Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,295,833 T→C 4.4% intergenic (+40/+604) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,295,8330TC 4.4% 1117.2 / 25.4 338intergenic (+40/+604)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base T (163/154);  new base C (3/12);  total (168/170)
Fisher's exact test for biased strand distribution p-value = 3.12e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.49e-01

Only 100 of 338 total aligned reads displayed.

CGTGCCTGGCGTGTCGTTTGTGGTGTTGCTGGCAACGCTGGGTAGCGTAGGTATTCCGCTGGAAGGTCTGGCGTTTATTGCTGGTGTTGACCGTATCCTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAA  >  NC_000913/4295547‑4296110
                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                     
cGTGCCTGGCGTGTCGTTTGTGGTGTTGCTGGCAACGCTGGGTAGCGTAGGTATTCCGCTGGAAGGTCTGGCGTTTATTGCTGGTGTTGACCGTATCCTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATg                                                                                                                                                                                                                                                                                      <  2:2004463/288‑1 (MQ=255)
    ccTGGCGTGTCGTTTGTGGTGTTGCTGGCAACGCTGGGTAGCGTAGGTATTCCGCTGGAAGGTCTGGCGTTTATTGCTGGTGTTGACCGTATCCTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATTTGTTAATGCCTGa                                                                                                                                                                                                                                                                                 >  2:1485614/1‑289 (MQ=255)
                 ttGTGGTGTTGCTGGCAACGCTGGGTAGCGTAGGTATTCCGCTGGAAGGTCTGGCGTTTATTGCTGGTGTTGACCGTATCCTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGc                                                                                                                                                                                                                                                                     <  2:2726161/288‑1 (MQ=255)
                            cggataaggcgtttacgccgcatccgacatcaacgcctgatgcgacgcttgccgcgtcttatcaggcctacgccagacagcgcaatagcctgatttagcgtgattttgtaggtcggataaggcgtttatgccgcctccgacatcaacgcctgatgcgacgcttaacgcgtcttatcaggcctacgccagacagcgcaatagcctgctttagcgtgattttgtagttcggataaggcgtttacgccgcatccgacatcAATGACTGCTGCGCCGCTTGCCGCGCCTTa                                                                                                                                                                                                                                                            <  1:2057420‑M1/30‑1 (MQ=255)
                            cggataaggcgtttacgccgcatccgacatcaacgcctgatgcgacgcttgccgcgtcttatcaggcctacgccagacagcgcaatagcctgatttagcgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaacgcctgatgcgacgcttaacgcgtcttatcaggcctacgccagacagcgcaatagcctgatttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcAATGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                                                                                                                                                                                          <  2:2559889‑M1/32‑1 (MQ=255)
                                 aaggcgtttacgccgcgcccgacatgaacgcctgatgcgacgcttgccgcgtcttatcaggccgacgccagacagcgcaatagcctgatttagcgtgattttgtagggcggataaggcgtttatgccgcatccgacatcaacgcctgatgcgacgcttaacgcgtcttatcaggcctacgccagacagcgcaatagcctgatttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                                                                                                                                                                                                     >  1:677907‑M1/253‑289 (MQ=255)
                                         gTAGCGTAGGTATTCCGCTGGAAGGTCTGGCGTTTATTGCTGGTGTTGACCGTATCCTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                                                                                                                                                      <  2:1040849/279‑1 (MQ=255)
                                         gTAGCGTAGGTATTCCGCTGGAAGGTCTGGCGTTTATTGCTGGTGTTGACCGTATCCTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                                                                                                                                                      >  1:1040849/1‑279 (MQ=255)
                                           aGCGTAGGTATTCCGCTGGAAGGTCTGGCGTTTATTGCTGGTGTTGACCGTATCCTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACa                                                                                                                                                                                                                                          <  2:1790607/289‑1 (MQ=255)
                                                  gttttCCGCTGGAAGGTCGTGCGTTTATTGCTGGTGTTGTCCGTATCCTCGCGATGGCGCGTACTGCGCTGAATGTGGTGGGTAATGTGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGCCCGTAATAAAGCGCTGTCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGATGATTGCCGGGGTTATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCaa                                                                                                                                                                                                                                    <  1:2419706/285‑1 (MQ=255)
                                                  gtaagccGCTGGAAGGTGTGGCGTTGATTGCTGGGGTTGACCGTATCCTCGAAATGGCGCGGACTGCGCTGAACGTGATGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATGTGATAAAACTGCGGATCAATAATTGAAGATGGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCaa                                                                                                                                                                                                                                    <  1:1941451/283‑1 (MQ=255)
                                                  gTATTCCGCTGGAAGGTCTGGCGTTTATTGCTGGTGTTGACCGTATCCTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCaa                                                                                                                                                                                                                                    <  1:1787827/288‑1 (MQ=255)
                                                       ccGCTGGAAGGTCTGGCGTTTATTGCTGGTGTTGACCGTATCCTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCGGCGTCTTATGAGTCCTACGCCAGACAGCGCAAtcg                                                                                                                                                                                                                                 >  1:2682503/1‑284 (MQ=255)
                                                       ccGCTGGAAGGTCTGGCGTTTATTGCTGGTGTTGACCGTATCCTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGCAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTATATCAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTCAGCAGGCCCACGACACACAGCGAAATAGctg                                                                                                                                                                                                                              >  1:287428/1‑287 (MQ=255)
                                                           agacaGGTCTGGCGTTGATTGCTAGTGTTGACCGTATCCTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCTCCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGa                                                                                                                                                                                                                            <  2:1104161/283‑1 (MQ=255)
                                                             gAAGGTCTGGCGTTTATTGCTGGTGTTGACCGTATCCTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGcc                                                                                                                                                                                                                                                                                    <  2:844041/229‑1 (MQ=255)
                                                                  tCTGGCGTTTATTGCTGGTGTTGACCGTATCCTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGt                                                                                                                                                                                                                    <  2:1515357/288‑1 (MQ=255)
                                                                    tGGCGTTTATTGCTGGTGTTGACCGTATCCTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGTCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAt                                                                                                                                                                                                                 >  2:1413413/1‑289 (MQ=255)
                                                                            aTTGCTGGTGTTGACCGTATCCTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGCAATGCGCTGGCGGTGCTGGTGATTGCTAAGTGGGAACACAAATTTGACCGTAGGAAAGCGCTGGCTTAGGAGAGTGAAGTGCTGGGCAAATTTGAGCAAACTGCGGATCAATAATTGAAGATTGCCGGGGATAACCACCCGGAAATGTGTGAATGCCTGATGGGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCGAGCGGCAAAGCCGGATCTTGCGTGAtctgtcccg                                                                                                                                                                                                         >  1:1104161/1‑281 (MQ=255)
                                                                             ttGCTGGTGTTGACCGTAGCCTCGGAATGGCGGGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGt                                                                                                                                                                                                        <  1:2290720/289‑1 (MQ=255)
                                                                                cTGGTGTTGACCGTATCCTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGc                                                                                                                                                                                                                                                                     >  2:336558/1‑225 (MQ=255)
                                                                                cTGGTGTTGACCGTATCCTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCaa                                                                                                                                                                                                                                    <  1:1660935/258‑1 (MQ=255)
                                                                                      ttGCCCGTATCCGCGACATGGGGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCGAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGAGGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAgg                                                                                                                                                                                               <  1:1550749/289‑1 (MQ=255)
                                                                                        gACCGTATCCTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGCCTTATCAGGCCTACCCCAGACATCCGAATAGCCTCATCTAGCGTGATTTTGTAGGTCGGATAAGGCg                                                                                                                                                                                             >  1:2032105/1‑289 (MQ=255)
                                                                                           cGTATCCTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGttt                                                                                                                                                                                          >  2:2205524/1‑289 (MQ=255)
                                                                                             tATCCTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                                                                                                                                                      <  2:619386/227‑1 (MQ=255)
                                                                                              aTCCTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATg                                                                                                                                                                                       <  2:668296/289‑1 (MQ=255)
                                                                                                       gacagcgcaatagcctgatttagcgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaacgcctgatgcgacgcttaacgcgtcttatcaggcctacgccagacagcgcaatagcctgatttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                                                                                                                                                                                                     >  1:999056‑M1/183‑219 (MQ=255)
                                                                                                       aTGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCg                                                                                                                                                                              <  2:390653/289‑1 (MQ=255)
                                                                                                       aTGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCg                                                                                                                                                                              >  1:1210220/1‑289 (MQ=255)
                                                                                                         ggCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGAc                                                                                                                                                                            >  2:1245126/1‑289 (MQ=255)
                                                                                                           cgcgTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                                                                                                                                                      <  1:1479230/213‑1 (MQ=255)
                                                                                                            gcgTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGCTTATGCCGCATCCGACATc                                                                                                                                                                         <  1:341927/289‑1 (MQ=255)
                                                                                                            cgcaatagcctgatttagcgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaacgcctgatgcgacgcttaacgcgtcttatcaggcctacgccagacagcgcaatagcctgatttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                                                                                                                                                           <  1:2169717‑M1/31‑1 (MQ=255)
                                                                                                            cgcaatagcctgatttagcgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaacgcctgatgcgacgcttaacgcgtcttatcaggcctacgccagacagcgcaatagcctgatttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                                                                                                                                                           >  2:2169717‑M1/178‑208 (MQ=255)
                                                                                                                     gcTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCACGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGCAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGAAAGCCCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCACCTTCCGACATCCACGCCTGa                                                                                                                                                                >  1:1977263/1‑289 (MQ=255)
                                                                                                                          aCGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGa                                                                                                                                                           <  2:1351691/289‑1 (MQ=255)
                                                                                                                              cgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaacgcctgatgcgacgcttaacgcgtcttatcaggcctacgccagacagcgcactagcctgatttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcCATGCCTGATGCGACGCTTGCCGCGTCTTACCAGGCCTACGCTAGACATCGCAATAGTCTGACATAGCGTGATTTTGTACGTCGGATAATGCGATTACGCCTCATCCACCATCAACgactgctgccac                                                                                                                                                          <  1:802830‑M1/128‑4 (MQ=255)
                                                                                                                                  ggcagtgtgcTGGGTGTTCTTGTGATCGCCAAGTGGGAACACAAATTTAACCTTAAGAAACGGCTGGCTTATGGGTGTGAAGTGCGTGGCGAATTGGATAAAACGGCGGATCAAGAATTGAAAATTGCCGTGGATATCCACCCTGCAATGAGTAAATGCCTGATGCGACGCTTGCCGCGGCGTATCAGGCCTACGCCATACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCTTCCGACATCATCGCCTGATGCGACGCTTaa                                                                                                                                                    <  1:1340967/280‑1 (MQ=18)
                                                                                                                                   gTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAc                                                                                                                                                   >  2:736259/1‑288 (MQ=255)
                                                                                                                                              gCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAg                                                                                                                                       >  2:1849800/1‑289 (MQ=255)
                                                                                                                                                 gTGCGGGGGATTGCCAAGTGGGAAAACAAATTTGACCGTAAGAAGGCGGTGGCTGATGGAGTGGAAGGGCGGGGGAAAGTTGATAAAACTGCGGATTAATAATTGAAGATTGCCGGGGATAGCCACCCGGCAATGTGGGAATGCCGGAGGCGACGCGTGCCGGGTCTTATCAGGCCTACGCCAGACGGCGAAATAGCCTGATTTAGCGTGATGTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCATGATGCGACGCTTAACGCGTCTTATCAGGc                                                                                                                                     <  1:88932/288‑1 (MQ=18)
                                                                                                                                                   gCTGGGGATTGACAAGTGGGAATAAAAATTTGACCGTAAGAACAGGCGTGCTTATGTGCGTGAAGTGCCGGGCAAAGTTGATAAAACGGTGGATGAATAATTGAAGATTCCCGTGGTTATGCACCCTGCAATGTGTGAATGCCAAATGGGACGCTTGCCGCGGCTGATCAGGCCGACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGACTGATgtcggatgcggcataaacgccttatccg                                                                                                                                   <  1:1354172/288‑28 (MQ=18)
                                                                                                                                                     tGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACg                                                                                                                                <  2:2426919/289‑1 (MQ=255)
                                                                                                                                                      ggTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACg                                                                                                                                <  2:898234/288‑1 (MQ=255)
                                                                                                                                                          tatgccgcatccgacatcaacgcctgatgcgacgcttaacgcgtcttatcaggcctacgccagacagcgcaatagcctgatttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcAATGCCTGATGCGACg                                                                                                                                                                                                                                                                          >  1:2215843‑M1/132‑147 (MQ=255)
                                                                                                                                                          tatgccgcatccgacatcaacgcctgatgcgacgcttaacgcgtcttatcaggcctacgccagacagcgcaatagcctgatttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcAATGCCTGATGCGACGCTTGc                                                                                                                                                                                                                                                                     <  2:1383023‑M1/21‑1 (MQ=255)
                                                                                                                                                           ttGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGTGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGAAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGa                                                                                                                           <  2:181146/288‑1 (MQ=255)
                                                                                                                                                            tGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCTACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGt                                                                                                                                                                                                        >  1:729095/1‑210 (MQ=255)
                                                                                                                                                            tGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGa                                                                                                                                                                                                                  <  1:2455229/200‑1 (MQ=255)
                                                                                                                                                                  ttGGGACCACAAATTTGACCGTAAGAAAGCGCTTGCTTATGGGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGTGGATATCCACCCGGCAATGTATGAATGCCTGATGCGACGCTTGCCGCGTCTTAGCAGGGCTACGCCAGACAGCGCAATAGCCTGATTTAACGTGATTTTGTAGGTCGAATAAGGCGTTTATGCCGCATCCGACATCAACGCCGGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCaa                                                                                                                   <  2:2100130/288‑1 (MQ=21)
                                                                                                                                                                    gggACCACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCTTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATGGAAGATTGCCGGGGCTATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCaa                                                                                                                   <  2:2584691/287‑1 (MQ=255)
                                                                                                                                                                       aaCACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGttt                                                                                                                                                                                          <  2:1881420/213‑1 (MQ=255)
                                                                                                                                                                        acccaAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCATGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCAAGACAGCGCAATAg                                                                                                                >  2:2371279/4‑286 (MQ=255)
                                                                                                                                                                             aaTTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGa                                                                                                                           <  1:1394768/270‑1 (MQ=255)
                                                                                                                                                                             aaTTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAttt                                                                                                        >  2:1679666/1‑289 (MQ=255)
                                                                                                                                                                              cgcctgatgcgacgcttaacgcgtcttatcaggcctacgccagacagcgcaatagcctgatttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                                                                                                                                                      >  1:188745‑M1/112‑147 (MQ=255)
                                                                                                                                                                              cgcctgatgcgacgcttaacgcgtcttatcaggcctacgccagacagcgcaatagcctgatttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                                                                                                                                                      <  2:188745‑M1/36‑1 (MQ=255)
                                                                                                                                                                                         aGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCg                                                                                                                                                                                                                     >  1:261534/1‑168 (MQ=255)
                                                                                                                                                                                               cgcTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATaa                                                                                                                                                                                                 >  1:326248/1‑182 (MQ=255)
                                                                                                                                                                                                 cTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGc                                                                                                                                                             <  2:1625708/216‑1 (MQ=255)
                                                                                                                                                                                                 cTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGt                                                                                                                                               >  1:1716182/1‑230 (MQ=255)
                                                                                                                                                                                                  tGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCaa                                                                                                                                                                                                                                    >  1:857327/1‑144 (MQ=255)
                                                                                                                                                                                                  tGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGt                                                                                       >  2:2462753/1‑285 (MQ=255)
                                                                                                                                                                                                              gTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCg                                                                                                                                                                                    >  1:2534983/1‑180 (MQ=255)
                                                                                                                                                                                                                   cgccagacagcgcaatagcctgatttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACATCGCAATAGCCTGATTTAGCGTGATTTTGTATGTCGGATCCGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTCTCATGCCTACGCCACTCAGCGCACAAGCCTGATTTATCATGATTTTGTAGTTCCGATAAGGCTTTTAcgcc                                                                    <  1:381939‑M1/214‑1 (MQ=255)
                                                                                                                                                                                                                       cTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCAt                                                                >  2:1189078/1‑288 (MQ=255)
                                                                                                                                                                                                                        gacagcgcaatagcctgatttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                                                                                                                                                                                                                                       <  2:2329155‑M1/35‑1 (MQ=255)
                                                                                                                                                                                                                         gggAAAAGTTGAGAAACCTGCGGGTTAATAATTGAAGATGCCCGGGGATATCCACCCGGGAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCGACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCg                                                             <  1:274485/289‑1 (MQ=17)
                                                                                                                                                                                                                         ggagaaaTTTGATAAAAGTGCGGATCAATTATTGAGGGGTGCCGGGGAGCTCCACCCGGACATGGTTGAATGCCTGATGCTGCGCTTGCCGCGGCTTATCAGGCCTACGCCAGGCAGCGAAATAGCCTGATTTAGCGTGGTTTTGTGGGTCCGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCg                                                             <  1:2360705/285‑1 (MQ=14)
                                                                                                                                                                                                                          ggCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                                                                                                                                                      <  2:317779/103‑1 (MQ=255)
                                                                                                                                                                                                                          ggCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCa                                                                                                                                                                                                                                              <  2:2402698/111‑1 (MQ=255)
                                                                                                                                                                                                                             cgcaatagcctgatttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATACGGCGTTTATGCCGCATCCGACCTCAACGACTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTTCTTTCGCTTTATTTTGTATGTCGGATAAGGCGTTTCCGCCGCATCCGACa                                                          <  1:461971‑M1/224‑1 (MQ=255)
                                                                                                                                                                                                                             cgcaatagcctgatttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACCCGTCTTATCAGGCCTACGTCAGACAGCGCAATCGCCTGGTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACa                                                          <  2:2642825‑M1/224‑1 (MQ=255)
                                                                                                                                                                                                                                    aTAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                                                                                                                                                      <  2:861109/93‑1 (MQ=255)
                                                                                                                                                                                                                                       tgatttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGa                                               <  1:2317365‑M1/235‑1 (MQ=255)
                                                                                                                                                                                                                                           tGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTaa                                                                                                                                                    <  2:2291923/184‑1 (MQ=255)
                                                                                                                                                                                                                                             agcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTTCATTAGCTTTATTTTGTACGTCGGATCAGGCGTTTCCGCCGCATCCGACATCCATGCCTGATGCGAc                                         <  1:2135778‑M1/241‑1 (MQ=255)
                                                                                                                                                                                                                                                   ccgttgatgtgttttgagtagcgacccACCCGTCTAAGTGTGAATGCCTGAGGCGACGCTTGCCGCTTCTTATCAGGCCAACGCCAGAAAGCTCAATCGCCTGTTTTTTCGTGAGTTTGAGGATCGTATAAGGCTTTTATGCCTCAGCCGACATCAACGCCTGATGCGACGCTTATCTCGTCTTATCAGTCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGTATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGc                                   <  1:551137/264‑1 (MQ=11)
                                                                                                                                                                                                                                                   aataatgtgaGATTGCCCGGTAGATCCACCCGGCAATGGGTGAATGCCTGATGCGACGCGTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGAAATAGCCGGATTTAGCGTGATTTTGTAGGTCGGATAGGGCGGTTAGGCCGCAGCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGc                                   <  1:1865466/280‑1 (MQ=14)
                                                                                                                                                                                                                                                   aataatTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGc                                   <  1:2128333/289‑1 (MQ=255)
                                                                                                                                                                                                                                                        ttGAAAGTTGCCGGGGATATCCACCCGGCAAGGTGTGAATGCCTGATGCGACGCTTGCCGCGTGTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGAAATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGt                              <  2:1960622/289‑1 (MQ=255)
                                                                                                                                                                                                                                                          gAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATaa                                                                                                                                                                                                 <  2:283389/124‑1 (MQ=255)
                                                                                                                                                                                                                                                          gAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCt                            >  2:2246798/1‑289 (MQ=255)
                                                                                                                                                                                                                                                          gAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGAAGCTTGCCGCGTCt                            >  2:47987/1‑289 (MQ=255)
                                                                                                                                                                                                                                                            aGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc                                                                                                               >  2:1293982/1‑204 (MQ=255)
                                                                                                                                                                                                                                                            aGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc                                                                                                               <  1:1293982/204‑1 (MQ=255)
                                                                                                                                                                                                                                                             gATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCa                                                                                                                             >  2:2134081/1‑189 (MQ=255)
                                                                                                                                                                                                                                                             gATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTa                          >  2:1760191/1‑288 (MQ=255)
                                                                                                                                                                                                                                                             cagtgtaggtgttttagtccgcttccgatatgAAGGCCTGATGCTCAGCTTGCCGCGTCTTTTCAGGCCTACGCCAGACAGCTCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCCTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt                         >  1:2246798‑M1/33‑289 (MQ=255)
                                                                                                                                                                                                                                                              atggCCAGGGATATCCCGCGGGCAAGTTGTGAGTACCGGCTGCACCTCTTGCGACGTCTTGTCAGGCCTACGCCAGCAAGCGCAATAGCCGGATTTGGCGTGATTTTGTAAGGCGGATAAGGCGTTTATGCCGCATCATCCATCAGCGCCGGATGCGACGCTTAACCCGTCTTATCAGGCCTACCCCAGAGAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCTACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATc                        <  1:1509182/286‑1 (MQ=11)
                                                                                                                                                                                                                                                                tGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAgg                                                                                                                                                                                               <  1:609078/120‑1 (MQ=255)
                                                                                                                                                                                                                                                                 gCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTaa                                                                                                                                                    >  2:2602084/1‑162 (MQ=255)
                                                                                                                                                                                                                                                                   cGGGGATATCCACCCGGCAATGTGTTAATGCCTGAGGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                    <  2:1977263/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                   cGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                    <  2:1279262/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                   cGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                    <  2:1709802/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                   cGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                    <  2:2063656/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                           tacgccgcatccgacatcAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                                                                                                                                                      >  2:2706072‑M1/19‑54 (MQ=255)
                                                                                                                                                                                                                                                                                ccGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGAAATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgc      >  2:770799/1‑289 (MQ=39)
                                                                                                                                                                                                                                                                                       tgtgtgAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTAt                         >  2:551137/1‑263 (MQ=37)
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CGTGCCTGGCGTGTCGTTTGTGGTGTTGCTGGCAACGCTGGGTAGCGTAGGTATTCCGCTGGAAGGTCTGGCGTTTATTGCTGGTGTTGACCGTATCCTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAA  >  NC_000913/4295547‑4296110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: