| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 989,890 | T→G | 22.2% | D156A (GAC→GCC) | pncB ← | nicotinate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 989,890 | 0 | T | G | 22.2% | 84.7 / 3.0 | 56 | D156A (GAC→GCC) | pncB | nicotinate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (11/25); new base G (9/4); total (23/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.22e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGTCGACTAATTTGCTTTCCAGC‑GTGTCGAGGGCTTG‑CGCAACGTCGGCCTGCGGTGAGCGATAGCG‑ATGTA‑CCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGT > NC_000913/989662‑990109 | tCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGTCGACTAAt < 2:1172103/237‑1 (MQ=255) gggCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGTCGACTAATTTGCt < 1:234480/239‑1 (MQ=255) aTTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTTAA‑GCCGGGGAATCGGCTAATTTTCttttcc > 1:1030287/1‑235 (MQ=255) aTTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGCTAAC‑GCCACGAAGTCGGCTAATTTTCTTTccc > 1:59029/1‑237 (MQ=255) ttGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGTCGACTAATTTGCTTTCCAGC‑g < 2:158107/239‑1 (MQ=255) gCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGTCGACTAATTTGCTTTCCAGC‑GTGTCGAGGGc < 1:91808/239‑1 (MQ=255) gCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGTCGACTAATTTGCTTTCCAGC‑GTGTCGAGGGc < 1:813202/239‑1 (MQ=255) ccTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGACAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGTCGACTAATTTGCTTTCCAGC‑GTGTCGAGGGc < 1:92137/238‑1 (MQ=255) ttCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGAAATATCAAGACCGGCGGTTAAA‑GCCGAGAAAGCGGCTAATTTGGTTTTCAGCGGTGGCGAGGG‑TTA‑CGCAAccg > 1:496772/1‑237 (MQ=255) ttCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGGTTCTGGTGCCCACAAACCAGGATTCCTGTTGCACACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCACGACCGGCGGTTCAA‑ACCCAGACATACACTACTTTTCTTTCCAGG‑GTGTCGCGGCCTTt‑cgccccgt > 2:166748/1‑234 (MQ=255) gtgtTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTTAA‑ACCGCCAAGTGGGAAAAATTGTTTTCCCAC‑GCGTCGGGGGCATg‑cggcaccgccgcccgtggg > 2:838957/1‑222 (MQ=255) tgtTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAATCGACTAATTTGCTTTCCCGC‑GTGTCGGGGGGTTG‑GGGAACGCCGGccccggggg > 1:257671/1‑232 (MQ=255) gtTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGTCGACTAATTTGCTTTCCAGC‑GTGTCGAGGGCTTG‑CGCAACGTCGGCCTGCGGTg < 2:774788/238‑1 (MQ=255) ttCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGTCGACTAAt < 1:637580/191‑1 (MQ=255) ccgtCGGCGCGAGGGAAAGCCGACGCGCCAGTTCGTAGTTGCTTGTGCCCCGAAACCAGGATTCCTGTTGCGGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAACGCGGCGACGGGTGCCAATATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGTCGACTAATTTGCTTTCCAGC‑GTGTCGAGGGCTTG‑CGCAACGTCGGCCTGCGGTGagcga < 2:1051987/236‑1 (MQ=255) ccacgggCGGGAGGGAAAGCCGACGCGCCAGAACGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTGGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGGCGACTAATTTGCGGTCCAGC‑GTGTCGAGGGCTTG‑CGCAACGTCGGCCTGCGGTGagcga < 2:336952/234‑1 (MQ=255) ccATCGGCGTGACGGGAAGCCGGCGCGCCAGAGCGCAGTTGCTGGTGCTCACAAACCAGGATTCCTGTCGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGTCGACTAATTTGCTTTCCAGC‑GTGTCGAGGGCTTG‑CGCAACGTCGGCCTGCGGTGagcga < 2:168200/239‑1 (MQ=255) aTCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGGTAAA‑GCCCAGAAAGCGACAAATTTTCTTTTCAGC‑GTGGCGGGGGGTTG‑GGCCAAGTCgggcctcggggaggggta > 1:383272/1‑223 (MQ=255) ggCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGGCGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑CCCCGGAAGACGGCTAATTTGCCTTTCCGC‑GGGGTGGGGGGTTG‑CGCCACGTCGGCCCCCGGGGagcgataacg > 1:438034/1‑239 (MQ=255) ggCGTGAAGGAAAGCCGACTCGCCAGAACGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGTCGACTAATTTGCTTTCCAGC‑GTGTCGAGGGCTTG‑CGCAACGTCGGCCTGCGGTGagcgatagcg < 1:616190/239‑1 (MQ=255) ggCGGGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCCAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTTAA‑GACAGCAAGGCGCCGAACTTGTTTTTCATA‑GCGTGGAGGGGTTG‑CGCCACGGCGCGCGGCGGTGggcgctgacg > 2:1176973/1‑233 (MQ=255) cGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGTCGACTAATTTGCTTTCCAGC‑GTGTCGAGGGCTTG‑CGCAACGTCGGCCTGCGGTGagcgatagcg‑a < 1:976188/238‑1 (MQ=255) gggAGGGAAAGACGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGTCGACTAATTTGCTTTCCAGC‑GTGTCGAGGGCTTG‑CGCAACGTCGGCCTGCGGTGAGCGATAGCG‑ATg < 1:673649/237‑1 (MQ=255) gAGGGAAAGCCGACGCGACAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAAGCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGTCGACTAATTTGCTTTCCAGC‑GTGTCGAGGGCTTG‑CGCAACGTCGGCCTGCGGTGAGCGATAGCG‑ATGtt < 1:496940/239‑2 (MQ=255) aGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGTCGACTAATTTGCTTTCCAGA‑GTGTCGAGGGCTTG‑CGCAACGTCGGCCTGCGGTGAGCGATAGCG‑ATGTa < 1:75419/238‑1 (MQ=255) cGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTACAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGTCGACTAATTTGCTTTCCAGC‑GTGTCGAGGGCTTG‑CGCAACGTCGGCCTGCGGTGAGCGATAGCG‑ATGTA‑CCA‑TTTCAc < 2:831923/238‑1 (MQ=255) agagCGTAGTTGCGGGTGCCCACAAACCAGGATTCCTGTGGCGAGCGCTTAACATTGGTTTCTTGTACTTC‑GGGAGAAAAACGGGGACGGGTGCCAAAATCC‑ATAAGAGGGAAGCGCGACATATAAAGACCGGCGGTTAAC‑GCCGAGAAGTCGACTAATTTGCTTTCCAGC‑GTGTCGAGGGTTTG‑CGAAACGTCGGCCGGCGGGGAGCGATAGCG‑ATGTC‑CCA‑TTTCACTGATAACCg < 2:95167/238‑1 (MQ=255) ggTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAA‑ACCGAGAAGGCGACTAATTTGCTTTCCAGC‑GGGTCGGGGGGTTG‑CGTGACGGCGGCCCGCCGTGGTCGGTAGGG‑GCGCA‑CCA‑TTTTCATGATAAACGCCAGCACGGCAACtt > 2:509553/1‑239 (MQ=255) aCCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGTCGACTAATTTGCTTTCCAGC‑GTGTCGAGGGCTTG‑CGCAACGTCGGCCTGCGGTGagcgat < 1:1129982/186‑1 (MQ=255) gATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAA‑CCCCAGAAAACGACTAATTTGCTTTTCAGC‑GCGGCCGGGGCTTG‑CGCAACGTCGGCCCGCGGTAAGCTGTAGCG‑GTGTACCCA‑TTCCAC‑GATGACCTCCTCCAACAGGACTCACCAGAGGAGGACtt > 2:406889/1‑238 (MQ=255) cTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGTCGACTAATTTGCTTTCCAGC‑GTGGCGAGGGCTTG‑CGCAACGTCGGCCCGCGGTGAGCGATAGCG‑ATGGA‑CCA‑TTTCACTGATAACCCCCAGCAAAGGAACTTCCCAGAGGGAGACTTCACGc > 1:934156/1‑238 (MQ=255) gTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGTCGCCTAATTTTCTTTTCAGC‑GTGTCGGGGGGTTG‑CGCAAAGTCGGCCTGGGGGGGGCGATAGCG‑GTGTA‑CCA‑TTTCACTGATAACCGCCACCAAAGGAACTTCCCAAGGGATGACTTCCCGcccc > 1:90289/1‑237 (MQ=255) gCAGACGCTAAACGATGGTTTCTGGTACTGC‑GCGAGAAAACCGGGGACGGGGGCAAAAATCC‑ATCAGAGGGAGGCGCGACATATCAGGACGGGGGGTAAAC‑GCGGAGAGGTCGTCTAATTTGCTTTCCGGC‑GTGTCGAGGGCTTG‑CCAACCGCCGGCCGGAGGGGGGAGATAGCG‑AGGTA‑CAT‑TTTCACGGAGAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACggg < 2:438034/239‑1 (MQ=255) aGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGTCGACTAATTTGCTTTCCAGC‑GTGTCGAGGGCTTG‑CGCAACGTCGGCCTGCTGTGagcgata < 1:207612/170‑1 (MQ=255) aGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAA‑GCCGAGAAGTCGACTAATTTGCTTTCCAGC‑GTGTCGAGGGCTTG‑CGCAACGTCGGCCTGCTGTGagcgata > 2:207612/1‑170 (MQ=255) ttAAGGTTGGTTTCTGGTACTAG‑GCGAAAAAAAGGGGGA‑GGGTGGCAAAAACCAATCAGAGGGAGGGGCTAAATAACAAGACGGGGGGTGACC‑CCGTAGAAGTCGACTATGTTGATTTCCGGG‑GGGCGGGGGGTTGG‑CGCAACGTCGGCCTGCGGTGGAGGTTAGCG‑TGGTA‑CCA‑TTTCACTAATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTa < 2:341389/238‑1 (MQ=255) taaaggATGGTTTCTTG‑ACATCGGCGAGAAAAACGGGGCGGGGTGCAAAAATCC‑AACAGGTGGAAGAGCGAAATATCAAGACCGGGGGTTAAC‑GCGGAAAAGGCGACTAATTTGTTTCCCAGC‑GTGTGGAGGGTTTG‑CGAAACGCCGGCCGGCGGTGGGCGATAGCG‑AGGTA‑CCA‑TTTCCCGAAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTa < 2:383272/233‑1 (MQ=255) ggTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGGCGACTCAATTTCTTTCCAGA‑GGGGCGGGGGGCTG‑CGCAAAGCCGGCCTGCGGGGAGCGGAAGCG‑AGGTA‑ACA‑ATTTTCTCATAAACCCCAGCAAAGGGACTTCCCACAGGAGGGATTTACTCCACGGGCCCCTTTAAAGaaaa > 2:981972/1‑236 (MQ=255) tttCTTGTCCTCC‑GGAAAAAAACCGGCGCGGGGGGCAAAAACCC‑ATCAGATGGAAGCGGGAAATACAACGACCGGCGTTTACC‑GCCGAGAAGCCGACTAATTTGCTTCCCAGG‑GTGTCGAGGGTTTG‑CGAACCGCCGGCCTGCGGTGGGCGTTAGCG‑ATTTA‑CCA‑TTTCCCGGAAACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATc < 2:1059599/238‑1 (MQ=255) tgttgcacggc‑gcgAAAAAACCGGCGAGGGGTGCAAAAACCC‑ATAAGATGGAAGCGCGACATACCAAGACGGGGGTTTAAC‑GCCGAGAAGGCGACTAATTTGCTTCCCGGC‑GTGTCGGGGGCTTG‑CGCAACGTCGGCCTGCGGTGAGCGATAGCG‑ATGTA‑CCA‑TTTCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAg < 2:1030287/229‑1 (MQ=255) aCTTC‑GCGAGAAAAACGGCGACGGGTCCCAAAACCC‑ATCAGATGGAAGCGCGACATATCAAGCCCGGCGTTTAAC‑GCCGAGAAGTCGACTAATTTGCTTTCCAGC‑GTGTCGAGGGCTGG‑CGAAACGTCGGCCTGCGGTGAGCGATAGCG‑ATGTA‑CCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGc < 1:838957/238‑1 (MQ=255) c‑gcgAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGGCGACTAATTTGCTTTTCAGC‑GGGTCGAGGGCTTG‑CGCAACGTCGGCCTGCGGTGAGAGATAGCG‑ATGTA‑CCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGATTGCCATTa > 1:858265/1‑239 (MQ=255) c‑gcgAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCCAGAAAGGGGATAAATTGCTTTCCAGA‑GGGTCGGGGGGTTGCCCCAACG‑CGGCCCGCCGGGGGGCATAGCG‑GTGGA‑CCATTTTCCCTATCAACCCCCCGAAAAGAACCTTCCC‑GGGGATTGATTCCCGCCCCCGGCCCCCTAAACGAATATCCaccctggccttt > 2:129666/1‑228 (MQ=255) c‑gcgAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGCTAAC‑GCCCAGAAGTCGACTAATTTGCTTTCCAGC‑GCGTCGGGGGCTTG‑CGCAAAGTCCGCCTGCCGTGAGCGATAGCG‑GTTGA‑CCA‑TTTCACTGATAACCGCCCGCAAAGGAACTTCCCCGAGGATGACTTCACACCCCCGGCCCCCTTAACGAATATCCCACCTTCCATTa > 2:176576/1‑239 (MQ=255) c‑gcgAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGATAAC‑GCCGGCAAGGCGACGAATTTGATTTCCACC‑GCGGCGGGGGGTCG‑CGGAAAGCCGGCCTGCGGTGTGGGGTAGAG‑GGGTA‑GCC‑TTTCACCGATAACCCCCCCGCAGGGAAAGTACCAAAGGGTGACTTTACGCCACCGACCGGCTAAACGAaaataactacaggcaatg > 2:589659/1‑225 (MQ=255) cgAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAA‑CCCGCGAAGACGACTACTTTGGTTTTCAGC‑GGGTCGGGGGCTTG‑CGCAACATCGGCCCTCGGGGAGCGATAGCG‑AAGGA‑CCA‑TTTTACTGCTAACCACCAGCCAAAGAACATCCCCCAGGAGGAATTCACGCCCACGGCAGCATAAAcaacgaatcatgtctcccacacg > 2:1191393/1‑217 (MQ=255) gataaagagcgcggcgGGTCCACAAATCC‑ATGCTATGGAAGGTCGACATAGCAAGCCCGGTGGTTAAC‑GCAGGAAAGCCGTCTTCTTCTCTTTCCAGG‑GTGTGGGGGTTGTG‑GCCACCGTCGGGTCGGGGGGGCCGATAACT‑ATGTC‑CCA‑TTACACGGATATCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTGGCCATTATCg < 2:742607/229‑1 (MQ=255) ggggcGGGTGAAAAAACCC‑ATAATAGGGAAGCGCGACATACAAAGCCTGGCGGTTAGC‑GCCGAGAGTCCGATTAGTGTGCTTTCCGGC‑GTGCCGAGGGGTGG‑CGCAACTCCGGCCGGGGGGGAGCGAT‑GCGAATGTC‑CCT‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACg < 2:779208/234‑1 (MQ=255) ggAAGCGCGACATATCAAGACCGGCGGATAAA‑GCCGCGAAATCGAATAATTTTCTTTCCAGC‑GTGTCGGGGGCCTG‑CGCAACGGCGGCCTGCGGTGAGCGATAGCG‑ATGTA‑CCA‑TTTTACTTATAACCGGCAGCAAAGGGACTTTCCAGAGGAAGACTTCAAGCCACGGCCCGACTAAACGAATATCCAACTTGCCCATATAGTTGGACCCTGTGGCTTTTTCCGGGTTAAAGCggc > 2:897926/1‑236 (MQ=255) aGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGGCGACTAATTTGCTTTCCAGC‑GGGTCGAGGGCTTG‑CGCAACGTCGGCCTGCGGTGAGCGATAGCG‑ATGTA‑CCA‑TTTCACTGATAAACGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAAAATCCAGCTTGCCCTTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTc > 1:1072813/1‑238 (MQ=255) aGCGCGACATATCAAGACCGGCGGTTAAA‑GCCCGGAAGTCGGCTAATTTTCTTTCCCGC‑GTGTCGAGGGCTTT‑CGCCACGTCGGCCTGCGGGGTGCGCTAGCG‑GTGTA‑CCA‑TTTCCCTGATAACCGCCCGCAAAGGAACTTCCCCGAGGAGGACTTTACCCCACCGGCCGCCTTAACGAAAACTCCGCCTGCCCTTATCTTTGTGCACGGGGACTTGTTCCAGGGTAAAGCgggaacc > 2:943205/1‑233 (MQ=255) ccATATAAGGAGCGGTGGTAAAC‑GCGGAAAATGCGCTTAATGTGCTTCCCGGG‑GTTTGGGGGCCTGG‑CGCAGCGTCGGCCGGCGGTGGGCAATGGCG‑ATGTA‑CCA‑TTTCCCGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa < 2:1087425/237‑1 (MQ=255) caaagaacaagccGGGGGGTAACGGCCGAGATGCCGAATAATTTGCTTTCGAGG‑GGTTCGAGGTCTTC‑GGCAACGTCGGCCGGGGGTGAGCATTAGGT‑GTGTA‑CCT‑TTCCCCGGATAGCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa < 2:576046/228‑1 (MQ=255) aCATATAAATCCCGGCGGTTACG‑GCCGAGAAGTCGACTAATTTGCTTTCCGGG‑GTGTCGGGGGCTTG‑CGCAACGGCGGCCTGCGGTGAGCGATAGCG‑ATGTA‑CCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa < 1:34652/238‑1 (MQ=255) tatCAAGCCGGGCGGTTACC‑GCCGAGAAGTCGCTTAATTTGTTTTCCGGC‑GTGTCGAGGGTTTG‑CAAAACGTCGGCCTGCGGTGAGCAATAGCA‑AGGTA‑CCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAg < 2:1072813/239‑1 (MQ=255) gcggTAAAC‑GCCGAGAAGTCGACTAATTTGCGTTCCAGC‑GTGTCGAGGGCTTG‑CGCAACGTCGGCCTGCGTTGAGCGATAGCG‑ATGTA‑CCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt < 1:347951/238‑1 (MQ=255) | TCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTC‑GCGAGAAAAACGGCGACGGGTGCCAAAATCC‑ATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAC‑GCCGAGAAGTCGACTAATTTGCTTTCCAGC‑GTGTCGAGGGCTTG‑CGCAACGTCGGCCTGCGGTGAGCGATAGCG‑ATGTA‑CCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGT > NC_000913/989662‑990109 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |