Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,145,049 T→C 11.0% Y352Y (TAT→TAC)  pflD → putative glycine radical domain‑containing pyruvate formate‑lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,145,0490TC11.0% 408.7 / 9.7 158Y352Y (TAT→TAC) pflDputative glycine radical domain‑containing pyruvate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base T (91/42);  new base C (2/16);  total (94/64)
Fisher's exact test for biased strand distribution p-value = 3.98e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 158 total aligned reads displayed.

CAGTATATGTTGCCGTTCTATCAGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGC  >  NC_000913/4144813‑4145283
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                            
      atGTTGCCGTTCTATCAGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCg                                                                                                                                                                                                                                       <  1:226701/238‑1 (MQ=255)
          tGCCGTTCTATCAGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGCCGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTAACGCCTATCAACGCGTGCaa                                                                                                                                                                                                                                  >  2:1194445/1‑239 (MQ=255)
          tGCCGTTCTATCAGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCaa                                                                                                                                                                                                                                  >  1:283852/1‑239 (MQ=255)
             cGTTCTATCAGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAAtt                                                                                                                                                                                                                                >  1:941782/1‑238 (MQ=255)
             cGTTCTATCAGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAAtt                                                                                                                                                                                                                                >  1:216507/1‑238 (MQ=255)
             cGTTCTATCAGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAAtt                                                                                                                                                                                                                                >  1:1094681/1‑238 (MQ=255)
             cGTTCTATCAGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGCGAAC‑G‑T‑GCTTTCGCTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCACtt                                                                                                                                                                                                                                >  2:145237/1‑238 (MQ=255)
              gTTCTATCAGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTAc                                                                                                                                                                                                                              <  2:473298/239‑1 (MQ=255)
                 cTATCAGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAACTGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGACCGGACGTAGCGCGGTGAAA‑G‑T‑GCTTTCGTTCCTATG‑CCTTGTAGCCTATCAAAACGTGCAATTCCCg                                                                                                                                                                                                                            >  2:774072/1‑238 (MQ=255)
                    tCAGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCCATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGGAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTATG‑CCGTGACGCCTATCAAAGCGGGCACTTACCGCaa                                                                                                                                                                                                                         >  2:1159084/1‑238 (MQ=255)
                     cAGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAAcc                                                                                                                                                                                                                       <  1:965796/239‑1 (MQ=255)
                      aGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGGCGCCTATCAACGAGTGCAATTACCGCAAcc                                                                                                                                                                                                                       >  2:1128298/1‑238 (MQ=255)
                            catTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTc                                                                                                                                                                                                                >  2:284201/1‑239 (MQ=255)
                            catTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTc                                                                                                                                                                                                                <  1:412970/239‑1 (MQ=255)
                             atTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCg                                                                                                                                                                                                               >  1:1107958/1‑239 (MQ=255)
                              ttAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCg                                                                                                                                                                                                               >  1:905314/1‑238 (MQ=255)
                              ttAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCg                                                                                                                                                                                                               >  1:1062492/1‑238 (MQ=255)
                              ttAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATAACGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCCGGGCGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTCCCGCAACCGAACATCg                                                                                                                                                                                                               >  1:907441/1‑238 (MQ=255)
                                  cccAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGCCGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGt                                                                                                                                                                                                           >  1:287656/1‑238 (MQ=255)
                                      gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGACC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTCACGCCTATCACAGCGTGGCATTCCCGCACCCGAACCCCGGCGTgcgc                                                                                                                                                                                                       >  2:852206/1‑238 (MQ=255)
                                      gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCg                                                                                                                                                                                                                            <  1:342676/217‑1 (MQ=255)
                                      gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAAcc                                                                                                                                                                                                                       >  1:397641/1‑222 (MQ=255)
                                      gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAAcc                                                                                                                                                                                                                       <  2:397641/222‑1 (MQ=255)
                                      gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGACACCGAACCTCGGCGTgcgc                                                                                                                                                                                                       >  1:135082/1‑238 (MQ=255)
                                      gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATAACAGCGTGCAATTAACGCAACCGAACCTAGGCGTgagc                                                                                                                                                                                                       >  1:106653/1‑238 (MQ=255)
                                      gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGCAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTGTCAAAGCCTGCAATTACGGCAACCGAACCTCCCCGTgcgc                                                                                                                                                                                                       >  2:1205975/1‑238 (MQ=255)
                                      gggCGAAGATGCGGCGTTCCTGAAAGAACTGCCCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGCTAACCGAGAACGGACGTAGCGCGGTGATC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTccgc                                                                                                                                                                                                       >  1:10909/1‑238 (MQ=255)
                                         cGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACt                                                                                                                                                                                                    <  1:737940/238‑1 (MQ=255)
                                         cGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACt                                                                                                                                                                                                    <  2:905314/238‑1 (MQ=255)
                                         cGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACt                                                                                                                                                                                                    <  1:159532/238‑1 (MQ=255)
                                          gAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTa                                                                                                                                                                                                   <  1:225424/238‑1 (MQ=255)
                                           gtctgatagaacggcaacatataCTGGTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑G‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCACTTACCGCAACCGAACCTCGGCGTGCGCACTAAc                                                                                                                                                                                                 >  1:20900/23‑239 (MQ=255)
                                                 cggcgTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGGGCCATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTg                                                                                                                                                                                           >  1:828852/1‑239 (MQ=255)
                                                      ttCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCg                                                                                                                                                                                       >  1:544447/1‑238 (MQ=255)
                                                        ccTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATc                                                                                                                                                                                                                                             >  2:8072/1‑182 (MQ=255)
                                                        ccTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGac                                                                                                                                                                                     >  1:140776/1‑238 (MQ=255)
                                                              aGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGt                                                                                                                                                                              >  1:1118699/1‑239 (MQ=255)
                                                                     cTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTgcg                                                                                                                                                                                                        <  1:261478/206‑1 (MQ=255)
                                                                               tATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGCTCCTGATGAAAAccgcc                                                                                                                                                             >  1:270037/1‑239 (MQ=255)
                                                                                    gTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGa‑aa                                                                                                                                                        >  1:578290/1‑238 (MQ=255)
                                                                                       aaaTGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGCACCTCGGCGTGCGCCCTAACCCCTTTGTCGACACGCCGTTCCTGATGAAAAACGCCGA‑AAccct                                                                                                                                                    >  2:386638/1‑237 (MQ=255)
                                                                                       aaaTGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCAt                                                                                                                                                    >  1:4159/1‑239 (MQ=255)
                                                                                                   acgctGCGGTTGCGC‑TCCCCCAGGAGCGCGCGTTTTTCCGCCGGTTTCCCGACCGGCTATACCGCGCTGCTCGGCGGGTTAACCGAGACCGGACGGAGCGCGGTAACC‑T‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTACCAAAGCGTGCAATTCCCGCACCCGACCCTCGGCGCGCCCACTAACGCTTTGATCGCCACGCGGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTAc                                                                                                                                        <  2:270037/235‑1 (MQ=255)
                                                                                                   accGTGCTGTT‑CGCGTCCCCCAGTGGGGCTCTGTATTCCTCCGCTTCCCCGCCCGGGTATCCCGCCCGGCTCGGCGGGTT‑ACCCAAAACCGACCGTGCCGGGTGAACGG‑T‑ACTTTC‑TTCCTTTGCCCTTGACCCCAGTCAAAGCGTGAAATTCCCACACCCAAGCCCCGCCGTCCCCACTACCGCATGGTTCGCCACGCCGTTCCTGATGAAAACCGCCGA‑AACCACTCGCTTCGGTAc                                                                                                                                        <  2:779943/237‑1 (MQ=255)
                                                                                                     cGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCATGTTGAAAACCGCCGA‑CACCATTCGCTTCGGTACCg                                                                                                                                      >  1:1194119/1‑239 (MQ=255)
                                                                                                       tGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGt                                                                                                                                    >  1:1077410/1‑239 (MQ=255)
                                                                                                                     caccaGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCt                                                                                                                       >  2:288355/1‑239 (MQ=255)
                                                                                                                          gtgccgggctgtattcggCCGGTTGCGCACCCGGTGATCCCGCAGTGCGCGGCGGTGTAACGGAAAACGGAGGTGGCGCGGTGACC‑T‑G‑TCTTCCGTTCCTTTC‑CGTTGCCGCTTAGCAAGCCGTTCAATTCCCCCAACCGAACCTCGCCGGGCGCAAAAACGCCTTTCCCGACACGCCTGTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTaa                                                                                                                   <  2:165096/221‑1 (MQ=255)
                                                                                                                               gcgcgTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGa                                                                                                             >  1:188933/1‑239 (MQ=255)
                                                                                                                               gcgcgTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGATCCTGATGAAAACCGCCGG‑AACCATTCGCGTCGGTACCGGCATTCCGCAAATCTTTAACGCTGa                                                                                                             >  1:769894/1‑239 (MQ=255)
                                                                                                                               gcgcgTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAAAGCCTTGATCGACACGCCGTTCCTGATGAAAAACCCCGA‑AACCATTCGCTTCGGGACCGGGATTACGCCAATCTTTAACGGTGa                                                                                                             >  2:265141/1‑239 (MQ=255)
                                                                                                                               gcgcgTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGGGCAATTACCGCAACCGAACCTCGGCGTGCGCCCTAACGCCCTTATCGACACCGCGTTCCTAATCAAAACCGCCCACAACCA‑TCGCCTCGGGCCCGGGATTCCGCAAATCTTTAACgggag                                                                                                             >  2:134176/1‑235 (MQ=255)
                                                                                                                               gcgcgTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTACAAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCAATACCGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATg                                                                                                              <  2:356071/238‑1 (MQ=255)
                                                                                                                               gcgcgTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGCAGCGCGGTGCAC‑G‑T‑GCGTTCGTTCCTTTG‑CCTTGACGCCCTTCAAAGCGTGCAATTACCGCAACCCAACCTCGGCCTGCCCCCTAACACCTTGACCCGTCCGCCCTTCCTGATACAAACCGCCGA‑AACCACTCACTTCCGGACCGCGTTTCCGCAAATCTTTAACGATGa                                                                                                             >  2:818354/1‑239 (MQ=255)
                                                                                                                                cgcgTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGaa                                                                                                            >  1:661218/1‑239 (MQ=255)
                                                                                                                                cgcgTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGaa                                                                                                            >  1:165096/1‑239 (MQ=255)
                                                                                                                                  cgTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCGTGATCGACCAGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAgt                                                                                                          >  1:197373/1‑239 (MQ=255)
                                                                                                                                     tATTTCCCGGGTTCCCCGACCGGCTATACCGCACTGCTCGGCGGGTTACCCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTCCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGAAATTACCGCAACCGAACCCCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAgtggt                                                                                                       <  2:1094681/239‑1 (MQ=255)
                                                                                                                                            ccGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGGGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGC‑AACCATTCGCCTCGGTACCGGTATTCCGCAAATATTTAACGATGAAgtg                                                                                                         >  1:618728/1‑230 (MQ=255)
                                                                                                                                                tttCCCGACCGGCTATACCCCAGTGCTCGGCGGGTTACCCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCTTTCCTTTG‑CCTTGACGCCTACAAAAGCGTGCAATTCCCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCg                                                                                             <  1:958462/238‑1 (MQ=255)
                                                                                                                                                       aCCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCa                                                                                     >  1:185189/1‑238 (MQ=255)
                                                                                                                                                          ggCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGAGACGTTAAA‑T‑T‑GCTTTGGTTCCTTTG‑CCTTGACGCCTATCAAAGAGTGCAATTACCGCAACCGAACCTCGGCGGGCGCACTAACGCCATTATCGAACCGCCCTTCCTGATGAAAACCGGCGA‑AACCCATCGCTTCGGCACCGGTAATCCGTAAATCTTTAAAGATGAAGCGGCGGTGCCAGCCTTC‑CTCaaac                                                                                  >  2:1028997/1‑236 (MQ=255)
                                                                                                                                                          ggCTATAACGCACTGCTCGGCGGGTTAAACGAGAACGGGCGTAGCGCGGAGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGAGCAATTACCGCAACCGAACCTCGGCGCGCCCCTTAACGCCTTGAGCGACACGCCGTTCCTGCTGAAAACCGCCGA‑AACCATTCGCTTCGCTACCGGTATTCCTCCAATCATAAACGATGAAGAGGTGGTGCCAGCGTTG‑CTCATCCg                                                                                 >  2:430498/1‑239 (MQ=255)
                                                                                                                                                                 ccGCCCTGCCCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTGTGGTTCCTTTG‑CCTTGACGCCTATAAAAGCGTGCAATTCCCGCAACCGAACCTCGGCGTGCGCCCTAACGCCTTGATCGCCACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGtt                                                                          <  2:874974/239‑1 (MQ=255)
                                                                                                                                                                 ccGCACCGCCCGGCGGGTTAGCCGAGAACGGCCGAGGCGCGGTGACC‑G‑T‑GCTCGCGTACGTCTT‑CTCTGGCGCCCATCACGGCGTACATTTGCCGCACCCGTACTCCGCGGCGCGCCCAACCGCATGCATCAAGACCCGGTTCCCGAAGAAAGCCGCCGA‑AACCATTCGCGTCGGTACCGGTATTCCGCAAATCTTTAACGATGAGGTGGTGGTGGCAGCGTTC‑CTCACCCGTGGCGtt                                                                          <  2:541071/239‑1 (MQ=255)
                                                                                                                                                                    cACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCtt                                                                                                                      >  2:284880/1‑193 (MQ=255)
                                                                                                                                                                     aCTGCTCGGCGGGTTAACCGAGAAAGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCTAACCTCGGCGGGCGCACTAACGCCTTGATCGACACGCCGTTCCTTATGAAAACCGCCGG‑AACCATTCCCTTCGGTACCGGGATTCCGCAAATATTTAACGATGACGTGGTGGTGGCACCGTTC‑CTCTACCGTGTCTTTTc                                                                        >  2:546463/1‑237 (MQ=255)
                                                                                                                                                                      cTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGGGCCAGCGTTC‑CTCAACCGTGGCGTTTc                                                                        >  1:273520/1‑236 (MQ=255)
                                                                                                                                                                       tGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGACGTGGTGGTTCCAGCGTTC‑CTCAACCGGGGCGTTTCGCTg                                                                    >  2:151214/1‑239 (MQ=255)
                                                                                                                                                                       tGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCCATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCCACACGCCGTTCCCGATGAAAACCGCCGA‑AACCATTCGCTTCGGTTCCGGTATTCCGCAAATCTTTAAACAAGAAGATGTGGTTCCAGAGCTCACTCAA‑CGAGGCGTTTctctc                                                                    >  2:1093041/1‑238 (MQ=255)
                                                                                                                                                                         cTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTT‑CCTTGACGCCTATCAAAGCGGGCAATTACCGCAAACGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCCTTCGCTTCGGTACCGGTATTCCGCCAATCTTTAACCATGACATGGCGGTGCCACCGTTC‑CTCCACCCTGGCGTTTCGCttgc                                                                  >  2:487017/1‑236 (MQ=255)
                                                                                                                                                                         cTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGGAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGGTGTGCCAGCGTTC‑CCCAACCGTGGCGTTTCGCTGGa                                                                  >  2:506482/1‑239 (MQ=255)
                                                                                                                                                                         cTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGa                                                                  >  1:75009/1‑239 (MQ=255)
                                                                                                                                                                         cTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTAACGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGa                                                                  >  1:1004555/1‑239 (MQ=255)
                                                                                                                                                                         cTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTACCAAAGCGTGCAATTACCGCAACCGAACCTCTGCGTGCGCACTAACGCCTTGCGCGACCCGCCGTTCCTGATGAAACCCGCCGG‑AACCATTCGCTTCGGTACCGCTAGTCCGCAAAACTTTAACGATGACGTGGTGGCGACAGCGGTC‑GCCAACCGGGGCGTTTCGcgtgg                                                                  >  2:227456/1‑235 (MQ=255)
                                                                                                                                                                              cggGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTc                                                                                                                                                                            >  1:789146/1‑130 (MQ=255)
                                                                                                                                                                                 gTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATgcgc                                                          >  2:297736/1‑239 (MQ=255)
                                                                                                                                                                                     ccaggaaaCGGATGTGGGGCGGGGAAC‑T‑G‑CCTTTCGTTCGTTTG‑CCTGGACGCCTAACAAAGCGTCCAATTCCCGCAACCGAACCCGGGCGGGCGCAAAAACGCTTTGGTGGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGa                                                      <  2:313685/233‑1 (MQ=255)
                                                                                                                                                                                     aCCGAGCACGGACGTGGCGCGGTGACC‑G‑G‑ACTTATGTTCCTTTG‑CCTGGACGCATATCAATGCGAGCAATTCCCGAAACGGAACCTTGGCGGGCGCACTAACGCCTTGACCGCAACGCCTTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGTCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGa                                                      <  2:940035/239‑1 (MQ=255)
                                                                                                                                                                                      ccgagagatggaggtgcggGGTGGAC‑G‑TCGCTTTGTTTCTTGTG‑CCTGGCGCCCTATCAATCGTTGCAATTACGGCACCCAACCCTGCGCGTGCGCACTAACGCTTTTATCGAGACGCGGTTCCTGATAAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGa                                                      <  2:34717/224‑1 (MQ=255)
                                                                                                                                                                                          aaagggcggggggggggTGAAC‑T‑G‑CCTTCCGTCCTTTTG‑CCTTGCCGCCTATCAAGGCTAGCAATTACCGCACCCGACCCCCGGCGGGCGCATAAACGCCTTGATGGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTAtt                                                 <  2:6228/224‑1 (MQ=255)
                                                                                                                                                                                           aaCGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGGGCGCGACTAtt                                                 >  1:511738/1‑238 (MQ=255)
                                                                                                                                                                                            ccGGCCGGAGCGCGGTGACC‑C‑T‑GCTTCCGTTCCTTTG‑CTTGTCCCCCTCTAAAGGCGTGCAATTACCGCACCCGAACCCGGGCGTGCCCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTcc                                               <  2:941782/238‑1 (MQ=255)
                                                                                                                                                                                            aCGGCCGTGGCGGGGT‑AAC‑GTT‑GCTTTCGTTCCTTCG‑CGTTGACCCTTACAAAAGGGTGCAATTCCCGCAACCGACCCTGGGCTTGCCCCCAAACGCCTGGACCGCAACGCCGTTCCTGATAAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTAtt                                                 <  2:257950/237‑1 (MQ=255)
                                                                                                                                                                                                aCGTCGCGGGGTGAAC‑G‑T‑GCTTTCGTTCCTTGC‑CCTTGACCCCTATCAAGGCGTGCAATTGCCGCAACCGAACCCGGGCGTGCGCACTAACGCTTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTa                                            <  1:299791/238‑1 (MQ=255)
                                                                                                                                                                                                    accgcgTTGAAC‑G‑T‑TCTTTCGTTCCTTTG‑CCTGGACGCCTACCAAAGCGTCCAATTACCGCACCCGACCCCCGGCGTGCGCACTACCGCTTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTggg                                       <  1:660126/237‑1 (MQ=255)
                                                                                                                                                                                                    aGCGCGGTGACC‑G‑T‑GCTTTCTTTCCTTGG‑CCTTGCCGCCTATCAAAGCGTGCAATTCCCGCAACCGACCCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTggg                                       <  1:151214/239‑1 (MQ=255)
                                                                                                                                                                                                    aGCGCGGGGAAC‑T‑T‑GCTTTCGTCCCTTTG‑CCTGGACGCCATACAAGGCGTGCAATTCCCGCACCCGAACCGCGCCGTGCCCACTACCGCCTTGATCGCCACGCCGTTCCTGATAAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTggg                                       <  2:995037/239‑1 (MQ=255)
                                                                                                                                                                                                     gcgggttGAAC‑G‑T‑GCTTTCTTCCCTTTG‑CCTTGACCCCTACCAAAGCGTGCATTTCCCGCACCCGACCCCGGGCGTGCGCACTAACCCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTggg                                       <  1:284201/232‑1 (MQ=255)
                                                                                                                                                                                                         ggTGAAC‑G‑T‑GCTTTCGTTCTTTTG‑CCTTGCCGCCTATCAACGCGTGCAATTCCCACACCCGACCCCCGGCGTGCCCACTAACCCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCtgtg                                  <  1:714027/239‑1 (MQ=255)
                                                                                                                                                                                                          gTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGAGACTATTCCGTAGTGGGCtgtgt                                 >  1:595365/1‑239 (MQ=255)
                                                                                                                                                                                                             gtcaacggtgtatctttCCTGTG‑CCTTGACCCCTATCAAAGCGTAAAATTACCGCAACCGAACCTCGGCGGGAGCCCTACCACTGTGATCGACACGCCGTTTCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCtgtg                                  <  2:203475/223‑1 (MQ=255)
                                                                                                                                                                                                                          cGTTCTTTGG‑CCTTGACCCCTAACAAAGCGGGCATTTACCGCAACCGACCCTCGGCGTGCGCACTACCGCTTTGTGCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTAtt                    <  2:1118699/239‑1 (MQ=255)
                                                                                                                                                                                                                             tcttttG‑CCTTGACGCCTACCAAAGCGTGCAATTCCCGAAACCGACCCTCGGCGTCCGCCCTAACGCTTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTAGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTcc                  <  1:244806/235‑1 (MQ=255)
                                                                                                                                                                                                                                 tgg‑CCTTGACGCCATCCAAAGCGCGCATTTCCCGCACCCGAACCGCGGCGTGCCCACTAACGCCTTGAGCGACACCCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCa             <  2:661218/237‑1 (MQ=255)
                                                                                                                                                                                                                                  tG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAg            >  1:986776/1‑239 (MQ=255)
                                                                                                                                                                                                                                           cGCTTACCAAACCGTGCAATTCCCGAACCCGACCCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACg    <  1:388270/239‑1 (MQ=255)
                                                                                                                                                                                                                                           cGCCTATCAAAGCGTGCAATTCCCGCAACCGACCCTCGGCTTGCCCCCTAACGCCTGGATCGCCACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACg    <  1:1009671/239‑1 (MQ=255)
                                                                                                                                                                                                                                             ccTATCAAAGCGTGCACTTCCCGCAACCGACCCTCGGCGTCCGCCCTAACGCCTTGATCGACACCCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGc  <  1:1205975/239‑1 (MQ=255)
                                                                                                                                                                                                                                               tATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTggg                                       >  1:255111/1‑200 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                            
CAGTATATGTTGCCGTTCTATCAGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGC‑TCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAAC‑G‑T‑GCTTTCGTTCCTTTG‑CCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGA‑AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTC‑CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGC  >  NC_000913/4144813‑4145283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑