Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,003,508 | T→G | 21.6% | T224P (ACC→CCC) | rarD ← | putative chloramphenical resistance permease |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,003,508 | 0 | T | G | 21.6% | 102.6 / 2.2 | 60 | T224P (ACC→CCC) | rarD | putative chloramphenical resistance permease |
Reads supporting (aligned to +/- strand): ref base T (17/26); new base G (14/2); total (32/28) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.17e-03 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TATATTTATTTTTTATTTGGACGTTCTACGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATG‑CCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTAC‑CAGACCGTAGAAGGCAAAACTAAATGCCAGTCCCAGCGCGATAATAGGTAGCGAACCAA > NC_000913/4003275‑4003738 | tatatTTATTTTTTATTTGGACGTTCTACGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTg > 1:512100/1‑238 (MQ=255) tatttGGACGTTCTACGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAAAAGCGGGACGGGAAGGAGAATACGggcggc > 2:722052/1‑239 (MQ=255) tCTACGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGGAAGGTAGTGACAATACCGGCGGCGAACAGCAGTa > 1:22920/1‑239 (MQ=255) gagTAGAAATCGCAGCCATCACAAAAAGGGCCACCGCGACCCAAATAAAGGCGAAAGTCCCCATCTGATCTGCACCCGGTTTTGCACCATAAAACGTCACAGCCAGCAGGAACAGCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCGGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATGCCGGCGGCGATCAGCAGTAAATTCAg < 2:221508/236‑1 (MQ=255) cGTATACATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATACAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAACAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGGAAAAAACAACAGCGGTACGGGAGAGACAAAAGCCGCGGCCGGCAGGAGTAAAATCAACg > 2:902853/1‑239 (MQ=255) cGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGCGACGCAAGCGCGGGGCAGCGGCGGCAAAACACAACAGAGGTCAGGTGGTGACAATACCCGCGGCGGACCGCAGAAAAtcacggc > 2:358979/1‑233 (MQ=255) cGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTGGGGACAATACCGGCGGCGATCAGCAGGAAATTCAGCg > 1:826424/1‑239 (MQ=255) cGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGGACGGTAGTGACAATACCGGCGGCGAGCAGCAGTAAATTCAGCg > 1:720793/1‑239 (MQ=255) cGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACAAAAAAACGGGTCGGTAGGGCACATACCGGGGGCGGTCCGCCCGAAAATCAGca > 2:155679/1‑238 (MQ=255) cGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAAACCAACAGCGCAACGAGAGAGCCGATACCCGCAGACATCAGAAGAAAAATCAGcc > 2:302874/1‑238 (MQ=255) cGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAAAACAACAGCGGGACGGTAGTGACAATACCGGGGGCGATCAGCAGGAAATTCAGCg > 1:358506/1‑239 (MQ=255) gTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTGAAAAACAACAGCGGGACGGTAGTGACAATAACCGCGGGGGTCAGGAGGaaaattcacg > 1:541337/1‑231 (MQ=255) gTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAAAAACGGGACGGGAGGGACAAAACCGGCGGCGAACAGCAGGAAAATCAGcc > 1:230837/1‑237 (MQ=255) gCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCCAGCGCGTGGCAGCGGCGGTAAAAAACCAAAGCGGGACGGTAGAGGCCATACCCGCGGCGGTGAGGCGGAAAATCAGCGAcctaagggt > 2:164876/1‑231 (MQ=255) gCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGtt < 2:512100/239‑1 (MQ=255) gCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGACCGGGAGTGACAAAACCGGCGGGGGCCAGCCGAAAATTCAGGCACACCggggt > 1:450422/1‑237 (MQ=255) tccatcACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCGCCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTg < 1:1053157/239‑1 (MQ=255) gcCACCCAAATAAAGGCGAAAGTCACCAGCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAg < 1:718824/237‑1 (MQ=255) cacaaaGGCGAAACGCACCATCTGAGCGGCACCCGGTTTTTCACCATAAAACGTAACAGCCAGCAGGAACATCAGGGTCGGGCCAATGTACTGGAAAAAGCCTAACGTGGAGAGACGCAAGCGCGTGGCCGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACAGCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCaa < 2:620781/236‑1 (MQ=255) ggCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAAAAGCGGTACGGGAGTGACCCTTACGGCGGGGGACAACAGAAAATTCAGCGACATCGGGAGTTGCCCCACTAGGGTGGTCGAGATGTCGGCaacaaaca > 2:106475/1‑233 (MQ=255) aaaGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGGAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCTGCCATaacaaac‑ac > 2:948045/1‑238 (MQ=255) ccATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACCAAACAATGGTACGGGCAGGACAAAAACCGCGGGGGTCAGTAACAAATTCAACGCAACGGGGGTTTGCCCCCACTTGCTGGGTGAGCTGTCGGGAATCACTAAC‑Agcgaacg > 2:240202/1‑232 (MQ=255) aTCGACGCCCGGTTTTTCACCATAAAGCGGCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCGGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCa < 2:797611/239‑1 (MQ=255) cGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAAAATCGGGAAGGGAGTGACAATACCCTCGGCGATCAGCCGGAAATTCCGCTGCAACGGGGTTTGCCCCATATTGGTGGTTCAGCTGTTGGCAATCACAAAA‑ATGGAAATTGCAGCCACg > 2:134455/1‑239 (MQ=255) cccGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGGAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAACAAAA‑AAGGAAATTGCCGCCACGGgca > 1:976304/1‑238 (MQ=255) ccGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGGAAATTCAGAGACATCCGGTTTTGCCCCATATGGCTGGTTGAACTGTCGGCAATAGCAAAC‑AGGTAAAATGCCGCCACGGgcagc > 1:1098458/1‑239 (MQ=255) ttCACCAAAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTCACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCc‑aca < 1:554688/239‑1 (MQ=255) tAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGCAGGGACAATATCGGCGGCGGTTAGCAGTAAATTCAGCGACATCGGGCTTTGCCCCaca > 1:875733/1‑169 (MQ=255) aaagcgagaAGGAACAACAGCGTGGGGCAAAGGAACTGGAAAAAAACTAACGTGGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAATACAACAGCGGTACGGGAGTGACAATTGCGTGGTGGGTCAGCAGTAAATTCAGCGACATCGGGTTTTGCCGCATAAGGCTGGTTGAGCTGTCGGGAATAGCAAAC‑AGTTAAATTTTCGGCACGGGCAGAAGCC‑ACATGGTTTCGATTAAc < 2:338581/235‑1 (MQ=255) ccagcAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGAAAAACCAAAAAACCGTACGGGAGAGGCAAATACGGGGGCGGTCAGCAGCAAATTCCGGGACATCGGGGTTTTCCCCATATTGGTGGTTGGGCTGTCCGCAATAGCAAAC‑AGGGAAATTGCCGCCACCGGCAGTCGCC‑ACCGTGTTTCTGTTAACAAG‑ccc > 2:1103836/1‑238 (MQ=255) aaCATCAGCGTCGGGCCAAGGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATG‑CCGGTTTg < 1:164876/236‑1 (MQ=255) gCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACCACAGCGGGACGGGAGGGACAATAACGGCGGCGAGCAGCAGGAAATTCAGCGGCATCGGGGTTTGGCCCACATGGCCGGGTGAGATGTCCGCCACAGCAAAC‑AGGGAAATTGCCGCCCCGGGCAGCAGGC‑ACATGGGTTCGCATAAAATG‑CCCGGTTGCCCCTCACCg > 2:35987/1‑239 (MQ=255) gCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGGAAGGTAGTGACAAAACCGGCGGCGGTCAACAGTAAAATCAGCGACATCGGGGTTTGCCCCATATGGCCGGGTGAGATTTCCGCAATAGCAAAC‑AGGTAAATTACCGCCACGGGGGGCAGCCAACAAGGTTT‑GAATAACaaggccgggtgcgcgtccaca > 2:1102995/1‑219 (MQ=255) ggcGGTCCAAGGGACTGAAAAAAGCCTAACTGTGAGAGACGCAATCGCGTGGCAGAGGCTGTAAAATACAAGAGAGGGACGTTAGTGACAAGACCGGCGGCGAGCAGCAGAAAATGCAGCGACATCTGGTGTTGCCCCATATGGCGGGTTGAGCGGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGTC‑ACATGGTTTCGATTAACATG‑CCGGTTTGCGCTTCAACgt < 2:760540/236‑2 (MQ=255) cGGGCAAATGTACTGGAAAAAGCCTAACGTTGGGAGGCGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATG‑CCGGTTTGCGCTTCAACGGCa < 2:976304/238‑1 (MQ=255) cAATGTACTGGAAAAAGCCTAACGTAGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATG‑CCGGTTTGCGCTTCAACGGCAAtcttc < 1:948045/239‑1 (MQ=255) aTGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATG‑CCGGTTTGCGCTTCAACGGCAATcttctt < 1:280155/239‑1 (MQ=255) tGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGcggtacggt > 1:146748/1‑68 (MQ=255) tGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGcggtacggt < 2:146748/68‑1 (MQ=255) tGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAAAACAACAGCGGGACGGGAGAGACAATACCGGCGGCGGGAAGCAGTAAATTCCGCGGCGGCGGGGTTTGCCCCATATGGCGGGTTGGGCGTTCCGCAATAGCAAAA‑CAGTAAATTGCCTCCACGGGGAGACGCA‑CCATGGTTTCGGATAACAAC‑ACGGTTTGGGGGTGAAAGGCAATCTTCTTTCGtc > 2:1174769/1‑237 (MQ=255) tGGAAAAAGACTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATG‑CCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTAc < 2:1085995/239‑1 (MQ=255) aaaaGCCTAACGGTGGGAGGCGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATCCCGGCGGGGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCGTATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATG‑CCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTAC‑CAGa < 2:22920/239‑1 (MQ=255) aGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGGACGGGAGTGACAATACCGGCGGCGGTCAGCAGTAAATTCAGCGACATCGGGGTTTGCCCCATATGGCTGGGTGAGCTTTCGGCAATAGCAAAA‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGGTTCGCTTAACAAG‑CCGGTTTGGGCCTCAACGGCCATCTTCTTGGCTAC‑CCGACCg > 2:977540/1‑239 (MQ=255) aGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAAAAGCGGTACGGTAGTGACAATACCGGCGGCGGTCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCCCGGGGAGCAGCC‑CAATGGTTTCGATTAACATGCCCGGTTTG‑GCCTCAACGGCAATCCTCTTTTGTAC‑Caaaacg > 2:1008510/1‑234 (MQ=255) tGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAATCACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATG‑CCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTAC‑CAGACCGTAGAAGGCaa < 1:209657/239‑1 (MQ=255) tGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATG‑CCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTAC‑CAGACCGTAGAAGGCa < 1:106475/238‑1 (MQ=255) agagaCGCAAGCGCGTGGCAGCGGCGGTAAAACACCACAGCGGGACGGTAGTGACAATACCGGCGGCGATCAGCCGTAAATTCAGCGACATCGGGTTTTTCCCCATATGGCTGGGTGAACTGTCGGCAATAACAAACAAGGGAAATT‑TCGCCCCCGGCCGCAGCC‑ACATGGGTTCGCTTAAACTGCCCGGTTTGGGC‑TCAACGGGAAACTTCTTTTGTACACAAACCGAAGAGGGCaaa > 2:656526/1‑239 (MQ=255) agagaCGCAAGCGCGTGGCAGCGGCGGTAAAAACCAACAGCGGTAAGGGAGTGAAAATAACGGCGGCGATCAGAAATAAAATTAGGGACATAGGGTTTTTCCCCATATGGCTGGTTGTGATGTCGGAGAAAGCAAAA‑AGGTAAATTTTCCCCACGGGAAGAATCC‑CCAACGTTTCCAATTACAAG‑CCGGGTTTCCCGTTAAAGAGAACTTTCTTTCGTAC‑AAAGCCGTAGAAAGCaaa > 2:1073912/1‑238 (MQ=255) gagaCGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATG‑CCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTAC‑CAGACCGTAGAAGGCaaaa < 1:155679/238‑1 (MQ=255) cGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATG‑CCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTAC‑CAGACCGTAGAAGGCAAAACTaa < 1:722052/238‑1 (MQ=255) gCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATG‑CCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTAC‑CAGACCGTAGAAGGCAAAACTAAAt < 1:161928/239‑1 (MQ=255) aaGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACGTCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATG‑CCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTAC‑CAGACCGTAGAAGGCAAAACTAAATGc < 1:1174769/239‑1 (MQ=255) cAGCGGCGGTAAAATACAACAGCGGTACGGGAGTGACAATACCGGCGGCGATCAGCAGTAAATTAAGCGACATCGGGTTTTGCCCCAGATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATG‑CCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTAC‑CAGACCGTAGAAGGCAAAACTAAATGCCAGTCCCAg < 2:541337/238‑1 (MQ=255) cAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATG‑CCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTAC‑CAGACCGTAGAAGGCAAAACTAAATGCCAGTCCCAg < 1:902853/238‑1 (MQ=255) cAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATG‑CCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTAC‑CAGACCGTAGAAGGCAAAACTAAATGCCAGTCCCAg < 1:358979/238‑1 (MQ=255) gcggggGTAAAACACAACGGCGGTACGGTAGTGACAATACCGACGGGGAGCAGCAGGAAATTCAGCGACATCGGGTTTTGCCGCATATGGATGGTTGAGCTGGCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTGCGATTAACATG‑CCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTAC‑CAGACCGTAGAAGGCAAAACTAAATGCCAGTCCCAgcg < 2:12275/238‑1 (MQ=255) ggcggTAAAACACAACAGCGGTACGGTAGGGACAATCCCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATG‑CCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTAC‑CAGACCGTAGAAGGCAAAACTAAATGCCAGTCCCAgcg < 2:937122/236‑1 (MQ=255) ggcggTAAAACACAACAGCGGTACGGTAGGGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATG‑CCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTAC‑CAGACCGTAGAAGGCAAAACTAAATGCCAGTCCCAgcg < 2:230837/236‑1 (MQ=255) caGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGTAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGAt < 2:204395/141‑1 (MQ=255) caGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGAt > 1:204395/1‑141 (MQ=255) caGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATG‑CCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTAC‑CAGACCGTAGAAGGCAAAACTAAATGCCAGTCCCAGCGCGATAATAGGTAGCGaa > 2:1070579/1‑238 (MQ=255) caGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATA‑CCGGTTTGCGCCTCAACGGCAATCTTCTTGCGTAC‑CAGACCGTAGCAGGCAAAACTACATGCCAGTCCCAGCGCAATAATAGGTAGCGaa > 1:559719/1‑238 (MQ=255) cggtacggtaGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATG‑CCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTAC‑CAGACCGTAGAAGGCAAAACTAAATGCCAGTCCCAGCGCGATAATAGGTAGCGAACCaa > 2:290563/1‑239 (MQ=255) cggtacggtaGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATG‑CCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTAC‑CAGACCGTAGAAGGCAAAACTAAATGCCAGTCCCAGCGCGATAATAGGTAGCGAACCaa > 1:1173179/1‑239 (MQ=255) cggtacggtaGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGCTTTGCCCCATATGGCTGGTTGAACTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATG‑CCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTAC‑CAGACCGTAGAAGGCAAAACTAAATGCCAGTCCCAGCGCGATAATAAGTAGCGAACCaa > 1:674691/1‑239 (MQ=255) gttactgtaGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATG‑CCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTAC‑CAGACCGTAGAAGGCAAAACTAAATGCCAGTCCCAGCGCGATAATAGGTAGCGAACCaa < 1:1020762/236‑1 (MQ=255) gggacggtcGTGCCAGTCCCGGTGGCGATCAGCAGCAAACTCAGCGACATCGGGTTTTGCACATTATGGCTGGGTGAGCTGTCGGCAATAGCAAAC‑AGGTAAACTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATG‑CCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTAC‑CAGAGCGTAGAAGGCAAAACTAAATGCCAGTCCCAGCGCGATAATAGGTCGCGAAc < 2:450422/232‑1 (MQ=255) | TATATTTATTTTTTATTTGGACGTTCTACGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAAC‑AGGTAAATTGCCGCCACGGGCAGCAGCC‑ACATGGTTTCGATTAACATG‑CCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTAC‑CAGACCGTAGAAGGCAAAACTAAATGCCAGTCCCAGCGCGATAATAGGTAGCGAACCAA > NC_000913/4003275‑4003738 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |