Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,665,701 C→A 100% A37S (GCG→TCG)  gadX ← acid resistance regulon transcriptional activator, autoactivator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,665,7010CA50.3% 100.8 / 171.5 102A37S (GCG→TCG) gadXacid resistance regulon transcriptional activator, autoactivator
Reads supporting (aligned to +/- strand):  ref base C (40/10);  new base A (1/51);  total (41/61)
Fisher's exact test for biased strand distribution p-value = 1.05e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.63e-13
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 102 total aligned reads displayed.

TTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATC‑TCTACTA‑CAGTGATGAACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATA  >  NC_000913/3665469‑3665921
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                              
ttACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATC‑TCTACTA‑CAGTGATGAACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATACGCAAAAAc                                                                                                                                                                                                                          <  1:71825/239‑1 (MQ=255)
 tACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATC‑TCTACTA‑CAGTGATGAACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAAcc                                                                                                                                                                                                                         <  1:638548/239‑1 (MQ=255)
      aGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATC‑TCTACTA‑CAGTGATGAACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑c                                                                                                                                                                                                                   >  1:23647/1‑239 (MQ=255)
                  tAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATC‑TCTACTA‑CAGTGATGAACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑CCACGAATACAAAATTTTCAAAACACTTATCTACTCGAA‑TTTGA‑C‑TTG‑CATCAGAAAAACACAGGC‑CA‑CCGCCATTaaa                                                                                                                                                                                                      >  2:515791/1‑239 (MQ=255)
                   aGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATC‑TCTACTA‑CAGTGATGAACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTaaaa                                                                                                                                                                                                     >  2:827978/1‑239 (MQ=255)
                     cGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATC‑TCTACTA‑CAGTGATGAACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAAcc                                                                                                                                                                                                                         >  1:812493/1‑219 (MQ=255)
                     cGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATC‑TCTACTA‑CAGTGATGAACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATa                                                                                                                                                                                                   >  1:742612/1‑239 (MQ=255)
                     cGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATC‑TCTACTA‑CAGTGATGAACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGA‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAACTTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCAGCAAAAACCAGGT‑CA‑CCGCCATTAAAATa                                                                                                                                                                                                   >  2:1162971/1‑239 (MQ=255)
                     cGAAGATTCTTATTCTGCGATAGTTGCGCAACATCCTGAAAAACAGGAATATC‑TCTACTA‑CAGTGATGAACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAAcc                                                                                                                                                                                                                         <  2:812493/219‑1 (MQ=255)
                      aaaGTTTTTTATTCGGCGATAGTGGCGCAACTTCCTGAAAAACAGGAATATC‑TCTACTA‑CAGTGAGAAACGAGTAATGAAAAAAC‑TTTTTT‑ATGTG‑CATGAAGTTTTAATGCCCCCTCCTTG‑AGC‑ATCGGGAAAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCAAA‑TTTGG‑C‑TTG‑CACCCGAAAAAACCAGGT‑CA‑CCGCCATTAAAATAg                                                                                                                                                                                                  <  1:297871/238‑1 (MQ=255)
                             ttttttCTGCGATAGTTGCGCAACTTCTTGAAAAACAGGAATATC‑TCTACTA‑AAGTAATGAACGATTAATAAAAAAAC‑TTTTTT‑ATGTG‑CATAAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAAAAAAAATA‑AAAGCG‑TT‑CCCT‑TTG‑ACACAAATAAAAAATTTTCAACACCCTTATCTACTCAAA‑TTTGG‑C‑TTC‑CACCCGAAAAACCCAGTT‑CA‑CCGCCATTAAAATAGCGATAtt                                                                                                                                                                                           <  1:154305/235‑1 (MQ=255)
                             tttATTCTGCGATATTAGCGCAATTACCTAAAAAACAGAAATATC‑TCTACTA‑CAGTGATGAACGAGAAATGAAAAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAACCCCTCCTCCTTG‑AGC‑ATCGGAAAAAATAATG‑AAAGCG‑TT‑TCCC‑TTG‑ACACGAATAAAAAATTTTCAACACACTTATCTACTCGAT‑TTTGG‑C‑TTG‑CACCCACAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATAtt                                                                                                                                                                                           <  1:364535/238‑1 (MQ=255)
                              tttttgtGCGTTAGTGGAGCACCTTCTGAAAAAATAGAAAACTC‑TCAACAA‑AAGTGAAAAACAATTAATGAAAAAAT‑TTTTTA‑ATGTG‑AAGAAAGATTAAACCCCCCCCCCTTG‑AGC‑ATCGGAAAAAAAAATG‑AACGCG‑TT‑CCCC‑TGG‑ACACAAATAAAAAATTTACAACACCCTTATCTACACAAT‑TTGGG‑T‑TTG‑CACCCGAAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTc                                                                                                                                                                                          <  2:286910/233‑1 (MQ=255)
                              ttATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATC‑TCTACTA‑CAGTGATGAACGAGTAATGAAGAGAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTc                                                                                                                                                                                          >  1:526444/1‑239 (MQ=255)
                              ttATGCTGAGATAGTTGCGCAACTTCCTAAAAAACAGGAATATC‑TCTACTA‑CAGTGATGAACGAGTAATGAAAAAAT‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCCCCTTG‑AGC‑ATCGGGAAAAATAATA‑AAAGCG‑TG‑TCCT‑TTG‑ACACGAAAACAAAATTTCCAACACACTTATCTACTCGAA‑TTTGG‑C‑TGC‑CACCCGAAAAACCCAGGT‑CA‑CCGCCATTAAAATAGCGATATTc                                                                                                                                                                                          <  1:304914/239‑1 (MQ=255)
                               tATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATC‑TCTACTA‑CAGTGATGAACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTc                                                                                                                                                                                          >  2:169544/1‑238 (MQ=255)
                                   cgtcGATAGTTGCGTAATTTCCAGAAAAAGAGAATTATC‑TATACTA‑AATTCATGAGCAATTAA‑GAAAAAACTTTTTTT‑ATGGG‑CATAAAGATTTAGTGCCACCCCCGTA‑GGC‑AGGGGAAGAAAAATTG‑AAGTCT‑TG‑TCCT‑TTG‑ACACAAAAAAAAAATTTTCAACCAATTTATCT‑CTCCAAGTTTGG‑C‑TGC‑CACCCCAAAACACGAGTT‑CT‑CCGCCATTAAAATAGCGATATTCACCAt                                                                                                                                                                                     <  2:1083034/236‑1 (MQ=255)
                                    tGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATC‑TCTACTA‑CAGTGATGAACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCAccct                                                                                                                                                                                     >  1:469355/1‑236 (MQ=255)
                                    tGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATC‑TCTACTA‑CAGTGATGAACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCAtt                                                                                                                                                                                    >  1:656871/1‑239 (MQ=255)
                                      cGATAGTTGCGCAACTTCCTGAAAAACAGGAATATC‑TCTACTA‑CAGTGATGAACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTa                                                                                                                                                                                   >  1:491320/1‑238 (MQ=255)
                                      cGATAGTTGCGCAACTTCCTGAAAAACAGGAATATC‑TCTACTA‑CAGTGATGAACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTa                                                                                                                                                                                   >  2:154305/1‑238 (MQ=255)
                                      cGATAGTTGCGCAACTTCCTGAAAAACAGGAATATC‑TCTACTA‑CAGTGATGAACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTa                                                                                                                                                                                   >  1:367585/1‑238 (MQ=255)
                                      cGATAGTTGCGCAACTTCCTGAAAAACAGGAATATC‑TCTACTA‑CAGTGATGAACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAAAAAAATTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTACAATAGCGATATTCACCATTa                                                                                                                                                                                   >  2:1090063/1‑238 (MQ=255)
                                        aTAGTTGAGCACCTTAGTGAAAAAAAGAAATATC‑TATAATAGAAGTGA‑GAAAGAATAAAAAAAAAAT‑TTTGTT‑ATCGA‑TATGAAGTTTTAATCCCCCCTCTTTG‑CGA‑ATCGGAAAAAATAATA‑AAAGCG‑TG‑TCCC‑TTG‑CCCCAAACACAAAATTTACAACAACCTTATCTACTCAAA‑TTTGT‑C‑TTG‑CATCCAAAAAAGCCATGA‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAAc                                                                                                                                                                                 <  2:579396/238‑1 (MQ=255)
                                        aTAGTTACCCAATTTCATAAAAAACAGAAAGTTC‑TATAATA‑CAGTGATGACAGATTAAAAAACAAAC‑TTTTTT‑ATGGA‑CATAAATATTTAATGCCCCCTCCTTG‑AGA‑ATCGGGAGAAATAATG‑AAGGCG‑TT‑TCCC‑TTG‑AAACAAATAAAAAATTTCAAACACATTTATATACAAAAT‑TTTGG‑C‑TTA‑CACCCGAAAAACCCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                                                                                                <  2:1053458/239‑1 (MQ=255)
                                             tgccaaatttcataaaaaaCAGAAATATC‑CCTACTA‑CGGTAAGGAACGAGAAATAAAAAATT‑TTTTTT‑AGGTG‑CAGAAAGTTTAAATCCCTCCTCCTTG‑AGC‑ATGGGGAAAAATAATG‑AAAGCG‑TG‑CCCC‑TTA‑ACACAAATAAAAAATTTTCAACCCTCTTATCTACCCAAT‑TTTGG‑C‑TTC‑CACCCCAAAAACCCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGt                                                                                                                                                                           <  1:90927/225‑1 (MQ=255)
                                             tGCGCAAATACCTGAAAAACAGGAATATC‑TCTACTC‑AAGTGATGAACGAGTAATAAAAAATC‑TTTTTA‑ATGTG‑AAAGAAGTTTTAATGCCTCCTCCTTG‑AGC‑TCCGGAAAAAATAATG‑AAAGCT‑TG‑CCCG‑TTG‑ACACAAATAAAAAATTTCCAACACCCTTATCTACCCAAA‑TTTGG‑C‑TGG‑AATCCTAAAAACCCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGt                                                                                                                                                                           <  1:696913/239‑1 (MQ=255)
                                             tGCGAACCTTCCTAAAAAACAGAAATATC‑TTTACAA‑CGGTGATGAAGGAGAAATGAAAAAAC‑TTTTTT‑ATGTG‑CATGAAAATTAAATGCCCCCTCCTAG‑AGC‑AGCGGGAAAAAAAAGA‑AAAGCG‑TG‑TCCC‑TTA‑ACAAAAATAAAAAATTTTCAACACATTTATCTACTCGAT‑TTTGG‑T‑TTT‑CATCCAAAAAACCAAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGt                                                                                                                                                                           <  1:426869/239‑1 (MQ=255)
                                             tGCCCAATTTACTAAAAAACAGTAATATT‑TCTAAAA‑CAGTGATGAACTAGTAAAAAAAAAAC‑TTTTTT‑ATGGA‑CATGAAGATTAACTGCCTCCCCCTAA‑AGA‑ATAGGAAAAAAAAATA‑AAACCG‑TC‑TCCC‑TTA‑ACACAAAAAAAAAATTTCAAACACACTTATCTACTCAAA‑TTGGG‑C‑TTC‑CCCCAACAAAAACTAGGC‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGt                                                                                                                                                                           <  2:393481/239‑1 (MQ=255)
                                              gaccaaacttatGAAAAAAAAGAAAAACTTCTACAA‑AAGTGATGAACGAGTAATGAAAAAAT‑TTTTTT‑ATGTG‑AATGAAGATTAAATCCCTCCTCCTTG‑AGA‑ATCGGGAGAAAAAAAA‑AATGCG‑TG‑TCCT‑TTG‑ACACAAATACAAAATTCTCAACACACTTATCTACACAAT‑TTTGG‑T‑TTG‑CACCCGAAAAAACCGGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGt                                                                                                                                                                           <  2:409403/228‑1 (MQ=255)
                                               cgcAACTTCCTGAAAAACAGGAATATC‑TCTACTA‑CAGTGATGAACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTga                                                                                                                                                                         >  1:716471/1‑239 (MQ=255)
                                                caaatttccgaaaaaaCAGGAATATC‑TCTACTA‑AAGTGAGGAACGAGTAATGAAAAAAC‑TTTTTT‑AGTGG‑CATAAAGATTTAAGCCCTCCCCCTTG‑AGC‑AGCGGGAAAAAAAATG‑AAAGCG‑TC‑TCCT‑TTG‑ACACAAAAACAAAATTTTCACCCCACTTACCTACTCAAA‑TTTGG‑C‑TTG‑CACCCACAAAAACCAGTT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTga                                                                                                                                                                         <  1:262921/227‑1 (MQ=255)
                                                  aagttccaaaaaaaaAGAAATATC‑TATACTA‑GAGTGATGAAGGAGTATAGAAGAAAC‑‑TTTTT‑ATGTGAAATAAAAATTAAATGCCTCCTCATGA‑AGC‑AAGGGAAAAAATAAGA‑AAGGCG‑TG‑TCCC‑TGT‑AAACAAACAAAAAATTTACAACCCACTAATCTACTGAAA‑TTTGC‑T‑TGG‑CACCCGAAAAAACGAGGC‑GA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                                                                                                      <  2:177860/230‑1 (MQ=255)
                                                         taaaaaaCAGAAATATC‑TCTACTA‑CAGTGATGAACAAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATA‑AAAGCG‑TG‑TCCC‑TTG‑ACACAAATACAAAATTTTCAACACCTTTATCTACTCAAA‑TTGGG‑T‑TTC‑CATCCACAAAACCCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAttt                                                                                                                                                               <  1:1131100/237‑1 (MQ=255)
                                                         taaaaaaCAGAAATATC‑TCTAAAA‑AAGGGATAAACGAGTAAGGAAAAAAT‑TTTTTT‑ATGTG‑AAGGAAGATTTAATCCCCCCTCCTGG‑AGC‑ATCGGGAAAAATAATG‑AAAGCG‑TG‑TCCT‑TTA‑ACACAAAAACAAAATTTCAAACACTCTTATCTACAAAAA‑TTTGG‑C‑TTG‑CACCCGAAAAAACGGGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAttt                                                                                                                                                               <  2:12851/237‑1 (MQ=255)
                                                                  ggAATATC‑TCTACTA‑CAGTGATGAACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGc                                                                                                                                                      >  1:414682/1‑239 (MQ=255)
                                                                     atgtc‑taTACTA‑AAGTGATAAACGTGAAATGAAAAAAC‑TTTTTT‑ATGTG‑AATAAAGATTAAAGCCCTCCCCCTGG‑AGC‑ATCGGGAGAAATAATA‑AAGGCG‑TG‑TCCT‑TTG‑ACAGGAAAAAAAAATTTCCAACCCTCTTATCTACTAAAT‑TTTGT‑T‑TTG‑CACCCGAAAAAACCGGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                                                                                                   <  2:400466/236‑1 (MQ=255)
                                                                      atttatctACTA‑AAGTGATAACCGAGTAATGAAAAAAT‑TTTTAT‑AGGTG‑ATTAAATTTTTAATCCCTCCTCCTGG‑AGC‑ATGGGGAAAAATAATA‑AAAGTG‑TG‑CCCT‑GTG‑ACACAAATAAAAAATTTAAAACCCCCTTATCTCCCCAAA‑TTGGG‑T‑TTG‑CATCCACAAAAACAAGGC‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                                                                                                   <  2:207424/234‑1 (MQ=255)
                                                                       atC‑TCTACAA‑AGGTTATAAAGGAATAATAAAAAAAT‑TTTTTT‑ATGTG‑CATGAAGATTTACTCCCTCCTCCTAG‑AGA‑ATCGGGAAAAATAATA‑AAAGCG‑TG‑TCCT‑TTG‑ACAAAAAAACAAAATTTTCAACACTCTTATCTACTAAAT‑TTTGG‑T‑TTG‑CACCAAAAAAAACGAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACg                                                                                                                                                 <  2:367585/239‑1 (MQ=255)
                                                                         gtgtgtgtataaGTGATGGA‑GAATAAAGAAAAAAA‑TTTTTTAAGGTG‑CAAGAAGATTAAACCCCTCTCCCAGT‑ATC‑GTGGGAAAAAATAAT‑‑AAATGG‑TG‑TCCCGTTG‑AAAAAAAAAAAAATTTTCCAACACCATCATCTACAAAAA‑TTTGC‑T‑TTG‑CACCCAAAAAAACCGAGC‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                                                                                                   <  2:477371/226‑1 (MQ=255)
                                                                           tctATTA‑CAGTGATGAACGAGTAATGAAGAAAT‑TTTTTT‑ATGTG‑CATAAAGTTTTAATCCCACCTCCTTG‑AGC‑AGCGGGAAAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTCCAACACCCTTATCTACTCGAT‑TTGGG‑C‑TTC‑CACCCGAAAAACCCAGGC‑CA‑CCGCCATTAAAATAGCGATATTCACCAt                                                                                                                                                                                     <  1:1013552/200‑1 (MQ=255)
                                                                                 taaagtaaaaaaCGATAAATGAAGAACG‑TTTTTA‑ATGTC‑CAGAAATATTTACTCCCCCCCCCTTG‑AAC‑ATCGGGAAAAAT‑ATT‑AAACGG‑TGTTCCT‑TTG‑ACCCAAATACAAAATTTCCAACACCTTTACCTACT‑AAA‑TTTGGTC‑TTG‑ACCCCGAAAAACCGAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGa                                                                                                                                         <  2:464978/229‑1 (MQ=255)
                                                                                 a‑cAGTGATGAACATGTAATGACAAATT‑TTTTTT‑ATCGG‑CAAAAAGTTTTAGAGCCCCTTCGTGT‑AGC‑TGCGGAAAAAAAAAAA‑AAAGTT‑TT‑CCCC‑TTA‑ACACAAAAAAAAAATTTTCAACCCATTTTTCTAGACAAA‑TTTGT‑T‑GTT‑CTCCCAAAAACACCAGTT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                                                                                                        <  2:716471/239‑1 (MQ=255)
                                                                                   cAGTGATGAACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACa                                                                                                                                       >  1:200149/1‑239 (MQ=255)
                                                                                    attgataaACGAGTAATGAAAAATC‑TTTTTT‑ATGTG‑CATGAAGATTTAATCCCTCCTCCTTG‑AGA‑ATCGGGAAAAATAATG‑AAAGCG‑TT‑CCCT‑TTG‑ACACAAATAAAAAATTTTAAACACACTTATCTACTCAAT‑TTTGG‑T‑TTG‑CATCCGAAAAACCCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                                                                                                      <  1:827978/237‑1 (MQ=255)
                                                                                    aggaataaaagattaataaAAAAAT‑TTTTTT‑ATTTG‑CATAAAGATTAAATGCCCCCCCCTTG‑AGC‑ATCGGGAAAAATAATG‑AAAGCG‑TG‑TCCT‑TTG‑ACAAGAAAAAAAAATTTTCAACCCCCTTATCTCCTGAAT‑TTTGG‑C‑TTG‑CATCCAAAAAACCCGGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                                                                                                      <  1:662154/226‑1 (MQ=255)
                                                                                    aGTAATAAACGAGAAATGAAAAAAC‑TTTTTT‑ATGTG‑CATGAAGATTAAATCCCTCCTCCAAC‑ACC‑ATCGGGAAAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACAAATACAAAATTTCCAACACCTTTATCAACACGAA‑TTTGC‑C‑TTG‑CACCCGAAAAAACGAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                                                                                                      <  2:491320/239‑1 (MQ=255)
                                                                                      tgatgaACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGt                                                                                                                                                                           >  2:1013552/1‑200 (MQ=255)
                                                                                      tgatgaACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAt                                                                                                                                     >  1:913316/1‑238 (MQ=255)
                                                                                      tgatgaACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAt                                                                                                                                     >  1:1183178/1‑238 (MQ=255)
                                                                                       tattaaCGAGTAATGAAGAACT‑TTTTTT‑ATGTG‑TGAGAATATTAAATTCCATCCCTTTG‑CGC‑ATCGGGAAAAAAAATG‑AAGGCT‑TG‑TCTG‑TGA‑GCACGTTTCCAAATGTCACACCCCACTTCTACAGACGAA‑TTT‑C‑A‑TTT‑AACTCAAAAAAACCATGTCCC‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAtt                                                                                                                                    <  2:595250/234‑1 (MQ=255)
                                                                                       tatgaACTAGTAATGAAAAATC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCCCCTCCTTG‑AGC‑ATCGGGAAAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACCCTTATCTACCCAAA‑TTGGG‑C‑TTG‑CATCCGAAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                                                                                                      <  1:772415/235‑1 (MQ=255)
                                                                                        atgaACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatatat                                                                                                                                                                 >  1:1182405/1‑208 (MQ=255)
                                                                                        atgaACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTc                                                                                                                                                          >  1:209861/1‑215 (MQ=255)
                                                                                        atgaACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTc                                                                                                                                   >  1:433424/1‑238 (MQ=255)
                                                                                        atgaACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTc                                                                                                                                   >  1:477067/1‑238 (MQ=255)
                                                                                        atgaACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTc                                                                                                                                   >  1:698349/1‑238 (MQ=255)
                                                                                            aagagcattGAAGAAAT‑TTTTTT‑ATGTG‑CATGAAAATTTAATCCCTCCTCCGTG‑AGC‑ATCGGGAAAAAAAATA‑AAAGCG‑TT‑CCCT‑TTG‑ACACAAATAAAAAATTTCCAACACACTTATCTACTCGAT‑TTTGG‑C‑TTG‑CACCCAAAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATg                                                                                                                              <  1:1180818/231‑1 (MQ=255)
                                                                                             cGAGTAAGGAAAAAAT‑TTTTTT‑ATGTG‑CATGAAGATTTAATCCCTCCTCCTTG‑CGC‑ACCGGGAGAAAAAATG‑AACGCT‑TG‑CCCC‑TTG‑ACAAGAAAAAAAAATTTTCAACCCACTTATCTACGCGAA‑TTTGG‑T‑TTC‑CATCCAAAAAACCCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGt                                                                                                                             <  1:94051/239‑1 (MQ=255)
                                                                                               agaaagaaaaaatc‑TTTTTA‑ATGTA‑CATAAAGTTTTAACCCCTCGTTC‑TG‑AAC‑AATGGGAAAAAAAAAGAAAAGTT‑TG‑TCCT‑TTG‑ACAAAAAAAAAAAATTTAAAACCCACTTATCTACAAAAT‑TTTGG‑T‑TTG‑CACCAGAAAAAACGAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGt                                                                                                                             <  2:913316/224‑1 (MQ=255)
                                                                                                gTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatatat                                                                                                                                                                 >  1:1003339/1‑200 (MQ=255)
                                                                                                gTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGt                                                                                                                          >  1:71114/1‑239 (MQ=255)
                                                                                                  aaTGAAGAAAC‑TTTTTT‑ATGTG‑CAAGAATATTTAAGCCCTCCTCCTTG‑AGC‑ATCGGGAAAAATAATG‑AAAGCG‑TG‑CCCC‑TTG‑CCACAAAAAAAAAATTTTCAACACACTTATCTACTCAAA‑TTTGG‑C‑TTG‑CACCCGAAAAAACCAGGT‑CT‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGa                                                                                                                        <  1:1162971/239‑1 (MQ=255)
                                                                                                  aaTAAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGTTTTAATGCCTCCTCCTTG‑AGC‑AGCGGGAAAAATAAAG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTCCAACACACTTATTTACTCAAT‑TTTGG‑C‑TGC‑CACCCGAAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGa                                                                                                                        <  1:497112/239‑1 (MQ=255)
                                                                                                   aggaaagatcttttatt‑ATGGT‑CATGAAGATTTAATACCTCACCTGTA‑ATC‑TAGGGAAAAAATAGTG‑AAA‑CT‑TC‑TCCC‑CTGTAAACGAATACAACATATTCACTCTTCTTTTCTAGTCGAA‑TTTGG‑T‑TAA‑CCGCCAAAAAAAGAGTGG‑CT‑CCGCCATTAAAATAGCGATATTCTCCATTAGCCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGa                                                                                                                        <  2:283600/228‑1 (MQ=255)
                                                                                                   aataaaaaatttttttt‑AGGTG‑CATAAAGATTTAATCCCCCCCCCTGG‑AGC‑CTCGGGAAAAAAAATA‑AAAGCG‑TG‑TCCT‑TTG‑ACACAAATAAAAAATTTCCAACACACTAATCTCCCCGAA‑TTTGC‑C‑TTG‑CATCCGAAAAACCCGGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGa                                                                                                                        <  1:874148/228‑1 (MQ=255)
                                                                                                    taaagaaAC‑TTATGA‑ATATG‑AAGAAAGATTGAAAGCCACCTCCGTG‑AAC‑AACGGGAAAAATAAGG‑AAAGCG‑TG‑CCCT‑TTG‑ACAAAAATAAAAAATTTTAACCACACTTATCTACTAAAT‑TTTGT‑C‑TTG‑CACCCCAAAAAACCAGGT‑CG‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGAtt                                                                                                                      <  2:197161/237‑1 (MQ=255)
                                                                                                      aagaaAC‑TTTTTT‑ATGTG‑AAAGAAGATTTAATGCCTCCTCCTTG‑AGA‑AGGGGGAAAAATAATA‑AAAGCG‑TT‑CCCC‑TTG‑ACAAAAATAAAAAATTTTAAACCCACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CACCCAAAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGc                                                                                                                    <  1:696866/239‑1 (MQ=255)
                                                                                                       aaaaatttttttt‑ATGTG‑CAGAAATATTTAATGCCTCCTCCTGT‑AGT‑ATGGGGAAAAAAAATG‑AAAGCT‑TG‑TCCT‑TTA‑ACACAAAAACAAAATTTAAACCCCATTTATCTACTCTAT‑TTTGG‑C‑TTG‑CAGCCGAAAAACCCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGc                                                                                                                    <  1:880828/232‑1 (MQ=255)
                                                                                                        aaaattttttta‑ATGTG‑CATAAAGTTTTAACCCTTCCACCTTG‑AGC‑ATGGGGAAAAATATGA‑AAAGCG‑TG‑CCCC‑TTG‑ACCAGAATAAAAAATTTCAAACAACCTTTTTTATTCGAT‑TTTGT‑C‑TTA‑CACCCAAAAAAACCGGAT‑CC‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCa                                                                                                                   <  1:1090063/233‑1 (MQ=255)
                                                                                                          ataatattttttattgaaataaaGTTTTAAGCCCT‑CTCCTTGAAAC‑AGCGGAAAAAAAAATG‑AAAGCG‑TG‑TCCT‑TTG‑CCAAAAAAAAAAAATTTCCAACACACTTCTATTC‑AAAA‑TTTGG‑C‑TTGCCACCCAAAAAAACCAGGA‑AA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCa                                                                                                                   <  2:656871/218‑1 (MQ=255)
                                                                                                           aC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGCCAATTCCCATGTAGTGATTGCATAGt                                                                                                               >  1:272411/1‑239 (MQ=255)
                                                                                                           aC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                                                                                               >  1:416173/1‑239 (MQ=255)
                                                                                                           aC‑TTTTTT‑ATGTG‑AATGAAGTTTTAATCCCTCCTCCTTG‑AGC‑ATCGGAAGAAATAATA‑AAACCG‑TG‑TCCC‑TTG‑ACACAAATACAAAATTTTCAACCCACTTATCTACTCGAT‑TTGGG‑C‑TGG‑CACCCGAAAAACCCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                                                                                               <  1:698991/239‑1 (MQ=255)
                                                                                                            attttttt‑ATGTG‑AAGAAAAATTTAATGCCTCCGCCTTA‑AGA‑ATGGGGAGAAATAATG‑AAGGCG‑T‑‑TCCC‑GTGAACATGAAAAAAAAATTTTCAACCCACTTATCTACTCGAT‑TTGGG‑T‑TTG‑CATCCAAAAAAACAAGTT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                                                                                               <  1:157038/237‑1 (MQ=255)
                                                                                                              agaggtgaaaaaaagacagaaTTTAATCCCACCTCCAGA‑AAC‑AGCGGGAAAAATAATA‑AAAGCG‑TG‑TCCC‑TTA‑ACACGAAAAAAAAATTTCCAACACCCTTATCAACAAAAT‑TTTGA‑T‑TTC‑CATCCGAAAAAACAGGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                                                                                               <  2:138896/219‑1 (MQ=255)
                                                                                                                 ttt‑ATGTG‑AATGAAGATTTAACCCCCCCCCCTTG‑AGC‑AGTGGGAAAAATAATG‑AACGCG‑TG‑TCCT‑TTC‑ACACAAATAAAAAATTTCAAACACTTTTATCTACAAAAT‑TTTGG‑T‑TTG‑CACCCAAAAAAACCGGTT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACt                                                                                                          <  2:772748/239‑1 (MQ=255)
                                                                                                                  ttgaTGTG‑CATGAATATTTAATCCATCCTCGTGG‑AGC‑AGCGGGAGAAATAATA‑AAAGCG‑TT‑TCCA‑TTG‑ACACGAATACAAATTTTTCAACCCATTAATCTACTAGAA‑TTTGG‑C‑TTG‑CATCAAAAAAACCCGGAT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACt                                                                                                          <  1:182057/236‑1 (MQ=255)
                                                                                                                   tttagatggaaaaaatttacattcctcctcatAG‑AAA‑AGCGGGAAAAAAATGG‑AAGGGG‑TG‑TCCT‑TGA‑ACACAAAAACAAAATTTCCAACACTCTTATCTACAAAAT‑TGTGT‑T‑ATG‑CATCAACAAAAACTAGAA‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACt                                                                                                          <  2:433424/216‑1 (MQ=255)
                                                                                                                   tatatgtaaaataaGATTTACTGCCTCCTCATTG‑ATC‑CTAGAGAAAAATAAAA‑AAGGTG‑TA‑TCCC‑TTG‑ACAAGAAAACAAAATTTTCAACACTCTTATATACCAGAA‑TCTGG‑C‑TCT‑CATCCAAAAAACCCAGCC‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACt                                                                                                          <  2:377482/227‑1 (MQ=255)
                                                                                                                       gtg‑CATGAATATTTAATCCCTCCTCGTGA‑AAC‑ATGGGAAAAAATAAAG‑AAGGCG‑TC‑TCCC‑TTG‑ACAAAAATAAAAAATTTCAAACACTTTTATCTACTAGAT‑TTGGG‑C‑TTG‑CACCCTAAAAAACAAGGT‑AA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAata                                                                                                     <  1:169544/239‑1 (MQ=255)
                                                                                                                         tgaaggaaaatttatgtcctccgcctGA‑AGC‑ACCGGAAAAAATAA‑G‑AAAGGGTTG‑TCCC‑TGA‑AAACAAATAAAAAATTTCCACCACACTTTTCTACCCGAA‑TTTGG‑C‑TGC‑CACCCGAAAAAACCAGGT‑AA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                                                                                                    <  1:31759/222‑1 (MQ=255)
                                                                                                                          gtaagAATATTTAATCCCTCCGCCTAG‑AAC‑ACAGGAAGAAAAAATG‑AAAGCG‑TC‑TGCT‑TGT‑ACATGAATACAAAAGTTTAAACCCCCTTATCTTCT‑GAA‑TTTTG‑CTTTG‑CCCCCAAAAAAACGGGGT‑CT‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                                                                                                    <  2:698349/234‑1 (MQ=255)
                                                                                                                          gcagaaaaaTTTAATCCCCCCT‑CTTG‑GGCAATCGGAAAAAAAAATG‑AAACCT‑TT‑TCCC‑TTG‑AAACGAATAAAAAATTTTAAACACCCTTATCTACTCAAT‑TTTGT‑C‑TTG‑CATCC‑CAAAAAACCGGT‑CATCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                                                                                                    <  1:459941/230‑1 (MQ=255)
                                                                                                                           cATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATAtt                                                                                                   >  1:151845/1‑238 (MQ=255)
                                                                                                                              aaaGATTTAGTCCCTCCCCCGTG‑ACA‑ATCGGAAAAAAAAATA‑AAAGCG‑TG‑TCCT‑TTG‑ACACAAATAAAAAATTTACAACCCACTTACTCACTAAAA‑TTTGG‑T‑TTG‑ACTCCAAAAAAACCGGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                                                                                               <  1:774404/238‑1 (MQ=255)
                                                                                                                                ataTTTAATCCCCCCTCATAG‑AGA‑ATCGGAAAAAATAATG‑AAAGCG‑TG‑CCCC‑TTG‑ACACGAAAAAAAAATTTCCACCCACCTTATCTCCTCAAA‑TTTGT‑T‑TTG‑CATCCGAAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                                                                                               <  2:200149/235‑1 (MQ=255)
                                                                                                                                aGATTAAACCCCTCCCTCTTG‑AGC‑AGCGGGAAAAAAAATA‑AAAGCG‑TG‑TCCT‑TTA‑ACAAAAAAAAAAATTTTACAACACATTTATTTCCTCAAA‑TTTGG‑C‑TTG‑CATCCGAAAAAACCAGGC‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATaa                                                                                             <  1:486532/239‑1 (MQ=255)
                                                                                                                                 aattaaacccccccactatG‑AGA‑ATGGGAAAAAAAAATA‑AAGGCG‑TG‑TCCA‑TTA‑ACAAAAAAAAAAAATTTACAACACCCTTATCTACTCAAT‑TTGGG‑T‑TTG‑CACCCAAAAAAACGGGGT‑AA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATaaa                                                                                            <  2:414682/221‑1 (MQ=255)
                                                                                                                                            tcctcctTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGtt                                                                                                              >  1:719350/1‑210 (MQ=255)
                                                                                                                                            tcctcctTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAata                                                                                                     >  1:138896/1‑219 (MQ=255)
                                                                                                                                                        c‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTa                                                                                                                            >  1:110101/1‑185 (MQ=255)
                                                                                                                                                                        tG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGc                                                                                                                                                >  1:132657/1‑150 (MQ=255)
                                                                                                                                                                         g‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGAACGCCGCCCTCTTa                                                      >  1:923484/1‑239 (MQ=255)
                                                                                                                                                                                  tg‑tCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTaaaa                                                                                                                                                                                                     >  1:341974/1‑89 (MQ=255)
                                                                                                                                                                                    gtacc‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAat                                              >  2:613138/4‑239 (MQ=255)
                                                                                                                                                                                                 cGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAata                                                                                                     >  1:405479/1‑173 (MQ=255)
                                                                                                                                                                                                   aaTACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTa                                                                                     >  1:958461/1‑187 (MQ=255)
                                                                                                                                                                                                                           taataatttaaaattggatttG‑CATCGAAAAAAACTGGGT‑AA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGaa            <  2:1183178/220‑1 (MQ=255)
                                                                                                                                                                                                                                  gAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGTACGCCGCCCTCTTAAGTCAAATTGATTGATAATTAAATTTAAACGAAGCGGGAGAACAGGATGATa  >  2:508850/1‑239 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                              
TTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATC‑TCTACTA‑CAGTGATGAACGAGTAATGAAGAAAC‑TTTTTT‑ATGTG‑CATGAAGATTTAATGCCTCCTCCTTG‑AGC‑ATCGGGAGAAATAATG‑AAAGCG‑TG‑TCCC‑TTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAA‑TTTGG‑C‑TTG‑CATCCGCAAAAACCAGGT‑CA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATA  >  NC_000913/3665469‑3665921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑