Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,132,7870AC100.0% 56.8 / NA 21I204S (ATC→AGC) wcaAputative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (11/10);  total (11/10)

GATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132648‑2132925
                                                                                                                                           |                                                                                                                                          
gaccTGGTCGAGGTAAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTGTGGGCTTGCGGTAAAAGTGGAAGTCCCCGGAGACTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGctct                                                                                                                                         <  1:274583/140‑1 (MQ=255)
  tCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCTGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                       <  2:450346/143‑1 (MQ=255)
      gTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCg                                                                                                                                   >  1:616890/1‑143 (MQ=255)
                      tGATATGTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAGAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTCATATGCCGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                   <  2:475308/143‑1 (MQ=255)
                                   tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc                                                                                                      >  1:79061/1‑143 (MQ=255)
                                   tGGCGCGGTCGAATGTGTTTTTGTGCTTGCGGTAGAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc                                                                                                      <  2:20440/143‑1 (MQ=255)
                                     gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAGTc                                                                                                    >  1:207150/1‑143 (MQ=255)
                                        cgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccac                                                                                                 <  2:170515/143‑1 (MQ=255)
                                                         gtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCCCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCGGGAAGa                                                                                >  2:456357/1‑143 (MQ=255)
                                                           gCTTGCGGTAACAGTGGTAGTACCCGGAGAATTTTTTCGGTGACGAGTTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATg                                                                              <  2:354833/143‑1 (MQ=255)
                                                             tggCGGTAGAGTTGGAAGTATCCGGGGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTc                                                                            <  2:573993/141‑1 (MQ=255)
                                                                 ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCAACACCCGCAGGCCGCCGTCGTaa                                                                        >  2:516507/1‑143 (MQ=255)
                                                                                      agaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg                                                   <  1:45230/143‑1 (MQ=255)
                                                                                               tccgTGAGGGGGTGGCCTGCCTGTCACCGCGGTTCATATGCACGCGCCGCTTCGTCTCGTATGCTTACAACCGTTCGCCGTACTCCACCACCACGCGCAGGTAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaac                                          <  2:375191/140‑1 (MQ=255)
                                                                                                  gggACGGGGTGATCTTCTGGTCACTGTGATTTATATGCAGGCTCTGCGTCGCCGCTTCTACTTTCATCGGTTGTGCGTTGTGCATTATGATGCGCAGGAAGATGTCGTAATCCTGTGGCGCTTTCAGTTCGGTATCGaacaaa                                       <  2:77837/141‑1 (MQ=255)
                                                                                                   tGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaac                                      >  1:643417/1‑143 (MQ=255)
                                                                                                             aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAACCACTCTTTGaa                            >  1:117577/1‑143 (MQ=255)
                                                                                                              tCTGCATTTCACCGTGATTAATAGGCAGGCTCTGCGCCGCCCCCTCCACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa                           <  1:695183/143‑1 (MQ=255)
                                                                                                                tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTCTCGAACAAACACTCTTTGAAACg                         >  2:622976/1‑143 (MQ=255)
                                                                                                                tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTCCGGTACCGAAAACCAACTCTTTGAAACg                         >  2:84832/1‑143 (MQ=255)
                                                                                                                 gCATTTCACCGTGATTAATATGCAGGCTATGCGTCGCCTCTTCTACTTTACACGGTTAGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCATGTGCCTCTTTCACTTCGATATAAAACGAACACTATATGacaaaa                        >  2:402936/1‑138 (MQ=255)
                                                                                                                                       cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTCAAACGCCATGCCCAGGTAAAGACCTGGt  >  2:201112/1‑143 (MQ=255)
                                                                                                                                           |                                                                                                                                          
GATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132648‑2132925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: