Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,499,692 G→T 19.9% intergenic (+79/‑216) yhfL → / → frlA small lipoprotein/putative fructoselysine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,499,6920GT19.9% 199.3 / 6.3 77intergenic (+79/‑216)yhfL/frlAsmall lipoprotein/putative fructoselysine transporter
Reads supporting (aligned to +/- strand):  ref base G (22/37);  new base T (14/2);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 4.66e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAA  >  NC_000913/3499467‑3499928
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   
gCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTACTGCACAGTAAATCTCTTACAGAAATCCGGTAACCCCGGTTTTATTGACCCACCCCCCCTTGGTTTTTCTAGGGGGTATTTTGTTCTTTTTTCTTTTTTccc                                                                                                                                                                                                                                     >  1:858327/1‑238 (MQ=255)
           tGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCTACTGCACAGGAAATTTCTGACCCAAATAGGCTAACACCCGTTTTATTTCCCCCAGCCCCCCTGGTTTTTCGGGGGGGGATTTTTTTTCTTGTCCTTCTTTCcccccccccctaaa                                                                                                                                                                                                                        >  1:513069/1‑233 (MQ=255)
              aGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGATGCCGTCCCACTCCGCCGTAAATCTCTTACAGAAATCGGCTTACACCCGGTGTATTTCCCGACCCCCTCTGGATTTTTCGAGGGCGCATTTGTTTTTATTTCCCTCTTTCCCCCCCCCCTGAACata                                                                                                                                                                                                                     >  2:676735/1‑239 (MQ=255)
                tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTGGGGCCCAATGACCACTAAGGCTCTGACCAAAAACTGATCACACCGGGTTTATTGTCCGACGCCCTTTGGGATTTTACAGGGCGGACTTTGTTTTATTTCGTTCTTTCCCCCCCCCTGAAAAATAtt                                                                                                                                                                                                                  >  1:1081316/1‑239 (MQ=255)
                tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCTCCTGCACCCCAAATTTTCTACACAAAACGGCTAACACCCGTTTTATTTTCCTCCCCCCTCCGGTTTTTTGCACGGCGGACTTGTTTCTTTGTTCTTCTTTTCCCCCCCCCTACAAataat                                                                                                                                                                                                                   >  2:704276/1‑237 (MQ=255)
                tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCCACTGCACAGTAAGTCTCTGAAAGAAATCGGCTAACACCCGTTTAATTTCCCGACCCCCTCTGGGATTTTCCGGGGCGTATTTTTTTCTATTTTCTTCTTTTCCCCCCCCGTCAAAATAtt                                                                                                                                                                                                                  >  1:1102114/1‑239 (MQ=255)
                tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCCGGCCCCCCGCCCCGTATATTTCTGACAAAAATACGCGCACTCCGGGTTTTTTTTCCGCACCCCCCTGGATTTTTCGTAGGAGTGATTGTTTTTGTATGCCTTTTTGCCCCCCCCCTTAAAAattt                                                                                                                                                                                                                    >  2:598568/1‑237 (MQ=255)
                tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGGAAATCTCTGAAAGAAAACGGCTAACACCCGGTTTATTTTCCGACGCCCCCCTGATTTTCCGAGGGCGGATTTGTTTCTATGTCCTTCTTTCCCCCCCCCGCCAAAAAAtt                                                                                                                                                                                                                  >  1:460045/1‑239 (MQ=255)
                 aCTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCTACCTCACAATAAATCTCTTGCAGAAATAGGCTAAAACCCGTTTATTTTTCCGACCCCCCCTGGATTTTCTCCGGGGGCCTTTTTTTCTATTTCTCTCTTTTCCCCCCCCCCGAAAAAattt                                                                                                                                                                                                                    >  1:512750/1‑237 (MQ=255)
                     gCTCCGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACCGTAAGTCTCTGACAGAAATCGGCTCACCCCGGTTTACTTTCCCGACCCCCCCTTGGTTTTTCCAGGGCGGCTTTTTTTCTATGTCCTTCTTTCCCCCGGCCCGTAAAATtttttt                                                                                                                                                                                                                >  1:430131/1‑237 (MQ=255)
                     gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTAATGCACAGTAAATCTCTGACAGAAATCGGCTAAAACCGGTTTTATTGCCCGCCGCCCTCTTGATTTTCCGAGGGCGTATTTGTTTCTTTGTTCTTTTTGCCCCCCTCCGGTAAAATAttttt                                                                                                                                                                                                               >  1:518172/1‑236 (MQ=255)
                        aCGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACttt                                                                                                                                                                                                          <  1:907741/239‑1 (MQ=255)
                          gTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGGTGCGGTCCCTCCGCCAAATAAATTTCTTACAACAATCGGGTAACACCCGGTTTATTTCCACGAGCCCTCTGGGCTTTCTGAGGGGGCCTTTTTTTCTTTTTCCCTTTTTCCCCCCCCCCTTAAAAAAtttttttttttt                                                                                                                                                                                                        >  2:407453/1‑231 (MQ=255)
                              aCTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTACTGCCCACTAAGTCTCTGACACAAATTGGCCAACACCCGTTTTATTTTCCGACCCCCCCTGGGGTTTTCCAGGGGGGTTTTTTTTTTTTTTTCTTCTTTCTCCCCCCCCTCAAAAATCTTTTcctttttattt                                                                                                                                                                                                     >  2:60123/1‑229 (MQ=255)
                                   aTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTACTGCACAGTAAATCTCTGACAGAAATTGGCTAACACCGGGTTAATTGCCCGCCGCCCCCTGGATTTTCCGAGGGCGTTTTTGTTTCTATTTCCTTCTTGCCCCCGCCCCTAAAAATATTTTTACTTTTAATTCAAttt                                                                                                                                                                                               >  1:1037896/1‑239 (MQ=255)
                                    tGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCTACTGCACCATAAGTCTCTGACACAAATAGCGTAACACCCGTTTATTTTCCCGCCGCCCTCTCGATTTTCCTGGGGCGCATATTTTTCTATTTCCCTCTTCCCCCCGCCCCTCAAAAGATTTTCACtttttttttaatttt                                                                                                                                                                                              >  2:546850/1‑227 (MQ=255)
                                    tGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCTACTGCACACTAAATCTCTGAAAGAAATCGGCTAACACCCGGTTAATTGCCCGCCCCCCCCCTGCTTTTTCCTGGGCGGATTTTTTTTTTTTTCCTTCTTGCCCCCCCCCCGTAAAATATATTTACtttttattttaattg                                                                                                                                                                                              >  2:1103166/1‑227 (MQ=255)
                                          ggTCATCTTGCAAACCGTGATAAGAACTGCACTTACGCCTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCACTGCCGGTTGTGGGACTTAGGCACCCAAAATCTTTAACAAGAATCGGGCTACAGCGGGTTCATTACTCGATCCCCTCTTGATGTTTCTCGCGAGGAGTTGTTTCCTCTGCCTTCTTGCCCCCCCCCGTCAAACAATTTTTTTTTTTTATTCGAGTTGATTACt                                                                                                                                                                                        >  2:777647/1‑239 (MQ=255)
                                          ggTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACCGAAAGTCTTTGAAAGAAAAACGCCAACACCGGGTTAATTTCCCGCCGCCCCCTGGGTTTTCCTAGGGCGGATTTGTTTCTATGTCCTTCTTGTCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTTATaaat                                                                                                                                                                                        >  1:139137/1‑237 (MQ=255)
                                           gTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCCCCTCCACAGTAAATTTTTGAAAAAAATTGGCGTACACCCGGTTTATTTCCCCACGCCCCCCTGGTTTTTCGAGGGGGGATTTTTTTTTTTTTCCTCTTTTCCCCCCCCCCTTCAAAAtttttttcttttaatttacatttttaacc                                                                                                                                                                                         >  2:282991/1‑215 (MQ=255)
                                             cATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                                                                                                                      <  1:830877/238‑1 (MQ=255)
                                             cATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                                                                                                                      <  1:1056192/238‑1 (MQ=255)
                                                      aaCCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAACTCATTGGCGGTTGTGGGTCTACCGCCCACTTAATCTCTCCCAAAAATTGGCTGACAAAGGGGTCATTGCCCGTCGACCTCTGGATTCTCCGAGGGCGGATTTGTGTTTATGTTTTTCTTTGCCCCGCCCGCGAAAATATGTTCACTTTTTATTCACTTTTCTTACTACaatctctat                                                                                                                                                                             >  2:461796/1‑230 (MQ=255)
                                                      aaCCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTGGGGCCCACTGCACAATAAATCTCTGACAAAAATCGGGGAAAACCGGTTTTATTTCCCGACGCCCCCCGGGTTTTTCCCGGGGGGATTTGTTTTTTTTTTCTTTTTTCCCCCCCCCGCAAAAAAATTTTCTCTTTTAATTTTAtttttttactaaactccttca                                                                                                                                                                             >  2:206723/1‑220 (MQ=255)
                                                           tGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTCTGGGTCCTCCTGCAAAATAATTTTTTTACAAAAATCGACTGACCCCGGGTTTCTTCCCCCACGCCCCTTGGATTTTTCGCCGGAGTATTTGTTTTTTGTATCATTCTGTCCCCCACCCTAGCAATATTTTTAACTTTATATTCAttttatttattcatccacttcaccca                                                                                                                                                                         >  2:270167/1‑216 (MQ=255)
                                                              tAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAgcg                                                                                                                                                                    <  2:739724/238‑1 (MQ=255)
                                                                       ctctTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTGGGGCCCACTGCACAGTAAATCTCTGACAAGAATAGGCTAACACCGGTTTAATTTCCCGACGCCCCCTGGGTTTTCCCAGGGCGGATTTGTTTTTATGTCCTTCTTGCCCCCCCCCGTAAAAAAATTTTCACTTTAAATTTAATTTTTTAATTACAATCATTTACCACAGCCCGACCCtcgt                                                                                                                                                          >  2:923116/1‑236 (MQ=255)
                                                                       ctctTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTGCGTCCCTCCGCAAACTAAATTTCTTACATAAATAGCCTAACACCCGTTTTTTTGCCCCACCCACCCTCGACTTTCCGTGGGCGTCACTGTTTCTTTTTCCCTCTTGCCCCCCCCCGCACAAATCTTTTTCCTTTTACTTTCATTTTTTTACTACCACCTCTAACCACAACGCCATCCtct                                                                                                                                                           >  2:589825/1‑236 (MQ=255)
                                                                                  aCCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                                                                                                                      <  1:287498/201‑1 (MQ=255)
                                                                                          cACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGACCACAGCAAAATAAAACTCTGACAGAAATTAGCTAAAAACGCGCTAATTTACCGCCACCCCCGGTATTTTTCGCGGAGGCATTTTTTTTTATTTTGTTCTTTCCCCCCCCCCCAAAAATATTTTTCACTTTAATTTAAATTTATAAATACATTCCTTTAACAACACGCGATTCTGTTCGAGAtttttttgtttt                                                                                                                                         >  2:346388/1‑229 (MQ=255)
                                                                                           aCCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcct                                                                                                                                      <  1:891456/239‑1 (MQ=255)
                                                                                                 catgttctatttctaAAAGCAGTGGCGGTTGCGGTCCTACTGGACAGTAAGTCTCTGACAGAGATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAAGTTGATAACTACATCCATGAGCCACAGCTCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                                                                                                                 <  2:944223/234‑1 (MQ=255)
                                                                                                 cAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                                                                                                                 <  1:407453/238‑1 (MQ=255)
                                                                                                 cAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                                                                                                                 <  2:104351/238‑1 (MQ=255)
                                                                                                     ttctatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACa                                                                                                                            <  1:398125/239‑1 (MQ=255)
                                                                                                      tctatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACAt                                                                                                                           <  1:1168895/239‑1 (MQ=255)
                                                                                                      tctatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACAt                                                                                                                           <  1:923116/239‑1 (MQ=255)
                                                                                                      tctatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACAt                                                                                                                           <  1:282991/239‑1 (MQ=255)
                                                                                                       ctatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATa                                                                                                                          <  1:704276/239‑1 (MQ=255)
                                                                                                       ctacttctaAAATCATTGGCGGTGTCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACAACGGTTTAATTGCCCGACGCCCTCTGGAGTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCTCTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATa                                                                                                                          <  2:858327/239‑1 (MQ=255)
                                                                                                        tatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATaa                                                                                                                         <  1:1103166/239‑1 (MQ=255)
                                                                                                              taAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAAcatcat                                                                                                                   <  2:139137/239‑1 (MQ=255)
                                                                                                               aaaaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCata                                                                                                                  <  1:428754/239‑1 (MQ=255)
                                                                                                                 aagcATTGGCGGTTGCGGTGCTACTGCACAGTTAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGATGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCTCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAtg                                                                                                                <  2:1081316/236‑1 (MQ=255)
                                                                                                                 aaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAtg                                                                                                                <  1:270167/239‑1 (MQ=255)
                                                                                                                 aaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAtg                                                                                                                <  1:314089/239‑1 (MQ=255)
                                                                                                                 aaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAtg                                                                                                                <  1:206723/239‑1 (MQ=255)
                                                                                                                 aaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAtg                                                                                                                <  2:460045/239‑1 (MQ=255)
                                                                                                                          cGGTTCCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACTCCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTtata                                                                                                        <  2:1057355/238‑1 (MQ=255)
                                                                                                                               gccgTCCTACTGCACAGTAAGTCTCTTACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATtcatc                                                                                                  <  2:430131/236‑1 (MQ=255)
                                                                                                                                     cTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCTGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCAt                                                                                            <  2:512750/239‑1 (MQ=255)
                                                                                                                                                 aaGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTa                                                                                  <  1:60123/237‑1 (MQ=255)
                                                                                                                                                  aGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCt                                                                               >  1:841787/1‑239 (MQ=255)
                                                                                                                                                   gTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCtt                                                                              >  1:796578/1‑239 (MQ=255)
                                                                                                                                                     ctctGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCtttt                                                                            >  1:543001/1‑239 (MQ=255)
                                                                                                                                                          aCAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATg                                                                       >  1:935959/1‑239 (MQ=255)
                                                                                                                                                                  cGGCTAACACCTGTTTAATTGCCCGTCGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCaaa                                                               <  2:1037896/239‑1 (MQ=255)
                                                                                                                                                                    gCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAAc                                                              >  2:502688/1‑238 (MQ=255)
                                                                                                                                                                     cTAACTCCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAAct                                                             <  2:1102114/238‑1 (MQ=255)
                                                                                                                                                                     cTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCACATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAAct                                                             <  1:346388/238‑1 (MQ=255)
                                                                                                                                                                       aaCACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGTCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACtctc                                                          <  2:518172/239‑1 (MQ=255)
                                                                                                                                                                         cacCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTccc                                                        <  1:502688/239‑1 (MQ=255)
                                                                                                                                                                         cacCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTccc                                                        <  2:935959/239‑1 (MQ=255)
                                                                                                                                                                             ctgTTTAATTGCCCGACGACCTCTGGATTTTACGAGGGCGTATGTGTTTCTATTTACTTCTTGCCCACGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTa                                                     <  2:561552/237‑1 (MQ=255)
                                                                                                                                                                               gTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACa                                                   >  2:432987/1‑239 (MQ=255)
                                                                                                                                                                                    aTTGCCCGACGCCCTCTGGATTTTCCGAGGTCGTATTTGTTTCTATTTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTa                                              <  1:546850/239‑1 (MQ=255)
                                                                                                                                                                                    aTTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTa                                              >  1:141026/1‑239 (MQ=255)
                                                                                                                                                                                        cccGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTgg                                          >  1:847273/1‑239 (MQ=255)
                                                                                                                                                                                                        aTTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTa                            >  2:67701/1‑238 (MQ=255)
                                                                                                                                                                                                           ttCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGc                        <  1:461796/239‑1 (MQ=255)
                                                                                                                                                                                                            tCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATACCTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTtata                                                                                                        <  2:538948/158‑1 (MQ=255)
                                                                                                                                                                                                            tCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTtata                                                                                                        >  1:538948/1‑158 (MQ=255)
                                                                                                                                                                                                                   gCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATggg                  <  2:543001/237‑1 (MQ=255)
                                                                                                                                                                                                                          ttGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAgg          <  1:676735/239‑1 (MQ=255)
                                                                                                                                                                                                                               ttCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAAc       >  1:1153450/1‑238 (MQ=255)
                                                                                                                                                                                                                                tCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTCTAAATTCAATTAGATAACTACATCCATTAGCAACAGCGCGATCCTGTGCGAAATTTTCTGCTCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCTTTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCACTCCAAAGTATTGACCAAGAAAAAGTTATAGCTATGGGATGCCAGGTACt      >  2:1095406/1‑238 (MQ=255)
                                                                                                                                                                                                                                     tGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTATATTCAATTTGATAACTACATACATTAGCCACAACGCGATCCTTTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGATACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGCTATCGCTATGGGAAGCCAGGACCTCCaa  >  2:178940/1‑238 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   
GCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAA  >  NC_000913/3499467‑3499928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: