Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	56.8 / NA	21	I204S (ATC→AGC)	wcaA	putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (11/10);  total (11/10)

GATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132648‑2132925                                                                                                                                            |                                                                                                                                           gaccTGGTCGAGGTAAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTGTGGGCTTGCGGTAAAAGTGGAAGTCCCCGGAGACTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGctct                                                                                                                                         <  1:274583/140‑1 (MQ=255)   tCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCTGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                       <  2:450346/143‑1 (MQ=255)       gTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCg                                                                                                                                   >  1:616890/1‑143 (MQ=255)                       tGATATGTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAGAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTCATATGCCGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                   <  2:475308/143‑1 (MQ=255)                                    tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc                                                                                                      >  1:79061/1‑143 (MQ=255)                                    tGGCGCGGTCGAATGTGTTTTTGTGCTTGCGGTAGAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc                                                                                                      <  2:20440/143‑1 (MQ=255)                                      gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAGTc                                                                                                    >  1:207150/1‑143 (MQ=255)                                         cgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccac                                                                                                 <  2:170515/143‑1 (MQ=255)                                                          gtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCCCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCGGGAAGa                                                                                >  2:456357/1‑143 (MQ=255)                                                            gCTTGCGGTAACAGTGGTAGTACCCGGAGAATTTTTTCGGTGACGAGTTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATg                                                                              <  2:354833/143‑1 (MQ=255)                                                              tggCGGTAGAGTTGGAAGTATCCGGGGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTc                                                                            <  2:573993/141‑1 (MQ=255)                                                                  ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCAACACCCGCAGGCCGCCGTCGTaa                                                                        >  2:516507/1‑143 (MQ=255)                                                                                       agaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg                                                   <  1:45230/143‑1 (MQ=255)                                                                                                tccgTGAGGGGGTGGCCTGCCTGTCACCGCGGTTCATATGCACGCGCCGCTTCGTCTCGTATGCTTACAACCGTTCGCCGTACTCCACCACCACGCGCAGGTAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaac                                          <  2:375191/140‑1 (MQ=255)                                                                                                   gggACGGGGTGATCTTCTGGTCACTGTGATTTATATGCAGGCTCTGCGTCGCCGCTTCTACTTTCATCGGTTGTGCGTTGTGCATTATGATGCGCAGGAAGATGTCGTAATCCTGTGGCGCTTTCAGTTCGGTATCGaacaaa                                       <  2:77837/141‑1 (MQ=255)                                                                                                    tGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaac                                      >  1:643417/1‑143 (MQ=255)                                                                                                              aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAACCACTCTTTGaa                            >  1:117577/1‑143 (MQ=255)                                                                                                               tCTGCATTTCACCGTGATTAATAGGCAGGCTCTGCGCCGCCCCCTCCACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa                           <  1:695183/143‑1 (MQ=255)                                                                                                                 tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTCTCGAACAAACACTCTTTGAAACg                         >  2:622976/1‑143 (MQ=255)                                                                                                                 tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTCCGGTACCGAAAACCAACTCTTTGAAACg                         >  2:84832/1‑143 (MQ=255)                                                                                                                  gCATTTCACCGTGATTAATATGCAGGCTATGCGTCGCCTCTTCTACTTTACACGGTTAGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCATGTGCCTCTTTCACTTCGATATAAAACGAACACTATATGacaaaa                        >  2:402936/1‑138 (MQ=255)                                                                                                                                        cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTCAAACGCCATGCCCAGGTAAAGACCTGGt  >  2:201112/1‑143 (MQ=255)                                                                                                                                            |                                                                                                                                           GATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132648‑2132925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑