Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,151,320 T→G 20.7% H593P (CAT→CCT)  ppc ← phosphoenolpyruvate carboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,151,3200TG20.7% 87.0 / 2.0 48H593P (CAT→CCT) ppcphosphoenolpyruvate carboxylase
Reads supporting (aligned to +/- strand):  ref base T (26/8);  new base G (3/8);  total (32/16)
Fisher's exact test for biased strand distribution p-value = 8.52e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAGCCGCGGTAGACATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGT  >  NC_000913/4151085‑4151554
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           
gtaGCCGCGGTAGACAGCGCAGGAGATGACTGACAGTTCCAGCATAATGCGTCGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCGCCGGTATAAAGCGACAGGCTGCTGACGGGGATTTCTGGCAGACCGTATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGACGCCTTTCAGGCTTCCTGGGGGTTGTGACAGCAGCG‑‑CCGCATGAg                                                                                                                                                                                                                                          <  2:957535/239‑1 (MQ=255)
 taGCCGCGGTAGACATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGGCTTTCAGGCTTCCTGGCGGTTGTGACAACAGCG‑‑CCGCATGGGc                                                                                                                                                                                                                                         >  1:1107575/1‑239 (MQ=255)
  aGCCGCGGTAGACATCGCAGGTGATGACTGACAGTTCATCCATAATGCTACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGc                                                                                                                                                                                                                                         <  2:592664/238‑1 (MQ=255)
                tCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCcgcg                                                                                                                                                                                                                          <  1:725684/239‑1 (MQ=255)
                           gactgacAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCCGCG‑‑CCCCAGGAGCAGGTGCGCCCCCGCGACCAAAGGac                                                                                                                                                                                                                >  2:1130025/1‑237 (MQ=255)
                           gactgacAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCCTGAGCAGGTGCGCCGCCGCGACCCATGGaa                                                                                                                                                                                                                >  1:421465/1‑238 (MQ=255)
                                                                 tCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGCTTTGACAGCACCG‑‑CCGCCGGAGCCGTTGTGCCCCCGCGACCCATGCAACCGCCGCGACCGTGGGATAACggccagtccacaccccca                                                                                                                                                                         >  2:149569/1‑222 (MQ=255)
                                                                 tCGGCTCCGGCGGGGGCAGCAGGTTGGCTTCCAGACTCGCCCCCGTATAAAGCGACAGGCGGCTGACGGTGATTTCTGGCAGACCCTATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTAAGGCTTCCTGGCGGTTGTTAGCGCACCG‑‑CCCCACGTGCCGGTGCGCCCCCCCGACCCATGGACACGCCCGGCGCGTGGGACGACGtgcccccccaaaccc                                                                                                                                                                           >  2:607826/1‑223 (MQ=255)
                                                                        cgggggGGGCAGCAGGTTGGCTGCCAGAACCGCCGCGGTAAAAAGCGACAGGTTGCTGGCGGTGATTTCTGCCAACCAATTTTAAAAGGGGTCCATCTCGCCTTGTCCGTTCGCGCGCGGGCCTCCTGTCGGGCTCCTGGGCGGTGGTGACGGCGGCG‑‑CCGCAGGAGCAGGGGCCCCGCCGCGCCAAAGGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGc                                                                                                                                                                  <  2:193737/239‑1 (MQ=255)
                                                                                                       ggcccGGTATAAAGCGACAGGCTGCTGACCGGGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCAGCCTTTCAGGCTTCCTGGCGGTTGTGATAGAAGCC‑‑CCGCCCGAGCACGCGTGCCGCCGCGACCAATGGAACCGGCGCGACCCTCGAACAACCTCAGCTCACACCCCCCGTTTTCCCAGGTTTTCGTTAATGCACACGGTGACTGaca                                                                                                                                   >  2:796990/3‑237 (MQ=255)
                                                                                                         cccggtagaagggcgcgggagggtgAGGGTGATTTGTCGTAAACCATATGAAAAGCGGTTAGCCCCGCCCTGTTGGTGTGGGCGCGGGCCGCTTTCGGGCGTTCGGGGCGGTGGGGAGAGCGGCG‑‑CCGCCGGGGCGGGGGCGCCGCCGCGACCAGTGGATCCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATAtt                                                                                                                                 <  2:118501/216‑1 (MQ=255)
                                                                                                                   aGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGCGCGCCCCCGCGACCAATGGAACCGCCGCGAACGGGGGACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCCACCTGAGCCTGATATTGCGCCCCGGa                                                                                                                       >  1:324288/1‑239 (MQ=255)
                                                                                                                       aCAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCCATGGAACCGCCGCGACCGCGGCACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGaa                                                                                                                      >  1:535407/1‑236 (MQ=255)
                                                                                                                       aCAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGGGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCCCGGCCGTGGAACAACGTCAGCGCAATACCCGCTTTTTCGCCGGGTTTGCTTAATGCATCCTGGGCCCGGTACTGCGCCCAAGaa                                                                                                                      >  2:1119901/1‑236 (MQ=255)
                                                                                                                        cAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCCTGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCCGTGCCTGATATTGCGCCCCGGAAGCt                                                                                                                   >  1:319136/1‑238 (MQ=255)
                                                                                                                        cAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCCGGAAGCt                                                                                                                   >  1:1057695/1‑238 (MQ=255)
                                                                                                                        cAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGCTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCCCGACCCTGGAACAACGTCAGCCCAATACCCGCTTTTTCGCCGGGTTTGGTTAATGACACCTGTGCCTGATATTTCGCCCAGGAAGCt                                                                                                                   >  2:485535/1‑238 (MQ=255)
                                                                                                                        cAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCCGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGGTGTGACAGCAGCG‑‑GCCCAAGACCAGGTGAGCCCCCCCGACCAATGGAACCGCCGCGACCGCGGCACAACCCGAGCCCACCACCCCCTTTTTCGCCGGTTTTGTTTCGTGCATCCTGCGCCTGACATTCCGCCCCGGCAGCt                                                                                                                   >  2:569385/1‑238 (MQ=255)
                                                                                                                                     gTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCg                                                                                                     >  1:1051492/1‑238 (MQ=255)
                                                                                                                                                              aGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCCCGACCCATTGAACCGCCGCGACCGTGGGACAACGTCAGCTCCATACCCGCTTTTTCGCAGGCTTTGCTTAATGCATCCTGTGCCTGATCTTGCGCCCCGGACGCTCCCATCACCCCCCCCTCTTTTGCTTCTTCGGAACAgtcc                                                                           >  2:1156121/1‑236 (MQ=255)
                                                                                                                                                               gCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTTGCGGGTGTGATAGCAGCG‑‑CCGCATGGGCAGGTGCGCCCCCGCGGCCCATTGGACCGCCGCGAACGCGGAACCACGTCAGCTCCATACCCGGCTTTTTGCAGGGTTTGATTAATTAATCCTGTGCCTGCTATTGCGCCCAGGACGATGCCCTTCACCCCCCATCTTTTTCTGCGTCGGACTAGCCaa                                                                          >  2:829247/1‑239 (MQ=255)
                                                                                                                                                               gCGGATCATCTCGCCCTGTTCGGTTAGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCCGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGGAGCTGCCCTCACTCCCCCCTCTTTTGCTGAGTCGGACTTGCCaa                                                                          >  2:169911/1‑238 (MQ=255)
                                                                                                                                                                   atcatcTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGAt                                                                                                                                                        >  1:703679/1‑158 (MQ=255)
                                                                                                                                                                     ccccgccgccTGTTCCGGCTACGCGCGGGCCCCTGGTCGGGTCTCCGGGCGTTTGAGACCGCACCG‑‑CGGCATGGGCGGGTGCGCCGCCGCAACCAATGGAGCCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAatcat                                                                      <  2:319136/230‑1 (MQ=255)
                                                                                                                                                                                gTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCCGGTGCGCCGCCCCGACCAATGGAACCGCCGCGACCCTGGAACAACGCCAGCTCAATACCCGCTGTTTCGCAGGTGTTGATTAATGGATCCTGTGCCTCAAATTTCCCCCAGGAAGCTTGCATCACTCCCGCATCTTTTGCTTCGTCTGAATCACCAATCAACACCATCTCttt                                                          >  2:1167422/1‑238 (MQ=255)
                                                                                                                                                                                 ttCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCCGGTGCGCCGCCGCGACCAATGGAACCCCCGCGCCCCTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGGTTTGATTAATGCACCCTGTGCCTGATATTGCGCCCAGGGAGCTGCCATCACTCCCCCATCTTTTTCTGCGTCGGGATAGCCAATCATCCCCCTCCGtttt                                                         >  2:7353/1‑237 (MQ=255)
                                                                                                                                                                                          gcgcgcggCCGCCTTTCTGGTCTCCCGGGCGTTAGTGAAGGCAGCG‑‑CCGCATGAGCGGGTGCGCCCCCGCCACCAGTGAAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAAt                                                 <  1:941807/233‑1 (MQ=255)
                                                                                                                                                                                          cgcgCAGGCCGCCTGTCAGGCTTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGCGCAGGTGCGCCGCCCCGACCAATGGAACCGCCGCGGCCGTGGAACAACGTCCGCTCAATACCCGCGTTTTCCCAGGTTTTGATTAATGCATCCTGTGCCCGAGATTGCGCCCAAGAAACTGCCCTCACTCCCCACTCTTTGTCTTCCTCCGGATAGCCAAATATCTCCAACCGCTTGCCCtcactc                                                >  2:1104649/1‑234 (MQ=255)
                                                                                                                                                                                            cgcAGGCCGCCTTTCAGGCTTCCTGGCGGTTTTGAAAGAAGCG‑‑CCGCCTGAGCAGGTGCGCCGCCTCGACCAATGGGACCGCCGCGCCCGTGGAACAAAGTCAGCTCAATACCCGCTTTTTCGCAGGCTTTGATTAATGCAGCCTGTGCCTGAAATTGCGCCCACGAAGCTGCCATTTCTCCCGCATCTTTTGCTGGGGAGGAATAGCCAATCATCACCATCTCTTTGCCCTGACTcg                                               >  2:348729/1‑237 (MQ=255)
                                                                                                                                                                                            cgcAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTTCTGAGTCGGCATAGCCAATCATCACCATCTGTTTGCCCTGAATCAg                                              >  1:783427/1‑239 (MQ=255)
                                                                                                                                                                                            cgcAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAg                                              >  1:960460/1‑239 (MQ=255)
                                                                                                                                                                                            cgcAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCCGGACCAATGGAAAAGCCGCGACCGTGGAAAAACGTCCGCTCAATACCCGCTTTTTAGCAGGGTTTGATTAATGCATCCTGTGCCTGATATTTCGCCCCGGAAGCTGCCATCACTCCCGCATCTCTTGCTTGGCAGGGATAGCCAATCATCACCACCTGTTTGCCCTTCATCAg                                              >  2:265052/1‑239 (MQ=255)
                                                                                                                                                                                            cgcAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAACG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAg                                              >  1:101615/1‑239 (MQ=255)
                                                                                                                                                                                              cAGGCCGCCTTCGCGCTTCCCAGCGGTTTGGCACCCGCGCCCCGCGTGAGAAGGGCCCCCCCCGCAACCAGTGGAGCCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGc                                            <  1:1155217/239‑1 (MQ=255)
                                                                                                                                                                                                 gccgccTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCCGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCCATACCCGCTTTTTCGCAGGTTTTGATTAATGCAACCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCCTTTTCTGAGGCGGGATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCAc                                         >  2:454965/1‑239 (MQ=255)
                                                                                                                                                                                                     ccTTTCGGGCTTCCTGGGGGTTGTGACAGCAGCG‑‑CCGCAGGAGGAGGTGCGCCGCCGCCACAAAGGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATa                                     <  1:1075312/239‑1 (MQ=255)
                                                                                                                                                                                                     ccTTTCAGCCTCCCTGGCGTTGGTGACAGCAGCG‑‑CCGCGGGACGAGGTCCCCCCCCGACACCAATGAAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATa                                     <  1:1119901/239‑1 (MQ=255)
                                                                                                                                                                                                     ccTTCCAGGTTTCCGGGCGGTTGTGCAAGCGGCG‑‑CCGAATGCGCAGGGGCGCCGCCGCGACAAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATa                                     <  1:862490/239‑1 (MQ=255)
                                                                                                                                                                                                          cAGGCTTCGTGGCGGTTGTGACCGCGGCG‑‑CCGCAGGAGCAGGTGCGCCGCCGCCACAAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGt                                <  1:1096927/239‑1 (MQ=255)
                                                                                                                                                                                                            cagcttgcgtGGCGGTTGGGACAGCAGCC‑‑CCGGAGGAGGAGGTGCCCCGCCCCACCCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGt                                <  1:380792/230‑1 (MQ=255)
                                                                                                                                                                                                                  tCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAatat                          >  1:447767/1‑239 (MQ=255)
                                                                                                                                                                                                                  tCCTGGCGGTTGGGACAGCAGCC‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCCCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCCGATATTGCGCCCAGGAAGCTGCCATCCATCCCGCCTCTTTTGCTGCGTCGGCATAGCCCATCATCACCATCTGTTTGCCCTGAATCCGGCCACGATACCAGTCAaaat                          >  2:821099/1‑239 (MQ=255)
                                                                                                                                                                                                                       gtggTTGTGACAGCGGCG‑‑CGCCAGGAGCAGGCGCGCCGCCGCGACCAAGGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGag                      <  1:130362/236‑1 (MQ=255)
                                                                                                                                                                                                                       gggtttGTGACCGCGGCC‑‑CCGCTTGGGCAGGGGCGCCGCCGCGACCATGGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGag                      <  1:665682/234‑1 (MQ=255)
                                                                                                                                                                                                                                    cagcG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCCTCTTTTGCTGAGTCGGAATAGCCAATCATCACCCTCTGTTTGCCCTGAATCAGGCCCCGATACCAGTCAATATTGAGCAGCTGGGTCATg         >  2:695683/1‑238 (MQ=255)
                                                                                                                                                                                                                                       cg‑‑ccgcAGGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGTTTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCAATCCCGCATCTTTTGCTGACTCGGAATAGCCAATCATCACCATCCTTTTGCCCTGAGTCAGGCCACGAAACCCGTCAATATTGAGAAGCTGGGTCATcaca      >  2:766419/1‑238 (MQ=255)
                                                                                                                                                                                                                                          cccgcAGGAGCAGGTCCGCCCCCACCCCAGTTGTATCCGCCGCGACCGTGGAACAACGTCCGCGCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCCTCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGAc       <  2:462281/235‑1 (MQ=255)
                                                                                                                                                                                                                                            cgcATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGt  >  1:503218/1‑239 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           
GTAGCCGCGGTAGACATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGT  >  NC_000913/4151085‑4151554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: