Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,145,087 G→C 12.1% R365P (CGC→CCC)  pflD → putative glycine radical domain‑containing pyruvate formate‑lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,145,0870GC12.1% 297.7 / 7.7 133R365P (CGC→CCC) pflDputative glycine radical domain‑containing pyruvate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base G (66/49);  new base C (2/16);  total (68/65)
Fisher's exact test for biased strand distribution p-value = 2.41e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

GGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGA  >  NC_000913/4144851‑4145324
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                              
gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACCTCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGCTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGCGTAACCGATAACCGATCTAGCGCGGTGCACC‑T‑GCTTCCGTACCTTTACCT‑TCACGCCTACCAAAGCGTGCAATTTCCGCAACCGCACCTAGGAGGgcgc                                                                                                                                                                                                                                               >  2:401063/1‑238 (MQ=255)
gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TTACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTgcgc                                                                                                                                                                                                                                               >  1:15880/1‑238 (MQ=255)
gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGACACCGAACCTCGGCGTgcgc                                                                                                                                                                                                                                               >  1:135082/1‑238 (MQ=255)
gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATAACAGCGTGCAATTAACGCAACCGAACCTAGGCGTgagc                                                                                                                                                                                                                                               >  1:106653/1‑238 (MQ=255)
gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGCAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTGTCAAAGCCTGCAATTACGGCAACCGAACCTCCCCGTgcgc                                                                                                                                                                                                                                               >  2:1205975/1‑238 (MQ=255)
gggCGAAGATGCGGCGTTCCTGAAAGAACTGCCCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGCTAACCGAGAACGGACGTAGCGCGGTGATCG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTccgc                                                                                                                                                                                                                                               >  1:10909/1‑238 (MQ=255)
 ggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGCACCTCGGCGTgcgc                                                                                                                                                                                                                                               >  2:444945/1‑237 (MQ=255)
  gCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCCCCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGCCGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGGGTGCGCACt                                                                                                                                                                                                                                            >  1:479167/1‑239 (MQ=255)
   cGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACt                                                                                                                                                                                                                                            <  1:737940/238‑1 (MQ=255)
   cGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACt                                                                                                                                                                                                                                            <  1:159532/238‑1 (MQ=255)
     gtctgatagaacggcaacatataCTGGTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑G‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCACTTACCGCAACCGAACCTCGGCGTGCGCACTAAc                                                                                                                                                                                                                                         >  1:20900/23‑239 (MQ=255)
      aGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAAc                                                                                                                                                                                                                                         >  1:962532/1‑238 (MQ=255)
           cggcgTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGGGCCATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTg                                                                                                                                                                                                                                   >  1:828852/1‑239 (MQ=255)
                ttCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCg                                                                                                                                                                                                                               >  1:544447/1‑238 (MQ=255)
                  ccTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGac                                                                                                                                                                                                                             >  1:140776/1‑238 (MQ=255)
                  ccTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGac                                                                                                                                                                                                                             >  1:255511/1‑238 (MQ=255)
                      aaaGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGc                                                                                                                                                                                                                         <  1:852206/238‑1 (MQ=255)
                               cTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTgcg                                                                                                                                                                                                                                                <  1:261478/206‑1 (MQ=255)
                               cTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCCTTCCtgat                                                                                                                                                                                                               >  1:1071362/1‑239 (MQ=255)
                                         tATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGCTCCTGATGAAAAccgcc                                                                                                                                                                                                     >  1:270037/1‑239 (MQ=255)
                                              gTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGa‑aa                                                                                                                                                                                                >  1:578290/1‑238 (MQ=255)
                                                 aaaTGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGCACCTCGGCGTGCGCCCTAACCCCTTTGTCGACACGCCGTTCCTGATGAAAAACGCCGA‑AAccct                                                                                                                                                                                            >  2:386638/1‑237 (MQ=255)
                                                 aaaTGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCAt                                                                                                                                                                                            >  1:4159/1‑239 (MQ=255)
                                                          gtcagtgggcttgtGCGCACCCCCAGGAGCGCGGTTTATTTCGCCGGTTCCCCGA‑CCGGCTATTCCCCACTGCTTGGCTGGTTACCCGAGAACGGACGTAGCGTGGTGAACG‑T‑GCTTCCGTTCCTTTCCCT‑TGACGCCTTTCAATGCGTCCACTTCCCGCACCCGAACCTCGCGGTGCCCACTACCTCTTTGATCGACCCGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCgg                                                                                                                                                                                   <  2:206547/226‑1 (MQ=255)
                                                             aTCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATCACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACCCGCCGATCCTGATGCAAACCGCCGA‑AACCATTCGCTTCGGTa                                                                                                                                                                                 >  1:219539/1‑238 (MQ=255)
                                                               cGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCATGTTGAAAACCGCCGA‑CACCATTCGCTTCGGTACCg                                                                                                                                                                              >  1:1194119/1‑239 (MQ=255)
                                                               cGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGCTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGGGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTACCTTCGGTACCg                                                                                                                                                                              >  1:923594/1‑239 (MQ=255)
                                                                              caccaGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCt                                                                                                                                                               >  2:288355/1‑239 (MQ=255)
                                                                                ccaGTGGCGCGTGTTTTTTC‑CCGGTTTCCCCACCCGGCTCTCCCGCCCCGCTCGGCGGTTTACCCCAGACCGGACGTAGCGCGGTGAACG‑T‑GCTTGCGTTCCTTTGCCT‑GGACCCCTACAAAAGCTTGCATTTCCCGCAACCGACCCTCGGCGGGCCCACAACCGCTTGTTTCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCttt                                                                                                                                                             <  2:1071362/239‑1 (MQ=255)
                                                                                   gtgccgggctgtattcggCCGGTTGCGCAC‑CCGGTGATCCCGCAGTGCGCGGCGGTGTAACGGAAAACGGAGGTGGCGCGGTGACCT‑G‑TCTTCCGTTCCTTTCCGT‑TGCCGCTTAGCAAGCCGTTCAATTCCCCCAACCGAACCTCGCCGGGCGCAAAAACGCCTTTCCCGACACGCCTGTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTaa                                                                                                                                                           <  2:165096/221‑1 (MQ=255)
                                                                                     aGCGCGCGTTATTCCGCCGGTTTCCCGA‑CCGGCTATCCCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCACCCGACCCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGa                                                                                                                                                        <  1:196885/239‑1 (MQ=255)
                                                                                        gcgcgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACCCGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGAttg                                                                                                                                                     >  1:267655/1‑237 (MQ=255)
                                                                                        gcgcgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGATCCTGATGAAAACCGCCGG‑AACCATTCGCGTCGGTACCGGCATTCCGCAAATCTTTAACGCTGa                                                                                                                                                     >  1:769894/1‑239 (MQ=255)
                                                                                        gcgcgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAAAGCCTTGATCGACACGCCGTTCCTGATGAAAAACCCCGA‑AACCATTCGCTTCGGGACCGGGATTACGCCAATCTTTAACGGTGa                                                                                                                                                     >  2:265141/1‑239 (MQ=255)
                                                                                        gcgcgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGGGCAATTACCGCAACCGAACCTCGGCGTGCGCCCTAACGCCCTTATCGACACCGCGTTCCTAATCAAAACCGCCCACAACCA‑TCGCCTCGGGCCCGGGATTCCGCAAATCTTTAACgggag                                                                                                                                                     >  2:134176/1‑235 (MQ=255)
                                                                                        gcgcgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTACAAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCAATACCGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATg                                                                                                                                                      <  2:356071/238‑1 (MQ=255)
                                                                                        acgcgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCCTGGAATTACCGCCACCGAAACCCGACAGGCGCACTAACGCCCTGATCGACACGCCGTTCCCGATGAAACCCCCCGC‑AACCATTCACTTCTGTCCCGGCATTCCCCCCATCTTTAACGAtca                                                                                                                                                     >  2:171263/2‑237 (MQ=255)
                                                                                           cgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCGTGATCGACCAGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAgt                                                                                                                                                  >  1:197373/1‑239 (MQ=255)
                                                                                              tATTTCCCGGGTTCCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTACCCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTCCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGAAATTACCGCAACCGAACCCCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAgtggt                                                                                                                                               <  2:1094681/239‑1 (MQ=255)
                                                                                                     ccGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGGGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGC‑AACCATTCGCCTCGGTACCGGTATTCCGCAAATATTTAACGATGAAgtg                                                                                                                                                 >  1:618728/1‑230 (MQ=255)
                                                                                                         ttccctgA‑CGGGCTATCGCTCACTGCTCGGCGGCTCACCTGAGACCGGACGTAGGCGGGTAAAGG‑G‑GCTTTCGTCCCTTTCCCT‑AGACGCCTATCAAACGGGGAAAGCCCCGCAACCGACCCGCGCCGGGCGACCGACCGCCTTGATGGACACGCGGTTCCTGAGAAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCg                                                                                                                                     <  2:1203600/232‑1 (MQ=255)
                                                                                                                a‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCa                                                                                                                              >  1:185189/1‑238 (MQ=255)
                                                                                                                    ggCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGAGACGTTAAAT‑T‑GCTTTGGTTCCTTTGCCT‑TGACGCCTATCAAAGAGTGCAATTACCGCAACCGAACCTCGGCGGGCGCACTAACGCCATTATCGAACCGCCCTTCCTGATGAAAACCGGCGA‑AACCCATCGCTTCGGCACCGGTAATCCGTAAATCTTTAAAGATGAAGCGGCGGTGCCAGCCTTCCTCaaac                                                                                                                           >  2:1028997/1‑236 (MQ=255)
                                                                                                                           ccGCCCTGCCCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTGTGGTTCCTTTGCCT‑TGACGCCTATAAAAGCGTGCAATTCCCGCAACCGAACCTCGGCGTGCGCCCTAACGCCTTGATCGCCACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGtt                                                                                                                   <  2:874974/239‑1 (MQ=255)
                                                                                                                           ccGCACCGCCCGGCGGGTTAGCCGAGAACGGCCGAGGCGCGGTGACCG‑T‑GCTCGCGTACGTCTTCTC‑TGGCGCCCATCACGGCGTACATTTGCCGCACCCGTACTCCGCGGCGCGCCCAACCGCATGCATCAAGACCCGGTTCCCGAAGAAAGCCGCCGA‑AACCATTCGCGTCGGTACCGGTATTCCGCAAATCTTTAACGATGAGGTGGTGGTGGCAGCGTTCCTCACCCGTGGCGtt                                                                                                                   <  2:541071/239‑1 (MQ=255)
                                                                                                                            cGCACTGGTCGGGGGGTTAACCGAGAACGGCCGTCGGGGGGTGAAGT‑T‑GCTTTCGTCCCTTTGCTT‑GGCCGCCTATAAAAGCGTGAAATTCCCGCACCCGACCCTGGGCGTGCCCACAACCGCTTGGATCGACACGCCGTTCCTAATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGttt                                                                                                                  <  2:849167/239‑1 (MQ=255)
                                                                                                                              cACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCtt                                                                                                                                                              >  2:284880/1‑193 (MQ=255)
                                                                                                                              cACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATAAAAGCGTGAAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCtt                                                                                                                                                              <  1:284880/193‑1 (MQ=255)
                                                                                                                               aCTGCTCGGCGGGTTAACCGAGAAAGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCTAACCTCGGCGGGCGCACTAACGCCTTGATCGACACGCCGTTCCTTATGAAAACCGCCGG‑AACCATTCCCTTCGGTACCGGGATTCCGCAAATATTTAACGATGACGTGGTGGTGGCACCGTTCCTCTACCGTGTCTTTTc                                                                                                                 >  2:546463/1‑237 (MQ=255)
                                                                                                                                   cTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTTCCT‑TGACGCCTATCAAAGCGGGCAATTACCGCAAACGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCCTTCGCTTCGGTACCGGTATTCCGCCAATCTTTAACCATGACATGGCGGTGCCACCGTTCCTCCACCCTGGCGTTTCGCttgc                                                                                                           >  2:487017/1‑236 (MQ=255)
                                                                                                                                   cTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGGAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGGTGTGCCAGCGTTCCCCAACCGTGGCGTTTCGCTGGa                                                                                                           >  2:506482/1‑239 (MQ=255)
                                                                                                                                   cTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGa                                                                                                           >  1:75009/1‑239 (MQ=255)
                                                                                                                                   cTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTAACGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGa                                                                                                           >  1:1004555/1‑239 (MQ=255)
                                                                                                                                   cTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTACCAAAGCGTGCAATTACCGCAACCGAACCTCTGCGTGCGCACTAACGCCTTGCGCGACCCGCCGTTCCTGATGAAACCCGCCGG‑AACCATTCGCTTCGGTACCGCTAGTCCGCAAAACTTTAACGATGACGTGGTGGCGACAGCGGTCGCCAACCGGGGCGTTTCGcgtgg                                                                                                           >  2:227456/1‑235 (MQ=255)
                                                                                                                                        cggGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTc                                                                                                                                                                                                                    <  2:789146/130‑1 (MQ=255)
                                                                                                                                        cggGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTc                                                                                                                                                                                                                    >  1:789146/1‑130 (MQ=255)
                                                                                                                                           gTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATgcgc                                                                                                   >  2:297736/1‑239 (MQ=255)
                                                                                                                                             tatgcGAGAGCGGACGTAGCAGGGTGAACG‑T‑GCTTTCGTTCCTGTTCCG‑TGACGCCTCCAAAAGCGTGCAATTACGCCACCCGAACCTCGGCTCGCCCACTACCGCCTTGATCGACCCGCCGTTCCTGAATAAACCCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATgcgcgc                                                                                                 <  2:147355/235‑1 (MQ=255)
                                                                                                                                               ccaggaaaCGGATGTGGGGCGGGGAACT‑G‑CCTTTCGTTCGTTTGCCT‑GGACGCCTAACAAAGCGTCCAATTCCCGCAACCGAACCCGGGCGGGCGCAAAAACGCTTTGGTGGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGa                                                                                               <  2:313685/233‑1 (MQ=255)
                                                                                                                                               aCCGAGCACGGACGTGGCGCGGTGACCG‑G‑ACTTATGTTCCTTTGCCT‑GGACGCATATCAATGCGAGCAATTCCCGAAACGGAACCTTGGCGGGCGCACTAACGCCTTGACCGCAACGCCTTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGTCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGa                                                                                               <  2:940035/239‑1 (MQ=255)
                                                                                                                                                ccgagagatggaggtgcggGGTGGACG‑TCGCTTTGTTTCTTGTGCCT‑GGCGCCCTATCAATCGTTGCAATTACGGCACCCAACCCTGCGCGTGCGCACTAACGCTTTTATCGAGACGCGGTTCCTGATAAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGa                                                                                               <  2:34717/224‑1 (MQ=255)
                                                                                                                                                    aaagggcggggggggggTGAACT‑G‑CCTTCCGTCCTTTTGCCT‑TGCCGCCTATCAAGGCTAGCAATTACCGCACCCGACCCCCGGCGGGCGCATAAACGCCTTGATGGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTAtt                                                                                          <  2:6228/224‑1 (MQ=255)
                                                                                                                                                      ccGGCCGGAGCGCGGTGACCC‑T‑GCTTCCGTTCCTTTGCTT‑GTCCCCCTCTAAAGGCGTGCAATTACCGCACCCGAACCCGGGCGTGCCCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTcc                                                                                        <  2:941782/238‑1 (MQ=255)
                                                                                                                                                      aCGGCCGTGGCGGGGT‑AACGTT‑GCTTTCGTTCCTTCGCGT‑TGACCCTTACAAAAGGGTGCAATTCCCGCAACCGACCCTGGGCTTGCCCCCAAACGCCTGGACCGCAACGCCGTTCCTGATAAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTAtt                                                                                          <  2:257950/237‑1 (MQ=255)
                                                                                                                                                      aCGGACGTACCGCGGTAAACT‑T‑GCTTTCGTTCATTTCCCT‑TGCCGCAAATCAAAGCGTGCACTTCCCGCACCCGACCCTGGGCGGGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTAtt                                                                                          <  2:962532/237‑1 (MQ=255)
                                                                                                                                                         gACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGCTGCGCGCGACTATTCCGTa                                                                                     >  1:995037/1‑239 (MQ=255)
                                                                                                                                                          aCGTCGCGGGGTGAACG‑T‑GCTTTCGTTCCTTGCCCT‑TGACCCCTATCAAGGCGTGCAATTGCCGCAACCGAACCCGGGCGTGCGCACTAACGCTTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTa                                                                                     <  1:299791/238‑1 (MQ=255)
                                                                                                                                                          aCGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGACCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTa                                                                                     <  1:230425/238‑1 (MQ=255)
                                                                                                                                                              cgcgcGTTGAACG‑T‑GTTTTCGTTCCTTTCCCT‑TGACGCCTACAAAACCTTGCAATTCCCGCACCCGACCCCCGGCGGGCGCACTACCGCCTTGTTCGACACGCCGTTCCTGAGGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTggg                                                                                <  1:1093041/238‑1 (MQ=255)
                                                                                                                                                              accgcgTTGAACG‑T‑TCTTTCGTTCCTTTGCCT‑GGACGCCTACCAAAGCGTCCAATTACCGCACCCGACCCCCGGCGTGCGCACTACCGCTTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTggg                                                                                <  1:660126/237‑1 (MQ=255)
                                                                                                                                                              aGCGCGGTGACCG‑T‑GCTTTCTTTCCTTGGCCT‑TGCCGCCTATCAAAGCGTGCAATTCCCGCAACCGACCCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTggg                                                                                <  1:151214/239‑1 (MQ=255)
                                                                                                                                                              aGCGCGGGGAACT‑T‑GCTTTCGTCCCTTTGCCT‑GGACGCCATACAAGGCGTGCAATTCCCGCACCCGAACCGCGCCGTGCCCACTACCGCCTTGATCGCCACGCCGTTCCTGATAAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTggg                                                                                <  2:995037/239‑1 (MQ=255)
                                                                                                                                                               gcgggttGAACG‑T‑GCTTTCTTCCCTTTGCCT‑TGACCCCTACCAAAGCGTGCATTTCCCGCACCCGACCCCGGGCGTGCGCACTAACCCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTggg                                                                                <  1:284201/232‑1 (MQ=255)
                                                                                                                                                                   ggTGAACG‑T‑GCTTTCGTTCTTTTGCCT‑TGCCGCCTATCAACGCGTGCAATTCCCACACCCGACCCCCGGCGTGCCCACTAACCCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCtgtg                                                                           <  1:714027/239‑1 (MQ=255)
                                                                                                                                                                    gTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGAGACTATTCCGTAGTGGGCtgtgt                                                                          >  1:595365/1‑239 (MQ=255)
                                                                                                                                                                    gTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGGGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCtgtgt                                                                          >  1:332115/1‑239 (MQ=255)
                                                                                                                                                                    gTGAACG‑T‑CTTTTCG‑TCCTTTTCCTGTGACCCCTACCAAGCGGTGAAATTCCCGCACCCGAGCCTGGGCGTGCCCACTACCGCTTTGATCGACACGCCGTTCCTGATAAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCtgtg                                                                           <  2:76244/238‑1 (MQ=255)
                                                                                                                                                                               ctttgcttCTTTCGCCT‑TGGCGCCTATCAAAGCGGTCAATACCCGCACCCGACCCTCGGCGTGCGCTCTACCGCGTTGATCGACACGCCGTTCCGGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTAt                                                                  <  2:281918/232‑1 (MQ=255)
                                                                                                                                                                                tttCGTTCCTTTGCCT‑TGACGCCTACCAAGGCGGGCAATTCCCGAAACCGACCCTCGGCGTCCGCCTTACCGCTTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATGCGCTGCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCt                                                                <  1:171263/239‑1 (MQ=255)
                                                                                                                                                                                   cGTTCTTTGGCCT‑TGACCCCTAACAAAGCGGGCATTTACCGCAACCGACCCTCGGCGTGCGCACTACCGCTTTGTGCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTAtt                                                             <  2:1118699/239‑1 (MQ=255)
                                                                                                                                                                                      tcttttGCCT‑TGACGCCTACCAAAGCGTGCAATTCCCGAAACCGACCCTCGGCGTCCGCCCTAACGCTTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTAGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTcc                                                           <  1:244806/235‑1 (MQ=255)
                                                                                                                                                                                          tggCCT‑TGACGCCATCCAAAGCGCGCATTTCCCGCACCCGAACCGCGGCGTGCCCACTAACGCCTTGAGCGACACCCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCa                                                      <  2:661218/237‑1 (MQ=255)
                                                                                                                                                                                                    cgccatcacaggggTGCAATTCCCGCACCCGACCCTCGGCTCGCGCACAACCGCCTGTATCGACCCGCCTTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACg                                             <  2:578290/226‑1 (MQ=255)
                                                                                                                                                                                                    cGCTTACCAAACCGTGCAATTCCCGAACCCGACCCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACg                                             <  1:388270/239‑1 (MQ=255)
                                                                                                                                                                                                    cGCCTATCAAAGCGTGCAATTCCCGCAACCGACCCTCGGCTTGCCCCCTAACGCCTGGATCGCCACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACg                                             <  1:1009671/239‑1 (MQ=255)
                                                                                                                                                                                                           cAAAGCGTGAATTTCCCGCACCCAACCCCCGGCGTGCGCTCTAACGCTTTGATCGACACGCCTTTCCTAATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCa                                      <  1:920573/239‑1 (MQ=255)
                                                                                                                                                                                                             aaGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTg                                        >  2:21199/1‑235 (MQ=255)
                                                                                                                                                                                                                  tGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATc                               >  1:397366/1‑239 (MQ=255)
                                                                                                                                                                                                                  tGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATc                               >  1:1010628/1‑239 (MQ=255)
                                                                                                                                                                                                                  tGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGGGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGGATGACATc                               >  1:965147/1‑239 (MQ=255)
                                                                                                                                                                                                                   gCAATACCCGCACCCGACCCTGGGCGTCGGCACTAACGCCTGGACCGCCACGCCGTTCCTGATGAAAACCGCCGA‑AACCATGCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATcg                              <  1:676418/239‑1 (MQ=255)
                                                                                                                                                                                                                    ctactACCGCCACCACACCTCGTCGTCCCCACGAACGCTTTGAGCGCATCGCCGTCCCTGAAGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTGTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGGGTTTCGCTGTAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGACACTACGGCTTGCATGACATcgc                             <  2:267655/235‑1 (MQ=255)
                                                                                                                                                                                                                    cAATCACCGCAACCGAACCTCGGCGGGCGCACTAACGCCTGGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCGTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATcgc                             <  1:1128298/239‑1 (MQ=255)
                                                                                                                                                                                                                             cccccGCACCCCGGCGTGCCCCCAAACCCTTTGACCGACACGCCGTCCCAGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTaa                    <  2:140776/236‑1 (MQ=255)
                                                                                                                                                                                                                             cAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGCATATTCCGTAGTGGGCTGTGTGGAATTATCTATTCACGCCAGAACCTACGGCTTGCATGACATACGGATTTTTaa                    >  2:861683/1‑239 (MQ=255)
                                                                                                                                                                                                                               aCCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGc                                           >  2:1055532/1‑214 (MQ=255)
                                                                                                                                                                                                                                   aaCCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGGGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCCTGACATCGCGATGTTTAATct                 >  1:268723/1‑236 (MQ=255)
                                                                                                                                                                                                                                     ccTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGGGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGa            >  2:814671/1‑239 (MQ=255)
                                                                                                                                                                                                                                            gTGCGCCCCAACGCTGTGACCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGAt     <  2:818582/239‑1 (MQ=255)
                                                                                                                                                                                                                                               cgcACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTTGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCCATGTTTAATCTGCTGAAAGTGATGGa  >  1:984047/1‑239 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                              
GGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCT‑TGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGA  >  NC_000913/4144851‑4145324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑