Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,782,918 T→C 34.8% D248G (GAT→GGT)  gpsA ← glycerol‑3‑phosphate dehydrogenase (NAD+)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,782,9180TC34.8% 55.5 / 7.8 56D248G (GAT→GGT) gpsAglycerol‑3‑phosphate dehydrogenase (NAD+)
Reads supporting (aligned to +/- strand):  ref base T (16/15);  new base C (4/12);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCA  >  NC_000913/3782688‑3783152
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                            
aTGCTGCCTCGCGGGTGTTTTTCCCGCATTATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCCCCTGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCCCCTGACAAATCTTCTCCTGCGCGTTTTGTCCACCCATGCCCTGACCGAGCATCATGCCAAACCGGCGGTTCCGCGCCTGGTTGTCGGTACAGGTAACCCCCAGATCGCCAAGc                                                                                                                                                                                                                                      <  2:975316/239‑1 (MQ=255)
 tgctgcCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGGTGTCGGGACAGGGAAACACCAGATAGcccaac                                                                                                                                                                                                                                      >  1:883398/1‑234 (MQ=255)
      ccTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACCGGCCCCGTTTTCGGTGAAGGCAAACACCAGAACACCAAGACCCGc                                                                                                                                                                                                                                 >  2:327306/1‑238 (MQ=255)
       cTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGGCTGGTTGTCCGCAAAGGGAAGCACCAAAACGCCGAGCCCCGcc                                                                                                                                                                                                                                >  1:471190/1‑238 (MQ=255)
       cTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTAAGCGGCTGGTTGTCGGTACAGGGAAGCACCAAATCCCCAAGCcccccc                                                                                                                                                                                                                                >  1:688456/1‑238 (MQ=255)
                gTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTTCCGATAAGCACCAAACCGCCCAGCCCCGCCCTGCCCCTac                                                                                                                                                                                                                     >  1:608963/1‑238 (MQ=255)
                          cAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACCGGTTGTCGGGGGAGGCAAGCACCACACCGCGCAGCCCCGccccacccctcaaagtgggcag                                                                                                                                                                                                            >  2:831241/1‑219 (MQ=255)
                                  taCTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTCGCGACTGGTTTTCGGTACACGGAAACACCAGAACGCCAAACCCCCCCCAGCCCAAAAAGGGGGcggaggcgtga                                                                                                                                                                                                    >  1:609440/1‑228 (MQ=255)
                                       gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGCCTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAACCGGCGGTTTCGCCCCACGGTTGTGGCGCCGGAGATCACCACATCGCCAATGCCCGCCCTGCCCCTAAACGTGCCAGGGGCGGCcccccg                                                                                                                                                                                               >  2:921255/1‑236 (MQ=255)
                                       gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTTTCGGGACCGGGAAGCACCAAATCGCCAAACCCCGCCCTGCCCATAAAGGGGGGAGGGTCGGGAcccc                                                                                                                                                                                                >  1:11189/1‑237 (MQ=255)
                                       gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTACGGACTGGTTGTCGGCACAGGTAAGCACCAGACCACCCAGCCCCCCCAGCCCCCTAAATGGGGCAGCGTGGGCAacccc                                                                                                                                                                                               >  2:598322/1‑236 (MQ=255)
                                       gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTACCGGCCTGTTGTCCGTAAAGGGAAACACCAAAACCCCAAACCCCCCCACCGCCCTAAAAGGGGGCGGGGCGGGCACCCa                                                                                                                                                                                                >  2:1092939/1‑238 (MQ=255)
                                       gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGGCTGGTTGTCGGTACAGGTAAACACCAAATCGCCAAGCCCCCCCCTGCCCCTAAAAGGGGCAGGGGCGGCCCCCAgc                                                                                                                                                                                              >  1:68709/1‑239 (MQ=255)
                                       gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGGACAGGTAAGCACCAAATCGCCAAGCCCCCCCACGCCCATAAAGGGGGGACGGGCGGGACCCAgc                                                                                                                                                                                              >  1:189451/1‑239 (MQ=255)
                                       gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGGTGTCGGGGCAGGGAAACAACAAATCCCCAAACCCCGCCATGCCCCTAAAAGTGGCAGGGGCGCGAcccca                                                                                                                                                                                               >  1:967795/1‑236 (MQ=255)
                                        aTAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGGACAGGTAAGCACCACAACGCCAAACCCCGCCATGCCCCTAAAAGGGGCAGGGGCGGCACCCacc                                                                                                                                                                                              >  1:405357/1‑236 (MQ=255)
                                         tAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTCGCCCCTGGTTGTTGGGACAGGTAAGCACCAAATCGCCAAGCCCCGCCCTCCCCTCAAAGGCGGCACGGTCGGCACCCAccg                                                                                                                                                                                             >  2:63457/1‑238 (MQ=255)
                                         tAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGCGCAGGGAAACACCAGATCGCCAAGACCCGCCATGCCCATAAAGGTGGCATGGTTGGATCCCacct                                                                                                                                                                                             >  1:320022/1‑235 (MQ=255)
                                                       tATGGGCATTTCAGCGCCGAAGCGTTGCGCCATTCCTCGGACTCCTTTCGTTTTGCGGTACCCTCCCACCCCCTGACCAATCTTCCCCGGCGCGCTTTGTACATCCATGCCCTGACCGAGCTACATGCCAAACCGGCGGTTACGCGCCTGTTTTTCGGTACAGGTAGGCCCCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGAcgc                                                                                                                                                                              <  2:467317/238‑1 (MQ=255)
                                                                ttCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTTGGGACAGGTAAGCACCAAAACGCCAAGCCCCCCCCTGCCCCTAAAGGTGGCAGGGTCGGCACCCAACGCCGCACCaaacagcgaccattca                                                                                                                                                                     >  1:109317/1‑224 (MQ=255)
                                                                ttCAACGCCGAAGCGATGCGCCAGTTCGAGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTCTCGGCACGGGTTTTCTCTGCAGGGAAGCACCAAACCACCACGACCCGCCATCCCCCCAGAGCGGGCAGGGGCCGCCACCAGCCGCCCCCCCAGAcgccgcacatca                                                                                                                                                                     >  2:555810/1‑229 (MQ=255)
                                                                 tCACCGCCGAGGCGATCCGCCAGTGCGCGGCCTTCTTCGTTATTGCGGTGGCCTTCCACCACCTGACCATTCTGCTCCGGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGGGACTGGTTGTCGGTACAGGTAAGCACCAGACCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTcagc                                                                                                                                                                   <  2:703578/239‑1 (MQ=255)
                                                                 tCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTcagc                                                                                                                                                                   <  1:1093905/239‑1 (MQ=255)
                                                                                             ggccttcttTCGTATTGCGGTAGCCTCCCACCACCTGACCAATCTCCTCCTGCGCGCTTTGTACACCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTGCGCGACTGGTTGTCGGTACAGGTAAGCACCAGACCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTAcg                                                                                                                                        <  1:508833/235‑1 (MQ=255)
                                                                                                    tttCGTATTGCGGTAGCCTCCCCCCCCCTGCCCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCg                                                                                                                                <  1:941838/239‑1 (MQ=255)
                                                                                                        gTATTGCGGTAGCCTTCCACCCCCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCAGGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGACCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCAcc                                                                                                                            <  1:63457/239‑1 (MQ=255)
                                                                                                         tATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGCACTGGGGTCCGTCAAAGGAAGCACCAAAACGCCAAGCCCCGCCCTGCCCCTCAAGGGGGGAGGGGCGGGACCAAACGCCCCACCCAAGACCGACAATTCAGACAGCCCCCAGGGGAGCAGCCCCGCACACCCCTTCGCCCCa                                                                                                                           >  2:1072251/1‑239 (MQ=255)
                                                                                                                 tctccTTCCCCCCCCTGACCAACCTTCCCCGGCGCGCTGTGTACATCCAGGCCCTGACCGGGCACAACCCCAAACCGGGGGTTCCGCGTCTGGTTGTGGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGa                                                                                                                    <  2:405357/235‑1 (MQ=255)
                                                                                                                          ccccccggACCAATCTTCTCCTGCGCGCGTTGTACACCCAGGCCCGGCCCGACCATCAGGCCAAAACGGCGGTTCCGCGCGTGTTTGTCGGTACAGGTAAGCCCCAGCTCCCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGa                                                                                                          <  2:1100751/232‑1 (MQ=255)
                                                                                                                          ccccccTGCCCAACCTCCCCCGGGGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAACCGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGa                                                                                                          <  1:968163/235‑1 (MQ=255)
                                                                                                                          ccccccTGACAATGCTCCTCCGGCCCGCTTTGACCCTCCATCCCCGGACCAGCCATCAGGCCAAACCGGGGGTTCCGCGCCGGGTTGTGGGTACAGGTAAGCGCCAGACCCCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGa                                                                                                          <  2:109317/235‑1 (MQ=255)
                                                                                                                                      aTCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCAAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCAcc                                                                                              <  1:231348/239‑1 (MQ=255)
                                                                                                                                                 cgcgcTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTATGCGGCCGGTTGTCGGTTCAGGGAAGCACCAGAAAGGCCAGCCCCGCCCTGCCCATAAAAGGGGGAGGGGCGGCACCCAGCGCCGCCCCCAAAAGCGCCAATTAAGGCAGCCCCCGGGTGAGAAGCGCCCGACGCGCACTCCGAACCAAACAGAACCCGTCTGAGATacccccccccagagcaacca                                                                                   >  2:71097/1‑223 (MQ=255)
                                                                                                                                                 cgcgcTTGGTACATCCAGGCCCTGACCGAGCATCAGGCCAAACCGGCGGTTACGCGACGGGTTCTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATa                                                                                    <  1:527888/238‑1 (MQ=255)
                                                                                                                                                             aTCCCTGCCCGTACCGAGCCTTAGGCCAACACGCGGGTGAGGGGACGGTTGCTCGTTACAGGTACGCACCGGATCCCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACcg                                                                       <  2:751799/239‑1 (MQ=255)
                                                                                                                                                             aTCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACcg                                                                       <  1:342028/239‑1 (MQ=255)
                                                                                                                                                             aTCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGCCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACcg                                                                       <  1:1092939/239‑1 (MQ=255)
                                                                                                                                                                ccagccccagcgcgcgagtcatGCAAAAACGGCGCTCCCGCGATGGGTGGACGTAACAGGTACGCACCACCCCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCCCCAATGGCAATAACGTTTTTCGCcgcg                                                                     <  2:549394/221‑1 (MQ=255)
                                                                                                                                                                          ccGAGCATCATGCCAAAGCGGCGGTTACGCGCCTGTTTGCCGGTACAGGTAAGCCCCAGACCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTg                                                          <  1:495532/239‑1 (MQ=255)
                                                                                                                                                                             ggccatcagGCCAAACCGGCGGTTCCGCGACTGGTTGTCGGTACAGGTAAGCACCAGACCCCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCa                                                        <  1:595791/236‑1 (MQ=255)
                                                                                                                                                                                cgtaagcccAAACCGGCGTTTACGCGACGGTTTGCCGGTACGGGAAAGCCCAAGACCGCCAAGCACCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCa                                                        <  1:416768/229‑1 (MQ=255)
                                                                                                                                                                                     tGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCCCCAGAGCGCCAGGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATg                                                <  1:921255/239‑1 (MQ=255)
                                                                                                                                                                                              ggcggTTCCGCGACGGGTTGGCGGTGCAGGTAAGCCCCAGACCGACAGGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGa                                       <  1:606922/239‑1 (MQ=255)
                                                                                                                                                                                                        gcgGCTGGTTGTCGTGACAGGTAAGCCCCAGATCGCCAACCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTaa                              <  1:598322/239‑1 (MQ=255)
                                                                                                                                                                                                                    tgccGGTGCAGGAAAGCACCAGACCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGaa                      <  1:81510/235‑1 (MQ=255)
                                                                                                                                                                                                                               gcaaGCCCCAGACCCCCAACCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGACCCCAGCGCCGCACCAAGCCGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAGCGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCg            <  2:11189/235‑1 (MQ=255)
                                                                                                                                                                                                                                tAAGCCCCAGATCCCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCg            <  1:1072251/236‑1 (MQ=255)
                                                                                                                                                                                                                                  aGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGt        >  1:517045/1‑238 (MQ=255)
                                                                                                                                                                                                                                    cacaAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGa                                                                                                                    <  1:1171738/128‑1 (MQ=255)
                                                                                                                                                                                                                                    cACCAGCTCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCATCGCCGTACGCGCATTCGCACCAAACCCGCTACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCCCGCCACTGAAATCCGGCTTGCTGTAAACCCGGAGACTTTTGCCGCAGTgca     >  2:388047/1‑239 (MQ=255)
                                                                                                                                                                                                                                    cACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGa                                                                                                                    >  2:1171738/1‑128 (MQ=255)
                                                                                                                                                                                                                                    cACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGCGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCCGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACCTCCCCGCACCAATGGCAATAACGTTATTCACCGCCCCGCCATCCTGCACGCCACTGAAATCCGGATTGCTGTAACCTCGGAAACTTTTGCCGCAGTgc      >  2:975882/1‑238 (MQ=255)
                                                                                                                                                                                                                                      ccAGACCGCCACGCCGCGCCATGGCCCGAAAGGAGCCAGAGTCGGGTCTCAGCGCCGCACCAAGACGCGCCATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAATCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCGGCCAAGCTGGACGCCGATGAAATCCGGATTGCTGTAAACGCGGAATCTTTTGCCGGAGTgcagc   <  2:250488/239‑1 (MQ=255)
                                                                                                                                                                                                                                         gATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgcagca  >  1:226212/1‑237 (MQ=255)
                                                                                                                                                                                                                                         gATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGCACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGCTTGCTGTAACCGCGGACACTTTTGCCGCAGTgcggca  >  2:151646/1‑237 (MQ=255)
                                                                                                                                                                                                                                         gATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGACACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgcagca  >  2:257281/1‑237 (MQ=255)
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ATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCA  >  NC_000913/3782688‑3783152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: