Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,257,903	G→T	14.6%	G55C (GGT→TGT)	dinF →	oxidative stress resistance protein, putative MATE family efflux pump, UV and mitomycin C inducible protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,257,903	0	G	T	14.6%	194.3 / 4.2	63	G55C (GGT→TGT)	dinF	oxidative stress resistance protein, putative MATE family efflux pump, UV and mitomycin C inducible protein
Reads supporting (aligned to +/- strand):  ref base G (5/48);  new base T (8/2);  total (13/50)
Fisher's exact test for biased strand distribution p-value = 1.26e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTCAGGCATTAGCCCGTACGCTGGTGCAACCGTTGCTGTTGGCGTTGGGGGCTGGGGCGTTAATTGCGCTGCTGCGTACGCCGATTATCGATCTGGCG  >  NC_000913/4257667‑4258136                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   gCTTCTCCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTg                                                                                                                                                                                                                                                    <  1:411352/237‑1 (MQ=255)          ttGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTg                                                                                                                                                                                                                                                    <  2:557301/228‑1 (MQ=255)              aGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑c                                                                                                                                                                                                                                    <  2:278738/238‑1 (MQ=255)                 gCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑g                                                                                                                                                                                                                                <  2:110924/238‑1 (MQ=255)                  cTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTTGAAAATATCTCTGGGACCGCGGTGCCGCCCGGCGTCGCGGTTTGTTTTTCTTCTCTCTTCATCAGGCTTGTCTGCCTGGCATTCCTCTCTTCATCTGATAAAGCCCTCTGGCATCTCGCCTTACCCCTGATTTTCTCCAATATCACCGTTCCGTTTCTGGGACTGGTCGATACGGCGGGGATTgtgctcttttttttgcccgg                                                                                                                                                                                                                                 >  1:631634/1‑220 (MQ=255)                           ggggTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTggg                                                                                                                                                                                                                     <  2:1038591/239‑1 (MQ=255)                                  ttCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTg                                                                                                                                                                                                             <  2:255533/239‑1 (MQ=255)                                  ttCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTg                                                                                                                                                                                                             <  2:1160933/239‑1 (MQ=255)                                       aaCGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGt                                                                                                                                                                                                       <  2:333581/239‑1 (MQ=255)                                       aaCGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGt                                                                                                                                                                                                       <  2:269479/239‑1 (MQ=255)                                       aaCGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGt                                                                                                                                                                                                       <  2:540594/239‑1 (MQ=255)                                       aaCGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGt                                                                                                                                                                                                       <  2:940006/239‑1 (MQ=255)                                       aaCGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGt                                                                                                                                                                                                       <  1:174712/239‑1 (MQ=255)                                       aaCGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGt                                                                                                                                                                                                       <  2:631634/239‑1 (MQ=255)                                                 ggCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTTGTTATC‑TTTATAGCCCC‑GGTTTTTTTGGG‑GGC‑GTGGC‑GGGTTGT‑GCGACg                                                                                                                                                                                            >  2:528512/1‑239 (MQ=255)                                                    tggaaCATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTGTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGc                                                                                                                                                                                          <  2:154162/235‑1 (MQ=255)                                                     gTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGa                                                                                                                                                                                        <  2:339942/239‑1 (MQ=255)                                                     gTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGa                                                                                                                                                                                        <  2:1220196/239‑1 (MQ=255)                                                     gTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGa                                                                                                                                                                                        <  2:374118/239‑1 (MQ=255)                                                       aaCATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGAc                                                                                                                                                                                       <  2:355065/238‑1 (MQ=255)                                                            atCTCTGAGACCGCGATGCGGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCTCTCTGGCATCTCGCGTTACCCATGATTTCCTCCAATATCACCGTTCCGGTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TGGATAG‑CCT‑GGTTTATTTGGGCGGC‑GTGGC‑GGGTGGT‑GCAACGGCGACGAGCtt                                                                                                                                                                                 <  1:128376/239‑1 (MQ=255)                                                            atCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTCATTTGGCCTA‑TTTATAA‑CCC‑GGGTTTTTTTGGGGGG‑GTGGT‑GGGTTGT‑GCTACTGCGAcccggtt                                                                                                                                                                                 >  2:336798/1‑234 (MQ=255)                                                            atCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCtt                                                                                                                                                                                 <  2:345303/239‑1 (MQ=255)                                                              ctctGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTTGTCATC‑TTGATAG‑CCC‑GGTTTATTTGTGCGGG‑GGGGC‑GGGTGGG‑GCGACGGGGACCCGCcttt                                                                                                                                                                               >  2:932703/1‑238 (MQ=255)                                                                ctGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTTGGCATCTTTTATGC‑CCG‑GGTTTTTTTGGGGGGG‑GGGGG‑GGTGGGG‑GCaaagcgcgccgccttt                                                                                                                                                                                >  2:393295/1‑223 (MQ=255)                                                                 tGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTACTTTGTCATC‑TTTGTTG‑CCC‑GGTTTTTTTTGGCGGC‑GTGGCGGGTTGGG‑GC‑ACGGCGACCCCCTTTttct                                                                                                                                                                            >  1:508547/1‑239 (MQ=255)                                                                       cgcgATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGGTAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTTGTCATC‑TTGATAG‑CCC‑GGGTTATTTGGGCGGC‑GTGGC‑GGGTGGT‑GGAACcgcggtccgcctttttttttt                                                                                                                                                                        >  2:310363/1‑220 (MQ=255)                                                                         cgATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTTGGCCTT‑TTTATAG‑CCC‑GGGTTTTTTGGGGGGG‑GTGGC‑GGTTTGG‑GGAACGGCGGccccgctttttttttttgtt                                                                                                                                                                    >  2:291501/1‑221 (MQ=255)                                                                         cgATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAAATGGTCATC‑TTGATAG‑TCC‑GGTTTATTTGTGGGGG‑GTGGG‑GGGTGGT‑GCGACAGGGAGCAGCATTCTCTTTttgtt‑g                                                                                                                                                                   >  1:597970/1‑239 (MQ=255)                                                                         cgATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGCTATTTGGCCTC‑TTTTTAA‑CCCAGGTTTTTTTGGG‑GGG‑GCGGC‑GTGTGGT‑GTGACCGCGCGCCGCCTTCTCttttttccg                                                                                                                                                                    >  1:212549/1‑233 (MQ=255)                                                                                          gcgGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGAAAATGGTCATC‑CTTTTAG‑CCC‑CGTTTTTTTTGGCGGG‑GGGGC‑GGGTGGT‑GGGACAGGGCCCAGCGTTTTCttttttttttttgtgtttttttt                                                                                                                                                      >  1:439590/1‑216 (MQ=255)                                                                                              tttgtttTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAg                                                                                                                                               <  2:461541/238‑1 (MQ=255)                                                                                              tttgtttTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAg                                                                                                                                               <  2:606710/238‑1 (MQ=255)                                                                                                    tttCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCCTC‑CTTTTAG‑CCC‑GGGTTATTTTGGCGGCGGGGGG‑GGTGGGT‑GCACCGCGGACAGGCTTTCTTTTTTTGTT‑TTTGCT‑TTTTTTCTCCTGAGcacccc                                                                                                                                         >  2:47795/1‑236 (MQ=255)                                                                                                           tctctTAATCTGGCTTGTCTGCATGGCATTCCTCACTTCATCGGATAAAGCACGCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGTACTGGTCGATGCGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCATTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGa                                                                                                                                 <  1:45914/239‑1 (MQ=255)                                                                                                                tcatcaGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTg‑cg                                                                                                                           <  1:1003412/239‑1 (MQ=255)                                                                                                                tcatcaGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGAGTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTg‑cg                                                                                                                           <  1:102825/239‑1 (MQ=255)                                                                                                                tcatcaGGCTGGTCGGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TGGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCCCCGGGCTGACTg‑cg                                                                                                                           <  1:1106309/239‑1 (MQ=255)                                                                                                                                         ccTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTc                                                                                                 <  2:692376/239‑1 (MQ=255)                                                                                                                                         ccTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTc                                                                                                 <  2:1008970/239‑1 (MQ=255)                                                                                                                                          cTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGt                                                                                                                                                                                                       <  2:596967/140‑1 (MQ=255)                                                                                                                                          cTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTCa                                                                                                <  2:837187/239‑1 (MQ=255)                                                                                                                                                                 ctGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAAATGTTTCTC‑TTTATAT‑TCC‑GGTTTATTTTGGCGGG‑GTGGCGGGTGGGT‑CGAAGGGGAACCACCTTT‑TCTTTTTTCT‑GTTTGTGTTTTTTCGCCTGGGCCCCCCCGCGCGCCCTGAGG‑CAGCCTATTGTTGCCGAAAA‑CATCAGGCGTTGTCCCGTACGCTgcgg                                                                          >  1:395689/1‑235 (MQ=255)                                                                                                                                                                       tctcGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTCAGGCATTAGCCCGTACGCTGGTGCAACCGt                                                                   <  2:970706/239‑1 (MQ=255)                                                                                                                                                                             cTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTCAGGCATTAGCCCGTACGCTGGTGCAACCGTTGCTGt                                                             <  2:1153268/239‑1 (MQ=255)                                                                                                                                                                                    aTGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTCAGGCATTAGCCCGTACGCTGGTGCAACCGTTGCTGTTGGCGtt                                                      <  2:401900/239‑1 (MQ=255)                                                                                                                                                                                    aTGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTCAGGCATGAGCCCGTACGCTGGTGCAACCGTTGCTGTTGGCGtt                                                      <  2:968816/239‑1 (MQ=255)                                                                                                                                                                                           tctcCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGCTAG‑CCC‑GGGTTTTTTTGGCGGC‑GGGGC‑GGGTGGTGGCAACGGCG‑CCACCTTTTTTTTTTTGCTGGTTTCTGTTTTT‑TGCCTTGGCCCCCCCCGGGTGGCTG‑CGCCGGGCTTTTGGGGCCAAAAAACTTCGGCCCTTATCCCGTCGCCGGGGGCACCCGTTCTGGTTGCGGTTgggggt                                                >  1:61601/1‑238 (MQ=255)                                                                                                                                                                                           tctcCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTCAGGCATTAGCCCGTACGCTGGTGCAACCGTTGCTGTTGGCGTTGGGGGCt                                               <  2:587749/239‑1 (MQ=255)                                                                                                                                                                                           tctcCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTCAGGCATTAGCCCGTACGCTGGTGCAACCGTTGCTGTTGGCGTTGGGGGCt                                               <  2:779732/239‑1 (MQ=255)                                                                                                                                                                                                     cACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTc                                                                                                 <  2:572681/179‑1 (MQ=255)                                                                                                                                                                                                        cgtcccgtgGCTGGGACGGGTCCATCCGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTCAGGCATTAGCCCGTACGCTGGTGCAACCGTTGCTGTTGGCGTTGGGGGCTGGGGCGTTAATTg                                  <  1:336798/239‑1 (MQ=255)                                                                                                                                                                                                                       aCTGGTCGATACGGCGGTAATTTGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTCAGGCATTAGCCCGTACGCTGGTGCAACCGTTGCTGTTGGCGTTGGGGGCTGGGGCGTTAATTGCGCTGCTGCGTACg                    <  2:69490/238‑1 (MQ=255)                                                                                                                                                                                                                       aCTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTCAGGCATTAGCCCGTACGCTGGTGCAACCGTTGCTGTTGGCGTTGGGGGCTGGGGCGTTAATTGCGCTGCTGCGTACg                    <  2:545891/238‑1 (MQ=255)                                                                                                                                                                                                                       aCTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTCAGGCATTAGCCCGTACGCTGGTGCAACCGTTGCTGTTGGCGTTGGGGGCTGGGGCGTTAATTGCGCTGCTGCGTACg                    <  2:968810/238‑1 (MQ=255)                                                                                                                                                                                                                        cTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTCAGGCATTAGCCCGTACGCTGGTGCAACCGTTGCTGTTGGCGTTGGGGGCTGGGGCGTTAATTGCGCTGCTGCGTACGcc                  <  2:61601/239‑1 (MQ=255)                                                                                                                                                                                                                           gTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTCAGGCATTAGCCCGTACGCTGGTGCAACCGTTGCTGTTGGCGTTGGGGGCt                                               <  2:352848/207‑1 (MQ=255)                                                                                                                                                                                                                           gTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTCAGGCATTAGCCCGTACGCTGGTGCAACCGTTGCTGTTGGCGTTGGGGGCt                                               <  2:461053/207‑1 (MQ=255)                                                                                                                                                                                                                              gATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTCAGGCATTAGCCCGTACGCTGGTGCAACCGTTGCTGTTGGCGTTGGGGGCTGGGGCGTTAATTGCGCTGCTGCGTACGCCGATTAt            <  1:528512/239‑1 (MQ=255)                                                                                                                                                                                                                                   gtcggTAATTGGGCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTCAGGCATTAGCCCGTACGCTGGTGCAACCGTTGCTGTTGGCGTTGGGGGCTGGGGCGTTAATTGCGCTGCTGCGTACGCCGATTATCGATc       <  1:339243/237‑1 (MQ=255)                                                                                                                                                                                                                                   ggcggTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCGCCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTCAGGCATTAGCCCGTACGCTGGTGCAACCGTTGCTGTTGGCGTTGGGGGCTGGGGCGTTAATTGCGCTGCTGCGTACGCCGATTATCGATc       <  2:746622/239‑1 (MQ=255)                                                                                                                                                                                                                                      ggTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTCAGGCATTAGCCCGTACGCTGGTGCAACCGTTGCTGTTGGCGTTGGGGGCTGGGGCGTTAATTGCGCTGCTGCGTACGCCGATTATCGATCTg     <  2:96220/238‑1 (MQ=255)                                                                                                                                                                                                                                        tgaTTTGTCGTC‑GTGATAG‑GCC‑GGTTTATTTGCGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTGTCGCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCGCCACCGGGCTGACTG‑GG‑CAGGCTTATGGTGCCAAAAATCCTCAGGCATTAGCCCGTACGCTGGTGCAACCGTTGCTGTTGGCGTTGGGGGCTGGGGCGTTAATTGCGCTGCTGCGTACGCCGATTATCGATCTGgc   <  1:641187/236‑1 (MQ=255)                                                                                                                                                                                                                                         aaTTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTCAGGCATTAGCCCGTACGCTGGTGCAACCGTTGCTGTTGGCGTTGGGGGCTGGGGCGTTAATTGCGCTGCTGCGTACGCCGATTATCGATCTGgcg  <  2:1052150/238‑1 (MQ=255)                                                                                                                                                                                                                                         aaTTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTCAGGCATTAGCCCGTACGCTGGTGCAACCGTTGCTGTTGGCGTTGGGGGCTGGGGCGTTAATTGCGCTGCTGCGTACGCCGATTATCGATCTGgcg  <  1:393295/238‑1 (MQ=255)                                                                                                                                                                                                                                         aaTTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTCAGGCATTAGCCCGTACGCTGGTGCAACCGTTGCTGTTGGCGTTGGGGGCTGGGGCGTTAATTGCGCTGCTGCGTACGCCGATTATCGATCTGgcg  <  2:212549/238‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   GCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGGCGGTAATTGGTCATC‑TTGATAG‑CCC‑GGTTTATTTGGGCGGC‑GTGGC‑GGTTGGT‑GCAACGGCGACCAGCTTTCTCTTTATGCT‑GTTGCTGTTTTTACGCATGAGCACCACCGGGCTGACTG‑CG‑CAGGCTTATGGTGCCAAAAATCCTCAGGCATTAGCCCGTACGCTGGTGCAACCGTTGCTGTTGGCGTTGGGGGCTGGGGCGTTAATTGCGCTGCTGCGTACGCCGATTATCGATCTGGCG  >  NC_000913/4257667‑4258136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑