Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,196,305 C→T R395C (CGT→TGT)  icd → isocitrate dehydrogenase, e14 prophage attachment site, tellurite reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,196,3050CT97.4% 92.0 / NA 39R395C (CGT→TGT) icdisocitrate dehydrogenase, e14 prophage attachment site, tellurite reductase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (21/16);  minor base G (0/1);  total (22/17)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTT  >  NC_000913/1196170‑1196444
                                                                                                                                       |                                                                                                                                           
aaaGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTgat                                                                                                                                      >  2:902736/1‑143 (MQ=255)
        tCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGCCTGATGGATGgcg                                                                                                                              >  1:180056/1‑143 (MQ=255)
           tGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCCCTa                                                                                                                           >  1:88372/1‑143 (MQ=255)
           tGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACGTCGAGTGTCTGATGGATGGCGCTa                                                                                                                           >  1:734643/1‑143 (MQ=255)
               tctATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGACAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAAct                                                                                                                       >  1:815584/1‑143 (MQ=255)
                 tattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTCAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAActgc                                                                                                                     >  1:546044/1‑143 (MQ=255)
                 tattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAAACTATGACTTCGAGTGTCTGATGGATGGCGCTAAActgc                                                                                                                     >  1:480737/1‑143 (MQ=255)
                         tctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGt                                                                                                             >  2:809542/1‑143 (MQ=255)
                            ccGCTGAGAGGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGAAGGATGGCGCTAAACTGCTGAAATGTTCa                                                                                                          <  2:631900/143‑1 (MQ=255)
                              gCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCaga                                                                                                        >  2:513245/1‑143 (MQ=255)
                              gCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCaga                                                                                                        >  2:513115/1‑143 (MQ=255)
                                    atgatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAAACAACGCGAACACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTGCAGAGTATgg                                                                                                  >  1:310270/1‑143 (MQ=18)
                                           tgcGCCACATGGGTTGGACCGAAGCGGCTGACTTACTTGTTAACGGTCTGGAAGGCGCAATCAACGCGAAAACCATAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcgcg                                                                                           >  2:540677/1‑143 (MQ=18)
                                           tgcGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcgcg                                                                                           >  2:234554/1‑143 (MQ=255)
                                                      ggTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaaa                                                                                >  2:18985/1‑143 (MQ=255)
                                                               gAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATg                                                                       <  1:717548/143‑1 (MQ=255)
                                                                 aGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAAGCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGAGCGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGAGTATTGTTTATCGAAAACATGTAATGcc                                                                     <  1:581262/143‑1 (MQ=17)
                                                                        gACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGttt                                                              <  2:836963/143‑1 (MQ=255)
                                                                           ttAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGtt                                                           >  1:273117/1‑143 (MQ=255)
                                                                                   tataGGTATGGAAGGCGCGATCAAGGCGAAAACCGTAACCTCTGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGtgcgtttttt                                                   <  1:890635/140‑10 (MQ=18)
                                                                                   taaaataaTGGAAGGCGCAATCAATGCGCAAACCGTAACATATGACTTCGAGTGTGTGATGGATGGCGCTAAACCGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATttat                                                   <  2:346223/136‑1 (MQ=18)
                                                                                        gTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGACATGTTCAGAGTTTGGTGACGCGAGCATCGAAAACATGTAATGCCGTAGTTTGTTAACTTTATTGACg                                              >  2:280250/1‑143 (MQ=21)
                                                                                        gTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACg                                              >  2:593334/1‑143 (MQ=255)
                                                                                         tATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACgg                                             <  2:23015/143‑1 (MQ=255)
                                                                                            atcttcgccgtgtaaCGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAg                                          <  1:92801/130‑1 (MQ=25)
                                                                                                  cgcAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAAc                                    >  2:197860/1‑143 (MQ=255)
                                                                                                          aCGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCAgt                            >  1:394020/1‑143 (MQ=255)
                                                                                                             cgAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCgttgt                         <  2:573822/143‑1 (MQ=255)
                                                                                                                 aatcGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGttttt                     <  1:456867/140‑1 (MQ=255)
                                                                                                                  aCCGTAACCTAGGACTTCGAGGGTCTGATGGATGGCGCTAAACGGCTGAAATGTTCAGAGTTGGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGtttttt                    <  1:922125/143‑1 (MQ=255)
                                                                                                                  aCCGGAACCTATGATGTCGAGTGTCGGATGGATGGCGCTAAACTGCTGAAATGTTCAGCGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGtttttt                    <  1:305539/143‑1 (MQ=37)
                                                                                                                    tgtgcccgttGACCTCGAGTGTCTGATGGATGGTGCTATTCTGTTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACGGGTAATGCCGTAGTTTGTTAAGTTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGt                  <  1:147199/134‑1 (MQ=25)
                                                                                                                      tAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTa                >  1:669385/1‑143 (MQ=255)
                                                                                                                        aCCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAgg              <  2:340552/143‑1 (MQ=255)
                                                                                                                        aCCTATGACTTCGAGTGTCTGAAGGATGGCGCTAAACTGCTTGAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAgg              <  2:915436/143‑1 (MQ=255)
                                                                                                                             tGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTCTTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGgctgct         >  1:749095/1‑143 (MQ=255)
                                                                                                                                 ttCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACg     >  2:885502/1‑143 (MQ=255)
                                                                                                                                    gAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGtt  <  1:513245/143‑1 (MQ=255)
                                                                                                                                    gAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGtt  <  1:513115/143‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                           
AAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTT  >  NC_000913/1196170‑1196444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑