Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,862,672	G→A	25.9%	pseudogene (931/1617 nt)	glvC ←	pseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,862,672	0	G	A	25.9%	42.8 / 12.5	59	pseudogene (931/1617 nt)	glvC	pseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component
Reads supporting (aligned to +/- strand):  ref base G (19/22);  new base A (11/2);  total (33/26)
Fisher's exact test for biased strand distribution p-value = 2.38e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.54e-02

GGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AA‑GGTTGCGGG‑AATCAGC‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCAAAGA‑TTTTTGAGTTACCGTG‑CAGGGCAAAACCG‑CCTT‑CCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAAG  >  NC_000913/3862439‑3862904                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                        ggTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGAAACTT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAA‑GGTTGC‑GG‑AATCAgacag                                                                                                                                                                                                                                                     >  1:764632/1‑234 (MQ=255)       catcatATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑TA‑GGGTGCGGG‑GATCAAC‑AAGCCCG‑cc                                                                                                                                                                                                                                             >  1:402749/1‑238 (MQ=255)            tatCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑GT‑AA‑AGTTTCGGG‑AATCAGC‑CAACCCC‑CCC‑C‑cttt                                                                                                                                                                                                                                     >  1:209819/1‑239 (MQ=255)                 gcgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑GT‑AA‑GGGTGCGGG‑AAACAAC‑AAACCCC‑CCA‑C‑TTTTT‑cccc                                                                                                                                                                                                                               >  1:260670/1‑238 (MQ=255)                  cgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCCAACAGCAACGGCGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGAAATTCCCACCAGCATGGC‑GG‑GT‑AA‑GGGTGCCGG‑GATCCAC‑AAGCCCG‑CCA‑C‑ATTTA‑cccc                                                                                                                                                                                                                               >  2:1061460/1‑237 (MQ=255)                  cgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGCGGG‑TT‑AA‑GG‑TGCGGG‑GAACAAG‑AAGACCG‑CCC‑C‑tttttaccca                                                                                                                                                                                                                               >  1:1064912/1‑233 (MQ=255)                  cgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑GT‑GA‑GAGTTCTGG‑AAACAGC‑aaaactcccccactcacccca                                                                                                                                                                                                                                  >  2:596831/1‑220 (MQ=255)                      gatGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAACTT‑CCAGCGGTGCGGACATTCACACCAGCATGGCGGG‑AA‑AA‑AGTTGCGGG‑AAACAGC‑CAGCCCG‑CAC‑C‑ttttcaccgtgtt                                                                                                                                                                                                                            >  2:768914/3‑230 (MQ=255)                         gCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑CT‑AAGGGTTGCGGG‑AAT‑ACC‑AAAACCC‑CCC‑C‑CTTTA‑TCCGCTTTTCCg                                                                                                                                                                                                                       >  1:430932/1‑239 (MQ=255)                           tGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AA‑GGGTGGGGG‑GAACTGCAAAACCCC‑CCC‑T‑TTTTA‑CCGGATTTCCCgt                                                                                                                                                                                                                      >  1:1206568/1‑238 (MQ=255)                                 tCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGCGGG‑GA‑AA‑GGTGCCGGG‑CAACACC‑AACCCCC‑CCC‑C‑TTTAC‑CCCGATTTTCCgtgccgcc                                                                                                                                                                                                                >  2:442019/1‑232 (MQ=255)                                  cGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAA‑GGTTCGGGG‑ATAAAAC‑AAACCGC‑CCC‑C‑TTTTA‑CCCATTTTTCTGGGGGGGcg                                                                                                                                                                                                               >  1:142706/1‑238 (MQ=255)                                   gcaaagaaGTTTTGCGATAAAGCGTAGCGAATCACGCCCACGCCGGTGTTCCCCCCCACAAAGAAGAGAAAAAGCACGCGCGAGATTGAGGCCACGAGAAGGGCGTGTACCGAAAACGAAAGCGGTAAAATGAGGAGGAGGATAAATT‑CCATGGGTGCGGAATCCCCCACCAGCAGGGT‑GG‑TT‑AA‑GGTGGGGGA‑ATTCAGC‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCgg                                                                                                                                                                                                                 <  2:222549/228‑1 (MQ=255)                                       tCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AA‑GGGTGGGGG‑GATCAAC‑AAGACCG‑CCA‑C‑TTTTT‑CCCCATTTTTCGGTGCggggaggaag                                                                                                                                                                                                         >  1:712708/1‑231 (MQ=255)                                              ttGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑TA‑GGGTTAGGG‑GATAAAG‑AAAACCG‑CCA‑A‑CTTTT‑CCCCATTTTCCGGGGCGGGCGGGAAGTaaacc                                                                                                                                                                                                   >  1:595969/1‑234 (MQ=255)                                               tgcgcaaaaaCCTAGTCAATCAACCCCCCCCCCATGTCCCCCACCACACCAAAGAGAAACATTACGGACGACAATGGGGCCGCCACCACGGCGTGCACCGAAAGCAGCAACGGTAAAATGAAGAGGAAGGTAATTC‑CCAGCGGTTGGGTACCCCCCACCAGCAGGGG‑TG‑TT‑GA‑GGTGGGGGA‑AATAACC‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGc                                                                                                                                                                                                <  2:402749/233‑1 (MQ=255)                                                 cGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AA‑GGTTGGGGG‑GATCAAC‑AAAACCC‑CCA‑C‑TTTTT‑ACCGGGTTTCCCGTGCGGGAGGGAAGTA‑CACCGccc                                                                                                                                                                                              >  1:926009/1‑237 (MQ=255)                                                 cGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGGAATTCCCACCAGCATGGC‑GG‑TT‑AA‑GGGTGCGGG‑GATTAGC‑AAGCCCC‑CCA‑C‑TTTTA‑TCCCGTTTTCCGTTGCGGCatgggaagacaatccca                                                                                                                                                                                               >  1:347422/1‑223 (MQ=255)                                                              tCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AA‑AGTTGCGGG‑GATCAGC‑AAGCCCC‑CCACC‑TTTTC‑CCCGTTTTTCC‑GTGCGGCCGTGAAGGA‑AAACGC‑TC‑AAGAA‑AAGCCCa                                                                                                                                                                               >  1:785806/1‑238 (MQ=255)                                                                       ccgcggcgcgtGTTGCCCCCCACACAGAGGAGATAGATTGCGTTGCATGTTGGGGCCCCCATCGCGGGCTATGCCGCAAACAGAAGGGGGGAAGTGATCAGGAAGTGAAGTC‑CCAGCGGCGCGGTATGTCCCCCGAGCAGGGG‑TT‑TT‑AA‑GGTGGAGGA‑AATCAGC‑ATCCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCC‑CGGCG‑CCaa                                                                                                                                                                    <  2:112315/228‑1 (MQ=255)                                                                           cGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AA‑AGTTGCGGG‑AATCAAC‑AAGCCCG‑CCA‑CATTTTA‑CCCGTTTTTCCGG‑GCGGCCGGGAAAGA‑CATAGC‑TA‑AAAAA‑AAACCCACCGGCA‑CAAAAGA‑t                                                                                                                                                               >  1:547921/1‑239 (MQ=255)                                                                           cGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑GT‑AA‑AGATGGGGG‑GATCAGC‑AAACCCC‑CCC‑C‑CTTTA‑CCCCGTTTTTCGGTGCGGGAGTGGAGGA‑AAACGCGTA‑AGGAA‑AT‑CCCA‑Cgggccccaagcat                                                                                                                                                                >  2:291853/1‑228 (MQ=255)                                                                           cGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑GT‑AA‑AGATGCGGG‑GATCAGC‑AAACCCC‑CCC‑C‑TTTTT‑CCCGATTTTTCCTGGGGGCAGGGAAGGA‑CATCGC‑CT‑AAGAA‑AAACCCC‑CCGCC‑CCCAAGaat                                                                                                                                                               >  1:1054497/1‑237 (MQ=255)                                                                                        caccacaccaAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AA‑AGGTTCGGG‑AATCAGC‑AAACCCA‑CCA‑C‑TTTTA‑CCCGATTTTTCGGTTGGGCAGTGGAGTA‑CAACAC‑TA‑AAAAA‑AAGCCCA‑CGGCT‑CCCAAGA‑CTTTTTAGGTaaccg                                                                                                                                                 >  1:1003611/1‑235 (MQ=255)                                                                                                   agagATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑GT‑AA‑AGATGCGGG‑GAACCGC‑AAACACC‑CCACC‑TTTTA‑CCC‑ACTTTTCGGTGCGGGACGGAAAGA‑CAACCA‑CA‑AAGAA‑AACCCCA‑CAGCA‑CCCAAGACTTTTTGGGTACCTATC‑CAGGGCaaa                                                                                                                                      >  2:1127342/1‑239 (MQ=255)                                                                                                       aTACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑GT‑AA‑GGGTTCGGG‑AGACAGC‑AAACCCC‑CCC‑C‑CTTTA‑CCCCCTTTTCCGCTGGGGCAGGGCAGTA‑CAACCC‑CC‑AAGAACAACCCCC‑CGCCA‑CC‑AAGA‑CTTTTTTGGTACCATGCCGGGGCAAAAcc                                                                                                                                   >  2:178141/1‑238 (MQ=255)                                                                                                        tACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑GT‑AA‑AGGTTCGGG‑GAGCAGC‑AAGCCCGCCCC‑C‑TTTTA‑CCC‑AGTTTTTGGTGCGGCAGGGAAAGA‑AAACGC‑CC‑AAACA‑ATGCCCC‑CGGCC‑ACAAAAA‑TTTTTTAGTTAccagccaagagcaaaccgc                                                                                                                                  >  2:265192/1‑221 (MQ=255)                                                                                                             tACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AA‑AGTTGCGGG‑GATCAAC‑AAACCCC‑CCC‑C‑TTTTA‑CCCGATTTTTCCGTGCGGGAGGGAAGAA‑CATCGC‑TA‑AAAAA‑ATGCCCC‑CGGCA‑CCAAAGA‑TTTTTGTGTTACCGCG‑CAGGGCaaaaaacccctccc                                                                                                                           >  1:1025253/1‑229 (MQ=255)                                                                                                             tACGGTCGACATTGAGGCCGCCAGCACGGCGTGGACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAAATCCCACCAGCATGGC‑GG‑AT‑AA‑GGTTGGGGG‑GATGCGC‑CAGCCCGCCCA‑C‑TTTAA‑CCC‑ATTTTACGGGGGGGGAGGGGAGGA‑CACTAC‑AACAAAAA‑AGCCCCA‑CGCCA‑CC‑AAGA‑GTTTTGAAAGACCGTG‑CGCGGAAAAAcacccctcac                                                                                                                           >  2:404026/1‑230 (MQ=255)                                                                                                               cGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAAGT‑CCAGCGGTTCGGTAATTCCCACCAGAATGGC‑GGATA‑AA‑GGTTCCGGA‑AAT‑ACC‑AACCGCC‑CCA‑C‑CTTTACCCCGCTTTTCTGGT‑AGGCGGTTTAATAGAATCGC‑AA‑AAGAA‑ATACAC‑‑CGGCACCCACGGA‑TTTTTGAGTTATC‑TT‑CCGGGCaaaaacacccctccgg                                                                                                                         >  2:503344/1‑227 (MQ=255)                                                                                                                  tCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCAACAGCATGGCGGG‑TA‑AA‑GGTTGGGGG‑AAT‑AAC‑AAAACCC‑CCC‑C‑TTTTA‑CCCCATTTTTCGGGGCGGGGGGGGAGCA‑AATCGC‑TA‑AAAAA‑ATATCCA‑CCGCC‑CACCAGA‑GTTTTGTGGTACCGCG‑CAGGGCaaaaacacactcccgggac                                                                                                                      >  2:560639/1‑224 (MQ=255)                                                                                                                     aCATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AA‑GGTTGCGGG‑GATCAGC‑AAGCCCC‑CCC‑C‑TTTTA‑CCCGATTTTTCGGTGCGGCAGGGGAGTA‑CATCGC‑TA‑AAGAA‑AAGCCCC‑CGGCA‑CCCAAGA‑TTTTTTGGTTAACGTG‑CAGGGGAAAACCGCCCTT‑CCGGaaaaa                                                                                                                   >  1:767567/1‑236 (MQ=255)                                                                                                                              ccgccAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGCGGG‑TA‑AA‑GGTTG‑GGG‑AATCAGC‑AAACCCG‑CCC‑C‑CTTTA‑ACCGGTTTTTCGGGGCGGCAGGGAAGTA‑CATCGC‑TC‑AAAAA‑AAGCCCA‑CGGTC‑CCCAAGG‑TTTTTTGGGTAACGGG‑CAAGGCAAAAACC‑CCTTCCCGGGaaaaagcttttcgc                                                                                                         >  2:1194356/1‑230 (MQ=255)                                                                                                                                                  aaaaaCCGCAGCGGTAAAATGAAAGGAAAGTTAAATC‑CCAGCGGTTCGTTAATCCCCCCCAGCAGGGC‑GG‑TT‑AG‑GGTGGGGGA‑AATCAGC‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCAAAGA‑TTTTTGAGTTACCGTG‑CAGGGCAAAACCG‑CCTT‑CCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGaa                                                                                    <  1:47914/237‑1 (MQ=255)                                                                                                                                                   cAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AA‑GGGTGCGGG‑GATCAGC‑AAGCCCG‑CCA‑C‑CTTTA‑CACGCTTTTCAGGGGCGGCAGTGAAGTA‑CATCGC‑TA‑AAAAA‑AAGCCCA‑CGGCA‑CCcaaaa                                                                                                                                                                 >  1:1041919/1‑163 (MQ=255)                                                                                                                                                   cAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AA‑AGGTGCGGG‑AATCAGC‑AAACCCC‑CCA‑C‑CTTTA‑ACCGATTTTTCGGTGCGGCAGTGAAGGA‑CACCGC‑CA‑AAAAA‑AATCCCC‑CGCCC‑CCCAAGA‑TTTTTTTGTTACCGTG‑CAGGGCAAAAACC‑CCCT‑CCGGGAACAAAGATTTTAGCGGCTCGGCGCGCAAAccgcac                                                                                   >  2:834236/1‑234 (MQ=255)                                                                                                                                                    aaaCAGCAACGGTGAAGTGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATCCCCACCGGCATGGC‑GG‑TT‑AA‑GGGTGCGGA‑AATCAGC‑AGCCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCAAAGA‑TTTTTGAGTTACCGTG‑CAGGGCAAAACCG‑CCTT‑CCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAAct                                                                                  <  1:1127342/239‑1 (MQ=255)                                                                                                                                                      aCAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AA‑GGTTGCGGG‑GATCAGC‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGGTTTTTCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCa                                                                                                                                                                     >  1:183287/1‑158 (MQ=255)                                                                                                                                                      aCAGCAACGGTAAAAGAAAAAGGAAGGAAAATC‑CCAGGGGTCCGGTAATCCCCCCCAGCTGGGC‑GG‑TT‑AG‑GGTGGCGGG‑AATCAGC‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCAAAGA‑TTTTTGAGTTACCGTG‑CAGGGCAAAACCG‑CCTT‑CCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAActct                                                                                <  1:291853/239‑1 (MQ=255)                                                                                                                                                                 gAAAGGAACGGGAAGAAAAATT‑CCAGGGGTCCGGTTATTCCCACCAGCATGGC‑GG‑TA‑AA‑GGTGGGGGG‑AATCAGC‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCAAAGA‑TTTTTGAGTTACCGTG‑CAGGGCAAAACCG‑CCTT‑CCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGAtgct                                                                      <  1:1106069/238‑1 (MQ=255)                                                                                                                                                                  aaaTAAACAGGAAGTAAAATT‑CCAGCGGTTCGGAAATCCCCACCAGCATGGC‑GG‑TA‑AG‑GGTTGCGGA‑AATCAGC‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCAAAGA‑TTTTTGAGTTACCGTG‑CAGGGCAAAACCG‑CCTT‑CCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGAtgct                                                                      <  1:8900/237‑1 (MQ=255)                                                                                                                                                                    aataaCAGGAAAGTAAATTCCCGGCGGTCGGGTAATCCCAACCAGCAGGGC‑GG‑TA‑AG‑GGTTGCGGA‑AATCAGC‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCAAAGA‑TTTTTGAGTTACCGTG‑CAGGGCAAAACCG‑CCTT‑CCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTgcg                                                                   <  1:660197/236‑1 (MQ=255)                                                                                                                                                                     tGAACGGGAGGTAAAATC‑CCAGGGTGTGGGTAATTCCCCCCAGC‑TGGC‑GGTTT‑AG‑GGTTGCGGA‑AACCAGC‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCAAAGA‑TTTTTGAGTTACCGTG‑CAGGGCAAAACCG‑CCTT‑CCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc                                                                 <  1:768931/239‑1 (MQ=255)                                                                                                                                                                     tGAACAGGAATGAAAATC‑CCAGCGTTTCGATAATCCCCCCCAGCATGGC‑GT‑GT‑AA‑GGTTGGGGG‑AATCAAC‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCAAAGA‑TTTTTGAGTTACCGTG‑CAGGGCAAAACCG‑CCTT‑CCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc                                                                 <  1:1194356/239‑1 (MQ=255)                                                                                                                                                                       aaCAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AA‑GGTTGCGGG‑AATCAGC‑AAGACCC‑CCA‑C‑TTTTA‑CCCGATTTTTCGGTGCGGCAGGGGAGTA‑CATCGC‑CA‑AAAAA‑AATCCCC‑CCGCA‑CCCAAAA‑TTTTTGAGGTACCGTG‑CAGGGCAAAAAAC‑CCTT‑CCGGGAACAACCATTTTAGCGGCCCGGGGCTCAAACGGAACTCtttccgcagcggggccc                                                                >  2:665205/1‑223 (MQ=255)                                                                                                                                                                          aGGAAGTAAATTT‑CCAGCGTTGCGGTAATCCCCACCAGCTGGGC‑GT‑TT‑AA‑GGTTGTGGA‑ACTCAGC‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCAAAGA‑TTTTTGAGTTACCGTG‑CAGGGCAAAACCG‑CCTT‑CCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTa                                                            <  1:106471/239‑1 (MQ=255)                                                                                                                                                                                     atcccAGCGGTGCGTTACTCCCCCCCAGAAGGGC‑GG‑TT‑AG‑GGTTGCGGA‑AATCAGC‑ACGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCAAAGA‑TTTTTGAGTTACCGTG‑CAGGGCAAAACCG‑CCTT‑CCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATg                                                  <  1:740045/236‑1 (MQ=255)                                                                                                                                                                                        ccagtggtttgtaacatCCCACCAGCAGGGC‑GG‑TC‑AG‑TGTGGGGGG‑AACAGGA‑AACCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCAAAGA‑TTTTTGAGTTACCGTG‑CAGGGCAAAACCG‑CCTT‑CCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATgccg                                               <  1:768914/223‑1 (MQ=255)                                                                                                                                                                                                   ggacaccaccccacggcgggggg‑TT‑AA‑GGGTGGGGGTAACCGGC‑AACCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCAAAGA‑TTTTTGAGTTACCGTG‑CAGGGCAAAACCG‑CCTT‑CCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACa                                      <  2:260670/218‑1 (MQ=255)                                                                                                                                                                                                           cccccgCATGGC‑GG‑TT‑AA‑GGTGGCGGG‑AACCAGC‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCAAAGA‑TTTTTGAGTTACCGTG‑CAGGGCAAAACCG‑CCTT‑CCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGAc                            <  1:665205/234‑1 (MQ=255)                                                                                                                                                                                                             cgagatgggC‑GG‑TT‑AA‑GGTTGCGGG‑AAGCAGC‑AGCCACG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCAAAGA‑TTTTTGAGTTACCGTG‑CAGGGCAAAACCG‑CCTT‑CCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCa                          <  1:657388/232‑1 (MQ=255)                                                                                                                                                                                                             ccaGCATGGC‑GG‑TT‑AA‑GGTTGCGGG‑AATCAGC‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCAAAGA‑TTTTTGAGTTACCGTG‑CAGGGCAAAACCG‑CCTT‑CCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCa                          <  1:721084/239‑1 (MQ=255)                                                                                                                                                                                                               ctctggcagggggttgagtgtggCGGA‑AATCAAC‑AACCCCG‑CCA‑C‑TTCTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCAAAGA‑TTTTTGAGTTACCGTG‑CAGGGCAAAACCG‑CCTT‑CCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGAc                            <  2:933716/217‑1 (MQ=255)                                                                                                                                                                                                               ccaggttgctg‑TT‑TA‑GGCTGGGGGAAATCAGA‑ACGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAATTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCAAAGA‑TTTTTGAGTTACCGTG‑CAGGGCAAAACCG‑CCTT‑CCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCa                          <  1:1182449/229‑1 (MQ=255)                                                                                                                                                                                                                    ggC‑GG‑TT‑AA‑GGTGGCGGG‑AATAAGC‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCAAAGA‑TTTTTGAGTTACCGTG‑CAGGGCAAAACCG‑CCTT‑CCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATa                    <  1:442019/238‑1 (MQ=255)                                                                                                                                                                                                                      c‑GG‑TT‑AA‑GGTTGGGGG‑AATCAGC‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCAAAGA‑TTTTTGAGTTACCGTG‑CAGGGCAAAACCG‑CCTT‑CCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATaaa                  <  1:842811/238‑1 (MQ=255)                                                                                                                                                                                                                       ggg‑TT‑AA‑GGTGGCGGA‑AATCAGC‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCAAAGA‑TTTTTGAGTTACCGTG‑CAGGGCAAAACCG‑CCTT‑CCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATaaa                  <  2:1025253/237‑1 (MQ=255)                                                                                                                                                                                                                        gg‑TT‑AA‑GGTTGCGGA‑AATCAGA‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCAAAGA‑TTTTTGAGTTACCGTG‑CAGGGCAAAACCG‑CCTT‑CCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAAc                 <  1:834236/238‑1 (MQ=255)                                                                                                                                                                                                                                  ggtGCGGG‑AACAACC‑AGCCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCAAAGA‑TTTTTGAGTTACCGTG‑CAGGGCAAAACCG‑CCTT‑CCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGt          <  2:209819/236‑1 (MQ=255)                                                                                                                                                                                                                                           aaTCAGC‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCAAAGA‑TTTTTGAGTTACCGTG‑CAGGGCAAAACCG‑CCTT‑CCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAAg  <  1:86328/238‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                        GGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AA‑GGTTGCGGG‑AATCAGC‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTA‑CATCGC‑TA‑AAGAA‑ATGCCCA‑CGGCA‑CCAAAGA‑TTTTTGAGTTACCGTG‑CAGGGCAAAACCG‑CCTT‑CCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAAG  >  NC_000913/3862439‑3862904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑