aGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                          <  2:377750/238‑1 (MQ=255)

                                                      aGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                          <  2:1109524/238‑1 (MQ=255)

                                                      aGTCCAGGCGCCGAGTCTGAGCCGGGTGGGGGATGGCACGCAGGATAACTTGGCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACGGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCCGCGCTGAAgtcgtgttgccctaaggtct                                                                                                                                                                                           <  1:480869/238‑20 (MQ=255)

                                                          cAGGCGCCGATTCTTAGCCGTGTGGCGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGt                                                                                                                                                                                     <  1:271932/239‑1 (MQ=255)

                                                          cAGGCGCCGATGCTTAGCCGTGTGGGGTATGGCACGCAGGATAACGTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGt                                                                                                                                                                                     <  1:835952/239‑1 (MQ=255)

                                                             gcgcCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTa                                                                                                                                                                                  >  1:1201662/1‑239 (MQ=255)

                                                               aactcttcgctaaccgcagcttcggttgctttaattcccgcccagatcgcctctaccgtgctttccagagtcgagaattgacgctcaccgtcgcccattacgcgttcccaggcccactggtcaacatagaccgagtgcaacggagaaagacggtctctctcgGGTCGAAGGGCTTT‑CATGTGCGTG‑TA‑CGGCCCTTCGCCC‑CGATGAAGACCGTCTTGCTCCGTTGCACTCGGTCTATg                                                                                                                                                                                >  1:897147‑M1/163‑239 (MQ=255)

                                                                ccGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGt                                                                                                                                                                               >  1:1127999/1‑239 (MQ=255)

                                                                ccGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGGAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGCAGTGGAAACGTCAGACCTTAGGTCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AC‑AG‑CCCTTCGCCC‑CGCTGAAGACCGTCTTTCTCCGTTGCACTAGGCCTCTGt                                                                                                                                                                               >  1:889627/1‑239 (MQ=255)

                                                                 cGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCT‑TTCATGTGCGTGTAC‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGtt                                                                                                                                                                              <  2:357591/239‑1 (MQ=255)

                                                                  gATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                          <  1:94217/226‑1 (MQ=255)

                                                                  gATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                          >  2:94217/1‑226 (MQ=255)

                                                                    ttccctaaccgcagcttcggttgcttgaattcccgccgcgaccgcctctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactgggcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTG‑TACAG‑CCCTTCGCCC‑CGATGAGGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGAc                                                                                                                                                                           >  1:376550‑M1/158‑239 (MQ=255)

                                                                      cgctaaccgcagcttcggttgctttaattcccgcccagatcgcctctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTG‑TACAG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCa                                                                                                                                                                         >  2:396370‑M1/156‑239 (MQ=255)

                                                                      cTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGGAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCa                                                                                                                                                                         <  2:688990/239‑1 (MQ=255)

                                                                      cTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCa                                                                                                                                                                         <  2:86646/239‑1 (MQ=255)

                                                                            cGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGa                                                                                                                                                                   <  2:941431/239‑1 (MQ=255)

                                                                              tgtgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                          >  2:209742/1‑214 (MQ=255)

                                                                               gtgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGGAAAAGGGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTAAACGCACATG‑AC‑AC‑CCCTTCTCCC‑CGCGGAAGACCGTGTTTCTCCGTGGCACTCGGCCTATGTTGCCCTGCGGGACta                                                                                                                                                                >  1:400491/1‑238 (MQ=255)

                                                                                tgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGACGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTgg                                                                                                                                                               >  2:463911/1‑239 (MQ=255)

                                                                                  ggggATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTggg                                                                                                                                                              <  1:368755/238‑1 (MQ=255)

                                                                                   ttcggttgctttaattcccgcccagatcgcctctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTG‑TACAG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGaa                                                                                                                                                            >  2:881‑M1/143‑239 (MQ=255)

                                                                                    ggATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGaa                                                                                                                                                            <  2:1037982/238‑1 (MQ=255)

                                                                                      aTGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGGAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAAcgc                                                                                                                                                         >  2:435096/1‑239 (MQ=255)

                                                                                          cACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTa                                                                                                                                                      <  1:836846/238‑1 (MQ=255)

                                                                                                   tAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTCCACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACg                                                                                                                                            >  2:1223080/1‑239 (MQ=255)

                                                                                                         gTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACCGTGAGc                                                                                                                                      >  1:66109/1‑239 (MQ=255)

                                                                                                           cGGGCTGTGCCAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGTAGTGGTTCATTCACTGGCGCAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGCAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTTTCCGTTGCACTCGGTCTATGTTGGCCACTGGGACTGGGAACGCGTTATCGGCGCCGGTGAGCg                                                                                                                                     >  1:864781/1‑238 (MQ=255)

                                                                                                           cGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGACACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCg                                                                                                                                     >  2:49310/1‑238 (MQ=255)

                                                                                                                 gtgAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACCCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAAt                                                                                                                                >  1:54929/1‑237 (MQ=255)

                                                                                                                   ctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTG‑TACAG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTg                                                                                                                                                                             >  1:878830‑M1/111‑190 (MQ=255)

                                                                                                                     aaaaGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTGCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCGCGa                                                                                                                          <  1:281608/239‑1 (MQ=255)

                                                                                                                            ttgtaGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCCGTGGTACTGGGAACGCGTAATGGGCAGCGGTGAGCCTCAATTCTCGACCTTGaa                                                                                                                   >  1:138160/5‑239 (MQ=255)

                                                                                                                            gTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGACGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGCGGGTTCGCGTCAATTCGCGACTCTGaa                                                                                                                   >  1:763952/1‑239 (MQ=255)

                                                                                                                            gTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATtct                                                                                                                             <  2:485312/229‑1 (MQ=255)

                                                                                                                              gCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGaaaa                                                                                                                 >  2:1104071/1‑239 (MQ=255)

                                                                                                                                   tAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCa                                                                                                              <  2:1153624/237‑1 (MQ=255)

                                                                                                                                    aaacgggAAAGCTCTGCCTGATGTCCAGTTCGAAGTGGTTCATTGACTGCCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACGTCAGCGCTGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACgg                                                                                                           <  1:152057/233‑1 (MQ=255)

                                                                                                                                    aaaaGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACgg                                                                                                           <  2:746825/239‑1 (MQ=255)

                                                                                                                                      aaGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTa                                                                                                         <  2:599574/239‑1 (MQ=255)

                                                                                                                                            aaGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCg                                                                                                   >  2:657320/1‑239 (MQ=255)

                                                                                                                                            aaGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCg                                                                                                   >  2:800738/1‑239 (MQ=255)

                                                                                                                                                     cTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGc                                                                                           <  1:1104071/238‑1 (MQ=255)

                                                                                                                                                                ttCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGc                                                                               >  2:1075721/1‑239 (MQ=255)

                                                                                                                                                                 tCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTaaa                                                                                 <  1:1035495/236‑1 (MQ=255)

                                                                                                                                                                   gAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAAc                                                                            <  2:675118/239‑1 (MQ=255)

                                                                                                                                                                          ttcattcaCTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGaa                                                                        >  1:1062253/1‑236 (MQ=255)

                                                                                                                                                                                   tGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGt                                                                <  2:711271/235‑1 (MQ=255)

                                                                                                                                                                                   tGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGt                                                                >  1:711271/1‑235 (MQ=255)

                                                                                                                                                                                   tGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGc                                                            >  2:633031/1‑239 (MQ=255)

                                                                                                                                                                                    ggCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCg                                                           <  2:87720/239‑1 (MQ=255)

                                                                                                                                                                                       gAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGaa                                                         <  2:245590/238‑1 (MQ=255)

                                                                                                                                                                                        aaGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTACGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAaga                                                       >  2:926979/1‑239 (MQ=255)

                                                                                                                                                                                                     aGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACACGGTCTGTGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTGTCGACTCTGAAAAGCACGGTAGAGGCTATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGc                                           >  1:962059/1‑238 (MQ=255)

                                                                                                                                                                                                          acggagaaagacggtcttcatcgGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGt                                      <  1:983874‑M1/215‑1 (MQ=255)

                                                                                                                                                                                                            aGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGtt                                     >  2:876056/1‑237 (MQ=255)

                                                                                                                                                                                                            aGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGCCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCGGGCAcctgt                                     >  1:1173861/1‑234 (MQ=255)

                                                                                                                                                                                                                gaaCCCGACTTGAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTGCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGAGCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGGGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGc                                <  1:784309/237‑1 (MQ=255)

                                                                                                                                                                                                                cAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGc                                <  2:833056/238‑1 (MQ=255)

                                                                                                                                                                                                                cAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGc                                <  1:181175/238‑1 (MQ=255)

                                                                                                                                                                                                                 aaCACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCg                              >  2:1121896/1‑239 (MQ=255)

                                                                                                                                                                                                                    acGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCAACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGAt                           <  1:302987/239‑1 (MQ=255)

                                                                                                                                                                                                                      gACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCa                         <  2:697316/239‑1 (MQ=255)

                                                                                                                                                                                                                        tcttcatcgGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGa                       <  1:462654‑M1/230‑1 (MQ=255)

                                                                                                                                                                                                                        tcttcatcgGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGAAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGa                       <  2:186300‑M1/230‑1 (MQ=255)

                                                                                                                                                                                                                         ttCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCGCTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGGCTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGAt                      <  1:513293/239‑1 (MQ=255)

                                                                                                                                                                                                                           cAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATcc                    <  2:1121666/239‑1 (MQ=255)

                                                                                                                                                                                                                               gcgGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACt                 <  2:59310/238‑1 (MQ=255)

                                                                                                                                                                                                                                  GGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGt             <  1:1066400‑M1/239‑1 (MQ=255)

                                                                                                                                                                                                                                  GGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGt             <  2:347976‑M1/239‑1 (MQ=255)

                                                                                                                                                                                                                                     GAAGTGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCAC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTac           >  1:463911‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                                     GAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTac           >  2:1201662‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                                      AAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTaca          >  1:1112793‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                                           CTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGcc     >  1:800738‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                                            TGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCa    >  2:405231‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                                            TGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAg   <  2:211901‑M1/239‑1 (MQ=255)

                                                                                                                                                                                                                                            TGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGCTTACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGACAAGCACGGTCGAGGCGATCTGGGCGGGAATTACAGCAACCGAATCTGCGGTTAGCGAAGAGTTTAGCCTTGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAg   <  1:741512‑M1/239‑1 (MQ=255)

                                                                                                                                                                                                                                             gTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAgg  >  2:629654/1‑239 (MQ=255)

                                                                                                                                                                                                                                             gTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAACTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGAACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAgg  >  2:51509/1‑239 (MQ=255)

CTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATG‑AA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGG  >  NC_000913/3927192‑3927667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`