Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,504 C→A 23.1% T15K (ACA→AAA)  ttdA → L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,5040CA23.1% 118.1 / 9.5 75T15K (ACA→AAA) ttdAL‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (21/34);  new base A (6/12);  total (29/46)
Fisher's exact test for biased strand distribution p-value = 7.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTT‑‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCG  >  NC_000913/3206266‑3206734
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                         
cGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTT‑‑TTATAAAAATAAAAAAAATGGAGGTGTCATGGTGAGGGGTAGTAATAAACAACAAGCAATGAACAAGTGGAc                                                                                                                                                                                                                                           >  2:42500/1‑239 (MQ=255)
 gCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATATTT‑‑TCAGACAAATACAAAAAATGGGGTTTCCAGGGTTGGCGAAAGATAAAAGCAAAACGCAGTGAATAAATTGGCa                                                                                                                                                                                                                                          >  1:766827/1‑239 (MQ=255)
      tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTT‑‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                                                                                                                                                                                      <  2:560479/238‑1 (MQ=255)
      tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTT‑‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                                                                                                                                                                                      <  1:849748/238‑1 (MQ=255)
      tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTT‑‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                                                                                                                                                                                                     <  1:457775/239‑1 (MQ=255)
      tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTT‑‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                                                                                                                                                                                                     <  1:74784/239‑1 (MQ=255)
          aTAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTT‑‑TCACACAAAAACAAAAAATTGAGTTGCCATGATGAGAGGAAATAAAAAAAAAAAAGAAATGAATAAGTTTAAAAAAATTGTc                                                                                                                                                                                                                                 >  2:757226/1‑239 (MQ=255)
          aTAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTT‑‑TTATACAAAAAAAAAAAATGACGTTGTCCTTAGCAGGGCAAAGTATAAAAAAAAAGCAATGAAAAAGTGTACAGAGATTg                                                                                                                                                                                                                                   >  2:13286/1‑237 (MQ=255)
             aGCTCAGTTTATTTTGAAAAACATTGCCATTCCCTGCTCTGTCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAATTGTTAATTACATATTCACGGTGGCAAAAAATAAAAAACCACAATTTTAGTGGTATTTTGTGGCGGTAAATTTTT‑‑CAAAACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                                                                                                                                                                                              <  2:609553/239‑1 (MQ=255)
             aGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTT‑‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                                                                                                                                                                                              <  1:13286/239‑1 (MQ=255)
                    tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTGATTTTTT‑‑CCAGCAAAAACAAAAAATTGAAATTTCCAGATTGGGGGAAGTAATAAAGAACAAGCAAGGAAAAAATTGACAAAGAATTTTGCTAACattt                                                                                                                                                                                                                       >  1:1045251/1‑238 (MQ=255)
                    tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTGAATTTTT‑‑AAAAAAAAAACAAAAAATGTGATTTGCCAGAGATGAGAGAGGTAAAATACATAAGAAGATAATAAGCTGTCAGAGATTCTTGCTAACttt                                                                                                                                                                                                                        >  2:1124421/1‑238 (MQ=255)
                    tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCTGAATTTTTCACCAAACAAATC‑‑AAACTGGAGTTGCCAAGATGAGCGGAAAGAATAAACTAAACGCAATGAAAAAGTTCTCagaacatgttccacatat                                                                                                                                                                                                                         >  1:950472/1‑222 (MQ=255)
                          tcccAAAACTTCGCTATTCCCGGCTTTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATTTCCGCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTATTTTGGGGGGGTGAATTTTC‑‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCACCAGGCAGTGAAAAAGTTGACAGAGATTGTCGCTAACTTTACCGCCa                                                                                                                                                                                                                <  1:909580/236‑1 (MQ=255)
                            acAAAACATTGCCATTCCCTGCTCTGGCGAAATATTAATTCTTATTTTCTCTTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTT‑‑CCAGACAAATACAAAAACGGGATTTGCCATGATGAGCGAAAGTAATAACCAACAGGCAGTGAATAAGTTGAAAGAGATTGTCGCTACTTTTACCGCCATg                                                                                                                                                                                                              <  1:391680/238‑1 (MQ=255)
                              aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTT‑‑TCACACAAAAAATAAAAATTGAGTTTTGCTTATTAGCGTAAATAATAAAAAATACGAAGAGAATAAATTAGAACAGAGTGTCGCTCACCTTATACCCATGAt                                                                                                                                                                                                            >  1:854016/1‑239 (MQ=255)
                                aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTT‑‑CAAGACAAAACAAAAAATACAGTTGCCATCATGGGCGGAAGGAATAAGACACAAGCAGTGACAAAGTTGACAAAGATTATTGATAACTTTtcctcccttcttt                                                                                                                                                                                                          >  2:915883/1‑232 (MQ=255)
                                     tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTT‑‑TCAGACAAATACAAAAAATGTAGGTGCCATGATTAGCGAAAGTAATAAAAAACAGGGAGGGAATAAGTTTACAGAGAGTGTGGCTAAATTTACCCCCATGATTTCTAc                                                                                                                                                                                                     >  1:1101461/1‑238 (MQ=255)
                                        taTTCCCTTGTTTGCCGAAATATTCATTCTTATTTTCGCTTTGATTCACGAAATGTTAATTACATATTCACGGTGGCAAAAAATAAAAAACCAAATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTC‑‑CCAAACAAATAAAAAAACTGGAGTCGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa                                                                                                                                                                                                 <  1:847011/238‑1 (MQ=255)
                                         aTTCCCTTCTCGGGCGAAATATTCATTCTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGGGGTGAATTTTT‑‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa                                                                                                                                                                                                <  1:829109/239‑1 (MQ=255)
                                             cctgctgtgacgtatttgtcagtcTTATTTACACGTGGATTCACAAACTTTTAATTTGATATTGACGGTGGAAAAAAAAATAAAACCCCATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTC‑‑CAAGCAAAAACCAAAAACTGGTTTGGCCAGGTTAAGCAAAACTAAAAGGCAAAAGGCCGTGAAAAAGTTAAAATTGATGTTCGGTACCTTTTCCGCCATGATTTCTACCCGAATGcc                                                                                                                                                                                            <  2:768641/221‑1 (MQ=255)
                                                  aaaaagcaaaTATTCATTCTAATTTCGGCGTTAATTCCAAACTTGTTAATTACAATTTGAGGGTGGAAAAAAATAAAAAACAAAATTTTAAGTGGTAGTTGGTGGGGGTGAATTTTT‑‑CAAAAAAAAAAAAAAAACGGGAGTGGCCATGAGGAGCGAAAGTAATAAGCAACAGGTAGTGAATAAGTTGACGGAGATTGTCGTTACCTTTACCGCCATGATTTCTACCCGAATGCCtgatg                                                                                                                                                                                       <  2:339379/231‑1 (MQ=255)
                                                            tataCATTCTTATTTTCGCGTTAATTCACGACCTGTTAATTACATATTCACGGTGGAAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTA‑‑CCAGAAAAATACAAAAACTGGAGTTGCAATGAGGAGCGAAAGTAATAAGCAACAGGCAGTGAAAAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                             <  1:166732/239‑1 (MQ=255)
                                                            aattcactcTTATTTTCGGTTTGATACACAAAGTGTTAATTACATATGCACGGTGGAAAAAAATAAAAAACAAAATTTTGAGGGGTAGTTTGTGGGGTAGAATTTTT‑‑CCAGAAAAAAAAAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACGGGCAATAAATAAGTTGACAGATATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                             <  2:125645/238‑1 (MQ=255)
                                                                   tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTT‑‑TCCGACAAAAACAAAAACAAGAGATGGCCTGATGAGCGGAAATAAAAAGAAAAAAGCAGTGAAAAAGATGACACAGAATTTAGCACAATTTATCCCCCTGATTTCTACCCGAATGTCtcatggagggggggatttgtt                                                                                                                                                                       >  1:339379/1‑222 (MQ=255)
                                                                    cTTATTTTCGTGTTTATTCACGAACTGTAAATTAAATATTCACGGTGAGAAAAAAAATAAAACCAAATTTTGAGGGGGAGTTTGTGGCGGTGAATTTTT‑‑CAAGAAAAAAACAAAAACTGGTGTTGCCATGATGAGCGAAATTAATAAGCAACAGGGAGTGAATAAGTTGAAAGAGATTGTCGATAACTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa                                                                                                                                                                     <  1:608541/239‑1 (MQ=255)
                                                                    cTTATTTACGGATTGATCCATGATCTGTTAATTAAATATTAAGGGTGAAAAAAAATAAAAAACCACATTTTAAGTGGAATTTAGTGGCGGTGAATTTTA‑‑CCAGAAAAAAAAAAAACCGGGATTTGCAATGATGAGCGAAAGTAATAACCAACAGGTAGTTAAAAAGTGGAAAGAGATTGTCGTTACTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa                                                                                                                                                                     <  2:529126/239‑1 (MQ=255)
                                                                    cTTATTTACGCTTTAATTCACGAATTGTTAATTACATATGCACGGTGGCAAAAAATAAAAAACCACATTTTTAGGGGTAGTTTGTGGCGGTAAATTTTC‑‑CCAGACAAATACAAAAACTGGAGTTGCCATAATGAGCGAAAGTAAAAAGCAACAGGCAGTGAATAATTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa                                                                                                                                                                     <  1:148751/239‑1 (MQ=255)
                                                                     ttATTTTCGTGTTAATCCACAAACTGTTAATTACATTTTCACGGTGGCAAAAAATAAAAAACAACATTTATAGGGGTGGTTTGTGGCGGTAAATTTTC‑‑CAAAAAAAATACAAAAACGGGAGTTCCAATAAGGATCAAAATAAATAAGCAACAGGCATTAAATAAGTGGAAAGAGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                                                                                    <  2:161297/239‑1 (MQ=255)
                                                                      tATTTTCGCGTTGATTCACGAACTTTTAATTACATATCCCCGGGGGAAAAAAATAAAAACCCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTT‑‑CAAGACAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                                   <  1:42500/239‑1 (MQ=255)
                                                                      tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTT‑‑TTATACAAAAACAAAAACTTGAGTTGCCATTGTGTGGGTAACGAAAAAAAAAAAAGCACAGAAAAAATTTGAAAAGATTTTGCATAACTTTTACGCCCATATTTCTACCCGAATGCCTCGTGAAGCGGTGGGTAAACTaaa                                                                                                                                                                    >  2:702996/1‑238 (MQ=255)
                                                                      tATTTTCGCGTTGATTCAAGAACTGTTAATTACATATTCACGGTGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTT‑‑CAAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                                   <  1:154220/239‑1 (MQ=255)
                                                                       aagaagtgtataacggaaataagtGTTAATTAGATATTCACGGGGGAAAAAAATAAAAAACCAAATTTTTAGGGGTATTTTGTGGGGGTGAATTTAT‑‑CAAGAAAAATAAAAAAACTGGAGTTGCCAGGATGAGGAAAATTAATAAACAACAGGAAGTGAATAAGTGGACAGAGATTGGCGCTAATTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                                   <  2:120312/216‑1 (MQ=255)
                                                                                               gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTT‑‑TCAAACAAAAACAAAAAAAGGAGTTTGCATTGTGGGGGAAAGGAAAAAAAAAAAAGGAAGGAAAAAATTGACAGAGATTTTCGCTAACCTTAACCCCAAGATTTTTTACCGAATGACTGGAGACGGGGGGGGTAAAAAAAAAAAACTTAAAGAACCCGaaaaggcc                                                                                                                                           >  2:25389/1‑234 (MQ=255)
                                                                                                   attaattatTCAGGGGGAAAAAAAATATAAAACAACATTTATAGTGGTAGTTTGTGGGGGTAAATTTTA‑‑CCAGACAAATACAAAAACGGAAGTTGCCATGATGAGGAAAAGTAATAAGCAACAGGCAGTTAATAAGTTGACTGAGATGGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGa                                                                                                                                      <  1:344051/233‑1 (MQ=255)
                                                                                                    ttAAATATCCACGTTGCAAAAAATAATAAAACCACTGTATTAGTGGTAGTTTGTGTCGGTGAATTATG‑‑CCAGAAAAATACAAAAACGGGAGTTTCAAGGATGAGCAAAATTAATAAGCAACAGGAAGGTAATAGGTTGACAGAGATTGTCGATATTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAt                                                                                                                                     <  2:322415/239‑1 (MQ=255)
                                                                                                      aCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTT‑‑TCAAACAAAATAAAAAAATGGAGTTGCCATGGTGTGGGGAAAGAATAAACAAAAAGAAAGGGAAAAGGTTACAAAAAATTGCGCTAAATTTTACGCCCAGATTTTTTCCCGAAAGCCTGATTGCGTGGGGGGTAAAATAAAAAAGCTAAAAGaatgtcttttttacaacact                                                                                                                                     >  1:120312/1‑219 (MQ=255)
                                                                                                         tgttcggggTGGCAAAAAATAAAAAACCAAATTGTTAGTGGTAGTTTGTGGGGGTGAATTTTC‑‑CCAAAAAAAAACAAAAACTGGTGTTGCCAGAATGAGGAAAATTAAAAAGCAACGGGCAGTGAATAGGTGGACAGAGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                                <  2:1045251/232‑1 (MQ=255)
                                                                                                         tatTCAGGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTAATTTTTT‑‑CCAGACAATTAAAAAAACAGGAGTGGCCATGAGGAGGAAAAGTAAAAAGAAACAGGCGGGGAAGAAATGTAAATAGGTGGCCGGAAGCTTCACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                                <  1:109395/239‑1 (MQ=255)
                                                                                                          atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTT‑‑TCAAACAAAAAAAAAAAATTGAGTTTCCATGATGAGCGGAAAGAATAAACAAAAAGCAGTGAAAAAGATTACAGAGATTGTCGCTACATTTACCCCCATGATTTCTACCCGAAAGCCTGATGACGTGGTGGAGAAACTAAAAAAACTAAAAGAAGCCGGAACATCGTCGATggggga                                                                                                                               >  1:958632/1‑237 (MQ=255)
                                                                                                          atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTT‑‑CCAGACAAATACAAAAAATTGAGTTGCCATGATGAGCGCAAGTAATAAACAACAAGCAGGGGAAAAAATGACAGAGAGTTTCGCTAAATTTACCGCCATGATTTTTACCCGAATACCTGATGAAGGGGGGGGTAAACTAAAAAAACTAAAGGATGCCGAAAACACGTCGGTGGGGaa                                                                                                                               >  1:1101168/1‑239 (MQ=255)
                                                                                                          atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTT‑‑TCTGACAAATAAAAAAAATGGGGGTGTCATTGAGGGCGGAAGGTAAAAACAAAAAGAAAAGAATAAATTTATAGAGAACGTCGCTAACTTTACACCCCAGATTTTTTACCCAAATGCTGAGGCAGGGGTGGGTAAAATAAAAAAAATTAAAGATATCCAAAAGTCGTCGAtatggga                                                                                                                               >  2:726296/1‑233 (MQ=255)
                                                                                                          atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTT‑‑TCAAACAAAAACAAAAAATGGAGTTGGCATGGTGAGCGAAAGTAATAAACAAAAAGCAGAGAAAAAAATGATAGAGATTGGCTGTAAATTTACCACCACGATTTTTACCCGAAGACCAGATGACGCGGGGGATAAACAAAAAAAAACAAAAAATTCCGACAcgtcgtacaggggggg                                                                                                                               >  2:966271/1‑229 (MQ=255)
                                                                                                              aCGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTGGTGGCGGTGAATTTTC‑‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAAtt                                                                                                                           <  1:757226/239‑1 (MQ=255)
                                                                                                              aCGGTGGAAAAAAATAAAAACCCAAATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTT‑‑CAAAAAAAATAAAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGAAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAAtt                                                                                                                           <  1:470818/239‑1 (MQ=255)
                                                                                                                ggtgaaaaaaaaTAAAAAACCACATTTTTAGTGGTAGTTGGGGGCGGTGAATTTTC‑‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                                         <  1:25389/233‑1 (MQ=255)
                                                                                                                 ggggCAAAAAATATAAAACCATATTTTTGGGGGTAGGTTGGGGCGGTGAATTTTT‑‑CCAGACAAATACAAAAACGGGAGTTGCCATGAGGAGGGAAAGTAATACGCAAAGGGTAGTGAATAGGTTGACAGAGATTGTCGTTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                                         <  1:164518/236‑1 (MQ=255)
                                                                                                                  tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTT‑‑TCAAACAAAAAAAAAAAATTGAGGTTGCCTTGGTGGGGGAAATAAAAAAAAAACAGCAAAGAATAAGGTGAACAAGATTTTCCCTTAATTTAACACCAATATTTTTAACCCGATGCCCTGTTACGGTGTGGGAAAAACAAAAAAAGTCAAAGAAACCCAAAACGCCGGCGTGGGGGAAATTAttt                                                                                                                       >  2:285026/1‑237 (MQ=255)
                                                                                                                  tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAATTTTT‑‑TTAGACAAAAAAAAAAACTGGAGGTGTCATGGTGGGGGAAAGTAATAAGCAAAAAGAAAAGAAAAAATTGACAGAGATTGTCGCGAAATTTTCAACCACAATTTTTTCACGAATACCTGGTGTCGGGGGGGGTACACTCAAAAAACTAAAAAATGCCGAAAcgtcgctgagtgggtaaaaaaatc                                                                                                                        >  2:382661/1‑220 (MQ=255)
                                                                                                                        aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTT‑‑CCAGACAAATACAAAAACTGGAGTTGCCAGGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCAAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATa                                                                                                                 <  1:1124421/239‑1 (MQ=255)
                                                                                                                         tgaataagaaaaaacaTTTTTAGTGGGAGTTTGTGGGGGTAAATTTTC‑‑CCAAAAAAATACAAAAACTGGAGTTGCCAGGATGAGCAAAAGTAAAAAGCAACGGGCAGTGAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATa                                                                                                                 <  2:814272/226‑1 (MQ=255)
                                                                                                                          aaTATAAAACCACATTTTTGGTGGTAGTTGGGGGGGGTGAATTTTT‑‑CAAAAAAAATACAAAAACTGGAGTTGCCATGAGGAGCAAAAGTAATAAGCAACAGGCAGGGAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                                               <  1:915883/239‑1 (MQ=255)
                                                                                                                                 aaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTT‑‑TCAAACAAAAAAAAAAAAATGAGGTGGCATGATGGGGGAAAGGAATAAAAAACAAGCAAAAAAAAAAATGAAAGAGAATGTCGCCAACTTTACACCCATGATTTCTACCCCAAAAACTGATGACGGGGGGGATAAACTAAAAAAGTCAAACAGAGCCGCAACATCGTCGGTGGGGGAAATTATATTCCCCACGAAGTTc                                                                                                         >  2:350200/1‑239 (MQ=255)
                                                                                                                                       caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTT‑‑TCAGACAAATACAAAAACTTGAGGTTTCATGGTGCGACATAATAAAATACAAAAGACATTGAATGAGGTGTAAGAATTTCTAGCTTAAATTACCGCCCTCAGTTTTACCACCACGACTGATGACGTGGTGGATATACTCACACACCTTAATGCGGCCACAACATCGCTCGAGGGGGAAATTATAATCAATACCATGTTCTacaaa                                                                                                   >  2:12608/1‑238 (MQ=255)
                                                                                                                                       caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTT‑‑TCAGACAAAAAAAAAAAATTGAGGTGGCATGAAGAGCGAAAGGAATAAACAAAAAGCAAAGAATAAATTGACAAAGGATTTCGCTAAATTTTCCGCCATGATTTTTACACGAAAGACCGATGACGGGGTGGGTAAAATAAAAAAACTAAAACAAAGCGAAACGGCGGCGCTGGGGGAAAATATCTAACATAcaaaggtcggaaaa                                                                                                   >  2:842129/1‑226 (MQ=255)
                                                                                                                                                 ggTAGTTTGGGGGGGGAAATTTT‑‑TCAAAAAAAAACAAAAACTGGGATTTGCAATGATGAGGAAAAGTAAAAAGAAACAGAAAGTGAATAGGTTAAAAGAGATTGCCGTTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCaaaa                                                                                           <  2:83476/237‑1 (MQ=255)
                                                                                                                                                     gtgtgggggggTAAATTTTG‑‑CAAAAAAAAAACAAAAAGTGAAGTTGCCAGGGTGAGCGAAAGTAATAAGCAAGAGGAAGTAAATAAGTGGAAGGAAATTGTCGTTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                                      <  2:353338/229‑1 (MQ=255)
                                                                                                                                                                          ttcccAGACAAATAAAAAAACTGGAGTTGCCATGGTGAGCGAAATTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                                    <  1:538631/236‑1 (MQ=255)
                                                                                                                                                                                    aaTAGAAAAACAGAAGTTGCAATGAGGAGCGAAATTAATAAGCAACAGGAAGTGAATAAGTAGACAGAGATTGTCGATAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGa                                                          <  1:884732/239‑1 (MQ=255)
                                                                                                                                                                                       aCAAAAACGGGATTTGCCATGATGAGCGAAATTAATAACCACCAGGAAGTAAATAATTTGACAGAGATTGTCTCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGacac                                                       <  1:1054575/239‑1 (MQ=255)
                                                                                                                                                                                            aaaCTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg                                                   >  2:55895/1‑239 (MQ=255)
                                                                                                                                                                                            aaaCTGGAGTGGCGATGATGAGCGAAAGTAATAAGCAAAAGGCAGTGAAAAAGTGAACAGAGATTGACGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg                                                   <  2:401234/239‑1 (MQ=255)
                                                                                                                                                                                             aaCTGGAGTTGCCATGATGAGCGAAAGTAATAACCAACAGACAGTGAATAAGTTGACAGGTATTGTCTCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                  <  1:915853/239‑1 (MQ=255)
                                                                                                                                                                                             aaCTGGAGTTGCCATGATGAGCGAAAATAATAAGCAACAGGCAGGGAATAAGTTGACAGAGATTGTCCCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                  <  1:382661/239‑1 (MQ=255)
                                                                                                                                                                                                 aggtagattgacatggatgaGGAAAAGAAAAAAGCAACAGGCATGAAATAATATGACGTAGTTTGCGTCTGTCTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGGGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                  <  2:403372/224‑1 (MQ=255)
                                                                                                                                                                                                                gatgaGCGAAAGTAATAAGCAACAGGCAGTGAATACGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAACCTAAAACAGCTAAAGGATGCCGAAACGTCGTCGCTGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACTTGAATCGTCCTGCCTGTCAGGACACCGGAGAGATTATGTTCTTCg                                   >  2:643429/1‑239 (MQ=255)
                                                                                                                                                                                                                      cGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg                             >  2:736753/1‑239 (MQ=255)
                                                                                                                                                                                                                                      ccAGGCAGTGAATAAGTTGACAGAGAGTGTCGCTATCTTTACCGACATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTcc              <  1:458224/238‑1 (MQ=255)
                                                                                                                                                                                                                                       cacgCAGTCAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGCGGCGGATAAACTAAAACAGCTAAAGGGTGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATCGGATGTTCGACAACACGCAAAAAGCGATTGACCTGAATCACCCTGCCTGTCAGGACACAGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGATTacc             >  2:738786/4‑239 (MQ=255)
                                                                                                                                                                                                                                       cAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACACCATGCAACAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGtt                                        >  1:475701/1‑212 (MQ=255)
                                                                                                                                                                                                                                       cAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTccc             >  1:651527/1‑239 (MQ=255)
                                                                                                                                                                                                                                       cAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGCTCCCGCTTccc             >  1:340625/1‑239 (MQ=255)
                                                                                                                                                                                                                                        aGGTAGTAAATAAGTAGACAGATCTGGTCGATAACTATACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACATCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGTCCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCa            <  2:1201146/239‑1 (MQ=255)
                                                                                                                                                                                                                                        aGGCAGTGAATAAGTTGAAAGGGATGGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCa            <  1:871326/239‑1 (MQ=255)
                                                                                                                                                                                                                                                  tAATTTGAAAGATATTTTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCg  <  1:726296/239‑1 (MQ=255)
                                                                                                                                                                                                                                                  aaaGTGGAAAGAGTTGGTCGTTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCg  <  1:12608/238‑1 (MQ=255)
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                         
CGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTT‑‑CCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCG  >  NC_000913/3206266‑3206734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: