Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,877,629 C→T 20.9% intergenic (‑97/‑86) yoaF ← / → yeaP DUF333 family outer membrane lipoprotein/diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,877,6290CT20.9% 87.6 / 5.3 37intergenic (‑97/‑86)yoaF/yeaPDUF333 family outer membrane lipoprotein/diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (23/6);  new base T (2/6);  total (25/12)
Fisher's exact test for biased strand distribution p-value = 7.71e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAG  >  NC_000913/1877393‑1877856
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                   
gTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCt                                                                                                                                                                                                                                    <  1:453873/238‑1 (MQ=255)
                   aaTAGACCGACGCCGGATTTGCCATACCAATCTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTtgatg                                                                                                                                                                                                                 <  1:78911/238‑1 (MQ=255)
                           gACGCCGGATTTGCCATACCAATCTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTTACTCAAAACATATTACGTTTTGTTTTATCTTTGTTGATGATGtttttt                                                                                                                                                                                                        >  1:507760/1‑237 (MQ=255)
                                    tttGCCATACCAATCTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGc                                                                                                                                                                                               <  1:779109/239‑1 (MQ=255)
                                      tGCCATACCAATCTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCaa                                                                                                                                                                                             <  1:686325/239‑1 (MQ=255)
                                      tGCCATACCAATCGGCGGCGGGTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCATGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCaa                                                                                                                                                                                             <  1:373248/239‑1 (MQ=255)
                                                aTCTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCGGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAaata                                                                                                                                                                                   >  1:821236/1‑239 (MQ=255)
                                                  cTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAaataat                                                                                                                                                                                 <  1:280809/239‑1 (MQ=255)
                                                     cggcggTTTAGGTGGTTCTGGCTGAGAAGGGGTTGAACAAACAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATTGTTTTTTTTTTCAGCACTATTTTTTTTATATTCACTCAAAACATTTTACGTTTTTTTTTATCTTTTTTGTTTATGTTTTATTATGCCTTCAAAGATTAAATAATCAg                                                                                                                                                                              >  2:48059/1‑239 (MQ=255)
                                                               ggTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTAccc                                                                                                                                                                     >  2:1069397/1‑238 (MQ=255)
                                                                                       gAACAACCAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACTCGCCGTATCCTGGAGTTGTTCAgtt                                                                                                                                             >  2:491761/1‑237 (MQ=255)
                                                                                            acCAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGtcaga                                                                                                                                        >  1:1033996/1‑238 (MQ=255)
                                                                                             ccagccagGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGtcagatc                                                                                                                                      >  1:1193719/1‑239 (MQ=255)
                                                                                                          gcaagcaaGGCAGAACAAAACTGATACTTTTCATTTATTGATCTCACATATTTATCCCAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTTAATAATCAGCATTTACCCGCCGTATCCTGAAGTTGATCCGTGTCCGCTCAGATTATCGccc                                                                                                                          >  2:661955/1‑238 (MQ=255)
                                                                                                           caagcaagGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTAAATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATTCCTGCAAAGATTAAATAATCAGCGTTTACCCGCCGTATCCTGGAGTTGTTTCTTTTCAGATCAAATTTTCGCCcgc                                                                                                                        >  2:3798/1‑239 (MQ=255)
                                                                                                                  ggCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGc                                                                                                                 >  1:928201/1‑239 (MQ=255)
                                                                                                                         aaaatataatatTTTTATTTTTTTCATCTCACATATTAATCAAATATTAGAGTACCGGTTTTTTTTTTTTTTTTGCATTATTTTTTTTATAATTTTCAATAAAAATATATTACTTTTTTTTTCTTTTTTGTTGATTGTTTTTTATCCTGCATGAAAAAATAAAAAAATCAGTTTTTCCCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCt                                                                                                          <  2:507760/231‑1 (MQ=255)
                                                                                                                                 aTAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCg                                                                                                                  >  1:1133990/1‑223 (MQ=255)
                                                                                                                                    attttttttttttgacaccatttatTTATCAAAGATTAGAGTATCGCTGTATGTTTTTTTTTGGCAGTTTTTTTTTTATTATTTTCACAAAAAATATATAACTTTTTTATTCTTTTTTTTTTTTGTTTTTTTATCAGGCTTGAAATAATTAAATAATCACATTTTCCCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAAc                                                                                               <  2:543415/217‑1 (MQ=255)
                                                                                                                                                                     gATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAATTAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCTCCCGCGTCTCGCAATCTCTTTCCCAAGAACAGTCACTGGCAAGTCTGGTCCGACCGCTTCTg                                                               >  2:1182522/1‑238 (MQ=255)
                                                                                                                                                                     gATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTgg                                                              >  1:83358/1‑239 (MQ=255)
                                                                                                                                                                     gATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTgg                                                              >  1:484585/1‑239 (MQ=255)
                                                                                                                                                                               tCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGa                                                    >  1:236405/1‑239 (MQ=255)
                                                                                                                                                                               tCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGa                                                    >  1:694857/1‑239 (MQ=255)
                                                                                                                                                                               tCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAAATACTGGa                                                    >  1:897040/1‑239 (MQ=255)
                                                                                                                                                                                cgcgGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAACTCTGGTCCGACAGCTTCTGGCGATGCTGGac                                                   >  2:843084/1‑238 (MQ=255)
                                                                                                                                                                                cgcgGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCAGTGTCAGATCAGACTATCGACCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGCCCGCTTCTGGAGATGCTGGaa                                                   >  2:1127323/1‑239 (MQ=255)
                                                                                                                                                                                cgcgGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATACTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGaa                                                   >  1:614063/1‑239 (MQ=255)
                                                                                                                                                                                             ttttttgCATCTTTTTTTTTATTACTCTCACACAAAACATTTTACTTTTTGTTTCTTTTTTGTTTATGTTTTTTTATCCTGCCAAAAAAAATAAAATAATCATTTTTTCCCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGata                                      <  1:48059/237‑1 (MQ=255)
                                                                                                                                                                                                   gaacttttttttttATAACATACAATAAAAACATATTGCTTTTTTTTTCTTTTTTTTTGATGTTTTTTTCTCTTCCTTAAAAAGATTAAAAAATCATCTTTTCCCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAAt                                <  2:83358/230‑1 (MQ=255)
                                                                                                                                                                                                                    aCATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGa                                                                                     >  1:907410/1‑169 (MQ=255)
                                                                                                                                                                                                                      aTTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGCTTATAGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGt             >  2:380641/1‑239 (MQ=255)
                                                                                                                                                                                                                        tCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGCTGATGTTTTATCATGCCTGAAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCTCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGTAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGACTCAACCTCCCTGAACAATGTgg           >  2:1086398/1‑239 (MQ=255)
                                                                                                                                                                                                                        ccaataaaaaCATTTTACGTTTTTTTTCTTTTTTTTTTATTATTTTTTATCTGGCCTAAAAAAATAAAAAATCCAGCATTCCCCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTgg           <  1:1069397/233‑1 (MQ=255)
                                                                                                                                                                                                                          aCTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGAt         >  1:1087785/1‑239 (MQ=255)
                                                                                                                                                                                                                            tCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGa                                                                                     >  1:236428/1‑161 (MQ=255)
                                                                                                                                                                                                                                 ctttatTCCGTTTTGTCTCATTTTTGTTGAGTATGTTATAACATCTCAAAAAAAATGAAAAAATTAGCATTCCCCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAg  <  2:861179/236‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                   
GTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAG  >  NC_000913/1877393‑1877856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: