Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,206,485	C→A	20.9%	Q9K (CAG→AAG)	ttdA →	L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,485	0	C	A	20.9%	159.7 / 6.5	77	Q9K (CAG→AAG)	ttdA	L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (10/47);  new base A (14/3);  total (26/51)
Fisher's exact test for biased strand distribution p-value = 1.75e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑CCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCC  >  NC_000913/3206253‑3206723                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  aTCGTTAATTATCCGCATTTGTGATAAGCGCAGTGTATTTCGCAAAACATCGCCATTTCCGTCTCTGGCGTAATATTCATTCTTATTTTAGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑CCAGACAAATAC‑ATAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGc                                                                                                                                                                                                                                               <  2:418246/237‑1 (MQ=255) aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑CCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAgg                                                                                                                                                                                                                                                <  1:466107/236‑1 (MQ=255) aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑CCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAgg                                                                                                                                                                                                                                                <  2:679385/236‑1 (MQ=255) aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑CCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAgg                                                                                                                                                                                                                                                <  1:245308/236‑1 (MQ=255) aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑CCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGc                                                                                                                                                                                                                                               <  1:405568/237‑1 (MQ=255) aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑CCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGc                                                                                                                                                                                                                                               <  1:527421/237‑1 (MQ=255) aTCGTTAATTATCCGAAGTTGCGATAAGCGCAGTGTATTTCGCAAAACATTGTCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑CCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAgg                                                                                                                                                                                                                                                <  2:884321/236‑1 (MQ=255)      tAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCTATATTCAGTAGTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTG‑‑CCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGaa                                                                                                                                                                                                                                         <  2:362577/238‑1 (MQ=255)         tgctCCGCAGTTCGGATACGCGCAGTGTATTTCTCAAAACATTGCCATTACCTGCTTTAGCGCAATATTCATTCTTATGTTCATGTTGATTTACGAAC‑TCTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGGTTGTGGCGG‑TGAA‑TTTTT‑‑CCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGTCACTGAATAAg                                                                                                                                                                                                                                     <  2:165387/236‑1 (MQ=255)              cGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑ATTTT‑‑TTATAAAAATAA‑AAAAAAT‑G‑GA‑GGTGTCA‑TGGTGAGGG‑GTAGTAATAAACAACAAGCAATGAACAAGTGGAc                                                                                                                                                                                                                                >  2:42500/1‑239 (MQ=255)               gCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TATTT‑‑TCAGACAAATAC‑AAAAAAT‑G‑GG‑GTTTCCA‑GGGTTGGCG‑AAAGATAAAAGCAAAACGCAGTGAATAAATTGGCa                                                                                                                                                                                                                               >  1:766827/1‑239 (MQ=255)                    tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑CCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                                                                                                                                                                           <  2:560479/238‑1 (MQ=255)                    tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑CCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                                                                                                                                                                           <  1:849748/238‑1 (MQ=255)                    tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑CCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                                                                                                                                                                                          <  1:74784/239‑1 (MQ=255)                    tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑CCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                                                                                                                                                                                          <  1:457775/239‑1 (MQ=255)                        aTAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGGA‑ATTTT‑‑TCACACAAAAAC‑AAAAAAT‑T‑GA‑GTTGCCA‑TGATGAGAG‑GAAATAAAAAAAAAAAAGAAATGAATAAGTTTAAAAAAATTGTc                                                                                                                                                                                                                      >  2:757226/1‑239 (MQ=255)                        aTAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑TTATACAAAAAA‑AAAAAAT‑G‑AC‑GTTGTCC‑TTAGCAGGGCAAAGT‑ATAAAAAAAAAGCAATGAAAAAGTGTACAGAGATTg                                                                                                                                                                                                                        >  2:13286/1‑237 (MQ=255)                           aGCTCAGTTTATTTTGAAAAACATTGCCATTCCCTGCTCTGTCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAT‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑AAAAAACCACAATTTTAGTGGTATTTTGTGGCGG‑TAAA‑TTTTT‑‑CAAAACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                                                                                                                                                                                   <  2:609553/239‑1 (MQ=255)                           aGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑CCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                                                                                                                                                                                   <  1:13286/239‑1 (MQ=255)                                  tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTGAT‑TTTTT‑‑CCAG‑CAAAAACAAAAAATT‑G‑AA‑ATTTCCA‑‑GATTGGGG‑GAAGTAATAAAGAACAAGCAAGGAAAAAATTGACAAAGAATTTTGCTAACattt                                                                                                                                                                                                            >  1:1045251/1‑238 (MQ=255)                                  tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTGAA‑TTTTT‑‑‑AAAAAAAAAAC‑AAAAAAT‑GTGA‑TTTGCCAGAGATGAGAG‑‑AGGTAA‑AATACATAAGAAGATAATAAGCTGTCAGAGATTCTTGCTAACttt                                                                                                                                                                                                             >  2:1124421/1‑238 (MQ=255)                                  tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCTGAA‑TTTTTCACCAAACAAAT‑C‑‑‑AAACT‑G‑GA‑GTTGCCA‑AGATGAGCG‑GAAAGAATAAACTAAACGCAATGAAAAAGTTCTCagaacatgttccacatat                                                                                                                                                                                                              >  1:950472/1‑222 (MQ=255)                                        tcccAAAACTTCGCTATTCCCGGCTTTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATTTCCGCGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTATTTTGGGGGGG‑TGAA‑TTTTC‑‑CCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCACCAGGCAGTGAAAAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCa                                                                                                                                                                                                     <  1:909580/236‑1 (MQ=255)                                          acAAAACATTGCCATTCCCTGCTCTGGCGAAATATTAATTCTTATTTTCTCTTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑AAAAAACCACATTTTTAGTGGTAGTTTGGGGCGG‑TGAA‑TTTTT‑‑CCAGACAAATAC‑AAAAACG‑G‑GA‑TTTGCCA‑TGATGAGCG‑AAAGTAATAACCAACAGGCAGTGAATAAGTTGAAAGAGATTGTCGCTACTTTTAC‑CGCCATg                                                                                                                                                                                                   <  1:391680/238‑1 (MQ=255)                                            aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑TCACACAAAAAA‑TAAAAAT‑T‑GA‑GTTTTGC‑TTATTAGCG‑TAAATAATAAAAAATACGAAGAGAATAAATTAGAACAGAGTGTCGCTCACCTTAT‑ACCCATGAt                                                                                                                                                                                                 >  1:854016/1‑239 (MQ=255)                                              aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTT‑‑CAAGACAAA‑AC‑AAAAAAT‑A‑CA‑GTTGCCA‑TCATGGGCG‑GAAGGAATAAGACACAAGCAGTGACAAAGTTGACAAAGATTATTGATAACTTTtc‑ctcccttcttt                                                                                                                                                                                               >  2:915883/1‑232 (MQ=255)                                                   tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑TCAGACAAATAC‑AAAAAAT‑G‑TA‑GGTGCCA‑TGATTAGCG‑AAAGTAATAAAAAACAGGGAGGGAATAAGTTTACAGAGAGTGTGGCTAAATTTAC‑CCCCATGATTTCT‑Ac                                                                                                                                                                                          >  1:1101461/1‑238 (MQ=255)                                                      taTTCCCTTGTTTGCCGAAATATTCATTCTTATTTTCGCTTTGATTCACGAAA‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑AAAAAACCAAATTTTTAGTGGTAGTTTGGGGCGG‑TGAA‑TTTTC‑‑CCAAACAAATAA‑AAAAACT‑G‑GA‑GTCGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGa                                                                                                                                                                                      <  1:847011/238‑1 (MQ=255)                                                       aTTCCCTTCTCGGGCGAAATATTCATTCTTATTTTCGCTTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑AAAAAACCACATTTTTAGTGGTAGTTTGTGGGGG‑TGAA‑TTTTT‑‑CCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGaa                                                                                                                                                                                     <  1:829109/239‑1 (MQ=255)                                                          cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑CCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGc                                                                                                                                                                                                                                               <  1:1021953/180‑1 (MQ=255)                                                           cctgctgtgacgtatttgtcagtcTTATTTACACGTGGATTCACAAAC‑TTTTAATTTGATATTGACGGTGGAAAAAAAA‑ATAAAACCCCATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTC‑‑CAAGCAAAAACC‑AAAAACT‑G‑GT‑TTGGCCA‑GGTTAAGCA‑AAACTAAAAGGCAAAAGGCCGTGAAAAAGTTAAAATTGATGTTCGGTACCTTTTC‑CGCCATGATTTCT‑ACCCGAATGcc                                                                                                                                                                                 <  2:768641/221‑1 (MQ=255)                                                                aaaaagcaaaTATTCATTCTAATTTCGGCGTTAATTC‑CAAACTTGTTAATTACAATTTGAGGGTGGAAAAAAAT‑AAAAAACAAAATTTTAAGTGGTAGTTGGTGGGGG‑TGAA‑TTTTT‑‑CAAAAAAAAAAA‑AAAAACG‑G‑GA‑GTGGCCA‑TGAGGAGCG‑AAAGTAATAAGCAACAGGTAGTGAATAAGTTGACGGAGATTGTCGTTACCTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCtgatg                                                                                                                                                                            <  2:339379/231‑1 (MQ=255)                                                                          tataCATTCTTATTTTCGCGTTAATTCACGACC‑TGTTAATTACATATTCACGGTGGAAAAAAAT‑ATAAAACCCCATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTA‑‑CCAGAAAAATAC‑AAAAACT‑G‑GA‑GTTGCAA‑TGAGGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAAAAGTTGACAGAGATTGTCGCTAATTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                  <  1:166732/239‑1 (MQ=255)                                                                          aattcactcTTATTTTCGGTTTGATACACAAAG‑TGTTAATTACATATGCACGGTGGAAAAAAAT‑AAAAAACAAAATTTTGAGGGGTAGTTTGTGGGGT‑AGAA‑TTTTT‑‑CCAGAAAAAAAA‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCA‑AAAGTAATAAGCAACGGGCAATAAATAAGTTGACAGATATTGTCGCTAATTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                  <  2:125645/238‑1 (MQ=255)                                                                                 tcTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑ATTTT‑‑TCCGACAAAAAC‑AAAAACA‑A‑GA‑GATGGCC‑TGATGAGCG‑GAAATAAAAAGAAAAAAGCAGTGAAAAAGATGACACAGAATTTAGCACAATTTAT‑CCCCCTGATTTCT‑ACCCGAATGTCtcatggagggggggatttgtt                                                                                                                                                            >  1:339379/1‑222 (MQ=255)                                                                                  cTTATTTTCGTGTTTATTCACGAAC‑TGTAAATTAAATATTCACGGTGAGAAAAAAA‑ATAAAACCAAATTTTGAGGGGGAGTTTGTGGCGG‑TGAA‑TTTTT‑‑CAAGAAAAAAAC‑AAAAACT‑G‑GT‑GTTGCCA‑TGATGAGCG‑AAATTAATAAGCAACAGGGAGTGAATAAGTTGAAAGAGATTGTCGATAACTTAAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTaa                                                                                                                                                          <  1:608541/239‑1 (MQ=255)                                                                                  cTTATTTACGGATTGATCCATGATC‑TGTTAATTAAATATTAAGGGTGAAAAAAAAT‑AAAAAACCACATTTTAAGTGGAATTTAGTGGCGG‑TGAA‑TTTTA‑‑CCAGAAAAAAAA‑AAAACCG‑G‑GA‑TTTGCAA‑TGATGAGCG‑AAAGTAATAACCAACAGGTAGTTAAAAAGTGGAAAGAGATTGTCGTTACTTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTaa                                                                                                                                                          <  2:529126/239‑1 (MQ=255)                                                                                  cTTATTTACGCTTTAATTCACGAAT‑TGTTAATTACATATGCACGGTGGCAAAAAAT‑AAAAAACCACATTTTTAGGGGTAGTTTGTGGCGG‑TAAA‑TTTTC‑‑CCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TAATGAGCG‑AAAGTAAAAAGCAACAGGCAGTGAATAATTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTaa                                                                                                                                                          <  1:148751/239‑1 (MQ=255)                                                                                   ttATTTTCGTGTTAATCCACAAAC‑TGTTAATTACATTTTCACGGTGGCAAAAAAT‑AAAAAACAACATTTATAGGGGTGGTTTGTGGCGG‑TAAA‑TTTTC‑‑CAAAAAAAATAC‑AAAAACG‑G‑GA‑GTTCCAA‑TAAGGATCA‑AAATAAATAAGCAACAGGCATTAAATAAGTGGAAAGAGATTGTCGTTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                                                                         <  2:161297/239‑1 (MQ=255)                                                                                    tATTTTCGCGTTGATTCACGAAC‑TTTTAATTACATATCCCCGGGGGAAAAAAAT‑AAAAACCCACATTTTTAGTGGTAGTTTGTGGCGG‑TAAA‑TTTTT‑‑CAAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCA‑AAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                        <  1:42500/239‑1 (MQ=255)                                                                                    tATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑ATTTT‑‑TTATACAAAAAC‑AAAAACT‑T‑GA‑GTTGCCA‑TTGTGTGGG‑TAACGAAAAAAAAAAAAGCACAGAAAAAATTTGAAAAGATTTTGCATAACTTTTA‑CGCCCATATTTCT‑ACCCGAATGCCTCGTGAAGCGGTGGGTAAACTaaa                                                                                                                                                         >  2:702996/1‑238 (MQ=255)                                                                                    tATTTTCGCGTTGATTCAAGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑AAAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑CAAGACAAATAC‑AAAAACG‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                        <  1:154220/239‑1 (MQ=255)                                                                                     aagaagtgtataacggaaataagtGTTAATTAGATATTCACGGGGGAAAAAAAT‑AAAAAACCAAATTTTTAGGGGTATTTTGTGGGGG‑TGAA‑TTTAT‑‑CAAGAAAAATAA‑AAAAACT‑G‑GA‑GTTGCCA‑GGATGAGGA‑AAATTAATAAACAACAGGAAGTGAATAAGTGGACAGAGATTGGCGCTAATTTAAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                        <  2:120312/216‑1 (MQ=255)                                                                                                             gTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑ATTTT‑‑TCAAACAAAAAC‑AAAAAAA‑G‑GA‑GTTTGCA‑TTGTGGGGG‑AAAGGAAAAAAAAAAAAGGAAGGAAAAAATTGACAGAGATTTTCGCTAACCTTAA‑CCCCAAGATTTTT‑TACCGAATGACTGGAGACGGGGGGGGTAAAAAAAAAAAACTTAAAGAACCCGaaaaggcc                                                                                                                                >  2:25389/1‑234 (MQ=255)                                                                                                                 attaattatTCAGGGGGAAAAAAAAT‑ATAAAACAACATTTATAGTGGTAGTTTGTGGGGG‑TAAA‑TTTTA‑‑CCAGACAAATAC‑AAAAACG‑G‑AA‑GTTGCCA‑TGATGAGGA‑AAAGTAATAAGCAACAGGCAGTTAATAAGTTGACTGAGATGGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGa                                                                                                                           <  1:344051/233‑1 (MQ=255)                                                                                                                  ttAAATATCCAC‑GTTGCAAAAAATAATAAAACCACTGTATTAGTGGTAGTTTGTGTCGG‑TGAA‑TTATG‑‑CCAGAAAAATAC‑AAAAACG‑G‑GA‑GTTTCAA‑GGATGAGCA‑AAATTAATAAGCAACAGGAAGGTAATAGGTTGACAGAGATTGTCGATATTTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGAt                                                                                                                          <  2:322415/239‑1 (MQ=255)                                                                                                                    aCATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑TCAAACAAAATA‑AAAAAAT‑G‑GA‑GTTGCCA‑TGGTGTGGG‑GAAAGAATAAACAAAAAGAAAGGGAAAAGGTTACAAAAAATTGCGCTAAATTTTA‑CGCCCAGATTTTT‑TCCCGAAAGCCTGATTGCGTGGGGGGTAAAATAAAAAAGCTAAAAGaatgtcttttttacaacact                                                                                                                          >  1:120312/1‑219 (MQ=255)                                                                                                                       tgttcggggTGGCAAAAAAT‑AAAAAACCAAATTGTTAGTGGTAGTTTGTGGGGG‑TGAA‑TTTTC‑‑CCAAAAAAAAAC‑AAAAACT‑G‑GT‑GTTGCCA‑GAATGAGGA‑AAATTAAAAAGCAACGGGCAGTGAATAGGTGGACAGAGATTGTCGTTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGa                                                                                                                     <  2:1045251/232‑1 (MQ=255)                                                                                                                       tatTCAGGGTGGAAAAAAAT‑AAAAAACCACATTTTTAGTGGTAGTTTGGGGCGG‑TAAT‑TTTTT‑‑CCAGACAATTAA‑AAAAACA‑G‑GA‑GTGGCCA‑TGAGGAGGA‑AAAGTAAAAAGAAACAGGCGGGGAAGAAATGTAAATAGGTGGCCGGAAGCTTCAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGa                                                                                                                     <  1:109395/239‑1 (MQ=255)                                                                                                                        atTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑TCAAACAAAAAA‑AAAAAAT‑T‑GA‑GTTTCCA‑TGATGAGCG‑GAAAGAATAAACAAAAAGCAGTGAAAAAGATTACAGAGATTGTCGCTACATTTAC‑CCCCATGATTTCT‑ACCCGAAAGCCTGATGACGTGGTGGAGAAACTAAAAAAACTAAAAGAAGCCGGAACATCG‑TCGATggggga                                                                                                                    >  1:958632/1‑237 (MQ=255)                                                                                                                        atTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑CCAGACAAATAC‑AAAAAAT‑T‑GA‑GTTGCCA‑TGATGAGCG‑CAAGTAATAAACAACAAGCAGGGGAAAAAATGACAGAGAGTTTCGCTAAATTTAC‑CGCCATGATTTTT‑ACCCGAATACCTGATGAAGGGGGGGGTAAACTAAAAAAACTAAAGGATGCCGAAAACACG‑TCGGTGGGGaa                                                                                                                    >  1:1101168/1‑239 (MQ=255)                                                                                                                        atTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑ATTTT‑‑TCTGACAAATAA‑AAAAAAT‑G‑GG‑GGTGTCA‑TTGAGGGCG‑GAAGGTAAAAACAAAAAGAAAAGAATAAATTTATAGAGAACGTCGCTAACTTTACACCCCAGATTTTTT‑ACCCAAATG‑CTGAGGCAGGGGTGGGTAAAATAAAAAAAATTAAAGATATCCAAAAGTCG‑TCGAtatggga                                                                                                                    >  2:726296/1‑233 (MQ=255)                                                                                                                        atTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑ATTTT‑‑TCAAACAAAAAC‑AAAAAAT‑G‑GA‑GTTGGCA‑TGGTGAGCG‑AAAGTAATAAACAAAAAGCAGAGAAAAAAATGATAGAGATTGGCTGTAAATTTAC‑CACCACGATTTTT‑ACCCGAAGACCAGATGACGCGGGGGATAAACAAAAAAAAACAAAAAATTCCGACAcgtcg‑tacaggggggg                                                                                                                    >  2:966271/1‑229 (MQ=255)                                                                                                                            aCGGTGGAAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTGGTGGCGG‑TGAA‑TTTTC‑‑CCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCA‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAAtt                                                                                                                <  1:757226/239‑1 (MQ=255)                                                                                                                            aCGGTGGAAAAAAAT‑AAAAACCCAAATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑CAAAAAAAATAA‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCA‑AAAGTAATAAGAAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAAtt                                                                                                                <  1:470818/239‑1 (MQ=255)                                                                                                                              ggtgaaaaaaaaT‑AAAAAACCACATTTTTAGTGGTAGTTGGGGGCGG‑TGAA‑TTTTC‑‑CCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTAt                                                                                                              <  1:25389/233‑1 (MQ=255)                                                                                                                               ggggCAAAAAAT‑ATAAAACCATATTTTTGGGGGTAGGTTGGGGCGG‑TGAA‑TTTTT‑‑CCAGACAAATAC‑AAAAACG‑G‑GA‑GTTGCCA‑TGAGGAGGG‑AAAGTAATACGCAAAGGGTAGTGAATAGGTTGACAGAGATTGTCGTTACCTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTAt                                                                                                              <  1:164518/236‑1 (MQ=255)                                                                                                                                tGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑TCAAACAAAAAA‑AAAAAAT‑T‑GAGGTTGCCT‑TGGTGGGGG‑AAATAAAAAAAAAACA‑GCAAAGAATAAGGTGAACAAGATTTTCCCTTAATTTAA‑CACCAATATTTTTAACCCGATGCCCTGTTACGGTGTGGGAAAAACAAAAAAAGTCAAAGAAACCCAAAACGCCG‑GCG‑TGGGGGAAATTAttt                                                                                                            >  2:285026/1‑237 (MQ=255)                                                                                                                                tGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑CGAA‑TTTTT‑‑TTAGACAAAAAA‑AAAAACT‑G‑GA‑GGTGTCA‑TGGTGGGGG‑AAAGTAATAAGCAAAAAGAAAAGAAAAAATTGACAGAGATTGTCGCGAAATTTTC‑AACCACAATTTTT‑TCACGAATACCTGGTGTCGGGGGGGGTACACTCAAAAAACTAAAAAATGCCGAAAcgtcgctgagtgggtaaaaaaatc                                                                                                             >  2:382661/1‑220 (MQ=255)                                                                                                                                      aaaaT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑CCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑GGATGAGCA‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCAAATTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATa                                                                                                      <  1:1124421/239‑1 (MQ=255)                                                                                                                                       tgaataagaaaaaacaTTTTTAGTGGGAGTTTGTGGGGG‑TAAA‑TTTTC‑‑CCAAAAAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑GGATGAGCA‑AAAGTAAAAAGCAACGGGCAGTGAATAAGTTGACAGAGATTGTCGCTACCTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATa                                                                                                      <  2:814272/226‑1 (MQ=255)                                                                                                                                        aaT‑ATAAAACCACATTTTTGGTGGTAGTTGGGGGGGG‑TGAA‑TTTTT‑‑CAAAAAAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGAGGAGCA‑AAAGTAATAAGCAACAGGCAGGGAATAAGTTGAAAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACg                                                                                                    <  1:915883/239‑1 (MQ=255)                                                                                                                                               aaaCCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑ATTTT‑‑TCAAACAAAAAA‑AAAAAAA‑T‑GA‑GGTGGCA‑TGATGGGGG‑AAAGGAATAAAAAACAAGCAAAAAAAAAAATGAAAGAGAATGTCGCCAACTTTAC‑ACCCATGATTTCT‑ACCCCAAAAACTGATGACGGGGGGGATAAACTAAAAAAGTCAAACAGAGCCGCAACATCG‑TCGGTGGGGGAAATTATATTCCCCACGAAGTTc                                                                                              >  2:350200/1‑239 (MQ=255)                                                                                                                                                     caTTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑TCAGACAAATAC‑AAAAACT‑T‑GA‑GGTTTCA‑TGGTGCGAC‑ATAATAAAATACAAAAGACATTGAATGAGGTGTAAGAATTTCTAGCTTAAATTAC‑CGCCCTCAGTTTT‑ACCACCACGACTGATGACGTGGTGGATATACTCACACACCTTAATGCGGCCACAACATCGCTCGA‑GGGGGAAATTATAATCAATACCATGTTCTacaaa                                                                                        >  2:12608/1‑238 (MQ=255)                                                                                                                                                     caTTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑TCAGACAAAAAA‑AAAAAAT‑T‑GA‑GGTGGCA‑TGAAGAGCG‑AAAGGAATAAACAAAAAGCAAAGAATAAATTGACAAAGGATTTCGCTAAATTTTC‑CGCCATGATTTTT‑ACACGAAAGACCGATGACGGGGTGGGTAAAATAAAAAAACTAAAACAAAGCGAAACGGCG‑GCGCTGGGGGAAAATATCTAACATAcaaaggtcggaaaa                                                                                        >  2:842129/1‑226 (MQ=255)                                                                                                                                                               ggTAGTTTG‑GGGGG‑GGAA‑ATTTT‑‑TCAAAAAAAAAC‑AAAAACTGG‑GA‑TTTGCAA‑TGATGAGGA‑AAAGTAAAAAGAAACAGAAAGTGAATAGGTTAAAAGAGATTGCCGTTAATTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCaaaa                                                                                <  2:83476/237‑1 (MQ=255)                                                                                                                                                                   gtgtggggggg‑TAAA‑TTTTG‑‑CAAAAAAAAAAC‑AAAAAGT‑G‑AA‑GTTGCCA‑GGGTGAGCG‑AAAGTAATAAGCAAGAGGAAGTAAATAAGTGGAAGGAAATTGTCGTTAATTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                           <  2:353338/229‑1 (MQ=255)                                                                                                                                                                                        ttcccAGACAAATAA‑AAAAACT‑G‑GA‑GTTGCCA‑TGGTGAGCG‑AAATTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                         <  1:538631/236‑1 (MQ=255)                                                                                                                                                                                                  aaTAG‑AAAAACA‑G‑AA‑GTTGCAA‑TGAGGAGCG‑AAATTAATAAGCAACAGGAAGTGAATAAGTAGACAGAGATTGTCGATAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGa                                               <  1:884732/239‑1 (MQ=255)                                                                                                                                                                                                     aC‑AAAAACG‑G‑GA‑TTTGCCA‑TGATGAGCG‑AAATTAATAACCACCAGGAAGTAAATAATTTGACAGAGATTGTCTCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGacac                                            <  1:1054575/239‑1 (MQ=255)                                                                                                                                                                                                          aaaCT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg                                        >  2:55895/1‑239 (MQ=255)                                                                                                                                                                                                          aaaCT‑G‑GA‑GTGGCGA‑TGATGAGCG‑AAAGTAATAAGCAAAAGGCAGTGAAAAAGTGAACAGAGATTGACGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg                                        <  2:401234/239‑1 (MQ=255)                                                                                                                                                                                                           aaCT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAACCAACAGACAGTGAATAAGTTGACAGGTATTGTCTCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                       <  1:915853/239‑1 (MQ=255)                                                                                                                                                                                                           aaCT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAATAATAAGCAACAGGCAGGGAATAAGTTGACAGAGATTGTCCCTACCTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                       <  1:382661/239‑1 (MQ=255)                                                                                                                                                                                                               aggtagattgacatggatgaGGA‑AAAGAAAAAAGCAACAGGCATGAAATAATATGACGTAGTTTGCGTCTGTCTTAAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGGGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                       <  2:403372/224‑1 (MQ=255)                                                                                                                                                                                                                              gatgaGCG‑AAAGTAATAAGCAACAGGCAGTGAATACGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAACCTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGCTGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACTTGAATCGTCCTGCCTGTCAGGACACCGGAGAGATTATGTTCTTCg                        >  2:643429/1‑239 (MQ=255)                                                                                                                                                                                                                                    cG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg                  >  2:736753/1‑239 (MQ=255)                                                                                                                                                                                                                                                    ccAGGCAGTGAATAAGTTGACAGAGAGTGTCGCTATCTTTAC‑CGACATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTcc   <  1:458224/238‑1 (MQ=255)                                                                                                                                                                                                                                                     cAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACACCATGCAACAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGtt                             >  1:475701/1‑212 (MQ=255)                                                                                                                                                                                                                                                     cAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTccc  >  1:651527/1‑239 (MQ=255)                                                                                                                                                                                                                                                     cAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGCTCCCGCTTccc  >  1:340625/1‑239 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  ATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAC‑TGTTAATTACATATTCACGGTGGCAAAAAAT‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAA‑TTTTT‑‑CCAGACAAATAC‑AAAAACT‑G‑GA‑GTTGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAC‑CGCCATGATTTCT‑ACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCG‑TCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCC  >  NC_000913/3206253‑3206723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑