Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,978,503	Δ776 bp		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1978503–1979270	1979278	9–776	58 [0]	[1] 59	insB1–insA	insB1, insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1978502	0 (0.000)	53 (1.260)	44/268	0.0	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

GCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTG  >  NC_000913/1979142‑1979417                                                                                                                                          |                                                                                                                                           tttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAg                                                                                                                                       <  2:639249‑M2/6‑1 (MQ=255)  ttacacttatctgagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGt                                                                                                                                      <  1:544689‑M2/7‑1 (MQ=255)      acttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatccgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATa                                                                                                                                  >  2:742616‑M2/133‑143 (MQ=255)       cttatctaagatttttcctaaatcgacgcacctgcatcggcgattgcagtcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATAc                                                                                                                                 <  2:550371‑M2/12‑1 (MQ=255)              aagatttttcctaaatcgacgcacctccactcgtcacgacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttgtt                                                                                                                          <  1:323620‑M2/19‑1 (MQ=255)              aagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttgtt                                                                                                                          >  1:538871‑M2/125‑143 (MQ=255)              aagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggagggggcctgcgagtttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttgtt                                                                                                                          >  1:292889‑M2/125‑143 (MQ=255)               agatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttgttt                                                                                                                         <  1:726028‑M2/20‑1 (MQ=255)               agattgtgcctaaatcgacgaaactgtgttcgtcactacacgcacatacaacgtaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttgttt                                                                                                                         <  2:33823‑M2/20‑1 (MQ=255)                 attttgcctaaatcgacgaaactgcattcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttgttttg                                                                                                                       <  2:715254‑M2/22‑1 (MQ=255)                  tttttcctaaatcgacgcaactgtacgcgtcactacacgcacatacaacggaggggggctgcgattttaaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                                                                      <  1:338650‑M2/23‑1 (MQ=255)                  tttttcctaaaccgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataaggcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                                                                      <  1:426323‑M2/23‑1 (MQ=255)                         taaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataactgTATAAGCCATACTTTTGTTTCGGGTGTAtt                                                                                                               >  1:377491‑M2/114‑143 (MQ=255)                           aatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTc                                                                                                             >  1:665285‑M2/112‑143 (MQ=255)                            atcgacgcaactgtactcgtcactaaacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacatacatgTATAAGTCACACTTTTGCTTTCGGTGTACTTCa                                                                                                            >  2:768748‑M2/111‑143 (MQ=255)                              caacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAAt                                                                                                          <  1:171201‑M2/35‑1 (MQ=255)                                acgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacatcaatgTATAAGTCATACTTTTGTTTTGTGTGTATTTCAATCt                                                                                                        >  2:733554‑M2/107‑143 (MQ=255)                                  gcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGt                                                                                                      >  1:380711‑M2/105‑143 (MQ=255)                                       tgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaacgTATAAGTCATACTCTTCTTTTGCATGTATCTCAATGTGTTtaaa                                                                                                 >  2:729653‑M2/100‑143 (MQ=255)                                        gtactctgcgctacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacagaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTaaaaa                                                                                                <  1:720218‑M2/45‑1 (MQ=255)                                        gtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTaaaaa                                                                                                <  1:768605‑M2/45‑1 (MQ=255)                                        ggactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTaaaaa                                                                                                <  1:158079‑M2/45‑1 (MQ=255)                                          actcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGt                                                                                              >  2:94099‑M2/97‑143 (MQ=255)                                            tcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaccatacatgTATAAGTCATACTTTGATGTTGTGTCGATTTCCATCTGTTAAAACGttt                                                                                            >  2:464907‑M2/95‑143 (MQ=255)                                                  ctacacgcacatacaacggagtggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgct                                                                                      >  2:671935‑M2/89‑143 (MQ=255)                                                        gcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgctacgcta                                                                                <  1:426185‑M2/61‑1 (MQ=255)                                                          acatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagc                                                                              <  1:254093‑M2/63‑1 (MQ=255)                                                          acatacaacggaggggggcggcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagc                                                                              <  1:495436‑M2/63‑1 (MQ=255)                                                                 acggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGATTTGGGTGTATTTCAATCTGTTAAAAAGGTTTTCGCtacgattgcacgtcaa                                                                       >  2:81828‑M2/74‑137 (MQ=255)                                                                    gaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAAt                                                                    <  1:600955‑M2/73‑1 (MQ=255)                                                                    gacgggggttgcgactttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAAt                                                                    <  1:481740‑M2/73‑1 (MQ=255)                                                                     aggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTCCGCTAGCAAGCAAAAATg                                                                   >  1:277368‑M2/70‑143 (MQ=255)                                                                     aggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTCTATTTCACTCTGTTAAAAAGTTTTTAGCTCGTCTGGCAAGCAAAAATg                                                                   >  1:567825‑M2/70‑143 (MQ=255)                                                                       gggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGaa                                                                 >  1:444227‑M2/68‑143 (MQ=255)                                                                        ggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGaaa                                                                <  2:100584‑M2/77‑1 (MQ=255)                                                                             tgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAgg                                                            >  1:782479‑M2/62‑142 (MQ=255)                                                                             tgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAgg                                                            <  2:782479‑M2/81‑1 (MQ=255)                                                                                gattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaata                                                        >  1:113115‑M2/59‑143 (MQ=255)                                                                                           atgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGGCATACTGTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATggg                                             >  1:494574‑M2/48‑143 (MQ=255)                                                                                                   tgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTgcg                                     >  2:699904‑M2/40‑143 (MQ=255)                                                                                                     cagagctcataaacccctcaattactgaacatacatgTATACGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTGAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGTATGTTGCGca                                   <  2:648617‑M2/106‑1 (MQ=255)                                                                                                       gatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGcaca                                 <  1:742616‑M2/108‑1 (MQ=255)                                                                                                        atcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGTTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGTAATAATCGAAATTGGATGTTGCCCACAg                                >  2:602503‑M2/35‑143 (MQ=255)                                                                                                          cacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTTTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTc                              <  1:781438‑M2/111‑1 (MQ=255)                                                                                                          cacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTc                              >  1:725804‑M2/33‑143 (MQ=255)                                                                                                           atacaaaacacttaattacttagcataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGGTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCa                             <  2:470177‑M2/112‑1 (MQ=255)                                                                                                             acaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCaaa                           >  1:719802‑M2/30‑143 (MQ=255)                                                                                                                 aactctcaattgcttaacattaatgTATAAGTCATACTGTTGTTTTGGGTGTATTTCAAGCTGTTAAAAAGTTGTACGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATaa                       <  2:454530‑M2/118‑1 (MQ=255)                                                                                                                         attacttaacataaatgTATAAGTCATACTTTTTTTGTGGGTGTATTTCAATCTGTTAAACAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGt               <  2:326796‑M2/126‑1 (MQ=255)                                                                                                                             cttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAACATAACTCACCGTAaat           >  1:152161‑M2/14‑143 (MQ=255)                                                                                                                             cttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGAGCACATGCAAAATAACTCACCGTAaat           >  2:582305‑M2/14‑143 (MQ=255)                                                                                                                              ttaacataaatgTATAAGTAATAATTTTGTTTTGTGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGTAAGTAAAAATGAAACAGGAATAATCTAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAaata          <  2:65000‑M2/131‑1 (MQ=255)                                                                                                                                 acataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAatc       <  2:444227‑M2/134‑1 (MQ=255)                                                                                                                                   ataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAatcat     >  1:175468‑M2/8‑143 (MQ=255)                                                                                                                                   ataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAatcat     >  1:632345‑M2/8‑143 (MQ=255)                                                                                                                                    tAAATGTATAAGTCATACTAGTGTTTTGGGTGTATTTGAGTATGTGAAAAAGTTTTTCGCTACGATAGCAAGCAAAAATGAAAGAGGAATAATAGAAATGGGATGTTGAGCACAGTCAAAACAACACGCCGTAAAGAataatc    >  2:702233/1‑143 (MQ=255)                                                                                                                                     aattgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCt   <  2:110839‑M2/138‑1 (MQ=255)                                                                                                                                      aatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTg  <  2:279886‑M2/139‑1 (MQ=255)                                                                                                                                      aatgTATAAGTCATACTTTTGTTTTGGGCGTATGTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTg  <  2:486158‑M2/139‑1 (MQ=255)                                                                                                                                          |                                                                                                                                           GCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTG  >  NC_000913/1979142‑1979417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑