Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,598,925	A→C	11.5%	F245V (TTT→GTT)	livJ ←	branched‑chain amino acid ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,598,925	0	A	C	11.5%	239.3 / 2.2	85	F245V (TTT→GTT)	livJ	branched‑chain amino acid ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (58/14);  new base C (6/6);  total (64/21)
Fisher's exact test for biased strand distribution p-value = 3.17e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAGGCCGTCCTGCACCGCTCGCGCCAGACCTTCGCCGTATTGCTGTTTGTCGTGAAC  >  NC_000913/3598691‑3599159                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             tttCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGa                                                                                                                                                                                                                                          <  1:171892/239‑1 (MQ=255)                       cttCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGCTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCACTGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGG‑CCCATAAACTGAGTTTTCAGCCCTCACGCGGGt                                                                                                                                                                                                                     >  2:121065/2‑238 (MQ=255)                       aTTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAACCTGAGTTTTCAGCCCTGCCGCGCGt                                                                                                                                                                                                                     >  1:167895/1‑238 (MQ=255)                       aTTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCATCCCTGCCGCGCGt                                                                                                                                                                                                                     >  1:1189316/1‑238 (MQ=255)                       aTTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGt                                                                                                                                                                                                                     >  1:609798/1‑238 (MQ=255)                        ttCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTg                                                                                                                                                                                                                    <  1:126253/238‑1 (MQ=255)                         tCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGcc                                                                                                                                                                                                                  <  1:1032549/239‑1 (MQ=255)                             gCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGcc                                                                                                                                                                                                                  <  1:1043491/235‑1 (MQ=255)                             gCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGcc                                                                                                                                                                                                                  >  2:1043491/1‑235 (MQ=255)                             gCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGAc                                                                                                                                                                                                              >  1:971939/1‑239 (MQ=255)                               ccGCCTGCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCCCACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGc                                                                                                                                                                                                            <  2:1067254/239‑1 (MQ=255)                                 gCCTGCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAg                                                                                                                                                                                                          >  1:109075/1‑239 (MQ=255)                                  ccTGCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACCCCTTCCGGC‑CCCCTAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTgcctccg                                                                                                                                                                                                          >  2:1088225/1‑232 (MQ=255)                                  ccTGCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGCGTTTTCAGCCCTGCCCCGCGTGCCTGACGCAgg                                                                                                                                                                                                         >  1:878484/1‑239 (MQ=255)                                  ccTGCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAgg                                                                                                                                                                                                         >  1:489425/1‑239 (MQ=255)                                  ccTGCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAgg                                                                                                                                                                                                         >  1:824517/1‑239 (MQ=255)                                  ccTGCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAgg                                                                                                                                                                                                         >  1:905916/1‑239 (MQ=255)                                  ccTGCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGGTTTCAGCCCTGCCGCGCGTGCCTGACGCAg                                                                                                                                                                                                          >  2:398364/1‑238 (MQ=255)                                  ccTGCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACATGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCAACAGCAGACCTTCCGCTGATTCGCACGCTCTGTT‑AGAACGCCGACCGTT‑AGCCACACCTCACGCT‑CCCATAACATGTGTTTACCGCCCTGCCGCGCCTGCCTGACGCAgg                                                                                                                                                                                                         >  2:1152780/1‑239 (MQ=255)                                    tGCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGAt                                                                                                                                                                                                       <  1:654573/239‑1 (MQ=255)                                     gCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGAtt                                                                                                                                                                                                      <  1:737491/239‑1 (MQ=255)                                         agatgtgaGCTCGGCTTAGGTGGTCCAAAGAAATGCGCCCCTGGGGTCCTGTTTTGTCGCTTTGATCGCGTTAACACGGGGTTTGTCCGCCGGAACCT‑GATCGTAGTTCT‑TCGTCTTGGTCACCAGCAGCCCTTCCGCTGATTTGCCCGCAATGTT‑AGTCAGCGAAACGTT‑AGCCACACCTTCCGGC‑CTCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTCGcc                                                                                                                                                                                                  <  2:273074/232‑1 (MQ=255)                                         aGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGcc                                                                                                                                                                                                  <  2:202144/239‑1 (MQ=255)                                                aGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑CGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGCGCTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑ttt                                                                                                                                                                                           >  1:645994/1‑238 (MQ=255)                                                    cggcgTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCggg                                                                                                                                                                                      >  2:490425/1‑239 (MQ=255)                                                     ggcgTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCATCCCTGCCGCGCGTGCCTGACGCCGGATTTGCCCCA‑TTTCCCGGt                                                                                                                                                                                     >  1:407408/1‑239 (MQ=255)                                                                 ccAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCTCTGTT‑AGTCAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCCCCGGCCTGCCGCCGGATTTGCCCCA‑TTTTCGGGTG‑ATAACCGCCgt                                                                                                                                                                        >  2:996168/1‑239 (MQ=255)                                                                 ccAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAACCTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCgt                                                                                                                                                                        >  1:346823/1‑239 (MQ=255)                                                                 ccAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTTCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCgt                                                                                                                                                                        >  2:735987/1‑239 (MQ=255)                                                                 ccAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCCGGATTTGCCCCA‑TTTCCGGGTG‑CTAACCGCCgt                                                                                                                                                                        >  1:569579/1‑239 (MQ=255)                                                                 ccAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCgt                                                                                                                                                                        >  1:83763/1‑239 (MQ=255)                                                                 ccAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGCG‑CTAACCGCCgt                                                                                                                                                                        >  1:515038/1‑239 (MQ=255)                                                                 ccAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGACTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAACGTT‑CGACAGCGACACGTT‑AGCCACACCTTCCGGC‑CCCACAACCTGAGTTTTCAGCCCTGCCGCCCGTGCCTGACGCAGGACTTGCTCCA‑TTTCCGGGTG‑ATAAcggccgg                                                                                                                                                                        >  2:637354/1‑238 (MQ=255)                                                                   aaaCGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTCTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGTTTGGTCACCAGCAGCCCTTCCGCTGATTCTCCCGCAATGTT‑AGACAGCGAAACGTT‑ATCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCCGACGCAGGACTTGCCCCA‑TTTCCGGGTG‑CTAACCGCCgaag                                                                                                                                                                      >  2:1086653/1‑239 (MQ=255)                                                                     aCGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTTCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑CTAACCGCCgtagta                                                                                                                                                                    >  2:789762/1‑239 (MQ=255)                                                                         aTGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGTCAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATACACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCACCA‑TTTCCGGGCG‑ATACCCGCCGTAGTAAACg                                                                                                                                                                >  2:617719/1‑239 (MQ=255)                                                                          tGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACCCCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGCG‑CTAACCGCCGTCGTAAACGa                                                                                                                                                               >  2:1069201/1‑239 (MQ=255)                                                                          tGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGCGTTTTCAGCCCTGCCGCGCGTGCCTGCCGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGGAAACGa                                                                                                                                                               >  1:338449/1‑239 (MQ=255)                                                                          tGCGCCACTTGGGTCCTG‑TTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGaa                                                                                                                                                              >  1:42466/1‑239 (MQ=255)                                                                                                   gttctGAGCGCATCAACAGTGGTTTCTTCCGCCGGACCTT‑GATCGTTGTTTT‑TGGGTGTGGTCCCCCGCCCCCCTCCCGCTTATCCCCCCGAAATGTT‑AGACAGCAAACCGTT‑ACCCCCCCCTCCCGCC‑CCAAAAACCGGTGTTTCCGGCCCTCCCGCGGGGGCCTGACGAAGGTTTTCCCCCT‑TTCCGGGGTG‑AAACCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGAcg                                                                                                                                      <  2:813611/235‑1 (MQ=255)                                                                                                      ttGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGAcgcg                                                                                                                                    >  1:1028077/1‑238 (MQ=255)                                                                                                        gagggcgTAAACAGTGGGTTGGTCCGCC‑GAACCTGGCTCG‑AGGTCTGGCGGCTGGGTCCCCAGCGCCCCTCCCGTGAATCCGCCCGAAGTGTT‑AGACGGGACACCGTC‑ACCCCCCCCTCCCGCC‑CCAAAAACCTGATTTTGCACCCCTGCCGCGCGTCCCTGACGAAGGTTTTGCCCCT‑TTCCGGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGcca                                                                                                                                 <  2:83763/235‑1 (MQ=255)                                                                                                               tCAACAATGGGTTTGTTCGCCGGCACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGGCGCAGGATTTGCCCCA‑TTTCCGGTTG‑ATAACCGCCGTAGTCAACGAAGTCGCTATTCTCTTTTTTCAGACGCCCCAACAGc                                                                                                                            >  2:1107423/1‑237 (MQ=255)                                                                                                               tCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGCG‑ATAACCGCCGTAGTAAACGAAGTAGATATTCTCTTTTTTCAGACGCGCCACCAGCGt                                                                                                                          >  2:848115/1‑239 (MQ=255)                                                                                                                        ggTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGACGTCGATATTCTCTTTTTTCAGACGCGCCCCCAGCGTCGAGaaa                                                                                                                   >  2:1115078/1‑237 (MQ=255)                                                                                                                        ggTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGACGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGaaa                                                                                                                   >  1:1048917/1‑237 (MQ=255)                                                                                                                         gTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCt                                                                                                                >  1:818953/1‑239 (MQ=255)                                                                                                                             gTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGACATCtttt                                                                                                             >  1:554167/1‑238 (MQ=255)                                                                                                                             gTTCGCCGGAACCT‑GATCCTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGTGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGCTTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAACCGAAGCCGCTATTCTCTTTTTTCTGACGAGCCAACCGCGTTGAGAAAttcttt                                                                                                             >  2:950464/1‑233 (MQ=255)                                                                                                                                  ccGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGGTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGTGAAACCTTTTTcccc                                                                                                        >  1:381555/1‑238 (MQ=255)                                                                                                                                   cGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCATCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCCCACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGAGCCTGCCGCAGCATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAGACGAAGTAGATATTTCCTTTTTTAAGACGTGCCACCAGCGTTGTGGAATCTTTTTccccc                                                                                                       >  2:268304/1‑237 (MQ=255)                                                                                                                                             aTCGTAGTTCT‑TCGGCTTGGTCACCAGAAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGAATTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGGAGTAAACGAAGTCGATTTTCTCTTTTTTCAGACGCGCCGCCAGAGTTGAGAAATCTTTTTCCCCGGCGGTGATg                                                                                              >  2:1131125/1‑238 (MQ=255)                                                                                                                                                    ttct‑tcGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCaaa                                                                                      >  1:868612/1‑239 (MQ=255)                                                                                                                                                       t‑tcGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGa                                                                                    >  1:368511/1‑238 (MQ=255)                                                                                                                                                       t‑tcGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGCCGGCGATGCCCTCAAAGa                                                                                    >  1:977603/1‑238 (MQ=255)                                                                                                                                                          cGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATCTTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAACTCTTTTTCCCCGGCGGTGATGccccctaacacc                                                                                  >  2:599202/1‑228 (MQ=255)                                                                                                                                                          cGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAac                                                                                  >  1:153745/1‑238 (MQ=255)                                                                                                                                                           ggttctgccccgcgcaccCCTCCCTCTGATCCCCCCAAATTGTG‑AGCAAGCGAAAGTTC‑ACCCCCCCCTTCCGGC‑CCAAAAACCGGTTTTTCCGGCCCCGCCCCGCGTGCCTGCCGCAGTTTTTCCCCCT‑TTCCGGGGTG‑ATACCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAacac                                                                                <  2:1027785/226‑1 (MQ=255)                                                                                                                                                                ggTCACCAGCAGCACTTCCGCCGCTTCGCCCGTCATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAACCTGAGTTTTCAGCCCTTCCGCGCGTCCCTGACCCCGGCTTTGCCCCA‑TTTCCGGCAG‑ATAACCGCCGTAGTTAACGCAGTCGATATTCCCTTTTTTCAGACGCGCCACCACCGTTGAGAAAACTTTTTCCCctgcgtggtatacgctgatgaacaccac                                                                             >  2:227444/1‑214 (MQ=255)                                                                                                                                                                           gcCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCt                                                                >  1:681442/1‑239 (MQ=255)                                                                                                                                                                            cccTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGTCGGTGATGCCATCAACAACCCCCCCGTTTGCTTTTCCtt                                                               >  2:445404/1‑239 (MQ=255)                                                                                                                                                                                tCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTttct                                                            >  1:568259/1‑238 (MQ=255)                                                                                                                                                                                tCCGCTGATTCGACCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAACCTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTGACCGAAGCCGCTATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCACTCATAGAACACCACGTTTACGTTGCCTttct                                                            >  2:893429/1‑238 (MQ=255)                                                                                                                                                                                    gtgcttCGCCCGCAATGTG‑AGCCAGCGAACGTTT‑CGCCCCTCTCCCCGGC‑CCCATAAACGGTGTTTTCCCCCCTGCCTCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCGGGGTG‑ATATCCGCTGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAgg                                                       <  2:109075/235‑1 (MQ=255)                                                                                                                                                                                     cggatccgccGCAATGTTAAGACGCCGAAACGTT‑GCCCACCCCTCCCGCC‑CCCTTAAACTGGTTTTCCACCCCGCCCGCGCGTCCCTGACGC‑GGTTTTTCCCCATTTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAgg                                                       <  2:368511/231‑1 (MQ=255)                                                                                                                                                                                      gtgcatgccccaagagtagtcaaGCGAAACCTTCAGCCCCCCCCTCCGCC‑CCAAAAACCTGTGTTTCCGGCCCCGCCCCGCGGTCGTGACGGAGGTTTTGCCCCT‑TGTCCGGGTG‑AAAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAgg                                                       <  2:681442/217‑1 (MQ=255)                                                                                                                                                                                            cccGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTAATGCCATCAAAGAACACCACGTTTCCATTGCCTTTCTTCAGGCCGTCCt                                                >  1:962152/1‑238 (MQ=255)                                                                                                                                                                                              cGCAATGTT‑AGACAGCCAACCGTT‑ACCCCCACCTTCCGCC‑CCCATAAACTGATTTTTCCGCCCTGCCGCGCGTGCCTGACGCAGTTTTTCCCCCA‑TTTCCGGGTA‑AAAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAGGCCGTCCTGCa                                             <  1:827007/239‑1 (MQ=255)                                                                                                                                                                                                   tGTA‑AGACAGCGAACCGTT‑ACCCCCCCCTCCCGCC‑CCCATAAACTGGGTTTCCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTCCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAGGCCGTCCTGCACCGCt                                        <  1:82944/239‑1 (MQ=255)                                                                                                                                                                                                            aGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAGGCCGTCCTGCACCGCTCGCGCCAg                                >  2:164991/1‑239 (MQ=255)                                                                                                                                                                                                              cGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATTCCATCAAAGAACCCCACGTTTGCATTGCCTTTCTTCAGGCCGTCCTGCACCGCTCGCCCCAgc                               >  2:383368/1‑237 (MQ=255)                                                                                                                                                                                                              cGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAGGCCGTCCTGCACCGCTCGCGCCAGa                               >  1:1146232/1‑238 (MQ=255)                                                                                                                                                                                                              cGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAGGCCGTCCTGCACCGCTCGCGCCAGa                               >  1:884864/1‑238 (MQ=255)                                                                                                                                                                                                              cGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAGGCCGTCCTGCACCGCTCGCCCCAGa                               >  1:1167980/1‑238 (MQ=255)                                                                                                                                                                                                              cGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAGGCCGTCCTGCACCGCTCGCCCCAGa                               >  1:756767/1‑238 (MQ=255)                                                                                                                                                                                                              cGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAGGCCGTCCTGCACCGCCCGCGCCAGa                               >  2:97501/1‑238 (MQ=255)                                                                                                                                                                                                              cGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAGGCCGTCCTGCACCGCCCGCCCCAgc                               >  2:227241/1‑237 (MQ=255)                                                                                                                                                                                                              cGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAAAACACCACGTTTGCATTGCCTTTCTTCAGGCCGTCCTGCACCGCTCGAGCCAGa                               >  2:35162/1‑238 (MQ=255)                                                                                                                                                                                                              cGAAACGTT‑AGCAACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCACTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑CTAACCGCCGTAGTAAACGCAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTTCCCGGCGGTGATGCCATCCAAGAACACCACGGTTGCATTGCCTTTCTTCCGGCCGTCCTGCACCGCTCGCGCCCGa                               >  2:160441/1‑238 (MQ=255)                                                                                                                                                                                                                    cgtgcgcccccccgtCCGTC‑CCAATAAGCTGAGTTTGCACCCCTGCCGCGCGTGCCTGCCGCAGGTTTCGCCCCA‑TTTCGGGTTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAGGCCGTCCTGCACCGCTCGCGCCAGACCTTCg                         <  1:298047/225‑1 (MQ=255)                                                                                                                                                                                                                     cgtgccccccccctCCGGCACCAATAACCTGAGTTTCGCGCCTTCCCGCGCGTGCCTGTCGCAGGATTTCCCCCA‑TTCCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAGGCCGTCCTGCACCGCTCGCGCCAGACCTTCg                         <  1:398364/226‑1 (MQ=255)                                                                                                                                                                                                                              acCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAGGCCGTCCTGCACCGCTCCCGCCAGGCCTTCCCCGTATTGCt               >  2:394322/1‑239 (MQ=255)                                                                                                                                                                                                                                     gcc‑cccATAACCTGTGTTTCCAGCCCGGCCGGGCGGGCCGGACGCAGGATTGCCCCTA‑TTCCCGGGGA‑AAAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAGGCCGTCCTGCACCGCTCGCGCCAGACCTTCGCCGTATTGCTGTTTGTc        <  1:227241/237‑1 (MQ=255)                                                                                                                                                                                                                                       c‑ccaATAAACGGGTTTTCCAGCCCGCCCGCGCGTGCCTGACGCAGTTTTTGCCCCT‑TTTCCGGGAG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAGGCCGTCCTGCACCGCTCGCGCCAGACCTTCGCCGTATTGCTGTTTGTCg       <  1:579448/238‑1 (MQ=255)                                                                                                                                                                                                                                            caacaCTGAGTTTCCAGCCCTGCGGCGCGTGCCGGCCG‑AAGTTTTTCCCCC‑TTTCCGGGGGAATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAGGCCGTCCTGCACCGCTCGCGCCAGACCTTCGCCGTATTGCTGTTTGTCGTGAAc  <  1:421684/235‑1 (MQ=255)                                                                                                                                                                                                                                            aTAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGCG‑ATAACCGCCGTAGAAAACGAAGTCGTTATTCTCTTTTTTCATACGCGACACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCCTCAAAGAACACCAAGTTTGCATTCCTCTTCTTAAGGCCGTCCTGCTCCGCCCTCACCAGACATTCCCCGTTTTGCtagctgtcgagac   >  2:1147721/1‑226 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             TTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTGGTCCAAACGAATGCGCCACTTGGGTCCTGTTTTTTCGCTTTGATCGCGTCAACAATGGGTTTGTTCGCCGGAACCT‑GATCGTAGTTCT‑TCGGCTTGGTCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTT‑AGACAGCGAAACGTT‑AGCCACACCTTCCGGC‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCA‑TTTCCGGGTG‑ATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAGGCCGTCCTGCACCGCTCGCGCCAGACCTTCGCCGTATTGCTGTTTGTCGTGAAC  >  NC_000913/3598691‑3599159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑