BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,927,442	A→T	5.6%	K97* (AAA→TAA)	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,927,442	0	A	T	5.6%	549.9 / 14.5	154	K97* (AAA→TAA)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand): ref base A (75/70); new base T (7/2); total (82/72)
Fisher's exact test for biased strand distribution p-value = 1.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

TCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACT  >  NC_000913/3927204‑3927674

tcgcTTTTCTCGTGAACCGGAAGAACGGCATGGGATGATCGAAGACCGGGCGCCGATTCTTAGCCGTGTGGGGGATGGTATGCAGGATATCTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGa                                                                                                                                                                                                                                             <  1:88314/236‑1 (MQ=255)

        ctcGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCtt                                                                                                                                                                                                                                   <  2:84820/239‑1 (MQ=255)

          cGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTc                                                                                                                                                                                                                                  >  1:86646/1‑238 (MQ=255)

          cGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCg                                                                                                                                                                                                                                 <  2:15278/239‑1 (MQ=255)

            tCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGc                                                                                                                                                                                                                                >  1:87720/1‑238 (MQ=255)

             cAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGcc                                                                                                                                                                                                                               <  1:1136560/238‑1 (MQ=255)

                         cGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCCGCGCTGAAgtcg                                                                                                                                                                                                                  >  2:480869/1‑236 (MQ=255)

                           tCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCg                                                                                                                                                                                                                 >  1:697316/1‑237 (MQ=255)

                                     aTCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGCGCGAAGGGCTGTAAACCCACATGAA‑AG‑CCCTTCCCCC‑CGATGAAGACCGTCTTTTTCCGt                                                                                                                                                                                                      >  1:944447/1‑238 (MQ=255)

                                        gAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCGTGATGCCCAGTGCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCa                                                                                                                                                                                                  <  2:695915/239‑1 (MQ=255)

                                         aaGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGCTGAAGACCGTCTTTCTCCGTTGCa                                                                                                                                                                                                  >  1:823920/1‑238 (MQ=255)

                                          aGTCCAGGCTCCGTTTTTTAGCCGTGTGGGGGATGGCTCGCAGTATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCCAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACCCGCTCATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                                 <  1:809433/238‑1 (MQ=255)

                                          aGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                                 <  2:1109524/238‑1 (MQ=255)

                                          aGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                                 <  2:931176/238‑1 (MQ=255)

                                          aGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                                 <  2:377750/238‑1 (MQ=255)

                                          aGTCCAGGCGCCGAGTCTGAGCCGGGTGGGGGATGGCACGCAGGATAACTTGGCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACGGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCCGCGCTGAAgtcgtgttgccctaaggtct                                                                                                                                                                                                  <  1:480869/238‑20 (MQ=255)

                                              ggccaaactcttcgctaaccgcagcttcggttgctttaattcccgcccagatcgcctctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTGTACAG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCgg                                                                                                                                                                                             <  2:376550‑M1/71‑1 (MQ=255)

                                              cAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGt                                                                                                                                                                                            <  1:1127232/239‑1 (MQ=255)

                                              cAGGCGCCGATTCTTAGCCGTGTGGCGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGt                                                                                                                                                                                            <  1:271932/239‑1 (MQ=255)

                                              cAGGCGCCGATGCTTAGCCGTGTGGGGTATGGCACGCAGGATAACGTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGt                                                                                                                                                                                            <  1:835952/239‑1 (MQ=255)

                                                 gcgcCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTa                                                                                                                                                                                         >  1:1201662/1‑239 (MQ=255)

                                                   aactcttcgctaaccgcagcttcggttgctttaattcccgcccagatcgcctctaccgtgctttccagagtcgagaattgacgctcaccgtcgcccattacgcgttcccaggcccactggtcaacatagaccgagtgcaacggagaaagacggtctctctcgGGTCGAAGGGCTTT‑CATGTGCGTGTA‑CGGCCCTTCGCCC‑CGATGAAGACCGTCTTGCTCCGTTGCACTCGGTCTATg                                                                                                                                                                                       >  1:897147‑M1/163‑239 (MQ=255)

                                                      gATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                                 <  1:94217/226‑1 (MQ=255)

                                                          cTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCa                                                                                                                                                                                <  2:86646/239‑1 (MQ=255)

                                                            tAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGt                                                                                                                                                                              <  1:329779/239‑1 (MQ=255)

                                                                cGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGa                                                                                                                                                                          <  2:941431/239‑1 (MQ=255)

                                                                  tgtgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                                 >  2:209742/1‑214 (MQ=255)

                                                                  tgtgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCAc                                                                                                                                                                                                 <  1:209742/214‑1 (MQ=255)

                                                                  tgtgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTggg                                                                                                                                                                           >  1:1153624/1‑236 (MQ=255)

                                                                   gtgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGGAAAAGGGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTAAACGCACATGAC‑AC‑CCCTTCTCCC‑CGCGGAAGACCGTGTTTCTCCGTGGCACTCGGCCTATGTTGCCCTGCGGGACta                                                                                                                                                                       >  1:400491/1‑238 (MQ=255)

                                                                    tgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGACGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTgg                                                                                                                                                                      >  2:463911/1‑239 (MQ=255)

                                                                      ggggATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTggg                                                                                                                                                                     <  1:368755/238‑1 (MQ=255)

                                                                       ttcggttgctttaattcccgcccagatcgcctctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTGTACAG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGaa                                                                                                                                                                   >  2:881‑M1/143‑239 (MQ=255)

                                                                        ggATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGaa                                                                                                                                                                   <  2:1037982/238‑1 (MQ=255)

                                                                          aTGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGGAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAAcgc                                                                                                                                                                >  2:435096/1‑239 (MQ=255)

                                                                                   aGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGc                                                                                                                                                       >  2:1112793/1‑239 (MQ=255)

                                                                                       tAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTCCACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACg                                                                                                                                                   >  2:1223080/1‑239 (MQ=255)

                                                                                             gTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACCGTGAGc                                                                                                                                             >  1:66109/1‑239 (MQ=255)

                                                                                               cGGGCTGTGCCAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGTAGTGGTTCATTCACTGGCGCAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGCAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTTTCCGTTGCACTCGGTCTATGTTGGCCACTGGGACTGGGAACGCGTTATCGGCGCCGGTGAGCg                                                                                                                                            >  1:864781/1‑238 (MQ=255)

                                                                                               cGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCg                                                                                                                                            >  2:1162133/1‑238 (MQ=255)

                                                                                               cGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCg                                                                                                                                            >  1:746825/1‑238 (MQ=255)

                                                                                                     gtgAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACCCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAAt                                                                                                                                       >  1:54929/1‑237 (MQ=255)

                                                                                                       ctaccgtgcttttcagagtcgagaattgacgctcaccgtcgcccattacgcgttcccagtcccactggtcaacatagaccgagtgcaacggagaaagacggtcttcatcgGGGCGAAGGGCTTT‑CATGTGCGTGTACAG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTg                                                                                                                                                                                    <  2:878830‑M1/80‑1 (MQ=255)

                                                                                                           aaGCGGTGCAGGTAAAAGAGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGAct                                                                                                                               <  2:834195/239‑1 (MQ=255)

                                                                                                                gTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGACGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGCGGGTTCGCGTCAATTCGCGACTCTGaa                                                                                                                          >  1:763952/1‑239 (MQ=255)

                                                                                                                gTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATtct                                                                                                                                    <  2:485312/229‑1 (MQ=255)

                                                                                                                gTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGCCTCTGaa                                                                                                                          >  2:742752/1‑239 (MQ=255)

                                                                                                                    gtgTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAg                                                                                                                       <  2:1018317/236‑1 (MQ=255)

                                                                                                                    aGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGTAACCCGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCG‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAg                                                                                                                       <  1:134377/238‑1 (MQ=255)

                                                                                                                        aaacgggAAAGCTCTGCCTGATGTCCAGTTCGAAGTGGTTCATTGACTGCCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACGTCAGCGCTGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACgg                                                                                                                  <  1:152057/233‑1 (MQ=255)

                                                                                                                          aaGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTa                                                                                                                <  2:599574/239‑1 (MQ=255)

                                                                                                                           aGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCAACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTag                                                                                                               >  2:302987/1‑239 (MQ=255)

                                                                                                                                aaGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCg                                                                                                          >  2:800738/1‑239 (MQ=255)

                                                                                                                                aaGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCg                                                                                                          >  2:657320/1‑239 (MQ=255)

                                                                                                                                                    ttCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGc                                                                                      >  2:1075721/1‑239 (MQ=255)

                                                                                                                                                     tCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTaaa                                                                                        <  1:1035495/236‑1 (MQ=255)

                                                                                                                                                       gAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAAc                                                                                   <  2:675118/239‑1 (MQ=255)

                                                                                                                                                                       tGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGACTTAAAGCAACCGAAGCTGCGGTTAga                                                                   >  1:932924/1‑238 (MQ=255)

                                                                                                                                                                       tGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGc                                                                   >  2:106545/1‑239 (MQ=255)

                                                                                                                                                                       tGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGc                                                                   >  2:633031/1‑239 (MQ=255)

                                                                                                                                                                                ggAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGTCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGtt                                                           <  1:435096/238‑1 (MQ=255)

                                                                                                                                                                                ggAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGtt                                                           <  1:1075721/238‑1 (MQ=255)

                                                                                                                                                                                              acggagaaagacggtcttcatcgGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGt                                             <  1:983874‑M1/215‑1 (MQ=255)

                                                                                                                                                                                                aGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGtt                                            >  2:876056/1‑237 (MQ=255)

                                                                                                                                                                                                    gaaCCCGACTTGAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTGCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGAGCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGGGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGc                                       <  1:784309/237‑1 (MQ=255)

                                                                                                                                                                                                     aaCACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCg                                     >  2:1121896/1‑239 (MQ=255)

                                                                                                                                                                                                       cacGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGa                                   >  1:275264/1‑239 (MQ=255)

                                                                                                                                                                                                        acGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGAt                                  <  2:1127999/239‑1 (MQ=255)

                                                                                                                                                                                                        acGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCAACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGAt                                  <  1:302987/239‑1 (MQ=255)

                                                                                                                                                                                                          gACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCa                                <  2:697316/239‑1 (MQ=255)

                                                                                                                                                                                                            tcttcatcgGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGAAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGa                              <  2:186300‑M1/230‑1 (MQ=255)

                                                                                                                                                                                                             ttCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCGCTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGGCTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGAt                             <  1:513293/239‑1 (MQ=255)

                                                                                                                                                                                                               ccagcgGGGCGAGGGGCGGTCCCCGAACATGAA‑CG‑CCCTTCGCCC‑CGATGAGGCCGGTCTTTCTCGGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGAAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCACCCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATcc                           >  1:396370‑M1/7‑239 (MQ=255)

                                                                                                                                                                                                                      GGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGt                    <  2:347976‑M1/239‑1 (MQ=255)

                                                                                                                                                                                                                      GGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGt                    <  1:1066400‑M1/239‑1 (MQ=255)

                                                                                                                                                                                                                         GAAGTGCTGTACACGCACATGAA‑AG‑CCCTTCGCAC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTac                  >  1:463911‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                         GAAGGGCTGTACACGCACATGAA‑GT‑CCCTGCGCCC‑CGATGAAGACCTTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTac                  >  1:881‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                          AAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTaca                 >  1:1112793‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                               CTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGcc            >  2:944447‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                               CTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGcc            >  1:800738‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                                TGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCa           >  2:405231‑M1/1‑238 (MQ=255)

                                                                                                                                                                                                                                TGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAg          <  2:211901‑M1/239‑1 (MQ=255)

                                                                                                                                                                                                                                TGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGCTTACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGACAAGCACGGTCGAGGCGATCTGGGCGGGAATTACAGCAACCGAATCTGCGGTTAGCGAAGAGTTTAGCCTTGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAg          <  1:741512‑M1/239‑1 (MQ=255)

                                                                                                                                                                                                                                 gTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAgg         >  2:629654/1‑239 (MQ=255)

                                                                                                                                                                                                                                 gTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAACTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGAACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAgg         >  2:51509/1‑239 (MQ=255)

                                                                                                                                                                                                                                  tACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGCGAGCGTCAATTCTCGACTCTGAACAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGACGCTGCGGTTAGCGGAGGGTGTGGCCTGGCACCGTTCCTGCCGGACAAGAGCAACGTCGTACACAGCCAGGa        >  1:282910/1‑239 (MQ=255)

                                                                                                                                                                                                                                       gCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGtt     >  2:507206/1‑237 (MQ=255)

                                                                                                                                                                                                                                       gCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGtt     >  1:575696/1‑237 (MQ=255)

                                                                                                                                                                                                                                         acaTGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGa                                                                 <  2:744126/175‑1 (MQ=255)

                                                                                                                                                                                                                                         acaTGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGtt     >  1:857210/1‑235 (MQ=255)

                                                                                                                                                                                                                                         acaTGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACt  <  2:54929/238‑1 (MQ=255)

                                                                                                                                                                                                                                            tGAG‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGCGGGACTGGGAACGCGTAATGGGCGCCGGTGTTCGTCCATTCTCTTCGCTGCAAAGCACGGTATAGCCGATCTGGGCGGGAATTAAATCCACCGAAGTTTCGGTTAGCGCAGGGTTAGGCCTGGCCCCGTTCCTCCCGGCTCAGCTCCACTTCTTACACATCCAgctgtt     >  1:260660/1‑227 (MQ=255)

                                                                                                                                                                                                                                            tGAA‑AG‑CCCTTCGCCC‑CGATGAATACCGTCTTTCACCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATTTGGGCTGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGCGTTTGGCCAGGCACCGTTCATGCCGGATCAGATCTACTCCGTACAAAGCCAGGAGtt     >  1:876933/1‑232 (MQ=255)

                                                                                                                                                                                                                                            tGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAACAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTac                  >  1:588988/1‑219 (MQ=255)

                                                                                                                                                                                                                                            tGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTACAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCCGATCCACTTCGTACACAGCCAGGCGtt     >  1:1055344/1‑232 (MQ=255)

                                                                                                                                                                                                                                            tGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGtt     >  1:816582/1‑232 (MQ=255)

                                                                                                                                                                                                                                            tGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGtt     >  2:840092/1‑232 (MQ=255)

                                                                                                                                                                                                                                            tGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGtt     >  2:189950/1‑232 (MQ=255)

                                                                                                                                                                                                                                            tGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGGAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGATCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGtt     >  2:747484/1‑232 (MQ=255)

                                                                                                                                                                                                                                            ggAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTac                  <  2:588988/218‑1 (MQ=255)

TCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAA‑AG‑CCCTTCGCCC‑CGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACT  >  NC_000913/3927204‑3927674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`