Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,917,404 | 0 | A | T | 26.7% | 112.7 / 10.7 | 63 | V287V (GTT→GTA) | atpG | F1 sector of membrane‑bound ATP synthase, gamma subunit |
Reads supporting (aligned to +/- strand): ref base A (29/15); new base T (0/16); total (29/34) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.46e-06 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
GCTCATTACCATTTTGCACCTCAAGAGCATCGTACACGCGCGGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGG > NC_000913/3917263‑3917539 | gCTCATTACCATTTTGCACCTCAAGAGCATCGTACACGCGCGGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGACATACCCTGTTTaaa > 1:130843/1‑143 (MQ=255) cTCATTACCATGTGGCACCTCGAGACCATCGTCCACGCGCGGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAAc < 2:549090/143‑1 (MQ=255) aCCATTTTGCACCTCAAGAGCATCGTACACGCGCGGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGACATAACCTGCGTAAACCgcggc > 1:1810/1‑143 (MQ=255) ccATTTTGCACTTCAAGATCATCGTACACTCGCGGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCgcggcg < 1:77627/143‑1 (MQ=255) ccttttggactccgagagCATCGTACCCGCGCGGAACGGCATCCTGAGGGAATTCTACGTCAACTACGTCGCCACCCACCTGGACCATTTTTACGGGACACCTCTTAAACCCTCTACGAAATAACCTGTTTAAACCGcggcgg < 2:246817/129‑1 (MQ=255) ttttGCACCTCAAGAGCATCGTACCCGCGCGGTACGGCATCCTGAGGGAAGTCGACGTCCACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGcc < 2:1119198/143‑1 (MQ=255) cACCTCAAGAGCATCGTACACGCGCGGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTGCCAGTAGCCATCTTAAATCCTCTACGCAATAACCTGCTTAAACCGCGGCGGCCCCCga > 1:437594/1‑143 (MQ=255) aCCTCAAGAGCATCGTACACGCGCGGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCAGCCCCCcag > 1:50639/1‑143 (MQ=255) tCAAGAGCATAGTACAAACGAAGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAACACTCTACGAAATAACTCGTTTAACCCGCGGCGGCCCCAGAGAca > 2:143639/1‑142 (MQ=255) aaGTGCATCGTACACGCGCGGTACGGCATCCTGAGGGAATTCGACGTCCACTACGGCGCCGATTACCTGTACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGAt < 1:753911/143‑1 (MQ=255) cATCCTACACGCCCGTTACGCCATCCTGATGGAATTCGACTTCAACTACGGCGCCGATTACCTGTACAATCTTTCCAGTATCCATCTTAAATCCTCTACGATATTTTTTTTTTATAATACGGCGGCCACCGAGACGATCTCgg < 2:721238/143‑1 (MQ=255) cGTTCACGCGTGGTTCGGTATTCTGAGGGATGTCTACGTCAACTACGGCGTCGATTACCTGGACAGTCTTTCCAGTAGCCATCTTAAGCCCTCTACGTGTTTTTTTTTTAATGATGCGGCGACCACCGAGACGATCTCGGTGa < 2:105576/143‑1 (MQ=255) tACACGCGCGGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCCGTAGCCCTCTTACATCCTCTCCCCAATACCCTCTCTCAACCCCCCCGGCCCCCGAGACCAcctccacgcag > 2:312045/1‑136 (MQ=255) cacGCGCGGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTc < 1:902240/143‑1 (MQ=255) acGCGCGGTACGGCATGCTGAGGGAATTCGACGTCAACTACGGCGCCGATGACCTGGGCAGTCTGTCCAGAAGCCATCTTAAATCCTCTCCCAAATAACCTGTATAAACCGCCGCGGCCCCCTGCACGATGTCGGCGAGCTcg > 2:503057/1‑142 (MQ=255) cgcgGTACGGCATCATGAGTGATTTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAATTTTTTTTTTAATGCTGCGGCGACCACCGAGACGATCTCGGTGAGTTCCTGa < 2:1135011/143‑1 (MQ=255) ctgTACGGCTGTCTGAGGGAATTCGACGTCATCTACGGTGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTACATCCTCTACGATTTTTTTTTTTAATGATACGGCGACCACCGAGACGATCTCGGTGAGTTCCTGAGt < 2:946306/141‑1 (MQ=255) cGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTGCCTGGACAATCCTTCCAGTAGCCTTCTGAAAACCTCTACGAATTTTTTTTTTAATGACGCGGCGGCCACCGAGACGATCTCGGTGAGTTCCTGAGTAATGc < 2:346344/143‑1 (MQ=255) cGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTACATCCTCGACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGc > 2:929484/1‑143 (MQ=255) tgCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAAGCCTCTACGAATTTTTTTGTTTATGATGCGGCGACCACCGAGACGATCTCGGTGAGTTCCTGAGTAATGCt < 2:751153/142‑1 (MQ=255) ggCATCCTGACGGACTTCCTCGTCAACTACAGCGCCGATTACCTTGACATTCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATTTCTTTTTTATGCTGCGGCGACCACCGAGACGATCTCGGTGAGTTCCTGAGTAATGCt < 2:828623/143‑1 (MQ=255) cATCCTGAGGGAATTCGACGTCAACTAAGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGCAGCTCTCGGTGAGTGCCTGAGTACTGCTgg > 2:847159/1‑143 (MQ=255) cTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTg > 1:97899/1‑143 (MQ=255) ggagtcagACGTCAACTAGGGCGCCGATCACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAg < 1:603501/136‑1 (MQ=255) ggAATTCGACGTCAACTACGACGCCAATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTCAACCGCGGCGGCCCCCGACACGATCCCGCTAAGTGCCAGCGCAATACTAGCCTCACGac > 2:551535/1‑142 (MQ=255) aaTTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGCCGAGCt > 1:664968/1‑143 (MQ=255) aaTTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCt > 1:605039/1‑143 (MQ=255) aaTTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTACAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGGTCCTGAGTAATGCTGGCCTGCCGAGCt > 1:234582/1‑143 (MQ=255) tggtgggctcttgcGGCGCGGAGTACCTGTACTATCTTTTCAGTAGTCGTCGTCAGGCCTCTGCGATTTTTTGTGTTTATGCTGCGGCGGCCACCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGATGAGCTttg < 2:260317/130‑1 (MQ=255) gACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTttgtt < 1:111608/143‑1 (MQ=255) gACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCACCGTATCTCGACCTCGGTCAGTTCCTGAGTACTGCTGGCCCGCCGAGCTttgtc > 1:261411/1‑142 (MQ=255) aCGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTttgttg < 1:918411/143‑1 (MQ=255) tCAACTACGGTGCCGATTACCTGGACAATCTTTCCAGTAGCCAGCGTAAATCCTCTACGAATTTTTTTTTTAATGCTGCGGCGGCCACCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGtat < 2:567883/143‑1 (MQ=255) tCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGGGCTCGGGAGGACTGCTGGCCGGACGCGCCttgtgggcg > 2:655001/1‑138 (MQ=255) cAAGTACTGCGCCGACTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAATTATTTTGTTAATGATGCGGCGGCCACCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGtata < 2:496049/143‑1 (MQ=255) aaCTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAGACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATAc < 1:66693/143‑1 (MQ=255) aCTACGGCGCCGCTTCCCTGGACAATCTTTCCAGTAGCCCTCGTGAATCCTCTACGAATTATCTTTTTAACGCTGCGGCGGCCACCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATAcc < 2:168507/143‑1 (MQ=255) aCTACGGCGCCGACTACCTCGACAATCGTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATAcc < 1:94991/143‑1 (MQ=255) ggCTCCGATTATCTGGACATTGTTTCCAGTATCCATCTTAAATCCTCTACGTAATTTCTTTTTTATGATGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTg < 2:503291/143‑1 (MQ=255) ggCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTg < 1:441639/143‑1 (MQ=255) ggCGCCGATTACCTAGACAATCTTTCCATTAGACATCTTAAATCCTCTACGATTTTTCTTGTTAGTGACACGGCGACCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTg < 2:927425/143‑1 (MQ=255) aTTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGctct < 1:1102548/143‑1 (MQ=255) ccTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCGTTGTTGTGTACCCAGTGCAGCGCTTTaa > 1:89044/1‑143 (MQ=255) tGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATc > 1:856591/1‑143 (MQ=255) aaTCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCGTTGTTGTATACAAACTGCAGCTCTTTAATCAGGCt > 1:682956/1‑143 (MQ=255) cTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAAGCGATCTCGGTGAGCTGCTGACGAATGCTAGCAGGACGAGATTGGATGCATACCAACTGCAGCACtttcaaacggctgcg > 2:423460/1‑131 (MQ=255) tttCCAGTAGCCATCTTAACTCCTCTACGATGTTTCCTGTTTATGCTACGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTgccg < 2:199129/143‑1 (MQ=255) tttCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTgccg > 1:787851/1‑143 (MQ=255) tttCCAGTAGCCAGCTCAAATCCTCTACGATTTTTCCTTTTTATGCTGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTgccg < 2:1045206/143‑1 (MQ=255) tCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTgccgcc > 1:767511/1‑143 (MQ=255) ccAGGAGCCAGCTTGAATCCTCTACGAAATGTCCTGTTAATGCTGCGGCGGCCACCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCa < 2:1065893/143‑1 (MQ=255) gCCATCTTGCATCCGCTACGCAATGTCCTGTTTATGCCGCGGCGTCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTgtcg < 2:632047/143‑1 (MQ=255) ttAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTcgcg > 1:610877/1‑143 (MQ=255) aaTCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCt > 1:198261/1‑143 (MQ=255) ccTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTc < 1:1027019/143‑1 (MQ=255) tctACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTGGGTGAGTTCCTGAGTAATGGTGGGCTGACGAGCTTTGTTGTATACGAACTGCCGCTCTTTAATCAGGCTGCCGCCATTGTCGGGCGCGGGTTTCAt > 1:21838/1‑143 (MQ=255) ctACGAAATAACCTGTTTAACCCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATc < 1:594061/143‑1 (MQ=255) ctACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTCGCCAGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAAGCAGGCTGCCGCCATTGTCGCTcgcgccctgcacc > 2:623443/1‑134 (MQ=255) tACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCAGTGTCGGTCGCGGCTTTCATCg > 1:411755/1‑143 (MQ=255) gAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGACGAATGCTGGCCTGGCGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGGCGGTCGCGGCTTTCATCGcca > 1:163546/1‑143 (MQ=255) gttgcttttttACACAGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCACCTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGccacca < 2:308616/135‑1 (MQ=255) aaCCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGGGCTCCTGAGCATTACTGGCCAGACGAGCTATGGTGTATACCAACTGCAGCACTGTAATCAGGCGGCCGCCATTGTCGGTGGCGGCGGCCAACGccaccaca > 2:801569/1‑141 (MQ=255) cTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTGATAGCCACCATAAgg > 1:823079/1‑143 (MQ=255) cTGTTTAAACCGCCGCCCCCCCCGACACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACgg < 1:545010/143‑1 (MQ=255) | GCTCATTACCATTTTGCACCTCAAGAGCATCGTACACGCGCGGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGG > NC_000913/3917263‑3917539 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |