| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,160,112 | G→T | H226Q (CAC→CAA) | sthA ← | pyridine nucleotide transhydrogenase, soluble |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,160,112 | 0 | G | T | 96.7% | 163.7 / NA | 60 | H226Q (CAC→CAA) | sthA | pyridine nucleotide transhydrogenase, soluble |
| Reads supporting (aligned to +/- strand): ref base G (0/2); new base T (28/30); total (28/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATA > NC_000913/4159974‑4160250 | ggTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGAt < 2:967944/143‑1 (MQ=255) cGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGga < 2:892931/143‑1 (MQ=255) gTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCCCTGTTCCCGAATTGATAGgagagag > 1:251519/1‑143 (MQ=255) tAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTCGTGACGAATCACTACGCCCCTGTTCCAGAAGTGATAGGAGAGAGAATCTGa < 2:603410/143‑1 (MQ=255) caggcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGGCAtct > 1:996416/1‑143 (MQ=255) ggcagTCAGCTTTCAGTTTATTACCCGACTACAGATGCATGATCACACCATCCTCACAGCCTTCGATATTCTCATACTCTCCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGACAGGAGAGAGAATCtacaatccct > 2:667336/1‑134 (MQ=255) gTCAGCTTTCAGTTTTTTACCCGACTTAAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGAAATCTATTGaa > 2:466974/1‑142 (MQ=255) gTCAGCTTTCACTTTCTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGAt < 1:830743/143‑1 (MQ=255) aGCTTTCAGTTTTTTACCCGACTGCAGATGCATGAGCACACCAGCGTCACAGCCTTCGATCTTCTCGTACGCTTCGTTGGGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCga < 1:472665/143‑1 (MQ=255) gtgggacgttgttTACCCGTCTGCAGTTGCATGATCGCACCGTCGGCACAGCCTTCGAGCTTCTCGTACTCTTGGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACAGCTCTTGATCtag < 2:231550/136‑1 (MQ=255) ggTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGc < 2:961848/142‑1 (MQ=255) aGTTTTTTACCCGACTTCAGATCCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGc > 1:608140/1‑143 (MQ=255) ttttttCCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCcag < 2:77841/143‑1 (MQ=255) ttttACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCCCTGTTCCAGAATTGATAGGAGCGAGAATCTGACACCTCTTGCTCGAGCACCGCcagc > 1:443049/1‑143 (MQ=255) ccGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATCGCAGAGAGAATCTGGCTTCCCTTGATCGAGAAATGCGAGCAGGCGa > 1:98248/1‑143 (MQ=255) ccGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGa > 2:16693/1‑143 (MQ=255) cGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGAt < 1:749475/143‑1 (MQ=255) gACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTAATAGGAGAGAGAATCTGACAGATCTTGATCGAGAAATGCCAGCAGGCGATc > 1:845955/1‑143 (MQ=255) ttCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATcgcg > 1:55240/1‑143 (MQ=255) tCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCgg < 1:69635/143‑1 (MQ=255) tCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCgg < 1:701864/143‑1 (MQ=255) gATGCGTGATCACACCATCGTCACAGCCTTCGATCTTCTCGTAATCTTTGGTGTGACGAGTCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGgtg < 1:43380/143‑1 (MQ=255) aTGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGGCACAGTTACAGAATTCATAGGAGAGAGAATCTGACACCTCTTGATCGAGAAATGCCAGCAGGCGGTCGCGGgtgt > 2:624118/1‑143 (MQ=255) aTGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGGTGAt > 1:254727/1‑143 (MQ=255) gctCGTCCGAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATGTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACt < 1:417097/141‑1 (MQ=255) tCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACttt < 1:910936/143‑1 (MQ=255) tCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACttt < 1:277036/143‑1 (MQ=255) gcgtgagcgCCTTCGATCTTCGCGTATTCTTCGTTGTGACGAATCACTACGCCTCTGTTCCAGAATTGATAGGCGGGAGATTCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACttt < 2:161954/135‑1 (MQ=255) cgccccaGCCCTCGATCTTCTCGCTCTCTTCGTTGTGACGAATCACGACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTATTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACtttt < 1:144325/138‑1 (MQ=255) cGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCCGGCGATCGCGGGTGTTGGTCAGATCCACtgtt > 1:617107/1‑143 (MQ=255) gtggcaGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTa < 1:78309/139‑1 (MQ=255) cacaGCCGTCGGTGTGCTCGTACTCTTCGTTGGGACGAATCACGACGCCACTGTTCCAGAATGGATAGGCGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCTCTTTTACa < 2:1012805/143‑1 (MQ=255) caGCCTGCGATCTTCTCGGTCTCTTCGGCGGGGCTGATCCCTACGCCACTGCTCCAGAATTGATAGGAGATAGAATCTGACAGCTCTGGAGCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATc < 2:1041835/143‑1 (MQ=255) cTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGTGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATa < 2:352112/143‑1 (MQ=255) ttCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCGGAATTGATAGGAGAGGGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCGGAGCGACTGTTACAGCCATAc > 1:445547/1‑143 (MQ=255) ttCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATAc > 2:1137859/1‑143 (MQ=255) ttCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGGTGATCAGATCCCCTTTTACATCCAtgc > 1:563106/1‑141 (MQ=255) ttCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGACAATCTGACATCTCTTGATCGAGGAATGCCAGCAGGTGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATAc > 2:580793/1‑143 (MQ=255) tCGATCTTCTTGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATAcc > 1:559746/1‑143 (MQ=255) ctcGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCCGATCCACTTTTACATCCATACCGCGGAAgg > 1:204165/1‑142 (MQ=255) cGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGACCCACTTTTACATCCATACCGCGGAAGCTc > 1:446228/1‑143 (MQ=255) tctTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGc < 1:91690/143‑1 (MQ=255) ttCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCat > 2:193426/1‑143 (MQ=255) ttCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCAACTTTTACATCCATACAGCGGAAGATCGACGCat > 2:573323/1‑143 (MQ=255) tCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGAGTGTAGATCAGATACACTAATACAACAATACCGCGGAAGATCGACGCata > 1:1054296/1‑143 (MQ=255) tCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGCGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCata < 2:520133/143‑1 (MQ=255) tgACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATAGAGAAATGCAAGCAGGCGATCGCGGGTGTTGATCAGAACAACTTTTGCATCCATACCGCGGAAGATCGAAGCATAttacca > 2:340281/1‑139 (MQ=255) gcGAATCACTACGCCCCTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGc < 2:996416/142‑1 (MQ=255) aCGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGc < 2:579585/143‑1 (MQ=255) aCGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGc < 1:812338/143‑1 (MQ=255) cGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGcc < 1:1103938/143‑1 (MQ=255) aaTCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCATGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGa < 2:743012/143‑1 (MQ=255) gcACTACGTCACTGTTCCAGAATTGATAGGACAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGCTGATCAGATCCACTTTTCCCTCCATACCGCGGAAGATCGACGCATATTCACAGCCGATc < 1:231834/142‑1 (MQ=255) cACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCa < 1:16693/143‑1 (MQ=255) tACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGCTCACTc > 2:1039690/1‑143 (MQ=255) ccACTGTTTCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGc < 2:1045983/143‑1 (MQ=255) tGTTCCATAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCa < 1:1046771/143‑1 (MQ=255) ccAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATAGAGGAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTGTTACATCCATACCGCGGGAGATCGACGGATATTGACGACCGATCACtacggccccgtgga > 2:631788/1‑130 (MQ=255) aGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATGCCGCGGAAGATCGAGGCATATTCACAGCGGATCACTCCAGCACCATAGATa > 1:533543/1‑143 (MQ=255) aGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGaca > 1:994433/1‑141 (MQ=255) | GGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATA > NC_000913/4159974‑4160250 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |