Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,145,064 A→C 17.5% L357F (TTA→TTC)  pflD → putative glycine radical domain‑containing pyruvate formate‑lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,145,0640AC17.5% 354.4 / 5.9 156L357F (TTA→TTC) pflDputative glycine radical domain‑containing pyruvate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base A (83/33);  new base C (3/34);  total (87/69)
Fisher's exact test for biased strand distribution p-value = 3.51e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 156 total aligned reads displayed.

GTTCTATCAGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCG  >  NC_000913/4144827‑4145298
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                          
gTTCTATCAGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTAc                                                                                                                                                                                                                                           <  2:473298/239‑1 (MQ=255)
gTTCTATCAGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTAc                                                                                                                                                                                                                                           <  1:713962/239‑1 (MQ=255)
   cTATCAGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAACTGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGACCGGACGTAGCGCGGTGAAAG‑T‑GCTTTCGTTCCTATGCCTTGTAGCCTATCAAA‑ACGTGCAATTCCCg                                                                                                                                                                                                                                         >  2:774072/1‑238 (MQ=255)
      tCAGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCCATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGGAGCGCGGTGAACG‑T‑GCTTTCGTTCCTATGCCGTGACGCCTATCAAA‑GCGGGCACTTACCGCaa                                                                                                                                                                                                                                      >  2:1159084/1‑238 (MQ=255)
       cAGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAAcc                                                                                                                                                                                                                                    <  1:965796/239‑1 (MQ=255)
        aGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGGCGCCTATCAAC‑GAGTGCAATTACCGCAAcc                                                                                                                                                                                                                                    >  2:1128298/1‑238 (MQ=255)
            atcatTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGGAGCGCCGTGAACG‑T‑GCTTTCGTTCCTTTTCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGaa                                                                                                                                                                                                                                 >  1:1038964/1‑237 (MQ=255)
              catTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTc                                                                                                                                                                                                                             <  1:412970/239‑1 (MQ=255)
                ttAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCg                                                                                                                                                                                                                            >  1:1062492/1‑238 (MQ=255)
                ttAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCg                                                                                                                                                                                                                            >  1:905314/1‑238 (MQ=255)
                ttAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATAACGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCCGGGCGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTCCCGCAACCGAACATCg                                                                                                                                                                                                                            >  1:907441/1‑238 (MQ=255)
                 tAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGc                                                                                                                                                                                                                          <  1:632403/239‑1 (MQ=255)
                        gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGACCG‑T‑GCTTTCGTTCCTTTGCCTTCACGCCTATCACA‑GCGTGGCATTCCCGCACCCGAACCCCGGCGTgcgc                                                                                                                                                                                                                    >  2:852206/1‑238 (MQ=255)
                        gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCg                                                                                                                                                                                                                                         <  1:342676/217‑1 (MQ=255)
                        gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAAcc                                                                                                                                                                                                                                    <  2:397641/222‑1 (MQ=255)
                        gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGACACCGAACCTCGGCGTgcgc                                                                                                                                                                                                                    >  1:135082/1‑238 (MQ=255)
                        gggCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGCAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTGTCAAA‑GCCTGCAATTACGGCAACCGAACCTCCCCGTgcgc                                                                                                                                                                                                                    >  2:1205975/1‑238 (MQ=255)
                          gCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCCCCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGCCGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGGGTGCGCACt                                                                                                                                                                                                                 >  1:479167/1‑239 (MQ=255)
                           cGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACt                                                                                                                                                                                                                 <  1:159532/238‑1 (MQ=255)
                           cGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACt                                                                                                                                                                                                                 <  1:737940/238‑1 (MQ=255)
                             gtctgatagaacggcaacatataCTGGTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑G‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCACTTACCGCAACCGAACCTCGGCGTGCGCACTAAc                                                                                                                                                                                                              >  1:20900/23‑239 (MQ=255)
                                   cggcgTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGGGCCATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTg                                                                                                                                                                                                        >  1:828852/1‑239 (MQ=255)
                                          ccTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGac                                                                                                                                                                                                  >  1:140776/1‑238 (MQ=255)
                                          ccTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGac                                                                                                                                                                                                  >  1:255511/1‑238 (MQ=255)
                                                aGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGt                                                                                                                                                                                           >  1:1118699/1‑239 (MQ=255)
                                                       cTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCCTTCCtgat                                                                                                                                                                                    >  1:1071362/1‑239 (MQ=255)
                                                                 tATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGCTCCTGATGAAAAccgcc                                                                                                                                                                          >  1:270037/1‑239 (MQ=255)
                                                                      gTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGaaa                                                                                                                                                                      >  1:578290/1‑238 (MQ=255)
                                                                         aaaTGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGCACCTCGGCGTGCGCCCTAACCCCTTTGTCGACACGCCGTTCCTGATGAAAAACGCCGAAAccct                                                                                                                                                                  >  2:386638/1‑237 (MQ=255)
                                                                                  gtcagtgggcttgtGCGCACCCCCAGGAGCGCGGTTTATTTCGCCGGTTCCCCGA‑CCGGCTATTCCCCACTGCTTGGCTGGTTACCCGAGAACGGACGTAGCGTGGTGAACG‑T‑GCTTCCGTTCCTTTCCCTTGACGCCTTTCAAT‑GCGTCCACTTCCCGCACCCGAACCTCGCGGTGCCCACTACCTCTTTGATCGACCCGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCgg                                                                                                                                                         <  2:206547/226‑1 (MQ=255)
                                                                                     aTCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATCACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACCCGCCGATCCTGATGCAAACCGCCGAAACCATTCGCTTCGGTa                                                                                                                                                       >  1:219539/1‑238 (MQ=255)
                                                                                       cGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCATGTTGAAAACCGCCGACACCATTCGCTTCGGTACCg                                                                                                                                                    >  1:1194119/1‑239 (MQ=255)
                                                                                       cGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGCTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGGGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTACCTTCGGTACCg                                                                                                                                                    >  1:923594/1‑239 (MQ=255)
                                                                                         tGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGt                                                                                                                                                  >  1:1077410/1‑239 (MQ=255)
                                                                                                        ccaGTGGCGCGTGTTTTTTC‑CCGGTTTCCCCACCCGGCTCTCCCGCCCCGCTCGGCGGTTTACCCCAGACCGGACGTAGCGCGGTGAACG‑T‑GCTTGCGTTCCTTTGCCTGGACCCCTACAAAA‑GCTTGCATTTCCCGCAACCGACCCTCGGCGGGCCCACAACCGCTTGTTTCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCttt                                                                                                                                   <  2:1071362/239‑1 (MQ=255)
                                                                                                        ccaGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAAc                                                                                                                                                                                                                                     <  1:1105470/141‑1 (MQ=255)
                                                                                                        ccaGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAAc                                                                                                                                                                                                                                     >  2:1105470/1‑141 (MQ=255)
                                                                                                             aGCGCGCGTTATTCCGCCGGTTTCCCGA‑CCGGCTATCCCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCACCCGACCCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGa                                                                                                                              <  1:196885/239‑1 (MQ=255)
                                                                                                                gcgcgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACCCGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGAttg                                                                                                                           >  1:267655/1‑237 (MQ=255)
                                                                                                                gcgcgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGATCCTGATGAAAACCGCCGGAACCATTCGCGTCGGTACCGGCATTCCGCAAATCTTTAACGCTGa                                                                                                                           >  1:769894/1‑239 (MQ=255)
                                                                                                                gcgcgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAAAGCCTTGATCGACACGCCGTTCCTGATGAAAAACCCCGAAACCATTCGCTTCGGGACCGGGATTACGCCAATCTTTAACGGTGa                                                                                                                           >  2:265141/1‑239 (MQ=255)
                                                                                                                gcgcgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTACAAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCAATACCGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATg                                                                                                                            <  2:356071/238‑1 (MQ=255)
                                                                                                                gcgcgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGCAGCGCGGTGCACG‑T‑GCGTTCGTTCCTTTGCCTTGACGCCCTTCAAA‑GCGTGCAATTACCGCAACCCAACCTCGGCCTGCCCCCTAACACCTTGACCCGTCCGCCCTTCCTGATACAAACCGCCGAAACCACTCACTTCCGGACCGCGTTTCCGCAAATCTTTAACGATGa                                                                                                                           >  2:818354/1‑239 (MQ=255)
                                                                                                                acgcgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCCTGGAATTACCGCCACCGAAACCCGACAGGCGCACTAACGCCCTGATCGACACGCCGTTCCCGATGAAACCCCCCGCAACCATTCACTTCTGTCCCGGCATTCCCCCCATCTTTAACGAtca                                                                                                                           >  2:171263/2‑237 (MQ=255)
                                                                                                                 cgcgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGaa                                                                                                                          >  1:165096/1‑239 (MQ=255)
                                                                                                                 cgcgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGaa                                                                                                                          >  1:661218/1‑239 (MQ=255)
                                                                                                                   cgTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCGTGATCGACCAGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAgt                                                                                                                        >  1:197373/1‑239 (MQ=255)
                                                                                                                      tATTTCCCGGGTTCCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTACCCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTCCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGAAATTACCGCAACCGAACCCCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAgtggt                                                                                                                     <  2:1094681/239‑1 (MQ=255)
                                                                                                                             ccGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGGGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGCAACCATTCGCCTCGGTACCGGTATTCCGCAAATATTTAACGATGAAgtg                                                                                                                       >  1:618728/1‑230 (MQ=255)
                                                                                                                                 tttCCCGA‑CCGGCTATACCCCAGTGCTCGGCGGGTTACCCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCTTTCCTTTGCCTTGACGCCTACAAAA‑GCGTGCAATTCCCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCg                                                                                                           <  1:958462/238‑1 (MQ=255)
                                                                                                                                        a‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCCTTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCCGCGTTCCTCa                                                                                                    >  1:34717/1‑238 (MQ=255)
                                                                                                                                        a‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCa                                                                                                    >  1:185189/1‑238 (MQ=255)
                                                                                                                                            ggCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGAGACGTTAAAT‑T‑GCTTTGGTTCCTTTGCCTTGACGCCTATCAAA‑GAGTGCAATTACCGCAACCGAACCTCGGCGGGCGCACTAACGCCATTATCGAACCGCCCTTCCTGATGAAAACCGGCGAAACCCATCGCTTCGGCACCGGTAATCCGTAAATCTTTAAAGATGAAGCGGCGGTGCCAGCCTTCCTCaaac                                                                                                 >  2:1028997/1‑236 (MQ=255)
                                                                                                                                            ggCTATAACGCACTGCTCGGCGGGTTAAACGAGAACGGGCGTAGCGCGGAGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGAGCAATTACCGCAACCGAACCTCGGCGCGCCCCTTAACGCCTTGAGCGACACGCCGTTCCTGCTGAAAACCGCCGAAACCATTCGCTTCGCTACCGGTATTCCTCCAATCATAAACGATGAAGAGGTGGTGCCAGCGTTGCTCATCCg                                                                                                >  2:430498/1‑239 (MQ=255)
                                                                                                                                                   ccGCCCTGCCCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTGTGGTTCCTTTGCCTTGACGCCTATAAAA‑GCGTGCAATTCCCGCAACCGAACCTCGGCGTGCGCCCTAACGCCTTGATCGCCACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGtt                                                                                         <  2:874974/239‑1 (MQ=255)
                                                                                                                                                   ccGCACCGCCCGGCGGGTTAGCCGAGAACGGCCGAGGCGCGGTGACCG‑T‑GCTCGCGTACGTCTTCTCTGGCGCCCATCACG‑GCGTACATTTGCCGCACCCGTACTCCGCGGCGCGCCCAACCGCATGCATCAAGACCCGGTTCCCGAAGAAAGCCGCCGAAACCATTCGCGTCGGTACCGGTATTCCGCAAATCTTTAACGATGAGGTGGTGGTGGCAGCGTTCCTCACCCGTGGCGtt                                                                                         <  2:541071/239‑1 (MQ=255)
                                                                                                                                                      cACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCtt                                                                                                                                    >  2:284880/1‑193 (MQ=255)
                                                                                                                                                      cACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATAAAA‑GCGTGAAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCtt                                                                                                                                    <  1:284880/193‑1 (MQ=255)
                                                                                                                                                       aCTGCTCGGCGGGTTAACCGAGAAAGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCTAACCTCGGCGGGCGCACTAACGCCTTGATCGACACGCCGTTCCTTATGAAAACCGCCGGAACCATTCCCTTCGGTACCGGGATTCCGCAAATATTTAACGATGACGTGGTGGTGGCACCGTTCCTCTACCGTGTCTTTTc                                                                                       >  2:546463/1‑237 (MQ=255)
                                                                                                                                                         tGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGACGTGGTGGTTCCAGCGTTCCTCAACCGGGGCGTTTCGCTg                                                                                   >  2:151214/1‑239 (MQ=255)
                                                                                                                                                           cTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTTCCTTGACGCCTATCAAA‑GCGGGCAATTACCGCAAACGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCCTTCGCTTCGGTACCGGTATTCCGCCAATCTTTAACCATGACATGGCGGTGCCACCGTTCCTCCACCCTGGCGTTTCGCttgc                                                                                 >  2:487017/1‑236 (MQ=255)
                                                                                                                                                           cTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGGAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGGTGTGCCAGCGTTCCCCAACCGTGGCGTTTCGCTGGa                                                                                 >  2:506482/1‑239 (MQ=255)
                                                                                                                                                           cTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGa                                                                                 >  1:75009/1‑239 (MQ=255)
                                                                                                                                                           cTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTAACGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGa                                                                                 >  1:1004555/1‑239 (MQ=255)
                                                                                                                                                           cTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTACCAAA‑GCGTGCAATTACCGCAACCGAACCTCTGCGTGCGCACTAACGCCTTGCGCGACCCGCCGTTCCTGATGAAACCCGCCGGAACCATTCGCTTCGGTACCGCTAGTCCGCAAAACTTTAACGATGACGTGGTGGCGACAGCGGTCGCCAACCGGGGCGTTTCGcgtgg                                                                                 >  2:227456/1‑235 (MQ=255)
                                                                                                                                                                cggGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTc                                                                                                                                                                                         >  1:789146/1‑130 (MQ=255)
                                                                                                                                                                cggGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTc                                                                                                                                                                                         <  2:789146/130‑1 (MQ=255)
                                                                                                                                                                   gTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATgcgc                                                                         >  2:297736/1‑239 (MQ=255)
                                                                                                                                                                     tatgcGAGAGCGGACGTAGCAGGGTGAACG‑T‑GCTTTCGTTCCTGTTCCGTGACGCCTCCAAAA‑GCGTGCAATTACGCCACCCGAACCTCGGCTCGCCCACTACCGCCTTGATCGACCCGCCGTTCCTGAATAAACCCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATgcgcgc                                                                       <  2:147355/235‑1 (MQ=255)
                                                                                                                                                                       ccaggaaaCGGATGTGGGGCGGGGAACT‑G‑CCTTTCGTTCGTTTGCCTGGACGCCTAACAAA‑GCGTCCAATTCCCGCAACCGAACCCGGGCGGGCGCAAAAACGCTTTGGTGGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGa                                                                     <  2:313685/233‑1 (MQ=255)
                                                                                                                                                                       aCCGAGCACGGACGTGGCGCGGTGACCG‑G‑ACTTATGTTCCTTTGCCTGGACGCATATCAAT‑GCGAGCAATTCCCGAAACGGAACCTTGGCGGGCGCACTAACGCCTTGACCGCAACGCCTTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGTCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGa                                                                     <  2:940035/239‑1 (MQ=255)
                                                                                                                                                                        ccgagagatggaggtgcggGGTGGACG‑TCGCTTTGTTTCTTGTGCCTGGCGCCCTATCAAT‑CGTTGCAATTACGGCACCCAACCCTGCGCGTGCGCACTAACGCTTTTATCGAGACGCGGTTCCTGATAAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGa                                                                     <  2:34717/224‑1 (MQ=255)
                                                                                                                                                                            aaagggcggggggggggTGAACT‑G‑CCTTCCGTCCTTTTGCCTTGCCGCCTATCAAG‑GCTAGCAATTACCGCACCCGACCCCCGGCGGGCGCATAAACGCCTTGATGGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTAtt                                                                <  2:6228/224‑1 (MQ=255)
                                                                                                                                                                             aaCGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGGGCGCGACTAtt                                                                >  1:511738/1‑238 (MQ=255)
                                                                                                                                                                              ccGGCCGGAGCGCGGTGACCC‑T‑GCTTCCGTTCCTTTGCTTGTCCCCCTCTAAAG‑GCGTGCAATTACCGCACCCGAACCCGGGCGTGCCCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTcc                                                              <  2:941782/238‑1 (MQ=255)
                                                                                                                                                                              aCGGCCGTGGCGGGGT‑AACGTT‑GCTTTCGTTCCTTCGCGTTGACCCTTACAAAA‑GGGTGCAATTCCCGCAACCGACCCTGGGCTTGCCCCCAAACGCCTGGACCGCAACGCCGTTCCTGATAAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTAtt                                                                <  2:257950/237‑1 (MQ=255)
                                                                                                                                                                               acgggacggacggagtggacctggCTGTCGTTCC‑TTCCCTTGACGCCTTACAAAGGCTGGCATTTCCCGCACCCGAACCTGGGCGCCGCCACAACCCCCTGGAGCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTAtt                                                                <  2:287656/215‑1 (MQ=255)
                                                                                                                                                                                 gACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGCTGCGCGCGACTATTCCGTa                                                           >  1:995037/1‑239 (MQ=255)
                                                                                                                                                                                  aCGTCGCGGGGTGAACG‑T‑GCTTTCGTTCCTTGCCCTTGACCCCTATCAAG‑GCGTGCAATTGCCGCAACCGAACCCGGGCGTGCGCACTAACGCTTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTa                                                           <  1:299791/238‑1 (MQ=255)
                                                                                                                                                                                  aCGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGACCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTa                                                           <  1:230425/238‑1 (MQ=255)
                                                                                                                                                                                      cgcgcGTTGAACG‑T‑GTTTTCGTTCCTTTCCCTTGACGCCTACAAAA‑CCTTGCAATTCCCGCACCCGACCCCCGGCGGGCGCACTACCGCCTTGTTCGACACGCCGTTCCTGAGGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTggg                                                      <  1:1093041/238‑1 (MQ=255)
                                                                                                                                                                                      aGCGCGGTGACCG‑T‑GCTTTCTTTCCTTGGCCTTGCCGCCTATCAAA‑GCGTGCAATTCCCGCAACCGACCCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTggg                                                      <  1:151214/239‑1 (MQ=255)
                                                                                                                                                                                      aGCGCGGGGAACT‑T‑GCTTTCGTCCCTTTGCCTGGACGCCATACAAG‑GCGTGCAATTCCCGCACCCGAACCGCGCCGTGCCCACTACCGCCTTGATCGCCACGCCGTTCCTGATAAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTggg                                                      <  2:995037/239‑1 (MQ=255)
                                                                                                                                                                                       gcgggttGAACG‑T‑GCTTTCTTCCCTTTGCCTTGACCCCTACCAAA‑GCGTGCATTTCCCGCACCCGACCCCGGGCGTGCGCACTAACCCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTggg                                                      <  1:284201/232‑1 (MQ=255)
                                                                                                                                                                                           ggTGAACG‑T‑GCTTTCGTTCTTTTGCCTTGCCGCCTATCAAC‑GCGTGCAATTCCCACACCCGACCCCCGGCGTGCCCACTAACCCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCtgtg                                                 <  1:714027/239‑1 (MQ=255)
                                                                                                                                                                                                       ctttgcttCTTTCGCCTTGGCGCCTATCAAA‑GCGGTCAATACCCGCACCCGACCCTCGGCGTGCGCTCTACCGCGTTGATCGACACGCCGTTCCGGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTAt                                        <  2:281918/232‑1 (MQ=255)
                                                                                                                                                                                                           ctgtCCTTTGCCTTGACGCATTACAAC‑GCGTAAAATTACCGCACCCAAACCTCGGCGTGCGCACTAACGCCTTGACCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTAtt                                   <  2:12978/236‑1 (MQ=255)
                                                                                                                                                                                                             ttCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCATCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGCATTATCTCTTc                                  >  1:581999/1‑238 (MQ=255)
                                                                                                                                                                                                              tcttttGCCTTGACGCCTACCAAA‑GCGTGCAATTCCCGAAACCGACCCTCGGCGTCCGCCCTAACGCTTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTAGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTcc                                 <  1:244806/235‑1 (MQ=255)
                                                                                                                                                                                                                  tggCCTTGACGCCATCCAAA‑GCGCGCATTTCCCGCACCCGAACCGCGGCGTGCCCACTAACGCCTTGAGCGACACCCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCa                            <  2:661218/237‑1 (MQ=255)
                                                                                                                                                                                                                           cGCCTATCAAA‑GCGTGCAATTCCCGCAACCGACCCTCGGCTTGCCCCCTAACGCCTGGATCGCCACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACg                   <  1:1009671/239‑1 (MQ=255)
                                                                                                                                                                                                                            cgccatcacaggggTGCAATTCCCGCACCCGACCCTCGGCTCGCGCACAACCGCCTGTATCGACCCGCCTTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACg                   <  2:578290/226‑1 (MQ=255)
                                                                                                                                                                                                                             ccTATCAAA‑GCGTGCACTTCCCGCAACCGACCCTCGGCGTCCGCCCTAACGCCTTGATCGACACCCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGc                 <  1:1205975/239‑1 (MQ=255)
                                                                                                                                                                                                                                  cAAA‑GCGTGCAATTACCGCACCCGAACCCCGGCGTCCGCACTAACGCCTTGACCGACACGCCGTTCCTTATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCa            <  1:150863/239‑1 (MQ=255)
                                                                                                                                                                                                                                    aa‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTg              >  2:21199/1‑235 (MQ=255)
                                                                                                                                                                                                                                         gTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACa       >  1:401393/1‑238 (MQ=255)
                                                                                                                                                                                                                                          tGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATc     >  1:397366/1‑239 (MQ=255)
                                                                                                                                                                                                                                          tGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGGGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGGATGACATc     >  1:965147/1‑239 (MQ=255)
                                                                                                                                                                                                                                            ctactACCGCCACCACACCTCGTCGTCCCCACGAACGCTTTGAGCGCATCGCCGTCCCTGAAGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTGTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGGGTTTCGCTGTAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGACACTACGGCTTGCATGACATcgc   <  2:267655/235‑1 (MQ=255)
                                                                                                                                                                                                                                             aaTTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATcgcg  <  1:288355/239‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                          
GTTCTATCAGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGA‑CCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACG‑T‑GCTTTCGTTCCTTTGCCTTGACGCCTATCAAA‑GCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCG  >  NC_000913/4144827‑4145298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑