| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,877,629 | C→T | 20.9% | intergenic (‑97/‑86) | yoaF ← / → yeaP | DUF333 family outer membrane lipoprotein/diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,877,629 | 0 | C | T | 20.9% | 87.6 / 5.3 | 37 | intergenic (‑97/‑86) | yoaF/yeaP | DUF333 family outer membrane lipoprotein/diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base C (23/6); new base T (2/6); total (25/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.71e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAG > NC_000913/1877393‑1877856 | gTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCt < 1:453873/238‑1 (MQ=255) aaTAGACCGACGCCGGATTTGCCATACCAATCTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTtgatg < 1:78911/238‑1 (MQ=255) gACGCCGGATTTGCCATACCAATCTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTTACTCAAAACATATTACGTTTTGTTTTATCTTTGTTGATGATGtttttt > 1:507760/1‑237 (MQ=255) tttGCCATACCAATCTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGc < 1:779109/239‑1 (MQ=255) tGCCATACCAATCTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCaa < 1:686325/239‑1 (MQ=255) tGCCATACCAATCGGCGGCGGGTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCATGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCaa < 1:373248/239‑1 (MQ=255) aTCTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCGGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAaata > 1:821236/1‑239 (MQ=255) cTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAaataat < 1:280809/239‑1 (MQ=255) cggcggTTTAGGTGGTTCTGGCTGAGAAGGGGTTGAACAAACAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATTGTTTTTTTTTTCAGCACTATTTTTTTTATATTCACTCAAAACATTTTACGTTTTTTTTTATCTTTTTTGTTTATGTTTTATTATGCCTTCAAAGATTAAATAATCAg > 2:48059/1‑239 (MQ=255) ggTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTAccc > 2:1069397/1‑238 (MQ=255) gAACAACCAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACTCGCCGTATCCTGGAGTTGTTCAgtt > 2:491761/1‑237 (MQ=255) acCAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGtcaga > 1:1033996/1‑238 (MQ=255) ccagccagGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGtcagatc > 1:1193719/1‑239 (MQ=255) gcaagcaaGGCAGAACAAAACTGATACTTTTCATTTATTGATCTCACATATTTATCCCAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTTAATAATCAGCATTTACCCGCCGTATCCTGAAGTTGATCCGTGTCCGCTCAGATTATCGccc > 2:661955/1‑238 (MQ=255) caagcaagGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTAAATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATTCCTGCAAAGATTAAATAATCAGCGTTTACCCGCCGTATCCTGGAGTTGTTTCTTTTCAGATCAAATTTTCGCCcgc > 2:3798/1‑239 (MQ=255) ggCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGc > 1:928201/1‑239 (MQ=255) aaaatataatatTTTTATTTTTTTCATCTCACATATTAATCAAATATTAGAGTACCGGTTTTTTTTTTTTTTTTGCATTATTTTTTTTATAATTTTCAATAAAAATATATTACTTTTTTTTTCTTTTTTGTTGATTGTTTTTTATCCTGCATGAAAAAATAAAAAAATCAGTTTTTCCCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCt < 2:507760/231‑1 (MQ=255) aTAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCg > 1:1133990/1‑223 (MQ=255) attttttttttttgacaccatttatTTATCAAAGATTAGAGTATCGCTGTATGTTTTTTTTTGGCAGTTTTTTTTTTATTATTTTCACAAAAAATATATAACTTTTTTATTCTTTTTTTTTTTTGTTTTTTTATCAGGCTTGAAATAATTAAATAATCACATTTTCCCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAAc < 2:543415/217‑1 (MQ=255) gATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAATTAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCTCCCGCGTCTCGCAATCTCTTTCCCAAGAACAGTCACTGGCAAGTCTGGTCCGACCGCTTCTg > 2:1182522/1‑238 (MQ=255) gATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTgg > 1:83358/1‑239 (MQ=255) gATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTgg > 1:484585/1‑239 (MQ=255) tCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGa > 1:236405/1‑239 (MQ=255) tCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGa > 1:694857/1‑239 (MQ=255) tCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAAATACTGGa > 1:897040/1‑239 (MQ=255) cgcgGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAACTCTGGTCCGACAGCTTCTGGCGATGCTGGac > 2:843084/1‑238 (MQ=255) cgcgGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCAGTGTCAGATCAGACTATCGACCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGCCCGCTTCTGGAGATGCTGGaa > 2:1127323/1‑239 (MQ=255) cgcgGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATACTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGaa > 1:614063/1‑239 (MQ=255) ttttttgCATCTTTTTTTTTATTACTCTCACACAAAACATTTTACTTTTTGTTTCTTTTTTGTTTATGTTTTTTTATCCTGCCAAAAAAAATAAAATAATCATTTTTTCCCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGata < 1:48059/237‑1 (MQ=255) gaacttttttttttATAACATACAATAAAAACATATTGCTTTTTTTTTCTTTTTTTTTGATGTTTTTTTCTCTTCCTTAAAAAGATTAAAAAATCATCTTTTCCCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAAt < 2:83358/230‑1 (MQ=255) aCATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGa > 1:907410/1‑169 (MQ=255) aTTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGCTTATAGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGt > 2:380641/1‑239 (MQ=255) tCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGCTGATGTTTTATCATGCCTGAAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCTCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGTAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGACTCAACCTCCCTGAACAATGTgg > 2:1086398/1‑239 (MQ=255) ccaataaaaaCATTTTACGTTTTTTTTCTTTTTTTTTTATTATTTTTTATCTGGCCTAAAAAAATAAAAAATCCAGCATTCCCCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTgg < 1:1069397/233‑1 (MQ=255) aCTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGAt > 1:1087785/1‑239 (MQ=255) tCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGa > 1:236428/1‑161 (MQ=255) ctttatTCCGTTTTGTCTCATTTTTGTTGAGTATGTTATAACATCTCAAAAAAAATGAAAAAATTAGCATTCCCCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAg < 2:861179/236‑1 (MQ=255) | GTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGCGGTTTAGGTGCTTCTGGCTGAGAAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAGGCAGAACAAAACTGATAATTTTCATTTATTGATCTCACATATTTATCCAAGATTAGAGTATCGCGGTATCGTTTTGTTTTGCAGCACTATTTTTATTACATTCACTCAAAACATATTACGTCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAG > NC_000913/1877393‑1877856 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |