New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 19794893 (0.050)98 (1.480) 62/284 0.0 97.0% intergenic (‑274/‑264) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 = 2290111 NA (NA)intergenic (‑32/+1) insH1/yejO IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system

AGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCG  >  NC_000913/2289971‑2290251
                                                                                                                                            |                                                                                                                                            
aGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCAt                                                                                                                                            >  1:363869/1‑143 (MQ=31)
 gAAAATCTCTTTTCTGGTCTGACGACGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGCCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATGTCATATCAGGGACTTGTTCGCAACTTACATa                                                                                                                                           >  2:303358/1‑143 (MQ=11)
  aataTCACTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATTATCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATaa                                                                                                                                          <  2:985567/140‑1 (MQ=255)
    aacctctTTCCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACg                                                                                                                                        <  1:703494/140‑1 (MQ=21)
    aaTCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACg                                                                                                                                        <  1:574024/143‑1 (MQ=34)
      tctcTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCt                                                                                                                                      <  2:641657/143‑1 (MQ=35)
      tctcTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCt                                                                                                                                      >  2:618431/1‑143 (MQ=35)
       ctctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTg                                                                                                                                     >  2:981930/1‑143 (MQ=35)
        tctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATCATGAACCCTGTTCTATGGCTCCAGCTGACAAACATGATCTCATATGAGGGACGTGTTCGCACATCCCATacccctct                                                                                                                                    >  2:262466/1‑136 (MQ=11)
         ggtctcgggacTGACGGCGCTTGCTGCTAGCCTCACTCGCCGCGCAGGTAAGTTGAGAACGCAGGATGAACCCTGTTCTATGGCTCCAGATCACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTa                                                                                                                                   <  2:1150066/133‑1 (MQ=11)
         ctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTCTa                                                                                                                                   >  2:147115/1‑143 (MQ=25)
          ttttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCCTATCAGGGACTTGTTCGCCCCTTCCATAACGCTGTAg                                                                                                                                  >  2:276724/1‑143 (MQ=255)
                   cTGACGGGGCGTAATGCTCAAGTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGaa                                                                                                                         <  2:1061066/143‑1 (MQ=14)
                       cGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGa                                                                                                                     >  1:71071/1‑143 (MQ=255)
                        ggCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGGTCTATGGCTCCAGATGACAAACATGATAACATATCAGGGACTTGTTCGCACCTTCCATAACCCTGTAGCCACCAGCACCGat                                                                                                                    >  2:75021/1‑143 (MQ=14)
                                 tgctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAAATATTGCTGaa                                                                                                           <  2:767851/143‑1 (MQ=255)
                                   ctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGACCg                                                                                                         >  1:938092/1‑143 (MQ=255)
                                   ctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGACCg                                                                                                         >  1:976275/1‑143 (MQ=255)
                                     gaataggcggTCTGCGAACATAAGTGGATTACTCCTGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGAc                                                                                                       <  1:831732/134‑1 (MQ=18)
                                       aTTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCCGCCATAACCCGATACCCACCCGAACAGATATTGCGGACCGAcca                                                                                                     >  2:1058206/1‑141 (MQ=14)
                                         tCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAag                                                                                                   <  1:634431/143‑1 (MQ=255)
                                         tCACTGTCGGCGAAGGTAAGGTGATGGCTCATGATGAACCCTGTGCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAag                                                                                                   <  1:553830/143‑1 (MQ=255)
                                               tCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATACCAGGGACTTGTTCGCACCTTCCATAACGCCGCAGCCACCAACACAGCTACTCAGGACCAACAAACAGAAAc                                                                                             >  2:915291/1‑143 (MQ=14)
                                                  gCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACGAGAACGGATATTGCGGAACGACAAAGAGAAACAGa                                                                                          >  1:715613/1‑143 (MQ=255)
                                                  gCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGa                                                                                          >  1:15104/1‑143 (MQ=255)
                                                           aGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTg                                                                                 >  1:485968/1‑143 (MQ=255)
                                                             ttGAGGACTCACCATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACGTTCCAGAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGAt                                                                               <  2:579150/143‑1 (MQ=25)
                                                                 tgaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCaa                                                                           >  2:423047/1‑142 (MQ=255)
                                                                     tCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGGCTTGTTCGCACCTTGCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGGCAAAGAGAAACAGAACCAGATTGATGCATTGAg                                                                       >  1:919796/1‑143 (MQ=255)
                                                                     tCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAg                                                                       >  1:996764/1‑143 (MQ=255)
                                                                     tCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCGTCTATTACGCGGTAGCCACCAGAACAGATATTGGGGAACGGCACAGAGAAGcagcagcaggaggacgcgaggcg                                                                       >  2:558373/1‑129 (MQ=17)
                                                                        gaccacgatgttttCTATGGCCCCAGATGACGACCATGCTCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCtt                                                                    <  2:740317/131‑1 (MQ=37)
                                                                         gatgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCttt                                                                   <  2:845537/143‑1 (MQ=255)
                                                                           tgaGCCCTGTTCTACGGCGCCAGATGACAAACATGGTCTCATATCAGGGACGTGTTCGCCCCTTCCATAACTCTGGAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCa                                                                 <  2:1080729/143‑1 (MQ=255)
                                                                            gaACCCTGTGCTATGGCTCCAGATGACAAACTTGATCTCCTATCAGTGACTTTTTCGCACCTTCCATAACGCTGTAGCCATCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCAt                                                                <  2:90585/143‑1 (MQ=37)
                                                                              aCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATcc                                                              >  2:656777/1‑143 (MQ=255)
                                                                               cccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGCCAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCt                                                             >  2:418072/1‑143 (MQ=255)
                                                                                ccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTa                                                            >  1:856838/1‑143 (MQ=255)
                                                                                ccTGTTCTATGGCTCCAGATGACAAACATGAACTCATATCAGTGACTTGCTCGCACCTTCCATAACGCTGCAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTa                                                            <  1:290764/143‑1 (MQ=255)
                                                                                     tCTATGGCTCCAGATGACAAACATGATCTGATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGaaa                                                       <  2:627409/143‑1 (MQ=255)
                                                                                          ggCTGCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAaattaat                                                  <  1:316836/143‑1 (MQ=255)
                                                                                           gCTGCAGATGTCAAACAGGATCGCAGAAGAGCGACGTGTTCGCACCTTCAACAACGCTGTAGCCACCAGGACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAaattaatt                                                 <  2:420925/143‑1 (MQ=255)
                                                                                           gCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAaattactt                                                 >  2:143834/1‑143 (MQ=255)
                                                                                                aGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGCTTGATGCATTGAGCTTTCATCCTATGAAATCAATTGCTGt                                            >  2:814901/1‑143 (MQ=255)
                                                                                                aGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGt                                            >  2:913889/1‑143 (MQ=255)
                                                                                                 ggtGACAAACATAATCTGATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGtt                                           <  2:71071/141‑1 (MQ=255)
                                                                                                   gtaCAAACATGAGCTCATATCAGGGACTTGACCCAGGCGTTCATGACGCAGTAACCACCAGAACAGATATTGCTGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTaa                                         <  2:506760/141‑1 (MQ=255)
                                                                                                     aCAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTaaaa                                       <  1:618431/143‑1 (MQ=255)
                                                                                                       aaaCATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGc                                     >  2:447971/1‑143 (MQ=255)
                                                                                                          caggagctcAGATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACGGATTTGGCTGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCAtt                                  <  1:532432/137‑1 (MQ=255)
                                                                                                          cATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGCCAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCAtt                                  <  2:315325/143‑1 (MQ=255)
                                                                                                           aTGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTg                                 <  1:462369/143‑1 (MQ=255)
                                                                                                              agcgcATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGAGATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGt                              <  2:689700/139‑1 (MQ=255)
                                                                                                                      tCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGaaaa                      >  1:536993/1‑143 (MQ=255)
                                                                                                                          ggACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATAcc                  >  1:1032716/1‑143 (MQ=255)
                                                                                                                              ttGTTCGCACCTTCCATAACGCTGTAGCCACCGGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGAGTACCGAAAATACCCATa              >  2:603815/1‑143 (MQ=255)
                                                                                                                                gTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGCGAAACAGAACCAGATTGCTGCATTGAGCTTTCAGCCTACGAACTTAATTGCTGTTAACAGGATGGGGTACAGCAACTAACCaaaac            >  2:335880/1‑139 (MQ=255)
                                                                                                                                   cGCACCTTCCATAACGCTGTAGCCACCAGGACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTcc         <  2:114871/143‑1 (MQ=255)
                                                                                                                                   cGCACCTTCCATAACGCTGTAGCCACCAGAACAGCTATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCCTTGAGCTTTCATCCTATGAAATTAATTGCTGTCAAACGCCTGGGCCACAGAACCTACCCACAGccct         >  2:1105681/1‑140 (MQ=255)
                                                                                                                                   cGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGATAAAGTAGATACCCATAGCTcc         >  1:713685/1‑143 (MQ=255)
                                                                                                                                       ccTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATAc     <  1:598183/143‑1 (MQ=255)
                                                                                                                                          tCCAGAACGCTGGAGCCCCCAGAACAGGTCTTGCGGTACGTCAAACAGAAACAGAGCCAGATTGATGCATGGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCg  <  2:1115108/143‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
AGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCG  >  NC_000913/2289971‑2290251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.