BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	630,153	T→G	16.5%	V87G (GTG→GGG)	cstA →	carbon starvation protein involved in peptide utilization, APC peptide transporter family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	630,153	0	T	G	16.5%	239.2 / 3.6	100	V87G (GTG→GGG)	cstA	carbon starvation protein involved in peptide utilization, APC peptide transporter family protein
Reads supporting (aligned to +/- strand): ref base T (43/35); new base G (17/3); total (62/38)
Fisher's exact test for biased strand distribution p-value = 1.95e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

TCGTCTGGACAGTGCTCTCTGTAATGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGC  >  NC_000913/629916‑630390

tCGTCTGGACAGTGCTCTCTGTAATGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTg                                                                                                                                                                                                                                                      <  1:169115/239‑1 (MQ=255)

 cGTCTGGACAGTGCTCTCTGTAATGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGCATGACGCCAGCGGTGCGCCATAACGCCGGGCTGGACTAT‑GTGCCGCCGGACAAGAAAGGGc                                                                                                                                                                                                                                                     >  1:340854/1‑239 (MQ=255)

            tGCTCTCTGTAATGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGGCGCGTATGACGCCAGCGGTGCGCCATAACGCCGGGCTGGACTCT‑GGGCCGACGGGCAAGACAGTGCTGCTCGGTCa                                                                                                                                                                                                                                           >  2:1206989/1‑238 (MQ=255)

            tGCTCTCTGTAATGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGGCAAGAAAGTGCTGTTCGGTCa                                                                                                                                                                                                                                           >  1:1174184/1‑238 (MQ=255)

            tGCTCTCTGTAATGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGCAAAGAAAGGGCTGTTCGGTCa                                                                                                                                                                                                                                           >  1:96096/1‑238 (MQ=255)

              ctctctGTAATGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACTGGCTGGACTAT‑GGGCCGACGGACAAGAAAGGGCTGTTCGGGCA‑CCa                                                                                                                                                                                                                                       >  1:1092750/1‑239 (MQ=255)

              ctctctGTAATGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCa                                                                                                                                                                                                                                       >  1:1160199/1‑239 (MQ=255)

              ctctctGTAATGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGCTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACCCGTATGACGCCCGCGGTGCGCCCTAACGACCGGCTGGGCTAT‑GTTGCGACGGAAAAGGAAGGGCTAGTCGGACA‑CCa                                                                                                                                                                                                                                       >  1:717538/1‑239 (MQ=255)

                 tctGTAATGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCCGCGGGGCGCCATAACGACGGGCTGGACTAT‑GTGCCGGCGGACAAGAAAGGGCTGTTCGGTCC‑CCAttt                                                                                                                                                                                                                                    >  1:1138208/1‑239 (MQ=255)

                   tGTAATGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTg                                                                                                                                                                                                                                  <  1:914684/239‑1 (MQ=255)

                        tGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGCCCGGTATGACGCCCGCGGTGAGCCAGAACGACGGGGTGGACTAT‑GTGCCGACCGACACGAAAGTGCGGTTAGGTGA‑CCATTTTGCGGc                                                                                                                                                                                                                              >  2:946912/1‑238 (MQ=255)

                        tGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGAAGCGTATGTCGCCAGCCGGGCGCCATAACGCCGGGCTGGACTATGGGGCCGA‑GGACAAGGACGAGCCGTTTGGTCA‑CCATTTTggggg                                                                                                                                                                                                                              >  2:986262/1‑237 (MQ=255)

                           gAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGGGGGCCCTAACGACGGGGTGGACTAT‑GTGCCGCCGGGCAAGGAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCCtt                                                                                                                                                                                                                          >  1:19882/1‑239 (MQ=255)

                           gAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGAGCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACGCGTCGCGGTGGACGCCCGCGGATGGCCATAACGACCGGCTGGCATCA‑GTGGCGCCCAGCAAGAAAGGGATGTTCGGTCA‑CCACTTTGCGGcgcgt                                                                                                                                                                                                                          >  2:671773/1‑235 (MQ=255)

                             gCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAGAATGTGCTGGCGGTTGACCCGACGCGTATGGCGCCAGCCGTGCGCCATAACCAGGGGGTGGACTCT‑GTGCCGACGGGCAAGAAAGGGCTGTTGGGTAACCCATTTTgggcccttc                                                                                                                                                                                                                          >  2:80552/1‑230 (MQ=255)

                             gCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGGCCCGCCGCGGATGGCCCCTGCCGGGGGCCATAACGACGGGCTGGACCAG‑GTGCCGACGGACCAGGAAGGGCTGTGCGGGCG‑CCCTTTTGCGGCCATTg                                                                                                                                                                                                                         >  2:502554/1‑238 (MQ=255)

                             gCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGGCCCGAACCGTATGACGCCAGCGGGTGGCACTAATGGCGGGCTGGGAAAT‑GTGCCGACGGGCCAGAAAGGGCTGTTCGGCCA‑ACATTTTGCGGGCATTg                                                                                                                                                                                                                         >  2:894421/1‑238 (MQ=255)

                             gCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTg                                                                                                                                                                                                                         >  1:407776/1‑238 (MQ=255)

                             gCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTg                                                                                                                                                                                                                         >  1:446132/1‑238 (MQ=255)

                               aTTTGCTCTGGGATCCATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGCCGCCAGCGGTGCGCCATAACGACGGGCTGGACTCT‑GTGCCGCCGGACACGAAAGTGCTTTTCCGTCA‑CCATTTTTCCGGCATTGTCg                                                                                                                                                                                                                      >  1:627239/1‑239 (MQ=255)

                               aTTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGATGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGAATAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCg                                                                                                                                                                                                                      >  1:629765/1‑239 (MQ=255)

                               aTTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGAGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCg                                                                                                                                                                                                                      >  1:1068206/1‑239 (MQ=255)

                                tttGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGGCCCGACGCGTATGGCGCCACCGGTGCGCCACAACGGCGGGCGCGGCTCT‑GCGCCGCCGGGCCAGGAAGGGCTCTTTGGGCT‑CCAGTTTGCGGCGCATGCCgg                                                                                                                                                                                                                     >  2:1144208/1‑239 (MQ=255)

                                           aTACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAGAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCg                                                                                                                                                                                                          <  1:80552/238‑1 (MQ=255)

                                               aTTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCtggt                                                                                                                                                                                                     <  1:703813/239‑1 (MQ=255)

                                                   cTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGATGACCCGACGCGTATGACGCCTGCGGGGCCCCCTAACGACGGGCTTGGCTAT‑GTGGCGCCGGCCGACCAACGGCTGCTTGGGAA‑CCCTTTTTCGGCCCATGCCGGAG‑GCGGATCGGTGGTgggt                                                                                                                                                                                                 >  2:720983/1‑238 (MQ=255)

                                                   cTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTATGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGCCGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGGG‑CAGGTCCGCTGGTggg                                                                                                                                                                                                  >  1:616945/1‑238 (MQ=255)

                                                    tttAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTgggg                                                                                                                                                                                                 <  2:462043/238‑1 (MQ=255)

                                                          tCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑c                                                                                                                                                                                                                 <  1:1121570/216‑1 (MQ=255)

                                                             tGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGGCCCTTCCGGGATGGAGGCAGCGGTGCGCCCTATCGGGGGGCTGGGATAG‑GGGCCGACGGCAACGGAAAGGCCGTTCGGTGC‑CCATTTTGAGTCCATTGGCGGCGACAGGCGCG‑TGGTGGGGG‑CGGTAT‑Tgg                                                                                                                                                                                     >  2:576434/1‑239 (MQ=255)

                                                             tGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGGCGCGGATGACGCCAGCGGGGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGGGCTGTTCGGTCA‑CCATTTTGCGGCCCTTGCCGGGG‑CAGGGCCGCGGGGGGGGC‑CGCGAC‑Tgg                                                                                                                                                                                     >  2:204631/1‑239 (MQ=255)

                                                             tGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGGGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGGGGGGG‑CGGTAC‑Tgg                                                                                                                                                                                     >  1:1138783/1‑239 (MQ=255)

                                                                     aGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCaa                                                                                                                                                                            <  2:703390/239‑1 (MQ=255)

                                                                          aaCGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑ggg                                                                                                                                                                      <  2:370534/239‑1 (MQ=255)

                                                                            cgcgCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGCTGACCCGACGCGGATGACGCCAGCCGTGCGCCATAACGACGGGCTGGACTAT‑ATGCCGACGGACAAGAAAGAGCTGTGCGGTGG‑CCATTTTCGGGCCCTTGACGGCG‑CACGTCGGCAGGTGGGGC‑Cgctgcgcggggcggcaacgggcc                                                                                                                                                                       >  2:50242/1‑217 (MQ=255)

                                                                               gcTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTTGCCCGACGCGTATGACGCCAGCGGGGCGCCATAACGCCGGGCTGGACTAT‑GTGCCGACGGACCAGGAAGGGCCGTTCGGGCA‑CCATTTTGCGGCCAATGCCGGGG‑CCGGTCCGGCGGGGGGGC‑CGGGCCTTGTCGG‑CGCCAAT‑ggggac                                                                                                                                                                   >  2:433455/1‑235 (MQ=255)

                                                                                 tgtgGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGGGGGGC‑CGGTAC‑GTGCGG‑CGCCAAT‑GGGCTACCt                                                                                                                                                                >  1:855302/1‑238 (MQ=255)

                                                                                 tgtgGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGAATAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGGGGGGC‑CGGGAC‑TGGCGG‑CGCAAAA‑GGGCTACCt                                                                                                                                                                >  1:21617/1‑238 (MQ=255)

                                                                                  gtgGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑Gc                                                                                                                                                             <  1:422685/239‑1 (MQ=255)

                                                                                   tgGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCTAAT‑GGGCTACCT‑Gcc                                                                                                                                                            >  1:969417/1‑239 (MQ=255)

                                                                                      aTTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAGAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGGGGGGC‑CGGTAC‑TGGCGG‑GGCAAAA‑GGGCTACCT‑GCCggg                                                                                                                                                         >  1:524906/1‑239 (MQ=255)

                                                                                          tggtggCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGAAGCCCGCGGGGCGCCATAACGCCGGGCTGGACTAT‑GTGCCGGCGGACAAGAAAGGGCGGTTTGGTCA‑CCCTTTTGCGGCCCTTGCCGGGG‑ACGGGCCCCTGGGGGGGC‑CGGGAT‑TGGCGG‑CGCCCAA‑GGGGCACCTGGCCgggggt                                                                                                                                                      >  2:1030646/1‑237 (MQ=255)

                                                                                             tggCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCCGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGAAGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGggtga                                                                                                                                                    >  1:423536/1‑237 (MQ=255)

                                                                                                   cgttcgttatctatcGTATCGCTTACCGTTTTTAGGGGCTGTATATCGCCAAAAAGGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGgct                                                                                                                                             <  2:941029/231‑1 (MQ=255)

                                                                                                   cGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTGCCT‑GCCGGGGATGATCTGgctg                                                                                                                                            <  1:331004/239‑1 (MQ=255)

                                                                                                   cGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGgct                                                                                                                                             <  1:323958/238‑1 (MQ=255)

                                                                                                    ggTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCTGCGCGGATGGCGCCCGCGGTGCGCCATAACGACGGGGTGGACTAT‑GGGCCGGCGGGAAAGAGAGTGCTGTTCGGGCA‑CCATTTTGCGGCCGGTTCCGGGG‑ACGGTACGGTGGGGGGGCGCGGCA‑‑TGGCGGCCGCAACG‑GGGGT‑CCT‑GCCgggggggcgcggggtg                                                                                                                                            >  2:641880/1‑226 (MQ=255)

                                                                                                        tGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGc                                                                                                                                       <  1:650858/239‑1 (MQ=255)

                                                                                                            tctatctGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTggg                                                                                                                                   <  1:856655/239‑1 (MQ=255)

                                                                                                             ctatctGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTgggg                                                                                                                                  <  1:986262/239‑1 (MQ=255)

                                                                                                                tctGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGGTGCTCGCTGgggggg                                                                                                                               >  1:201593/1‑239 (MQ=255)

                                                                                                                    aTCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTTCGCCATAACGACGGGCTGGAATAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAA‑GGGCTCACT‑GCCGGGGGTGATGGTGGTGCTCGCCGGGGGGGttt                                                                                                                            >  2:1084697/1‑237 (MQ=255)

                                                                                                                    aTCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACCAGAAAGGGCTGTTCGGGCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGGCCGCTGGTGGGGC‑CGGGAC‑TGGCGG‑CGCCAAT‑GGGGGACCT‑GCCGGGGGTGGGCTGGGTGCTCGCTGGGGGGGGtc                                                                                                                            >  2:919339/1‑238 (MQ=255)

                                                                                                                          tACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCCAAT‑GGGCTACCT‑GCCGGGGATGGTCTGGCTGCTCGCTGGGGTGGTTCTCG‑Ccgg                                                                                                                    >  1:1022519/1‑239 (MQ=255)

                                                                                                                            ccGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑Ccggtg                                                                                                                  <  1:750747/239‑1 (MQ=255)

                                                                                                                            ccGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑Ccggtg                                                                                                                  <  1:946912/239‑1 (MQ=255)

                                                                                                                            ccGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑Ccggtg                                                                                                                  <  2:1160199/239‑1 (MQ=255)

                                                                                                                            ccGCTTTTATGGGCTGTAGTCCGCCAAAAAGGTGCTGGCGGTTGGCCCGACGTGTAGGGCGCCAGCGGTGCGCCTTTCCGCCGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTGGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGGTGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑Ccggtg                                                                                                                  <  2:717538/239‑1 (MQ=255)

                                                                                                                              gTTTTTAGGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑Ccggtgcg                                                                                                                <  2:407776/239‑1 (MQ=255)

                                                                                                                                   tATGGGCTGTATATCGCCAAAAATGGGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCa                                                                                                           <  1:988875/239‑1 (MQ=255)

                                                                                                                                   tATGGGCGGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCa                                                                                                           <  1:657064/239‑1 (MQ=255)

                                                                                                                                                tCGCCAAAAATGTGCTGGCGGTTGGCCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTg                                                                                              <  1:561969/239‑1 (MQ=255)

                                                                                                                                                 cGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGGTGGTCTGGCTGCTCGCTGGGGTGGGTCTCG‑CCGGGGCGGTGCAGGGTTTCATGGt                                                                                               >  1:886466/1‑237 (MQ=255)

                                                                                                                                                 cGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGGGCGCCATAAAGACGGGCTGGACCAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCCGCG‑CAGGGCCGCTGGTGGGGC‑CGGGCA‑TGGCCG‑CGCAAAT‑GGGGTACCT‑GCCGGGGGAGGTCTGGGTGCTCGTCGGGGGGGTTTTCG‑CCGGGGGGGTGGCGGGTTTCATGGGGc                                                                                             >  2:793401/1‑239 (MQ=255)

                                                                                                                                                  gCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTg                                                                                              <  1:1049730/237‑1 (MQ=255)

                                                                                                                                                   ccAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCCGCGGTGCGCCATACCGCCGGGCTGGACTAT‑GTGCCGACGGACACGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAA‑CAGGTCCCCTGGTGGGGC‑CGGTCC‑TGGCGG‑CCCACAT‑GGGCTGCCT‑GCCGGGGGTGGTCTGGGTGCTCGTCGGGGGGGTGTTTG‑CCGGTGCGGTGGCGGCTTTTGTGGTGCtc                                                                                           >  2:649154/1‑238 (MQ=255)

                                                                                                                                                      aaaaTGTGCTGGCGGTTGACCCGGCGCGTATGACGCCAGCGGGGGGCCATAACGACGGGCTGGACTAT‑TGGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑AAGGTCCGCTGGTGGGGC‑CGGTAC‑CGGCGG‑GGCAAAA‑GGGGCACCC‑GCCGGGGGGGGTGTGGGGGGTCGCCGGGGGGGTTTCCG‑GCGGGGCGGTGGCGGGTTTCAGGGGGCtggtt                                                                                        >  2:518732/1‑239 (MQ=255)

                                                                                                                                                      aaaaTGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCCAATGGGGCCCCGT‑GCGGGGGGGGTTC‑GGCTGCTCGCCGGGGGGGGTTGCG‑Ccggtgcgggggggggtctcccgggggtgtgt                                                                                        >  2:862695/1‑216 (MQ=255)

                                                                                                                                                      aaaaTGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGGTGATCTGGGTGCTCGCTGGGGTGGTTCTTG‑CCGGGGCGGTGCAGGATTTCATGGGGCtggtt                                                                                        >  1:933859/1‑239 (MQ=255)

                                                                                                                                                      aaaaTGTGCTGGCGGTTGACCCGACGCGTATGAAGCCAGCGGTGCGCCATCACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGGGCTGTTCGGGCA‑CCATTTTGCGGCCATTGTCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGGAA‑TTGCGG‑GCCCAAA‑GGGGTCCCC‑GCCGGGGGGGATCTGGGGGATCGCCGGGGTGGTTGCCG‑CCGGGGCGGGGCCGGGTTTACTGGTGCtgttt                                                                                        >  2:573638/1‑239 (MQ=255)

                                                                                                                                                              cTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCCCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTTCGGCCATTGCCGGAG‑CAGGTCCGGTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCCAAT‑GGGCGCCCC‑GCCGGGGGTGGTCTGGCTGCCTGCTCGGGGGGGTCGCGCCCGGTCGGGGGCGGGTTTTCTGGTTGTTgtttgtttgtc                                                                                 >  2:1187084/1‑236 (MQ=255)

                                                                                                                                                                       gACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGa                                                                       <  1:1003964/239‑1 (MQ=255)

                                                                                                                                                                           cGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGt                                                                   <  1:831767/239‑1 (MQ=255)

                                                                                                                                                                             aCGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtc                                                                  >  1:778960/1‑238 (MQ=255)

                                                                                                                                                                               gcgTATGACGCCGGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgct                                                               <  1:681039/239‑1 (MQ=255)

                                                                                                                                                                               gcgTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCCTGGTGCTGTTTGGTTCTACGCGCCGTGCAGGtcgc                                                                >  2:485531/1‑238 (MQ=255)

                                                                                                                                                                                 ggaTGACGCAGGGGGGGCGCCCTAACGGCGGGCGGGACTGT‑GGGCCGACGGAGAAGAAAGGGCTGTTCGGTCA‑CCATTTTGCGGCGATGGCCGGAG‑CAGGGCCGCTGGGGGGGC‑CGGTTC‑GGGCGG‑CGCAAGT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgctc                                                              <  2:627239/236‑1 (MQ=255)

                                                                                                                                                                                 gTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATGGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgctcg                                                             <  1:432149/239‑1 (MQ=255)

                                                                                                                                                                                   aTGACGCAATGGGTGCGCCAGAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCG‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTcgctcgct                                                           <  2:1068206/239‑1 (MQ=255)

                                                                                                                                                                                      gcggcAGCGGGGGGCCATAACGGCGGGCGTGACTAT‑GGGCCGGCGGGCAAGAAAGTGCGGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTgg                                                         <  2:524906/234‑1 (MQ=255)

                                                                                                                                                                                          cAGCGGTGCGCCATAGCGACGGGCTGGTGTAG‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAg                                                    <  2:96096/239‑1 (MQ=255)

                                                                                                                                                                                          cAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAg                                                    <  1:894421/239‑1 (MQ=255)

                                                                                                                                                                                                  cgcCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGGCGGTCGCTCGCTGGGTGGGCTGGTCag                                            >  2:634647/1‑238 (MQ=255)

                                                                                                                                                                                                  cgcCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGGTCTCG‑CCGGTGCGGTGCAGGATTTCCTGGTGCTGTCTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGGGCTGGTCaa                                            >  1:1037068/1‑239 (MQ=255)

                                                                                                                                                                                                  cgcCATAACGAAGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCa                                             <  2:671047/238‑1 (MQ=255)

                                                                                                                                                                                                     cttAACGCGGGGCTGGACTGT‑GTGCCGGCGGACAAGAAAGGTCTGTTGGGTCC‑CCATTTGGCGGCCATTGCGGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTCCCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAaag                                          <  2:446132/236‑1 (MQ=255)

                                                                                                                                                                                                       tAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGGTCTGGCTGCTCGCTGGGGGGGTTCTCG‑CCGGTGCGGTGCAGGATTTTCTGGGGCTGTTTGTTTCTACGCCGCGTGGCGGGCGGTCGCTggggggggtgggcgaaggag                                       >  2:18332/1‑222 (MQ=255)

                                                                                                                                                                                                               ggCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                                >  1:527090/1‑238 (MQ=255)

                                                                                                                                                                                                                  tGGACTGG‑GTGCGGACGGACAGGAGAGTGCGGTGCGGTCA‑GTGTTTGGGCGCCATGTCGGGCT‑CGGGGGGGCTGGTGGGCT‑GGGTGC‑TGGCGG‑CGCAATT‑GGGGTACCT‑GCCGGGGATGGTTGGGCTGCTCTTTGTGTTGGTTCTCG‑CCGGTGCGGGGCAGGATTTCTGGGTGCTGTTTGTTTCTACGCGCCGTGACGGCCGCTCGGGGGGTGAGCTGGTCAAAGAAGAGGTGGGGcc                             <  2:616945/238‑1 (MQ=255)

                                                                                                                                                                                                                  tGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGcc                             <  1:862695/238‑1 (MQ=255)

                                                                                                                                                                                                                       tgt‑gtgCCGAGGGACAAGAAAGGGCGGTTGGGTCA‑CCATTTTGCGGCCATTGCCGGGG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑GGGCGG‑CGCAAAG‑GGGCTCCCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCGGTTTGTTTCGACGCGCCGTGACGGTCGCTCGCTGGGGGAGCTGGTCAAAGAAGAGATGGGGCCAAccgc                       <  2:19882/237‑1 (MQ=255)

                                                                                                                                                                                                                         t‑gtgCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgccg                     >  1:317430/1‑239 (MQ=255)

                                                                                                                                                                                                                         t‑gtgCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGGGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgccg                     >  1:849610/1‑239 (MQ=255)

                                                                                                                                                                                                                               cGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGGTGGGGTGGTTCTCG‑CAGGTGCGGAGCAGGATTTGATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTg                >  1:1081764/1‑239 (MQ=255)

                                                                                                                                                                                                                               cGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTg                >  1:80787/1‑239 (MQ=255)

                                                                                                                                                                                                                                    gACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGAGGTTCTCG‑CCGGTGCGGTGCAGGATTTGATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTTCACGACGAGATGGCGCCAACCGCCAGGGCGATTgc           >  2:156303/1‑239 (MQ=255)

                                                                                                                                                                                                                                    gACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCG‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑ACGGTGCGGGGCAGGATTTCATGGTGCTGTTTGTTGCTACGCGCCGTGACGGTCGCTCGCTGGGTGATCTGGTATATGAAGAGGTGGGGGCAGCAGCGGGGGTGATTgc           >  2:326474/1‑239 (MQ=255)

                                                                                                                                                                                                                                            aGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCtggtg    >  1:1120241/1‑238 (MQ=255)

                                                                                                                                                                                                                                              tGCTGTTCGGTCA‑CCATTTTGCGTCCATTGCCGGCG‑CAGGGCGTGTGGTGGGGC‑CGGTAC‑TGGGGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTGGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGGGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGc  <  2:969417/238‑1 (MQ=255)

                                                                                                                                                                                                                                              tGCTGTTCGGTCA‑CCATTTTGCGGCCATGGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGc  <  1:1187084/238‑1 (MQ=255)

TCGTCTGGACAGTGCTCTCTGTAATGGGAGCATTTGCTCTGGGATACATTGCTTTAAATCGTGGGGAACAGATCAACGCGCTGTGGATTGTGGTGGCGTCGGTCTGTATCTATCTGATCGCTTACCGTTTTTATGGGCTGTATATCGCCAAAAATGTGCTGGCGGTTGACCCGACGCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTAT‑GTGCCGACGGACAAGAAAGTGCTGTTCGGTCA‑CCATTTTGCGGCCATTGCCGGAG‑CAGGTCCGCTGGTGGGGC‑CGGTAC‑TGGCGG‑CGCAAAT‑GGGCTACCT‑GCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCG‑CCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGC  >  NC_000913/629916‑630390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`