BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,256,068	G→C	18.4%	P133R (CCG→CGG)	plsB ←	glycerol‑3‑phosphate O‑acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,256,068	0	G	C	18.4%	251.8 / 14.2	97	P133R (CCG→CGG)	plsB	glycerol‑3‑phosphate O‑acyltransferase
Reads supporting (aligned to +/- strand): ref base G (11/67); new base C (17/2); total (28/69)
Fisher's exact test for biased strand distribution p-value = 7.35e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

GCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCG‑CCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAAC  >  NC_000913/4255831‑4256301

gCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCgg                                                                                                                                                                                                                                               >  2:422701/1‑239 (MQ=255)

 cAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCCCCTTTTTCACCCCCCgg                                                                                                                                                                                                                                               >  2:700914/1‑238 (MQ=255)

    gCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGAGTCACATCACCTTTTTTACGCCCCgtcac                                                                                                                                                                                                                                            >  2:65152/1‑234 (MQ=255)

     cAAACGTGGGCCTACGGCAGCCAGACGTTGCCGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTAGCCGTGCCGTGTTCATCCGCCATCCGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGgc‑gc‑g                                                                                                                                                                                                                                         <  2:1232452/238‑1 (MQ=255)

     cAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGgc‑g                                                                                                                                                                                                                                            <  2:751623/236‑1 (MQ=255)

        aCGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGGGCCGGGTTCAGCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGAc                                                                                                                                                                                                                                     <  1:59001/239‑1 (MQ=255)

           ggggCCTGGGGCGGCCAGACGTTGACGGGCAAAGTGCTGACGCGCCACGCGCCCCATTTTCTGAGCGATAGTTTTATCCGGCCCGTGTCCAACCGCCATCCGGCGCAGCGAAACTGACGGCGAGAACCG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCa                                                                                                                                                                                                                                   <  1:44037/237‑1 (MQ=255)

                   cGGCAGCTAGCCGGTGGGGGGCAAAGGCCACCCGCCCCCCGCGCGCCATTTTCGGAGCGATAGTTTTATCCGTGCCGTGTTCACCCGCCATACGGCGCAGCAAAACTGACGGCGAGAACCG‑CACAAAACTGCCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTCCGCCGTTAAGCATACGCAGCGGCGGGTTCCCTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCAcc                                                                                                                                                                                                                         <  1:908936/239‑1 (MQ=255)

                   cGGCAGCCGGACGTTGACGGGCAAAGTGCATACGCGCCCCGCGCGCCAGTTGCTGAGCGATAGTTTAATCCGTGCCGTGTTCATCCGCCAGACGGCGCAGCGAAACTGACGGCGAAAAACG‑CAAAAAACTGTCGCGACC‑GTGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCAcc                                                                                                                                                                                                                         <  1:1203310/239‑1 (MQ=255)

                   cGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTTACGCCCCGGC‑GC‑GCGGCCCAA‑AAacac                                                                                                                                                                                                                          >  2:667552/1‑238 (MQ=255)

                   cGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTTACCCCCCCTC‑GC‑GCCACCAAA‑CAacac                                                                                                                                                                                                                          >  2:908936/1‑238 (MQ=255)

                   cGGCAGCCAGACGTTGACGGGCAAAGCGCATACGCGCCACGCGCCCAAGTTTCTGAGCGATAGTTTATTCCGTGCCGTGTTCACCCGCCATGCGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGCCGCGGCC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCAcc                                                                                                                                                                                                                         <  1:551703/239‑1 (MQ=255)

                   cGGCACCCAGACGTGAGCGGGAAAAGTGCAGACGCGCCAGGCGCGCCAGTTTCGGAGCGTTGGTTTTACCCGTGCGGTGTCAACCCGCCATACGGCGCAGCGAAACTGACGGGGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCAcc                                                                                                                                                                                                                         <  1:983904/239‑1 (MQ=255)

                    ggaagccagccGTTGACGGGAAAGGTCCATCCGCGCCCCGCGCGCCAGTTTCTGAGCGATAGTTTTTCCCGTGCCGTTTTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCAcc                                                                                                                                                                                                                         <  1:760334/235‑1 (MQ=255)

                        gCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCCTTTTCACCCCCCCGC‑gc‑ccgccccacaaacacccacc                                                                                                                                                                                                                      >  1:540220/1‑224 (MQ=255)

                        gCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACCTTGTCCTTTTTGCACCCCGGC‑GC‑GCGACCCAA‑CATCACCAacc                                                                                                                                                                                                                     >  1:1059216/1‑237 (MQ=255)

                          cAGACGTTGACGGGCAAAGTGCATCCGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTACCCGTGCCGTTTTCATCCGCCATCCGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGaca                                                                                                                                                                                                                     <  2:335555/236‑1 (MQ=255)

                             agtttGACGGGCAAAGGGCATCCGCGCCACGCGCGCAAGTTTCTGGGCAATAGTTTTACCCGTCCCGTGTTCATCCCCCATACGGCGCAGCGAAACTGACGGCGAAAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGc                                                                                                                                                                                                                <  1:654364/235‑1 (MQ=255)

                              cGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGc                                                                                                                                                                                                                <  2:1003223/237‑1 (MQ=255)

                              agTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGGGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGc                                                                                                                                                                                                                <  2:176698/236‑1 (MQ=255)

                               gTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCAcc                                                                                                                                                                                                             <  2:241328/239‑1 (MQ=255)

                                 tgcggggCAAAGGGCATCCGCGCCACCCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCa                                                                                                                                                                                                            <  1:700914/234‑1 (MQ=255)

                                 tGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTCAAACCCCCGG‑CC‑GCGACCacaaataccccaccacggcaccc                                                                                                                                                                                                             >  2:851/1‑216 (MQ=255)

                                     gggCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGc                                                                                                                                                                                                       <  2:962799/239‑1 (MQ=255)

                                         aaaGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACAt                                                                                                                                                                                                   <  2:583776/239‑1 (MQ=255)

                                                aTACGCCCCACGCGCGCCAGTTTCGGAGCGATATTTTTATCCGTGCCGTGTTCATCCGCCATACGGGGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGAtt                                                                                                                                                                                            <  2:799635/239‑1 (MQ=255)

                                                 tACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCCCCCTTTTTACGCCCCCGC‑CC‑GCGACCCAA‑CCACACCGACACCGGCCCCAACCGCACATCCacggtt                                                                                                                                                                                           >  1:903549/1‑234 (MQ=255)

                                                     cgcCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTTACCCCCCGGC‑GC‑GCGAACAAA‑CATCACCGGCACTGGCACCATCTCCACATCCAGATTTTGGt                                                                                                                                                                                       >  2:827868/1‑239 (MQ=255)

                                                       ccACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTTACGCCCCCGC‑GC‑GCGACCCAA‑CACCACCCACACTGGCCCCATCTTCCCATCCAGATTTGGGtt                                                                                                                                                                                      >  2:755723/1‑238 (MQ=255)

                                                       ccACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTTACCCCCCGGC‑GC‑GCGGCCAAA‑CATCACCGCAACTGGCACCATCTGCCCATCCAGATTTGGGtt                                                                                                                                                                                      >  2:219097/1‑238 (MQ=255)

                                                       ccACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACCTCGCCTTTTTCACGCCCCGGC‑GC‑GGGGCCAAA‑CATCACCGACACTGGCACCATCCGCACCTCCAGACTTTGGttt                                                                                                                                                                                     >  2:976014/1‑238 (MQ=255)

                                                         aCGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCt                                                                                                                                                                                   <  2:677434/239‑1 (MQ=255)

                                                         aCGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCt                                                                                                                                                                                   <  2:1768/239‑1 (MQ=255)

                                                          ggcgcgcagtttcctggtcGATAATTTTATCCGGGCCGTCTCTACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAGCG‑CCCAAAACTGTCGCGACC‑GCGCCACAGTACAGCAAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTa                                                                                                                                                                                  <  1:685120/221‑1 (MQ=255)

                                                           gcgcgcCAGTTGCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTAc                                                                                                                                                                                 <  2:413760/239‑1 (MQ=255)

                                                           gcgcgcCAGTGTCGGAGCTATAGTTTTACCCGGGCCGTGTTCACCCGCCATCCGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTAc                                                                                                                                                                                 <  1:787455/239‑1 (MQ=255)

                                                               gcCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTg                                                                                                                                                                             <  2:279706/239‑1 (MQ=255)

                                                               gcCAGTTTCTGAGCGATAGTTTTACCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTg                                                                                                                                                                             <  2:228243/239‑1 (MQ=255)

                                                               gcCAGTTTCTGAGCGATAGTTTTACCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTg                                                                                                                                                                             <  2:561039/239‑1 (MQ=255)

                                                                       ttGAGCGAGAGTTTTCTCCGTGCCGTGTGCACCCCCCATACGGCTCAGCGAACCTGACGGCGAAAAACT‑CGCAAAACTGTCGCCACC‑GAGCCACAGTACAGCGAAAAATTTCTGTTCGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑CC‑GCGACCAAA‑CATCGCCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑ga                                                                                                                                                                    <  1:1094652/238‑1 (MQ=255)

                                                                          gggtaTAGTTTTATCCGTGCAGTGTACATCCGCCATACGCCGCAGCAAACCTGGCGGCGA‑AAACGTCCCAACGCTTTCGTGCCC‑GAGCCACAGTACAGCGAAAAATTTCTGTGCGCCGTTAAGCATGCGCCCCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACAATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑gaga                                                                                                                                                                  <  1:1009510/234‑1 (MQ=255)

                                                                           gagATAGTTTCATCCGTGCCCTGTCCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGAt                                                                                                                                                                 <  1:813342/236‑1 (MQ=255)

                                                                           gCGATAGTTTTATCCGGGCGCTGTCCATCCGCCATACGGGGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACGGTAGGGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGAt                                                                                                                                                                 <  1:563057/238‑1 (MQ=255)

                                                                           gCGATAGTTTTAGCCGTGCCGTGTCCACCCGCCATCCGGCGCAGCAAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGAt                                                                                                                                                                 <  1:422701/238‑1 (MQ=255)

                                                                           gCGAGAGTTTTATCCGTGCCGTGTTCACCCGCCATCCGGCGCAGCAAAACTGACGGCGAGAAACG‑CACAAACCTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGAt                                                                                                                                                                 <  1:697327/238‑1 (MQ=255)

                                                                             gATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTCACGCCCCCGC‑GC‑GCCACCCAA‑CATCACCCACCCTGGCACCCTCTGCACATCCAGATTTGGGGTGCTACGGGGCAAATC‑GAGATAGt                                                                                                                                                              >  2:697327/1‑239 (MQ=255)

                                                                              aTAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCCTTTTCTCCCCCCCGC‑GC‑CCGACCAAA‑CACCACCCCCCCTCGCCCCCTCTTCACCCCCCCATTTGGGGTGCTACGGGGCAAATC‑GAGCAAGTc                                                                                                                                                             >  1:750871/1‑239 (MQ=255)

                                                                              aTAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACATTGCCTTTTTCACGCCCCCGC‑GC‑GCGGCCCAA‑CATCACCGCCACCAGCCCCACCCGCACCACCCCACTTTGGGTGCTCCGGTGCAAAAC‑CAGATaca                                                                                                                                                              >  1:512239/1‑236 (MQ=255)

                                                                               tAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTTACGCCCCGGC‑CC‑GCGACCAAA‑CATCACCCACACTGGCACCCTCTGCCCATCCAGCTTTGGGTTGCTACGGGGCAAATC‑GAGATAGTCg                                                                                                                                                            >  2:781366/1‑239 (MQ=255)

                                                                                 gTTTTATCCGGGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTa                                                                                                                                                                                  <  2:533472/216‑1 (MQ=255)

                                                                                    ttacctggcccgtgTTCACCCGCCATACGGCGCAGCGAAACTGAGGGCGAGAATGG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCCCTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGaa                                                                                                                                                       <  1:633945/230‑1 (MQ=255)

                                                                                    ttATCCGTGCCGTTTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGaa                                                                                                                                                       <  2:9469/239‑1 (MQ=255)

                                                                                    ttATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAGCTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGaa                                                                                                                                                       <  2:415247/239‑1 (MQ=255)

                                                                                    ttATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGaa                                                                                                                                                       <  2:891797/239‑1 (MQ=255)

                                                                                    ttATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGaa                                                                                                                                                       <  2:540220/239‑1 (MQ=255)

                                                                                    ttATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGaa                                                                                                                                                       <  2:280249/239‑1 (MQ=255)

                                                                                    ttATCCGTGCCGTGTTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGaa                                                                                                                                                       <  1:956830/239‑1 (MQ=255)

                                                                                    ttACCCGGGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGaa                                                                                                                                                       <  2:750871/239‑1 (MQ=255)

                                                                                    ttACCCGGGCCGTGTCCATCCGCCAGACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGaa                                                                                                                                                       <  1:846836/239‑1 (MQ=255)

                                                                                    tacccgtcgctggttacctCCGCCATGCGGCGCAGCGAAACGGACGGGAAGAAACG‑CACAAAACGGTCGGGACC‑GAGCCTCAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGaa                                                                                                                                                       <  1:1041867/221‑1 (MQ=255)

                                                                                           tgccgtgTTCGTCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTTGCCTTTTTCACGCCCCCGC‑GC‑GCGGCCAAA‑CAACACCCACCCCCGCCCCCTCTGCCCACACAAGATTGGGGTGCTTCGGGGGAAATC‑GAGAAAgatgggggaaagccta                                                                                                                                                >  1:750426/1‑224 (MQ=255)

                                                                                               gtgtTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGAACAGCTTAATAg                                                                                                                                            <  1:715746/239‑1 (MQ=255)

                                                                                                 gtTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGAACAGCTTAATAGAc                                                                                                                                          <  2:1098786/239‑1 (MQ=255)

                                                                                                 gtTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTTACGCCCCGGC‑GC‑GCGCACCAA‑CATCACCGACACCTGCACCCCCCGCCCACCCCGACTTTGGTTGCTTCGGTGGCAATC‑GAGATAGTCAGGGAACCGCCTAATAGa                                                                                                                                           >  1:986219/1‑238 (MQ=255)

                                                                                                  tccaccccccATAGGGCGCAGCAAAACGGACGGCGAGAAACG‑CACAAAACGGTCGCGACC‑GCGCCACAGTACAGCGAAAAATTTCTGTACGCCGTAAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGAACAGCTTAATAGAc                                                                                                                                          <  1:816342/230‑1 (MQ=255)

                                                                                                  ctCATCCGCCATCCGGCCCATCGAACCTGACGGCGAGAAACG‑CCCAAACTTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGAACAGCTTAATAGAc                                                                                                                                          <  2:59943/237‑1 (MQ=255)

                                                                                                         gcaaTACGGCGCAGCGAAACTGACGGAGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGAACAGCTTAATAGACTCTTCttt                                                                                                                                  <  1:358864/236‑1 (MQ=255)

                                                                                                          ccATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTTACGCCCCCGC‑GC‑GCGGCCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGGTGCTACGGGGCAAATC‑GAGATAGTCGGGGGACAGCTTAATAGAATCTTCttt                                                                                                                                  >  2:32724/1‑238 (MQ=255)

                                                                                                          ccATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCCGC‑GC‑GCGGCCAAA‑AATCCCCGACACTGGCACCATCTTCACATCCAGATTTTGGTTGCTACGGTGCAAAAC‑GGGATAGTCGTGGAACAGCTTAATAGACTcctctt                                                                                                                                   >  2:338381/1‑237 (MQ=255)

                                                                                                           caaaCGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGACGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGGGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCg                                                                                                                                <  2:686521/236‑1 (MQ=255)

                                                                                                            acgcGGCTCAGCGAAACTGACGGAGATAAACG‑CACAAAACTTCCCGGCCC‑TAGCCACAGTACAGCGAAAAATTTTTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCgg                                                                                                                               <  1:81843/236‑1 (MQ=255)

                                                                                                             tACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGc                                                                                                                              <  2:986219/239‑1 (MQ=255)

                                                                                                             tACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGc                                                                                                                              <  2:47183/239‑1 (MQ=255)

                                                                                                                   acaagcaaccgagcgggagtacaCG‑CATAAGGCTGTGCCGACC‑GCGCCACAGTACAGCGAAAAATCGCTGTACGCCGTTAAGCATCCGCAGCCGCCGGTTCCCTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCACA‑CACCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTaa                                                                                                                        <  1:393916/217‑1 (MQ=255)

                                                                                                                    cAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTTACGCCCCGGC‑GC‑GCGGCCAAA‑CATCACCCACACCGGCCCCATCTGCACATCCCGATTTGGGGTGCTACGGTGCAAAAC‑GAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTTGGCGTGTAAt                                                                                                                       >  2:97989/1‑239 (MQ=255)

                                                                                                                       cGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTCACCGCCCCGC‑GC‑GCGGCCCAA‑CAACACCGACACCGGCACCCTCCGCACAACCAGCTTTGGGTTTCGACGGTGCAAATC‑CAGGAAATCGTGGGAACGCTTAATAGAATCTTCTTTCGGCGTGTAATaag                                                                                                                    >  1:423569/1‑237 (MQ=255)

                                                                                                                        gAAACGGACGGCGGGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTCCTGTACCCCTTTAAGCATACGCAGCGGCGGGTTCCCTTCGCCTTTTCCGCGCCCCGTC‑GC‑GCGACCAAA‑CAGCCCCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAgt                                                                                                                    <  2:405958/238‑2 (MQ=255)

                                                                                                                            cTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTTACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCCTCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GGGATAGTCGTGGGAAAGCTTAATAGAATCTTCTTTCGGGGTGTAATAgggtgac                                                                                                               >  2:1128726/1‑234 (MQ=255)

                                                                                                                                   caaaaaaCG‑CCAAAAACGGCCGCGACC‑GGGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTCCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACAcgc                                                                                                         <  2:1203226/234‑1 (MQ=255)

                                                                                                                                                 aaaacgTCGCGCCCGGAGCCACAGTACAGAGAAAAATTTTTGTACGCGTTAAAGCAGCGGCCGGGGGGGTTTCCCTCCGCTTTTTTC‑CCCCCCGGCGGG‑GCGACAAAA‑CACCCCCGACCCTGGCACAATCTGCACATCCAGATTGGGGTTGCTAGGGTGCAAATC‑GAGATAGTCGTGGAACAGTTTAATAGACTCTTCTTCCGGCGTGTAATAGGTGAACACACGCGGCCCG‑CCGtgaa                                                                                           <  1:97989/234‑1 (MQ=255)

                                                                                                                                                         cgccccggggccaagtcaCAGCAAAAAATTTCTGGCCGCCTTAAAGAATCCGCGGCGGGGGGTCCACTCCGCCTTTTCCACCCCCGGGC‑GG‑GCACCCAAC‑CACCCCCGACACTGGCACCATCTGAACATCCAGATTTGGGTTGCTGCGGTGAAAATG‑GAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGGGAACACACGCGGCCCG‑CCGTGAATGAAcaca                                                                                   <  1:723495/222‑1 (MQ=255)

                                                                                                                                                           gACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTAAAGCATACGCAGCGGCGGTTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CACCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCG‑CCGTGAATGAACACATAg                                                                                <  2:512239/239‑1 (MQ=255)

                                                                                                                                                            aCC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTTACCCCCCCGC‑CC‑GCGGACCAA‑CAACCCCCCCACCGGACCCACCTCCACACCCAGAATTGGGGTGGTACGGGGCAAACT‑GAGGTAGGCGTGGAACAGATTAAAAGACTCTTTTTTCGGCGCGTTATAAGGGAACAAACGCGGCCCC‑CCGTGAACGAAaacaaaa                                                                                >  1:828356/1‑235 (MQ=255)

                                                                                                                                                            aCC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCCGC‑GC‑GCGACCAAA‑AATCACCGACACTGGCACCCTCTGCACATCCCGATTTTGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGGAATAGGGGAACACACCCCGCCCC‑CCCCGAATGAACACATAg                                                                                >  2:666076/1‑238 (MQ=255)

                                                                                                                                                            aCC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACCTCGCCCCTTTTACCCCCCCGC‑GC‑CCGACCAAA‑AATCACCGACACCCGTCCCATCTGCCCATCCACATTTGGGGTGCTCCCGGGCCAAAC‑GGGATAGTCGTGGACAACGTTAATAAAATCTTTTTTCGGTGGGGGAAAGGGGTACACACGCCGCCCGTCCATG‑ATGAAcacaacat                                                                               >  1:973920/1‑235 (MQ=255)

                                                                                                                                                              ccgcccaccggcaccaacaaaatTTTTTGTCCGCCGTTAAGCTACCGCCCCGGGGGGTTCTCTCCCCCTTTTCCCCGCCCCGGC‑CG‑GCGCCAAAA‑CACCCCCGACACGGCCCCCATCTGCACATCCAGTTTTGGGTTGCTACGGGGCAAATC‑GAGATAGTCGGGGAACAGCTTAATAGCCTCTTCTTTCGGCGTGTAATAGGTGAACCCCCGCGGCCCG‑CCGTGAATGAACACATAgcg                                                                              <  1:1102997/221‑1 (MQ=255)

                                                                                                                                                                                   aaTTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCCAA‑CAACACCGCCACTGGCACCATCCGCCCATCCAGAGTTGGGTTGCTACGGGGCAAAAA‑GAGAAAGTCGTGGAACAGCTTAATAAACTCTTCTTTCTGCGGGTAAAAAGGGAACAAACACCGCCCC‑CCGTGAATGAGAACATAACGCGGGAggaaactgccctcgcg                                                         >  1:1081727/1‑224 (MQ=255)

                                                                                                                                                                                             accccataaaacacagccgccggggggTCACTTAGCGCTTGTCCCGCCCGCGG‑GCGGCGACAAAG‑CACCACCGGCACGGGCACCACCTGAACATCAAAATTTGGGTTGCACCGGTGCAAATG‑GAAATAGGCGGGGAGCAGCTTAACAGGCTCTGCTTGCGGCGTGAAGTGGGGGAACACCCGCGGCCCG‑CCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGGCGATTTCCAGCg                                                 <  1:159032/215‑1 (MQ=255)

                                                                                                                                                                                              gCCGTAAAGCATACGCAGCGGGGGTTTCCCTTCGCCTTTTCCACGCCCCGGC‑GC‑GCGACCAAA‑CACCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGACCCCACGCGGCCCG‑CCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGct                                              <  2:818644/239‑1 (MQ=255)

                                                                                                                                                                                                       cATCCGCGGCGGCGGTTTCACTGCCCTTTTTCCACCCCCGGGC‑GC‑GCGCCCAAA‑CACCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTCCTACGGTGCAAATC‑GAGATAGTCGTGGAACAGCTTAATAGCCTCTTCTTTCGGCGTGTAATGGGTGACCACACGCGGCCCG‑CCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGt                                     <  1:219097/239‑1 (MQ=255)

                                                                                                                                                                                                       atcccgcgcgggggggTCCATTCCGCCTTTTCCCCCCCCCGGC‑GC‑GGAACAAAA‑CATCCCCGACACTGGCACCATCTCCACATCCATATTTGGTTTGCTACGGTGCAAATC‑GAGATAGTCGTGGAACAGCTTAATAGCCTCTTCTTTCGGCGTTTAATAGGTGAACACACGCGGCCCG‑CCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGt                                     <  1:521641/226‑1 (MQ=255)

                                                                                                                                                                                                        atccGCAGCGGCGGGTTCCCTTCGCTTTTTTCACGCCCGGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATTGTCGTGGAACAGTTTAATAGACTCTTCTTTCGGCGTGTAATGGGTGAACACACGCGGCCCG‑CCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGt                                     <  2:743047/235‑1 (MQ=255)

                                                                                                                                                                                                          aCGCAGCGGCGGGTTCACTTTCCCCTTTTTACCCCCCCGC‑GC‑GCGACCAAA‑CAACACCCCCACCGGCACCCTCTTCACCTCCACATTTTGGGTGGTACGGGGCAAAAC‑GAGATAGTCGTGGGACCGCTTTATAGACCCTTCTTTCTGCGTGTAATAGGTGAACACACGCGGCCCG‑CCGCGGATGGACAACAAGCCCGGCCGTGACGGGGCGCGGATTTTCAGCGGGCATAACggggccg                                  >  1:41612/1‑235 (MQ=255)

                                                                                                                                                                                                                  gggggTCCCCTTCGCCTTTTCCCCCCCCCGGC‑GC‑GCGCCAAAA‑CACCCCCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCG‑CCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTc                           <  2:83241/236‑1 (MQ=255)

                                                                                                                                                                                                                      gTTTACTTTGCCTTTTTTTCGCCCCCGC‑GC‑GCGACCCAA‑CATCACCGACACCGGCACCATCTGCCCACCCAGATTTTGGGTGCTACGGGGCAAATC‑GAGAGAGTCGTGGGACAGCTTAATAGACTCTTCTTTCGGCGTGTAAAAAGTGAACACACCCGGCCCC‑CCCTGAATGAACACATAGCGCGGCAGGAGCGGGCCCGCGGTTTCCCGCGGCTCTCAAGCGTCAGGCAAGACAtgtg                      >  1:885460/1‑239 (MQ=255)

                                                                                                                                                                                                                                       ccccccccCGC‑GC‑GCGCCCAAACCACCCCCGCACCGGGCCCCTTCGGCCAATCAAGTTTTGGTTTCCTGCGGTG‑AAATCGGAAATGGTGGTGGAAAAGCTTAAGAGCCTCTTCTTCCGGCTTGAAAGAGGGGAACACACGGGCCCCG‑CCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGgcgcg      <  1:428121/235‑1 (MQ=255)

                                                                                                                                                                                                                                          gCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCG‑CCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAAc  >  2:880517/1‑239 (MQ=255)

                                                                                                                                                                                                                                          gCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGGGCAAATC‑GAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACCCGCGGCCCG‑CCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAAc  <  2:885460/239‑1 (MQ=255)

                                                                                                                                                                                                                                               ggC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCG‑CCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGc                     <  2:86498/215‑1 (MQ=255)

                                                                                                                                                                                                                                               ggC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCG‑CCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGc                     >  1:86498/1‑215 (MQ=255)

GCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACG‑CACAAAACTGTCGCGACC‑GAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGC‑GC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC‑GAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCG‑CCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAAC  >  NC_000913/4255831‑4256301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`