BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,927,436	C→T	7.1%	H95Y (CAC→TAC) ‡	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,927,436	0	C	T	7.1%	420.6 / 18.7	139	H95Y (CAC→TAC) ‡	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand): ref base C (61/67); new base T (7/3); total (69/70)
Fisher's exact test for biased strand distribution p-value = 2.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01

Only 100 of 142 total aligned reads displayed.

GAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTT  >  NC_000913/3927198‑3927671

gAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGc                                                                                                                                                                                                                                                <  2:167925/239‑1 (MQ=255)

agtaactcctggctgtgtacgaagtggatctgatccggcaggaacggtgccaggccaaactcttcgctaaccgcagcttcggttgctttaattcccgcccagatcgcctctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTGTACACGc                                                                                                                                                                                                                                                >  1:634065‑M1/220‑239 (MQ=255)

   aactcctggctgtgtacgaagtggatctgatccggcaggaacggtgccaggccaaactcttcgctaaccgcagcttcggttgctttaattcccgcccagatcgcctctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTGTACACGc                                                                                                                                                                                                                                                >  1:1139294‑M1/217‑236 (MQ=255)

      tcgcTTTTCTCGTGAACCGGAAGAACGGCATGGGATGATCGAAGACCGGGCGCCGATTCTTAGCCGTGTGGGGGATGGTATGCAGGATATCTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGa                                                                                                                                                                                                                                          <  1:88314/236‑1 (MQ=255)

              ctcGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCtt                                                                                                                                                                                                                                <  2:84820/239‑1 (MQ=255)

              ctcGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCtt                                                                                                                                                                                                                                <  2:65358/239‑1 (MQ=255)

                cGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTc                                                                                                                                                                                                                               >  1:86646/1‑238 (MQ=255)

                cGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCg                                                                                                                                                                                                                              <  2:15278/239‑1 (MQ=255)

                   cAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGcc                                                                                                                                                                                                                            <  1:1136560/238‑1 (MQ=255)

                   cAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGcc                                                                                                                                                                                                                            <  2:1015019/238‑1 (MQ=255)

                              acggcgggggCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGa                                                                                                                                                                                                                 <  1:304656/231‑1 (MQ=255)

                              aCGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGa                                                                                                                                                                                                                 <  1:572407/237‑1 (MQ=255)

                               cGTCTGGGGCTGATCGAAGTCCATGCGCCGATGCTTAGCCTTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAGGCGGTTCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCg                                                                                                                                                                                                              <  1:817562/239‑1 (MQ=255)

                               cGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCCGCGCTGAAgtcg                                                                                                                                                                                                               >  2:480869/1‑236 (MQ=255)

                                          gATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGGCTTTCTCCGt                                                                                                                                                                                                   >  1:397256/1‑239 (MQ=255)

                                           aTCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGtt                                                                                                                                                                                                  >  1:265910/1‑239 (MQ=255)

                                           aTCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGCGCGAAGGGCTGTAAACCCACATGAA‑AG‑CCCTTCCCCC‑CGATGAAGACCGTCTTTTTCCGt                                                                                                                                                                                                   >  1:944447/1‑238 (MQ=255)

                                               aaGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGCTGAAGACCGTCTTTCTCCGTTGCa                                                                                                                                                                                               >  1:823920/1‑238 (MQ=255)

                                                gccaggccaaactcttcgctaaccgcagcttcggttgctttaattcccgcccagatcgcctctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTGTACAG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACt                                                                                                                                                                                             >  2:1050856‑M1/172‑239 (MQ=255)

                                                aGTCCAGGCTCCGTTTTTTAGCCGTGTGGGGGATGGCTCGCAGTATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCCAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACCCGCTCATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                              <  1:809433/238‑1 (MQ=255)

                                                aGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                              <  2:377750/238‑1 (MQ=255)

                                                aGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                              >  2:836846/1‑238 (MQ=255)

                                                aGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                              <  2:1109524/238‑1 (MQ=255)

                                                aGTCCAGGCGCCGAGTCTGAGCCGGGTGGGGGATGGCACGCAGGATAACTTGGCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACGGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCCGCGCTGAAgtcgtgttgccctaaggtct                                                                                                                                                                                               <  1:480869/238‑20 (MQ=255)

                                                    ggccaaactcttcgctaaccgcagcttcggttgctttaattcccgcccagatcgcctctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTGTACAG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCgg                                                                                                                                                                                          <  2:376550‑M1/71‑1 (MQ=255)

                                                    cAGGCGCCGATTCTTAGCCGTGTGGCGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGt                                                                                                                                                                                         <  1:271932/239‑1 (MQ=255)

                                                       gcgcCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTa                                                                                                                                                                                      >  1:1201662/1‑239 (MQ=255)

                                                         aactcttcgctaaccgcagcttcggttgctttaattcccgcccagatcgcctctaccgtgctttccagagtcgagaattgacgctcaccgtcgcccattacgcgttcccaggcccactggtcaacatagaccgagtgcaacggagaaagacggtctctctcgGGTCGAAGGGCTTT‑CATGTGCGTGTA‑CGGCCCTTCGCCC‑CGATGAAGACCGTCTTGCTCCGTTGCACTCGGTCTATg                                                                                                                                                                                    >  1:897147‑M1/163‑239 (MQ=255)

                                                          ccGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGt                                                                                                                                                                                   >  1:1127999/1‑239 (MQ=255)

                                                            gATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                              >  2:94217/1‑226 (MQ=255)

                                                            gATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                              <  1:94217/226‑1 (MQ=255)

                                                                cTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGGAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCa                                                                                                                                                                             <  2:688990/239‑1 (MQ=255)

                                                                cTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCa                                                                                                                                                                             <  2:86646/239‑1 (MQ=255)

                                                                  tAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGt                                                                                                                                                                           <  1:329779/239‑1 (MQ=255)

                                                                      cGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGa                                                                                                                                                                       <  2:941431/239‑1 (MQ=255)

                                                                        tgtgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                              <  1:209742/214‑1 (MQ=255)

                                                                        tgtgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                              >  2:209742/1‑214 (MQ=255)

                                                                          tgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGACGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTgg                                                                                                                                                                   >  2:463911/1‑239 (MQ=255)

                                                                            ggggATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTggg                                                                                                                                                                  <  1:368755/238‑1 (MQ=255)

                                                                             ttcggttgctttaattcccgcccagatcgcctctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTGTACAG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGaa                                                                                                                                                                >  2:881‑M1/143‑239 (MQ=255)

                                                                                    cACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTa                                                                                                                                                          <  1:836846/238‑1 (MQ=255)

                                                                                       gtaatgcccgcccaaatcgcctctaccgatcgtttcagagtccagaaatgacgatcaccgaagcccatcacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTGTACAG‑CCCTGCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATgg                                                                                                                                                      >  1:861785‑M1/133‑239 (MQ=255)

                                                                                         aGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGc                                                                                                                                                    >  2:1112793/1‑239 (MQ=255)

                                                                                               aCTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGt                                                                                                                                              <  2:15760/239‑1 (MQ=255)

                                                                                                   gTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACCGTGAGc                                                                                                                                          >  1:66109/1‑239 (MQ=255)

                                                                                                     cGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCg                                                                                                                                         >  1:746825/1‑238 (MQ=255)

                                                                                                     cGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCg                                                                                                                                         >  2:1162133/1‑238 (MQ=255)

                                                                                                             ctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTGTACAG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTg                                                                                                                                                                                 >  1:878830‑M1/111‑190 (MQ=255)

                                                                                                               aaaaGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTGCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCGCGa                                                                                                                              <  1:281608/239‑1 (MQ=255)

                                                                                                                      ttgtaGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCCGTGGTACTGGGAACGCGTAATGGGCAGCGGTGAGCCTCAATTCTCGACCTTGaa                                                                                                                       >  1:138160/5‑239 (MQ=255)

                                                                                                                      gTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATtct                                                                                                                                 <  2:485312/229‑1 (MQ=255)

                                                                                                                      gTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATtct                                                                                                                                 >  1:485312/1‑229 (MQ=255)

                                                                                                                      gTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGCCTCTGaa                                                                                                                       >  2:742752/1‑239 (MQ=255)

                                                                                                                        gCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGaaaa                                                                                                                     >  2:1104071/1‑239 (MQ=255)

                                                                                                                          gtgTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAg                                                                                                                    <  2:1018317/236‑1 (MQ=255)

                                                                                                                              aaacgggAAAGCTCTGCCTGATGTCCAGTTCGAAGTGGTTCATTGACTGCCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACGTCAGCGCTGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACgg                                                                                                               <  1:152057/233‑1 (MQ=255)

                                                                                                                                aaGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTa                                                                                                             <  2:599574/239‑1 (MQ=255)

                                                                                                                                 aGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCAACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTag                                                                                                            >  2:302987/1‑239 (MQ=255)

                                                                                                                                      aaGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCg                                                                                                       >  2:800738/1‑239 (MQ=255)

                                                                                                                                               cTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGc                                                                                               <  1:1104071/238‑1 (MQ=255)

                                                                                                                                                           tCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTaaa                                                                                     <  1:1035495/236‑1 (MQ=255)

                                                                                                                                                             gAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAAc                                                                                <  2:675118/239‑1 (MQ=255)

                                                                                                                                                                    ttcattcaCTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGaa                                                                            >  1:1062253/1‑236 (MQ=255)

                                                                                                                                                                             tGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGACTTAAAGCAACCGAAGCTGCGGTTAga                                                                >  1:932924/1‑238 (MQ=255)

                                                                                                                                                                             tGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGt                                                                    <  2:711271/235‑1 (MQ=255)

                                                                                                                                                                             tGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGt                                                                    >  1:711271/1‑235 (MQ=255)

                                                                                                                                                                             tGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGc                                                                >  2:106545/1‑239 (MQ=255)

                                                                                                                                                                             tGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGc                                                                >  2:633031/1‑239 (MQ=255)

                                                                                                                                                                              ggCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCg                                                               <  2:87720/239‑1 (MQ=255)

                                                                                                                                                                                 gAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGaa                                                             <  2:245590/238‑1 (MQ=255)

                                                                                                                                                                                  aaGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTACGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAaga                                                           >  2:926979/1‑239 (MQ=255)

                                                                                                                                                                                      ggAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGTCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGtt                                                        <  1:435096/238‑1 (MQ=255)

                                                                                                                                                                                              cAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGc                                               <  2:423296/239‑1 (MQ=255)

                                                                                                                                                                                               aGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGc                                               >  2:201681/1‑238 (MQ=255)

                                                                                                                                                                                                    acggagaaagacggtcttcatcgGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGt                                          <  1:983874‑M1/215‑1 (MQ=255)

                                                                                                                                                                                                      aGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGtt                                         <  1:876056/237‑1 (MQ=255)

                                                                                                                                                                                                      aGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGtt                                         >  2:876056/1‑237 (MQ=255)

                                                                                                                                                                                                      aGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGCCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCGGGCAcctgt                                         >  1:1173861/1‑234 (MQ=255)

                                                                                                                                                                                                          gaaCCCGACTTGAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTGCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGAGCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGGGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGc                                    <  1:784309/237‑1 (MQ=255)

                                                                                                                                                                                                          cAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGc                                    <  2:833056/238‑1 (MQ=255)

                                                                                                                                                                                                           aaCACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCg                                  >  2:1121896/1‑239 (MQ=255)

                                                                                                                                                                                                              acGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGAt                               <  2:1127999/239‑1 (MQ=255)

                                                                                                                                                                                                                gACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCa                             <  2:697316/239‑1 (MQ=255)

                                                                                                                                                                                                                  tcttcatcgGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGa                           <  1:462654‑M1/230‑1 (MQ=255)

                                                                                                                                                                                                                   ttCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCGCTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGGCTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGAt                          <  1:513293/239‑1 (MQ=255)

                                                                                                                                                                                                                     ccagcgGGGCGAGGGGCGGTCCCCGAACATGAA‑CG‑CCCTTCGCCC‑CGATGAGGCCGGTCTTTCTCGGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGAAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCACCCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATcc                        >  1:396370‑M1/7‑239 (MQ=255)

                                                                                                                                                                                                                         gcgGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACt                     <  2:59310/238‑1 (MQ=255)

                                                                                                                                                                                                                            GGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGt                 <  1:1066400‑M1/239‑1 (MQ=255)

                                                                                                                                                                                                                            GGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGt                 <  2:347976‑M1/239‑1 (MQ=255)

                                                                                                                                                                                                                               GAAGGGCTGTACACGCACATGAA‑GT‑CCCTGCGCCC‑CGATGAAGACCTTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTac               >  1:881‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                               GAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTac               >  2:1201662‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                                AAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTaca              >  1:1112793‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                                     CTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGcc         >  1:800738‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                                      TGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCa        >  2:405231‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                                      TGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGCTTACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGACAAGCACGGTCGAGGCGATCTGGGCGGGAATTACAGCAACCGAATCTGCGGTTAGCGAAGAGTTTAGCCTTGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAg       <  1:741512‑M1/239‑1 (MQ=255)

                                                                                                                                                                                                                                       gTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAgg      >  2:629654/1‑239 (MQ=255)

                                                                                                                                                                                                                                       gTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAACTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGAACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAgg      >  2:51509/1‑239 (MQ=255)

                                                                                                                                                                                                                                        tACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGCGAGCGTCAATTCTCGACTCTGAACAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGACGCTGCGGTTAGCGGAGGGTGTGGCCTGGCACCGTTCCTGCCGGACAAGAGCAACGTCGTACACAGCCAGGa     >  1:282910/1‑239 (MQ=255)

                                                                                                                                                                                                                                             gCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGtt  >  1:575696/1‑237 (MQ=255)

                                                                                                                                                                                                                                             gCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGtt  >  2:507206/1‑237 (MQ=255)

GAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTT  >  NC_000913/3927198‑3927671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`