Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499–1300697	1300697	1–1199	64 [0]	[0] 63	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	61 (1.410)	47/276	0.0	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

AACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGG  >  NC_000913/1300559‑1300839                                                                                                                                            |                                                                                                                                              tacacttagttataaggatttccttaactgctcctcctcaccatcatgttattttcgccacatcataatcctcggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataa                                                                                                                                            <  1:189915‑M2/4‑1 (MQ=255)    agttagttataaggattgccctaactgcttctcctcgccatcatgttattttcgcaacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataataa                                                                                                                                         <  2:527276‑M2/7‑1 (MQ=255)                tgatttcctgaactgcttctcctcgccatcttgttattttcgccacatcataatcctgggcttgctgaagaagaattggaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaaa                                                                                                                             <  2:730823‑M2/19‑1 (MQ=255)                ggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaaa                                                                                                                             <  2:503643‑M2/19‑1 (MQ=255)                     tccttatctgcttgtcctggccatgatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                        <  2:775889‑M2/24‑1 (MQ=255)                     tccttaactgcttctcctcttcattatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                        <  1:79805‑M2/24‑1 (MQ=255)                       cttaactgcttctcctcgtcatgaggttagtttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgt                                                                                                                      <  2:505645‑M2/26‑1 (MQ=255)                       cttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattagttccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgt                                                                                                                      >  1:51880‑M2/118‑143 (MQ=255)                       cttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATATTAATCAATAGTTAAATTATggt                                                                                                                      >  1:564348‑M2/118‑143 (MQ=255)                       cttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgt                                                                                                                      <  1:624300‑M2/26‑1 (MQ=255)                          aactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCa                                                                                                                   <  1:420304‑M2/29‑1 (MQ=255)                          aactgcttctcctcaccatcatgttatcttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCa                                                                                                                   <  1:508022‑M2/29‑1 (MQ=255)                           actgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAt                                                                                                                  >  1:75373‑M2/114‑143 (MQ=255)                               cttctcctcaccatcatgttattttcgccacatcctaatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCa                                                                                                              <  1:543939‑M2/34‑1 (MQ=255)                               cttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCa                                                                                                              <  1:201697‑M2/34‑1 (MQ=255)                                  ctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCacta                                                                                                           >  2:94969‑M2/107‑143 (MQ=255)                                  ctcctcaccatcatgttattttcgcaacatcataatcctgggcttgctgaagaataattgaaatgatattattaattcaactgcctttggtataggaaagtgctaaATAATAATCAATGGTTGAATTATTGGGCAAttcagtg                                                                                                           >  2:635431‑M2/107‑140 (MQ=255)                                   tcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCactac                                                                                                          >  2:75274‑M2/106‑143 (MQ=255)                                     ctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTg                                                                                                        >  1:183785‑M2/104‑143 (MQ=255)                                      tcgccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTgg                                                                                                       <  2:484863‑M2/41‑1 (MQ=255)                                      tcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTgg                                                                                                       >  1:132862‑M2/103‑143 (MQ=255)                                         ccatcatgtgattttcgccacatcataatcctgggcttgctgaagaaaaatagaaagtacctgattttttccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAAc                                                                                                    <  1:359420‑M2/44‑1 (MQ=255)                                          catcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACt                                                                                                   >  1:781621‑M2/99‑143 (MQ=255)                                            tcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGt                                                                                                 >  2:499893‑M2/97‑143 (MQ=255)                                               tgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAAt                                                                                              <  1:464287‑M2/50‑1 (MQ=255)                                               tggtattttcgccacatcataatactgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAAt                                                                                              <  2:185766‑M2/50‑1 (MQ=255)                                                         gccacatcataatcctgggcttgctgaagaataattgaaatgacattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAt                                                                                    <  1:550389‑M2/60‑1 (MQ=255)                                                            acatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactacctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATATGAAaagaaaga                                                                                 >  2:485‑M2/81‑143 (MQ=255)                                                              atcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtaaaggaacatgcaaaATAATAATCAATTCTTAAATTATTGTGCATTTCACTAATGGAACTGTAATCAGAAAAGATAGACa                                                                               >  2:50875‑M2/79‑143 (MQ=255)                                                                cataatcctgggctagctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAAAAAAGATAGACATg                                                                             >  2:209500‑M2/77‑143 (MQ=255)                                                                     tcctcggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAg                                                                        <  1:476617‑M2/72‑1 (MQ=255)                                                                               gctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAtctctc                                                              >  1:498213‑M2/62‑142 (MQ=255)                                                                                ctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAt                                                             <  2:592240‑M2/83‑1 (MQ=255)                                                                                ctgaagaataattgaaatgatattattaattccactgccgttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTGACAACTGGAACTGTAATCAGAAAAGATAGACATGCTTACCCAATCTCTAt                                                             >  2:309001‑M2/61‑143 (MQ=255)                                                                                 tgaagaataagtgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAtt                                                            <  2:362211‑M2/84‑1 (MQ=255)                                                                                   aagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTa                                                                         >  1:744669‑M2/58‑128 (MQ=255)                                                                                   aagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTa                                                                         <  2:744669‑M2/71‑1 (MQ=255)                                                                                           ttgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGACAATCTCTATTAGattggatt                                                  >  2:156285‑M2/50‑143 (MQ=255)                                                                                             gaaattatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGCGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaattga                                                <  1:457616‑M2/96‑1 (MQ=255)                                                                                             gaaatgatattattaattccacttcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaattga                                                <  2:492320‑M2/96‑1 (MQ=255)                                                                                             gaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaattga                                                <  2:694019‑M2/96‑1 (MQ=255)                                                                                              aaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGAttgaattgaa                                               <  1:552782‑M2/97‑1 (MQ=255)                                                                                                  gatataattaactcctctgcctttggtttaggaaagtgctaaATAATAATCAATTTTTAAATTATTGTGCATTTCACTACTGGAACTTTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAt                                           <  2:435377‑M2/101‑1 (MQ=255)                                                                                                     attattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTCGCCAATCTCTATTTGATTGAATTGAAACTtgtt                                        >  2:105157‑M2/40‑143 (MQ=255)                                                                                                        attaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgt                                     <  1:517282‑M2/107‑1 (MQ=255)                                                                                                        attaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgt                                     >  2:54349‑M2/37‑143 (MQ=255)                                                                                                        attaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgt                                     >  1:557625‑M2/37‑143 (MQ=255)                                                                                                        agtaattccactgtcgttgggagaggaaagtgctagATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgt                                     <  1:348973‑M2/107‑1 (MQ=255)                                                                                                          taattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAGGATGTTTGTTa                                   >  2:120279‑M2/35‑143 (MQ=255)                                                                                                              tccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAACCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGc                               <  1:257885‑M2/113‑1 (MQ=255)                                                                                                                cactgcctttggtagaggaaagagctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCAt                             <  2:356118‑M2/115‑1 (MQ=255)                                                                                                                 actgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATg                            <  1:664751‑M2/116‑1 (MQ=255)                                                                                                                  ctgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAAGTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATgg                           <  1:557638‑M2/117‑1 (MQ=255)                                                                                                                    gcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGAt                         <  2:189071‑M2/119‑1 (MQ=255)                                                                                                                    gcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGAt                         <  1:430173‑M2/119‑1 (MQ=255)                                                                                                                    gcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTACATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGAt                         <  2:403589‑M2/119‑1 (MQ=255)                                                                                                                      ctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGc                       >  1:113884‑M2/23‑143 (MQ=255)                                                                                                                        ttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCaa                     <  2:388460‑M2/123‑1 (MQ=255)                                                                                                                            tagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCta                 <  1:260938‑M2/127‑1 (MQ=255)                                                                                                                                  aaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGAtt           <  1:47364‑M2/133‑1 (MQ=255)                                                                                                                                   aagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAGCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTc          <  2:480605‑M2/134‑1 (MQ=255)                                                                                                                                        ctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGAATGCAAGCTATATATTCTGATa     >  2:765033‑M2/5‑143 (MQ=255)                                                                                                                                           aATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGCTGTTTGTTAAGGCACGGATGCAAGCTATAAATTCTGATACgg  >  1:654893‑M2/2‑143 (MQ=255)                                                                                                                                            |                                                                                                                                              AACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGG  >  NC_000913/1300559‑1300839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑