BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	65,670	T→G	11.0%	A37A (GCA→GCC)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,670	0	T	G	11.0%	264.5 / 2.1	103	A37A (GCA→GCC)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand): ref base T (19/69); new base G (11/4); total (30/73)
Fisher's exact test for biased strand distribution p-value = 1.60e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

CATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCA  >  NC_000913/65433‑65907

cATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGTCACCTTCACGCAGGAGCGTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTg                                                                                                                                                                                                                                                    <  2:847692/239‑1 (MQ=255)

cATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGGATAAACGCCACGGACTCTTGCGGTg                                                                                                                                                                                                                                                    >  2:573835/1‑239 (MQ=255)

cATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTg                                                                                                                                                                                                                                                    <  2:793882/239‑1 (MQ=255)

cATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTGTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCGTGCGGTg                                                                                                                                                                                                                                                    <  1:724022/239‑1 (MQ=255)

cATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACAGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTg                                                                                                                                                                                                                                                    <  2:1171004/239‑1 (MQ=255)

       tAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑g                                                                                                                                                                                                                                             <  1:352082/238‑1 (MQ=255)

        aGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAAAGCCACGGAGCATTGCGGGGCCAGC‑GTa                                                                                                                                                                                                                                           >  2:698281/1‑239 (MQ=255)

          cGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATTAACGCCCCGGGCTCTTGCGGGGCAAGC‑GTaa                                                                                                                                                                                                                                          >  2:345649/1‑238 (MQ=255)

            ttCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCTGGGATAAAACCCCCCGCCTCTTTCAGTGCCAGC‑GGaaacc                                                                                                                                                                                                                                       >  2:527518/1‑236 (MQ=255)

            ttCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTaa                                                                                                                                                                                                                                          >  2:432847/1‑236 (MQ=255)

                ggCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGAGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATTCTGAGCGCGGGGAA‑CCTGATCGGCGGGGAAAAACGCCACGGAATCTTGCGGGGCAAGC‑GCTACCTGcc                                                                                                                                                                                                                                    >  2:90454/1‑237 (MQ=255)

                   ggACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACg                                                                                                                                                                                                                                 <  2:577615/238‑1 (MQ=255)

                     aCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGc                                                                                                                                                                                                                               <  2:1139226/238‑1 (MQ=255)

                           aTCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGCAAATCCTTTAACGCCAGCGGGGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGGATAAACGCCCCGGGGCCTTGTGGTGCCGGC‑GCAACCTGCCACGGC‑GCGTTGt                                                                                                                                                                                                                       >  1:1139226/1‑239 (MQ=255)

                           aTCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAAAGCCACGGACTCTTGCGGGGCAAGC‑GTAAACTGCAACGGC‑CCGTTg                                                                                                                                                                                                                        >  2:666477/1‑238 (MQ=255)

                                  tCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCgc                                                                                                                                                                                                                <  2:645352/239‑1 (MQ=255)

                                  tCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCgc                                                                                                                                                                                                                <  1:1104748/239‑1 (MQ=255)

                                     tAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGGATAAACGCCCCGGACTCTTGCGGTGCCAGC‑GTAACCTGCAACAGC‑CCGTTGTCCGTCgccc                                                                                                                                                                                                              >  2:1186611/1‑237 (MQ=255)

                                     tAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAACGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGGATAAACGCCCACGACTCCTGCGGTGGCAGC‑GGAACCTGCAAAGGCGCCGTTGccctcccac                                                                                                                                                                                                               >  1:834440/1‑232 (MQ=255)

                                      aGACGGTAACGCCACCTTCACTCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCgccag‑c                                                                                                                                                                                                           <  1:421300/239‑1 (MQ=255)

                                         cGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCAGCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGccag‑ccag                                                                                                                                                                                                        <  2:736240/239‑1 (MQ=255)

                                              cctcccccTTCCCGCTGGCGTTTTTCTCAATTCATCAATTGGCGATGGGCGCGACAGTAAACGCCATACACCGGCTGGCGGTGAAAAGCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATAGGCTGAGCGCGGGGAA‑CCTTATCGGCGGCAATAAACGCCACGGACTCTGGCGGTGCAATC‑GTATCCTGCAACGGC‑CCGTTGTTCGTCGCCAG‑GCAGAAGga                                                                                                                                                                                                   <  1:122826/233‑1 (MQ=255)

                                               cGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGGATAAACCCCACGGACTCTTGCGGGGCAACG‑GTAACCTGCCACGGC‑CCGGTGTCCGTCGCCCG‑CCCGAAGgag                                                                                                                                                                                                  >  2:445465/1‑239 (MQ=255)

                                                     ctgcACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCt                                                                                                                                                                                            <  1:740385/236‑1 (MQ=255)

                                                       tCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGt                                                                                                                                                                                          <  2:834440/239‑1 (MQ=255)

                                                          cGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGGATAAAAGCCCCGGACTCTTTCGGGGCAAGC‑GGAACCCGCAACGGC‑CCGTTTGCCGGCGCCCG‑CCCGaaagggagttttttcc                                                                                                                                                                                        >  1:239215/1‑224 (MQ=255)

                                                             aGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACTGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCtt                                                                                                                                                                                     <  1:70348/238‑1 (MQ=255)

                                                                     ttCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTg                                                                                                                                                                                                                                                    <  1:632923/170‑1 (MQ=255)

                                                                          aaTTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGc                                                                                                                                                                      <  1:611425/239‑1 (MQ=255)

                                                                          aaTTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGc                                                                                                                                                                      <  1:1092034/239‑1 (MQ=255)

                                                                               atcaATTGGCGATGGGTGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGGATAAAAGCCCCGGGCCCTTTCGGGGGCAAC‑GGAAACTCCAACGGG‑CCCGTGTCCGCCGCCCGCCCAGAGGGGGAC‑TCTTTCCCCTGCCGGGGGGTCCCGcactg                                                                                                                                                                  >  1:309743/1‑235 (MQ=255)

                                                                               atcaATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGGCTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCCTG‑CGGGGTGTTCCCCCCgtg                                                                                                                                                                 >  2:774711/1‑239 (MQ=255)

                                                                                tcaATTGGCGATGGGCGCGACAGTAAAGGCCATACCCCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAgtgt                                                                                                                                                                <  2:727452/239‑1 (MQ=255)

                                                                                tcaATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAgtgt                                                                                                                                                                <  1:1074077/239‑1 (MQ=255)

                                                                                 caATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGGATAAAAGCCCCCGACTCTTTCGGGGGCAGC‑GTAACCTTCCAAGGC‑CCCGTTTCCGTCGCCAG‑CCCGAAGGAGACTTCTGTCCCTTG‑CGGGGGGTCCCCCCacgggc                                                                                                                                                               >  1:750876/1‑234 (MQ=255)

                                                                                 caATTGGCGATGGGCGCAACAGGAAGGGCCAGACCCGGGCTGGCGGTGAAAATCCTTTAACGCCAGGGGTGTCAGGCGAAAGCCTGTTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGAGCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CAGTTGTCAGGCGCGAG‑CAAGAAGGAGGCTTCTGTCACTTG‑CGGGGTGTCCCGCCAgtgt                                                                                                                                                                <  1:320451/238‑1 (MQ=255)

                                                                                       gCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGtt                                                                                                                                                          <  1:807880/238‑1 (MQ=255)

                                                                                       acGATGGGCGCGAAAGTAAAGGGCATACACCGGTGAGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATAGGCTGAGGGCGGGGAA‑CCTGGACGGGGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGtt                                                                                                                                                          <  1:250284/237‑1 (MQ=255)

                                                                                             ggCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGt                                                                                                                                                                                          <  2:734756/201‑1 (MQ=255)

                                                                                                gcgACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATaaaa                                                                                                                                                 <  2:750876/238‑1 (MQ=255)

                                                                                                 cgACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGGAAAAACGCCACGGACTCTTTCCGGGCAAGCGGTACCCGGCAA‑GGC‑CCCGTGTCCGGCCCCCG‑CCCGAAAGAGACCTCTGTCCCTTT‑GGCGGGGTCCCGcccggggtgggtgtagagaaaaa                                                                                                                                                >  1:938022/1‑217 (MQ=255)

                                                                                                       tAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGGATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTTTCCCTTG‑CGGGGGGTCCCGCCAGGGTCGGGGTAAGATTAAAcacgc                                                                                                                                            >  2:131340/1‑232 (MQ=255)

                                                                                                             ccATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTc                                                                                                                                                                                                                      <  2:1131522/158‑1 (MQ=255)

                                                                                                                tACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCCCGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCCGAAGGAGACTTCTGTCCCTTG‑CGGGGGGTCCCGCCAGTGTCGGGGTAAGATAAAACCTGCCTGCGcccc                                                                                                                                   >  2:290974/1‑234 (MQ=255)

                                                                                                                         tGGCGGTGAAAATCCTTTAACGCCAGCGGGGTCAGGCGAAAGCCTTGTTCCCCCTGCAAAATATGCTGAGCGGGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATcc                                                                                                                        <  2:954649/238‑1 (MQ=255)

                                                                                                                              gTGAAAATCCTTTAACGCCAGCGGTGGCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCGGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCaa                                                                                                                   <  1:554925/238‑1 (MQ=255)

                                                                                                                                  aaaTCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAAc                                                                                                             <  2:554161/239‑1 (MQ=255)

                                                                                                                                    aTCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCa                                                                                                           <  2:521864/239‑1 (MQ=255)

                                                                                                                                    aTCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCa                                                                                                           <  1:492140/239‑1 (MQ=255)

                                                                                                                                    aTCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCa                                                                                                           <  1:1163211/239‑1 (MQ=255)

                                                                                                                                      ccTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAgg                                                                                                         <  2:371735/239‑1 (MQ=255)

                                                                                                                                        tttACCGCCAGCGGTGTCAGGCAAAAGCCTTTTTCCCCCTCCAAAATATGCTGCGGGCTGGAAA‑CCTGACCGTCGGAAATAAACGCCGCGGACTCTTGGGGTGCAAGC‑GTACCCTGCAACGGC‑CCGTTGCCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCt                                                                                                       <  1:573835/239‑1 (MQ=255)

                                                                                                                                        tttAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGTCTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCt                                                                                                       <  2:309743/239‑1 (MQ=255)

                                                                                                                                        tttAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCt                                                                                                       <  2:1032062/239‑1 (MQ=255)

                                                                                                                                           aaCGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTCCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCCCGGGCTCTTGCGGGGAAAGG‑GGAACCCGCAACGGC‑CCGTTGTCCGCCGCCCG‑CCGGAACGGGACTTATGTCCGCTG‑CGGGGGGTCCCGCCCGGTCCGGGGTTAGATAAAATCTGCCTGCGCCCCGCGGTGAATCCCCACAA‑AAACCCGGCCTGa                                                                                                    >  1:183831/1‑239 (MQ=255)

                                                                                                                                              gCCAGTGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGta                                                                                                 <  2:299243/239‑1 (MQ=255)

                                                                                                                                                 aGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCCCGGGCTCTTGCGGTGCAAGC‑GGCACCTCCAACGGC‑CCCTTGTCCGCCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGGGGCCCGCCAGTGCCGGGGTCAGATAAAAAACGCCCGCCGCCCCCCGAAAAACCATCAAACAAACCAGCCTGGAgagt                                                                                                >  1:63892/1‑235 (MQ=255)

                                                                                                                                                                     ttGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTTCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGGTGTCCGGCGCCCG‑CCAGAAGGAGGCTTCTGTCCCCTG‑CGGGGGGTCCCGCCAGTGTCGGGGTAAGATAAAAACCGCCTGCGCCACGCCGGAAATCCATCCAA‑AAACCCGGCTTGATGAT‑AGCCTGGGTTCGTTTGAttt                                                                           >  1:717109/1‑236 (MQ=255)

                                                                                                                                                                       gtgcACCCTGCAAAATATGCTGGGCGCGGGGAA‑CCTGAGCGGCGGAAAAAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCtg                                                                       <  1:666477/236‑1 (MQ=255)

                                                                                                                                                                       gTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCtg                                                                       <  2:223279/239‑1 (MQ=255)

                                                                                                                                                                       gTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCtg                                                                       <  2:912418/239‑1 (MQ=255)

                                                                                                                                                                       gTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCtg                                                                       <  1:432847/239‑1 (MQ=255)

                                                                                                                                                                       gTTCACCCTGAAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTCCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCtg                                                                       <  2:466032/239‑1 (MQ=255)

                                                                                                                                                                       gTTCACCCCGCAAAATATGCTGGGCGGGGGGAA‑ACTGATGGGGGGGAAAAAACGCCACGGCCTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCtg                                                                       <  2:77825/239‑1 (MQ=255)

                                                                                                                                                                       gTTAACCCTGCAAAATGAGGGGGGCGCGGGAAA‑CCTGATCGGCGGGAAGAAACGCCAGGGACTCTGGCGGTGCAAGC‑GTAGCCGGCAACGGC‑CGGTTGCCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑GGGGGTGTCCCGCCAGGGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCtg                                                                       <  1:970179/239‑1 (MQ=255)

                                                                                                                                                                        ttCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGGGCAGGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CAAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCtg                                                                       <  1:1085201/238‑1 (MQ=255)

                                                                                                                                                                         tCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCtgtg                                                                     <  2:717109/239‑1 (MQ=255)

                                                                                                                                                                             ccTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGGGCAAGC‑GGAAACTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGGTAAGATAAAAACTGCCTGCGCCCCGCTGAAAATCCATCAAA‑AAACCACGGTTGAGGAT‑AGCCCGGTTTCGTTTGATTGGGTGTGGttt                                                                 >  2:613711/1‑239 (MQ=255)

                                                                                                                                                                              cTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGGTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGGGTCCCGCCCGTGTCGGGTTAAAATAAAACCTGCCCGCGCCCCCGTGAAAATCCATCAAA‑AAACCAGGCTTGGGGAT‑AGCCCGGGTTCGGTTGGTTGGGTGTGGtttt                                                                >  1:486040/1‑239 (MQ=255)

                                                                                                                                                                                gCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTGGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTtat                                                              <  1:83198/239‑1 (MQ=255)

                                                                                                                                                                                gCAAAATATGCTGAGCGCGGGGAA‑CCGGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTtat                                                              <  2:571438/239‑1 (MQ=255)

                                                                                                                                                                                  gactgaggCTGGGCGCGGGGAA‑CCTGAGCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTtat                                                              <  2:358333/230‑1 (MQ=255)

                                                                                                                                                                                  aaaaaaTGCTGAGCGGGGGGAA‑CCGGATCGGCGGGAATAAGCGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGCCTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATAc                                                            <  1:571033/239‑1 (MQ=255)

                                                                                                                                                                                  aaaaTATGCTGTGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CGGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATAc                                                            <  2:1162343/239‑1 (MQ=255)

                                                                                                                                                                                       atGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTg                                                                                                                                                                                                                                                    <  1:1049470/56‑1 (MQ=255)

                                                                                                                                                                                       atGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTg                                                                                                                                                                                                                                                    >  2:1049470/1‑56 (MQ=255)

                                                                                                                                                                                           tGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAGCGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTAc                                                   <  2:743436/239‑1 (MQ=255)

                                                                                                                                                                                           tGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTa                                                    <  2:479397/238‑1 (MQ=255)

                                                                                                                                                                                           tGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAAAGGC‑CCGTTGTCCGCCGCCAG‑CCAGAAGGAGACTTCTGTCCCCTG‑CGGGGGGTCCCGCCCGTGTCGGGGTACAATAAAAACTGCCTGAGGCACGCTGCAAATCCATCCAA‑CCACCACGCCTGGGTATAAGCCGGGGTATG‑TTGATGTGATGTTGTTTTATACAgatatt                                                     >  1:375826/1‑232 (MQ=255)

                                                                                                                                                                                            gAGCGGGGGGAA‑CCTGAACGGAGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTGGCCAG‑CCAGAAGTAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACt                                                  <  2:687812/239‑1 (MQ=255)

                                                                                                                                                                                                gcgGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGcc                                               <  2:486040/238‑1 (MQ=255)

                                                                                                                                                                                                gcgGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTGGCGGGGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGcc                                               <  2:183831/238‑1 (MQ=255)

                                                                                                                                                                                                gcgGGGAA‑CCGGGCGGGCGGGAATAAACGCCACGGACTCTGGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTGG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGcc                                               <  1:345649/238‑1 (MQ=255)

                                                                                                                                                                                                cgggggAA‑ACTGAGGGGAGAGAATAAAGGCGACGTACGCATGCGGGCCAAGG‑GCAACGTGCAGCGGC‑CGGTGGTCGGTCGCCAT‑CCAGAAGGAGACTTCGGTCCCGTG‑CGGTGTATCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGcc                                               <  1:1154651/236‑1 (MQ=255)

                                                                                                                                                                                                 gggggAACCCTGACCGGGGGGAATAACCGCCCCGGCCTCTTGCGGTGCAAGC‑TTACCCGGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGcc                                               <  1:290974/237‑1 (MQ=255)

                                                                                                                                                                                                      aa‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCtg                                                                       <  2:358197/208‑1 (MQ=255)

                                                                                                                                                                                                               gggcgtgaaaacacccaccGGACTGTGGCGGTGAAAGC‑GGAACCTGCACCGGG‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTGCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTcg                                <  1:1123775/221‑1 (MQ=255)

                                                                                                                                                                                                                   gggAATAAGCGCCACGGCCTCTTGCGGTGCAAGC‑GTACCCGGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCgcgc                             <  2:1065804/238‑1 (MQ=255)

                                                                                                                                                                                                                   gggAATAAACGCCACGGCCTCTGGCGGTGCAGGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCGGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCgcgc                             <  1:131340/238‑1 (MQ=255)

                                                                                                                                                                                                                   gggAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCgcgc                             <  2:182735/238‑1 (MQ=255)

                                                                                                                                                                                                                          aaCGCCACGGACTCTTGCGGGGCACGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTa                     <  1:613711/239‑1 (MQ=255)

                                                                                                                                                                                                                                acggtcttgtGCGGTGCAAGC‑GTAACCTGCACCGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGCCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGa               <  2:525999/230‑1 (MQ=255)

                                                                                                                                                                                                                                aCGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGa               >  2:1105843/1‑239 (MQ=255)

                                                                                                                                                                                                                                aCGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGa               <  2:778961/239‑1 (MQ=255)

                                                                                                                                                                                                                                aCGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGGTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGa               >  1:612840/1‑239 (MQ=255)

                                                                                                                                                                                                                                 cggtccctcgCGGTGCAAGC‑GAAACCGGCAACGGC‑CCGTTGGCCGCCGCCGG‑CCAGAAGGAGACTTCTGTCCCTGG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGAt              <  1:445465/230‑1 (MQ=255)

                                                                                                                                                                                                                                 cGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGAt              <  2:938022/239‑1 (MQ=255)

                                                                                                                                                                                                                                         tGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTa      >  2:231298/1‑239 (MQ=255)

                                                                                                                                                                                                                                              tGCAAGC‑GTACCCTGCAGCGGC‑CCGTTGTCCGCCGCCAG‑CCAGAAGGAGACTTCTGGCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTa                                                    <  1:1143557/188‑1 (MQ=255)

                                                                                                                                                                                                                                              tGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGGAAATCCATCCAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGCTTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGATTACGCAGATAGTGTTTATCca  >  2:143049/1‑238 (MQ=255)

CATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAA‑CCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGC‑GTAACCTGCAACGGC‑CCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGTCCCTTG‑CGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAA‑AAACCAGGCTTGAGTAT‑AGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCA  >  NC_000913/65433‑65907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`