Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,826,408 G→A 14.6% E7K (GAA→AAA)  srlE → glucitol/sorbitol‑specific enzyme IIB component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,826,4080GA14.6% 225.3 / 7.0 85E7K (GAA→AAA) srlEglucitol/sorbitol‑specific enzyme IIB component of PTS
Reads supporting (aligned to +/- strand):  ref base G (56/16);  new base A (2/11);  total (58/27)
Fisher's exact test for biased strand distribution p-value = 3.06e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATCAACCCTGGCGAACTGTTTGTTTATCTT‑GGCATTGCC‑AGCGGTCTGACAA‑CGCTGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGC‑TGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTAT  >  NC_000913/2826177‑2826643
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               
tatcacaccgtcctacttgtttgttTATCTT‑GGCTTTGCC‑AGTGGTTCGCCAC‑GGCTGACCCTCCCCCTTGGCCCTCTGGCGGTGAGTTATCTGC‑TGTTTGGTCTGGTCACCAATTTCTACCGCGGCTGGGTGACCGATCTAACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑gg                                                                                                                                                                                                                                         <  2:465292/222‑1 (MQ=255)
    caCCCTGGGCACCTTTTTGTTTATGTT‑GCGTTTGCC‑GGCGTTCTGACAC‑CGCTAGACCGGCCACTTGGCCCACTGGCGGTGAGTAATCTGC‑TGGTTGGTCTGGTCACAAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACg                                                                                                                                                                                                                                     <  2:402803/238‑1 (MQ=255)
     aCCCTGGCGAACTGTTTGTTTATCTT‑GGCATTGCC‑AGCGGTCTGACAA‑CGCTGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTTC‑TGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACgg                                                                                                                                                                                                                                    >  1:414101/1‑239 (MQ=255)
     aCCCTGGCGAACTGTTTGTTTATCTT‑GGCATTGCC‑AGCGGTCTGACAA‑CGCTGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGC‑TGGTTGGGCTGGTCACCAATTTCTTCCCCGGGTGGGTGACCCATCTGGCCACCGC‑CCGTTTTG‑AGAAAAAGATGGGCAT‑TCAACATGAGCAAAAA‑GATCCCCTGGCAGGGGCAACAACATGACGCATATTCGGATCAAAAAA‑GGAACgg                                                                                                                                                                                                                                    >  2:928065/1‑239 (MQ=255)
     aCCCTGGCGAACTGTTTGTTTATCTT‑GGCATTGCC‑AGCGGTCTGACAA‑CGCTGAACCTGCCACTTGGCCCACTGGCGGTGAGGTATCTGC‑TGGTTGGTCTGGTCACCAATTTCTTCCCCGGCTGGGTGACCCATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAAAATCATGACGCATATTCGGATCGAAAAA‑AGAACgg                                                                                                                                                                                                                                    >  1:1167577/1‑239 (MQ=255)
          ggCGAACTGTTTGTTTATCTT‑GGCATTGCC‑AGCGGTCTGACAA‑CGCTGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGC‑TGGTTGGTCTGGTCCCCAATTTCTTCCGCGGCTGGGTGACCTATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTTAACAAAAA‑GTTCACCCGGCAGGAGCGACCTAAAGACGCTTTTTCCGGTCGAAAAA‑GGAACGGGGGGc                                                                                                                                                                                                                               >  1:636819/1‑239 (MQ=255)
          ggCGAACTGTTTGTTTATCTT‑GGCATTGCC‑AGCGGTCTGACAA‑CGCTGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGC‑TGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGC‑CATTTTTG‑AGATAAAGATGGGCAT‑TCAACTTGAAAAAAAA‑GGTCACCTGGCAGGAGCAACATCATGAAGCAGATTCTGATCGAAAAA‑GAAAAggggaca                                                                                                                                                                                                                               >  2:148165/1‑235 (MQ=255)
          ggCGAACTGTTTGTTTATCTT‑GGCATTGCC‑AGCGGTCTGACAA‑CGCTGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGC‑TGGTTGGGCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGGTCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGGAT‑TCAACTTGAACAAAAA‑GTTCCCCTGGCAGGGGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACggggg                                                                                                                                                                                                                                 >  1:794735/1‑235 (MQ=255)
           gCGAACTGTTTGTTTATCTT‑GGCATTGCC‑AGCGGTCTGACAA‑CGCTGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGC‑TGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGC‑CCTTTTTG‑AGAAAAAGAGGGGCAT‑TCAAATTTGACAAAAA‑GTTCACCTGGCAGGAGACACCACATTACGCATATTCGGAACGAAAAA‑AGGAAGGGGGGcg                                                                                                                                                                                                                              >  2:822734/1‑238 (MQ=255)
           gCGAACTGTTTGTTTATCTT‑GGCATTGCC‑AGCGGTCTGACAA‑CGCTGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGC‑TGGTTGGGCTGGTCACCAATTTTTTCCGCGGCTGGGTGACCGATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATTGGCAT‑TCAACTTGGACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGAACGGAAAA‑GGAACGGGTg                                                                                                                                                                                                                                 >  1:1028429/1‑236 (MQ=255)
               ctgttttgtttTTCTT‑GGC‑TTGCCTGGGGGTCT‑ACAACCGCTGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGC‑TGGTGGGTCTGGTCCCAAATTTCTTCCGCGGCGGGGTGACCGATTTGCCCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTgggg                                                                                                                                                                                                                          <  2:1139201/235‑1 (MQ=255)
                   ttttttttTCTTGGGCTTTGCC‑GGCGGCTTGACCA‑GGGTGACCCGCCCACTTGGCCCACTGGCGTGGAGTTATCTGC‑GGGTTGGCCTGGCCACCAATTTCTTCCGCGGCTGGGGGCCCGATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGgg‑cg‑g                                                                                                                                                                                                                     <  2:1060664/239‑1 (MQ=255)
                   tttgtttATCTT‑GGCATTGCC‑AGCGGTCTGACAA‑CGCTGAACCTGCCACTTGGCCCACTGGCGGTGAGATTTCTTC‑TGGTTTGGCTGGGTCCCAAATTTTTCCGCGGCTGGGGGGCCGGACTGGGCACCTG‑CATTTTTG‑AGAAAAAGAATGGGAT‑TCAACTTGAACAAAAA‑CATCACCTCGCAGGAGCAACAACAAGACACATATTTGGATCGAAAAA‑AGAAAGGGTGGCTgggtggg                                                                                                                                                                                                                       >  2:579943/1‑234 (MQ=255)
                   tttgtttATCTG‑GGCATTGCC‑CGCGGTCTGAAAC‑CGCTGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGC‑TGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGgg‑cg‑g                                                                                                                                                                                                                     <  1:1108317/238‑1 (MQ=255)
                           tCTT‑GGCATTGCC‑AGCGGTCTGACAA‑CGCTGAACCTGCCACTTGGCCCACTGGCGGGGGGGTATCTGC‑CGGTTGGGCTGGTCCCCCATTTCTTCCCCGGCTGGGTGACCCATCTGACCACCGC‑CATTTTTG‑AGAAAAAGGTGGGGCT‑TCAACTTGGACAAAAA‑GGTCACCCTGCCGGAGCAACATCCTGACGCATATTCGGATCAAAAAA‑GGAACCGGGGGGCGGGG‑CG‑GCCCGCtcta                                                                                                                                                                                                            >  2:1052320/1‑236 (MQ=255)
                             tt‑GGCATTGCC‑AGCGGTCTGACAA‑CGCTGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGC‑TGGTTGGTCTGGTCACCCATTTCTTCCGCGGCTGGGTGACCGAGCTGTCCACCGC‑CCTTTTTG‑AGAAAAAGATGGGGAT‑TCAACTTGAACAAAAA‑GGTCACCTGGCAGGAGCAAAATCATGACGCATATTCGGGTCGAAAAA‑GGAACGGGGGGCTGGGG‑CG‑GCCCGCCTGAg                                                                                                                                                                                                           >  2:1108317/1‑238 (MQ=255)
                                      gCC‑AGCGGTCTGACAA‑CGCTGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGC‑TGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCCCCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGGGGc                                                                                                                                                                                                                               >  1:838468/1‑212 (MQ=255)
                                      gCC‑AGCGGTCTGACAA‑CGCTGAACCTGCCACTTGGCCCACTGGCGGGGTGTTATCTGT‑TGGGTGGTCTGGTCACCCATTTCTTCCGCGGCTGGGTGACCCATCTGGCCACCTC‑CCTTTTTC‑GGAAAAAAATGGCGAT‑TCAAACTTCACCAAAA‑GTTTACCTGGCAGGAGACACATCATGACCCATTTTAGGGACGAGAAA‑GGAACGGGTGGCCGGGG‑CG‑Gccccccttagcccaaaca                                                                                                                                                                                                   >  2:32086/1‑223 (MQ=255)
                                           acggtcttcaaatgtcTGAGCCTGCCCCTTGGCCCACTGGGGGTGAGTTAT‑TGCTTGTTTGGTCTGGTCCCCATTTTCTTCCGGGGCGGGGTGACCAATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACg                                                                                                                                                                                               <  2:1167577/224‑1 (MQ=255)
                                              gTCTGACAC‑CGCTGACCCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGC‑TGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCg                                                                                                                                                                                            <  1:781028/238‑1 (MQ=255)
                                               ttcgcacagcgtgtcctcTGCCCCTTGGCCCCCTGGGGGGGAGTTATCTGC‑GGGTGGGCCTGGTCACAAATTTCCTCCGGGGCTGGGTTCACAATGTGACAACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCg                                                                                                                                                                                            <  2:262034/221‑1 (MQ=255)
                                                      a‑CGCTAACCCTGCCCTTTGGCCCACTGGCGGTGAGTTATCTGC‑TGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGaaaaaaa                                                                                                                                                                                   <  1:399522/239‑1 (MQ=255)
                                                             accctcccctgtGGCCCACGGGCGGTGATTTATCTGC‑TGGTGGGTCTGGTCACCAATTTCTCCCGCGGCTGGGTGACCGATCTGCCCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTc                                                                                                                                                                            <  1:822734/228‑1 (MQ=255)
                                                              aCCGGCCACTGGGCCCGCTGGCGGTGAGTTATCTGC‑TGGTTGGTCTGGCCACCAATTTCTTCCGCGGCTGGGGGACCAATCTCACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCt                                                                                                                                                                           <  2:794735/239‑1 (MQ=255)
                                                                       ttGGCCCACTGGCGGTGAGTTATCTGC‑TGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCCGGAAAAAAAAT‑CGCCTATATcccc                                                                                                                                                                   >  1:585453/1‑238 (MQ=255)
                                                                         ggCCCACTGGCGGTGAGTTATCTGC‑TGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCCCCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAACCACGCCGGGAAAAAAAAA‑CGTCTATATCAccgc                                                                                                                                                                 >  1:540103/1‑238 (MQ=255)
                                                                              aCTGGCGGTGAGTTATCTGC‑TGGTTGGGCTGGTCACCAATTTCTTCCGCGGCTGGGGGACCGATCTGAGCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑ATTCACCTGGCAGGAGCAACATCATGACGCATATTCGGGTCGAAAAA‑AGAACGGGTGGCTGGGG‑CG‑GCCCGCGTGAGCTGAAAGCCCCGCCGGGAAAAAAAAA‑CGACTGTATCCCCCCCggga                                                                                                                                                            >  2:243741/1‑236 (MQ=255)
                                                                                            aTCTGC‑TGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCCCCGCCGGTACCCGGCCTGCGAttg                                                                                                                                             >  2:420132/1‑239 (MQ=255)
                                                                                            aTCTGC‑TGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGAttg                                                                                                                                             >  1:63948/1‑239 (MQ=255)
                                                                                                gc‑tgGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGAttgttg                                                                                                                                          >  1:1040320/1‑238 (MQ=255)
                                                                                                     gttggtCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACACAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACaa                                                                                                                                      >  2:1201238/1‑238 (MQ=255)
                                                                                                     gttggtCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGCTTTTTGACaa                                                                                                                                      >  2:830965/1‑238 (MQ=255)
                                                                                                     gttggtCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACaa                                                                                                                                      >  1:1152483/1‑238 (MQ=255)
                                                                                                     gttggtCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACaa                                                                                                                                      >  1:1405/1‑238 (MQ=255)
                                                                                                          tgtggtcACCTATTTCTCCCGCGGCTGGGTGACCGATCTGACCCCCGC‑CATTTTTG‑AAAAAAAGATGGGCAT‑TCAATTTGAAAAAAAA‑GTTCACCTGGCGGGAGCAACATCATGCCGCATTTTCGGAACAAAAAA‑GGAAGGGGTGGCTGGGG‑CG‑CCCCGTTGGAGCTGAAAGCCACGCGGGAAAAAAAAAC‑CGTTTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTgg                                                                                                                                 <  1:441470/236‑1 (MQ=255)
                                                                                                              gtcACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGt                                                                                                                                                                             >  1:1016889/1‑190 (MQ=255)
                                                                                                              gtcACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGcaca                                                                                                                             >  1:393953/1‑238 (MQ=255)
                                                                                                               tcACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGcaca                                                                                                                             >  1:1011316/1‑237 (MQ=255)
                                                                                                               tcACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGcaca                                                                                                                             >  1:852836/1‑237 (MQ=255)
                                                                                                                           tcCGCGGCTGGGTGACCGATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGTGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCCCAGCTTActggctgg                                                                                                                >  1:625062/1‑238 (MQ=255)
                                                                                                                               cgGCTGGGTGACCGATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGc                                                                                                                                                      >  1:277687/1‑196 (MQ=255)
                                                                                                                                      cagcagcactgcacccccGC‑CATTTTGG‑A‑AAAAAAAGGGGGATATCAACTGAAACAAAAA‑GTCCACGTGGGGGGAGCAACATCATGACGTATGTCTGGA‑CAAAAAA‑AGAAGCGGTGGCCGGGGCCG‑GCGCGCTGGCCCTAAACCCCTCG‑CGGAAAAAAAAATCCCTTTAACTCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGa                                                                                                     <  2:250220/226‑1 (MQ=255)
                                                                                                                                       tgccctatttgccccccGC‑CTTTTTTA‑AAAAAAAGATGGCCAT‑TCAATTTAAAAAAAAA‑TTCCACCTGGCGGGAGCAACATCATGCCGCATATCGGGATCAAAAAA‑GGACCGGGGGGCGGGGG‑CG‑GCCCGTTTGAGCTAAAGGCCACGCGGGAAAAAAAAAC‑CGTCTATACCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGAc                                                                                                    <  1:703507/223‑1 (MQ=255)
                                                                                                                                       tcagctgtcaacaactcctcatttttgG‑AAAAAAAGGGGGGTAT‑ACAATTGAAAAAAAAA‑GTACCCTGGGCGGGAGCACAACCAGCGCGCATAGTGGGACCAAAAAA‑GGAAGGGGTGGCTGGGG‑CG‑GCCCGCTTGAGGTAAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCGCCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGa                                                                                                     <  2:943856/216‑1 (MQ=255)
                                                                                                                                        gaccggatctacccccctccttgtttG‑AAAAAAAGAGGGGAAT‑CCAATTTAAAAAAAAA‑GTCAACTGGGCAGGGGCAACATCATGCCGAATATTCGGACAGAAAAA‑GGAACGGGTGGCGGGGG‑CG‑CCCCGCTGGGGCTGAACGCCCCGCCGGGAAAAAAAAC‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGAc                                                                                                    <  2:501244/215‑1 (MQ=255)
                                                                                                                                         ccgaatttgccccccGC‑CATTTGTG‑AAAAAAAGATGGGATT‑TCAACTTGAAAAAAAA‑GTTCCCCTGGCAGGAGCAACAACATGACGCATATCCGGACAAAAAAA‑GGAACGGGGGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGAAAAAAAAAT‑CGTCTATACCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGa                                                                                                 <  1:346806/226‑1 (MQ=255)
                                                                                                                                               cTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTaaa                                                                                           >  1:529741/1‑239 (MQ=255)
                                                                                                                                                ataccaccGCGCATTTTTGAAGAAAAAGA‑GGGGAA‑TCAAAATGAAAAAAAAGGTACGGCGGGGGGGAGCAAAATCAAGACGCATA‑TGGGAACAAAAAAGGGAAGGGGTGGCTGGGC‑CG‑GCCCGTTGGGTCTAAACCCCACGGCGGAAAAAAAAAT‑CGTCTAAATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTa                                                                                             <  2:540103/236‑1 (MQ=255)
                                                                                                                                                      ccGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTg                                                                                    >  1:976422/1‑239 (MQ=255)
                                                                                                                                                       cGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGa                                                                                   >  2:850516/1‑239 (MQ=255)
                                                                                                                                                       cGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACACCGGGAAAAAAAAT‑CGTCTATATCACAGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTAACTGGCTGGAAGGCTATTGGAGGAGTTAAAACAGGTGa                                                                                   >  2:654836/1‑239 (MQ=255)
                                                                                                                                                       cGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGCGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATACCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGCTTTAAAGAAGGtta                                                                                   >  2:1067661/1‑237 (MQ=255)
                                                                                                                                                                  g‑agaAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGTATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGCAAGCCACGCCGGGAAGAAAAAT‑CGTCTATATCACGGCCGGTACCCGGCCTTCGAGTGTTGACAAACTGGCCCAGCTTACTGGCTGGCAGGCTATTCACGGACTTAACGAAGGTGAACACGCggcg                                                                         >  2:635738/1‑237 (MQ=255)
                                                                                                                                                                             tGGGCAT‑ACAACTTGAAAAAAAA‑GTTCACGTGGGAGAGGAAACATCATGACACATATTCGGATCGAAAAA‑GGAGCGGGTGGCTGGGG‑CG‑GCCCGTTTGAGCGGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATACCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAAt                                                                 <  1:974003/237‑1 (MQ=255)
                                                                                                                                                                                    tccAACTAGAAAAAAAA‑AGCCACCGGTCGGGAGCAACAGCATGACGCATATGCGAATCGAAAAA‑GGAGCGGGGAGC‑GGGG‑CGCCCCCGCTTGAGCGGAACCCCCCGGCGGAAAAAAAAAC‑CGTCTATACCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTcg                                                         <  2:1011316/237‑1 (MQ=255)
                                                                                                                                                                                            gAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATc                                                 >  1:141358/1‑239 (MQ=255)
                                                                                                                                                                                            gAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATc                                                 >  1:399968/1‑239 (MQ=255)
                                                                                                                                                                                                  aaa‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACtgt                                           >  1:1134198/1‑239 (MQ=255)
                                                                                                                                                                                                   aa‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACtgtg                                          >  2:324376/1‑239 (MQ=255)
                                                                                                                                                                                                        ccccggggagggggCAACATCATGACGCATATGGGGAACAAAAAA‑GGACGGGGTGGCGGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGAAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTggcg                                       <  1:243741/225‑1 (MQ=255)
                                                                                                                                                                                                         gacggggCAGGAGCACCATGAACGCGCATTTTCGTAAAGAAAAA‑GGAGCGGGGGGGTGGGG‑CG‑GCCCGCTGGCGCGAAAACCCACGCCGAAAAAAAAAAC‑GCTCAATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTggcg                                       <  2:596551/232‑1 (MQ=255)
                                                                                                                                                                                                               gCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGAGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACAtt                                >  1:275770/1‑238 (MQ=255)
                                                                                                                                                                                                                cAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTAc                              >  1:609189/1‑239 (MQ=255)
                                                                                                                                                                                                                 aGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGGGGAAATTGGGGTCGCGGTAATCGGATGTggggggcaagtag                              >  2:1080635/1‑230 (MQ=255)
                                                                                                                                                                                                                  gtaGCACCGTCATGCGGCATATCGGGACGGAAAAG‑GAAAGGGGGGGCGGGGC‑CG‑GCCCGCTGGAGCGGAACGCCCCGCCGGTAAAAAAAAC‑CGTCAATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACg                             <  2:414101/236‑1 (MQ=255)
                                                                                                                                                                                                                      aaaCATCAGGACGAAATTTCGGATCAAAAAA‑GGAAGGGGTGGCGGGGG‑GG‑CCCCGTTGGAGCTGAAAGCAACGCGGAGAAAAAAAAC‑CGTCTATACCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCg                        <  1:928065/238‑1 (MQ=255)
                                                                                                                                                                                                                       aaCATCATGACGAATATTCGGATCAAAAAA‑GGAACGGGGGGCGGGGG‑CG‑GCCCGTTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATACCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCgg                       <  1:767265/239‑1 (MQ=255)
                                                                                                                                                                                                                         catcatGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACg                             >  1:596551/1‑231 (MQ=255)
                                                                                                                                                                                                                            aatGAAGAATATGCGGAACAAAAAA‑GGAACGGGGGGCGGGGG‑CG‑CCCCGTTTGAGCTGAAAGCCCCGCCGGAAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTcg                                                         <  2:98140/199‑1 (MQ=255)
                                                                                                                                                                                                                              tGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTa                 >  1:528845/1‑238 (MQ=255)
                                                                                                                                                                                                                                  gCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGa            >  1:1146797/1‑239 (MQ=255)
                                                                                                                                                                                                                                   cATATTAGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGaa           >  2:366025/1‑239 (MQ=255)
                                                                                                                                                                                                                                    atatTGGGAACAAAAAG‑GGAGCGGGGGGCTGGGG‑GG‑CCCCCCTTAGTCTGAAGGCCACGGCGGAAAAAAAAAT‑CGTCTATACCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGaa           <  1:350817/238‑1 (MQ=255)
                                                                                                                                                                                                                                    atatTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGaaa          >  1:110326/1‑239 (MQ=255)
                                                                                                                                                                                                                                    atatTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGaa           >  1:1006596/1‑238 (MQ=255)
                                                                                                                                                                                                                                     tatTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAac         >  1:1050576/1‑239 (MQ=255)
                                                                                                                                                                                                                                      atTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGa                                               >  1:98140/1‑200 (MQ=255)
                                                                                                                                                                                                                                      atTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAacg        >  1:836629/1‑239 (MQ=255)
                                                                                                                                                                                                                                           gATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTTCGGGATCTATCCGAAacggcggt   >  2:24793/1‑237 (MQ=255)
                                                                                                                                                                                                                                           gATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGAGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTTTGGCGGCACAGTACGCTGCGGCATCTATCCGAAacgacgca   >  1:1015409/1‑237 (MQ=255)
                                                                                                                                                                                                                                            aTCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACCTTACGCCGCGGCATCTATCCGAAACGACGTAt  >  1:942510/1‑239 (MQ=255)
                                                                                                                                                                                                                                            aTCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTAt  >  1:977855/1‑239 (MQ=255)
                                                                                                                                                                                                                                            aTCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGGGTCGCGGTAATCGACTGTGGCGGCCCATTACGCTGCGGTCTCTATCCGAAACGACGTAt  >  2:391477/1‑239 (MQ=255)
                                                                                                                                                                                                                                            aTCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGGCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCCGGCTATTGACGGATTTAAAGAAGGCGGACCCGCGGAGGAGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCCCATTACGCTGCGGCATCTATTCGCAacgaactat  >  2:858085/1‑234 (MQ=255)
                                                                                                                                                                                                                                            aTCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCAAGCCGGGAAAAAAAAT‑CTTCTATATCACCGCCGGTACCCGGCCTGTGATTGTAGACAAACTTGCACAGCTTACTGGCTGGAAGGCTAATGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGGGTCGCGGGAATCGTCTGTGTCGTCAAGATTGGCTTGGGGGTCTATCCGGAACGTCGTAt  >  2:890717/1‑239 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               
TATCAACCCTGGCGAACTGTTTGTTTATCTT‑GGCATTGCC‑AGCGGTCTGACAA‑CGCTGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGC‑TGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGC‑CATTTTTG‑AGAAAAAGATGGGCAT‑TCAACTTGAACAAAAA‑GTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAA‑GGAACGGGTGGCTGGGG‑CG‑GCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAAT‑CGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTAT  >  NC_000913/2826177‑2826643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑