Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,824,237 G→A M613I (ATG→ATA spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,824,2370GA97.1% 181.1 / NA 72M613I (ATG→ATAspoTbifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base G (2/0);  major base A (42/25);  minor base C (1/1);  total (45/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACG  >  NC_000913/3824097‑3824375
                                                                                                                                            |                                                                                                                                          
cTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGGAGAGTTTATCGc                                                                                                                                          >  1:922987/1‑143 (MQ=255)
   aTCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCtgt                                                                                                                                       >  1:243295/1‑143 (MQ=255)
    tCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGCAGTTTATAGCtgtg                                                                                                                                      >  1:74613/1‑143 (MQ=255)
       tcTTTGCGATATGCTCCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGaa                                                                                                                                   <  1:303318/142‑1 (MQ=255)
         ttggCGAAATGCTGCCGCCCTATCCCGGGCGACCCGAGTATGGCCCACGTCAGCCCGGGTAAAGGGCGGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTAGAGCTGTGGAATg                                                                                                                                 <  1:13388/140‑1 (MQ=255)
              gAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTCCCAGAACGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATa                                                                                                                            >  1:716916/1‑143 (MQ=255)
              gAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATa                                                                                                                            >  2:739369/1‑143 (MQ=255)
                aaTGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATaaa                                                                                                                          <  1:539548/143‑1 (MQ=255)
                  tgctgcCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGGGATCCACCATGAATCCTGCCGTAATATCCGTGGTTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAaga                                                                                                                        >  1:107098/1‑143 (MQ=255)
                     tgcCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGCGCCAGAGAAGTGTATAGCTGTGGACTGGGATAAAGAGAc                                                                                                                     >  2:692939/1‑143 (MQ=255)
                            cTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAg                                                                                                              >  2:67771/1‑143 (MQ=255)
                            cTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAACGGGATAAAGAGACGGCGCAg                                                                                                              >  1:165112/1‑143 (MQ=255)
                              aTTGCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGGGATCCACCATGAATCCTGCCGTAATATCCGAGGCGACCAGAAAGCGCCAGAGAAGTTTATAGCTGGGGAGTGGGATAAAGAGACGGCGCAGGa                                                                                                            >  2:43076/1‑143 (MQ=255)
                              aTTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGACAGAGCCAGAGAAGTTTATGGATGTGGAATGGCATAAAGAGACGGCGCAGGa                                                                                                            >  2:728178/1‑143 (MQ=255)
                                      cGACCCGATTACCGCCCACGTCAGCCCCCGTAACAACCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTtcatca                                                                                                    <  1:309920/143‑1 (MQ=255)
                                          ccGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGGCGGCGCAGGCGGTCATCACCGa                                                                                                >  2:1596/1‑143 (MQ=255)
                                          ccGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCGGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCCCCgg                                                                                                >  2:366784/1‑142 (MQ=255)
                                          ccGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGATAGGGGCTGTTTAATGGGATAAAGAGACGGCGCAGGAGGTCATCACCGa                                                                                                >  2:991880/1‑143 (MQ=255)
                                            gATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGaaa                                                                                              >  1:872318/1‑143 (MQ=255)
                                                 tCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCGGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGGGGGAGTTCGTCCCCGGGATCAAg                                                                                         >  2:644678/1‑143 (MQ=255)
                                                     ccACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTgg                                                                                     >  2:172564/1‑143 (MQ=255)
                                                      cACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGga                                                                                    <  2:727993/143‑1 (MQ=255)
                                                        cGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATACAGCGACGGCGCCGGAGTTCAGCACCGAAATCACGGTGgaga                                                                                  >  2:959390/1‑143 (MQ=255)
                                                              ccccGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCa                                                                            >  1:463263/1‑143 (MQ=255)
                                                              ccccGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGAGCATAGCTGTGGAATGGGATAAAGAGACGGCACAGGAGTTCATCACCGAGATCAACGGGGCGATGTTCa                                                                            >  2:624987/1‑143 (MQ=255)
                                                                cgggTAAAGGTCTGGGGATGCAGCAGGGGGCCTGGCGTAATATCCGTGGGTAGGAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAAGGGGATAAAGAGACGGCGCAGGAGTTCAGCACCGAAATCAAGGTGGAGATGTTCAat                                                                          <  2:163468/141‑1 (MQ=255)
                                                                ccGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTCTAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAat                                                                          >  1:613613/1‑143 (MQ=255)
                                                                      aaGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAg                                                                    <  1:343445/143‑1 (MQ=255)
                                                                       aGGTGTGGTGATGCATCATGGATCCTGCCGTAATATCCATGGCTACCAGAAAGAGCCAGAGAAGTGTATAGCTGTGGAATGGGATAAAGAGATGGCACAGTAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAgg                                                                   <  2:939421/143‑1 (MQ=255)
                                                                        ggTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAggg                                                                  <  1:67771/143‑1 (MQ=255)
                                                                         gTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAACCAGAGAAGTTCATAGCCGTGGAATGGGATAAAGAGCCGACGAAGGACTTACGCACCGAAATCAAGGTGGAGAAGTGCAATCATCACGGt                                                                 >  2:343070/1‑143 (MQ=255)
                                                                           cTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGCTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGCAATCAAGGTGGAGCTGTTCAATCATCAGGCTgc                                                               >  2:798453/1‑143 (MQ=255)
                                                                                gATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTgg                                                          <  1:579499/143‑1 (MQ=255)
                                                                                gATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGAGAAAGAGACGGCGCAGGAGTTCATCACAGAAATCAAGGTGGAGATGGTGAATCATCAGGGTGCGCTgg                                                          >  1:69343/1‑143 (MQ=255)
                                                                                 aTCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGc                                                         >  2:119389/1‑143 (MQ=255)
                                                                                    caccaTGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCaaa                                                      >  2:203351/1‑143 (MQ=255)
                                                                                     accaTGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGAGGAGATGTTCAATCATCAGGGTGCGCTGGCAAAc                                                     >  2:91338/1‑143 (MQ=255)
                                                                                      ccaTGAATCCTGCCGTACTCTCCGTGGCTACCAGCAAGAGCCAGAGAAGTTAATAGCTGCGGCATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGGTGTTCACTCATCCGGGTGCGCTGGCAAAcc                                                    >  2:445895/1‑143 (MQ=255)
                                                                                      ccaTGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGCGAAGTCTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCACGGCGGCGCAGTGCAATCAGCAGGGTGCGCCGGCAAccc                                                    >  2:200214/1‑143 (MQ=255)
                                                                                       cgtGACTCCAGCCGTAATATCCGTGGGTACCAGAAATAGCCAGAGAAGTTTAGAGCTGTGGAATAGGATAAAGAGACGGCGCATGAGTTCATCACCGAAATCATGGTAAGGATGCTCACCCCTCAGGGTGCGCTGGCAAACCt                                                   <  2:249352/141‑1 (MQ=255)
                                                                                       caTGAGTCCCGCTGTACTATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCt                                                   <  2:457926/143‑1 (MQ=255)
                                                                                         tGAATCCGGCCGTAATATCCGTGGCAACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGCGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGa                                                 <  2:542360/143‑1 (MQ=255)
                                                                                          gAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGAc                                                <  2:472349/143‑1 (MQ=255)
                                                                                             ttcggCCGGAAGAGCCGTGGCTACCAGAGAGAGTCAGAGAAGTTTAGAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGAcggc                                             <  1:590369/139‑1 (MQ=255)
                                                                                                tGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACggcggc                                          >  1:874729/1‑143 (MQ=255)
                                                                                                 gCCGTAATATCCGTGGCTACCCGAAAGAGCCAGAGAAGTTCAGAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCCAAATCAAGGTGGAGATGTTCAACCATCAGGGTGCGATGACAAACCTGACGCCGGCa                                         >  2:139608/1‑143 (MQ=255)
                                                                                                 gCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAAGTGGAGATGTTCAATCACCCGGGTGCGCCGGCAAACCTGCCGGCCGCa                                         >  2:139606/1‑143 (MQ=255)
                                                                                                   cGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAAt                                       <  1:23319/143‑1 (MQ=255)
                                                                                                      aagatCCGCGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGTGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTaa                                    <  1:652698/140‑1 (MQ=255)
                                                                                                          tCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGGCGGCGGCAATTaccacc                                >  2:545870/1‑143 (MQ=255)
                                                                                                          tCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGGGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAAcacc                                >  2:545832/1‑143 (MQ=255)
                                                                                                                  tACCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTc                        >  1:824941/1‑143 (MQ=255)
                                                                                                                   aCCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCg                       >  2:318955/1‑143 (MQ=255)
                                                                                                                   aCCAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACATGACGGCGGCCATTAACACCACGGCTTCg                       >  2:245330/1‑143 (MQ=255)
                                                                                                                     cAGAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGaa                     <  1:1596/143‑1 (MQ=255)
                                                                                                                       gAAAGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAata                   <  1:544584/143‑1 (MQ=255)
                                                                                                                         aaGAGTCAGAGATGTTTATTGCTGTGTAATGGTATAAAGATACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATAtt                 <  1:443791/143‑1 (MQ=255)
                                                                                                                         aaGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATAtt                 <  1:739369/143‑1 (MQ=255)
                                                                                                                         aaGAGCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATAtt                 <  1:373128/143‑1 (MQ=255)
                                                                                                                           gagCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCa               <  2:95972/143‑1 (MQ=255)
                                                                                                                            agCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCaa              >  2:775743/1‑143 (MQ=255)
                                                                                                                             gCCAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCATGGTGTAGATGTTTTATCATCAGTGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCaaa             <  1:590169/143‑1 (MQ=255)
                                                                                                                              ccAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCCAGGTGGAGATGTTCAATCATCAGGGTGAGCTGGAAAACCTGACGGAGGCAATTAACACCACGACTTCGAATATTCAAAg            >  1:143417/1‑143 (MQ=255)
                                                                                                                              ccAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAg            >  2:865833/1‑143 (MQ=255)
                                                                                                                              ccAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCGGGCAAACCTGACGGCGGCAATTCACACCACGACCCCGCCTATTCAAAg            >  2:117667/1‑143 (MQ=255)
                                                                                                                              ccAGAGAAGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGATCATCACCGAAATCAAGGTGGAGATGTTAAATCACCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAAGACGACGACTTCGAATATTCACAg            >  1:375691/1‑143 (MQ=255)
                                                                                                                                 gagaAGTTTCTCGCTGGGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGCTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGttt         <  2:924073/143‑1 (MQ=255)
                                                                                                                                    aaGTTTATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGaa      <  1:823277/143‑1 (MQ=255)
                                                                                                                                       tttATAGCTGTGGAATGGGATAAAGAGACTGCGCAGGGGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATAc   >  2:418365/1‑143 (MQ=255)
                                                                                                                                       tttATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATAGTCAAAGTTTGAATAc   >  1:987460/1‑143 (MQ=255)
                                                                                                                                       tttATAGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGAGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACAACGACTTCGAATCGTCAAAGTTTGAAtgc   >  2:213803/1‑141 (MQ=255)
                                                                                                                                        ttATAGCGGTGGAATGGGCTAAAGAGAGGGCGCAGGAGTTCACAACCCAAGTCAAGGTAAAATTGAGAATTGTTTGTGGTGGGTTGCCAAACCTGACGGCGGCAATTAATACCTCGATTTCGAATATTCAAAGTTTGAATACg  <  2:243295/143‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                          
CTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGCTACCAGAAAGAGCCAGAGAAGTTTATGGCTGTGGAATGGGATAAAGAGACGGCGCAGGAGTTCATCACCGAAATCAAGGTGGAGATGTTCAATCATCAGGGTGCGCTGGCAAACCTGACGGCGGCAATTAACACCACGACTTCGAATATTCAAAGTTTGAATACG  >  NC_000913/3824097‑3824375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: