Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 343,444 A→G 20.5% G187G (GGA→GGG yahK → broad specificity NADPH‑dependent aldehyde reductase, Zn‑containing

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913343,4440AG20.5% 149.3 / 10.6 71G187G (GGA→GGGyahKbroad specificity NADPH‑dependent aldehyde reductase, Zn‑containing
Reads supporting (aligned to +/- strand):  ref base A (6/48);  new base G (13/2);  total (20/51)
Fisher's exact test for biased strand distribution p-value = 5.97e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACATGACCGGCACCTATAACTCGCCGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCTG  >  NC_000913/343209‑343681
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               
acaTGACCGGCACCTATAACTCGCCGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCCGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATCGGCGGGCTGGGGCat                                                                                                                                                                                                                                              >  1:410091/1‑239 (MQ=255)
  aTGTCCGGCACCTATAACTCGCCGACGCCGGACGAACCGGGCCATACTTTGGGCGGCTAGTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACatat                                                                                                                                                                                                                                            <  1:1196399/239‑1 (MQ=255)
  aTGACCGGCACCTATAACTCGCGCACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACatat                                                                                                                                                                                                                                            <  2:248698/239‑1 (MQ=255)
  aTGACCGGCACCTATAACTCGCCGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACatat                                                                                                                                                                                                                                            <  1:697044/239‑1 (MQ=255)
       cGGCCCCTATAACTCGCCGACGCCTGACGAACCGTGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGAGATGTTCTGCGTATGCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGa                                                                                                                                                                                                                                       <  1:640648/239‑1 (MQ=255)
         gCACCTATAACTCGCCGACTCCGGACGAACCGGTCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCCCCTTTGTTGTGTGGAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAGAGTGGGCGTGGGCGGCATCGGCGGTCCGGGTCCTCTGGGGagt                                                                                                                                                                                                                                     >  2:830186/1‑237 (MQ=255)
         gCACCTATAACTCGCCGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGGGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAACACGTGGGCGTGGTCGGCATCGGCGGGCTGGGAccatgcggggtg                                                                                                                                                                                                                                     >  1:1095589/1‑228 (MQ=255)
            ccTATAACTCGCCGACGCCGGACGAACCGGGCCATGCTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAg                                                                                                                                                                                                                                  <  1:68619/239‑1 (MQ=255)
             cTATAACGCGCCGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGAGCGTCGTTCATGGGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGAAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGa                                                                                                                                                                                                                                       <  1:474693/233‑1 (MQ=255)
              tataACTCGCCGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCt                                                                                                                                                                                                                                <  1:1012243/239‑1 (MQ=255)
                  aCTGGCCGCCGCCGGACGAACCGGGCCATACTCGGGGCGGCTACTCACAACAGAGCGTCGTTCATGAGCGATAGGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGcc                                                                                                                                                                                                                            <  2:86570/239‑1 (MQ=255)
                    tCGCCGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCa                                                                                                                                                                                                                          <  1:801275/239‑1 (MQ=255)
                    tCGCCGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGGCTGGGGCATATGGGGGGTAAGCAGAccc                                                                                                                                                                                                                           >  1:874160/1‑238 (MQ=255)
                       ccGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGGCTGGGACCTGTGGGGATTAAGcaaggcaacgc                                                                                                                                                                                                                       >  1:600686/1‑229 (MQ=255)
                       ccGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGCAAAAAAGTGGGCGTGGTCGGCATCGGCGGGCTGGGGCATATGGGGGTTAAAGTGGCCCAcgc                                                                                                                                                                                                                       >  1:346974/1‑239 (MQ=255)
                       ccGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACACGCAAGAGAAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCCGGGCTCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGCAAAAAAGTGGGCGGGGTCGGAATCGGCGGTCGCGGGCCTGTGGGGGGTAAGCCGGtcctc                                                                                                                                                                                                                         >  2:474693/1‑235 (MQ=255)
                           cGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGAt                                                                                                                                                                                                                    <  2:227772/238‑1 (MQ=255)
                           cGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGAt                                                                                                                                                                                                                    <  1:957067/238‑1 (MQ=255)
                           cGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGAt                                                                                                                                                                                                                    <  1:827773/238‑1 (MQ=255)
                           cGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGAt                                                                                                                                                                                                                    <  1:521526/238‑1 (MQ=255)
                                  cGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGGTGTGTGCAGGGATCAGCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATggggg                                                                                                                                                                                                               <  1:98468/236‑1 (MQ=255)
                                       cGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACAtgtg                                                                                                                                                                                                       <  2:1085117/239‑1 (MQ=255)
                                       cGGGCCATACTCGTGGCGGCTACTCCCAACAGAGCGTCGTGCATGAGCTTTTTGTTCGTCGTATGCGGCACCCGCAAGAGCAGCGGGCGGCGGTGGCTCCTTTGTTGGGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACAtgtg                                                                                                                                                                                                       <  2:991713/239‑1 (MQ=255)
                                          gCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGc                                                                                                                                                                                                              <  2:751866/229‑1 (MQ=255)
                                          gCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGc                                                                                                                                                                                                              >  1:751866/1‑229 (MQ=255)
                                               aCTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGCGCTGGGAATATTGGGGTTAAGGTGGGCCCAGCGGTGGGGGGCCATGggggggtgat                                                                                                                                                                                               >  2:1113255/1‑235 (MQ=255)
                                                    gggCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCATATGGGGATTTAGCTGGGCCCCGCGGTGGGGGCACATGGGGGGGCGttttac                                                                                                                                                                                           >  1:348422/1‑235 (MQ=255)
                                                     ggcggcTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGAATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGCGGGCGTGGTCGGCATCGGCGGGCTGGGGCCGATGGGGGGTAAGGTGGGCCCCGCGGTGGGGGGGCGGGTGGGGGCACTTagc                                                                                                                                                                                           >  2:582088/1‑235 (MQ=255)
                                                                               tcattggCGATATGTTCTGCGTATTCGGAGCCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTGGTTGTGTGCAGGGATCACCACGTATTCGCCGCTCCGGCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCaa                                                                                                                                                             <  2:1189708/233‑1 (MQ=255)
                                                                               tCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCaa                                                                                                                                                             <  1:244495/239‑1 (MQ=255)
                                                                                 aTGAGCGATATGTTCTGCGTATGCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCaaaa                                                                                                                                                           <  2:133285/239‑1 (MQ=255)
                                                                                       gATATGTTCTGCGTATTCGTCCCCCGCAAGAGCAGCTGGCGGGGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTAGTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCt                                                                                                                                                      <  1:1051754/238‑1 (MQ=255)
                                                                                        atcggTTCGGCGGATTCGTCACCCGCATGAGCAGCTGGCGGCGGTGGCTCCTTTGTGGTGTGCAGGGAGCACCACGTATGCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTg                                                                                                                                                     <  2:410091/234‑1 (MQ=255)
                                                                                            gTTCTGCGTTTTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGc                                                                                                                                                <  2:789627/239‑1 (MQ=255)
                                                                                             ttCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGa                                                                                                                                                                                                                                       <  1:544153/153‑1 (MQ=255)
                                                                                                 gCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATg                                                                                                                                           <  1:658647/239‑1 (MQ=255)
                                                                                                 gCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATg                                                                                                                                           <  1:243315/239‑1 (MQ=255)
                                                                                                       tCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTGCGGGGCATATGGGGGTTTAGCTGGCCCACCGGCGTGGGGGGCGTTGGGGGGCATTTATCACTTTCTGAGCCACAACCCGAGGGGGCAAAAACTCTGGGGCCCGTTGagttc                                                                                                                                      >  2:101581/1‑235 (MQ=255)
                                                                                                       tCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGGTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCCCTGGCAGGCCGGGCCGGGTAAAAAAGTTGGCGTGGTCGGCATCGGCGGGCCGGGGGCTATGGGGATTAAAACGGCCCCCGGCGCGGGGGCACACTCCGGGGGAGGTAACCCCTCTGGGGGAAACAGCGAAGCGGCGCAAAGCCCGGGGGGcacgttatgtg                                                                                                                                      >  2:56772/1‑229 (MQ=255)
                                                                                                            aCCCGCAAGAGCAGCTGGCGGCTGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGATGCTGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAAc                                                                                                                                <  1:751760/239‑1 (MQ=255)
                                                                                                               cGCAAGAGCAGCTGGCGGCGGTGGCTCCTTGGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACTCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCa                                                                                                                             <  1:948572/239‑1 (MQ=255)
                                                                                                                  aaGACCGGCTGGCGGCGGTGGCGCCTTTGTGGGTGGCAGGGATAACCACGTTTTCGCCGCTAGGTCACGGGGAGGCGGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACg                                                                                                                           <  2:492312/238‑1 (MQ=255)
                                                                                                                   agagCGGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGGATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCa                                                                                                                         <  2:710979/239‑1 (MQ=255)
                                                                                                                                     ggCTCCTTTGTTGTGTGCAGGGATCGCCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGTCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATggcgg                                                                                                       <  1:467031/239‑1 (MQ=255)
                                                                                                                                     ggCTCCTTTGTTGTGTGCAGGGATCACCACGTCTTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATggcgg                                                                                                       <  2:1045097/239‑1 (MQ=255)
                                                                                                                                      gCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATggcgg                                                                                                       <  1:1037796/238‑1 (MQ=255)
                                                                                                                                        tCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCt                                                                                                     <  1:2017/238‑1 (MQ=255)
                                                                                                                                                     gCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGGTTAAGCTGGCCCACGCGATGGGGGCACATGTGGGGGCATTTACCACTTCTGAGGCAAAACGCGGAGCGGCAAAAGCCCTGGGGGGCGATGGAGTTGTTAACTCACGCAATGACCATGAGATGGCGGCGCCTCTGAAAAGttt                                                                                       >  1:1019036/1‑239 (MQ=255)
                                                                                                                                                         ggATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTGTGGGGCCTTTGGGGGTTAAGCTGGGCCACGCGATGGGGGGACCTGGGGGGGCGATTACCACATTTGGGGCACAAAGGGGCACGGGAAAAGGACCCGGGGACGGTGAAGTTGTTAAATTCAGCAATGTCCATGATATggggggtgctttgaaaatattcggt                                                                                   >  2:272769/1‑219 (MQ=255)
                                                                                                                                                           aTCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTCAAGAGTTTCGAtt                                                                                  <  1:582088/238‑1 (MQ=255)
                                                                                                                                                            tCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTc                                                                                <  1:766126/239‑1 (MQ=255)
                                                                                                                                                              accacGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGGCTGGGGCCTATGGGGGTTAAGCTGGCCCACGCGATGGGGGGGCATGGGGGGGGATTTACCACTTCTGGGGCAAAACGCCAAGCGGCGAAAACCGTTGGGGCCGGTTATGTTGTTTAATCCCGACAAGCCGACAAGATGGGGGGCCCTCTGAAGAGGTTTGCTTTc                                                                                >  2:1093630/1‑237 (MQ=255)
                                                                                                                                                               ccacGTGTTGGCCGATCCGTCACTGGCAGGCGGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCAtt                                                                             <  2:547284/239‑1 (MQ=255)
                                                                                                                                                                 gcggactCGGCGGTACGGCAATGGGAGGGCGGGACGGGTAAAAAAATGGGCGTGGTCGGGATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTATGAGGCAAAACGCGAAGCGGCAAAAGCCGTGGGGGCAGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCAtttt                                                                           <  2:600686/233‑1 (MQ=255)
                                                                                                                                                                 acGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGGGGGTGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCAtttt                                                                           <  2:192734/239‑1 (MQ=255)
                                                                                                                                                                     atgcgccgcGACGTCACTGGGAGGCCGGGCCGGGTAAAAAAGTGGGCGCGGTCGGAATCGGCGGTCTTGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGaa                                                                        <  2:960480/235‑1 (MQ=255)
                                                                                                                                                                     aTTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCATATGGGGATTAAGCTGGCCCACGCGGTGGGGGCACATGTGGGGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGGAAAAGCCCTGGGGGCCGCTGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGAAt                                                                       >  1:142212/1‑239 (MQ=255)
                                                                                                                                                                     aTTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGaa                                                                        <  1:20489/238‑1 (MQ=255)
                                                                                                                                                                          ccgcTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTTTGGGGCATATGGGGGATAAGCTGGCCCACGCGATGGGGGCACACGTGGTGGCCTTTATCACTTCTGAGGCCAAACGCGACGCGGCAAAAGCCCTGGGGGCCGATGAGGTTGTTAACACACCGAATGACGATGAGATGGCGGGCCGACTGAAGAGGTTCGCTTTTACTTTGAAtctagt                                                                  >  2:216639/1‑234 (MQ=255)
                                                                                                                                                                                 gttcgtGGAAGGACGGGACGGGTCAAAAAGTGTGGGCGGTCGGCATAGGCGGTCTGGGAAATATGGGGATTAAGCTGGACCACGAGATGGGGGCACAAGTGGTGGCATTTAACACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCATGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGATATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTgcg                                                           <  1:475949/234‑1 (MQ=255)
                                                                                                                                                                                 gTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTgcg                                                           <  1:349526/239‑1 (MQ=255)
                                                                                                                                                                                              cgggccgggTAAAAAAGTTGGCGTGGTCGGCATCGGCGGTCTGTGTCATATTGGGATTAAGCTGGCCCACGCGCTGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCTGCAAAAGCCCTGGGGGCCGATGAAGTTGTTCACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTcg                                              <  2:874160/239‑1 (MQ=255)
                                                                                                                                                                                                 gccgggTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTcgac                                            <  2:347784/238‑1 (MQ=255)
                                                                                                                                                                                                 gccgggTAAAAAAGTGGGCGTGGTCGGCAGCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACGTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGGCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCAGTTTGAATACAGTAGCTGCGCTACAGAATCTcgac                                            <  1:168364/238‑1 (MQ=255)
                                                                                                                                                                                                       tAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTCGGGCATATGGGGATTAAGCTGGCCCACGCGCGGGGGGCACATGTGGGGGCATTTACCACTTTTGGGGCAAAACCCGGAGCGGCAAAAGCCCTGGGGGGCCGTGGAGTTGTTAACTCACGCAAAGCCGAAGAGATGGGGGCGCCCTTGAAAAGTTATGATTTTATTTTGAATTCAGAAGGGGCGCCACATAAACTcgcagtgttta                                     >  2:565559/1‑230 (MQ=255)
                                                                                                                                                                                                          aaaaGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTacca                                  <  1:830186/239‑1 (MQ=255)
                                                                                                                                                                                                          aaaaGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTacca                                  <  1:134268/239‑1 (MQ=255)
                                                                                                                                                                                                          aaaaGGAGGGGTGGGTGGCATCGGCGGTTTGGGGAATATGGTGACTAAGCGGGACCGCGCGACGTGGGCACATGTGTTGGCAGTTACCATTTCTGAGGCAAACCGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATATCGACGATTTTacca                                  <  2:11028/239‑1 (MQ=255)
                                                                                                                                                                                                                     ctggctggctgcgtggttCTGGGGTATATTGGTGTTAAGCTGGTACCTGGTATGGGAGCACGTGTAGGGGCATTTACCATTTCTGAGTTAAAACGCGAAGCGGCAAAAGCCATGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCGGAATAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCGACATAATCGCGACGATTTTACCACCTTGCTGa                         <  2:914070/221‑1 (MQ=255)
                                                                                                                                                                                                                             aTCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATgg               <  1:101581/239‑1 (MQ=255)
                                                                                                                                                                                                                                           gACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCtg  >  1:364794/1‑239 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               
ACATGACCGGCACCTATAACTCGCCGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCTG  >  NC_000913/343209‑343681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: